CN114072520A - 检测靶核酸片段的方法及试剂盒 - Google Patents

检测靶核酸片段的方法及试剂盒 Download PDF

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Publication number
CN114072520A
CN114072520A CN202080047412.4A CN202080047412A CN114072520A CN 114072520 A CN114072520 A CN 114072520A CN 202080047412 A CN202080047412 A CN 202080047412A CN 114072520 A CN114072520 A CN 114072520A
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nucleic acid
acid fragment
target nucleic
protein
crispr
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Chinese (zh)
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渡边力也
濡木理
西增弘志
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RIKEN
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RIKEN
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
CN202080047412.4A 2019-07-04 2020-07-06 检测靶核酸片段的方法及试剂盒 Pending CN114072520A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2019-125564 2019-07-04
JP2019125564 2019-07-04
PCT/JP2020/026371 WO2021002476A1 (ja) 2019-07-04 2020-07-06 標的核酸断片の検出方法及びキット

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CN114072520A true CN114072520A (zh) 2022-02-18

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US (1) US12522859B2 (https=)
EP (1) EP3995833A4 (https=)
JP (1) JP7579577B2 (https=)
CN (1) CN114072520A (https=)
CA (1) CA3143923A1 (https=)
WO (1) WO2021002476A1 (https=)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114072520A (zh) 2019-07-04 2022-02-18 国立研究开发法人理化学研究所 检测靶核酸片段的方法及试剂盒
US20240094199A1 (en) * 2020-12-28 2024-03-21 Riken Method and kit for detecting target nucleic acid fragment
CN113373264B (zh) * 2021-06-10 2022-04-12 安徽医科大学 一种基于CRISPR/Cas系统的新型冠状病毒双靶标快速检测方法及试剂盒
US20230279375A1 (en) * 2021-12-13 2023-09-07 Labsimply, Inc. Signal boost cascade assay
JPWO2023195542A1 (https=) * 2022-04-08 2023-10-12
WO2024072775A1 (en) * 2022-09-26 2024-04-04 The Johns Hopkins University Devices and systems for dna capture
JPWO2024111543A1 (https=) * 2022-11-25 2024-05-30

Citations (2)

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Publication number Priority date Publication date Assignee Title
CN109652295A (zh) * 2014-01-31 2019-04-19 凸版印刷株式会社 生物分子分析试剂盒及生物分子分析方法
WO2019126577A2 (en) * 2017-12-22 2019-06-27 The Broad Institute, Inc. Crispr effector system based multiplex diagnostics

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JP3727026B2 (ja) 2003-04-10 2005-12-14 博行 野地 一分子酵素活性検出に用いられるマイクロチャンバと１０００ｆＬ以下の液滴を調製する方法
WO2008105814A2 (en) * 2006-08-22 2008-09-04 Los Alamos National Security, Llc Miniturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids
US10337051B2 (en) * 2016-06-16 2019-07-02 The Regents Of The University Of California Methods and compositions for detecting a target RNA
CN111479928A (zh) 2017-11-17 2020-07-31 凸版印刷株式会社 靶分子的检测方法
US10253365B1 (en) * 2017-11-22 2019-04-09 The Regents Of The University Of California Type V CRISPR/Cas effector proteins for cleaving ssDNAs and detecting target DNAs
JP2019125564A (ja) 2018-01-19 2019-07-25 株式会社豊田自動織機 蓄電モジュール
CA3119971A1 (en) 2018-11-14 2020-05-22 Pardis SABETI Multiplexing highly evolving viral variants with sherlock
CN114072520A (zh) 2019-07-04 2022-02-18 国立研究开发法人理化学研究所 检测靶核酸片段的方法及试剂盒
US20240094199A1 (en) * 2020-12-28 2024-03-21 Riken Method and kit for detecting target nucleic acid fragment

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109652295A (zh) * 2014-01-31 2019-04-19 凸版印刷株式会社 生物分子分析试剂盒及生物分子分析方法
WO2019126577A2 (en) * 2017-12-22 2019-06-27 The Broad Institute, Inc. Crispr effector system based multiplex diagnostics

Non-Patent Citations (2)

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Title
ASIELLO P.J.等: "Miniaturized isothermal nucleic acid amplification, a review", 《LAB ON A CHIP》, vol. 11, no. 8, pages 1420 - 1430 *
ZANOLI L.等: "Isothermal amplification methods for the detection of nucleic acids in microfluidic devices", 《BIOSENSORS》, vol. 3, no. 1, pages 18 - 43, XP055412468, DOI: 10.3390/bios3010018 *

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US20220348993A1 (en) 2022-11-03
CA3143923A1 (en) 2021-01-07
US12522859B2 (en) 2026-01-13
JP7579577B2 (ja) 2024-11-08
EP3995833A1 (en) 2022-05-11
JPWO2021002476A1 (https=) 2021-01-07
WO2021002476A1 (ja) 2021-01-07
EP3995833A4 (en) 2023-08-09

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