CA3143923A1 - Method and kit for detecting target nucleic acid fragment - Google Patents
Method and kit for detecting target nucleic acid fragment Download PDFInfo
- Publication number
- CA3143923A1 CA3143923A1 CA3143923A CA3143923A CA3143923A1 CA 3143923 A1 CA3143923 A1 CA 3143923A1 CA 3143923 A CA3143923 A CA 3143923A CA 3143923 A CA3143923 A CA 3143923A CA 3143923 A1 CA3143923 A1 CA 3143923A1
- Authority
- CA
- Canada
- Prior art keywords
- nucleic acid
- acid fragment
- target nucleic
- protein
- crispr
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2019-125564 | 2019-07-04 | ||
| JP2019125564 | 2019-07-04 | ||
| PCT/JP2020/026371 WO2021002476A1 (ja) | 2019-07-04 | 2020-07-06 | 標的核酸断片の検出方法及びキット |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3143923A1 true CA3143923A1 (en) | 2021-01-07 |
Family
ID=74101362
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3143923A Pending CA3143923A1 (en) | 2019-07-04 | 2020-07-06 | Method and kit for detecting target nucleic acid fragment |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US12522859B2 (https=) |
| EP (1) | EP3995833A4 (https=) |
| JP (1) | JP7579577B2 (https=) |
| CN (1) | CN114072520A (https=) |
| CA (1) | CA3143923A1 (https=) |
| WO (1) | WO2021002476A1 (https=) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20230279375A1 (en) * | 2021-12-13 | 2023-09-07 | Labsimply, Inc. | Signal boost cascade assay |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114072520A (zh) | 2019-07-04 | 2022-02-18 | 国立研究开发法人理化学研究所 | 检测靶核酸片段的方法及试剂盒 |
| US20240094199A1 (en) * | 2020-12-28 | 2024-03-21 | Riken | Method and kit for detecting target nucleic acid fragment |
| CN113373264B (zh) * | 2021-06-10 | 2022-04-12 | 安徽医科大学 | 一种基于CRISPR/Cas系统的新型冠状病毒双靶标快速检测方法及试剂盒 |
| JPWO2023195542A1 (https=) * | 2022-04-08 | 2023-10-12 | ||
| WO2024072775A1 (en) * | 2022-09-26 | 2024-04-04 | The Johns Hopkins University | Devices and systems for dna capture |
| JPWO2024111543A1 (https=) * | 2022-11-25 | 2024-05-30 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3727026B2 (ja) | 2003-04-10 | 2005-12-14 | 博行 野地 | 一分子酵素活性検出に用いられるマイクロチャンバと１０００ｆＬ以下の液滴を調製する方法 |
| WO2008105814A2 (en) * | 2006-08-22 | 2008-09-04 | Los Alamos National Security, Llc | Miniturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids |
| EP3101115B1 (en) | 2014-01-31 | 2020-10-21 | Toppan Printing Co., Ltd. | Biomolecule analysis kit and biomolecule analysis method |
| US10337051B2 (en) * | 2016-06-16 | 2019-07-02 | The Regents Of The University Of California | Methods and compositions for detecting a target RNA |
| CN111479928A (zh) | 2017-11-17 | 2020-07-31 | 凸版印刷株式会社 | 靶分子的检测方法 |
| US10253365B1 (en) * | 2017-11-22 | 2019-04-09 | The Regents Of The University Of California | Type V CRISPR/Cas effector proteins for cleaving ssDNAs and detecting target DNAs |
| CN111836903A (zh) * | 2017-12-22 | 2020-10-27 | 博德研究所 | 基于crispr效应系统的多重诊断 |
| JP2019125564A (ja) | 2018-01-19 | 2019-07-25 | 株式会社豊田自動織機 | 蓄電モジュール |
| CA3119971A1 (en) | 2018-11-14 | 2020-05-22 | Pardis SABETI | Multiplexing highly evolving viral variants with sherlock |
| CN114072520A (zh) | 2019-07-04 | 2022-02-18 | 国立研究开发法人理化学研究所 | 检测靶核酸片段的方法及试剂盒 |
| US20240094199A1 (en) * | 2020-12-28 | 2024-03-21 | Riken | Method and kit for detecting target nucleic acid fragment |
-
2020
- 2020-07-06 CN CN202080047412.4A patent/CN114072520A/zh active Pending
- 2020-07-06 EP EP20834193.3A patent/EP3995833A4/en active Pending
- 2020-07-06 US US17/620,868 patent/US12522859B2/en active Active
- 2020-07-06 WO PCT/JP2020/026371 patent/WO2021002476A1/ja not_active Ceased
- 2020-07-06 CA CA3143923A patent/CA3143923A1/en active Pending
- 2020-07-06 JP JP2021529209A patent/JP7579577B2/ja active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20230279375A1 (en) * | 2021-12-13 | 2023-09-07 | Labsimply, Inc. | Signal boost cascade assay |
Also Published As
| Publication number | Publication date |
|---|---|
| US20220348993A1 (en) | 2022-11-03 |
| US12522859B2 (en) | 2026-01-13 |
| JP7579577B2 (ja) | 2024-11-08 |
| EP3995833A1 (en) | 2022-05-11 |
| CN114072520A (zh) | 2022-02-18 |
| JPWO2021002476A1 (https=) | 2021-01-07 |
| WO2021002476A1 (ja) | 2021-01-07 |
| EP3995833A4 (en) | 2023-08-09 |
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