CN112204146B - 具有受抑制的乙醇产生途径的耐酸酵母及使用其生产乳酸的方法 - Google Patents
具有受抑制的乙醇产生途径的耐酸酵母及使用其生产乳酸的方法 Download PDFInfo
- Publication number
- CN112204146B CN112204146B CN201980034827.5A CN201980034827A CN112204146B CN 112204146 B CN112204146 B CN 112204146B CN 201980034827 A CN201980034827 A CN 201980034827A CN 112204146 B CN112204146 B CN 112204146B
- Authority
- CN
- China
- Prior art keywords
- lactic acid
- acid
- gene
- ala
- strain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
- C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/56—Lactic acid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
- C12Y101/01027—L-Lactate dehydrogenase (1.1.1.27)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020180044509A KR102140597B1 (ko) | 2018-04-17 | 2018-04-17 | 에탄올 생산 경로가 억제된 내산성 효모 및 이를 이용한 젖산의 제조방법 |
| KR10-2018-0044509 | 2018-04-17 | ||
| PCT/KR2019/002433 WO2019203436A1 (ko) | 2018-04-17 | 2019-02-28 | 에탄올 생산 경로가 억제된 내산성 효모 및 이를 이용한 젖산의 제조방법 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112204146A CN112204146A (zh) | 2021-01-08 |
| CN112204146B true CN112204146B (zh) | 2024-10-22 |
Family
ID=68239697
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201980034827.5A Active CN112204146B (zh) | 2018-04-17 | 2019-02-28 | 具有受抑制的乙醇产生途径的耐酸酵母及使用其生产乳酸的方法 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US11339398B2 (https=) |
| EP (1) | EP3795689A4 (https=) |
| JP (1) | JP7395501B2 (https=) |
| KR (1) | KR102140597B1 (https=) |
| CN (1) | CN112204146B (https=) |
| WO (1) | WO2019203436A1 (https=) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102140596B1 (ko) | 2018-04-17 | 2020-08-04 | 에스케이이노베이션 주식회사 | 유기산 내성 효모 유래 신규 프로모터 및 이를 이용한 목적유전자의 발현방법 |
| KR102736070B1 (ko) | 2018-10-08 | 2024-12-02 | 에스케이이노베이션 주식회사 | 알코올 생성이 억제된 재조합 내산성 효모 및 이를 이용한 젖산의 제조방법 |
| KR20210041903A (ko) | 2019-10-08 | 2021-04-16 | 에스케이이노베이션 주식회사 | 락테이트 대사 및 알코올 생성이 억제된 재조합 내산성 효모 및 이를 이용한 젖산의 제조방법 |
| KR20210128742A (ko) * | 2020-04-17 | 2021-10-27 | 에스케이이노베이션 주식회사 | 글리세롤 생성이 억제된 재조합 내산성 효모 및 이를 이용한 젖산의 제조방법 |
| KR20210158676A (ko) | 2020-06-24 | 2021-12-31 | 에스케이이노베이션 주식회사 | 젖산 생산능이 증가된 재조합 내산성 효모 |
| KR20220064647A (ko) | 2020-11-12 | 2022-05-19 | 에스케이이노베이션 주식회사 | 내산성 효모 유전자 기반 합성 프로모터 |
| WO2023004336A1 (en) * | 2021-07-22 | 2023-01-26 | Archer Daniels Midland Company | A genetically engineered yeast producing lactic acid |
| CN118086080B (zh) * | 2024-04-11 | 2024-06-25 | 内蒙古工业大学 | 一株耐酸杰丁塞伯林德纳氏酵母及其应用 |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IT1294728B1 (it) * | 1997-09-12 | 1999-04-12 | Biopolo S C A R L | Ceppi di lievito per la riproduzione di acido lattico |
| JP2001204464A (ja) | 2000-01-27 | 2001-07-31 | Toyota Motor Corp | 乳酸の製造方法 |
| US7141410B2 (en) | 2000-11-22 | 2006-11-28 | Natureworks Llc | Methods and materials for the production of organic products in cells of Candida species |
| ES2529254T3 (es) | 2002-05-30 | 2015-02-18 | Cargill Inc. | Proceso de fermentación usando velocidades de incorporación de oxígeno específicas como un control del proceso |
| JP4095889B2 (ja) | 2002-12-13 | 2008-06-04 | トヨタ自動車株式会社 | 高光学純度な乳酸の製造方法 |
| JP4460876B2 (ja) * | 2003-11-07 | 2010-05-12 | 株式会社豊田中央研究所 | 有機酸存在下におけるプロモーター及びその利用 |
| JP2006006271A (ja) | 2004-06-29 | 2006-01-12 | Toyota Central Res & Dev Lab Inc | 乳酸生産酵母および乳酸生産方法 |
| JP4692173B2 (ja) | 2005-09-13 | 2011-06-01 | 東レ株式会社 | Ｄ−乳酸デヒドロゲナーゼ活性を有するポリペプチド、これをコードする遺伝子およびｄ−乳酸の製造方法 |
| WO2014030655A1 (ja) | 2012-08-24 | 2014-02-27 | 旭硝子株式会社 | 形質転換体およびその製造方法、ならびに乳酸の製造方法 |
| KR102155697B1 (ko) | 2013-02-05 | 2020-09-14 | 삼성전자주식회사 | 젖산트랜스포터 유전자가 과발현되고, 젖산 분해가 억제된 미생물 및 이를 이용한 젖산 생산 방법 |
| CN107709540B (zh) | 2014-06-20 | 2021-04-09 | 韩国生命工学研究院 | 库德里阿兹威氏毕赤酵母ng7微生物及其用途 |
| KR20160012561A (ko) * | 2014-07-24 | 2016-02-03 | 삼성전자주식회사 | Erg5의 활성이 증가되도록 유전적으로 조작된, 내산성을 갖는 효모 세포 및 그를 이용하여 락테이트를 생산하는 방법 |
| CN107002019B (zh) | 2014-08-29 | 2021-11-23 | Sk新技术株式会社 | 产生3-羟基丙酸的重组酵母和使用其产生3-羟基丙酸的方法 |
| KR102303832B1 (ko) * | 2015-05-12 | 2021-09-17 | 삼성전자주식회사 | 내산성을 갖는 효모 세포, 상기 효모 세포를 제조하는 방법 및 이의 용도 |
| KR101704212B1 (ko) | 2015-06-12 | 2017-02-08 | 씨제이제일제당 (주) | 젖산을 생산하는 미생물 및 이를 이용한 젖산 제조 방법 |
| KR102140596B1 (ko) | 2018-04-17 | 2020-08-04 | 에스케이이노베이션 주식회사 | 유기산 내성 효모 유래 신규 프로모터 및 이를 이용한 목적유전자의 발현방법 |
-
2018
- 2018-04-17 KR KR1020180044509A patent/KR102140597B1/ko active Active
-
2019
- 2019-02-28 JP JP2020557301A patent/JP7395501B2/ja active Active
- 2019-02-28 US US17/047,810 patent/US11339398B2/en active Active
- 2019-02-28 CN CN201980034827.5A patent/CN112204146B/zh active Active
- 2019-02-28 WO PCT/KR2019/002433 patent/WO2019203436A1/ko not_active Ceased
- 2019-02-28 EP EP19787994.3A patent/EP3795689A4/en active Pending
Non-Patent Citations (1)
| Title |
|---|
| Lactic acid production by Saccharomyces cerevisiae expressing a Rhizopus oryzae lactate dehydrogenase gene;CHRISTOPHER D. SKORY等;Lactic acid production by Saccharomyces cerevisiae expressing a Rhizopus oryzae lactate dehydrogenase gene;第30卷(第1期);第22-27页，尤其是摘要；第23页，比较例1；第25页，第2栏，最后一段；图5 * |
Also Published As
| Publication number | Publication date |
|---|---|
| KR20190121031A (ko) | 2019-10-25 |
| EP3795689A4 (en) | 2022-01-26 |
| US11339398B2 (en) | 2022-05-24 |
| JP7395501B2 (ja) | 2023-12-11 |
| JP2021520836A (ja) | 2021-08-26 |
| CN112204146A (zh) | 2021-01-08 |
| US20210155945A1 (en) | 2021-05-27 |
| EP3795689A1 (en) | 2021-03-24 |
| WO2019203436A1 (ko) | 2019-10-24 |
| KR102140597B1 (ko) | 2020-08-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112204146B (zh) | 具有受抑制的乙醇产生途径的耐酸酵母及使用其生产乳酸的方法 | |
| CN112725210B (zh) | 抑制乳酸代谢和乙醇产生的重组耐酸酵母及使用其生产乳酸的方法 | |
| CN112789353B (zh) | 抑制乙醇产生的重组耐酸酵母以及使用其制备乳酸的方法 | |
| CN113528362B (zh) | 甘油产生受到抑制的重组耐酸酵母和使用其生产乳酸的方法 | |
| CN104789638A (zh) | 酵母中生产乙酰-CoA的酶 | |
| JP2006525025A (ja) | ピルビン酸産生酵母株 | |
| CN107709540A (zh) | 新型库德里阿兹威氏毕赤酵母菌种ng7及其用途 | |
| EP3415628B1 (en) | Recombinant mutant microorganism having malonic acid production capability and method for producing malonic acid using same | |
| EP3380626B1 (en) | Genetically modified yeasts and fermentation processes using genetically modified yeasts | |
| RU2422526C2 (ru) | СПОСОБ ПОЛУЧЕНИЯ ЯНТАРНОЙ КИСЛОТЫ С ИСПОЛЬЗОВАНИЕМ ДРОЖЖЕЙ, ПРИНАДЛЕЖАЩИХ К РОДУ Yarrowia | |
| CN102016024A (zh) | 突变体酵母及使用其的物质生产方法 | |
| JP2022008224A (ja) | 乳酸生産能が増加した組換え耐酸性酵母 | |
| KR100869623B1 (ko) | 하이드록시 카복실산류의 생산 방법 | |
| WO2023246071A1 (zh) | 一种mreC突变体及其在L-缬氨酸发酵生产中的应用 | |
| CN118302514A (zh) | 一种高产l-苹果酸的耐酸酵母菌株及其构建方法和应用 | |
| KR102078715B1 (ko) | 젖산 생산이 향상된 형질전환된 미생물 | |
| KR102330595B1 (ko) | 에탄올 생산 경로가 억제된 내산성 효모 및 이를 이용한 젖산의 제조방법 | |
| WO2023168244A1 (en) | Genetically modified yeast and fermentation processes for the production of 3-hydroxypropionate | |
| CN106676120A (zh) | 一种提高产溶剂梭菌发酵性能的方法 | |
| KR102253701B1 (ko) | 하이브리드형 해당 경로 | |
| CN120858175A (zh) | 用于生产d-阿洛酮糖的遗传修饰的微生物和发酵工艺 | |
| AT526405A1 (de) | Synthetischer Formolaseweg | |
| ES2715930T3 (es) | Productores microbianos de ácido succínico novedosos y purificación del ácido succínico | |
| WO2016002680A1 (ja) | 形質転換体およびその製造方法、ならびに炭素数４のジカルボン酸の製造方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |