KR102140597B1 - 에탄올 생산 경로가 억제된 내산성 효모 및 이를 이용한 젖산의 제조방법 - Google Patents

에탄올 생산 경로가 억제된 내산성 효모 및 이를 이용한 젖산의 제조방법 Download PDF

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KR102140597B1
KR102140597B1 KR1020180044509A KR20180044509A KR102140597B1 KR 102140597 B1 KR102140597 B1 KR 102140597B1 KR 1020180044509 A KR1020180044509 A KR 1020180044509A KR 20180044509 A KR20180044509 A KR 20180044509A KR 102140597 B1 KR102140597 B1 KR 102140597B1
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gene
lactic acid
strain
ala
acid
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KR20190121031A (ko
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박재연
이태영
이기성
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에스케이이노베이션 주식회사
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Priority to CN201980034827.5A priority patent/CN112204146B/zh
Priority to JP2020557301A priority patent/JP7395501B2/ja
Priority to US17/047,810 priority patent/US11339398B2/en
Priority to PCT/KR2019/002433 priority patent/WO2019203436A1/ko
Priority to EP19787994.3A priority patent/EP3795689A4/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/56—Lactic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01027—L-Lactate dehydrogenase (1.1.1.27)

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  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
KR1020180044509A 2018-04-17 2018-04-17 에탄올 생산 경로가 억제된 내산성 효모 및 이를 이용한 젖산의 제조방법 Active KR102140597B1 (ko)

Priority Applications (7)

Application Number Priority Date Filing Date Title
KR1020180044509A KR102140597B1 (ko) 2018-04-17 2018-04-17 에탄올 생산 경로가 억제된 내산성 효모 및 이를 이용한 젖산의 제조방법
JP2020557301A JP7395501B2 (ja) 2018-04-17 2019-02-28 エタノール生産経路が抑制された耐酸性酵母及びこれを用いた乳酸の製造方法
US17/047,810 US11339398B2 (en) 2018-04-17 2019-02-28 Acid-resistant yeast with suppressed ethanol production pathway and method for producing lactic acid using same
PCT/KR2019/002433 WO2019203436A1 (ko) 2018-04-17 2019-02-28 에탄올 생산 경로가 억제된 내산성 효모 및 이를 이용한 젖산의 제조방법
CN201980034827.5A CN112204146B (zh) 2018-04-17 2019-02-28 具有受抑制的乙醇产生途径的耐酸酵母及使用其生产乳酸的方法
EP19787994.3A EP3795689A4 (en) 2018-04-17 2019-02-28 ACID-RESISTANT YEAST WITH SUPPRESSED ETHANOL PRODUCTION PATHWAY AND PROCESS FOR THE PRODUCTION OF LACTIC ACID USING THEM
KR1020200093598A KR102330595B1 (ko) 2018-04-17 2020-07-28 에탄올 생산 경로가 억제된 내산성 효모 및 이를 이용한 젖산의 제조방법

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KR1020180044509A KR102140597B1 (ko) 2018-04-17 2018-04-17 에탄올 생산 경로가 억제된 내산성 효모 및 이를 이용한 젖산의 제조방법

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KR1020200093598A Division KR102330595B1 (ko) 2018-04-17 2020-07-28 에탄올 생산 경로가 억제된 내산성 효모 및 이를 이용한 젖산의 제조방법

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KR20190121031A KR20190121031A (ko) 2019-10-25
KR102140597B1 true KR102140597B1 (ko) 2020-08-03

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US (1) US11339398B2 (https=)
EP (1) EP3795689A4 (https=)
JP (1) JP7395501B2 (https=)
KR (1) KR102140597B1 (https=)
CN (1) CN112204146B (https=)
WO (1) WO2019203436A1 (https=)

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Publication number Priority date Publication date Assignee Title
EP4008771A1 (en) 2020-11-12 2022-06-08 SK Innovation Co., Ltd. Synthetic promoter based on gene from acid-resistant yeast

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KR102140596B1 (ko) 2018-04-17 2020-08-04 에스케이이노베이션 주식회사 유기산 내성 효모 유래 신규 프로모터 및 이를 이용한 목적유전자의 발현방법
KR102736070B1 (ko) 2018-10-08 2024-12-02 에스케이이노베이션 주식회사 알코올 생성이 억제된 재조합 내산성 효모 및 이를 이용한 젖산의 제조방법
KR20210041903A (ko) 2019-10-08 2021-04-16 에스케이이노베이션 주식회사 락테이트 대사 및 알코올 생성이 억제된 재조합 내산성 효모 및 이를 이용한 젖산의 제조방법
KR20210128742A (ko) * 2020-04-17 2021-10-27 에스케이이노베이션 주식회사 글리세롤 생성이 억제된 재조합 내산성 효모 및 이를 이용한 젖산의 제조방법
KR20210158676A (ko) 2020-06-24 2021-12-31 에스케이이노베이션 주식회사 젖산 생산능이 증가된 재조합 내산성 효모
WO2023004336A1 (en) * 2021-07-22 2023-01-26 Archer Daniels Midland Company A genetically engineered yeast producing lactic acid
CN118086080B (zh) * 2024-04-11 2024-06-25 内蒙古工业大学 一株耐酸杰丁塞伯林德纳氏酵母及其应用

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Cited By (2)

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Publication number Priority date Publication date Assignee Title
EP4008771A1 (en) 2020-11-12 2022-06-08 SK Innovation Co., Ltd. Synthetic promoter based on gene from acid-resistant yeast
US11788095B2 (en) 2020-11-12 2023-10-17 Sk Innovation Co., Ltd. Synthetic promoter based on gene from acid-resistant yeast

Also Published As

Publication number Publication date
KR20190121031A (ko) 2019-10-25
EP3795689A4 (en) 2022-01-26
US11339398B2 (en) 2022-05-24
JP7395501B2 (ja) 2023-12-11
JP2021520836A (ja) 2021-08-26
CN112204146B (zh) 2024-10-22
CN112204146A (zh) 2021-01-08
US20210155945A1 (en) 2021-05-27
EP3795689A1 (en) 2021-03-24
WO2019203436A1 (ko) 2019-10-24

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