JP7395501B2 - エタノール生産経路が抑制された耐酸性酵母及びこれを用いた乳酸の製造方法 - Google Patents

エタノール生産経路が抑制された耐酸性酵母及びこれを用いた乳酸の製造方法 Download PDF

Info

Publication number
JP7395501B2
JP7395501B2 JP2020557301A JP2020557301A JP7395501B2 JP 7395501 B2 JP7395501 B2 JP 7395501B2 JP 2020557301 A JP2020557301 A JP 2020557301A JP 2020557301 A JP2020557301 A JP 2020557301A JP 7395501 B2 JP7395501 B2 JP 7395501B2
Authority
JP
Japan
Prior art keywords
gene
lactic acid
strain
acid
seq
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2020557301A
Other languages
English (en)
Japanese (ja)
Other versions
JP2021520836A5 (https=
JP2021520836A (ja
Inventor
パク チェ・ヨン
リー テ・ヨン
リー キ・スン
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SK Innovation Co Ltd
Original Assignee
SK Innovation Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SK Innovation Co Ltd filed Critical SK Innovation Co Ltd
Publication of JP2021520836A publication Critical patent/JP2021520836A/ja
Publication of JP2021520836A5 publication Critical patent/JP2021520836A5/ja
Application granted granted Critical
Publication of JP7395501B2 publication Critical patent/JP7395501B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/56—Lactic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01027—L-Lactate dehydrogenase (1.1.1.27)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2020557301A 2018-04-17 2019-02-28 エタノール生産経路が抑制された耐酸性酵母及びこれを用いた乳酸の製造方法 Active JP7395501B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR1020180044509A KR102140597B1 (ko) 2018-04-17 2018-04-17 에탄올 생산 경로가 억제된 내산성 효모 및 이를 이용한 젖산의 제조방법
KR10-2018-0044509 2018-04-17
PCT/KR2019/002433 WO2019203436A1 (ko) 2018-04-17 2019-02-28 에탄올 생산 경로가 억제된 내산성 효모 및 이를 이용한 젖산의 제조방법

Publications (3)

Publication Number Publication Date
JP2021520836A JP2021520836A (ja) 2021-08-26
JP2021520836A5 JP2021520836A5 (https=) 2021-10-07
JP7395501B2 true JP7395501B2 (ja) 2023-12-11

Family

ID=68239697

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2020557301A Active JP7395501B2 (ja) 2018-04-17 2019-02-28 エタノール生産経路が抑制された耐酸性酵母及びこれを用いた乳酸の製造方法

Country Status (6)

Country Link
US (1) US11339398B2 (https=)
EP (1) EP3795689A4 (https=)
JP (1) JP7395501B2 (https=)
KR (1) KR102140597B1 (https=)
CN (1) CN112204146B (https=)
WO (1) WO2019203436A1 (https=)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102140596B1 (ko) 2018-04-17 2020-08-04 에스케이이노베이션 주식회사 유기산 내성 효모 유래 신규 프로모터 및 이를 이용한 목적유전자의 발현방법
KR102736070B1 (ko) 2018-10-08 2024-12-02 에스케이이노베이션 주식회사 알코올 생성이 억제된 재조합 내산성 효모 및 이를 이용한 젖산의 제조방법
KR20210041903A (ko) 2019-10-08 2021-04-16 에스케이이노베이션 주식회사 락테이트 대사 및 알코올 생성이 억제된 재조합 내산성 효모 및 이를 이용한 젖산의 제조방법
KR20210128742A (ko) * 2020-04-17 2021-10-27 에스케이이노베이션 주식회사 글리세롤 생성이 억제된 재조합 내산성 효모 및 이를 이용한 젖산의 제조방법
KR20210158676A (ko) 2020-06-24 2021-12-31 에스케이이노베이션 주식회사 젖산 생산능이 증가된 재조합 내산성 효모
KR20220064647A (ko) 2020-11-12 2022-05-19 에스케이이노베이션 주식회사 내산성 효모 유전자 기반 합성 프로모터
WO2023004336A1 (en) * 2021-07-22 2023-01-26 Archer Daniels Midland Company A genetically engineered yeast producing lactic acid
CN118086080B (zh) * 2024-04-11 2024-06-25 内蒙古工业大学 一株耐酸杰丁塞伯林德纳氏酵母及其应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006006271A (ja) 2004-06-29 2006-01-12 Toyota Central Res & Dev Lab Inc 乳酸生産酵母および乳酸生産方法
WO2014030655A1 (ja) 2012-08-24 2014-02-27 旭硝子株式会社 形質転換体およびその製造方法、ならびに乳酸の製造方法
WO2016200207A1 (ko) 2015-06-12 2016-12-15 씨제이제일제당 (주) 젖산을 생산하는 미생물 및 이를 이용한 젖산 제조 방법
JP2017525372A (ja) 2014-08-29 2017-09-07 エスケー イノベーション カンパニー リミテッドＳｋ Ｉｎｎｏｖａｔｉｏｎ Ｃｏ．，Ｌｔｄ． ３−ｈｐを生産することができる組換え酵母およびこれを利用した３−ｈｐの製造方法

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1294728B1 (it) * 1997-09-12 1999-04-12 Biopolo S C A R L Ceppi di lievito per la riproduzione di acido lattico
JP2001204464A (ja) 2000-01-27 2001-07-31 Toyota Motor Corp 乳酸の製造方法
US7141410B2 (en) 2000-11-22 2006-11-28 Natureworks Llc Methods and materials for the production of organic products in cells of Candida species
ES2529254T3 (es) 2002-05-30 2015-02-18 Cargill Inc. Proceso de fermentación usando velocidades de incorporación de oxígeno específicas como un control del proceso
JP4095889B2 (ja) 2002-12-13 2008-06-04 トヨタ自動車株式会社 高光学純度な乳酸の製造方法
JP4460876B2 (ja) * 2003-11-07 2010-05-12 株式会社豊田中央研究所 有機酸存在下におけるプロモーター及びその利用
JP4692173B2 (ja) 2005-09-13 2011-06-01 東レ株式会社 Ｄ−乳酸デヒドロゲナーゼ活性を有するポリペプチド、これをコードする遺伝子およびｄ−乳酸の製造方法
KR102155697B1 (ko) 2013-02-05 2020-09-14 삼성전자주식회사 젖산트랜스포터 유전자가 과발현되고, 젖산 분해가 억제된 미생물 및 이를 이용한 젖산 생산 방법
CN107709540B (zh) 2014-06-20 2021-04-09 韩国生命工学研究院 库德里阿兹威氏毕赤酵母ng7微生物及其用途
KR20160012561A (ko) * 2014-07-24 2016-02-03 삼성전자주식회사 Erg5의 활성이 증가되도록 유전적으로 조작된, 내산성을 갖는 효모 세포 및 그를 이용하여 락테이트를 생산하는 방법
KR102303832B1 (ko) * 2015-05-12 2021-09-17 삼성전자주식회사 내산성을 갖는 효모 세포, 상기 효모 세포를 제조하는 방법 및 이의 용도
KR102140596B1 (ko) 2018-04-17 2020-08-04 에스케이이노베이션 주식회사 유기산 내성 효모 유래 신규 프로모터 및 이를 이용한 목적유전자의 발현방법

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006006271A (ja) 2004-06-29 2006-01-12 Toyota Central Res & Dev Lab Inc 乳酸生産酵母および乳酸生産方法
WO2014030655A1 (ja) 2012-08-24 2014-02-27 旭硝子株式会社 形質転換体およびその製造方法、ならびに乳酸の製造方法
JP2017525372A (ja) 2014-08-29 2017-09-07 エスケー イノベーション カンパニー リミテッドＳｋ Ｉｎｎｏｖａｔｉｏｎ Ｃｏ．，Ｌｔｄ． ３−ｈｐを生産することができる組換え酵母およびこれを利用した３−ｈｐの製造方法
WO2016200207A1 (ko) 2015-06-12 2016-12-15 씨제이제일제당 (주) 젖산을 생산하는 미생물 및 이를 이용한 젖산 제조 방법

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Christopher D. Skory，Lactic acid production by Saccharomyces cerevisiae expressing a Rhizopus oryzae lactate dehydrogenase gene，J Ind Microbiol Biotechnol，2003年，30，22-27

Also Published As

Publication number Publication date
KR20190121031A (ko) 2019-10-25
EP3795689A4 (en) 2022-01-26
US11339398B2 (en) 2022-05-24
JP2021520836A (ja) 2021-08-26
CN112204146B (zh) 2024-10-22
CN112204146A (zh) 2021-01-08
US20210155945A1 (en) 2021-05-27
EP3795689A1 (en) 2021-03-24
WO2019203436A1 (ko) 2019-10-24
KR102140597B1 (ko) 2020-08-03

Similar Documents

Publication Publication Date Title
JP7395501B2 (ja) エタノール生産経路が抑制された耐酸性酵母及びこれを用いた乳酸の製造方法
JP7304889B2 (ja) 有機酸耐性酵母由来新規プロモーター及びこれを用いた目的遺伝子の発現方法
US8945888B2 (en) Method for producing high amount of glycolic acid by fermentation
JP7644581B2 (ja) 乳酸代謝及びアルコール生成の抑制された組み換え耐酸性酵母及びこれを用いた乳酸の製造方法
US11898173B2 (en) Recombinant acid-resistant yeast having improved lactic-acid-producing ability
US20120244588A1 (en) Method of producing 3-hydroxypropionic acid using malonic semialdehyde reducing pathway
JP7530891B2 (ja) アルコール生成が抑制された組換え耐酸性酵母及びこれを用いた乳酸の製造方法
JP7829285B2 (ja) グリセロール生成が抑制された組換え耐酸性酵母及びこれを用いた乳酸の製造方法
JP2012506716A (ja) グリセロールを化学物質に変換するための微好気性培養
US20130210097A1 (en) Glycolic acid fermentative production with a modified microorganism
JP5243546B2 (ja) 植物由来原料から乳酸を生産する方法及び乳酸生産細菌
MX2015004180A (es) Microorganismos recombinantes para producir acidos organicos organicos.
JP4473219B2 (ja) Ｄ−乳酸生産用生体触媒
EP4558636A1 (en) Deletion of the nde1 gene for improved lactic acid production in a genetically engineered yeast
CN100560727C (zh) 用于生产d-乳酸的生物催化剂
US11788095B2 (en) Synthetic promoter based on gene from acid-resistant yeast
JP2005102625A (ja) Ｄ−乳酸製造法
KR102330595B1 (ko) 에탄올 생산 경로가 억제된 내산성 효모 및 이를 이용한 젖산의 제조방법
JP5858463B2 (ja) 乳酸生産菌
HK1211318A1 (en) Materials and methods for efficient succinate and malate production

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20201210

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20210709

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20220127

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20221215

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20230117

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20230417

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20230627

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20230926

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20231107

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20231129

R150 Certificate of patent or registration of utility model

Ref document number: 7395501

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150