CN110859130A - Method for rooting tissue culture seedlings of medicinal plant of cornua cervi pantotrichum outside bottle - Google Patents
Method for rooting tissue culture seedlings of medicinal plant of cornua cervi pantotrichum outside bottle Download PDFInfo
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Abstract
The invention belongs to the technical field of tissue culture of herbaceous medicinal plants, and particularly relates to an ex-bottle rooting method for a tissue culture seedling of antlerpilose grass, which mainly solves the technical problems of long rooting period in the tissue culture seedling bottle, more induced aerial roots, low transplanting survival rate and the like, and comprises the following steps: (1) selecting bottle seedlings; (2) strong seedling culture; (3) hardening seedlings; (4) transplanting tissue culture seedlings; (5) after-planting management, the invention establishes an effective ecto-bottle rooting technology for the tissue culture seedlings of the savatier monochasma herb and optimizes a tissue culture and rapid propagation system of the tissue culture seedlings. The technology does not need to take root in a bottle, and the seedlings are directly transplanted after hormone treatment, after two weeks, new roots grow out from the base parts of the seedlings, after 30 days, the number of the roots grows to 5-10, the roots grow to 0.5-1.5 cm, and the robust hairy antler grass rooted seedlings with the seedling height of about 6-10 cm can be obtained in about 2 months. Compared with the traditional bottle rooting, the seedling culture period is shortened by one month, the transplanting survival rate is higher than 85 percent, the quality of the rooting seedlings is obviously improved by adopting the technology of the invention, and the industrialized production can be carried out.
Description
Technical Field
The invention relates to the technical field of the tissue culture of a pantoea plant, in particular to an ex-bottle rooting method for a medicinal plant pantoea tissue culture seedling.
Background
The pilose antler grass is a small perennial semi-parasitic herb plant of pilose antler grass of Scrophulariaceae, and is one of the main components of Chinese medicinal protective varieties, namely Yanning granules. Mainly distributed in the places of southeast China, such as Jiangxi, Hunan, Fujian, Zhejiang and Jiangsu, and is grown in the mountain slope and sunny weeds or under the Pinus massoniana forest. The literature reports that the velvet grass contains various secondary metabolites, and the separated phenylethanoid glycosides and flavonoids have certain anticomplementary activity, have the effects of bacteriostasis, anti-inflammation, antivirus and the like, are clinically used for treating diseases such as cold, cough, pneumonia, toothache and the like, and are important medicinal plants in forests. At present, the market of the cornua cervi pantotrichum mainly depends on wild resources, but the cornua cervi pantotrichum is excessively harvested and dug in an artificial extinction mode along with serious damage of a living environment, and the natural updating capability of the cornua cervi pantotrichum is extremely poor, so that the wild resources of the cornua cervi pantotrichum are gradually exhausted.
The research reports of the pantoea herb at home and abroad mainly focus on medicinal components and efficacies thereof, but the research on the cultivation and propagation of the pantoea herb is less, and only reports of seed germination and transplanting methods are found (CN 102498781A; CN 109757313A). The research and practice on the aspect of tissue culture of the antlerpilose grass shows that the tissue culture and rapid propagation technology is still immature, and a large amount of aerial roots distributed on the surface of a culture medium are generated by induction in a bottle, so far, the report of transplantation survival is not seen. The in-bottle rooting and transplanting domestication of the tissue culture seedling are combined by the out-of-bottle rooting technology, so that the production process of the tissue culture seedling is simplified, the space and the culture period of a culture room are saved, the production cost is reduced, and the production efficiency is improved.
Disclosure of Invention
The invention aims to solve the problems that: provides a rapid ex vitro rooting method for the tissue culture seedlings of the savatier monochasma.
The technical problem to be solved by the invention is as follows: provides a rapid ex vitro rooting method for a tissue culture seedling of antlerpilose grass, which comprises the following steps:
(1) selecting bottle seedlings: selecting hairy antler grass tissue culture seedling cluster buds (figure 1) with good growth vigor, soft leaves and silvery white color for strong seedling culture;
(2) strong seedling culture: cutting off multiple buds of the tissue culture seedling in a single plant, selecting single buds with terminal buds and with the height of about 2cm, transferring the single buds to a strong seedling culture medium, culturing for 20 days, and hardening the seedling when the height of the seedling is about 3-5 cm;
(3) hardening seedlings: firstly, putting the tissue culture seedlings of the savatier monochasma into a seedling hardening room with a sunshade net, controlling the temperature at 25-28 ℃, culturing for about one week, opening a bottle cap, and continuously culturing for 2-3 days.
(4) Matrix preparation and treatment: subpackaging the mixed and prepared transplanting matrix into seedling culture containers, thoroughly watering the seedling culture containers, and disinfecting the matrix by using potassium permanganate and carbendazim;
(5) rooting outside the tissue culture seedling bottle: taking out the seedlings from the bottle, washing the culture medium at the base part of the plants with tap water, soaking the seedlings in rooting water before cuttage, wherein the cuttage depth is preferably within 0.5-1.0 cm, and covering a thin film arched shed;
(6) managing after planting: after 2 weeks, new roots are found at the base parts, the film can be lifted off at the moment, the number of the roots reaches 5-10 after 30 days, the root length is 0.5-1.5 cm, foliar fertilizer and bactericide are regularly sprayed, robust pilose antler grass seedlings with the seedling height of 6-10 cm and the root length of 2.0-4.0 cm can be obtained after about 2 months, and 3-5 new buds sprout at the base parts in autumn in the current year.
Preferably, the medium for strong seedlings in the step (2) contains the following components:
macroelements: KNO3:1200mg/L;NH4NO3:1650mg/L;Ca(NO3)2·4H2O:400mg/L; KH2PO4:170mg/L;MgSO4·7H2O:370mg/L;CaCl2·7H2O:240mg/L;
Organic components: inositol: 200 mg/L; glycine: 2 mg/L; thiamine hydrochloride: 0.4 mg/L; pyridoxine hydrochloride: 0.5 mg/L; nicotinic acid: 0.5 mg/L; the other components are the same as MS.
The formula of the culture medium is as follows: improved MS + BA0.3mg/L + IAA 0.5-1.0 mg/L + GA 0.2-0.4 mg/L + sucrose 30g/L + agar 1.8-2.0 g/L
Preferably, the illumination intensity in the seedling hardening room in the step (3) is enhanced to about 3000Lux from 1800-2000 Lux in the culture room, and the humidity is controlled to be 60-80%;
preferably, the formula of the substrate in the step (4) is 20-30% of peat, 20% of decomposed bark, 10-20% of carbonized chaff and 30% of yellow soil (by volume ratio), and all the components are fully and uniformly mixed according to the proportion;
preferably, in the step (4), a mixed solution of 0.5% of potassium permanganate and 0.08% of carbendazim solution in mass concentration is sprinkled by a sprinkling can for matrix disinfection, and the disinfected film is covered for more than 10 hours;
preferably, the rooting solution is prepared in the step (5): the mixed solution of 500ppm ABT and 1000ppm NAA, the time for soaking the rooting agent is 30-60 s, and the base part of the tissue culture seedling is soaked for 0.5-1.0 cm;
preferably, the foliar fertilizer in the step (6) is prepared by alternately using a large amount of urea and MS solution and spraying the solution once every 3 days;
preferably, the bactericide in the step (6) comprises carbendazim, thiophanate methyl, boldo liquid and chlorothalonil, and one or more of the bactericide is mixed and used alternately, and the concentration is preferably 1000-1500 ppm.
By adopting the technical scheme, the invention has the beneficial effects that:
the culture period of the tissue culture seedling is shortened, the culture steps are simplified, for example, the tissue culture seedling cultured by the method is subjected to in-bottle rooting culture, and the problems that roots generated by induction of a culture medium in a bottle do not extend into the culture medium, so that aerial roots are formed, and the transplanting is unsuccessful are solved;
secondly, the cost is low, the materials are convenient to obtain, and the operation is simple. The yellow core soil, peat and agricultural and forestry wastes (bark, sawdust, chaff and the like) are adopted, so that the wastes are recycled, the environment is protected, the cost is reduced, and the industrial production of seedlings is facilitated;
and thirdly, the survival rate of the tissue culture seedlings is improved. By the ex-vitro rooting technology, the transplanting survival rate of the method reaches more than 85 percent.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the technical descriptions of the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts.
FIG. 1 is a diagram of multiple shoots of tissue culture seedlings.
FIG. 2 is a diagram of tissue culture seedling strengthening.
FIG. 3 is a diagram before transplantation of tissue culture seedlings.
FIG. 4 is a diagram after transplantation of the tissue culture seedlings.
FIG. 5 is a graph of extra-bottle roots 50 d.
FIG. 6 is a graph of extra-bottle roots 50 d.
FIG. 7 is a conventional culture diagram.
FIG. 8 is a diagram showing the presence of aerial roots in conventional culture.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without creative efforts, shall fall within the protection scope of the present invention.
As shown in figures 1-6, a rapid ex vitro rooting method for a tissue culture seedling of pantoea, the technical scheme comprises the following steps:
(1) selecting bottle seedlings: selecting hairy antler grass tissue culture seedling cluster buds (figure 1) with good growth vigor, soft leaves and silvery white color for strong seedling culture;
(2) strong seedling culture: cutting off multiple buds of the tissue culture seedling in a single plant, selecting single buds with terminal buds and with the height of about 2cm, transferring the single buds to a strong seedling culture medium, culturing for 20 days, and hardening the seedling when the height of the seedling is about 3-5 cm;
(3) hardening seedlings: firstly, putting the tissue culture seedlings of the savatier monochasma into a seedling hardening room with a sunshade net, controlling the temperature at 25-28 ℃, culturing for about one week, opening a bottle cap, and continuously culturing for 2-3 days.
(4) Matrix preparation and treatment: subpackaging the mixed and prepared transplanting matrix into seedling culture containers, thoroughly watering the seedling culture containers, and disinfecting the matrix by using potassium permanganate and carbendazim;
(5) rooting outside the tissue culture seedling bottle: taking out the seedlings from the bottle, washing the culture medium at the base part of the plants with tap water, soaking the seedlings in rooting water before cuttage, wherein the cuttage depth is preferably within 0.5-1.0 cm, and covering a thin film arched shed;
(6) managing after planting: after 2 weeks, new roots are found at the base parts, the film can be lifted off at the moment, the number of the roots reaches 5-10 after 30 days, the root length is 0.5-1.5 cm, foliar fertilizer and bactericide are regularly sprayed, robust pilose antler grass seedlings with the seedling height of 6-10 cm and the root length of 2.0-4.0 cm can be obtained after about 2 months, and 3-5 new buds sprout at the base parts in autumn in the current year.
Preferably, the medium for strong seedlings in the step (2) contains the following components:
macroelements: KNO3:1200mg/L;NH4NO3:1650mg/L;Ca(NO3)2·4H2O:400mg/L; KH2PO4:170mg/L;MgSO4·7H2O:370mg/L;CaCl2·7H2O:240mg/L;
Organic components: inositol: 200 mg/L; glycine: 2 mg/L; thiamine hydrochloride: 0.4 mg/L; pyridoxine hydrochloride: 0.5 mg/L; nicotinic acid: 0.5 mg/L; the other components are the same as MS.
The formula of the culture medium is as follows: improved MS + BA0.3mg/L + IAA 0.5-1.0 mg/L + GA 0.2-0.4 mg/L + sucrose 30g/L + agar 1.8-2.0 g/L.
In the step (3), the illumination intensity in the seedling hardening room is enhanced to about 3000Lux from 1800-2000 Lux in the culture room, and the humidity is controlled to be 60-80%;
the formula of the substrate in the step (4) is 20-30% of peat, 20% of decomposed bark, 10-20% of carbonized chaff and 30% of yellow soil (by volume ratio), and all the components are fully and uniformly mixed according to the proportion;
spraying a mixed solution of 0.5 percent of potassium permanganate and 0.08 percent of carbendazim solution by using a spray can for matrix disinfection, and covering a film for more than 10 hours after disinfection;
preparing a rooting solution in the step (5): the mixed solution of 500ppm ABT and 1000ppm NAA, the time for soaking the rooting agent is 30-60 s, and the base part of the tissue culture seedling is soaked for 0.5-1.0 cm;
the foliar fertilizer in the step (6) is prepared by alternately using a large amount of urea and MS solutions and spraying the solutions once every 3 days;
the bactericide in the step (6) comprises carbendazim, thiophanate methyl, Bordeaux mixture and chlorothalonil, and one or more of the bactericide is mixed and used alternately, and the concentration is preferably 1000-1500 ppm.
Example 1:
(1) selecting bottle seedlings: selecting hairy antler grass tissue culture seedling cluster buds (figure 1) with good growth vigor, soft leaves and silvery white color for strong seedling culture;
(2) strong seedling culture: cutting off multiple buds of the tissue culture seedling in a single plant, selecting a single bud with a top bud and a seedling height of 2cm, and transferring the single bud to a strong seedling culture medium, wherein the strong seedling culture medium contains the following components: macroelements: KNO3:1200mg/L;NH4NO3:1650mg/L;Ca(NO3)2·4H2O:400mg/L; KH2PO4:170mg/L;MgSO4·7H2O:370mg/L;CaCl2·7H2O:240mg/L;
Organic components: inositol: 200 mg/L; glycine: 2 mg/L; thiamine hydrochloride: 0.4 mg/L; pyridoxine hydrochloride: 0.5 mg/L; nicotinic acid: 0.5 mg/L; the other components are the same as MS;
the formula of the culture medium is as follows: improving MS, BA0.3mg/L, IAA0.5mg/L, GA0.2mg/L, cane sugar 30g/L and jerusalem artichoke 1.8g/L, culturing for 20d, and hardening when the height of the seedlings is about 3 cm;
(3) hardening seedlings: firstly, putting the tissue culture seedlings of the antlerpilose grass into a seedling hardening chamber with a sunshade net, increasing the illumination intensity from 1800Lux in a culture room to 3000Lux in the seedling hardening chamber, controlling the humidity at 60 percent and the temperature at 25 ℃, culturing for about one week, opening a bottle cap, and continuously culturing for 2 days.
(4) Matrix preparation and treatment: the matrix formula is 20% of peat, 20% of decomposed bark, 10% of carbonized chaff and 30% of yellow soil (by volume ratio), all the components are fully and uniformly mixed according to the proportion, the mixed and prepared transplanting matrix is subpackaged into seedling culture containers, the seedling culture containers are watered thoroughly, the matrix is disinfected by sprinkling a mixed solution of 0.5% of potassium permanganate and 0.08% of carbendazim solution by a sprinkling can, and the disinfected film covers for more than 10 hours;
(5) rooting outside the tissue culture seedling bottle: taking out the seedling from the bottle, washing the culture medium at the base part of the plant with tap water, soaking the seedling with rooting water before cutting, preparing a mixed solution of 500ppm ABT and 1000ppm NAA with the rooting solution, soaking the rooting agent for 30s, soaking the base part of the tissue culture seedling for 0.5cm, properly controlling the cutting depth within 0.5cm, and covering a film arched shed;
(6) managing after planting: after 2 weeks, new roots are seen at the base part, the film can be lifted off at the moment, the number of roots reaches 5 after 30 days, the root length is 0.5-1.5 cm, a large amount of urea and MS solution is sprayed on the leaf fertilizer and the bactericide is sprayed on the leaf fertilizer regularly, the bactericide is sprayed once every 3 days, the bactericide comprises carbendazim, thiophanate methyl, Bordeaux mixture and chlorothalonil, the concentration is preferably 1000ppm, and robust pilose antler grass seedlings with the seedling height of about 8cm can be obtained in about 2 months.
Example 2:
(1) selecting bottle seedlings: selecting hairy antler grass tissue culture seedling cluster buds (figure 1) with good growth vigor, soft leaves and silvery white color for strong seedling culture;
(2) strong seedling culture: cutting off multiple buds of the tissue culture seedling in a single plant, selecting a single bud with a top bud and a seedling height of 2cm, and transferring the single bud to a strong seedling culture medium, wherein the strong seedling culture medium contains the following components: macroelements: KNO3:1200mg/L;NH4NO3:1650mg/L;Ca(NO3)2·4H2O:400mg/L; KH2PO4:170mg/L;MgSO4·7H2O:370mg/L;CaCl2·7H2O:240mg/L;
Organic components: inositol: 200 mg/L; glycine: 2 mg/L; thiamine hydrochloride: 0.4 mg/L; pyridoxine hydrochloride: 0.5 mg/L; nicotinic acid: 0.5 mg/L; the other components are the same as MS;
the formula of the culture medium is as follows: improving MS + BA0.3mg/L + IAA1.0mg/L + GA0.4mg/L + cane sugar 30g/L + jerusalem artichoke 2.0g/L, culturing for 20d, and hardening when the height of the seedlings is about 5cm (figure 2);
(3) hardening seedlings: firstly, putting the tissue culture seedlings of the velvet grass in a seedling hardening room with a sunshade net, increasing the illumination intensity from 2000Lux in a culture room to 3000Lux in the seedling hardening room, controlling the humidity at 80 percent and the temperature at 28 ℃, culturing for about one week, opening a bottle cap, and continuously culturing for 3 days.
(4) Matrix preparation and treatment: the matrix formula is 20% of peat, 20% of decomposed bark, 10% of carbonized chaff and 30% of yellow soil (by volume ratio), all the components are fully and uniformly mixed according to the proportion, the mixed and prepared transplanting matrix is subpackaged into seedling culture containers, the seedling culture containers are watered thoroughly, the matrix is disinfected by sprinkling a mixed solution of 0.5% of potassium permanganate and 0.08% of carbendazim solution by a sprinkling can, and the disinfected film covers for more than 10 hours;
(5) rooting outside the tissue culture seedling bottle: taking out the seedling from the bottle, washing the culture medium at the base of the plant with tap water (figure 3), soaking with rooting water before cutting, preparing a mixed solution of 500ppm ABT and 1000ppm NAA with rooting solution, soaking the rooting agent for 60s, soaking the base of the tissue culture seedling for 0.2cm, preferably within 0.2cm, and covering with a film arched shed;
(6) managing after planting: after 2 weeks, new roots are seen at the base part, the film can be lifted off at the moment, the number of roots reaches 10 after 30 days, the root length is 1.5cm, a foliar fertilizer and a bactericide are regularly sprayed, the foliar fertilizer is a large amount of urea and MS solution and is alternately used, the bactericide is sprayed once every 3 days, the bactericide comprises carbendazim, thiophanate methyl and bordeaux solution, the concentration is preferably 1500ppm, and robust pilose antler grass seedlings with the seedling height of about 10cm can be obtained after about 2 months (fig. 5 and 6).
Based on the 2 groups of specific embodiments, the robust velvet grass seedlings are cultured as shown in fig. 5 and 6, compared with the traditional in-bottle culture, the traditional cultured seedlings are thin and weak (as shown in fig. 8), the base parts are easy to callus, a large number of aerial roots are induced on the callus base parts or leaves, after the strong seedling culture, the subculture tissue culture seedlings grow rapidly, and the seedlings can grow from 1cm to 4-5 cm after being cultured for about 20 days for hardening and transplanting.
The application is based on different hormone treatments and is used for a test for transplanting the tissue culture seedlings of the antleria pilosa. 5 compositional activity rates and growth are reported in the table:
as can be seen from the table, when the hormone types and concentrations adopt BA0.2 and ABT0.5g/L, the survival rate is the highest and is 85.4 percent, and the growth condition in 4 months can reach the conditions that the stem and the leaf grow robustly, the plant height is 15-20cm, and the base part or the stem sprouts.
The applicant gives 5 groups of culture tests again, compares the steps of the invention one by one, analyzes the significance and progress brought by the steps of the invention, records and summarizes the following data table:
as can be seen from the table, in the first group, the rapid ex vitro rooting of the tissue culture seedlings of the velvet grass is completely carried out according to the steps of the invention, the survival time of 30 days can reach 88.9 percent, and the growth condition of 4 months can realize the robust growth of stems and leaves, the plant height is 15-20cm, and the base or stem sprouts (figure 7).
In the second group, due to the lack of the step of film covering, although the strong growth of stems and leaves can be realized in 4 months of growth, the plant height is 15-20cm, and new buds sprout on the base or the stem, the survival rate of 30d is only 33.3%.
The third group lacks the step of hardening seedlings, the strong growth of stems and leaves can be realized in 4 months of growth, the plant height is 15-20cm, and the base or stem sprouts, but the survival rate after 30d is 82.9 percent, which is lower than that of the first group.
The fourth group lacks two steps of hardening seedlings and covering the film, so that the survival rate of all deaths is only 2.4%.
In the fifth group, two steps of strengthening and hardening seedlings are lacked, the survival rate is 21.6% after 30d, the seedling preservation rate is lower than 50% in the 4-month growth condition, the tissue culture seedlings grow slowly, but the base parts also sprout.
Therefore, one or more steps in the steps of the invention are lacked, the ex vitro rooting of the tissue culture seedlings of the savatier monochasma cannot achieve the technical effect or result brought by the technical scheme of the invention on the survival rate and 4-month growth conditions, and further, the steps in the method of the steps of the invention need to be closely combined and cannot be separately and independently performed, otherwise, the technical effect of the invention cannot be achieved.
The above description is only an embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are included in the scope of the present invention.
Claims (9)
1. A method for rooting tissue culture seedlings of a medicinal plant, namely, the herb of pilose antler outside a bottle is characterized in that: the method comprises the following steps:
(1) selecting bottle seedlings: selecting hairy antler grass tissue culture seedlings with good growth vigor, long-woolly leaves and silvery white color to form cluster buds, and performing strong seedling culture;
(2) strong seedling culture: cutting off multiple buds of the tissue culture seedling in a single plant, selecting single buds with terminal buds and 2cm high of seedlings, transferring the single buds to a strong seedling culture medium, culturing for 20 days, and hardening the seedlings when the height of the seedlings is 3-5 cm;
(3) hardening seedlings: firstly, putting the velvet grass tissue culture seedlings in a seedling hardening room with a sunshade net, controlling the temperature at 25-28 ℃, culturing for a week, opening a bottle cap, and continuously culturing for 2-3 days.
(4) Matrix preparation and treatment: subpackaging the mixed and prepared transplanting matrix into seedling culture containers, thoroughly watering the seedling culture containers, and disinfecting the matrix by using potassium permanganate and carbendazim;
(5) rooting outside the tissue culture seedling bottle: taking out the seedlings from the bottle, washing the culture medium at the base part of the plants with tap water, soaking the seedlings in rooting water before cuttage, wherein the cuttage depth is preferably 0.5-1.0 cm, and covering a thin film arched shed;
(6) managing after planting: after 2 weeks of planting, new roots are seen at the base part, the film can be lifted off, the number of the roots reaches 5-10 after 30 days, the root length is 0.5-1.5 cm, and then leaf fertilizer and bactericide are regularly sprayed; robust velvet grass seedlings with the seedling height of about 6-10 cm can be obtained in about 2 months.
2. The method for rooting tissue culture seedlings of the medicinal plant, namely the pilose antler grass outside the bottle as claimed in claim 1, is characterized in that: the strong seedling culture medium in the step (2) contains the following components:
macroelements: KNO3:1200mg/L;NH4NO3:1650mg/L;Ca(NO3)2·4H2O:400mg/L; KH2PO4:170mg/L;MgSO4·7H2O:370mg/L;CaCl2·7H2O:240mg/L;
Organic components: inositol: 200 mg/L; glycine: 2 mg/L; thiamine hydrochloride: 0.4 mg/L; pyridoxine hydrochloride: 0.5 mg/L; nicotinic acid: 0.5 mg/L; the other components are the same as MS.
3. The method for rooting tissue culture seedlings of the medicinal plant, namely the cornua cervi pantotrichum in vitro according to claim 2, is characterized in that: the formula of the culture medium is as follows: improved MS + BA0.3mg/L + IAA 0.5-1.0 mg/L + GA 0.2-0.4 mg/L + sucrose 30g/L + agar 1.8-2.0 g/L.
4. The method for rooting tissue culture seedlings of the medicinal plant, namely the pilose antler grass outside the bottle as claimed in claim 1, is characterized in that: in the step (3), the illumination intensity in the seedling hardening room is enhanced to 3000Lux from 1800-2000 Lux in the culture room, and the humidity is controlled to be 60-80%.
5. The method for rooting tissue culture seedlings of the medicinal plant, namely the pilose antler grass outside the bottle as claimed in claim 1, is characterized in that: the formula of the substrate in the step (4) is 20-30% of peat, 20% of decomposed bark, 10-20% of carbonized chaff and 30% of yellow soil (by volume ratio), and all the components are fully and uniformly mixed according to the proportion.
6. The method for rooting tissue culture seedlings of the medicinal plant, namely the pilose antler grass outside the bottle as claimed in claim 1, is characterized in that: and (4) sprinkling a mixed solution of 0.5% of potassium permanganate and 0.08% of carbendazim solution by using a sprinkling can for matrix disinfection, and covering the disinfected film for more than 10 hours.
7. The method for rooting tissue culture seedlings of the medicinal plant, namely the pilose antler grass outside the bottle as claimed in claim 1, is characterized in that: preparing a rooting solution in the step (5): 500ppm-1000ppm ABT and 1000ppm 6-BA, the time for soaking the rooting agent is 30 s-60 s, and the base part of the tissue culture seedling is soaked for 0.5-1.0 cm.
8. The method for rooting tissue culture seedlings of the medicinal plant, namely the pilose antler grass outside the bottle as claimed in claim 1, is characterized in that: and (4) the foliar fertilizer in the step (6) is prepared by alternately using a large amount of urea and MS solutions and spraying the solutions once every 3 days.
9. The method for rooting tissue culture seedlings of the medicinal plant, namely the pilose antler grass outside the bottle as claimed in claim 1, is characterized in that: in the step (6), the bactericide comprises one or more of carbendazim, thiophanate methyl, Bordeaux mixture and chlorothalonil, and is mixed and used alternately, and the concentration is preferably 1000-1500 ppm.
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| CN111386981A (en) * | 2020-04-10 | 2020-07-10 | 惠州市九惠制药股份有限公司 | Method for planting savatier saururus in pot |
| CN111386981B (en) * | 2020-04-10 | 2022-03-18 | 惠州市九惠制药股份有限公司 | Method for planting savatier saururus in pot |
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| CN113692926B (en) * | 2020-11-04 | 2023-02-14 | 宜春市炳晨农业科技发展有限公司 | Method for growing seedlings of savatier monochasma herb by using non-woven fabric bags |
| CN113812337A (en) * | 2021-04-02 | 2021-12-21 | 宜春市炳晨农业科技发展有限公司 | Hydroponic rooting method for savatier monochasma |
| CN113678696A (en) * | 2021-04-07 | 2021-11-23 | 宜春市炳晨农业科技发展有限公司 | Seedling growing method for savatier monochasma herb |
| CN113678696B (en) * | 2021-04-07 | 2023-03-07 | 宜春市炳晨农业科技发展有限公司 | Seedling growing method for savatier monochasma herb |
| CN113207571A (en) * | 2021-06-08 | 2021-08-06 | 中国医学科学院药用植物研究所 | Parasitic cultivation method for savatier monochasma herb |
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