KR101390396B1 - Multiplication method of monocotyledon by removing stipes and by treating low temperature - Google Patents

Abstract

The present invention relates to a propagation method of monocotyledons, and more specifically, to a method which propagates monocotyledons such as rhodea, hosta longipes, kafir-lily, etc. in an economical method but minimizes changes in genetic traits, thereby maintaining leaf patterns of a mother plant and propagating the monocotyledons.

Description

[0001] The present invention relates to a method for growing a monocotyledonous plant by removing a petiole,

The present invention relates to a method of propagating a monocotyledonous plant, and more particularly, to a method of propagating monocotyledonous plants such as Manchuria, Vivicula, and Gunjaran by a more economical method, .

Plants breed by seed breeding, spore breeding, and nutrition breeding. Representative plant propagation methods include sowing, dispensing, cutting, bulb dividing, cutting, and grafting.

The seeding method is to sow directly in the field or in the field, and expensive seeds, rare seeds, plants with low germination rate, and seeds with very small seeds. Mass reproduction is easy.

The method of dividing refers to the method of planting the abandonment of abscissa or tall trees, and in the case of the plant of plants, propagation is made largely, and when the abandonment is weakened, it is carried out with the attendant to revive the vitality and to correct the shape.

It is a method of rooting and germination by applying a rooting accelerator to a part of the tree branch (a branch near the ground) and burrowing it in the soil. This is called horizontal fertilization. When the end of the branch is buried, there is an end-filling. In the root part is the embankment. In the middle of the branch, there is an annular peel method in which the surface of the branch is cut out. The success rate is very high.

The bulb division is a method of dividing naturally occurring roots such as gladiolus, tulip, crocus, etc. during cultivation. Hyacinth, and amaryllis are artificially promoted by self-assembly. Canna, Dahlia, etc. are cut and planted by attaching parts with eyes.

It is a propagation method that uses the regenerative power of the plant by cutting a part of the body of the plant. The plant is divided into two branches according to the cutting (branching material), branching-leaf (rubber tree, cactus, (Root of roots) (root of roots (roots of Japanese peony, Japanese primate, Japanese primrose, stoop posterior) are cut by about 10cm).

The grafting method is a breeding method using a plant having affinity as a reproduction method using the regenerative power of a plant as a cutting, and a kind of propagation method in which a kind such as acceptance is subjected to cutting (usually stems and gums) using a wood.

Monocotyledonous plants such as Viviculae, Mannyeongcheong, and Gunjaran usually observe the formation of snow before budding, that is, autumn or spring budding.

The axillary bud is the bud formed between the stem and stem of the plant. These eyes grow in some branches depending on the plant, and some remain in snow. However, when the child is absent, the child under the right child grows at a rapid rate to replace the child. This action is an apical dominance phenomenon, and this positive dominance phenomenon is destroyed by cytokinins treatment to develop the adventitia or to develop adventitious child uses a lot of cytokinin.

It was found that the cleft palate of the apical meristems disappeared even after kinetin, a kind of cytokinin, disappeared, and subsequently ABA was widely used to develop adventitia or adventitia in infant reproduction. In the plants in which the axes are formed, the number of new axons can be increased due to the large number of axes, and the axes and lobes grown by these axes are used again as breeders. Therefore, plants that are well propagated do not need to be cultivated separately. When an animal is separated by a slice, a large animal is used only for its large size, but a small animal is usually inoculated by including a part of a stem or a whole node, so that the animal may sometimes be cultured.

Hosta longipes is a perennial plant belonging to the Liliaceae, and all the leaves come out of the roots and become rosettes, and flower stalks come out between the leaves. Leaves are 10 ~ 15㎝ long, 7 ~ 9㎝ wide, and the edges of the leaves are slightly wrinkled. There are wings on the petiole, and it gradually fades toward the root. In summer, purple flowers are alternated with each other on the stem, and the end of the flower is divided into 6 branches. The fruit is ripe in a long oval shape. It grows frequently in the shady mountain stream. Young leaves may also be eaten as herbs.

Rohdea japonica is an evergreen perennial plant belonging to the Lily family (Liliaceae). It is originated in Japan and is widely planted as a houseplant in Korea. In the coarse ground stem, 9-12 leaves gather together, but the stem does not grow big. The leaves are 30 ~ 50㎝ long, 3 ~ 5㎝ wide, and are thicker, pointed with a sharp tip, and the tip of the leaf hangs down when planted in a flowerpot. Leaf edges are wrinkled like waves. The flower is yellow or light green and has a cup shape, and it tends to swell over the flower stalk that emerges from the leaf between May and July. The corolla is divided into 6 branches and 6 stamens are attached to corolla. There are 1 pistil and 3 stigmas. The fruit is berries, and it ripens in red in autumn. Its appearance is similar to that of corn. Be sure to take it in the winter when it is winter, grow well in the half sidewalk, and be careful in the summer when the sun is on the spot, because the leaves are dry and dry. Some varieties have white stripes on the edge of the leaves or yellowish-white stripes on the green leaves. In Japan, it has been planted since ancient times, but it also uses rootstock as a cardiopulmonary or diuretic. R. discolor is widely planted in the greenhouse or house originating in Mexico and the West Indies, and it feeds on diarrhea caused by plant poisoning, and indigestion of young children.

Clivia miniata is an evergreen perennial plant belonging to the family Amaryllidaceae. It is a horticultural plant originating in southern Africa, and is often planted as a corn plant in a greenhouse or house in Korea. Leaves, like the stem of the scales, wrap around each other and come out on both sides. They are 45㎝ long and 5㎝ wide, and are shiny and the leaf tips are bent backward. A peduncle comes out among the leaves, forming an umbrella flower, with about 10 to 20 birds in a funnel shape. Fruits ripen in light red color. It is better to divide the young shoots from individuals who have been growing for six to seven years during April. It also grows with seeds, but after 3 to 4 years after planting the seeds, the flowers bloom. It grows well in watery, shady, windy areas, in fields, and in the soil of rice fields.

In recent years, attempts have been made to preserve native plant resources and to industrialize them. In the case of foreign countries, there are many cultivars breeding with native plants. However, in Korea, research and development of native plant resources is insufficient. In addition, it is economically advantageous to select and fix domestic native plant species to import and breed new plant varieties. For this purpose, a mass production system of domestic native plant species should be established and specialized.

Japanese Patent No. 7,152,848 discloses a method for culturing stem cells, Treating the induced stem with cytokinin to proliferate the stem; Treating the proliferated stem with dioxin to induce roots; And planting the seedlings by purifying the regenerated seedlings and transplanting the seedlings into a pod, thereby providing a method for producing seedlings of ginseng seedlings by subculturing.

Open Patent No. 1988-11326 discloses a method of producing and storing pure tuberous tubers in vitro in the production of high quality potatoes (Solanum tuverosum L.) and using them in a Murashige & Skoog medium The present invention provides a method for mass production of a test and a tuber in a short period of time at a stem portion of a stem by inoculating a large-scale proliferated potato sprout into a test tube medium set in a culture room under a predetermined condition.

However, the above methods are a method for mass production of seedlings or tubers through culture, and it is necessary to treat medicines such as cytokinin and ooxine, and expensive equipments such as clean rooms and culture facilities are needed.

In addition, monocotyledonous plants such as Viviculae, Mannyeongcheong, and Gunjaran are preferred to non-irregular species with no pattern on the leaves. However, when it is propagated by a tissue culture method or a seed propagation, there is a problem that most of the plants are unstable due to a large variation, resulting in untreated paper.

Accordingly, it is an object of the present invention to provide a method of propagating a plant by a more economical and simple method in the propagation method of monocotyledonous plants such as Vivicula, Mannyeongcheong and Gunjoran.

It is another object of the present invention to provide a plant propagation method of maintaining a leaf pattern of a previous generation in a method of propagating a monocotyledonous plant such as a grapevine, a perennial plant, a grapevine, and the like.

In order to achieve the above object, the inventor of the present invention has found that, during the summer, the petiole of camphor plants such as Vivienne, Manchuria and Gunjoran are removed from the outside in sequence, And then proliferating at room temperature.

The present invention

a) removing the petiole from the outer petiole in turn, further removing the petiole when the petiole is observed in the inner petiole;

b) disrupting the dormant plants by treating the monocotyledonous plants from which the petiole has been removed at a low temperature of 2-6 캜; And

c) culturing the monocotyledonous plants treated at the low temperature in an appropriate growing temperature, and removing the petiole and treating the monocotyledonous plants by low temperature treatment.

At this time, if the low-temperature treatment temperature is lower than 2 ° C or higher than 6 ° C, proliferation of the liquid is inhibited.

Sequential removal of petiole promotes the development of the embryo.

The term "optimum temperature for growth" in the above c) means a temperature suitable for the growth of each plant. The optimal temperature for growth of the nonvowel is 15 to 30 캜, and the optimum temperature for growth of the perennial plant is 20 to 25 캜. Is well known to those of ordinary skill in the art.

The present invention provides a method for growing monocotyledonous plants by petiole treatment and low temperature treatment, wherein the low temperature treatment in step b) is carried out for 10 to 60 days.

At this time, if the treatment at a low temperature is shorter than 10 days, sufficient proliferation of the embryo is inhibited, and even if it exceeds 60 days, the proliferation of the embryo is lowered due to long term low temperature and dark state.

In addition, the present invention provides a method for growing monocotyledonous plants by removing petiole and treating at low temperature, wherein the monocotyledonous plants are non-viviparous, perennial or gonorrhoeae.

According to the propagation method of the present invention, it is possible to obtain a monocotyledonous plant which inherits the leaves of the mother liquor.

In addition, according to the propagation method of the present invention, it is possible to increase the number of monocotyledonous plants by a simple method such as removal of petiole and low temperature treatment, thereby saving labor and economical efficiency.

FIG. 1 is a photograph taken during the removal of a petiole of a perennial plant.
FIG. 2 is a photograph of an elevation after removal of a part of a lunar New Year bottle.
FIG. 3 is a photograph showing low-temperature treatment after removal of pistil leaves. From the left, the photographs are 10 days, 20 days, 40 days, and 60 days after cold treatment, respectively.
Fig. 4 is a photograph taken during the removal of a petiole of a vivienus.
FIG. 5 is a photograph of an elevation after removing all the petiole of the vivienne.
FIG. 6 is a photograph of a viviparous part in which an embryo developed by low-temperature treatment after removal of a non-avirulent petiole.
FIG. 7 is a photograph showing the growth of a rabbit after 10 days of low temperature treatment after removing a petiole.
FIG. 8 is a photograph showing the growth of a rabbit after 20 days of low-temperature treatment after removing a petiole.
FIG. 9 is a photograph showing the growth of a rabbit after 40 days of low temperature treatment after removing a petiole.
FIG. 10 is a photograph showing the growth of a rabbit after 60 days of low temperature treatment after removing a petiole.

Hereinafter, the configuration of the present invention will be described in detail with reference to specific embodiments. However, it is apparent to those skilled in the art that the scope of the present invention is not limited by the descriptions of the embodiments.

≪ Example 1:

The longevity and lunar year lakes were planted in the port. Each of the 100 ports was untreated, and each of the 100 ports was subjected to a leaf cutting process. In addition, 100 pots were removed from the outer petiole in order to remove petiole, and then placed in a low temperature reservoir at 5 ° C for dormancy breaking.

The number of newborn babies occurring at this time is shown in Table 1 below.

division Number of ports Processing method Shin Aso
Perennial (lakeside)
No treatment 100 - 28 Floss treatment 100 Remove the ground part in the summer 26 Laceration treatment 100 Sequential removal of petiole 59
Year-old (mountain)
No treatment 100 - 40 Floss treatment 100 Remove the ground part in the summer 23 Laceration treatment 100 Sequential removal of petiole 68

≪ Example 2: Perennial low temperature treatment >

When lakes are formed, the petiole is removed in order, leaving a part of the petiole in the middle part. In the low temperature reservoir at 5 ℃, , 40 days, and 60 days, respectively.

The results are shown in Table 2 below.

Perennial (lakeside)

Treatment temperature 5 ℃ 5 ℃ 5 ℃ 5 ℃ Refrigerated days 10 days 20 days 40 days 60 days Treatment (port) 100 100 100 100 Number of newborn infants 59 57 63 61
Year-old (mountain)

Treatment temperature 5 ℃ 5 ℃ 5 ℃ 5 ℃ Refrigerated days 10 days 20 days 40 days 60 days Treatment 100 100 100 100 Number of newborn infants 66 69 71 65

≪ Example 3:

The middle part of the rabbit and the rabbits were cultivated in the pot. Each of the 100 ports was untreated, and each of the 100 ports was subjected to a leaf cutting process. In addition, 100 pots were removed from the outer petiole in order to remove petiole, and then placed in a low temperature reservoir at 5 ° C for dormancy breaking.

The number of newborn babies occurring at this time is shown in Table 3 below.

division Number of ports Processing method Shin Aso
Vivace (mid)
No treatment 100 - 30 Floss treatment 100 Remove the ground part in the summer 48 Laceration treatment 100 Sequential removal of petiole 78
A rabbit
No treatment 100 - 35 Floss treatment 100 Remove the ground part in the summer 59 Laceration treatment 100 Sequential removal of petiole 81

≪ Example 4: Vivo low temperature treatment >

In the case of an embryo, the petiole was removed in order to leave a part of the petiole in the center, or the petiole was completely removed, and the petiole was removed in the low temperature reservoir at 5 ° C 10 days, 20 days, 40 days, and 60 days.

The results are shown in Table 4 below.

Vivace (mid)

Treatment temperature 5 ℃ 5 ℃ 5 ℃ 5 ℃ Refrigerated days 10 days 20 days 40 days 60 days Treatment (port) 100 100 100 100 Number of newborn infants 72 70 101 53
A rabbit

Treatment temperature 5 ℃ 5 ℃ 5 ℃ 5 ℃ Refrigerated days 10 days 20 days 40 days 60 days Treatment 100 100 100 100 Number of newborn infants 48 52 83 60

Claims (3)

a) removing one or more petiole from the outer petiole in order to remove one petiole when the petiole is observed inside the petiole;
b) disrupting the dormant plants by treating the monocotyledonous plants from which the petiole has been removed at a low temperature of 2-6 캜; And
c) propagating the low temperature treated monocotyledonous plant at an appropriate growth temperature, and removing the petiole and treating the monocotyledonous plant by low temperature treatment.
The method according to claim 1,
Wherein the low temperature treatment in step b) is carried out for 10 to 60 days.
delete

Images