CN104255480A - Rapid propagation method of cyclobalanopsis glauca - Google Patents
Rapid propagation method of cyclobalanopsis glauca Download PDFInfo
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- CN104255480A CN104255480A CN201410463247.7A CN201410463247A CN104255480A CN 104255480 A CN104255480 A CN 104255480A CN 201410463247 A CN201410463247 A CN 201410463247A CN 104255480 A CN104255480 A CN 104255480A
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Abstract
The invention provides a rapid propagation method of cyclobalanopsis glauca. The rapid propagation method comprises the steps of sterilization of a stem, induction of cluster buds, multiplication of cluster buds, rooting culture, acclimatization and transplanting, and the like. The cyclobalanopsis glauca prepared by the rapid propagation method is high in survival rate, good in quality, short in growth cycle and good in genetic stability, and can be popularized and planted in a large scale.
Description
Technical field
The present invention relates to the quick-breeding method of Qinggang tissue cultures, belong to plant technology field.
Background technology
Qing Gangshu,
cyclobalanopsis glauca (Thunb.) Oerst, Fagaceae, have another name called Oak Tree, acorn nut tree, Qinggang oak, aiphyllium is tropical trees, up to 20 meters.Leaf alternate, Ji Zhiding, keratin, oval, the shape of falling ovum is oval, and the short acumen of leaf top tool, the wide wedge shape of phyllopodium is circular, the above tool tooth of leaf central part.The several raw branch top armpit of female flower.Acorn-cup bowl-type, footpath 1 cm; Nut elliposoidal, long 1.2-1.7 centimetre, 1 centimetre, footpath.One of the widest seeds of China's distribution.Also there are distribution in Korea, Japan, India.Northern subtropical deciduous-evergreen broadleaf mixed forest district of the Changjiang river areas to the south, responsive to weather conditions reaction, neutral happiness light, children's age slightly resistance to lateral shelter.Happiness is born on the limestone rocky mountain of alkalescence or neutrality, also well-grown on acid ground.The deep-rootedness system of taproot, resistance to drying, can be grown on the mountain region of many chads.Rudiment power is strong, can adopt Sprout reproduction.Bark, leaf: bitter, puckery, tepor bark, leaf: convergence, only dysentery.For rushing down dysentery for a long time.Really: removing toxicity for detumescence.For mastitis, do not have the research of cultivating and growing at present, tissue culture method survival rate is high, and production cost is low, and growth cycle is short, is beneficial to large-scale promotion plantation.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of method for quickly breeding of Qinggang, high by the method preparation-obtained Qinggang survival rate, quality better, and growth cycle is short, and genetic stability is good, is beneficial to large-scale promotion plantation.
Technical problem to be solved by this invention is realized by following scheme:
Qinggang is newly taken out slightly, be cut into about 2cm, with the segment of axillary-bud or top-bud, first in bleaching powder, soak 3min, surface blot removed by hairbrush, running water 45min, 400 μ g/g benzyl ammonia blue or green liquid process 20min on superclean bench, aseptic water washing 5-7 time, Qinggang stem with bud access culture medium prescription that sterilization treatment is crossed is N
6carry out axillary bud deriving cultivation in+6-BA1.0mg/L+NAA0.1mg/L inducing culture, additional sugar 30g/L, agar 6.5g/L, pH5.8, illumination 2500lx, temperature 26 ± 1 DEG C, the bud derived puts into medium 1/2N
6+ IAA0.2mg/L+50mg/L tomato juice carries out Multiplying culture, and additional sugar 30g/L, agar 6.5g/L, pH5.8, illumination 3000lx, temperature 26 ± 1 DEG C, Multiplying culture Multiple Buds out puts into root media 3/4N
6+ NAA1.0mg/L+90mg/L cysteine+sucrose 20g/L, agar 6.5g/L, pH5.8, illumination 4000lx, temperature 28 DEG C, test-tube plantlet is about 5cm, takes out from blake bottle, at 0.5mg/LABT
1soak 10 minutes in root-inducing powder, be colonizated in the red heart soil that high-temperature sterilization is crossed: in chaff ash=3:1, density is 18 × 16cm, shelters from heat or light 40%, illumination 5000lx, temperature 27 DEG C, humidity 60%, regularly water, bacterium ester 2000-3000 times of liquid is killed in sprinkling in 15 days for one time 20%, adds up survival rate after 30 days.
Qinggang survival rate adopting the present invention to prepare is 90%, and the cycle is short, and output is large, pollutes little, is beneficial to implant mass.
Below in conjunction with embodiment, the present invention is further elaborated, but the scope of protection of present invention is not limited to following embodiments.
Embodiment
Embodiment 1
Qinggang is newly taken out slightly, be cut into about 2cm, with the segment of axillary-bud or top-bud, first in bleaching powder, soak 3min, surface blot removed by hairbrush, running water 45min, 400 μ g/g benzyl ammonia blue or green liquid process 20min on superclean bench, aseptic water washing 5-7 time, Qinggang stem with bud access culture medium prescription that sterilization treatment is crossed is N
6carry out axillary bud deriving cultivation in+6-BA1.0mg/L+NAA0.1mg/L inducing culture, additional sugar 30g/L, agar 6.5g/L, pH5.8, illumination 2500lx, temperature 26 ± 1 DEG C, the bud derived puts into medium 1/2N
6+ IAA0.1mg/L+50mg/L tomato juice carries out Multiplying culture, and additional sugar 30g/L, agar 6.5g/L, pH5.8, illumination 3000lx, temperature 26 ± 1 DEG C, Multiplying culture Multiple Buds out puts into root media 3/4N
6+ NAA0.05mg/L+80mg/L cysteine+sucrose 20g/L, agar 6.5g/L, pH5.8, illumination 4000lx, temperature 28 DEG C, test-tube plantlet is about 5cm, takes out from blake bottle, at 0.5mg/LABT
1soak 10 minutes in root-inducing powder, be colonizated in the red heart soil that high-temperature sterilization is crossed: in chaff ash=3:1, density is 18 × 16cm, shelters from heat or light 40%, illumination 5000lx, temperature 27 DEG C, humidity 60%, regularly water, bacterium ester 2000-3000 times of liquid is killed in sprinkling in 15 days for one time 20%, within 30 days, adds up survival rate 85% afterwards.
Embodiment 2
Qinggang is newly taken out slightly, be cut into about 2cm, with the segment of axillary-bud or top-bud, first in bleaching powder, soak 3min, surface blot removed by hairbrush, running water 45min, 400 μ g/g benzyl ammonia blue or green liquid process 20min on superclean bench, aseptic water washing 5-7 time, Qinggang stem with bud access culture medium prescription that sterilization treatment is crossed is N
6carry out axillary bud deriving cultivation in+6-BA1.0mg/L+NAA0.1mg/L inducing culture, additional sugar 30g/L, agar 6.5g/L, pH5.8, illumination 2500lx, temperature 26 ± 1 DEG C, the bud derived puts into medium 1/2N
6+ IAA0.3mg/L+50mg/L tomato juice carries out Multiplying culture, and additional sugar 30g/L, agar 6.5g/L, pH5.8, illumination 3000lx, temperature 26 ± 1 DEG C, Multiplying culture Multiple Buds out puts into root media 3/4N
6+ NAA1.0mg/L+100mg/L cysteine+sucrose 20g/L, agar 6.5g/L, pH5.8, illumination 4000lx, temperature 28 DEG C, test-tube plantlet is about 5cm, takes out from blake bottle, at 0.5mg/LABT
1soak 10 minutes in root-inducing powder, be colonizated in the red heart soil that high-temperature sterilization is crossed: in chaff ash=3:1, density is 18 × 16cm, shelters from heat or light 40%, illumination 5000lx, temperature 27 DEG C, humidity 60%, regularly water, bacterium ester 2000-3000 times of liquid is killed in sprinkling in 15 days for one time 20%, within 30 days, adds up survival rate 87% afterwards.
Embodiment 3
Qinggang is newly taken out slightly, be cut into about 2cm, with the segment of axillary-bud or top-bud, first in bleaching powder, soak 3min, surface blot removed by hairbrush, running water 45min, 400 μ g/g benzyl ammonia blue or green liquid process 20min on superclean bench, aseptic water washing 5-7 time, Qinggang stem with bud access culture medium prescription that sterilization treatment is crossed is N
6carry out axillary bud deriving cultivation in+6-BA1.0mg/L+NAA0.1mg/L inducing culture, additional sugar 30g/L, agar 6.5g/L, pH5.8, illumination 2500lx, temperature 26 ± 1 DEG C, the bud derived puts into medium 1/2N
6+ IAA0.3mg/L+50mg/L tomato juice carries out Multiplying culture, and additional sugar 30g/L, agar 6.5g/L, pH5.8, illumination 3000lx, temperature 26 ± 1 DEG C, Multiplying culture Multiple Buds out puts into root media 3/4N
6+ NAA0.05mg/L+90mg/L cysteine+sucrose 20g/L, agar 6.5g/L, pH5.8, illumination 4000lx, temperature 28 DEG C, test-tube plantlet is about 5cm, takes out from blake bottle, at 0.5mg/LABT
1soak 10 minutes in root-inducing powder, be colonizated in the red heart soil that high-temperature sterilization is crossed: in chaff ash=3:1, density is 18 × 16cm, shelters from heat or light 40%, illumination 5000lx, temperature 27 DEG C, humidity 60%, regularly water, bacterium ester 2000-3000 times of liquid is killed in sprinkling in 15 days for one time 20%, within 30 days, adds up survival rate 88% afterwards.
Claims (4)
1. the method for quickly breeding in Qinggang, comprise the sterilization of stem section, the induction of Multiple Buds, the propagation of Multiple Buds, culture of rootage, acclimatization and transplants, its key step is as follows:
(1) get Qinggang newly to take out slightly, be cut into about 2cm, the segment of band axillary-bud or top-bud, to its disinfection;
(2) getting Qinggang stem with bud access culture medium prescription that step (1) sterilization treatment crosses is N
6axillary bud deriving cultivation is carried out, additional sugar 30g/L, agar 6.5g/L, pH5.8, illumination 2500lx, temperature 26 ± 1 DEG C in+6-BA1.0mg/L+NAA0.1mg/L inducing culture;
(3) get the bud that step (2) derives and put into medium 1/2N
6+ IAA0.1-0.3mg/L+50mg/L tomato juice carries out Multiplying culture, additional sugar 30g/L, agar 6.5g/L, pH5.8, illumination 3000lx, temperature 26 ± 1 DEG C;
(4) get step (3) Multiplying culture Multiple Buds out and put into root media 3/4N
6+ NAA0.05-1.0mg/L+80-100mg/L cysteine+sucrose 20g/L, agar 6.5g/L, pH5.8, illumination 4000lx, temperature 28 DEG C;
(5) Qinggang test-tube plantlet after taking root carries out field acclimatization and transplants.
2. according to the method for quickly breeding in a kind of Qinggang according to claim 1, it is characterized in that: the acquisition of Qinggang aseptic explant described in step (1) is, get Qinggang stem with bud, first in bleaching powder, soak 3min, surface blot removed by hairbrush, running water 45min, 400 μ g/g benzyl ammonia blue or green liquid process 20min, aseptic water washing 5-7 time on superclean bench.
3. according to the method for quickly breeding in a kind of Qinggang according to claim 1, it is characterized in that: in step (3), adventitious buds proliferation adds tomato juice in cultivating, and can promote that the adventitious buds proliferation in Qinggang is cultivated.
4. according to the method for quickly breeding in a kind of Qinggang according to claim 1, it is characterized in that: the acclimatization and transplants method in step (5) is that test-tube plantlet is about 5cm, takes out, at 0.5mg/LABT from blake bottle
1soak 10 minutes in root-inducing powder, be colonizated in the red heart soil that high-temperature sterilization is crossed: in chaff ash=3:1, density is 18 × 16cm, shelters from heat or light 40%, illumination 5000lx, temperature 27 DEG C, humidity 60%, regularly water, bacterium ester 2000-3000 times of liquid is killed in sprinkling in 15 days for one time 20%, adds up survival rate after 30 days.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410463247.7A CN104255480A (en) | 2014-09-12 | 2014-09-12 | Rapid propagation method of cyclobalanopsis glauca |
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| CN201410463247.7A CN104255480A (en) | 2014-09-12 | 2014-09-12 | Rapid propagation method of cyclobalanopsis glauca |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106718939A (en) * | 2017-02-15 | 2017-05-31 | 唐春艳 | A kind of red skin Qinggang spray tissue culture propagation method |
| CN106718937A (en) * | 2017-02-14 | 2017-05-31 | 唐春艳 | The method that Qinggang tree obtains aseptic explant preparation and initial bud |
| CN106797888A (en) * | 2017-02-15 | 2017-06-06 | 唐春艳 | The rapid propagation method of Qinggang tree tissue cultures |
| CN106888966A (en) * | 2017-02-14 | 2017-06-27 | 唐春艳 | Red skin Qinggang aseptic explant is prepared and initial bud abductive approach |
-
2014
- 2014-09-12 CN CN201410463247.7A patent/CN104255480A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106718937A (en) * | 2017-02-14 | 2017-05-31 | 唐春艳 | The method that Qinggang tree obtains aseptic explant preparation and initial bud |
| CN106888966A (en) * | 2017-02-14 | 2017-06-27 | 唐春艳 | Red skin Qinggang aseptic explant is prepared and initial bud abductive approach |
| CN106718939A (en) * | 2017-02-15 | 2017-05-31 | 唐春艳 | A kind of red skin Qinggang spray tissue culture propagation method |
| CN106797888A (en) * | 2017-02-15 | 2017-06-06 | 唐春艳 | The rapid propagation method of Qinggang tree tissue cultures |
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Application publication date: 20150107 |