CN106718939A - A kind of red skin Qinggang spray tissue culture propagation method - Google Patents
A kind of red skin Qinggang spray tissue culture propagation method Download PDFInfo
- Publication number
- CN106718939A CN106718939A CN201710079784.5A CN201710079784A CN106718939A CN 106718939 A CN106718939 A CN 106718939A CN 201710079784 A CN201710079784 A CN 201710079784A CN 106718939 A CN106718939 A CN 106718939A
- Authority
- CN
- China
- Prior art keywords
- culture
- bud
- explant
- red skin
- strong
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a kind of red skin Qinggang spray tissue culture propagation method, including explant selection, explant treatment, initial bud inducement cultivation, Multiplying culture, strong bud culture and culture of rootage operation, collection semi-lignified, red skin Qinggang spray raw then of robust growth is used as explant, explant is pruned, sterilization obtains initial bud during initial bud inducement cultivation base is inoculated in after processing, initial bud is inoculated in proliferated culture medium by 34 cycle proliferation cultures again, form Multiple Buds, cultivated during Multiple Buds are accessed into strong buds medium, finally by root induction in the simple bud insertion root media of height >=2cm.Present invention reduces the growing-seedling period in red skin Qinggang, nursery material is saved, the problems such as nursery material requested is more, the cycle is long present in traditional seedling raising method are overcome, production cost is greatly reduced, production efficiency is improve, with preferable economic benefit, social benefit and ecological benefits.
Description
Technical field
The invention belongs to technical field of plant propagation, it is related to a kind of red skin Qinggang spray tissue culture propagation method.
Background technology
Red skin Qinggang (Cyclobalanopsis gilva (Blume) Oerst), belongs to Fagaceae Cyclobalanopsis
(Cyclobalanopsis Oerst) plant, is aiphyllium, and up to 30 meters, bark crineous, impoverishment tolerant is extensive in China
It is distributed in the province such as Zhejiang, Hunan.Red skin Qinggang heartwood is in dark reddish brown, and sapwood is in yellowish-brown, and texture is straight, and the hard weight of matter is tough to have
Elasticity, 0.85-0.91 grams/cc of air-dry density, widely used, economic worth is very high, is the precious hard wood species of China
One of.Due to a large amount of felling, endangered species have been become.On the one hand, red skin Qinggang seed belongs to Recalcitrant Seeds, its sprout time
Very long, germination percentage is relatively low, and easily suffers insect pest, and not easy to maintain, survival rate is very low, and generative propagation speed is slow；On the other hand, it is sexual
Breeding it cannot be guaranteed that the good characteristic of forest, however, tissue cultures can be by vegetative propagation, the good characteristic of hereditary seeds, energy
Quick breeding in a short time obtains the tissue-cultured seedling of a large amount of high-quality, is promoted for high-quality introduces a collection and provides possibility.
The content of the invention
Bud growth coefficient is low during the purpose of the present invention is directed to red skin Qinggang tissue culture procedures, the problems such as slow-growing,
There is provided that a kind of growth result is good, expand numerous fast red skin Qinggang spray tissue culture propagation method.
In order to realize foregoing invention purpose, technical scheme is as follows：
A kind of red skin Qinggang spray tissue culture propagation method, including explant selection, explant treatment, initial bud inducement cultivation, increasing
Culture, strong bud culture and culture of rootage operation are grown, collection semi-lignified, red skin Qinggang spray raw then of robust growth are used as outer
Implant, is pruned to explant, is inoculated in initial bud inducement cultivation base after sterilization treatment and obtains initial bud, then will be just
Multiple Buds are accessed strong buds medium by beginning bud in being inoculated in proliferated culture medium by 3-4 cycle proliferation culture, formation Multiple Buds
Middle culture, finally by root induction in the simple bud insertion root media of height >=2cm, concrete operation step is as follows：
（1）Explant is selected：Collection semi-lignified, red skin Qinggang spray raw then of robust growth are used as explant；
（2）Explant treatment：Explant is placed under running water and rinses 10-15 min, then remove explant blade, then will be outer
Implant is immersed in the mercuric chloride solution of volumetric concentration 0.1%, controls soak time 15-20 min, finally cleans medicine with pure water
Thing, band at least one axillary bud is cut into by explant, stem section 1.5-3cm long, depending in stem section degree of lignification classified placing container,
It is standby；
（3）Initial bud inducement cultivation：By step（2）The explant for obtaining is cultivated in being inoculated in initial bud inducement cultivation base, culture
30-35 d, initial bud is sprouted, growth；
（4）Multiplying culture：Multiplying culture, 35-40 d switchings one are carried out during the initial bud of height >=1cm is accessed into proliferated culture medium
It is secondary, move in circles, through the 3-4 culture in cycle, form Multiple Buds；
（5）Strong bud culture：By step（4）The Multiple Buds for obtaining are cut, 4-5 bud/clump, in insertion strong buds medium, training
35-40 d are supported, strong bud is formed；
（6）Culture of rootage：By step（5）Root induction in the simple bud insertion root media of the height >=2cm for obtaining；Remaining budlet
Cong Zaici is inoculated in step（4）Proliferated culture medium in continue breed, then repeat step（5）, move in circles, obtain a large amount of
Strong bud.
The formula of above-described initial bud inducement cultivation base is：1/2 improvement WPM culture medium+6-BA 4.0-6.0 mg/L
The mg/L+ folic acid 10mg/L+VB of+NAA 2.0-3.0 mg/L+KT 1.0-2.0 mg/L+ZT 1.5-2.0 mg/L+ VC 152
The g/L of 15 mg/+ agar, 3.0 g/L+ sucrose 25.
The formula of above-described proliferated culture medium is：Improvement WPM culture medium+6-BA 6.0-8.0 mg/L+NAA 2.0-
3.0 mg/L+ZT 0.5-1.0mg/L+VC 15 mg/L+VB2 The g/L+ of 15 mg+L- Cys2s, 0 mg/L+ agar 3.6
The g/L of sucrose 30.
The formula of above-described strong buds medium is：Improvement WPM culture medium+6-BA 3.0-4.0 mg/L+NAA 1.0-
1.5 mg/L+VC 15 mg/L+VB2 The g/L of 15 mg+L- Cys2s, 0 mg/L+ agar, 3.5 g/L+ sucrose 25.
The formula of above-described root media is：1/2 improvement WPM culture medium+6-BA 1.5-2.5 mg/L+NAA
The g/L of 3.5 g/L+ sucrose of 1.0-1.5 mg/L+ agar 30.
The above component and envelope-bulk to weight ratio for improveing WPM culture mediums are：NH4NO3370mg/L, CaCl2·2H2O
82 mg/L, MgSO4·7H20 270 mg/L, KH2PO4170 mg/L, Ca (NO3)2·4H2O 180 mg/L, KI 0.83
Mg/L, MnSO4·H2O 22.4 mg/L, ZnSO4·7H2O 8.6 mg/L, Na2MoO4·2H2O 0.25 mg/L, CuSO4·
5H2O 0.025 mg/L, Na2EDTA 37.4 mg/L, FeSO4·7H2The mg/L of O 27.8, the mg/L of inositol 200, nicotinic acid 0.5
Mg/L, the mg/L of puridoxine hydrochloride 0.5, the mg/L of thiamine hydrochloride 1.0, the mg/L of glycine 2.0.
Above step（3）The cultivation control condition of described initial bud inducement cultivation is：Optical culture, illumination 500-1000
Lx, 20 ± 1 DEG C of cultivation temperature；Step（4）Described Multiplying culture, step（5）Described strong bud culture, step（6）Described
The cultivation control condition of culture of rootage is：Optical culture, illumination 2500-4000 lx, daily illumination 16 h, cultivation temperature 25-28
℃。
Relative to prior art, the present invention has the advantage that and has the beneficial effect that：
1st, the present invention gives birth to spray using red skin Qinggang as explant then, and by initial bud medium culture, initial bud is sprouted rate
More than 90%；By 3-4 cycle proliferation culture, Multiple Buds, bud growth coefficient 5/30d-7/30d are formed, then Multiple Buds are accessed
Cultivated in strong buds medium, finally by root induction in the simple bud insertion root media of height >=2cm, so as to shorten Chi Piqing
The growing-seedling period on ridge, saves nursery material, overcomes present in traditional seedling raising method that nursery material requested is more, the cycle is long etc. and asks
Topic, greatly reduces production cost, improves production efficiency.
2nd, by root induction in the simple bud insertion root media of height >=2cm, remaining budlet clump continues to breed training the present invention
Support so that material can multiple subculture, realize largely breeding, large-scale production is strengthened bud, realizes that the expansion of safrole type camphor tree is numerous.
3rd, the present invention is improved to WPM minimal mediums, while emphasis have adjusted being sprouted strong bud phase with red skin Qinggang
Close element so that culture medium is more scientific, more targetedly, be more easy to promote generation, propagation and the strong bud of the bud induction of red skin Qinggang, effect
Fruit is significantly.
4th, operating process of the present invention is easy, and cultivation cycle is short, and subculture bud yield is big, and Functionality, quality and appealing design, and growth coefficient reaches 5
Tissue-cultured seedling industrialization technology requirement above, with good economic benefit, ecological benefits and social benefit.
Specific embodiment
With reference to specific embodiment, the present invention will be further described.
Embodiment 1：
（1）Explant is selected：Collection semi-lignified, red skin Qinggang spray raw then of robust growth are used as explant；
（2）Explant treatment：Explant is placed in 10 min are rinsed under running water, then remove explant blade, then by explant
Body is immersed in the mercuric chloride solution of volumetric concentration 0.1%, controls the min of soak time 15, finally cleans medicine with pure water, will
Explant is cut into band at least one axillary bud, and stem section 1.5-3cm long is standby depending in stem section degree of lignification classified placing container；
（3）Initial bud inducement cultivation：By step（2）The explant for obtaining is cultivated in being inoculated in initial bud inducement cultivation base, is placed in
Optical culture, the lx of illumination 500, cultivate 30-35 d in the environment that 20 ± 1 DEG C of cultivation temperature, initial bud is sprouted, growth；Described is first
The beginning formula of bud inducement cultivation base is：The mg/ of 1/2 2.0 mg/L+KT of improvement WPM culture mediums 4.0 mg/L+NAA of+6-BA 1.0
The mg/L+ folic acid 10mg/L+VB of 1.5 mg/L+ VC of L+ZT 152 The g/L of 15 mg/+ agar, 3.0 g/L+ sucrose 25；
（4）Multiplying culture：Multiplying culture is carried out during the initial bud of height >=1cm is accessed into proliferated culture medium, optical culture, illumination is placed in
2500 lx, the h of daily illumination 16, in the environment of 25-28 DEG C of cultivation temperature, 35-40 d transfer once, move in circles, through 3-4
The culture in cycle, forms Multiple Buds；The formula of described proliferated culture medium is：The mg/L+ of improvement WPM culture mediums+6-BA 6.0
NAA 2.0 mg/L+ZT 0.5 mg/L+VC 15 mg/L+VB2 The g/L+ of 15 mg+L- Cys2s, 0 mg/L+ agar 3.6
The g/L of sucrose 30；
（5）Strong bud culture：By step（4）The Multiple Buds for obtaining are cut, 4-5 bud/clump, in insertion strong buds medium, are put
In optical culture, the lx of illumination 2500, the h of daily illumination 16 cultivate 35-40 d in the environment of 25-28 DEG C of cultivation temperature, form strong
Bud；The formula of described strong buds medium is：The mg/L+VC 15 of 3.0 mg/L+NAA of improvement WPM culture mediums+6-BA 1.0
mg/L+VB2 The g/L of 15 mg+L- Cys2s, 0 mg/L+ agar, 3.5 g/L+ sucrose 25；
（6）Culture of rootage：By step（5）In the simple bud insertion root media of the height >=2cm for obtaining, remaining budlet clump connects again
Plant in step（4）Proliferated culture medium in continue breed, then repeat step（5）, move in circles, obtain largely strong bud；It is raw
Root culture is placed in optical culture, the lx of illumination 2500, the h of daily illumination 16, root induction in the environment of 25-28 DEG C of cultivation temperature,
Rooting rate is 94%；The formula of described root media is：The mg/L+NAA 1.0 of 1/2 improvement WPM culture mediums+6-BA 1.5
The g/L of 3.5 g/L+ sucrose of mg/L+ agar 30.
The above component and envelope-bulk to weight ratio for improveing WPM culture mediums are：NH4NO3370mg/L, CaCl2·2H2O
82 mg/L, MgSO4·7H20 270 mg/L, KH2PO4170 mg/L, Ca (NO3)2·4H2O 180 mg/L, KI 0.83
Mg/L, MnSO4·H2O 22.4 mg/L, ZnSO4·7H2O 8.6 mg/L, Na2MoO4·2H2O 0.25 mg/L, CuSO4·
5H2O 0.025 mg/L, Na2EDTA 37.4 mg/L, FeSO4·7H2The mg/L of O 27.8, the mg/L of inositol 200, nicotinic acid 0.5
Mg/L, the mg/L of puridoxine hydrochloride 0.5, the mg/L of thiamine hydrochloride 1.0, the mg/L of glycine 2.0.
Embodiment 2：
（1）Explant is selected：Collection semi-lignified, red skin Qinggang spray raw then of robust growth are used as explant；
（2）Explant treatment：Explant is placed in 15 min are rinsed under running water, then remove explant blade, then by explant
Body is immersed in the mercuric chloride solution of volumetric concentration 0.1%, controls the min of soak time 20, finally cleans medicine with pure water, will
Explant is cut into band at least one axillary bud, and stem section 1.5-3cm long is standby depending in stem section degree of lignification classified placing container；
（3）Initial bud inducement cultivation：By step（2）The explant for obtaining is cultivated in being inoculated in initial bud inducement cultivation base, is placed in
Optical culture, the lx of illumination 1000, cultivate 30-35 d in the environment that 20 ± 1 DEG C of cultivation temperature, initial bud is sprouted, growth；Described
Initially the formula of bud inducement cultivation base is：The mg/L+KT 1.5 of 1/2 5.0 mg/L+NAA of improvement WPM culture mediums+6-BA 3.0
The mg/L+ folic acid 10mg/L+VB of 1.5 mg/L+ VC of mg/L+ZT 152 The g/L of 15 mg/+ agar, 3.0 g/L+ sucrose 25；
（4）Multiplying culture：Multiplying culture is carried out during the initial bud of height >=1cm is accessed into proliferated culture medium, optical culture, illumination is placed in
3000 lx, the h of daily illumination 16, in the environment of 25-28 DEG C of cultivation temperature, 35-40 d transfer once, move in circles, through 3-4
The culture in cycle, forms Multiple Buds；The formula of described proliferated culture medium is：The mg/L+ of improvement WPM culture mediums+6-BA 7.0
NAA 2.5 mg/L+ZT 1.0 mg/L+VC 15 mg/L+VB2 The g/L+ of 15 mg+L- Cys2s, 0 mg/L+ agar 3.6
The g/L of sucrose 30；
（5）Strong bud culture：By step（4）The Multiple Buds for obtaining are cut, 4-5 bud/clump, in insertion strong buds medium, are put
In optical culture, the lx of illumination 3000, the h of daily illumination 16 cultivate 35-40 d in the environment of 25-28 DEG C of cultivation temperature, form strong
Bud；The formula of described strong buds medium is：The mg/L+VC 15 of 3.5 mg/L+NAA of improvement WPM culture mediums+6-BA 1.0
mg/L+VB2 The g/L of 15 mg+L- Cys2s, 0 mg/L+ agar, 3.5 g/L+ sucrose 25；
（6）Culture of rootage：By step（5）In the simple bud insertion root media of the height >=2cm for obtaining, remaining budlet clump connects again
Plant in step（4）Proliferated culture medium in continue breed, then repeat step（5）, move in circles, obtain largely strong bud；It is raw
Root culture is placed in optical culture, the lx of illumination 3000, the h of daily illumination 16, root induction in the environment of 25-28 DEG C of cultivation temperature,
Rooting rate is 92%；The formula of described root media is：The mg/L+NAA 1.5 of 1/2 improvement WPM culture mediums+6-BA 2.0
The g/L of 3.5 g/L+ sucrose of mg/L+ agar 30.
The above component and envelope-bulk to weight ratio for improveing WPM culture mediums are：NH4NO3370mg/L, CaCl2·2H2O
82 mg/L, MgSO4·7H20 270 mg/L, KH2PO4170 mg/L, Ca (NO3)2·4H2O 180 mg/L, KI 0.83
Mg/L, MnSO4·H2O 22.4 mg/L, ZnSO4·7H2O 8.6 mg/L, Na2MoO4·2H2O 0.25 mg/L, CuSO4·
5H2O 0.025 mg/L, Na2EDTA 37.4 mg/L, FeSO4·7H2The mg/L of O 27.8, the mg/L of inositol 200, nicotinic acid 0.5
Mg/L, the mg/L of puridoxine hydrochloride 0.5, the mg/L of thiamine hydrochloride 1.0, the mg/L of glycine 2.0.
Embodiment 3：
（1）Explant is selected：Collection semi-lignified, red skin Qinggang spray raw then of robust growth are used as explant；
（2）Explant treatment：Explant is placed in 15 min are rinsed under running water, then remove explant blade, then by explant
Body is immersed in the mercuric chloride solution of volumetric concentration 0.1%, controls the min of soak time 20, finally cleans medicine with pure water, will
Explant is cut into band at least one axillary bud, and stem section 1.5-3cm long is standby depending in stem section degree of lignification classified placing container；
（3）Initial bud inducement cultivation：By step（2）The explant for obtaining is cultivated in being inoculated in initial bud inducement cultivation base, is placed in
Optical culture, the lx of illumination 1000, cultivate 30-35 d in the environment that 20 ± 1 DEG C of cultivation temperature, initial bud is sprouted, growth；Described
Initially the formula of bud inducement cultivation base is：The mg/L+KT 2.0 of 1/2 6.0 mg/L+NAA of improvement WPM culture mediums+6-BA 2.5
The mg/L+ folic acid 10mg/L+VB of 2.0 mg/L+ VC of mg/L+ZT 152 The g/L of 15 mg/+ agar, 3.0 g/L+ sucrose 25；
（4）Multiplying culture：Multiplying culture is carried out during the initial bud of height >=1cm is accessed into proliferated culture medium, optical culture, illumination is placed in
4000 lx, the h of daily illumination 16, in the environment of 25-28 DEG C of cultivation temperature, 35-40 d transfer once, move in circles, through 3-4
The culture in cycle, forms Multiple Buds；The formula of described proliferated culture medium is：The mg/L+ of improvement WPM culture mediums+6-BA 8.0
NAA 3.0 mg/L+ZT 1.0 mg/L+VC 15 mg/L+VB2 The g/L+ of 15 mg+L- Cys2s, 0 mg/L+ agar 3.6
The g/L of sucrose 30；
（5）Strong bud culture：By step（4）The Multiple Buds for obtaining are cut, 4-5 bud/clump, in insertion strong buds medium, are put
In optical culture, the lx of illumination 4000, the h of daily illumination 16 cultivate 35-40 d in the environment of 25-28 DEG C of cultivation temperature, form strong
Bud；The formula of described strong buds medium is：The mg/L+VC 15 of 4.0 mg/L+NAA of improvement WPM culture mediums+6-BA 1.5
mg/L+VB2 The g/L of 15 mg+L- Cys2s, 0 mg/L+ agar, 3.5 g/L+ sucrose 25；
（6）Culture of rootage：By step（5）In the simple bud insertion root media of the height >=2cm for obtaining, remaining budlet clump connects again
Plant in step（4）Proliferated culture medium in continue breed, then repeat step（5）, move in circles, obtain largely strong bud；It is raw
Root culture is placed in optical culture, the lx of illumination 4000, the h of daily illumination 16, root induction in the environment of 25-28 DEG C of cultivation temperature,
Rooting rate is 95%；；The formula of described root media is：The mg/L+NAA 1.5 of 1/2 improvement WPM culture mediums+6-BA 2.5
The g/L of 3.5 g/L+ sucrose of mg/L+ agar 30.
The above component and envelope-bulk to weight ratio for improveing WPM culture mediums are：NH4NO3370mg/L, CaCl2·2H2O
82 mg/L, MgSO4·7H20 270 mg/L, KH2PO4170 mg/L, Ca (NO3)2·4H2O 180 mg/L, KI 0.83
Mg/L, MnSO4·H2O 22.4 mg/L, ZnSO4·7H2O 8.6 mg/L, Na2MoO4·2H2O 0.25 mg/L, CuSO4·
5H2O 0.025 mg/L, Na2EDTA 37.4 mg/L, FeSO4·7H2The mg/L of O 27.8, the mg/L of inositol 200, nicotinic acid 0.5
Mg/L, the mg/L of puridoxine hydrochloride 0.5, the mg/L of thiamine hydrochloride 1.0, the mg/L of glycine 2.0.
Claims (7)
1. a kind of red skin Qinggang spray tissue culture propagation method, it is characterised in that：Selected including explant, explant is processed, initial
Bud inducement cultivation, Multiplying culture, strong bud culture and culture of rootage operation, collection semi-lignified, the red skin raw then of robust growth
Qinggang spray is pruned to explant, is inoculated in initial bud inducement cultivation base after sterilization treatment and obtains as explant
Take initial bud, then initial bud is inoculated in proliferated culture medium by 3-4 cycle proliferation culture, form Multiple Buds, will grow thickly
Bud is cultivated in accessing strong buds medium, finally by root induction, concrete operations step in the simple bud insertion root media of height >=2cm
It is rapid as follows：
（1）Explant is selected：Collection semi-lignified, red skin Qinggang spray raw then of robust growth are used as explant；
（2）Explant treatment：Explant is placed under running water and rinses 10-15 min, then remove explant blade, then will be outer
Implant is immersed in the mercuric chloride solution of volumetric concentration 0.1%, controls soak time 15-20 min, finally cleans medicine with pure water
Thing, band at least one axillary bud is cut into by explant, stem section 1.5-3cm long, depending in stem section degree of lignification classified placing container,
It is standby；
（3）Initial bud inducement cultivation：By step（2）The explant for obtaining is cultivated in being inoculated in initial bud inducement cultivation base, culture
30-35 d, initial bud is sprouted, growth；
（4）Multiplying culture：Multiplying culture, 35-40 d switchings one are carried out during the initial bud of height >=1cm is accessed into proliferated culture medium
It is secondary, move in circles, through the 3-4 culture in cycle, form Multiple Buds；
（5）Strong bud culture：By step（4）The Multiple Buds for obtaining are cut, 4-5 bud/clump, in insertion strong buds medium, training
35-40 d are supported, strong bud is formed；
（6）Culture of rootage：By step（5）Root induction in the simple bud insertion root media of the height >=2cm for obtaining；Remaining budlet
Cong Zaici is inoculated in step（4）Proliferated culture medium in continue breed, then repeat step（5）, move in circles, obtain a large amount of
Strong bud.
2. a kind of red skin Qinggang spray tissue culture propagation method according to claim 1, it is characterised in that：Described initial bud
The formula of inducing culture is：1/2 improvement WPM culture medium+6-BA 4.0-6.0 mg/L+NAA 2.0-3.0 mg/L+KT
The mg/L+ folic acid 10mg/L+VB of 1.0-2.0 mg/L+ZT 1.5-2.0 mg/L+ VC 152 The g/L+ sugarcanes of 15 mg/+ agar 3.0
25 g/L of sugar.
3. a kind of red skin Qinggang spray tissue culture propagation method according to claim 1, it is characterised in that：Described propagation training
Support base formula be：Improvement WPM culture medium+6-BA 6.0-8.0 mg/L+NAA 2.0-3.0 mg/L+ZT 0.5-1.0mg/L+
VC 15 mg/L+VB2 The g/L of 15 mg+L- Cys2s, 0 mg/L+ agar, 3.6 g/L+ sucrose 30.
4. a kind of red skin Qinggang spray tissue culture propagation method according to claim 1, it is characterised in that：Described strong bud training
Support base formula be：The mg/L+VB of improvement WPM culture medium+6-BA 3.0-4.0 mg/L+NAA 1.0-1.5 mg/L+VC 152
The g/L of 15 mg+L- Cys2s, 0 mg/L+ agar, 3.5 g/L+ sucrose 25.
5. a kind of red skin Qinggang spray tissue culture propagation method according to claim 1, it is characterised in that：Described training of taking root
Support base formula be：The g/L of 1/2 improvement WPM culture medium+6-BA 1.5-2.5 mg/L+NAA 1.0-1.5 mg/L+ agar 3.5
The g/L of+sucrose 30.
6. according to any described a kind of red skin Qinggang spray tissue culture propagation methods of claim 2-5, it is characterised in that：Described
Improve WPM culture mediums formula be：The above component and envelope-bulk to weight ratio for improveing WPM culture mediums are：NH4NO3 370mg/
L, CaCl2·2H2O 82 mg/L, MgSO4·7H20 270 mg/L, KH2PO4170 mg/L, Ca (NO3)2·4H2O 180
Mg/L, KI 0.83 mg/L, MnSO4·H2O 22.4 mg/L, ZnSO4·7H2O 8.6 mg/L, Na2MoO4·2H2O 0.25
Mg/L, CuSO4·5H2O 0.025 mg/L, Na2EDTA 37.4 mg/L, FeSO4·7H2The mg/L of O 27.8, inositol 200
Mg/L, the mg/L of nicotinic acid 0.5, the mg/L of puridoxine hydrochloride 0.5, the mg/L of thiamine hydrochloride 1.0, the mg/L of glycine 2.0.
7. a kind of red skin Qinggang spray tissue culture propagation method according to claim 1, it is characterised in that：Step（3）It is described
The cultivation control condition of initial bud inducement cultivation be：Optical culture, illumination 500-1000 lx, 20 ± 1 DEG C of cultivation temperature；Step
（4）Described Multiplying culture, step（5）Described strong bud culture, step（6）The cultivation control condition of described culture of rootage is equal
For：Optical culture, illumination 2500-4000 lx, the h of daily illumination 16,25-28 DEG C of cultivation temperature.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710079784.5A CN106718939A (en) | 2017-02-15 | 2017-02-15 | A kind of red skin Qinggang spray tissue culture propagation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710079784.5A CN106718939A (en) | 2017-02-15 | 2017-02-15 | A kind of red skin Qinggang spray tissue culture propagation method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106718939A true CN106718939A (en) | 2017-05-31 |
Family
ID=58957028
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710079784.5A Pending CN106718939A (en) | 2017-02-15 | 2017-02-15 | A kind of red skin Qinggang spray tissue culture propagation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106718939A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103004591A (en) * | 2012-11-29 | 2013-04-03 | 绵阳师范学院 | Fagus pashanica tissue culture rapid propagation method |
| CN104255480A (en) * | 2014-09-12 | 2015-01-07 | 南京通泽农业科技有限公司 | Rapid propagation method of cyclobalanopsis glauca |
| CN105830924A (en) * | 2016-04-12 | 2016-08-10 | 广西壮族自治区林业科学研究院 | A twig tissue culture propagation method for cinnamomum camphora (L.) presl |
-
2017
- 2017-02-15 CN CN201710079784.5A patent/CN106718939A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103004591A (en) * | 2012-11-29 | 2013-04-03 | 绵阳师范学院 | Fagus pashanica tissue culture rapid propagation method |
| CN104255480A (en) * | 2014-09-12 | 2015-01-07 | 南京通泽农业科技有限公司 | Rapid propagation method of cyclobalanopsis glauca |
| CN105830924A (en) * | 2016-04-12 | 2016-08-10 | 广西壮族自治区林业科学研究院 | A twig tissue culture propagation method for cinnamomum camphora (L.) presl |
Non-Patent Citations (1)
| Title |
|---|
| 李金华等: "湖南靖州特色种群赤皮青冈群落的研究", 《中南林业科技大学学报》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105638477A (en) | Rapid propagation method for dendrobium hancockii seeds | |
| CN102090329B (en) | Method for transplanting Damascus rose tissue culture seedling | |
| CN104145824A (en) | Method for cultivating subcultured bud of upright crown tissue culture seedling of fir wood | |
| CN103931497A (en) | Method for improving seedling rate of tissue culture seedlings of hylocereus undulatus britt | |
| CN103430845A (en) | Strawberry tissue culturing method | |
| CN104719158A (en) | Method for rapidly establishing medium-sized Chinese pennisetum herb tissue culture regeneration system by taking seeds as explants | |
| CN107211891B (en) | Konjak callus direct seeding and rapid propagation technology | |
| CN104137779A (en) | Method for regenerating sapium japonicum plant by inducing sapium japonicum stem rapidly | |
| CN102907326A (en) | Tissue culture propagation method for Medicagao Sativa L. | |
| CN106879464A (en) | A kind of chinquapin isolated culture plant strain regeneration method | |
| CN103283601B (en) | Method for rapidly propagating Hupeh fritillary by utilizing tissue culture technology | |
| CN102511390B (en) | Method and special culture medium for regenerating sterile induced plants of ormosia fordiana seeds | |
| CN101965799B (en) | Tissue culture quick propagation method for Opisthopappus Shih | |
| CN101904302B (en) | Method for somatic cell embryogeny and plant regeneration of medicinal plant schisandga chinensis baill | |
| CN104221854A (en) | Method for cultivating oncidium | |
| CN106718940A (en) | A kind of tissue culture and rapid propagation method suitable for tung oil tree | |
| CN102640656B (en) | Efficient seedling exercising transplanting method for raspberry tissue culture seedlings | |
| CN114041421A (en) | Tissue rapid propagation method of avocados | |
| CN111406647B (en) | Efficient starting culture medium for directly inducing tetraploid paulownia petioles to regenerate adventitious buds and application | |
| CN103430822A (en) | Aquaculture seed reproduction method for micro seed tubers of konjac | |
| CN106718939A (en) | A kind of red skin Qinggang spray tissue culture propagation method | |
| CN108719061B (en) | Method for inducing cherokee rose leaf to directly generate adventitious bud and regenerate plant | |
| CN112931226A (en) | Tissue culture and rapid propagation method for alnus orientalis | |
| CN107889745B (en) | Tissue culture method for preventing fir test-tube plantlet from deviating from crown | |
| CN101810141B (en) | Method for in-vitro rapid propagation of clivia miniata by using mature embryos as explants |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170531 |