CN103004591A - Fagus pashanica tissue culture rapid propagation method - Google Patents
Fagus pashanica tissue culture rapid propagation method Download PDFInfo
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- CN103004591A CN103004591A CN2012105250625A CN201210525062A CN103004591A CN 103004591 A CN103004591 A CN 103004591A CN 2012105250625 A CN2012105250625 A CN 2012105250625A CN 201210525062 A CN201210525062 A CN 201210525062A CN 103004591 A CN103004591 A CN 103004591A
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Abstract
The invention discloses a Fagus pashanica tissue culture rapid propagation method which comprises the following steps: picking Fagus pashanica branch buds as explants; peeling off all scales of the buds so as to expose the bud points; disinfecting for 5min in 0.1% of mercury bichloride; and inoculating in a culture medium with WPM+NAA (Naphthalene Acetic Acid) of 1.0mg/L, 6-BA of 2.0mg/L and carbon powder of 2.0mg/L. After the buds are inoculated for 15 days, leaves grow up, and after 45 days, the leaves expand and grow larger and the leaves are light green in color and grow exuberantly; after being continuously cultivated for 60 days, white root systems grow out from the roots, the hair roots are obvious, the root systems are developed, and complete plants are grown and can be acclimatized and transplanted. The method has the following benefits that firstly, a research material is single in resource and is accordant in clone genetic background; secondly, extremely fewer plant materials are used, so that the materials are easy to acquire with economy, convenience and high efficiency; thirdly, the cultivation condition is controllable, and anniversary test or production can be carried out; and fourthly, the Fagus pashanica grows rapidly with short cycle and strong repeatability; and fifthly, the management is convenient, and the automation control is facilitated.
Description
Technical field
What the present invention relates to is a kind of fagus pashannica tissue culture and rapid propagation method.
Background technology
Beech claims again fagus, is the common name to Angiospermae Fagaceae Fagus (Fagus L.) plant, and beech is worldwide extensive distribution seeds a: F.engleriana, and F.orientalis is connected the Eurasia with F.sylvatica; F.grandifolia and Nothofagus traverse southern hemisphere and northern hemisphere
[46]Beech is arbor or shrub; Bud has scale, and is microscler and sharp; Leaf comes off, and alternate has sawtooth; Hua Xianye is open; Male flower is lined up tool handle, sagging capitulum; Perianth mitriform, 5-7 splits; Stamen 8-16; It is right that female flower is generally, and is born in the involucre of armpit life, tool handle; Perianth 5-6 split and with the ovary symphysis; Ovary is the next, Room 3, style 3, fruit has 2 of nuts, contains in the involucre of one wooden, tool thorn or tool hump, neat 4 when ripe (seldom 3) lobe cracking; Nut ovum shape triangle, Chestnut has 3 carinate ribs, the top point; Plant the benevolence oil-containing.Its processed goods is exactly beech and the beech goods in the fashionable world.The Fagus plant origin is ancient, be found in the earliest quaternary glacier mid-term, reliable impression is just arranged to the third phase, be typical north temperate zone Floristic Components, geographical distribution Xi Jie in China, and as one of sign of dividing East Mid subtropical and western part, also be the important line of demarcation that China's Floristic Plant Geography distributes.The Fagus plant, about 11 kinds of the whole world, 6 kinds (fagus hayatae (Fagushaytae), long handle beech (Fagus longipetiolata), Fagus lucida (Fagus lucida), Fagus engleriana (Fagusengleriana), fagus pashannica and Pingwu beech (Fagus chienii)) of China, 5 kinds in continent.
Fagus pashannica is that three grades in Sichuan Province lays special stress on protecting seeds; be the trees novel species of finding on the dam, Shahe in large forest farm, Jiangkou first Sichuan Province Forestry surveying and designing institute professor of engineering Yang Qin week in August, 1975, be decided to be three grades in Sichuan Province by provincial government in May, 1987 and lay special stress on protecting seeds.This tree is planted has distribution on Beichuan, Nan Jiang and other places.
This species fruit size year phenomenon is obvious, gather difficulty, and natural germination rate is lower than 3%.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of fagus pashannica tissue culture and rapid propagation method for the deficiencies in the prior art.
Technical scheme of the present invention is as follows:
The fagus pashannica tissue culture and rapid propagation method, adopting the fagus pashannica twig is explant, and all scales of sprout are peelled off, and exposes the bud point, the 5min that sterilizes in 0.1% mercuric chloride is transferred in the WPM+NAA1.0mg/L+6-BA2.0mg/L+ carbon dust 2.0mg/L medium.Begin to grow after being seeded to 15d, the 45d rear blade has launched larger, and the leaf look light green, and it is vigorous to grow.After continuing to be cultured to 60d, base portion produces white root system, and hair root is obvious, and well developed root system forms whole plant, but acclimatization and transplants.
Beneficial effect of the present invention is as follows:
(1) the research material source is single, and clone genetic background is consistent;
(2) vegetable material that uses is few, and easily gathering economy is convenient, and efficient is high;
(3) condition of culture is controlled, but anniversary test or production;
(4) growth is fast, and the cycle is short, and repeatability is strong;
(5) convenient management is beneficial to automation control;
Embodiment
Below in conjunction with specific embodiment, the present invention is described in detail.
Adopting the fagus pashannica twig is explant, and all scales of sprout are peelled off, and exposes the bud point, and the 5min that sterilizes in 0.1% mercuric chloride is transferred in the WPM+NAA1.0mg/L+6-BA2.0mg/L+ carbon dust 2.0mg/L medium.Begin to grow after being seeded to 15d, the 45d rear blade has launched larger, and the leaf look light green, and it is vigorous to grow.After continuing to be cultured to 60d, base portion produces white root system, and hair root is obvious, and well developed root system forms whole plant, but acclimatization and transplants.
Should be understood that, for those of ordinary skills, can be improved according to the above description or conversion, and all these improvement and conversion all should belong to the protection domain of claims of the present invention.
Claims (1)
1. a fagus pashannica tissue culture and rapid propagation method is characterized in that, adopting the fagus pashannica twig is explant, all scales of sprout are peelled off, expose the bud point, the 5min that sterilizes in 0.1% mercuric chloride is transferred in the WPM+NAA1.0mg/L+6-BA2.0mg/L+ carbon dust 2.0mg/L medium; Begin to grow after being seeded to 15d, the 45d rear blade has launched larger, and the leaf look light green, and it is vigorous to grow; After continuing to be cultured to 60d, base portion produces white root system, and hair root is obvious, and well developed root system forms whole plant, but acclimatization and transplants.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012105250625A CN103004591A (en) | 2012-11-29 | 2012-11-29 | Fagus pashanica tissue culture rapid propagation method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2012105250625A CN103004591A (en) | 2012-11-29 | 2012-11-29 | Fagus pashanica tissue culture rapid propagation method |
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| CN103004591A true CN103004591A (en) | 2013-04-03 |
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| CN2012105250625A Pending CN103004591A (en) | 2012-11-29 | 2012-11-29 | Fagus pashanica tissue culture rapid propagation method |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106718939A (en) * | 2017-02-15 | 2017-05-31 | 唐春艳 | A kind of red skin Qinggang spray tissue culture propagation method |
| CN106718937A (en) * | 2017-02-14 | 2017-05-31 | 唐春艳 | The method that Qinggang tree obtains aseptic explant preparation and initial bud |
| CN106797888A (en) * | 2017-02-15 | 2017-06-06 | 唐春艳 | The rapid propagation method of Qinggang tree tissue cultures |
| CN106888966A (en) * | 2017-02-14 | 2017-06-27 | 唐春艳 | Red skin Qinggang aseptic explant is prepared and initial bud abductive approach |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070287633A1 (en) * | 2003-12-03 | 2007-12-13 | Weyerhaeuser Company | Use of a cytokinin to promote growth of shoots from a log |
| CN102657089A (en) * | 2012-05-12 | 2012-09-12 | 广西壮族自治区林业科学研究院 | Aseptic seeding method for zelkova trees |
| CN102792890A (en) * | 2012-08-03 | 2012-11-28 | 中南林业科技大学 | Rapid propagation method of tissue culture for zelkova schneideriana hand.-Mazz |
-
2012
- 2012-11-29 CN CN2012105250625A patent/CN103004591A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070287633A1 (en) * | 2003-12-03 | 2007-12-13 | Weyerhaeuser Company | Use of a cytokinin to promote growth of shoots from a log |
| CN102657089A (en) * | 2012-05-12 | 2012-09-12 | 广西壮族自治区林业科学研究院 | Aseptic seeding method for zelkova trees |
| CN102792890A (en) * | 2012-08-03 | 2012-11-28 | 中南林业科技大学 | Rapid propagation method of tissue culture for zelkova schneideriana hand.-Mazz |
Non-Patent Citations (1)
| Title |
|---|
| KARIN MEIER,ET AL.: "factors controlling micropropagation of mature fagus sylvatica", 《PLANT CELL,TISSUE AND ORGAN CULTURE》, vol. 39, 31 December 1994 (1994-12-31) * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106718937A (en) * | 2017-02-14 | 2017-05-31 | 唐春艳 | The method that Qinggang tree obtains aseptic explant preparation and initial bud |
| CN106888966A (en) * | 2017-02-14 | 2017-06-27 | 唐春艳 | Red skin Qinggang aseptic explant is prepared and initial bud abductive approach |
| CN106718939A (en) * | 2017-02-15 | 2017-05-31 | 唐春艳 | A kind of red skin Qinggang spray tissue culture propagation method |
| CN106797888A (en) * | 2017-02-15 | 2017-06-06 | 唐春艳 | The rapid propagation method of Qinggang tree tissue cultures |
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Application publication date: 20130403 |