CN108350474B - 小分子糖基化的方法 - Google Patents

小分子糖基化的方法 Download PDF

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CN108350474B
CN108350474B CN201680062080.0A CN201680062080A CN108350474B CN 108350474 B CN108350474 B CN 108350474B CN 201680062080 A CN201680062080 A CN 201680062080A CN 108350474 B CN108350474 B CN 108350474B
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sucrose phosphorylase
sucrose
range
phosphorylase
ascorbic acid
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CN108350474A (zh
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R·K·古迪明基
B·尼德茨基
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BASF Beauty Care Solutions France SAS
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/60—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/1066—Sucrose phosphate synthase (2.4.1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01007—Sucrose phosphorylase (2.4.1.7)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
CN201680062080.0A 2015-09-25 2016-09-22 小分子糖基化的方法 Active CN108350474B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP15186888.2 2015-09-25
EP15186888 2015-09-25
PCT/EP2016/072586 WO2017050920A1 (en) 2015-09-25 2016-09-22 Method for small molecule glycosylation

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CN108350474A CN108350474A (zh) 2018-07-31
CN108350474B true CN108350474B (zh) 2022-06-28

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US (1) US20180291410A1 (cg-RX-API-DMAC7.html)
EP (1) EP3353311B1 (cg-RX-API-DMAC7.html)
JP (1) JP7000327B2 (cg-RX-API-DMAC7.html)
KR (1) KR20180072705A (cg-RX-API-DMAC7.html)
CN (1) CN108350474B (cg-RX-API-DMAC7.html)
ES (1) ES2886572T3 (cg-RX-API-DMAC7.html)
WO (1) WO2017050920A1 (cg-RX-API-DMAC7.html)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108913641B (zh) * 2018-06-29 2021-02-02 浙江工业大学 一种重组大肠杆菌及其应用
CN109988778B (zh) * 2019-05-14 2020-10-27 南京工业大学 一种蔗糖磷酸化酶基因及其应用
CN111172128A (zh) * 2020-01-21 2020-05-19 浙江工业大学 一种蔗糖磷酸化酶在制备2-O-α-D-葡萄糖基-L-抗坏血酸中的应用
CN113980932B (zh) * 2021-07-15 2023-08-01 暨南大学 一种定点突变的α-葡萄糖苷酶
CN115786388B (zh) * 2022-12-26 2025-01-14 暨南大学 生产2-O-α-D-吡喃葡萄糖基-L-抗坏血酸的基因工程菌及其构建方法与应用
CN116144625A (zh) * 2023-02-24 2023-05-23 杭州佳嘉乐生物技术有限公司 重组蔗糖磷酸化酶及其催化糖基化反应的方法
CN120230742B (zh) * 2025-06-03 2025-07-29 山东三元生物科技股份有限公司 一种固定化细胞及其制备方法和2-O-α-D-吡喃葡萄糖基-L-抗坏血酸的制备方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1253022A (zh) * 1998-10-13 2000-05-17 强生消费者公司 提高局部用皮肤作用剂渗透性的组合物
CN103122341A (zh) * 2013-01-08 2013-05-29 江南大学 一种麦芽糊精底物特异性提高的环糊精糖基转移酶及其制备方法
WO2014060452A1 (en) * 2012-10-18 2014-04-24 Universiteit Gent A thermostable sucrose and sucrose-6'-phosphate phosphorylase
CN104039964A (zh) * 2011-03-25 2014-09-10 普罗特罗公司 可调控的基因表达系统及其构建体
CN104531629A (zh) * 2014-12-16 2015-04-22 江南大学 一种提高aa-2g转化率的环糊精葡萄糖基转移酶突变体

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2832848B2 (ja) 1989-10-21 1998-12-09 株式会社林原生物化学研究所 結晶２―Ｏ―α―Ｄ―グルコピラノシル―Ｌ―アスコルビン酸とその製造方法並びに用途
JP3134235B2 (ja) 1991-10-23 2001-02-13 株式会社林原生物化学研究所 ２−Ｏ−α−Ｄ−グルコピラノシル−Ｌ−アスコルビン酸高含有物の製造方法
JP4043312B2 (ja) 2002-08-06 2008-02-06 株式会社林原生物化学研究所 ２−Ｏ−α−Ｄ−グルコピラノシル−Ｌ−アスコルビン酸の製造方法

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1253022A (zh) * 1998-10-13 2000-05-17 强生消费者公司 提高局部用皮肤作用剂渗透性的组合物
CN104039964A (zh) * 2011-03-25 2014-09-10 普罗特罗公司 可调控的基因表达系统及其构建体
WO2014060452A1 (en) * 2012-10-18 2014-04-24 Universiteit Gent A thermostable sucrose and sucrose-6'-phosphate phosphorylase
CN103122341A (zh) * 2013-01-08 2013-05-29 江南大学 一种麦芽糊精底物特异性提高的环糊精糖基转移酶及其制备方法
CN104531629A (zh) * 2014-12-16 2015-04-22 江南大学 一种提高aa-2g转化率的环糊精葡萄糖基转移酶突变体

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Sucrose phosphorylase: a powerful transglucosylation catalyst for synthesis of α-D-glucosides as industrial fine chemicals;Goedl等;《Biocatalysis and Biotransformation》;20100101;第28卷(第1期);第10页第3段，表1，图1A *
Transglucosylation of ascorbic acid to ascorbic acid 2-glucoside by a recombinant sucrose phosphorylase from Bifidobacterium longum;Taeyeon Kwon等;《Biotechnol Lett》;20070415;第29卷;摘要，第612页最后一段 *

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JP7000327B2 (ja) 2022-02-04
ES2886572T3 (es) 2021-12-20
EP3353311A1 (en) 2018-08-01
KR20180072705A (ko) 2018-06-29
EP3353311B1 (en) 2021-06-16
JP2018535693A (ja) 2018-12-06
WO2017050920A1 (en) 2017-03-30
CN108350474A (zh) 2018-07-31
US20180291410A1 (en) 2018-10-11

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