CN107530654A - 通过在离散实体中条形码化对核酸进行测序 - Google Patents

通过在离散实体中条形码化对核酸进行测序 Download PDF

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Publication number
CN107530654A
CN107530654A CN201680020884.4A CN201680020884A CN107530654A CN 107530654 A CN107530654 A CN 107530654A CN 201680020884 A CN201680020884 A CN 201680020884A CN 107530654 A CN107530654 A CN 107530654A
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nucleic acid
discrete entities
bar code
code sequence
sequence
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Chinese (zh)
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A·R·阿贝特
J·哈里伯顿
F·兰
A·R·夏穆比
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University of California San Diego UCSD
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University of California San Diego UCSD
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Priority to CN202210828875.5A priority Critical patent/CN115011670A/zh
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Cited By (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110777048A (zh) * 2019-11-01 2020-02-11 上海市第六人民医院 一种高通量单外泌体分离装置
US10745762B2 (en) 2012-08-13 2020-08-18 The Regents Of The University Of California Method and system for synthesizing a target polynucleotide within a droplet
CN112005115A (zh) * 2018-02-12 2020-11-27 10X基因组学有限公司 表征来自单个细胞或细胞群体的多种分析物的方法
US11020736B2 (en) 2014-10-22 2021-06-01 The Regents Of The University Of California High definition microdroplet printer
WO2021128035A1 (zh) * 2019-12-25 2021-07-01 苏州绘真生物科技有限公司 高通量单细胞转录组测序方法及试剂盒
WO2021147069A1 (zh) * 2020-01-23 2021-07-29 青岛华大智造普惠科技有限公司 基于液滴微流控的单细胞测序及应用
US11111519B2 (en) 2015-02-04 2021-09-07 The Regents Of The University Of California Sequencing of nucleic acids via barcoding in discrete entities
US11124830B2 (en) 2016-12-21 2021-09-21 The Regents Of The University Of California Single cell genomic sequencing using hydrogel based droplets
US11142791B2 (en) 2016-08-10 2021-10-12 The Regents Of The University Of California Combined multiple-displacement amplification and PCR in an emulsion microdroplet
CN113795591A (zh) * 2019-04-02 2021-12-14 使命生物公司 表征肿瘤并且识别肿瘤异质性的方法和系统
CN113891961A (zh) * 2019-07-12 2022-01-04 深圳华大生命科学研究院 一种全基因组全流程微流控自动化建库方法和装置
CN114073995A (zh) * 2020-08-13 2022-02-22 安行生物技术有限公司 分析单细胞的微流控设备和单元及其方法
US11312990B2 (en) 2014-06-27 2022-04-26 The Regents Of The University Of California PCR-activated sorting (PAS)
CN114568027A (zh) * 2019-08-05 2022-05-31 使命生物公司 用于确定细胞轨迹的单细胞分析的方法和设备
US11365441B2 (en) 2019-05-22 2022-06-21 Mission Bio, Inc. Method and apparatus for simultaneous targeted sequencing of DNA, RNA and protein
CN114729393A (zh) * 2019-11-15 2022-07-08 美天施生物科技有限两合公司 用于单细胞索引的颜色和条码化珠
WO2022148311A1 (zh) * 2021-01-06 2022-07-14 南京诺唯赞生物科技股份有限公司 多靶点蛋白质-dna相互作用的研究方法和工具
CN115461469A (zh) * 2019-12-12 2022-12-09 癌症研究技术有限公司 空间条形码化
CN115867676A (zh) * 2020-05-29 2023-03-28 国立研究开发法人理化学研究所 细胞群的处理方法和细胞群中所含的基因的分析方法
US11667954B2 (en) 2019-07-01 2023-06-06 Mission Bio, Inc. Method and apparatus to normalize quantitative readouts in single-cell experiments
US11781129B2 (en) 2017-10-18 2023-10-10 Mission Bio, Inc. Method, systems and apparatus for single cell analysis

Families Citing this family (211)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
ES2663234T3 (es) 2012-02-27 2018-04-11 Cellular Research, Inc Composiciones y kits para recuento molecular
WO2013177560A1 (en) 2012-05-25 2013-11-28 The Regents Of The University Of California Microfluidic systems for particle trapping and separation
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN104769127A (zh) 2012-08-14 2015-07-08 10X基因组学有限公司 微胶囊组合物及方法
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
WO2014093676A1 (en) 2012-12-14 2014-06-19 10X Technologies, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN108753766A (zh) 2013-02-08 2018-11-06 10X基因组学有限公司 多核苷酸条形码生成
EP2986741B1 (en) 2013-04-17 2019-07-24 Agency For Science, Technology And Research Method for generating extended sequence reads
LT3013984T (lt) 2013-06-25 2023-06-12 Prognosys Biosciences, Inc. Biologinių taikinių erdvinių modelių mėginyje nustatymo būdai
EP3480321B8 (en) 2013-08-28 2021-03-10 Becton, Dickinson and Company Massively parallel single cell analysis
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
CN106413896B (zh) 2014-04-10 2019-07-05 10X基因组学有限公司 用于封装和分割试剂的流体装置、系统和方法及其应用
US20150376700A1 (en) 2014-06-26 2015-12-31 10X Genomics, Inc. Analysis of nucleic acid sequences
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
CN118910216A (zh) 2014-06-26 2024-11-08 10X基因组学有限公司 分析来自单个细胞或细胞群体的核酸的方法
GB201414177D0 (en) * 2014-08-11 2014-09-24 Sphere Fluidics Ltd Droplet Sorting
CA2964472A1 (en) 2014-10-29 2016-05-06 10X Genomics, Inc. Methods and compositions for targeted nucleic acid sequencing
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
US10900065B2 (en) 2014-11-14 2021-01-26 University Of Washington Methods and kits for labeling cellular molecules
AU2016207023B2 (en) 2015-01-12 2019-12-05 10X Genomics, Inc. Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
WO2016137973A1 (en) 2015-02-24 2016-09-01 10X Genomics Inc Partition processing methods and systems
CN107532202A (zh) 2015-02-24 2018-01-02 10X 基因组学有限公司 用于靶向核酸序列覆盖的方法
CN120425026A (zh) 2015-04-10 2025-08-05 十程基因技术瑞典公司 生物样本的空间区别、多重核酸分析
CN107849560B (zh) * 2015-04-27 2021-10-22 阿布维特罗有限责任公司 对治疗剂和疾病特异性抗原进行测序、确定、配对和验证的方法
GB201509640D0 (en) * 2015-06-03 2015-07-15 Sphere Fluidics Ltd Systems and methods
HK1259411A1 (zh) * 2015-08-17 2019-11-29 加利福尼亚大学董事会 基於微滴的多重置换扩增(mda)方法及相关组合物
US9862941B2 (en) 2015-10-14 2018-01-09 Pioneer Hi-Bred International, Inc. Single cell microfluidic device
ES2876039T3 (es) * 2015-11-10 2021-11-11 Illumina Inc Generación inercial de gotitas y encapsulación de partículas
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
SG11201804086VA (en) 2015-12-04 2018-06-28 10X Genomics Inc Methods and compositions for nucleic acid analysis
JP6735348B2 (ja) 2016-02-11 2020-08-05 １０エックス ジェノミクス， インコーポレイテッド 全ゲノム配列データのデノボアセンブリのためのシステム、方法及び媒体
WO2018183796A1 (en) * 2017-03-31 2018-10-04 Predicine, Inc. Systems and methods for predicting and monitoring cancer therapy
WO2017181161A1 (en) 2016-04-15 2017-10-19 Predicine, Inc. Systems and methods for detecting genetic alterations
WO2017197343A2 (en) 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
EP3472351B1 (de) 2016-06-15 2020-10-28 Ludwig-Maximilians-Universität München Einzelmolekülnachweis bzw. -quantifizierung durch dna-nanotechnologie
US10830545B2 (en) 2016-07-12 2020-11-10 Fractal Heatsink Technologies, LLC System and method for maintaining efficiency of a heat sink
JP6853523B2 (ja) * 2016-07-27 2021-03-31 国立大学法人京都大学 ヘリカーゼを用いたｐｃｒ
US10858649B2 (en) 2016-09-15 2020-12-08 Augmenta Bioworks, Inc. Immune repertoire sequence amplification methods and applications
US11174503B2 (en) 2016-09-21 2021-11-16 Predicine, Inc. Systems and methods for combined detection of genetic alterations
EP3516400B1 (en) 2016-09-26 2023-08-16 Becton, Dickinson and Company Measurement of protein expression using reagents with barcoded oligonucleotide sequences
WO2018067792A1 (en) * 2016-10-07 2018-04-12 President And Fellows Of Harvard College Sequencing of bacteria or other species
WO2018071448A1 (en) * 2016-10-11 2018-04-19 The Regents Of The University Of California Systems and methods to encapsulate and preserve organic matter for analysis
US11479806B2 (en) 2016-11-10 2022-10-25 Takara Bio Usa, Inc. Methods of producing amplified double stranded deoxyribonucleic acids and compositions and kits for use therein
KR102534408B1 (ko) * 2016-11-16 2023-05-18 카탈로그 테크놀로지스, 인크. 핵산-기반 데이터 저장
WO2018096058A1 (en) * 2016-11-23 2018-05-31 Universite De Strasbourg Tandem barcoding of target molecules for their absolute quantification at single-entity resolution
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) * 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2018140966A1 (en) 2017-01-30 2018-08-02 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
EP4218738B1 (en) * 2017-02-24 2024-10-16 The Regents of The University of California Particle-drop structures and methods for making and using the same
CN107083423B (zh) * 2017-03-27 2022-01-28 北京极客基因科技有限公司 一种药物靶点预测和药物全方面评价方法
US12054764B2 (en) 2017-04-14 2024-08-06 The Broad Institute, Inc. High-throughput screens for exploring biological functions of microscale biological systems
US10544413B2 (en) 2017-05-18 2020-01-28 10X Genomics, Inc. Methods and systems for sorting droplets and beads
WO2018213643A1 (en) 2017-05-18 2018-11-22 10X Genomics, Inc. Methods and systems for sorting droplets and beads
US10400235B2 (en) 2017-05-26 2019-09-03 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
SG11201901822QA (en) 2017-05-26 2019-03-28 10X Genomics Inc Single cell analysis of transposase accessible chromatin
MA49352A (fr) * 2017-05-26 2020-04-08 Abvitro Llc Séquençage de bibliothèque de polynucléotides à haut rendement et analyse de transcriptome
CN110719959B (zh) 2017-06-05 2021-08-06 贝克顿迪金森公司 针对单细胞的样品索引
US11517901B2 (en) 2017-06-09 2022-12-06 The Regents Of The University Of California High-efficiency particle encapsulation in droplets with particle spacing and downstream droplet sorting
WO2018227210A1 (en) 2017-06-09 2018-12-13 The Regents Of The University Of California High-efficiency encapsulation in droplets based on hydrodynamic vortices control
WO2018236918A1 (en) * 2017-06-20 2018-12-27 Bio-Rad Laboratories, Inc. MDA USING A BALL OLIGONUCLEOTIDE
TWI637834B (zh) * 2017-07-11 2018-10-11 台灣奈米碳素股份有限公司 微流道裝置的製造方法及其結構
EP3655718A4 (en) 2017-07-17 2021-03-17 Alexander Poltorak MULTIFRACTAL HEAT SINK SYSTEM AND PROCESS
GB2581599B8 (en) * 2017-08-10 2023-09-20 Element Biosciences Inc Tagging nucleic acid molecules from single cells for phased sequencing
US10610865B2 (en) 2017-08-22 2020-04-07 10X Genomics, Inc. Droplet forming devices and system with differential surface properties
DK3684949T3 (en) 2017-09-22 2024-07-08 Univ Washington In situ combinatorial labeling of cellular molecules
EP4462123A3 (en) * 2017-09-27 2025-02-12 Bio-Rad Laboratories, Inc. Digital affinity linkage assay
AU2018401700B2 (en) 2017-09-29 2024-05-02 The Regents Of The University Of California Method of generating monodisperse emulsions
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
WO2019075409A1 (en) 2017-10-12 2019-04-18 The Regents Of The University Of California ISOLATION AND IDENTIFICATION WITHOUT MICROFLUIDIC LABEL OF CELLS USING FLUORESCENCE LIFE IMAGING (FLIM)
US20190112655A1 (en) * 2017-10-18 2019-04-18 Mission Bio, Inc. Method, Systems and Apparatus for High-Throughput Single-Cell DNA Sequencing With Droplet Microfluidics
US11499127B2 (en) 2017-10-20 2022-11-15 The Regents Of The University Of California Multi-layered microfluidic systems for in vitro large-scale perfused capillary networks
US11745179B2 (en) 2017-10-20 2023-09-05 The Regents Of The University Of California Microfluidic systems and methods for lipoplex-mediated cell transfection
WO2019084055A1 (en) * 2017-10-23 2019-05-02 Massachusetts Institute Of Technology CLASSIFICATION OF GENETIC VARIATION FROM UNICELLULAR TRANSCRIPTOMS
WO2019083852A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
EP3700672B1 (en) 2017-10-27 2022-12-28 10X Genomics, Inc. Methods for sample preparation and analysis
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019104337A1 (en) 2017-11-27 2019-05-31 The Trustees Of Columbia University In The City Of New York Rna printing and sequencing devices, methods, and systems
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
WO2019113235A1 (en) * 2017-12-06 2019-06-13 10X Genomics, Inc. Methods and systems for processing nucleic acid molecules
EP3724658A1 (en) 2017-12-12 2020-10-21 10X Genomics, Inc. Systems and methods for single cell processing
WO2019126789A1 (en) 2017-12-22 2019-06-27 10X Genomics, Inc. Systems and methods for processing nucleic acid molecules from one or more cells
CN111556900A (zh) * 2017-12-22 2020-08-18 赛默飞世尔科技波罗的海封闭股份公司 包括胺的聚合酶链反应组合物
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
WO2019169028A1 (en) * 2018-02-28 2019-09-06 10X Genomics, Inc. Transcriptome sequencing through random ligation
CN120425022A (zh) * 2018-03-02 2025-08-05 量子细胞公司 从组织中分离和表达分析感兴趣的区域的方法、组成和装置
EP3762494B1 (en) * 2018-03-07 2022-11-23 Wisconsin Alumni Research Foundation High throughput nucleic acid profiling of single cells
CN112262218B (zh) 2018-04-06 2024-11-08 10X基因组学有限公司 用于单细胞处理中的质量控制的系统和方法
KR102657606B1 (ko) 2018-04-20 2024-04-15 일루미나, 인코포레이티드 단일 세포를 캡슐화하는 방법, 캡슐화된 세포 및 이의 용도
AU2019262048B2 (en) 2018-05-03 2025-09-04 Becton, Dickinson And Company High throughput multiomics sample analysis
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
EP3797162A1 (en) * 2018-05-22 2021-03-31 Université de Strasbourg Absolute quantification of target molecules at single-entity resolution using tandem barcoding
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
WO2020009998A1 (en) * 2018-07-03 2020-01-09 Chan Zuckerberg Biohub, Inc. Method to perform high-throughput single cell genomic and phenotypic analyses
WO2020010258A1 (en) * 2018-07-05 2020-01-09 Accuragen Holdings Limited Compositions and methods for digital polymerase chain reaction
US12188014B1 (en) 2018-07-25 2025-01-07 10X Genomics, Inc. Compositions and methods for nucleic acid processing using blocking agents
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
WO2020028882A1 (en) 2018-08-03 2020-02-06 10X Genomics, Inc. Methods and systems to minimize barcode exchange
WO2020028912A2 (en) 2018-08-03 2020-02-06 Catolog Technologies, Inc Systems and methods for storing and reading nucleic acid-based data with error protection
US20200048626A1 (en) * 2018-08-07 2020-02-13 Augmenta Bioworks, Inc. Methods for Making Gel Beads and Core and Shell Beads with a Cell
US20210301247A1 (en) * 2018-08-10 2021-09-30 Cellino Biotech, Inc. System for image-driven cell manufacturing
US20210346888A1 (en) * 2018-08-17 2021-11-11 The Regents Of The University Of California Monodispersed Particle-Triggered Droplet Formation from Stable Jets
US12065688B2 (en) * 2018-08-20 2024-08-20 10X Genomics, Inc. Compositions and methods for cellular processing
WO2020041148A1 (en) 2018-08-20 2020-02-27 10X Genomics, Inc. Methods and systems for detection of protein-dna interactions using proximity ligation
WO2020072380A1 (en) 2018-10-01 2020-04-09 Cellular Research, Inc. Determining 5' transcript sequences
US11662341B2 (en) 2018-10-10 2023-05-30 Augmenta Bioworks, Inc. Methods for isolating immune binding proteins
US11608524B1 (en) * 2018-10-25 2023-03-21 Wisconsin Alumni Research Foundation Methods of analyzing cells
MY202795A (en) * 2018-10-26 2024-05-22 Illumina Inc Modulating polymer beads for dna processing
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
CN113767176A (zh) * 2018-12-10 2021-12-07 10X基因组学有限公司 成像系统硬件
EP3894593B1 (en) 2018-12-13 2024-10-02 DNA Script Direct oligonucleotide synthesis on cdna
CN111378557B (zh) 2018-12-26 2023-06-06 财团法人工业技术研究院 用于产生液珠的管状结构及液珠产生方法
US20220033811A1 (en) * 2018-12-28 2022-02-03 Biobloxx Ab Method and kit for preparing complementary dna
EP3906318A1 (en) * 2019-01-06 2021-11-10 10X Genomics, Inc. Methods and systems for enrichment of barcodes
US12169198B2 (en) 2019-01-08 2024-12-17 10X Genomics, Inc. Systems and methods for sample analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
JP2022518917A (ja) * 2019-01-22 2022-03-17 ミッション バイオ インコーポレイテッド 核酸の検出方法及びプライマーの設計方法
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
US12305239B2 (en) 2019-02-12 2025-05-20 10X Genomics, Inc. Analysis of nucleic acid sequences
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
US11584953B2 (en) 2019-02-12 2023-02-21 10X Genomics, Inc. Methods for processing nucleic acid molecules
US12275993B2 (en) 2019-02-12 2025-04-15 10X Genomics, Inc. Analysis of nucleic acid sequences
WO2020167866A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transposon loading
WO2020167862A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transfer of reagents between droplets
EP3924506A1 (en) 2019-02-14 2021-12-22 Becton Dickinson and Company Hybrid targeted and whole transcriptome amplification
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
WO2020183591A1 (en) * 2019-03-11 2020-09-17 Enplas Corporation Fluid handling method, and fluid handling device and fluid handling system used for the method
CN113767178A (zh) 2019-03-11 2021-12-07 10X基因组学有限公司 用于处理光学标签化珠粒的系统和方法
AU2020253585A1 (en) * 2019-04-02 2021-11-18 Mission Bio, Inc. Methods, systems, and apparatus for nucleic acid detection
EP3983558A4 (en) * 2019-06-12 2023-06-28 Ultima Genomics, Inc. Methods for accurate base calling using molecular barcodes
US20230212556A1 (en) * 2019-06-27 2023-07-06 Cell Microsystems, Inc. Systems and methods for associating single cell imaging with rna transcriptomics
US12186751B2 (en) 2019-06-28 2025-01-07 10X Genomics, Inc. Devices and systems incorporating acoustic ordering and methods of use thereof
CN114051534B (zh) 2019-07-22 2025-02-21 贝克顿迪金森公司 单细胞染色质免疫沉淀测序测定
MX2022001324A (es) * 2019-07-31 2022-05-19 Bioskryb Genomics Inc Análisis de células individuales.
US12179199B2 (en) 2019-08-09 2024-12-31 The Regents Of The University Of California Microfluidic single-cell pairing array for studying cell-cell interactions in isolated compartments
CA3150030A1 (en) * 2019-09-04 2021-03-11 Marion Francis SETLIFF Methods for identification of antigen binding specificity of antibodies
US12235262B1 (en) 2019-09-09 2025-02-25 10X Genomics, Inc. Methods and systems for single cell protein analysis
WO2021067286A1 (en) * 2019-09-30 2021-04-08 Shield Diagnostics Corp. Devices for determining a target nucleic acid profile and methods of use thereof
EP4037815A4 (en) * 2019-10-05 2024-01-24 Mission Bio, Inc. METHODS, SYSTEMS AND APPARATUS ASSOCIATED WITH COPY NUMBER VARIATIONS AND SINGLE NUCLEOTIDE VARIATIONS DETECTED SIMULTANEOUSLY IN SINGLE CELLS
US11919000B2 (en) 2019-10-10 2024-03-05 1859, Inc. Methods and systems for microfluidic screening
US20220381781A1 (en) * 2019-10-16 2022-12-01 Georgia Tech Research Corporation Methods for ultrasensitive detection of protein and cellular biomarkers
US11773436B2 (en) 2019-11-08 2023-10-03 Becton, Dickinson And Company Using random priming to obtain full-length V(D)J information for immune repertoire sequencing
US12059679B2 (en) * 2019-11-19 2024-08-13 10X Genomics, Inc. Methods and devices for sorting droplets and particles
US12163189B2 (en) 2019-11-27 2024-12-10 University Of Washington Method for preparation and high-throughput microbial single-cell RNA sequencing of bacteria
CN115038794A (zh) 2019-12-23 2022-09-09 10X基因组学有限公司 在基于分区的测定中使用固定生物样品的组合物和方法
US11649497B2 (en) 2020-01-13 2023-05-16 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and RNA
AU2021207581A1 (en) 2020-01-13 2022-08-11 Fluent Biosciences Inc. Methods and systems for single cell gene profiling
EP4090739A4 (en) 2020-01-13 2024-02-14 Fluent Biosciences Inc. Single cell sequencing
WO2021146187A1 (en) 2020-01-13 2021-07-22 Fluent Biosciences Inc. Emulsion based drug screening
US20230045126A1 (en) * 2020-01-14 2023-02-09 President And Fellows Of Harvard College Devices and methods for determining nucleic acids using digital droplet pcr and related techniques
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
EP4093884A4 (en) 2020-01-24 2024-05-15 Vanderbilt University Methods for identification of ligand-blocking antibodies and for determining antibody potency
US12188010B2 (en) 2020-01-29 2025-01-07 Becton, Dickinson And Company Barcoded wells for spatial mapping of single cells through sequencing
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
US12449419B1 (en) 2020-02-12 2025-10-21 10X Genomics, Inc. Methods for detecting binding of peptide-MHC monomers to T cells
EP4111168A1 (en) 2020-02-25 2023-01-04 Becton Dickinson and Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
US11866782B2 (en) 2020-03-16 2024-01-09 Fluent Biosciences Inc. Multi-omic analysis in monodisperse droplets
CN111454942A (zh) * 2020-03-16 2020-07-28 张晓鲁 一种同一样品的转录组和基因组同时建立测序文库的构建方法
CN115768884A (zh) 2020-03-20 2023-03-07 使命生物公司 用于全基因组扩增的单细胞工作流程
US12428685B2 (en) 2020-03-24 2025-09-30 Illumina, Inc. Viral detection using template emulsification
CN111394432B (zh) * 2020-03-27 2023-03-24 深圳闪量科技有限公司 基于通用探针芯片的多重定量pcr检测系统
EP4143333A1 (en) * 2020-04-30 2023-03-08 Dimensiongen Devices and methods for macromolecular manipulation
GB202007059D0 (en) * 2020-05-13 2020-06-24 Univ Oxford Innovation Ltd Polynucleotide arrays
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
CN115605614A (zh) 2020-05-14 2023-01-13 贝克顿迪金森公司（Us） 用于免疫组库谱分析的引物
EP4414459B1 (en) 2020-05-22 2025-09-03 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
US12265079B1 (en) 2020-06-02 2025-04-01 10X Genomics, Inc. Systems and methods for detecting analytes from captured single biological particles
EP4158055B1 (en) 2020-06-02 2024-03-27 Becton, Dickinson and Company Oligonucleotides and beads for 5 prime gene expression assay
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
US12241059B2 (en) 2020-07-15 2025-03-04 Illumina, Inc. Tiered ligation oligos
US12391940B2 (en) 2020-07-31 2025-08-19 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
GB202012730D0 (en) * 2020-08-14 2020-09-30 Cambridge Entpr Ltd Modular microfluidic devices, systems and methodds for total RNA analyses
US12084715B1 (en) 2020-11-05 2024-09-10 10X Genomics, Inc. Methods and systems for reducing artifactual antisense products
US12480158B1 (en) 2020-11-05 2025-11-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2022099208A1 (en) * 2020-11-09 2022-05-12 The General Hospital Corporation High throughput microfluidics for analysis of immune cell activation
EP4244379A1 (en) * 2020-11-13 2023-09-20 10X Genomics, Inc. Nano-partitions (encapsulated nucleic acid processing enzymes) for cell-lysis and multiple reactions in partition-based assays
US11739443B2 (en) 2020-11-20 2023-08-29 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
US20220243264A1 (en) * 2020-11-23 2022-08-04 The Broad Institute, Inc. Systems and methods for amplifying rna
CN116685850A (zh) 2020-12-15 2023-09-01 贝克顿迪金森公司 单细胞分泌组分析
US12398262B1 (en) 2021-01-22 2025-08-26 10X Genomics, Inc. Triblock copolymer-based cell stabilization and fixation system and methods of use thereof
CN114836838A (zh) * 2021-02-01 2022-08-02 南方医科大学 一种中通量单细胞拷贝数文库构建的方法及其应用
AU2022227563A1 (en) 2021-02-23 2023-08-24 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
AU2022258444A1 (en) 2021-04-14 2023-11-23 Partillion Bioscience Corporation Nanoscale reaction chambers and methods of using the same
EP4330678A1 (en) * 2021-04-26 2024-03-06 Ecole Polytechnique Federale De Lausanne (Epfl) High complexity microcompartment-based interaction screening
US20240376523A1 (en) * 2021-09-14 2024-11-14 Becton, Dickinson And Company Full length single cell rna sequencing
WO2023154816A1 (en) * 2022-02-09 2023-08-17 Mission Bio, Inc. Systems and methods of detecting merged droplets in single cell sequencing
DK181862B1 (en) * 2022-03-21 2025-02-24 Samplix Aps Method and kit for targeted enrichment of large dna molecules for long-read sequencing using facs or microfluidic partitioning
EP4514961A1 (en) * 2022-04-28 2025-03-05 Bioskryb Genomics, Inc. Single cell multiomics
US20240035071A1 (en) 2022-06-17 2024-02-01 10X Genomics, Inc. Catalytic de-crosslinking of samples for in situ analysis
WO2024097393A1 (en) * 2022-11-04 2024-05-10 Fluid Discovery Systems, compositions, and methods for single cell analysis
WO2024163490A1 (en) * 2023-01-30 2024-08-08 Idexx Laboratories, Inc. Methods, systems, and device for electrowetting bar-coded magnetic beads
WO2025040497A1 (en) * 2023-08-18 2025-02-27 Qiagen Gmbh Method for improving nucleic acid processing
CN120783873B (zh) * 2025-07-09 2025-12-05 上海锦测医学检验所有限公司 一种智能化蛋白质组学数据异常检测与分析方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110059556A1 (en) * 2009-09-04 2011-03-10 The Research Foundation Of State University Of New York Rapid and Continuous Analyte Processing in Droplet Microfluidic Devices
WO2012109600A2 (en) * 2011-02-11 2012-08-16 Raindance Technologies, Inc. Methods for forming mixed droplets
US20120220494A1 (en) * 2011-02-18 2012-08-30 Raindance Technolgies, Inc. Compositions and methods for molecular labeling
US20140323316A1 (en) * 2013-03-15 2014-10-30 Complete Genomics, Inc. Multiple tagging of individual long dna fragments
US20140378349A1 (en) * 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing

Family Cites Families (118)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU5733994A (en) 1992-11-30 1994-06-22 Wellcome Foundation Limited, The Sequential targeting of tumor sites with oligonucleotide conjugates of antibody and complementary oligonucleotide conjugates of chelated radionuclide
US20020068357A1 (en) 1995-09-28 2002-06-06 Mathies Richard A. Miniaturized integrated nucleic acid processing and analysis device and method
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
US6319507B1 (en) 1997-05-02 2001-11-20 Kobo Products, Inc. Agar gel bead composition and method
WO1999002671A1 (en) 1997-07-07 1999-01-21 Medical Research Council In vitro sorting method
US6436677B1 (en) 2000-03-02 2002-08-20 Promega Corporation Method of reverse transcription
US7041208B2 (en) 2001-12-11 2006-05-09 Sau Lan Tang Staats Microfluidic devices and methods for two-dimensional separations
JP2006507921A (ja) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体分散のための方法および装置
EP1594981B1 (en) 2003-01-29 2010-03-17 454 Corporation Double ended sequencing
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
DE10339452A1 (de) * 2003-08-22 2005-03-17 Institut für Physikalische Hochtechnologie e.V. Vorrichtung und Verfahren zur Strukturierung von Flüssigkeiten
WO2005021151A1 (en) 2003-08-27 2005-03-10 President And Fellows Of Harvard College Electronic control of fluidic species
EP1664328A2 (en) 2003-09-26 2006-06-07 Circleamp, Inc. Amplification of polynucleotides by rolling circle amplification
EP1691792A4 (en) 2003-11-24 2008-05-28 Yeda Res & Dev COMPOSITIONS AND PROCESSES FOR SORTING IN VITRO OF MOLECULE AND CELL LIBRARIES
KR100647282B1 (ko) 2003-12-24 2006-11-17 삼성전자주식회사 ＤＮＡ 마이크로어레이 스폿팅(Ｓｐｏｔｉｎｇ) 장치 및이를 이용한 스폿팅 방법
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
US20060201868A1 (en) 2005-03-11 2006-09-14 Simmons Blake A Methods and devices for high-throughput dielectrophoretic concentration
US20070141593A1 (en) 2005-08-22 2007-06-21 Lee Linda G Apparatus, system, and method using immiscible-fluid-discrete-volumes
US8383059B2 (en) 2005-09-30 2013-02-26 University Of Utah Research Foundation Microfluidic interface for highly parallel addressing of sensing arrays
US9183349B2 (en) 2005-12-16 2015-11-10 Nextbio Sequence-centric scientific information management
US20100137163A1 (en) 2006-01-11 2010-06-03 Link Darren R Microfluidic Devices and Methods of Use in The Formation and Control of Nanoreactors
EP2495337A1 (en) * 2006-02-24 2012-09-05 Callida Genomics, Inc. High throughput genome sequencing on DNA arrays
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US20080014589A1 (en) 2006-05-11 2008-01-17 Link Darren R Microfluidic devices and methods of use thereof
CA2653321A1 (en) * 2006-05-26 2007-12-06 Althea Technologies, Inc. Biochemical analysis of partitioned cells
AU2007255110B8 (en) 2006-06-02 2014-04-10 Pfizer Products Inc. Circulating tumor cell assay
EP2415872B1 (en) 2006-12-14 2016-05-11 Dow AgroSciences LLC Optimized non-canonical zinc finger proteins
US8338166B2 (en) 2007-01-04 2012-12-25 Lawrence Livermore National Security, Llc Sorting, amplification, detection, and identification of nucleic acid subsequences in a complex mixture
WO2008109176A2 (en) 2007-03-07 2008-09-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
US8454906B2 (en) 2007-07-24 2013-06-04 The Regents Of The University Of California Microfabricated droplet generator for single molecule/cell genetic analysis in engineered monodispersed emulsions
US8268564B2 (en) 2007-09-26 2012-09-18 President And Fellows Of Harvard College Methods and applications for stitched DNA barcodes
GB0720202D0 (en) * 2007-10-16 2007-11-28 Cambridge Entpr Ltd Microfluidic systems
US9138700B2 (en) 2008-01-18 2015-09-22 The Regents Of The University Of California Accurate and rapid micromixer for integrated microfluidic devices
JP5737707B2 (ja) 2008-03-04 2015-06-17 クリスタル バイオサイエンス インク．Ｃｒｙｓｔａｌ Ｂｉｏｓｃｉｅｎｃｅ Ｉｎｃ． ゲル状マイクロドロップ組成物およびその使用方法
US9409177B2 (en) 2008-03-21 2016-08-09 Lawrence Livermore National Security, Llc Chip-based device for parallel sorting, amplification, detection, and identification of nucleic acid subsequences
US8852952B2 (en) 2008-05-03 2014-10-07 Advanced Liquid Logic, Inc. Method of loading a droplet actuator
US8709762B2 (en) 2010-03-02 2014-04-29 Bio-Rad Laboratories, Inc. System for hot-start amplification via a multiple emulsion
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US20130084572A1 (en) 2011-09-30 2013-04-04 Quantalife, Inc. Calibrations and controls for droplet-based assays
JP5457222B2 (ja) 2009-02-25 2014-04-02 エフ．ホフマン−ラ ロシュ アーゲー 小型化ハイスループット核酸分析
EP2977455B1 (en) 2009-06-15 2020-04-15 Complete Genomics, Inc. Method for long fragment read sequencing
EP4019977B1 (en) 2009-06-26 2024-11-20 President and Fellows of Harvard College Fluid injection
CA3106547C (en) 2009-09-02 2022-07-05 Bio-Rad Laboratories, Inc. System for mixing fluids by coalescence of multiple emulsions
US8746285B2 (en) 2009-09-04 2014-06-10 Auburn University Programmable fluidic droplet generation
US9376713B2 (en) 2009-09-23 2016-06-28 The Board Of Trustees Of The University Of Illinois Label free detection of nucleic acid amplification
EP2957641B1 (en) 2009-10-15 2017-05-17 Ibis Biosciences, Inc. Multiple displacement amplification
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
US20120270739A1 (en) 2010-01-19 2012-10-25 Verinata Health, Inc. Method for sample analysis of aneuploidies in maternal samples
AU2013203624B2 (en) 2010-02-09 2015-06-25 General Electric Company Isothermal amplification of nucleic acid using a mixture of randomized primers and specific primers
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
EP2550528B1 (en) 2010-03-25 2019-09-11 Bio-Rad Laboratories, Inc. Droplet generation for droplet-based assays
CA2767114A1 (en) 2010-03-25 2011-09-29 Bio-Rad Laboratories, Inc. Droplet transport system for detection
JP5573335B2 (ja) 2010-04-28 2014-08-20 株式会社島津製作所 磁性体粒子操作デバイス及び磁性体粒子操作方法
WO2011154042A1 (en) 2010-06-10 2011-12-15 Albert-Ludwigs-Universität Freiburg Apparatus and method for dispensing cells or particles confined in a free flying droplet
US9650629B2 (en) 2010-07-07 2017-05-16 Roche Molecular Systems, Inc. Clonal pre-amplification in emulsion
EP2595754B1 (en) 2010-07-22 2018-04-04 Gencell Biosystems Limited Composite liquid cells
WO2012027366A2 (en) 2010-08-23 2012-03-01 President And Fellows Of Harvard College Acoustic waves in microfluidics
WO2012045012A2 (en) 2010-09-30 2012-04-05 Raindance Technologies, Inc. Sandwich assays in droplets
EP2436444A1 (en) 2010-10-01 2012-04-04 Centre National de la Recherche Scientifique (C.N.R.S.) Microfluidic device for production and collection of droplets of a fluid
ES2966040T3 (es) 2010-10-08 2024-04-18 Harvard College Alto rendimiento de células individuales con código de barra
US20130123121A1 (en) 2010-11-22 2013-05-16 The University Of Chicago Methods and/or Use of Oligonucleotide-Bead Conjugates for Assays and Detections
DK2652155T3 (en) 2010-12-16 2017-02-13 Gigagen Inc Methods for Massive Parallel Analysis of Nucleic Acids in Single Cells
US20130295587A1 (en) 2011-01-24 2013-11-07 Ge Healthcare Bio-Sciences Ab Method of coupling binding agents to a substrate surface
US8765455B2 (en) 2011-01-27 2014-07-01 Lawrence Livermore National Security, Llc Chip-based droplet sorting
EP2670863B1 (en) * 2011-01-31 2018-06-27 H. Hoffnabb-La Roche Ag Methods of identifying multiple epitopes in cells
EP2670894B1 (en) 2011-02-02 2017-11-29 University Of Washington Through Its Center For Commercialization Massively parallel continguity mapping
WO2012142213A2 (en) 2011-04-15 2012-10-18 The Johns Hopkins University Safe sequencing system
EP2702175B1 (en) 2011-04-25 2018-08-08 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
SG10201605049QA (en) 2011-05-20 2016-07-28 Fluidigm Corp Nucleic acid encoding reactions
WO2012162296A2 (en) 2011-05-23 2012-11-29 President And Fellows Of Harvard College Control of emulsions, including multiple emulsions
WO2012167142A2 (en) * 2011-06-02 2012-12-06 Raindance Technolgies, Inc. Enzyme quantification
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
US20130189700A1 (en) 2011-07-25 2013-07-25 Bio-Rad Laboratories, Inc. Breakage of an emulsion containing nucleic acid
WO2013015793A1 (en) 2011-07-26 2013-01-31 Verinata Health, Inc. Method for determining the presence or absence of different aneuploidies in a sample
US20130032235A1 (en) 2011-08-02 2013-02-07 Teledyne Dalsa Semiconductor, Inc. Integrated microfluidic check valve and device including such a check valve
US9469874B2 (en) * 2011-10-18 2016-10-18 The Regents Of The University Of California Long-range barcode labeling-sequencing
WO2013095469A1 (en) 2011-12-21 2013-06-27 Intel Corporation Psmi using at-speed scan capture
BR112014019323A8 (pt) 2012-02-08 2017-07-11 Harvard College Formação de gotícula com uso de decomposição de fluido
US9176031B2 (en) 2012-02-24 2015-11-03 Raindance Technologies, Inc. Labeling and sample preparation for sequencing
ES2776673T3 (es) 2012-02-27 2020-07-31 Univ North Carolina Chapel Hill Métodos y usos para etiquetas moleculares
WO2013134261A1 (en) * 2012-03-05 2013-09-12 President And Fellows Of Harvard College Systems and methods for epigenetic sequencing
ES2899687T3 (es) * 2012-05-14 2022-03-14 Irepertoire Inc Método para aumentar la precisión en la detección cuantitativa de polinucleótidos
US10161007B2 (en) 2012-08-13 2018-12-25 The Regents Of The University Of California Methods and systems for detecting biological components
US20140378345A1 (en) 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
CN104769127A (zh) 2012-08-14 2015-07-08 10X基因组学有限公司 微胶囊组合物及方法
WO2014039912A1 (en) 2012-09-07 2014-03-13 Bio-Rad Laboratories, Inc. Compositions, systems and methods for droplet formation, spacing and detection
WO2014047556A1 (en) 2012-09-21 2014-03-27 The Broad Institute, Inc. Compositions and methods for long insert, paired end libraries of nucleic acids in emulsion droplets
CA2888042A1 (en) 2012-11-27 2014-06-05 Gencell Biosystems Ltd. Handling liquid samples
WO2014093676A1 (en) 2012-12-14 2014-06-19 10X Technologies, Inc. Methods and systems for processing polynucleotides
WO2014108323A1 (en) 2013-01-10 2014-07-17 Eth Zurich Method and apparatus for depositing droplets onto a substrate
WO2014138132A2 (en) 2013-03-04 2014-09-12 Verax Biomedical Incorporated Multi-analyte assay
WO2014153071A1 (en) 2013-03-14 2014-09-25 The Broad Institute, Inc. Methods for quantitating dna using digital multiple displacement amplification
US9752179B2 (en) * 2013-03-14 2017-09-05 Cold Spring Harbor Laboratory Trans-splicing transcriptome profiling
US10119134B2 (en) * 2013-03-15 2018-11-06 Abvitro Llc Single cell bar-coding for antibody discovery
WO2014151658A1 (en) 2013-03-15 2014-09-25 The Regents Of The University Of California High-speed on demand microfluidic droplet generation and manipulation
DE102013207900A1 (de) 2013-04-30 2014-10-30 Volkswagen Ag Membran-Elektroden-Einheit und Brennstoffzelle mit einer solchen
EP3480321B8 (en) 2013-08-28 2021-03-10 Becton, Dickinson and Company Massively parallel single cell analysis
WO2015069798A1 (en) 2013-11-05 2015-05-14 The Regents Of The University Of California Single-cell forensic short tandem repeat typing within microfluidic droplets
WO2015120398A1 (en) 2014-02-10 2015-08-13 Gencell Biosystems Limited Composite liquid cell (clc) mediated nucleic acid library preparation device, and methods for using the same
CN113215221A (zh) 2014-04-08 2021-08-06 华盛顿大学商业中心 用于使用多分散小滴执行数字检定的方法和设备
US20150298091A1 (en) 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
DK3134536T3 (da) 2014-04-21 2020-02-03 Harvard College Systemer og fremgangsmåder til at stregkodemarkere nukleinsyrer
CN106574925B (zh) 2014-05-23 2019-07-26 萤火虫生物股份有限公司 用于生物测定的底物介导的反应器
DK3155119T3 (en) 2014-06-11 2018-12-17 Samplix Aps NUCLEOTIDE SEQUENCE EXCLUSION ENHANCEMENT BY DROP SORTING (NEEDLS)
CN118910216A (zh) * 2014-06-26 2024-11-08 10X基因组学有限公司 分析来自单个细胞或细胞群体的核酸的方法
US10697007B2 (en) 2014-06-27 2020-06-30 The Regents Of The University Of California PCR-activated sorting (PAS)
WO2016064755A2 (en) 2014-10-20 2016-04-28 The Regents Of The University Of Califronia Rapid modulation of droplet composition with membrane microvalves
CN107107058B (zh) 2014-10-22 2021-08-10 加利福尼亚大学董事会 高清晰度微液滴打印机
WO2016126871A2 (en) 2015-02-04 2016-08-11 The Regents Of The University Of California Sequencing of nucleic acids via barcoding in discrete entities
CA2974299A1 (en) 2015-02-04 2016-08-11 The Regents Of The University Of California Multiple-emulsion nucleic acid amplification
HK1259411A1 (zh) 2015-08-17 2019-11-29 加利福尼亚大学董事会 基於微滴的多重置换扩增(mda)方法及相关组合物
US20190127789A1 (en) 2016-04-19 2019-05-02 President And Fellows Of Harvard College Immobilization-based systems and methods for genetic analysis and other applications
EP3497228A4 (en) 2016-08-10 2020-05-27 The Regents of The University of California MULTI-DISPLACEMENT AMPLIFICATION AND PCR COMBINED IN AN EMULSION MICRO DROPLET
AU2017382905A1 (en) 2016-12-21 2019-07-04 The Regents Of The University Of California Single cell genomic sequencing using hydrogel based droplets
EP3559272B1 (en) 2017-11-17 2022-08-31 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110059556A1 (en) * 2009-09-04 2011-03-10 The Research Foundation Of State University Of New York Rapid and Continuous Analyte Processing in Droplet Microfluidic Devices
WO2012109600A2 (en) * 2011-02-11 2012-08-16 Raindance Technologies, Inc. Methods for forming mixed droplets
US20120220494A1 (en) * 2011-02-18 2012-08-30 Raindance Technolgies, Inc. Compositions and methods for molecular labeling
US20140378349A1 (en) * 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
US20140323316A1 (en) * 2013-03-15 2014-10-30 Complete Genomics, Inc. Multiple tagging of individual long dna fragments

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US11781129B2 (en) 2017-10-18 2023-10-10 Mission Bio, Inc. Method, systems and apparatus for single cell analysis
CN112005115A (zh) * 2018-02-12 2020-11-27 10X基因组学有限公司 表征来自单个细胞或细胞群体的多种分析物的方法
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