CA2974306A1 - Sequencing of nucleic acids via barcoding in discrete entities - Google Patents

Sequencing of nucleic acids via barcoding in discrete entities Download PDF

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Publication number
CA2974306A1
CA2974306A1 CA2974306A CA2974306A CA2974306A1 CA 2974306 A1 CA2974306 A1 CA 2974306A1 CA 2974306 A CA2974306 A CA 2974306A CA 2974306 A CA2974306 A CA 2974306A CA 2974306 A1 CA2974306 A1 CA 2974306A1
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Canada
Prior art keywords
nucleic acid
discrete
acid barcode
target molecules
sequences
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Pending
Application number
CA2974306A
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English (en)
French (fr)
Inventor
Adam R. Abate
John HALIBURTON
Freeman LAN
Adam R. SCIAMBI
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University of California, San Diego
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University of California, San Diego
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Application filed by University of California, San Diego filed Critical University of California, San Diego
Publication of CA2974306A1 publication Critical patent/CA2974306A1/en
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
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CA2974306A 2015-02-04 2016-02-03 Sequencing of nucleic acids via barcoding in discrete entities Pending CA2974306A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562112075P 2015-02-04 2015-02-04
US62/112,075 2015-02-04
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Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10745762B2 (en) 2012-08-13 2020-08-18 The Regents Of The University Of California Method and system for synthesizing a target polynucleotide within a droplet
US11020736B2 (en) 2014-10-22 2021-06-01 The Regents Of The University Of California High definition microdroplet printer
US11111519B2 (en) 2015-02-04 2021-09-07 The Regents Of The University Of California Sequencing of nucleic acids via barcoding in discrete entities
US11124830B2 (en) 2016-12-21 2021-09-21 The Regents Of The University Of California Single cell genomic sequencing using hydrogel based droplets
US11142791B2 (en) 2016-08-10 2021-10-12 The Regents Of The University Of California Combined multiple-displacement amplification and PCR in an emulsion microdroplet
US11312990B2 (en) 2014-06-27 2022-04-26 The Regents Of The University Of California PCR-activated sorting (PAS)
US11365441B2 (en) 2019-05-22 2022-06-21 Mission Bio, Inc. Method and apparatus for simultaneous targeted sequencing of DNA, RNA and protein
US11667954B2 (en) 2019-07-01 2023-06-06 Mission Bio, Inc. Method and apparatus to normalize quantitative readouts in single-cell experiments
US11781129B2 (en) 2017-10-18 2023-10-10 Mission Bio, Inc. Method, systems and apparatus for single cell analysis
EP4424824A3 (en) * 2016-11-16 2024-11-27 Catalog Technologies, Inc. Nucleic acid-based data storage
US12437841B2 (en) 2018-08-03 2025-10-07 Catalog Technologies, Inc. Systems and methods for storing and reading nucleic acid-based data with error protection
US12708886B2 (en) 2018-09-28 2026-08-18 The Regents Of The University Of California Method of generating monodisperse emulsions

Families Citing this family (234)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
EP3321378B1 (en) 2012-02-27 2021-11-10 Becton, Dickinson and Company Compositions for molecular counting
US10564147B2 (en) 2012-05-25 2020-02-18 The Regents Of The University Of California Microfluidic systems for particle trapping and separation using cavity acoustic transducers
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) * 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN114891871A (zh) 2012-08-14 2022-08-12 10X基因组学有限公司 微胶囊组合物及方法
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP3567116A1 (en) 2012-12-14 2019-11-13 10X Genomics, Inc. Methods and systems for processing polynucleotides
KR20230003659A (ko) 2013-02-08 2023-01-06 10엑스 제노믹스, 인크. 폴리뉴클레오티드 바코드 생성
SG10201708498VA (en) 2013-04-17 2017-11-29 Agency Science Tech & Res Method for generating extended sequence reads
CN111500680B (zh) 2013-06-25 2024-04-16 普罗格诺西斯生物科学公司 检测样品中生物靶标的空间分布的方法和系统
CN105705659B (zh) 2013-08-28 2019-11-29 贝克顿迪金森公司 大规模平行单细胞分析
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
US9694361B2 (en) 2014-04-10 2017-07-04 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
EP4574974A3 (en) 2014-06-26 2025-10-08 10x Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
KR20170026383A (ko) 2014-06-26 2017-03-08 10엑스 제노믹스, 인크. 핵산 서열의 분석
GB201414177D0 (en) * 2014-08-11 2014-09-24 Sphere Fluidics Ltd Droplet Sorting
JP2017532042A (ja) 2014-10-29 2017-11-02 １０エックス ゲノミクス，インコーポレイテッド 標的化核酸配列決定のための方法及び組成物
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
US10900065B2 (en) 2014-11-14 2021-01-26 University Of Washington Methods and kits for labeling cellular molecules
AU2016207023B2 (en) 2015-01-12 2019-12-05 10X Genomics, Inc. Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
EP4286516A3 (en) 2015-02-24 2024-03-06 10X Genomics, Inc. Partition processing methods and systems
AU2016222719B2 (en) 2015-02-24 2022-03-31 10X Genomics, Inc. Methods for targeted nucleic acid sequence coverage
EP4321627A3 (en) 2015-04-10 2024-04-17 10x Genomics Sweden AB Spatially distinguished, multiplex nucleic acid analysis of biological specimens
CN107849560B (zh) * 2015-04-27 2021-10-22 阿布维特罗有限责任公司 对治疗剂和疾病特异性抗原进行测序、确定、配对和验证的方法
WO2016191533A1 (en) 2015-05-26 2016-12-01 The Trustees Of Columbia University In The City Of New York Rna printing and sequencing devices, methods, and systems
GB201509640D0 (en) * 2015-06-03 2015-07-15 Sphere Fluidics Ltd Systems and methods
CN108350488A (zh) * 2015-08-17 2018-07-31 加利福尼亚大学董事会 基于微滴的多重置换扩增(mda)方法及相关组合物
US9862941B2 (en) 2015-10-14 2018-01-09 Pioneer Hi-Bred International, Inc. Single cell microfluidic device
ES2876039T3 (es) * 2015-11-10 2021-11-11 Illumina Inc Generación inercial de gotitas y encapsulación de partículas
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
KR102723865B1 (ko) 2015-12-04 2024-10-29 10엑스 제노믹스, 인크. 핵산 분석을 위한 방법 및 조성물
JP6735348B2 (ja) 2016-02-11 2020-08-05 １０エックス ジェノミクス， インコーポレイテッド 全ゲノム配列データのデノボアセンブリのためのシステム、方法及び媒体
WO2018057820A1 (en) 2016-09-21 2018-03-29 Predicine, Inc. Systems and methods for combined detection of genetic alterations
WO2017181161A1 (en) 2016-04-15 2017-10-19 Predicine, Inc. Systems and methods for detecting genetic alterations
WO2018183796A1 (en) * 2017-03-31 2018-10-04 Predicine, Inc. Systems and methods for predicting and monitoring cancer therapy
WO2017184707A1 (en) 2016-04-19 2017-10-26 President And Fellows Of Harvard College Immobilization-based systems and methods for genetic analysis and other applications
WO2017197343A2 (en) 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
WO2017216270A1 (de) 2016-06-15 2017-12-21 Grabmayr Heinrich Einzelmolekülnachweis bzw. -quantifizierung durch dna-nanotechnologie
EP3485215B1 (en) 2016-07-12 2023-06-07 Alexander Poltorak System and method for maintaining efficiency of a heat sink
JP6853523B2 (ja) * 2016-07-27 2021-03-31 国立大学法人京都大学 ヘリカーゼを用いたｐｃｒ
CA3032528A1 (en) 2016-09-15 2018-03-22 Augmenta Bioworks, Inc. Immune repertoire sequence amplification methods and applications
IL265478B2 (en) 2016-09-26 2024-10-01 Becton Dickinson Co Measurement of protein expression using reagents with barcoded oligonucleotide sequences
WO2018067792A1 (en) * 2016-10-07 2018-04-12 President And Fellows Of Harvard College Sequencing of bacteria or other species
US11090653B2 (en) 2016-10-11 2021-08-17 The Regents Of The University Of California Systems and methods to encapsulate and preserve organic matter for analysis
WO2018089550A1 (en) 2016-11-10 2018-05-17 Takara Bio Usa, Inc. Methods of producing amplified double stranded deoxyribonucleic acids and compositions and kits for use therein
CN110114473A (zh) * 2016-11-23 2019-08-09 斯特拉斯堡大学 靶分子的串联条形码添加以便以单实体分辨率对靶分子进行绝对定量
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) * 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
EP4310183B1 (en) 2017-01-30 2025-07-09 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
WO2018145041A1 (en) 2017-02-06 2018-08-09 10X Genomics, Inc. Systems and methods for nucleic acid preparation
DK4218738T3 (da) * 2017-02-24 2024-11-11 Univ California Partikeldråbestrukturer og fremgangsmåder til fremstilling og anvendelse af disse
CN107083423B (zh) * 2017-03-27 2022-01-28 北京极客基因科技有限公司 一种药物靶点预测和药物全方面评价方法
US12054764B2 (en) 2017-04-14 2024-08-06 The Broad Institute, Inc. High-throughput screens for exploring biological functions of microscale biological systems
EP3625353B1 (en) 2017-05-18 2022-11-30 10X Genomics, Inc. Methods and systems for sorting droplets and beads
US12049667B2 (en) * 2017-05-26 2024-07-30 Abvitro Llc High-throughput polynucleotide library sequencing and transcriptome analysis
CN116064732A (zh) 2017-05-26 2023-05-05 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
JP7536450B2 (ja) 2017-06-05 2024-08-20 ベクトン・ディキンソン・アンド・カンパニー 単一細胞用のサンプルインデックス付加
US11517901B2 (en) 2017-06-09 2022-12-06 The Regents Of The University Of California High-efficiency particle encapsulation in droplets with particle spacing and downstream droplet sorting
WO2018227210A1 (en) 2017-06-09 2018-12-13 The Regents Of The University Of California High-efficiency encapsulation in droplets based on hydrodynamic vortices control
US11186862B2 (en) 2017-06-20 2021-11-30 Bio-Rad Laboratories, Inc. MDA using bead oligonucleotide
TWI637834B (zh) * 2017-07-11 2018-10-11 台灣奈米碳素股份有限公司 微流道裝置的製造方法及其結構
CN111433549A (zh) 2017-07-17 2020-07-17 分形散热器技术有限责任公司 多重分形散热器系统及方法
EP3665280A4 (en) * 2017-08-10 2021-10-06 Metabiotech Corporation MARKING OF NUCLEIC ACID MOLECULES FROM SINGLE CELLS FOR PHASE-CONTROLLED SEQUENCING
US10549279B2 (en) 2017-08-22 2020-02-04 10X Genomics, Inc. Devices having a plurality of droplet formation regions
DK3684949T3 (en) 2017-09-22 2024-07-08 Univ Washington In situ combinatorial labeling of cellular molecules
CN111164221B (zh) 2017-09-27 2024-03-29 生物辐射实验室股份有限公司 数字化亲和连接试验
JP2020535951A (ja) 2017-09-29 2020-12-10 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 単分散エマルションを生成する方法
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
WO2019075409A1 (en) 2017-10-12 2019-04-18 The Regents Of The University Of California ISOLATION AND IDENTIFICATION WITHOUT MICROFLUIDIC LABEL OF CELLS USING FLUORESCENCE LIFE IMAGING (FLIM)
WO2019079640A1 (en) * 2017-10-18 2019-04-25 Mission Bio, Inc. METHOD, SYSTEMS, AND DEVICE FOR HIGH-EFFICIENCY UNICELLULAR DNA SEQUENCING OF DROPLET MICROFLUIDS
WO2019079787A1 (en) 2017-10-20 2019-04-25 The Regents Of The University Of California MICROFLUIDIC SYSTEMS AND METHODS FOR CELL TRANSFECTION VIA LIPOFLEX
US11499127B2 (en) 2017-10-20 2022-11-15 The Regents Of The University Of California Multi-layered microfluidic systems for in vitro large-scale perfused capillary networks
US11732257B2 (en) * 2017-10-23 2023-08-22 Massachusetts Institute Of Technology Single cell sequencing libraries of genomic transcript regions of interest in proximity to barcodes, and genotyping of said libraries
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
WO2019083852A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING
CN111479631B (zh) 2017-10-27 2022-02-22 10X基因组学有限公司 用于样品制备和分析的方法和系统
EP3625361A1 (en) 2017-11-15 2020-03-25 10X Genomics, Inc. Functionalized gel beads
WO2019099908A1 (en) 2017-11-17 2019-05-23 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
EP3717661A1 (en) 2017-11-27 2020-10-07 The Trustees of Columbia University in the City of New York Rna printing and sequencing devices, methods, and systems
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
WO2019113235A1 (en) * 2017-12-06 2019-06-13 10X Genomics, Inc. Methods and systems for processing nucleic acid molecules
CN118818037A (zh) * 2017-12-12 2024-10-22 10X基因组学有限公司 用于单细胞处理的系统和方法
WO2019126789A1 (en) 2017-12-22 2019-06-27 10X Genomics, Inc. Systems and methods for processing nucleic acid molecules from one or more cells
WO2019122200A1 (en) * 2017-12-22 2019-06-27 Thermo Fisher Scientific Baltics Uab Polymerase chain reaction composition comprising amines
CA3089756A1 (en) 2018-01-29 2019-08-01 St. Jude Children's Research Hospital, Inc. Method for nucleic acid amplification
EP3752832A1 (en) 2018-02-12 2020-12-23 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
WO2019169028A1 (en) * 2018-02-28 2019-09-06 10X Genomics, Inc. Transcriptome sequencing through random ligation
KR20250099416A (ko) * 2018-03-02 2025-07-01 퀀텀사이트, 인크. 조직으로부터 관심 영역의 단리 및 발현 분석을 위한 방법, 조성물, 및 장치
WO2019173460A1 (en) * 2018-03-07 2019-09-12 Wisconsin Alumni Research Foundation High throughput nucleic acid profiling of single cells
EP3775271B1 (en) 2018-04-06 2025-03-12 10X Genomics, Inc. Systems and methods for quality control in single cell processing
CA3067181A1 (en) 2018-04-20 2019-10-24 Illumina, Inc. Methods of encapsulating single cells, the encapsulated cells and uses thereof
CA3097976A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company High throughput multiomics sample analysis
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
US20210214791A1 (en) * 2018-05-22 2021-07-15 Universite De Strasbourg Absolute quantification of target molecules at single-entity resolution using tandem barcoding
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
US12227741B2 (en) 2018-07-03 2025-02-18 Cz Biohub Sf, Llc Method to perform high-throughput single cell genomic and phenotypic analyses
CN112654713A (zh) * 2018-07-05 2021-04-13 安可济控股有限公司 用于数字聚合酶链反应的组合物和方法
US12188014B1 (en) 2018-07-25 2025-01-07 10X Genomics, Inc. Compositions and methods for nucleic acid processing using blocking agents
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
US12163179B2 (en) 2018-08-03 2024-12-10 10X Gemomics, Inc. Methods and systems to minimize barcode exchange
US20200048626A1 (en) * 2018-08-07 2020-02-13 Augmenta Bioworks, Inc. Methods for Making Gel Beads and Core and Shell Beads with a Cell
WO2020033871A1 (en) * 2018-08-10 2020-02-13 Cellino Biotech, Inc. System for image-driven cell manufacturing
AU2019321552A1 (en) 2018-08-17 2021-03-11 The Regents Of The University Of California Monodispersed particle-triggered droplet formation from stable jets
WO2020041148A1 (en) 2018-08-20 2020-02-27 10X Genomics, Inc. Methods and systems for detection of protein-dna interactions using proximity ligation
US12065688B2 (en) * 2018-08-20 2024-08-20 10X Genomics, Inc. Compositions and methods for cellular processing
EP3861134B1 (en) 2018-10-01 2024-09-04 Becton, Dickinson and Company Determining 5' transcript sequences
WO2020077017A2 (en) 2018-10-10 2020-04-16 Augmenta Bioworks, Inc. Methods for isolating immune binding proteins
US11608524B1 (en) * 2018-10-25 2023-03-21 Wisconsin Alumni Research Foundation Methods of analyzing cells
MY202795A (en) * 2018-10-26 2024-05-22 Illumina Inc Modulating polymer beads for dna processing
EP3894585A2 (en) * 2018-12-10 2021-10-20 10X Genomics, Inc. Generating capture probes for spatial analysis
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
SG11202105441WA (en) 2018-12-13 2021-06-29 Dna Script Direct oligonucleotide synthesis on cells and biomolecules
TWI725686B (zh) 2018-12-26 2021-04-21 財團法人工業技術研究院 用於產生液珠的管狀結構及液珠產生方法
WO2020136438A1 (en) * 2018-12-28 2020-07-02 Biobloxx Ab Method and kit for preparing complementary dna
CN113874521B (zh) * 2019-01-06 2024-11-01 10X基因组学有限公司 用于富集条形码的方法和系统
US12169198B2 (en) 2019-01-08 2024-12-17 10X Genomics, Inc. Systems and methods for sample analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
WO2020154391A1 (en) * 2019-01-22 2020-07-30 Mission Bio Methods of nucleic acid detection and primer design
US12305239B2 (en) 2019-02-12 2025-05-20 10X Genomics, Inc. Analysis of nucleic acid sequences
WO2020167862A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transfer of reagents between droplets
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
WO2020167866A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transposon loading
US12275993B2 (en) 2019-02-12 2025-04-15 10X Genomics, Inc. Analysis of nucleic acid sequences
WO2020168013A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Methods for processing nucleic acid molecules
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
CN113454234B (zh) 2019-02-14 2025-03-18 贝克顿迪金森公司 杂合体靶向和全转录物组扩增
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
SG11202111242PA (en) 2019-03-11 2021-11-29 10X Genomics Inc Systems and methods for processing optically tagged beads
WO2020183591A1 (en) * 2019-03-11 2020-09-17 Enplas Corporation Fluid handling method, and fluid handling device and fluid handling system used for the method
EP3947722A1 (en) 2019-03-27 2022-02-09 10X Genomics, Inc. Systems and methods for processing rna from cells
AU2020253585A1 (en) * 2019-04-02 2021-11-18 Mission Bio, Inc. Methods, systems, and apparatus for nucleic acid detection
EP3947673A4 (en) * 2019-04-02 2023-01-11 Mission Bio, Inc. METHODS AND SYSTEMS FOR PROTEOMIC PROFILING AND CHARACTERIZATION
EP3983558A4 (en) * 2019-06-12 2023-06-28 Ultima Genomics, Inc. Methods for accurate base calling using molecular barcodes
WO2020264387A1 (en) * 2019-06-27 2020-12-30 Cell Microsystems, Inc. Systems and methods for associating single cell imaging with rna transcriptomics
US12186751B2 (en) 2019-06-28 2025-01-07 10X Genomics, Inc. Devices and systems incorporating acoustic ordering and methods of use thereof
CN113891961A (zh) * 2019-07-12 2022-01-04 深圳华大生命科学研究院 一种全基因组全流程微流控自动化建库方法和装置
WO2021016239A1 (en) 2019-07-22 2021-01-28 Becton, Dickinson And Company Single cell chromatin immunoprecipitation sequencing assay
US20230220377A1 (en) * 2019-07-31 2023-07-13 BioSkryb Genomics, Inc. Single cell analysis
JP2022543278A (ja) 2019-08-05 2022-10-11 ミッション バイオ インコーポレイテッド 細胞軌道を測定するためのシングルセル分析のための方法及び装置
US12179199B2 (en) 2019-08-09 2024-12-31 The Regents Of The University Of California Microfluidic single-cell pairing array for studying cell-cell interactions in isolated compartments
US20220315982A1 (en) * 2019-09-04 2022-10-06 Vanderbilt University Methods for identification of antigen binding specificity of antibodies
US12235262B1 (en) 2019-09-09 2025-02-25 10X Genomics, Inc. Methods and systems for single cell protein analysis
WO2021067286A1 (en) * 2019-09-30 2021-04-08 Shield Diagnostics Corp. Devices for determining a target nucleic acid profile and methods of use thereof
WO2021067966A1 (en) * 2019-10-05 2021-04-08 Mission Bio, Inc. Methods, systems and apparatus for copy number variations and single nucleotide variations simultaneously detected in single-cells
AU2020361681A1 (en) 2019-10-10 2022-05-05 1859, Inc. Methods and systems for microfluidic screening
US20220381781A1 (en) * 2019-10-16 2022-12-01 Georgia Tech Research Corporation Methods for ultrasensitive detection of protein and cellular biomarkers
CN110777048A (zh) * 2019-11-01 2020-02-11 上海市第六人民医院 一种高通量单外泌体分离装置
EP4055160B1 (en) 2019-11-08 2024-04-10 Becton Dickinson and Company Using random priming to obtain full-length v(d)j information for immune repertoire sequencing
WO2021094421A1 (en) * 2019-11-15 2021-05-20 Miltenyi Biotec B.V. & Co. KG Color and bardcoded beads for single cell indexing
US12059679B2 (en) * 2019-11-19 2024-08-13 10X Genomics, Inc. Methods and devices for sorting droplets and particles
US12163189B2 (en) 2019-11-27 2024-12-10 University Of Washington Method for preparation and high-throughput microbial single-cell RNA sequencing of bacteria
GB201918340D0 (en) * 2019-12-12 2020-01-29 Cambridge Entpr Ltd Spatial barcoding
EP4752521A3 (en) 2019-12-23 2026-08-12 10X Genomics, Inc. Reversible fixing reagents and methods of use thereof
EP4081656A1 (en) 2019-12-23 2022-11-02 10X Genomics, Inc. Compositions and methods for using fixed biological samples in partition-based assays
WO2021128035A1 (zh) * 2019-12-25 2021-07-01 苏州绘真生物科技有限公司 高通量单细胞转录组测序方法及试剂盒
CN115279919A (zh) 2020-01-13 2022-11-01 贝克顿迪金森公司 使用含有du的寡核苷酸的细胞捕获
CN115244184A (zh) 2020-01-13 2022-10-25 贝克顿迪金森公司 用于定量蛋白和rna的方法和组合物
US12663345B2 (en) 2020-01-13 2026-06-23 Illumina, Inc. Devices for generating monodisperse droplets from a bulk liquid
JP2023511279A (ja) 2020-01-13 2023-03-17 フルーエント バイオサイエンシーズ インコーポレイテッド 単一細胞シーケンシング
JP7730449B2 (ja) 2020-01-13 2025-08-28 イルミナ インコーポレイテッド 単一細胞遺伝子プロファイリングのための方法およびシステム
WO2021146187A1 (en) 2020-01-13 2021-07-22 Fluent Biosciences Inc. Emulsion based drug screening
EP4090740A4 (en) * 2020-01-14 2024-01-03 President and Fellows of Harvard College Devices and methods for determining nucleic acids using digital droplet pcr and related techniques
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
CN114901831B (zh) * 2020-01-23 2025-03-04 青岛华大智造科技有限责任公司 基于液滴微流控的单细胞测序及应用
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US12416037B2 (en) 2020-01-24 2025-09-16 Vanderbilt University Methods for identification of ligand-blocking antibodies and for determining antibody potency
WO2021155057A1 (en) 2020-01-29 2021-08-05 Becton, Dickinson And Company Barcoded wells for spatial mapping of single cells through sequencing
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
US12449419B1 (en) 2020-02-12 2025-10-21 10X Genomics, Inc. Methods for detecting binding of peptide-MHC monomers to T cells
US12153043B2 (en) 2020-02-25 2024-11-26 Becton, Dickinson And Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
CN111454942A (zh) * 2020-03-16 2020-07-28 张晓鲁 一种同一样品的转录组和基因组同时建立测序文库的构建方法
CA3175931A1 (en) 2020-03-16 2021-09-23 Fluent Biosciences Inc. Multi-omic analysis in monodisperse droplets
JP2023518250A (ja) 2020-03-20 2023-04-28 ミッション バイオ インコーポレイテッド 全ゲノム増幅のためのシングルセルワークフロー
US12428685B2 (en) 2020-03-24 2025-09-30 Illumina, Inc. Viral detection using template emulsification
CN111394432B (zh) * 2020-03-27 2023-03-24 深圳闪量科技有限公司 基于通用探针芯片的多重定量pcr检测系统
EP4143333A1 (en) * 2020-04-30 2023-03-08 Dimensiongen Devices and methods for macromolecular manipulation
GB202007059D0 (en) * 2020-05-13 2020-06-24 Univ Oxford Innovation Ltd Polynucleotide arrays
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
WO2021231779A1 (en) 2020-05-14 2021-11-18 Becton, Dickinson And Company Primers for immune repertoire profiling
EP4153775B1 (en) 2020-05-22 2024-07-24 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
US20230203600A1 (en) * 2020-05-29 2023-06-29 Riken Method for treating cell population and method for analyzing genes included in cell population
CN115803445A (zh) 2020-06-02 2023-03-14 贝克顿迪金森公司 用于5撇基因表达测定的寡核苷酸和珠
US12265079B1 (en) 2020-06-02 2025-04-01 10X Genomics, Inc. Systems and methods for detecting analytes from captured single biological particles
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
CA3189597A1 (en) 2020-07-15 2022-01-20 Fluent Biosciences Inc. Tiered ligation oligos
WO2022026909A1 (en) 2020-07-31 2022-02-03 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
CN114073995B (zh) * 2020-08-13 2026-03-17 安行生物技术有限公司 分析单细胞的微流控设备和单元及其方法
GB202012730D0 (en) * 2020-08-14 2020-09-30 Cambridge Entpr Ltd Modular microfluidic devices, systems and methodds for total RNA analyses
US12655450B1 (en) 2020-10-27 2026-06-16 10X Genomics, Inc. Methods, systems, and kits for detection of nucleic acid molecules
US12480158B1 (en) 2020-11-05 2025-11-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US12084715B1 (en) 2020-11-05 2024-09-10 10X Genomics, Inc. Methods and systems for reducing artifactual antisense products
WO2022099208A1 (en) * 2020-11-09 2022-05-12 The General Hospital Corporation High throughput microfluidics for analysis of immune cell activation
WO2022103712A1 (en) * 2020-11-13 2022-05-19 10X Genomics, Inc. Nano-partitions (encapsulated nucleic acid processing enzymes) for cell-lysis and multiple reactions in partition-based assays
WO2022109343A1 (en) 2020-11-20 2022-05-27 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
US20220243264A1 (en) * 2020-11-23 2022-08-04 The Broad Institute, Inc. Systems and methods for amplifying rna
CN116685850A (zh) 2020-12-15 2023-09-01 贝克顿迪金森公司 单细胞分泌组分析
CN113322254B (zh) * 2021-01-06 2022-05-20 南京诺唯赞生物科技股份有限公司 多靶点蛋白质-dna相互作用的研究方法和工具
US12398262B1 (en) 2021-01-22 2025-08-26 10X Genomics, Inc. Triblock copolymer-based cell stabilization and fixation system and methods of use thereof
CN114836838A (zh) * 2021-02-01 2022-08-02 南方医科大学 一种中通量单细胞拷贝数文库构建的方法及其应用
AU2022227563A1 (en) 2021-02-23 2023-08-24 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
US20250264477A1 (en) 2021-04-14 2025-08-21 Partillion Bioscience Corporation Nanoscale reaction chambers and methods of using the same
EP4330678A1 (en) * 2021-04-26 2024-03-06 Ecole Polytechnique Federale De Lausanne (Epfl) High complexity microcompartment-based interaction screening
WO2022251445A1 (en) 2021-05-28 2022-12-01 Fluent Biosciences Inc. Spatial transcriptomics in pips
US20240376523A1 (en) * 2021-09-14 2024-11-14 Becton, Dickinson And Company Full length single cell rna sequencing
EP4426475A4 (en) 2021-11-03 2025-12-24 Fluent Biosciences Inc DEVICES TO IMPROVE THE PREPARATION AND PROCESSING OF SAMPLES
WO2023154816A1 (en) * 2022-02-09 2023-08-17 Mission Bio, Inc. Systems and methods of detecting merged droplets in single cell sequencing
DK181862B1 (en) * 2022-03-21 2025-02-24 Samplix Aps Method and kit for targeted enrichment of large dna molecules for long-read sequencing using facs or microfluidic partitioning
US12637706B2 (en) 2022-03-23 2026-05-26 10X Genomics, Inc. Methods and compositions related to microsphere surface gelation
WO2023212223A1 (en) * 2022-04-28 2023-11-02 BioSkryb Genomics, Inc. Single cell multiomics
US20240035071A1 (en) 2022-06-17 2024-02-01 10X Genomics, Inc. Catalytic de-crosslinking of samples for in situ analysis
US12534721B2 (en) 2022-10-06 2026-01-27 Illumina, Inc. Quantitative detection and analysis of moleculesip
WO2024097393A1 (en) * 2022-11-04 2024-05-10 Fluid Discovery Systems, compositions, and methods for single cell analysis
US20240253040A1 (en) * 2023-01-30 2024-08-01 Idexx Laboratories, Inc. Methods, Systems, and Device for Electrowetting Bar-Coded Magnetic Beads
WO2025040497A1 (en) * 2023-08-18 2025-02-27 Qiagen Gmbh Method for improving nucleic acid processing
WO2026102372A1 (en) * 2024-11-08 2026-05-15 Arizona Board Of Regents On Behalf Of Arizona State University Methods for in situ genomic dna amplification and barcoding
US20260193640A1 (en) * 2024-12-11 2026-07-09 Nautilus Subsidiary, Inc. Screening and characterization of binding reagents under equilibrium conditions
CN120783873B (zh) * 2025-07-09 2025-12-05 上海锦测医学检验所有限公司 一种智能化蛋白质组学数据异常检测与分析方法

Family Cites Families (122)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0680335A1 (en) 1992-11-30 1995-11-08 The Wellcome Foundation Limited Sequential targeting of tumor sites with oligonucleotide conjugates of antibody and complementary oligonucleotide conjugates of chelated radionuclide
US20020068357A1 (en) 1995-09-28 2002-06-06 Mathies Richard A. Miniaturized integrated nucleic acid processing and analysis device and method
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
US6319507B1 (en) 1997-05-02 2001-11-20 Kobo Products, Inc. Agar gel bead composition and method
ATE358177T1 (de) 1997-07-07 2007-04-15 Medical Res Council Ein in vitro sortierverfahren
US6436677B1 (en) 2000-03-02 2002-08-20 Promega Corporation Method of reverse transcription
AU2002353107A1 (en) 2001-12-11 2003-07-09 Sau Lan Tang Staats Microfluidic devices and methods for two-dimensional separations
JP2006507921A (ja) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体分散のための方法および装置
DE602004026033D1 (de) 2003-01-29 2010-04-29 454 Corp Zweiendige sequenzierung
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
DE10339452A1 (de) 2003-08-22 2005-03-17 Institut für Physikalische Hochtechnologie e.V. Vorrichtung und Verfahren zur Strukturierung von Flüssigkeiten
CN1842368B (zh) 2003-08-27 2014-05-28 哈佛大学 流体物种的电子控制
WO2005030983A2 (en) 2003-09-26 2005-04-07 Circleamp, Inc. Amplification of polynucleotides by rolling circle amplification
EP1691792A4 (en) 2003-11-24 2008-05-28 Yeda Res & Dev COMPOSITIONS AND PROCESSES FOR SORTING IN VITRO OF MOLECULE AND CELL LIBRARIES
KR100647282B1 (ko) 2003-12-24 2006-11-17 삼성전자주식회사 ＤＮＡ 마이크로어레이 스폿팅(Ｓｐｏｔｉｎｇ) 장치 및이를 이용한 스폿팅 방법
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
US20060201868A1 (en) 2005-03-11 2006-09-14 Simmons Blake A Methods and devices for high-throughput dielectrophoretic concentration
US20070068573A1 (en) 2005-08-22 2007-03-29 Applera Corporation Device and method for microfluidic control of a first fluid in contact with a second fluid, wherein the first and second fluids are immiscible
US8383059B2 (en) 2005-09-30 2013-02-26 University Of Utah Research Foundation Microfluidic interface for highly parallel addressing of sensing arrays
US9183349B2 (en) 2005-12-16 2015-11-10 Nextbio Sequence-centric scientific information management
WO2007081385A2 (en) 2006-01-11 2007-07-19 Raindance Technologies, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
CN101432439B (zh) * 2006-02-24 2013-07-24 考利达基因组股份有限公司 Dna阵列上的高通量基因组测序
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
WO2007133710A2 (en) 2006-05-11 2007-11-22 Raindance Technologies, Inc. Microfluidic devices and methods of use thereof
WO2007140015A2 (en) * 2006-05-26 2007-12-06 Althea Technologies, Inc Biochemical analysis of partitioned cells
AU2007255110B8 (en) 2006-06-02 2014-04-10 Pfizer Products Inc. Circulating tumor cell assay
CA2669746C (en) 2006-12-14 2017-05-16 Dow Agrosciences Llc Optimized non-canonical zinc finger proteins
US8338166B2 (en) 2007-01-04 2012-12-25 Lawrence Livermore National Security, Llc Sorting, amplification, detection, and identification of nucleic acid subsequences in a complex mixture
WO2008109176A2 (en) 2007-03-07 2008-09-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
WO2009015296A1 (en) 2007-07-24 2009-01-29 The Regents Of The University Of California Microfabricated dropley generator
US8268564B2 (en) 2007-09-26 2012-09-18 President And Fellows Of Harvard College Methods and applications for stitched DNA barcodes
GB0720202D0 (en) * 2007-10-16 2007-11-28 Cambridge Entpr Ltd Microfluidic systems
WO2009092106A1 (en) 2008-01-18 2009-07-23 The Regents Of The University Of California Accurate and rapid micromixer for integrated microfluidic devices
JP5737707B2 (ja) 2008-03-04 2015-06-17 クリスタル バイオサイエンス インク．Ｃｒｙｓｔａｌ Ｂｉｏｓｃｉｅｎｃｅ Ｉｎｃ． ゲル状マイクロドロップ組成物およびその使用方法
US9409177B2 (en) 2008-03-21 2016-08-09 Lawrence Livermore National Security, Llc Chip-based device for parallel sorting, amplification, detection, and identification of nucleic acid subsequences
US8852952B2 (en) 2008-05-03 2014-10-07 Advanced Liquid Logic, Inc. Method of loading a droplet actuator
WO2011120020A1 (en) 2010-03-25 2011-09-29 Quantalife, Inc. Droplet transport system for detection
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US20140179544A1 (en) 2012-09-07 2014-06-26 Bio-Rad Laboratories, Inc. Compositions, systems and methods for droplet formation, spacing and detection
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US20130084572A1 (en) 2011-09-30 2013-04-04 Quantalife, Inc. Calibrations and controls for droplet-based assays
US8709762B2 (en) 2010-03-02 2014-04-29 Bio-Rad Laboratories, Inc. System for hot-start amplification via a multiple emulsion
JP5457222B2 (ja) 2009-02-25 2014-04-02 エフ．ホフマン−ラ ロシュ アーゲー 小型化ハイスループット核酸分析
CN102459592B (zh) 2009-06-15 2017-04-05 考利达基因组股份有限公司 用于长片段阅读测序的方法和组合物
CA2766795C (en) 2009-06-26 2017-10-03 President And Fellows Of Harvard College Fluid injection
CA2767056C (en) 2009-09-02 2018-12-04 Bio-Rad Laboratories, Inc. System for mixing fluids by coalescence of multiple emulsions
US8746285B2 (en) 2009-09-04 2014-06-10 Auburn University Programmable fluidic droplet generation
US9625454B2 (en) * 2009-09-04 2017-04-18 The Research Foundation For The State University Of New York Rapid and continuous analyte processing in droplet microfluidic devices
US9376713B2 (en) 2009-09-23 2016-06-28 The Board Of Trustees Of The University Of Illinois Label free detection of nucleic acid amplification
US9890408B2 (en) 2009-10-15 2018-02-13 Ibis Biosciences, Inc. Multiple displacement amplification
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US20120270739A1 (en) 2010-01-19 2012-10-25 Verinata Health, Inc. Method for sample analysis of aneuploidies in maternal samples
AU2013203624B2 (en) 2010-02-09 2015-06-25 General Electric Company Isothermal amplification of nucleic acid using a mixture of randomized primers and specific primers
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
JP2013524171A (ja) 2010-03-25 2013-06-17 クァンタライフ・インコーポレーテッド 液滴ベースのアッセイのための液滴の発生
JP5573335B2 (ja) 2010-04-28 2014-08-20 株式会社島津製作所 磁性体粒子操作デバイス及び磁性体粒子操作方法
ES2539047T3 (es) 2010-06-10 2015-06-25 Albert-Ludwigs-Universität Freiburg Aparato y método para dispensar células o partículas confinadas en una gota en vuelo libre
US9650629B2 (en) 2010-07-07 2017-05-16 Roche Molecular Systems, Inc. Clonal pre-amplification in emulsion
CA2808118C (en) 2010-07-22 2016-04-19 Kieran Curran Composite liquid cells
US9695390B2 (en) 2010-08-23 2017-07-04 President And Fellows Of Harvard College Acoustic waves in microfluidics
WO2012045012A2 (en) 2010-09-30 2012-04-05 Raindance Technologies, Inc. Sandwich assays in droplets
EP2436444A1 (en) 2010-10-01 2012-04-04 Centre National de la Recherche Scientifique (C.N.R.S.) Microfluidic device for production and collection of droplets of a fluid
US9902950B2 (en) 2010-10-08 2018-02-27 President And Fellows Of Harvard College High-throughput single cell barcoding
US20120258870A1 (en) 2010-11-22 2012-10-11 The University Of Chicago Methods, Systems, and/or Use of Oligonucleotide Conjugates to Develop Panels for Use in Assays and Detections
WO2012083225A2 (en) * 2010-12-16 2012-06-21 Gigagen, Inc. System and methods for massively parallel analysis of nycleic acids in single cells
US20130295587A1 (en) 2011-01-24 2013-11-07 Ge Healthcare Bio-Sciences Ab Method of coupling binding agents to a substrate surface
US8765455B2 (en) 2011-01-27 2014-07-01 Lawrence Livermore National Security, Llc Chip-based droplet sorting
CN103703143B (zh) * 2011-01-31 2016-12-14 爱普瑞斯生物公司 鉴定细胞中的多个表位的方法
JP6017458B2 (ja) 2011-02-02 2016-11-02 ユニヴァーシティ・オブ・ワシントン・スルー・イッツ・センター・フォー・コマーシャリゼーション 大量並列連続性マッピング
EP3859011A1 (en) * 2011-02-11 2021-08-04 Bio-Rad Laboratories, Inc. Methods for forming mixed droplets
WO2012112804A1 (en) * 2011-02-18 2012-08-23 Raindance Technoligies, Inc. Compositions and methods for molecular labeling
EP3246416B1 (en) 2011-04-15 2024-06-05 The Johns Hopkins University Safe sequencing system
JP2014512826A (ja) 2011-04-25 2014-05-29 バイオ−ラド ラボラトリーズ インコーポレイテッド 核酸分析のための方法および組成物
SG10201605049QA (en) 2011-05-20 2016-07-28 Fluidigm Corp Nucleic acid encoding reactions
BR112013029729A2 (pt) 2011-05-23 2017-01-24 Basf Se controle de emulsões, incluindo emulsões múltiplas
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
EP2714970B1 (en) 2011-06-02 2017-04-19 Raindance Technologies, Inc. Enzyme quantification
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
US20130189700A1 (en) 2011-07-25 2013-07-25 Bio-Rad Laboratories, Inc. Breakage of an emulsion containing nucleic acid
GB2485635B (en) 2011-07-26 2012-11-28 Verinata Health Inc Method for determining the presence or absence of different aneuploidies in a sample
US20130032235A1 (en) 2011-08-02 2013-02-07 Teledyne Dalsa Semiconductor, Inc. Integrated microfluidic check valve and device including such a check valve
US9469874B2 (en) * 2011-10-18 2016-10-18 The Regents Of The University Of California Long-range barcode labeling-sequencing
WO2013095469A1 (en) 2011-12-21 2013-06-27 Intel Corporation Psmi using at-speed scan capture
WO2013119753A1 (en) 2012-02-08 2013-08-15 President And Fellows Of Harvard College Droplet formation using fluid breakup
US9176031B2 (en) 2012-02-24 2015-11-03 Raindance Technologies, Inc. Labeling and sample preparation for sequencing
WO2013130512A2 (en) 2012-02-27 2013-09-06 The University Of North Carolina At Chapel Hill Methods and uses for molecular tags
EP2823064B1 (en) 2012-03-05 2019-02-06 President and Fellows of Harvard College Methods for epigenetic sequencing
WO2013173394A2 (en) * 2012-05-14 2013-11-21 Cb Biotechnologies, Inc. Method for increasing accuracy in quantitative detection of polynucleotides
WO2014028378A2 (en) 2012-08-13 2014-02-20 The Regents Of The University Of California Methods and systems for detecting biological components
CN114891871A (zh) 2012-08-14 2022-08-12 10X基因组学有限公司 微胶囊组合物及方法
US20140378349A1 (en) * 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
US20140378345A1 (en) * 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
WO2014047556A1 (en) 2012-09-21 2014-03-27 The Broad Institute, Inc. Compositions and methods for long insert, paired end libraries of nucleic acids in emulsion droplets
CN104812492A (zh) 2012-11-27 2015-07-29 基因细胞生物系统有限公司 处理液体样品
EP3567116A1 (en) 2012-12-14 2019-11-13 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014108323A1 (en) 2013-01-10 2014-07-17 Eth Zurich Method and apparatus for depositing droplets onto a substrate
CN105324489A (zh) 2013-03-04 2016-02-10 韦拉克斯生物医学股份有限公司 多分析物试验
WO2014153071A1 (en) 2013-03-14 2014-09-25 The Broad Institute, Inc. Methods for quantitating dna using digital multiple displacement amplification
US9752179B2 (en) * 2013-03-14 2017-09-05 Cold Spring Harbor Laboratory Trans-splicing transcriptome profiling
WO2014144495A1 (en) * 2013-03-15 2014-09-18 Abvitro, Inc. Single cell bar-coding for antibody discovery
AU2014233699B2 (en) 2013-03-15 2018-05-24 The Regents Of The University Of California High-speed on demand microfluidic droplet generation and manipulation
US9328382B2 (en) * 2013-03-15 2016-05-03 Complete Genomics, Inc. Multiple tagging of individual long DNA fragments
DE102013207900A1 (de) 2013-04-30 2014-10-30 Volkswagen Ag Membran-Elektroden-Einheit und Brennstoffzelle mit einer solchen
CN105705659B (zh) 2013-08-28 2019-11-29 贝克顿迪金森公司 大规模平行单细胞分析
US10947587B2 (en) 2013-11-05 2021-03-16 The Regents Of The University Of California Single-cell forensic short tandem repeat typing within microfluidic droplets
EP3105326A4 (en) 2014-02-10 2018-01-10 Gencell Biosystems Limited Composite liquid cell (clc) mediated nucleic acid library preparation device, and methods for using the same
CN113215221A (zh) 2014-04-08 2021-08-06 华盛顿大学商业中心 用于使用多分散小滴执行数字检定的方法和设备
EP3134536B1 (en) 2014-04-21 2019-12-18 President and Fellows of Harvard College Systems and methods for barcoding nucleic acids
CN110333347A (zh) 2014-05-23 2019-10-15 萤火虫生物股份有限公司 用于生物测定的底物介导的反应器
WO2015189336A1 (en) 2014-06-11 2015-12-17 Samplix S.A.R.L. Nucleotide sequence exclusion enrichment by droplet sorting (needls)
EP4574974A3 (en) * 2014-06-26 2025-10-08 10x Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
EP3160654A4 (en) 2014-06-27 2017-11-15 The Regents of The University of California Pcr-activated sorting (pas)
WO2016064755A2 (en) 2014-10-20 2016-04-28 The Regents Of The University Of Califronia Rapid modulation of droplet composition with membrane microvalves
US10434507B2 (en) 2014-10-22 2019-10-08 The Regents Of The University Of California High definition microdroplet printer
CN107530654A (zh) 2015-02-04 2018-01-02 加利福尼亚大学董事会 通过在离散实体中条形码化对核酸进行测序
CA2974299A1 (en) 2015-02-04 2016-08-11 The Regents Of The University Of California Multiple-emulsion nucleic acid amplification
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US20170009274A1 (en) 2017-01-12
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US12448643B2 (en) 2025-10-21

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