本申请是申请日为2013年1月29日的、发明名称为“从经基因改造的微生物生产黏康酸”的中国专利申请201380017560.1(PCT/US2013/023690)的分案申请。
实施例17
通过发酵生产顺,顺-黏康酸
可以通过以上实施例1-15中所公开的经基因改造的微生物来生产顺,顺-黏康酸。生长培养基可以广泛地不同,并且可以是任何支持微生物充分生长的培养基。优选的培养基是含有矿物盐和非芳族碳源例如葡萄糖、木糖、乳糖、甘油、乙酸、阿拉伯糖、半乳糖、甘露糖、麦芽糖或蔗糖的基本培养基(关于优选基本生长培养基的实例参见上文)。对于经改造的微生物和生长培养基的各个组合,通过常规实验来确定生产顺,顺-黏康酸的合适条件,在所述常规实验中,发酵参数系统性地变化,例如温度、pH、通气速率和用于维持pH的化合物。随着顺,顺-黏康酸产生,必须向发酵罐中补料一种或多种化合物以防止pH变得太低。用来中和酸的优选化合物包括碱性盐,如铵、钠、钾、钙、镁的氧化物、氢氧化物、碳酸盐和碳酸氢盐,或者两种或更多种的这类碱盐的组合。
大肠杆菌MYR428菌株在7升发酵罐中的黏康酸生产示于图18。对具有基因型ΔaroEΔackA::P15-aroBΔpoxB::tktA的大肠杆菌MYR261菌株转化质粒pCP32AMP和pMG37以产生MYR428。MYR428在如上所述的7升发酵罐中以葡萄糖补料生长48小时。最终黏康酸滴度为16g/l(参见图18)。
在发酵完成后,通过絮凝、离心和/或过滤来移除细胞,然后通过一个或多个后续步骤例如沉淀、结晶、电渗析、层析(离子交换型、疏水亲和性、和/或基于尺寸的)、微滤、纳滤、反渗透和蒸发的组合从澄清发酵液中纯化顺,顺-黏康酸。
本发明公开了如下实施方案:
1.从非芳族碳源开始生产顺,顺-黏康酸的经基因改造的微生物,其中将所有编码在黏康酸通路中起作用的蛋白质的基因整合到所述微生物的染色体DNA中。
2.实施方案1的经基因改造的微生物,其中所述编码在黏康酸通路中起作用的蛋白质的基因选自由aroZ、qa-4、asbF、quiC、aroY、和catAX组成的组。
3.实施方案1的经基因改造的微生物生物体,其包含编码不动杆菌属(Acinetobacter)细菌的QuiC酶或所述QuiC酶的同源物的基因。
4.实施方案1的经基因改造的微生物,其包含一种或多种编码在莽草酸通路中起作用的蛋白质的外源基因。
5.实施方案4的经基因改造的微生物,其中将所述编码在莽草酸通路中起作用的蛋白质的外源基因中的一种或多种整合到所述生物体的染色体DNA中。
6.实施方案4的经基因改造的微生物,其中所述编码在莽草酸通路中起作用的蛋白质的外源基因选自由aroB、aroD、aroF、aroG、aroH、tktA、talB、rpe和rpi组成的组。
7.实施方案4的经基因改造的生物体,其中所述编码在黏康酸通路或莽草酸通路中起作用的蛋白质的基因无一从需要补料化学品用于诱导的启动子表达。
8.实施方案1的经基因改造的宿主细菌,其中芳族氨基酸生物合成的负调节蛋白的活性实质上降低或消除。
9.实施方案8的经基因改造的微生物,其中芳族氨基酸生物合成的负调节蛋白是TyrR蛋白或TyrR蛋白的同源物。
10.实施方案8的经基因改造的微生物,其中编码芳族氨基酸生物合成的负调节蛋白的基因已被突变。
11.实施方案1的经基因改造的生物体,其包含编码渗漏AroE酶的aroE基因。
12.实施方案1的经基因改造的微生物生物体,其中至少一种在莽草酸通路中有活性的酶实质上抵抗所述微生物的代谢物的抑制。
13.实施方案12的经基因改造的生物体,其包含aroG*基因,所述aroG*基因编码实质上抵抗苯丙氨酸抑制的DAHP合酶。
14.实施方案12的经基因改造的生物体,其包含编码DAHP合酶的aroG*基因,所述aroG*基因选自由aroG*20-893、aroG*20-897、aroG*20-899、aroG*20-901、aroG*111、aroG*211、aroG*212、aroG*311、aroG*312、aroG*411、aroG*412和aroG*511组成的组。
15.实施方案1的经基因改造的微生物,其中所述微生物是细菌。
16.实施方案1的经基因改造的微生物,其中所述微生物衍生自大肠杆菌(Escherichia coli)C。
17.经基因改造的细菌,其在PEP依赖性磷酸转移酶体系中有缺陷、在用于葡萄糖输入的基于GalP蛋白的体系中有缺陷,且包含编码功能性的葡萄糖易化扩散蛋白的外源基因。
18.如实施方案17中的经基因改造的细菌,其中功能性的葡萄糖易化扩散蛋白是由glf基因编码的Glf蛋白。
19.如实施方案17中的经基因改造的细菌,还包含编码葡糖激酶的外源基因。
20.如实施方案17中的经基因改造的细菌,还包含一种或多种外源基因,所述外源基因编码在从非芳族碳源开始生产顺,顺-黏康酸的黏康酸通路中起作用的蛋白质。
21.实施方案20的经基因改造的细菌,其中所述编码在黏康酸通路中起作用的蛋白质的基因选自由aroZ、qa-4、asbF、quiC、aroY和catAX组成的组。
22.如实施方案17中的经基因改造的细菌,还包含一种或多种在染色体上整合的外源基因,所述外源基因编码在莽草酸通路中起作用的蛋白质。
23.如实施方案22中的经基因改造的细菌,其中所述编码在莽草酸通路中起作用的蛋白质的外源基因选自由aroB、aroD、aroF、aroG、aroH、tktA、talB、rpe和rpi组成的组。
24.实施方案17的经基因改造的细菌,其中芳族氨基酸生物合成的负调节蛋白的活性被实质上降低或消除。
25.实施方案17的经基因改造的细菌,其中芳族氨基酸生物合成的负调节蛋白是TyrR蛋白或TyrR蛋白的同源物。
26.实施方案17的经基因改造的细菌,其中编码芳族氨基酸生物合成的负调节蛋白的基因已被缺失或突变。
27.实施方案17的经基因改造的细菌,其中至少一种在莽草酸通路中有活性的酶实质上抵抗所述微生物的代谢物的抑制。
28.实施方案17的经基因改造的细菌,其中所述微生物衍生自大肠杆菌C。
29.从非芳族碳源开始生产顺,顺-黏康酸的经基因改造的微生物,其中编码在黏康酸通路和莽草酸通路中起作用的蛋白质的外源基因不从需要补料化学品用于诱导的启动子表达。
30.如实施方案29中的经基因改造的微生物,其中所述微生物是细菌。
31.如实施方案29中的经基因改造的微生物,其中所述微生物是大肠杆菌。
32.从非芳族碳源开始产生顺,顺-黏康酸的经基因改造的微生物,所述微生物包含编码渗漏AroE酶的aroE基因。
33.如实施方案32中的经基因改造的微生物,其中所述微生物是细菌。
34.如实施方案32中的经基因改造的微生物,其中所述微生物是大肠杆菌。
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Stroman,P.,Reinert,W.R.,and Giles,N.H.(1978)Purification andcharacterization of3-dehydroshikimate dehydratase,an enzyme in the induciblequinic acid catabolic pathway of Neurospora crassa,J Biol Chem 253,4593-4598.
Tang,J.,Zhu,X.,Lu,J.and Liu,P.(2012)Recruiting alternative glucoseutilization pathways for improving succinate production.App MicrobiolBiotechnol DOI 10,1007/s00253-012-434.1
Tateoka,T.,and Yasuda,I.(1995)3-Dehydroshikimate dehydratase in munghean cultured cells,Plant Cell Reports 15,212-217.
Weaver,L.M.,and Herrmann,K.M.(1990)Cloning of an aroF allele encodinga tyrosine-insensitive 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase,JBacteriol 172,6581-6584.
Weber,C.,Bruckner,C.,Weinreb,S.,Lehr,C.,Essl,C.and Bole,E.(2012)Biosynthesis of cis,cis-muconic acid and its aromatic precursors catechol andproteocatechuic acid,from renewable feedstocks by Saccharomyces cerevisiae,App Environ Microbiol.78,8421-8430.
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序列表
<110> 麦兰特公司
<120> 从经基因改造的微生物生产黏康酸
<130> MC2013-01PCT
<150> US 61/632,777
<151> 2012-01-30
<160> 41
<170> PatentIn version 3.5
<210> 1
<211> 195
<212> DNA
<213> P15 启动子;
<400> 1
gctattgacg acagctatgg ttcactgtcc accaaccaaa actgtgctca gtaccgccaa 60
tatttctccc ttgaggggta caaagaggtg tccctagaag agatccacgc tgtgtaaaaa 120
ttttacaaaa aggtattgac tttccctaca gggtgtgtaa taatttaatt acaggcgggg 180
gcaaccccgc ctgtt 195
<210> 2
<211> 164
<212> DNA
<213> P26 启动子;
<400> 2
gcctacctag cttccaagaa agatatccta acagcacaag agcggaaaga tgttttgttc 60
tacatccaga acaacctctg ctaaaattcc tgaaaaattt tgcaaaaagt tgttgacttt 120
atctacaagg tgtggtataa taatcttaac aacagcagga cgct 164
<210> 3
<211> 91
<212> DNA
<213> PR 启动子;
<400> 3
acgttaaatc tatcaccgca agggataaat atctaacacc gtgcgtgttg actattttac 60
ctctggcggt gataatggtt gcatgtacaa g 91
<210> 4
<211> 359
<212> PRT
<213> 由qa-4基因编码的3-脱氢莽草酸脱水酶;
<400> 4
Met Pro Ser Lys Leu Ala Ile Ser Ser Met Ser Leu Gly Arg Cys Phe
1 5 10 15
Ala Gly His Ser Leu Asp Ser Lys Leu Asp Ala Ala Gln Arg Tyr Gly
20 25 30
Tyr Leu Gly Ile Glu Leu Phe Tyr Glu Asp Leu Val Asp Val Ala Glu
35 40 45
His Leu Ser Asn Glu Arg Pro Ser Pro Glu Gly Pro Phe Val Glu Ala
50 55 60
Gln Ile Ala Ala Ala Arg His Ile Leu Gln Met Cys Gln Ala Arg Gly
65 70 75 80
Leu Glu Val Val Cys Leu Gln Pro Phe Met His Tyr Asp Gly Leu Asn
85 90 95
Asp Arg Ala Glu His Glu Arg Arg Leu Glu Lys Leu Ala Leu Trp Ile
100 105 110
Glu Leu Ala His Glu Leu His Thr Asp Ile Ile Gln Ile Pro Ala Asn
115 120 125
Phe Leu Pro Ala Asn Gln Val Ser Asp Asn Leu Asp Leu Ile Val Ser
130 135 140
Asp Leu Cys Lys Val Ala Asp Ile Gly Ala Gln Ala Leu Pro Pro Ile
145 150 155 160
Arg Phe Ala Tyr Glu Ser Leu Cys Trp Ser Thr Arg Val Asp Leu Trp
165 170 175
Glu Arg Cys Trp Asp Ile Val Gln Arg Val Asp Arg Pro Asn Phe Gly
180 185 190
Ile Cys Leu Asp Thr Phe Asn Ile Leu Gly Arg Ile Tyr Ala Asp Pro
195 200 205
Thr Ser Pro Ser Gly Arg Thr Pro Asn Ala Lys Glu Ala Val Arg Lys
210 215 220
Ser Ile Ala Asn Leu Val Ser Arg Val Asp Val Ser Lys Val Phe Tyr
225 230 235 240
Val Gln Val Val Asp Ala Glu Arg Leu Ser Lys Pro Leu Leu Pro Gly
245 250 255
His Pro Tyr Tyr Asn Pro Glu Gln Pro Ala Arg Met Ser Trp Ser Arg
260 265 270
Asn Cys Arg Leu Phe Tyr Gly Glu Thr Glu Tyr Gly Ala Tyr Leu Pro
275 280 285
Val Lys Glu Val Ala Arg Ala Leu Phe His Gly Ile Gly Phe Glu Gly
290 295 300
Trp Val Ser Leu Glu Leu Phe Asn Arg Arg Met Ser Glu Glu Gly Pro
305 310 315 320
Glu Val Pro Glu Glu Leu Ala Met Arg Gly Ala Ile Ser Trp Ala Lys
325 330 335
Leu Val Gln Asp Leu Arg Ile Pro Val Glu Gly Pro Leu Val Thr Met
340 345 350
Pro Arg Val Ser Ala Ser Leu
355
<210> 5
<211> 2160
<212> DNA
<213> 来自粗糙脉孢菌的qa-4基因加周围序列;
<400> 5
gaattcggga aatggaatct tacctgggaa ccgaaatcac agtccgggta ggttatagag 60
catatagtga actgtcaaag ttctagacct ggaccagcca cttggagtcg ttgttttagt 120
tatacctaca ttcactcact gttgactttc aatcatactt acttagacgg agcaacgcgc 180
cagaatccaa attgttgcat agttgcggta tcaccaagtg gcttcccata atagtttgcc 240
attcgatgag acagctaact ggaagaccgg tactcgcagg ttgcacgatt acacggaagg 300
attcggtatt ccgtgtttca tctgtcaaag tccctttcca tatgaatccg aggtactatg 360
actggatctc gatacaagct ggccagcgag gtgcctgcct tgacaggctg tcaactgcgg 420
gacggccggc taagtgttta acacgcaagg gtggaagatg tctcgtcccg tcatccaaga 480
ccgtcaacat tcgaggccat ctgatcgttg aagagatgct aaatcttgtg aaacgctcat 540
aggtcgctta ccttcggccc acccgttaat gctttattcc gctgagcaaa cttcggcttc 600
catcccgcgg ttcaccgttt acatcactta tcgttgcggt tattggccga ttcttcgcaa 660
accgaaacga tgacatcccg aatatctgca atacaccgcc acggccggcg tctttatcac 720
acctcctatg ggagacgaaa gtgccttgat acccctagtc atttgaagat tcaggatggg 780
agacggctgg ccgcttgcgg agttacgttc gagtcttggt cgcaggaacg cttgccgtat 840
tgaatgagac cccgagaagg tcaaatcaaa tcttggaaga ccccaactgc ttcctcattg 900
ccttcactcc ccatatcaat ggggcacatc ctgtgactac cttggtgctt tatttcctca 960
ccatttggcg atacaagctc aaggacaccg aggtgatata cagttcttca aggacactat 1020
ctcacctcaa tatcaagaac cagtctcatc atctcttatt tctccaggat ccccccacca 1080
acaacatcgg cttttttttt tcccctattc tcaagaccca tcaagacgct cacttcgctg 1140
agcctttcgc catgccgtca aagctagcca ttagttccat gtccctaggg cgctgctttg 1200
ccggccactc tctggacagc aagcttgatg ccgctcaacg atacggctat cttggtatcg 1260
agctttttta tgaggatctg gtcgacgttg cagagcattt gtcgaacgag cgtccctctc 1320
ccgaaggccc ttttgtcgaa gctcagatag ccgccgctcg tcatattctc cagatgtgtc 1380
aagccagggg gcttgaggtc gtctgcctcc agcctttcat gcactacgac ggccttaacg 1440
acagggcaga acatgagcgt cgtctggaga agctagcact atggattgag ctcgctcatg 1500
agcttcacac cgacatcatt cagatcccag ccaacttcct ccctgccaac caagtcagtg 1560
acaacctcga cctgattgtc tcagatcttt gcaaggtggc cgatattgga gctcaagctt 1620
tgccccctat ccgctttgcc tacgagagtc tttgctggag cacccgtgtc gacctctggg 1680
agcgctgctg ggacatcgta caacgcgttg accgccccaa ctttggcatt tgccttgaca 1740
ccttcaacat cctcggccgc atctatgccg accctacatc tcctagcggt aggacaccca 1800
acgcaaaaga ggcagtcagg aagtccatcg ccaacttggt ctcgcgcgtg gatgtctcca 1860
aagtcttcta cgtccaggtg gttgacgccg agaggctgag caagccacta ctgcccggtc 1920
acccgtatta caatccagag cagccggcga ggatgagctg gtcgcgcaat tgtagactgt 1980
tctacggcga aacagaatat ggtgcgtatc ttcccgtgaa ggaggttgct cgagcccttt 2040
tccacggcat tggtttcgag ggctgggtca gtttggagct tttcaaccgc agaatgtctg 2100
aggagggacc tgaagtgccg gaggaacttg ccatgagagg cgctatctcg tgggccaagt 2160
<210> 6
<211> 348
<212> PRT
<213> 由qutC基因编码的来自构巢曲霉的QutC;
<400> 6
Met Pro Ala Asn Leu Lys Ile Gly Ile Pro Thr Val Ser Leu Ser Lys
1 5 10 15
Pro Gly Leu His Ser Leu Asp His Lys Leu Arg Ser Ala Ala His Gly
20 25 30
Phe Ala Gly Ile Glu Leu Phe Ile Asp Asp Leu Ser His Phe Ala Ser
35 40 45
Ser Ser Phe Asn Gly Ser Leu Thr Gln Ala Ala Lys Tyr Ile Ser Ser
50 55 60
Leu Ala Lys Gln Leu Asn Leu Thr Phe Ile Cys Leu Gln Pro Phe Gly
65 70 75 80
Phe Tyr Glu Gly Leu Val Asp Thr Asn Gln Ser Thr Tyr Leu Leu Thr
85 90 95
Glu Lys Leu Pro Leu Trp Phe Ala Ile Ala Arg Ile Ile Gly Thr Asp
100 105 110
Leu Ile Gln Ile Pro Ala Asn Phe Leu Gln Asn Asp Pro Val Thr Gly
115 120 125
Ala Ala Arg Thr Ser Gly Asp Ile Arg Leu Ile Val Ser Asp Leu Gln
130 135 140
Thr Ile Ala Asp Ile Gly Val Lys Gln Gly Phe Arg Phe Val Tyr Glu
145 150 155 160
Ala Leu Cys Trp Ser Thr His Val Asp Thr Trp Glu Ala Ala Trp Asn
165 170 175
Val Val Lys Leu Val Asp Arg Glu Asn Phe Gly Ile Cys Leu Asp Ser
180 185 190
Phe Asn Thr Arg Thr Pro Leu Pro Ser Leu Gly Arg Arg Arg Met Leu
195 200 205
Ser Lys Pro Trp Pro Ser Pro Trp Arg Arg Ser Val Leu Ser Ser Pro
210 215 220
Val Glu Asn Trp Thr Ser Gly Lys Ser Ser Thr Ser Ser Leu Ser Met
225 230 235 240
Ala Ser Gly Cys Arg Arg Arg Trp Thr Arg Ser Thr Pro Phe Met Trp
245 250 255
Arg Ala Asn Pro Arg Arg Met Ser Trp Ser Arg Asn Ala Arg Leu Phe
260 265 270
Pro Cys Glu Glu Glu Arg Gly Gly Tyr Leu Pro Val Leu Glu Ile Ala
275 280 285
Arg Ala Phe Phe Glu Ile Gly Phe Glu Gly Trp Val Ser Leu Glu Leu
290 295 300
Phe Ser Arg Thr Cys Asn Asp Pro Asp Val Asn Thr Val Gly Glu His
305 310 315 320
Ala Arg Arg Gly Met Asp Arg Arg Arg Arg Val Val Ala Ala Leu Gly
325 330 335
Leu Asp Val Glu Val Pro Ala Arg Asn Cys Glu Cys
340 345
<210> 7
<211> 3298
<212> DNA
<213> 来自构巢曲霉的qutC基因的加周围序列;
<400> 7
aagcttggtt tcaagtgatg atatatagtt atgaggatat aatatgaacc gaaagacgat 60
gtttcttgtg aatatttacg tgatagttgt ctgtctaata tggtacagca gtagaacaac 120
tacatacggt cactacttac agccctagtc attccctccc tcgattgcct accatttata 180
cactttgaac atccacaggc ttgcctccct ccatactctc cctaacagct tgaacaactc 240
tgagcgccct caccccatct tcaacgccac agccaactcc tcgctcgcca tcctcaccct 300
ttccactaac aacatcaaca aaatatcccc actgcgcatc aaacggcctc acatcagcat 360
ccttcactga aatctgctgc atcgccaact ccgtattcca gcctttctct ttcccctgtc 420
cacaagaaac atggtcatag ctccagcgcg tcatatcagg cacactgaga ctcgctctgg 480
ttccaagaat tcgataacag tcgcttgcac tcttggaagg agcgggcgga atcgtaggat 540
tctcgcccgt tcctgtttca aagttcaacg gcgaaggcgt cgcgtcgcag atgagaaatg 600
tgcctactat cccagacgca aagcgcagcg tcacagcaca gccttcctcg gcggtatgtt 660
ccgggttctg gcgcatgcgc tgcaggagtg taccctccgc gtagacccta ctgacgggcc 720
caaacagaaa ctgcagcacg tcgatatcgt ggataagatt aatccccagc acgccgccct 780
tcttcttatc tgcgcgccaa gaaccgagcg gcggcgcgaa gtaagaggcc ggcttcagaa 840
gtgtccagag gccgttcact gcaacgacgg tgccgagtga gtctgtctct aacaaagact 900
ttgtggtttg gatgtacgga ttgaagcggc ggtggtggcc gatctggatg ttgatcttcg 960
catccttgcc tctcttctca tctttacatt tctgttcctt gacggtagcg aggaggtgct 1020
cggccgactc cagatcgtca ctgatcggtt tctcaaggag gatattgcgg attccgttct 1080
ccagcagctg gagcgtgacg tccacgtgcg tgtgattggg cgtgctcacg atcgccgcgt 1140
ctggtttccc ggttgtctta ccgacaacgt ctaacataga cgtaatagaa tcatagcaag 1200
gaacgccaaa tgattctgcg accgggattg cagagggtga agggtcaaca aaagcgatca 1260
gctgggttcg tgggtgtcgt tgcacggatt gtgcgtgacg gggcccaata agtccggcac 1320
cgacgatgac aatgaggata ttcttgtcct tttccttgct gcagggcacc attgtgcatg 1380
tcggtggctg gaaataaaca gaacagggat atggtcaagt cggagaatcg gtgcaggata 1440
gaccggctac ttgatgtagg acgacagtcg cgatctaccg agagcgtgag attcactgtg 1500
ggactgattt atgtaatttg aggcgcagca gacttaggga cttgaaatgt ggctgtctgt 1560
ggatgcattt gcggggtatg gagtacagag tgcatacagc tgtgtatatg gagttcctta 1620
cggagagggt gacctggtat ggggagaacg ggcaaaatgc tcacccggca acctctcaaa 1680
gcgtttaccc ggtatactcc tctgatatca atatttccaa tcagcaccta tatcatcacg 1740
acgctctcct gaggattccg tagctaaccg ccctggatcc tacattaata aataagccat 1800
ttgctttttc tgctgcgagt gtgattctca atacgattac gtatcacatg cagattgcct 1860
ttacttcagc tgcatttgat cagccacagc tctaagagca aacataccct acctacctac 1920
ctacttcgcc tagggtacat aatcaccgcc atctcctcct cgatcagtct tcaactcaat 1980
cagctcattc attctattct taatataata tataccttta gatctccagc agagacccga 2040
agagtcggca attcaaaatg cccgcaaacc tcaaaatcgg tatcccaacc gtgtccctgt 2100
caaaaccggg cctgcactct cttgaccata agctccgctc ggccgctcat ggcttcgcgg 2160
ggatcgagct gtttattgat gacctctccc atttcgcctc atcgtcattc aatggctccc 2220
tcactcaagc ggcaaagtat atctcctcgc tcgccaagca acttaacctc acatttatct 2280
gcctgcaacc attcggtttc tacgagggtc tggtggacac aaatcagtcg acgtacctgc 2340
tcactgagaa actcccgctc tggtttgcga tcgcccgcat tataggcaca gatctcatcc 2400
aaatccccgc aaatttcctc cagaatgacc ctgtcaccgg ggctgcacga acaagcggcg 2460
acataaggct tatcgtctca gatctgcaga cgatcgcaga tatcggtgta aagcagggct 2520
tccgctttgt gtacgaggcg ctctgctggt cgacgcatgt cgatacatgg gaagcagcgt 2580
ggaatgtcgt caagctggtt gatagagaga atttcgggat ctgcctggat agcttcaaca 2640
cgcggacccc gcttccgtca ctgggaagac gccggatgct gagcaagccg tggccaagtc 2700
catggagacg ctccgttctc tcgtctccag tggagaactg gacatcagga aaatcttcta 2760
catccagctt gtcgatggcg agcggttgtc ggcgccgctg gacgagaagc acccctttca 2820
tgtggagggc caacccccga agaatgagct ggagtcgcaa tgcgcggtta ttcccctgtg 2880
aagaggagag gggtgggtat cttcctgtgt tggagatcgc gagggcgttc tttgaaatcg 2940
ggttcgaggg gtgggtgagt ctagagctgt tttcaaggac gtgtaatgat cccgatgtga 3000
acacggtggg ggagcatgcg agacgtggga tggatagaag gaggagggtt gttgcggcgc 3060
taggactcga tgttgaggtg ccagcacgta actgtgaatg ttagcatgaa cggcaaggag 3120
agggtggagg tgcaggtgca ggaggagctg gctgttcagc atcggctgta ggtagtggta 3180
tcttgaaagg acgatagggt ttgatctaga gatttttatt ttgtctaatt actggtaatg 3240
atggcctcat gcacgctgtt gaacacgctg tacaacatca ctgttgaaga tgatacct 3298
<210> 8
<211> 502
<212> PRT
<213> 来自肺炎克雷伯杆菌 ATCC25597的AroY;
<400> 8
Met Thr Ala Pro Ile Gln Asp Leu Arg Asp Ala Ile Ala Leu Leu Gln
1 5 10 15
Gln His Asp Asn Gln Tyr Leu Glu Thr Asp His Pro Val Asp Pro Asn
20 25 30
Ala Glu Leu Ala Gly Val Tyr Arg His Ile Gly Ala Gly Gly Thr Val
35 40 45
Lys Arg Pro Thr Arg Ile Gly Pro Ala Met Met Phe Asn Asn Ile Lys
50 55 60
Gly Tyr Pro His Ser Arg Ile Leu Val Gly Met His Ala Ser Arg Gln
65 70 75 80
Arg Ala Ala Leu Leu Leu Gly Cys Glu Ala Ser Gln Leu Ala Leu Glu
85 90 95
Val Gly Lys Ala Val Lys Lys Pro Val Ala Pro Val Val Val Pro Ala
100 105 110
Ser Ser Ala Pro Cys Gln Glu Gln Ile Phe Leu Ala Asp Asp Pro Asp
115 120 125
Phe Asp Leu Arg Thr Leu Leu Pro Ala His Thr Asn Thr Pro Ile Asp
130 135 140
Ala Gly Pro Phe Phe Cys Leu Gly Leu Ala Leu Ala Ser Asp Pro Val
145 150 155 160
Asp Ala Ser Leu Thr Asp Val Thr Ile His Arg Leu Cys Val Gln Gly
165 170 175
Arg Asp Glu Leu Ser Met Phe Leu Ala Ala Gly Arg His Ile Glu Val
180 185 190
Phe Arg Gln Lys Ala Glu Ala Ala Gly Lys Pro Leu Pro Ile Thr Ile
195 200 205
Asn Met Gly Leu Asp Pro Ala Ile Tyr Ile Gly Ala Cys Phe Glu Ala
210 215 220
Pro Thr Thr Pro Phe Gly Tyr Asn Glu Leu Gly Val Ala Gly Ala Leu
225 230 235 240
Arg Gln Arg Pro Val Glu Leu Val Gln Gly Val Ser Val Pro Glu Lys
245 250 255
Ala Ile Ala Arg Ala Glu Ile Val Ile Glu Gly Glu Leu Leu Pro Gly
260 265 270
Val Arg Val Arg Glu Asp Gln His Thr Asn Ser Gly His Ala Met Pro
275 280 285
Glu Phe Pro Gly Tyr Cys Gly Gly Ala Asn Pro Ser Leu Pro Val Ile
290 295 300
Lys Val Lys Ala Val Thr Met Arg Asn Asn Ala Ile Leu Gln Thr Leu
305 310 315 320
Val Gly Pro Gly Glu Glu His Thr Thr Leu Ala Gly Leu Pro Thr Glu
325 330 335
Ala Ser Ile Trp Asn Ala Val Glu Ala Ala Ile Pro Gly Phe Leu Gln
340 345 350
Asn Val Tyr Ala His Thr Ala Gly Gly Gly Lys Phe Leu Gly Ile Leu
355 360 365
Gln Val Lys Lys Arg Gln Pro Ala Asp Glu Gly Arg Gln Gly Gln Ala
370 375 380
Ala Leu Leu Ala Leu Ala Thr Tyr Ser Glu Leu Lys Asn Ile Ile Leu
385 390 395 400
Val Asp Glu Asp Val Asp Ile Phe Asp Ser Asp Asp Ile Leu Trp Ala
405 410 415
Met Thr Thr Arg Met Gln Gly Asp Val Ser Ile Thr Thr Ile Pro Gly
420 425 430
Ile Arg Gly His Gln Leu Asp Pro Ser Gln Thr Pro Glu Tyr Ser Pro
435 440 445
Ser Ile Arg Gly Asn Gly Ile Ser Cys Lys Thr Ile Phe Asp Cys Thr
450 455 460
Val Pro Trp Ala Leu Lys Ser His Phe Glu Arg Ala Pro Phe Ala Asp
465 470 475 480
Val Asp Pro Arg Pro Phe Ala Pro Glu Tyr Phe Ala Arg Leu Glu Lys
485 490 495
Asn Gln Gly Ser Ala Lys
500
<210> 9
<211> 5502
<212> DNA
<213> 肺炎克雷伯杆菌342的aroY基因加两千碱基的周围DNA序列;
<400> 9
gcgacgccga ctgggcgatc cgtgaactgc tggcgcgtat gacccagcgt ctgcagggct 60
gtgaaaccat agaggatgtg attaaggtgg cggagctgtt cgcgccgaac atcgccccga 120
cgatccccgg taaactgtat attctggata ccgatccatg gcagatgcgc tgcgtggcgc 180
agtggctgtc gcccgccggg gagacgacgt cctttgctcc cgacgactgc tgggcgatac 240
ggcggggact cagccatccg ccggtgcagg gtgagcccga tatcacctgc tatcatctgc 300
cggaggcgca cgccggccag tcgctctgcg taccgctcat cgcccagggc gaagcgatcg 360
gtctgctgag ctttcagaac gtcaccgcca gtgacgcccc ttcccgggct tacctggagc 420
tgatggccga agcgctgggg ctggcgctcg ccaatcagcg tttacgcagc gccctgctgg 480
aaaaagcgtt gttcgattcg ctgaccggcc tgcgtaaccg ccatcatctt gatgaagcgc 540
tgcactcgca gatggcgctg gcggtccata cccacacccc gctgagctgc ctgatgatcg 600
acatcgatca cttcaaagcc atcaatgacc gctacggcca tgaagccggg gatctggtga 660
ttaagagcgt cgcgaccatt gtgcagcgcg cggtgcgcga tatcggcatg gctttccgct 720
acggcggcga ggagttttta gtgctgctcc ccgggattga cgaagccggg gcgcaccagt 780
gcgccagcga gatctacacc caggtgcaca atatgacgct gcgcgatggc ctgacggaga 840
taggccaggt ggatgtgtcg attggcatcg ccagctaccc gcagcacacc caaagcgaca 900
gcctgctgcg cgcagcggac gccgcgctgt accgggcgaa agagctgggc cgttcaagga 960
ttgtcagctt tggccgcctg aagacccgct aagcgggatt attgctcagc ggcattaagc 1020
agcgagataa ctttccgcac caccgccgaa cggaaatggc ggtggtaaac catgctcagc 1080
tcactctccg ccagccgatc ctgaagatcg atatacacca cgttatcaag gcgtaaggcc 1140
ctcgccgaga ccggcaccag cgccaccccc accccggcgg agaccaggct aatcatcgag 1200
gtgacatcgt taattcgctg caccacctgc ggcgtaaaac ccgcgacgcg acaggcgtca 1260
ataaatacct gctccagtcc ggtgccctgc ggatcgtcaa gcgagatcca gttgtcagtg 1320
cgcaacgagg ccagattgag cgcccccacg cctgccagcg gatgctgttg ataaagcgcc 1380
aggcaaagtt tttcccgcac aaatggcctg accaccagcg cgtccggcgg tgacgccagc 1440
ggcgcgcgga tgatggcgat atccagacgc agatccagca gcgcttcgta gagcatttgc 1500
acatccccct gcaccagcga cagctcaatc cccggccagt cagcgcgcag ctcgcgcagg 1560
agccccggca gtttgctgtc atacatcgca ctggagacat agcccagatg caatcgcccc 1620
tgctcgcctc gtgcggtgcg ctgggcgtcc aggaccgcct gatcggccat ctccagcgcc 1680
agccgcgtct tctgcaagaa ggcctcgccc gcggcggtga gggtcaggcg ccggttagcg 1740
cgggagaaga gcaccacgcc caggcgctgc tcgagttgtt taatctgctg gctgagggcg 1800
ggctgggcga tatgtaaccg ctctgccgcc cgatgcatat gtagttcttc agcaacgacc 1860
acaaaatggc gtaacgctcg caaggacatg gccggactcc gcggagtaaa ttgataataa 1920
aaatgttatc aataaagcat gaatgatgca attgataacc attagcctgc gagcatactg 1980
tgcgcatcga cacgctaagg agaacatcat gaccgcaccg attcaggatc tgcgcgacgc 2040
tatcgcgctg ctgcaacagc atgacaatca gtacctcgaa accgatcatc cggttgaccc 2100
taacgctgag ctggccggcg tctatcgcca catcggcgcg ggcggcaccg tgaagcgccc 2160
cacgcgcatc ggcccggcga tgatgtttaa caatattaag ggctatccgc actcgcgcat 2220
tctggtgggc atgcacgcca gccgccagcg ggccgcgctg ctgctgggct gcgaagcctc 2280
acagctggcg ctggaggtag gcaaagcggt gaaaaaaccg gtcgcgccgg tggtcgttcc 2340
ggccagcagc gccccctgtc aggaacaggt ctttctggcc gacgatccgg attttgattt 2400
gcgcaccctg ctcccggcgc ccaccaacac cccgatcgac gccggtccct tcttctgcct 2460
gggcctggcg ctggccagcg atcccgacga cgcctcgctc accgacgtca ccatccaccg 2520
cttgtgcgtc cagggccggg atgagctgtc gatgtttctc gccgctggcc gccatatcga 2580
agtgtttcgt cagaaagccg aggccgctgg caaaccgctg ccgataacca tcaatatggg 2640
actcgatccg gctatctata tcggcgcctg ctttgaagcg ccgaccacac gtttggctat 2700
aacgaactgg gcgtcgccgg tgcgctgcgt cagcgtccgg tagagctggt acagggcgtc 2760
agcgtcccgg agaaagccat cgctcgcgcc gagatcgtta tcgaagggga actgctgccg 2820
ggggtacgcg tcagagaaga tcagcacaca acagcggcca tgcgatgccg gaatttcctg 2880
gttactgcgg cggcgccaat ccgtcgctgc cggtcattaa agtcaaagcg gtgaccatgc 2940
gaaacaatgc gattctgcag acgctggtag ggccgggcga agagcatacc accctcgccg 3000
gattgccaac ggaagccagt atctggaatg ctgtcgaggc tgctatcccg ggctttttac 3060
aaaatgtcta cgcccacacc gcgggcggcg gtaaattcct cgggatcctg caggtgaaaa 3120
aacgccagcc cgccgacgaa gggcgtcagg ggcaggccgc gttgctggcg ctggcgacct 3180
attccgagct gaaaaatatc attctggtcg atgaagatgt cgatatcttt gacagcgacg 3240
atatcctgtg ggccatgacc acccgcatgc agggggatgt cagcatcacg acgatcccag 3300
gcattcgtgg tcaccagctg gatccttccc agaccccggc ctacagcccg tcgatccgcg 3360
gagagggtat cagttgcaag acgattttcg attgcacggt gccgtgggcg ctaaaatcac 3420
acttcgagcg cgcaccgttt gccgatgtcg atccgcgtcc gtttgcgccg gagtattttg 3480
cccggctgga aaaaaaccac ggtcagtaaa atcaggtgat agccgccgga gcacggcggc 3540
atcttccggg ccagcatcac ctgcagcggg tggctgacgc agggttagtt gatcgcggcg 3600
gagaggtctt ttttcacctg ctcacgctgc tcgggggtca acacctggct cacgtcgaag 3660
tagtatttca cacgataata ccgcacctgc tggtccagct ggccaaaggc ggccagctgc 3720
tgtttgacct tagcgtcatc ccatttcccg gagtgaataa cgtctgccag ggcaccatcc 3780
tgatagccgc tgatttaatc tggcttacat tgttttcgaa tccctgacgc agcgcctgga 3840
ttttggcgac ctgctcttca ctcagcttca ggtgctggac gaccggatcc tgcgagacag 3900
acggtatatc ggcggaggtc gacgcctggc tggctgccgt aaagcaggtg gtcagcgcaa 3960
tggcgagcag ggtgttacgc aagcgagtat tcacagtgaa tgatccttca aaaaagaaaa 4020
tgagaggcga ttatcactgc gctaataaag actatctgta acaaagggtt aatttaaaac 4080
tggataaaaa aaggatggta agaaacagaa atcagatccc gggtcagcag cacagaaaga 4140
tatattcatc cttccagtaa cggccctgtc caatgatatc cccggcggcg ctgattaact 4200
gtttttgctt ttggtttcaa tcccctcaac gatcacatgg ctggtcaggg tatgaataga 4260
ttgcaacagc ccgggaaaag cggggtcgtt ttctttatcc cagaagtaat ctttatccac 4320
tttgacgcaa tcgaagcgga agcgctccag cagcgggaag gacgtcgtcc gcggccaaaa 4380
tcatccagcc agaccgggca aagcgccgcc agcgtgctca gcgccgtcag ctcacgtccg 4440
gcgataaact cgtgaaagtt ctcatttatt tccagagcaa tgtgtttaca ggagcgcagg 4500
aaatcacaga gatagcgatc cgtcagaata aagtggctta ataaatcatc aatattcagc 4560
gatatcggtt tattatcgac ccgggcgaaa tcaaatacag aaagtaactc gatttgccga 4620
ataaatagag caagcttatc gcgttcggta agcgtggaga aagaaaacgt cgacgcagag 4680
gtggtatttt gtgcgggtgc tataatatct ttagtaagca actcccacga gtggtagctg 4740
ccgtcatcgc taatagcggg ctccagaacg aagcgataag aggtattttt cacgttttct 4800
tcaaccattt aaaaaatacc aaaaataaga aagggttaag catgtcatat attttccgcc 4860
aacaaaaata gtttaaagtg atcgataata atcattcgat agttaaaaac tatcaagata 4920
taatttattg atcggtaaat tgaattaata taaattagcc actgccgtaa ctccctctga 4980
aaagtcaatt aaaatattgt ttcaaaccag ccagttacca gagtattctg cgtaaagcct 5040
ggtcgtctca cgctttgtgc tgccaggtaa aaaaagagag gggtaataaa aatgaaaaat 5100
acaagccgcc agttttagtc atatcattat gccgaatatg aataacgctg cgctgaggcg 5160
ccgcttcgcc tggcatgcca tgagtcctca acaaaaaagt gtgactcagt cgacaaaacg 5220
tcatattttc ccgctatcct gcagcgaaga agagtgaagt ggatgacagg cagtgaaaaa 5280
aataaacgtg attccgctgg ggctgatgct attcatgctc atcgccagcg catggctggg 5340
ccctgcgccg cggcacaccg gcagcatgca gtgcgtttgg tttgacgggg caatggtgag 5400
ctgcctgccg aagcaacgac tgggcgaagg ctcgccgcat catttactgg tcagacgata 5460
aaccggtact cgccgggtgg tgttgaacag attatcgctg gc 5502
<210> 10
<211> 4629
<212> DNA
<213> 来自贝氏不动杆菌ADP1的catA基因的DNA序列,包括410碱基的上游序列和两个下游开放阅读框;
<400> 10
atctgctcga ccatagtaat gatcacatta tgagctaaat ttacttttta aaatttaaat 60
atattatata tatttgaatt ttattgtttt attttaattt ttagcttaga agtttttatt 120
aagatttatt tttaaattag atgtcgaaaa aattagtata ccaaaaaagc atgaaaacat 180
actctcttag gaattggagt cgccatgagt ttcagataca gttgatcagt atggaaggta 240
tagaaacgac tatcgaaata aataagtttg tggtgtgtga agcaaggtaa agctcaaggc 300
tgaggcaaac caagcaaagg ttaattgaac cgatatgcac aacacattca acgatagcgt 360
cgacagataa gtttatcaaa tgatgttttg gcgatttcaa ggagaaagcc atggaagtta 420
aaatattcaa tactcaggat gtgcaagatt ttttacgtgt tgcaagcgga cttgagcaag 480
aaggtggcaa tccgcgtgta aagcagatca tccatcgtgt gctttcagat ttatataaag 540
ccattgaaga tttgaatatc acttcagatg aatactgggc aggtgtggca tatttaaatc 600
agctaggtgc caatcaagaa gctggtttac tctcgccagg cttgggtttt gaccattacc 660
tcgatatgcg tatggatgcc gaagatgccg cactaggtat tgaaaatgcg acaccacgta 720
ccattgaagg cccgctatac gtggcaggtg cgcctgaatc ggtaggttat gcgcgcatgg 780
atgacggaag tgatccaaat ggtcataccc tgattctaca tggcacgatc tttgatgcag 840
atggaaaacc tttacccaat gccaaagttg aaatctggca tgccaatacc aaaggctttt 900
attcacactt cgacccaaca ggcgagcagc aggcgttcaa tatgcgccgt agtattatta 960
ccgatgaaaa cggtcagtat cgcgttcgta ccattttgcc tgcgggttat ggttgcccac 1020
cagaaggtcc aacgcaacag ttgctgaatc agttgggccg tcatggtaac cgccctgcgc 1080
acattcacta ttttgtttct gccgatggac accgcaaact aactacgcaa attaatgtgg 1140
ctggcgatcc gtacacctat gacgactttg cttatgcaac ccgtgaaggc ttggtggttg 1200
atgcagtgga acacaccgat cctgaagcca ttaaggccaa tgatgttgaa ggcccattcg 1260
ctgaaatggt tttcgatcta aaattgacgc gtttggttga tggtgtagat aaccaagttg 1320
ttgatcgtcc acgtctagcg gtgtaataca ccaaaatggt tcaaaattat caggcgagtg 1380
atcatgatca ctggcctgtt tttatttcag ggaagggtgg agacaattac gtggacaatc 1440
aaatcattca ggaaaccgta gataaaattt taagcgtatt gccgaatcag gctgggcaat 1500
tggcacgctt ggttcgtctg atgcagtttg cttgtgaccc caccattacc gtcattggta 1560
aatataatca tggtaaaagc cgactactca atgagctgat cgggacagat attttttctg 1620
ttgccgataa acgagagacg attcaactgg ccgaacataa acaagatcag gtgcgttggt 1680
tggatgcacc cggactcgat gcagatgttg cggcagtgga tgatcgtcat gcttttgaag 1740
cagtctggac acaggcagat attcgccttt ttgtgcattc agtccgagaa ggcgaactcg 1800
atgcaactga gcatcatctt ttacaacaac ttattgaaga tgcagaccat agccggcgcc 1860
aaaccatact ggtcttgacc cagatagatc agataccgga tcagacaatt ttaacccaga 1920
ttaaaacctc aattgcacag caggtaccca aactcgatat ttgggctgtt tcggccactc 1980
gccaccgtca gggtattgaa aatggaaaaa ccttgctgat cgaaaaaagt ggaatcggcg 2040
cgttacgaca tacacttgag caggcacttg ctcaggttcc atctgcacga acgtatgaaa 2100
agaatagatt gctgtctgac ttgcatcatc aacttaagca gttattactc gatcaaaaac 2160
atgtacttca gcaactacaa cagacacagc agcagcaatt gcatgacttt gatacaggac 2220
tcatcaacat actcgataag attcgagtag atcttgagcc cattgtaaat atagatggtc 2280
aagaccaagc actcaatcca gattcatttg ccacgatgtt taaaaataca gcagccaagc 2340
agcaacgtgc caaagtgcag attgcttact cacgtgcctg tatcgagatc aatagccatc 2400
tcatacgtca cggtgtggtg ggtttacccg cagagcaaca aaccacgata aaaagtattg 2460
atacggtcat tgttgcggtt tttggaattt cagtgaaatt tcgcgatcag ctacgtgcat 2520
tgttttatac cgataccgaa cgacaacgct tgcaaagaga gtttcgattt tattttgaaa 2580
agtcagcagg ccgaatgatt ttagctgcca agattgagca gacaatgcgg cagcaagggt 2640
gtattcaaaa tgcaatgatg gcgttgcaac agatggagag tgcagcatga ccagcggcgg 2700
acacattcaa ttgtttatcg aacacacccg gcagattgcg actgcccaag gggatataca 2760
gttggcattg caatcgatgc agcaatggcg cgaagcattt gctacagcat taaaacaaaa 2820
tacctttgat ttaacgggct ggtcaccgca gacaaagatc gccaatcaac tcaagcaatt 2880
taaccataag cttacaacgc atgtatcgaa ttgggatacc gaatggcata cttttagtgc 2940
tgctcaatcg gttgcagaag tatttcatga tcgggtgatg ttgcttgtat tcggtaagtt 3000
taatgccgga aagagttcat tgtgtaactt actggccgaa tgctttcgtt ctcacgaaca 3060
aaccgtgcaa tattttcatg ttcaaaatga acagatattt tataccgaat ctcacttacg 3120
cgaaggtgca accgagacga cagcgcaact acagggcgta tgtctgggtg aaaaacttat 3180
tttgctagat acaccaggtt tgcattctgg tactcagaaa aatgcagcgc tcacacaaaa 3240
atttatcgac agtgcagatg gtgtgctgtg gctcagtagc gcaacttcac cgggtcaggt 3300
gcaagagcta gatgcactgg ggcgcgagtt aaagcgtcat aaacctttat ttcctgttat 3360
tacccgaagc gattttgtcg aagaagatga aattgatggt gagctatgta cagtgctttg 3420
caataaaaat tcagaacaac gtgcgttgca agagtctgat gtattgatgc gtgcgaaaga 3480
aaaactgcac atatgcaagt ggatgtgagt ttattaaagc cgcccgtgtc cgtttcaact 3540
caaatggcgc gtgaagcaga tatgaaccca caagccatga acgaggctgg ttttgagcga 3600
ttatttgcag cacttttggc tcttattgag cctgctttgc gctataagca gcgtaaacct 3660
gccgaagtat tgttgcattt tttgcaagaa catatcattg aaggtttaag gttttacctg 3720
caacccgatc tagagcaaat acaacaggac ctcaaacagg ctcaagatga tttacgacag 3780
ctacacaccg atttagccga ggcagtctgg cgtagcgtat tgcctgagct accacaactt 3840
cttgagcaac atgcaagtac acaaaatatt gatgccgtag tgaacagttt gaacgagtgg 3900
ataaacgtcg cattcgaaca acagcttgca attcagcttg atgcttatgg tttaaatttg 3960
gattcgctta gcaagatcga aaaaaccgaa aaaatgcagt atgaacgcat tgcgggaatg 4020
gtggtgcatg atggcttgta cacgactctc acgcagcaga ttcaacaagc tgtcaaagct 4080
tctacgagtg aattgattga tcagtgtcag gctcaacttg agcagtcaat caaacatgtt 4140
caaacactcg atgaaacctt catcgattac agcgcagcac tcgatcaact cagccaagcg 4200
ctacgcattg aataaagagc agtaaatttt tcagacatat tttattcgat gagtggcctg 4260
atatggtgcg ttgcaaacac ctcctgtaca caggcgagaa ttttaggaat gtaattactg 4320
tggtccatat ttcgcaccgc gagtgaaatt gggctatagg catcatcatc taaaattgga 4380
atataaagta gattcttcac cccaatatcc atggcagacg ccggtacgat gcagacgcct 4440
tcacctgctg ccaccaagcc gagtgccagt tgaatttctc gaatttcggt gagtttggat 4500
ggtactaggc ctagttcggt aaagagtgac tgaataaagg tcgcaaaatt gggcttttga 4560
gagactgggt acagcagcat cggttcatca ataatttgag agagatgaac ccctgttgct 4620
gcaaactga 4629
<210> 11
<211> 311
<212> PRT
<213> 来自贝氏不动杆菌ADP1的CatA;
<400> 11
Met Glu Val Lys Ile Phe Asn Thr Gln Asp Val Gln Asp Phe Leu Arg
1 5 10 15
Val Ala Ser Gly Leu Glu Gln Glu Gly Gly Asn Pro Arg Val Lys Gln
20 25 30
Ile Ile His Arg Val Leu Ser Asp Leu Tyr Lys Ala Ile Glu Asp Leu
35 40 45
Asn Ile Thr Ser Asp Glu Tyr Trp Ala Gly Val Ala Tyr Leu Asn Gln
50 55 60
Leu Gly Ala Asn Gln Glu Ala Gly Leu Leu Ser Pro Gly Leu Gly Phe
65 70 75 80
Asp His Tyr Leu Asp Met Arg Met Asp Ala Glu Asp Ala Ala Leu Gly
85 90 95
Ile Glu Asn Ala Thr Pro Arg Thr Ile Glu Gly Pro Leu Tyr Val Ala
100 105 110
Gly Ala Pro Glu Ser Val Gly Tyr Ala Arg Met Asp Asp Gly Ser Asp
115 120 125
Pro Asn Gly His Thr Leu Ile Leu His Gly Thr Ile Phe Asp Ala Asp
130 135 140
Gly Lys Pro Leu Pro Asn Ala Lys Val Glu Ile Trp His Ala Asn Thr
145 150 155 160
Lys Gly Phe Tyr Ser His Phe Asp Pro Thr Gly Glu Gln Gln Ala Phe
165 170 175
Asn Met Arg Arg Ser Ile Ile Thr Asp Glu Asn Gly Gln Tyr Arg Val
180 185 190
Arg Thr Ile Leu Pro Ala Gly Tyr Gly Cys Pro Pro Glu Gly Pro Thr
195 200 205
Gln Gln Leu Leu Asn Gln Leu Gly Arg His Gly Asn Arg Pro Ala His
210 215 220
Ile His Tyr Phe Val Ser Ala Asp Gly His Arg Lys Leu Thr Thr Gln
225 230 235 240
Ile Asn Val Ala Gly Asp Pro Tyr Thr Tyr Asp Asp Phe Ala Tyr Ala
245 250 255
Thr Arg Glu Gly Leu Val Val Asp Ala Val Glu His Thr Asp Pro Glu
260 265 270
Ala Ile Lys Ala Asn Asp Val Glu Gly Pro Phe Ala Glu Met Val Phe
275 280 285
Asp Leu Lys Leu Thr Arg Leu Val Asp Gly Val Asp Asn Gln Val Val
290 295 300
Asp Arg Pro Arg Leu Ala Val
305 310
<210> 12
<211> 1461
<212> DNA
<213> 来自不动杆菌属物种ADP1的quiC;
<400> 12
atgaaattaa cttctttacg cgtatcttta ttggcgctgg gcttggtaac atcaggtttt 60
gctgcggcag aaacttatac tgtagatcgt tatcaggatg atagtgaaaa aggctctttg 120
cgttgggcaa ttgaacaatc taatgcaaat agcgcacaag agaatcagat tctgattcag 180
gctgttggta aggcacctta tgtgatcaag gtggataaac cgttaccacc gattaaatca 240
tctgtaaaaa ttattggtac agaatgggat aaaacgggcg aatttattgc gattgatggt 300
tcaaactata tcaagggcga aggcgaaaaa gcatgtccag gtgcaaatcc aggacaatat 360
ggtaccaatg ttcgtaccat gactttacca ggtttggttc tacaagatgt caatggtgtg 420
accctgaaag gtcttgatgt tcatcgcttc tgtattggtg tactggtaaa tcgttcaagc 480
aataatttga ttcagcataa ccgtatttca aataattacg gtggcgctgg tgtcatgatc 540
acgggtgatg atggtaaagg taacccaacg tctaccacca ccaataacaa caaagtattg 600
gataatgtgt ttattgacaa tggcgatggt cttgaactga cgcgtggagc agcattcaac 660
ctgattgcta acaatctgtt tacatcgacc aaagccaatc cagagccgtc tcaaggcatt 720
gaaattcttt gggggaatga caatgcagtg gtgggtaaca aatttgaaaa ctattcagat 780
ggtctacaaa tcaactgggg taaacgtaat tacatcgctt ataacgaatt gaccaataac 840
tctttgggtt tcaatcttac aggtgatgga aacatcttcg atagtaacaa agtgcatggc 900
aatcgtattg gtatcgcaat tcgttctgaa aaagatgcaa atgcacgtat cacacttacc 960
aaaaatcaga tttgggataa tggtaaagat atcaaacgct gtgaggctgg tggttcatgt 1020
gttccaaacc aacgtttagg tgcaattgta tttggtgttc ctgcgcttga gcatgaaggt 1080
tttgtaggct ctcgtggtgg cggtgtagtc attgaacctg caaaattaca aaaaacatgt 1140
acacagccaa atcaacaaaa ctgtaatgcc attccgaacc aaggtattca ggcacctaaa 1200
ctgactgtca gtaaaaaaca acttacagtt gaagttaaag gaacaccaaa ccagcgttac 1260
aacgtagaat tttttggaaa tcgtaatgca tcttcttccg aagctgagca atatttaggt 1320
tcaattgttg tagtgacaga tcatcaaggt cttgcaaaag caaactgggc accaaaagtc 1380
agcatgccat ctgttactgc gaatgtaact gatcacttgg gcgccacttc agagttaagt 1440
tctgcagtga aaatgagata a 1461
<210> 13
<211> 1461
<212> DNA
<213> 来自不动杆菌属物种ADP1的经密码子优化的quiC基因;
<400> 13
atgaaactga ccagcctgcg tgttagcctg ctggcactgg gtctggttac cagcggtttt 60
gcagcagcag aaacctatac cgttgatcgt tatcaggatg atagcgaaaa aggtagcctg 120
cgttgggcaa ttgaacagag caatgcaaat agcgcacaag aaaaccagat tctgattcag 180
gcagttggta aagcaccgta tgttatcaaa gttgataaac cgctgcctcc gattaaaagc 240
agcgttaaaa tcattggcac cgagtgggat aaaaccggtg aatttattgc aattgatggc 300
agcaactata tcaaaggcga aggtgaaaaa gcatgtccgg gtgcaaatcc gggtcagtat 360
ggcaccaatg ttcgtaccat gaccctgcct ggtctggttc tgcaagatgt taatggtgtt 420
accctgaaag gtctggatgt tcatcgtttt tgtattggtg ttctggttaa tcgcagcagc 480
aataacctga ttcagcataa tcgtatcagc aacaattatg gtggtgccgg tgttatgatt 540
accggtgatg atggtaaagg taatccgacc agcaccacca ccaataataa caaagttctg 600
gataacgtgt tcatcgataa tggtgatggt ctggaactga cccgtggtgc agcatttaat 660
ctgattgcaa ataacctgtt taccagcaca aaagccaatc cggaaccgag ccagggtatt 720
gaaattctgt ggggtaatga taatgccgtg gtgggtaaca aattcgaaaa ctattcagat 780
ggcctgcaaa tcaattgggg taaacgtaac tatatcgcct ataacgaact gaccaataac 840
agcctgggtt tcaatctgac aggtgatggt aacattttcg acagcaataa agtgcatggt 900
aaccgtattg gtattgccat tcgtagtgaa aaagatgcca atgcacgtat taccctgacc 960
aaaaatcaga tttgggataa cggcaaagat atcaaacgtt gtgaagccgg tggtagctgt 1020
gttccgaatc agcgtctggg tgcaattgtt tttggtgttc cggcactgga acatgaaggt 1080
tttgttggta gccgtggcgg tggtgttgtt attgaaccgg caaaactgca aaaaacctgc 1140
acccagccga accagcagaa ttgtaatgca attcctaatc agggtattca ggcaccgaaa 1200
ctgacagtta gcaaaaaaca gctgaccgtt gaagttaaag gcacccctaa tcagcgttat 1260
aatgtggaat tttttggcaa tcgtaatgcc agcagcagcg aagcagaaca gtatctgggt 1320
agcattgttg ttgttaccga tcatcagggt ctggcaaaag caaattgggc tccgaaagtt 1380
agcatgccga gcgttaccgc aaatgtgaca gatcatctgg gtgcgaccag cgaactgagc 1440
agcgcagtta aaatgcgtta a 1461
<210> 14
<211> 486
<212> PRT
<213> 来自不动杆菌属物种ADP1的QuiC;
<400> 14
Met Lys Leu Thr Ser Leu Arg Val Ser Leu Leu Ala Leu Gly Leu Val
1 5 10 15
Thr Ser Gly Phe Ala Ala Ala Glu Thr Tyr Thr Val Asp Arg Tyr Gln
20 25 30
Asp Asp Ser Glu Lys Gly Ser Leu Arg Trp Ala Ile Glu Gln Ser Asn
35 40 45
Ala Asn Ser Ala Gln Glu Asn Gln Ile Leu Ile Gln Ala Val Gly Lys
50 55 60
Ala Pro Tyr Val Ile Lys Val Asp Lys Pro Leu Pro Pro Ile Lys Ser
65 70 75 80
Ser Val Lys Ile Ile Gly Thr Glu Trp Asp Lys Thr Gly Glu Phe Ile
85 90 95
Ala Ile Asp Gly Ser Asn Tyr Ile Lys Gly Glu Gly Glu Lys Ala Cys
100 105 110
Pro Gly Ala Asn Pro Gly Gln Tyr Gly Thr Asn Val Arg Thr Met Thr
115 120 125
Leu Pro Gly Leu Val Leu Gln Asp Val Asn Gly Val Thr Leu Lys Gly
130 135 140
Leu Asp Val His Arg Phe Cys Ile Gly Val Leu Val Asn Arg Ser Ser
145 150 155 160
Asn Asn Leu Ile Gln His Asn Arg Ile Ser Asn Asn Tyr Gly Gly Ala
165 170 175
Gly Val Met Ile Thr Gly Asp Asp Gly Lys Gly Asn Pro Thr Ser Thr
180 185 190
Thr Thr Asn Asn Asn Lys Val Leu Asp Asn Val Phe Ile Asp Asn Gly
195 200 205
Asp Gly Leu Glu Leu Thr Arg Gly Ala Ala Phe Asn Leu Ile Ala Asn
210 215 220
Asn Leu Phe Thr Ser Thr Lys Ala Asn Pro Glu Pro Ser Gln Gly Ile
225 230 235 240
Glu Ile Leu Trp Gly Asn Asp Asn Ala Val Val Gly Asn Lys Phe Glu
245 250 255
Asn Tyr Ser Asp Gly Leu Gln Ile Asn Trp Gly Lys Arg Asn Tyr Ile
260 265 270
Ala Tyr Asn Glu Leu Thr Asn Asn Ser Leu Gly Phe Asn Leu Thr Gly
275 280 285
Asp Gly Asn Ile Phe Asp Ser Asn Lys Val His Gly Asn Arg Ile Gly
290 295 300
Ile Ala Ile Arg Ser Glu Lys Asp Ala Asn Ala Arg Ile Thr Leu Thr
305 310 315 320
Lys Asn Gln Ile Trp Asp Asn Gly Lys Asp Ile Lys Arg Cys Glu Ala
325 330 335
Gly Gly Ser Cys Val Pro Asn Gln Arg Leu Gly Ala Ile Val Phe Gly
340 345 350
Val Pro Ala Leu Glu His Glu Gly Phe Val Gly Ser Arg Gly Gly Gly
355 360 365
Val Val Ile Glu Pro Ala Lys Leu Gln Lys Thr Cys Thr Gln Pro Asn
370 375 380
Gln Gln Asn Cys Asn Ala Ile Pro Asn Gln Gly Ile Gln Ala Pro Lys
385 390 395 400
Leu Thr Val Ser Lys Lys Gln Leu Thr Val Glu Val Lys Gly Thr Pro
405 410 415
Asn Gln Arg Tyr Asn Val Glu Phe Phe Gly Asn Arg Asn Ala Ser Ser
420 425 430
Ser Glu Ala Glu Gln Tyr Leu Gly Ser Ile Val Val Val Thr Asp His
435 440 445
Gln Gly Leu Ala Lys Ala Asn Trp Ala Pro Lys Val Ser Met Pro Ser
450 455 460
Val Thr Ala Asn Val Thr Asp His Leu Gly Ala Thr Ser Glu Leu Ser
465 470 475 480
Ser Ala Val Lys Met Arg
485
<210> 15
<211> 9462
<212> DNA
<213> 质粒pAC21;
<220>
<221> misc_feature
<222> (5924)..(5924)
<223> n是a、c、g或t
<400> 15
gttgacagta agacgggtaa gcctgttgat gataccgctg ccttactggg tgcattagcc 60
agtctgaatg acctgtcacg ggataatccg aagtggtcag actggaaaat cagagggcag 120
gaactgctga acagcaaaaa gtcagatagc accacatagc agacccgcca taaaacgccc 180
tgagaagccc gtgacgggct tttcttgtat tatgggtagt ttccttgcat gaatccataa 240
aaggcgcctg tagtgccatt tacccccatt cactgccaga gccgtgagcg cagcgaactg 300
aatgtcacga aaaagacagc gactcaggtg cctgatggtc ggagacaaaa ggaatattca 360
gcgatttgcc cgagcttgcg agggtgctac ttaagccttt agggttttaa ggtctgtttt 420
gtagaggagc aaacagcgtt tgcgacatcc ttttgtaata ctgcggaact gactaaagta 480
gtgagttata cacagggctg ggatctattc tttttatctt tttttattct ttctttattc 540
tataaattat aaccacttga atataaacaa aaaaaacaca caaaggtcta gcggaattta 600
cagagggtct agcagaattt acaagttttc cagcaaaggt ctagcagaat ttacagatac 660
ccacaactca aaggaaaagg actagtaatt atcattgact agcccatctc aattggtata 720
gtgattaaaa tcacctagac caattgagat gtatgtctga attagttgtt ttcaaagcaa 780
atgaactagc gattagtcgc tatgacttaa cggagcatga aaccaagcta attttatgct 840
gtgtggcact actcaacccc acgattgaaa accctacaag gaaagaacgg acggtatcgt 900
tcacttataa ccaatacgct cagatgatga acatcagtag ggaaaatgct tatggtgtat 960
tagctaaagc aaccagagag ctgatgacga gaactgtgga aatcaggaat cctttggtta 1020
aaggctttga gattttccag tggacaaact atgccaagtt ctcaagcgaa aaattagaat 1080
tagtttttag tgaagagata ttgccttatc ttttccagtt aaaaaaattc ataaaatata 1140
atctggaaca tgttaagtct tttgaaaaca aatactctat gaggatttat gagtggttat 1200
taaaagaact aacacaaaag aaaactcaca aggcaaatat agagattagc cttgatgaat 1260
ttaagttcat gttaatgctt gaaaataact accatgagtt taaaaggctt aaccaatggg 1320
ttttgaaacc aataagtaaa gatttaaaca cttacagcaa tatgaaattg gtggttgata 1380
agcgaggccg cccgactgat acgttgattt tccaagttga actagataga caaatggatc 1440
tcgtaaccga acttgagaac aaccagataa aaatgaatgg tgacaaaata ccaacaacca 1500
ttacatcaga ttcctaccta cgtaacggac taagaaaaac actacacgat gctttaactg 1560
caaaaattca gctcaccagt tttgaggcaa aatttttgag tgacatgcaa agtaagcatg 1620
atctcaatgg ttcgttctca tggctcacgc aaaaacaacg aaccacacta gagaacatac 1680
tggctaaata cggaaggatc tgaggttctt atggctcttg tatctatcag tgaagcatca 1740
agactaacaa acaaaagtag aacaactgtt caccgttaga tatcaaaggg aaaactgtcc 1800
atatgcacag atgaaaacgg tgtaaaaaag atagatacat cagagctttt acgagttttt 1860
ggtgcattta aagctgttca ccatgaacag atcgacaatg taacagatga acagcatgta 1920
acacctaata gaacaggtga aaccagtaaa acaaagcaac tagaacatga aattgaacac 1980
ctgagacaac ttgttacagc tcaacagtca cacatagaca gcctgaaaca ggcgatgctg 2040
cttatcgaat caaagctgcc gacaacacgg gagccagtga cgcctcccgt ggggaaaaaa 2100
tcatggcaat tctggaagaa atagcgcttt cagccggcaa acctgaagcc ggatctgcga 2160
ttctgataac aaactagcaa caccagaaca gcccgtttgc gggcagcaaa acccgttatg 2220
cttgtaaacc gttttgtgaa aaaattttta aaataaaaaa ggggacctct agggtcccca 2280
attaattagt aatataatct attaaaggtc attcaaaagg tcatccaccg gatcaattcc 2340
cctgctcgcg caggctgggt gccaagctct cgggtaacat caaggcccga tccttggagc 2400
ccttgccctc ccgcacgatg atcgtgccgt gatcgaaatc cagatccttg acccgcagtt 2460
gcaaaccctc actgatccgc atgcccgttc catacagaag ctgggcgaac aaacgatgct 2520
cgccttccag aaaaccgagg atgcgaacca cttcatccgg ggtcagcacc accggcaagc 2580
gccgcgacgg ccgaggtctt ccgatctcct gaagccaggg cagatccgtg cacagcacct 2640
tgccgtagaa gaacagcaag gccgccaatg cctgacgatg cgtggagacc gaaaccttgc 2700
gctcgttcgc cagccaggac agaaatgcct cgacttcgct gctgcccaag gttgccgggt 2760
gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc 2820
gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac 2880
gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg 2940
tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga 3000
tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca 3060
tcatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca 3120
tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg 3180
gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg 3240
aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga 3300
gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc 3360
gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag 3420
gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag 3480
aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac 3540
aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg 3600
ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg 3660
gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt 3720
atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg 3780
cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg 3840
tagtcggcaa ataatgtcta acaattcgtt caagccgacg ccgcttcgcg gcgcggctta 3900
actcaagcgt tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc 3960
tgcttttatt atttttaagc gtgcataata agccctacac aaattgggag atatatcatg 4020
aaaggctggc tttttcttgt tatcgcaata gttggcgaag taatcgcaac atccgcatta 4080
aaatctagcg agggctttac taagctgatc cggtggatga ccttttgaat gacctttaat 4140
agattatatt actaattaat tggggaccct agaggtcccc ttttttattt taaaaatttt 4200
ttcacaaaac ggtttacaag catacgttgg ccgattcatt aatgcagctg gcacgacagg 4260
tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat 4320
taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc 4380
ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct tgcatgcctg 4440
caggtcgact ctagaggatc ccccccgccg ccgacagagt aataggtttt acttaatagc 4500
tcttcctgtc ccttccaggc agtgatccgc attccgttct catggcgagg caacatttcg 4560
ggatggaaga taatgttctt tgctacagga aaatcaacaa tatgcgcacc agatgccact 4620
ggcagccgcc cgctgcgcgt tactaactct ataaatgcag ggatctcatc aatgacaaca 4680
tcctgcggac tgtttcctgc cagtcccatg atgatggcga catccgtggc atggcctttg 4740
cccgtcagtg acaacgaccc gtacagatcg accacaatat ggctcgtcgc ggttaataag 4800
ccgctacttt ccagccgatc aataaaactt tttccggcat tcattggccc cacggtatgc 4860
gaactggagg gaccaatccc aattttgaaa atatcgaatg cactaatcat gtgacggaag 4920
atcacttcgc agaataaata aatcctggtg tccctgttga taccgggaag ccctgggcca 4980
acttttggcg aaaatgagac gttgatcggc acgtaagagg ttccaacttt caccataatg 5040
aaataagatc actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag 5100
ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta 5160
aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc 5220
tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct 5280
ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag 5340
acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa 5400
ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca 5460
tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta 5520
ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa 5580
acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc 5640
aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct 5700
tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg 5760
cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc tggtgctacg cctgaataag 5820
tgataataag cggatgaatg gcagaaattc gaaagcaaat tcgacccggt cgtcggttca 5880
gggcagggtc gttaaatagc cgcttatgtc tattgctggt ttantcggta cccggggatc 5940
gcggccgcgg accggatccc atcacatata cctgccgttc actattattt agtgaaatga 6000
gatattatga tattttctga attgtgatta aaaaggcaac tttatgccca tgcaacagaa 6060
actataaaaa atacagagaa tgaaaagaaa cagatagatt ttttagttct ttaggcccgt 6120
agtctgcaaa tccttttatg attttctatc aaacaaaaga ggaaaataga ccagttgcaa 6180
tccaaacgag agtctaatag aatgaggtcg aaaagtaaat cgcgcgggtt tgttactgat 6240
aaagcaggca agacctaaaa tgtgtaaagg gcaaagtgta tactttggcg tcacccctta 6300
catattttag gtcttttttt attgtgcgta actaacttgc catcttcaaa caggagggct 6360
ggaagaagca gaccgctaac acagtacata aaaaaggaga catgaacgat gaacatcaaa 6420
aagtttgcaa aacaagcaac agtattaacc tttactaccg cactgctggc aggaggcgca 6480
actcaagcgt ttgcgaaaga aacgaaccaa aagccatata aggaaacata cggcatttcc 6540
catattacac gccatgatat gctgcaaatc cctgaacagc aaaaaaatga aaaatatcaa 6600
gttcctgaat tcgattcgtc cacaattaaa aatatctctt ctgcaaaagg cctggacgtt 6660
tgggacagct ggccattaca aaacgctgac ggcactgtcg caaactatca cggctaccac 6720
atcgtctttg cattagccgg agatcctaaa aatgcggatg acacatcgat ttacatgttc 6780
tatcaaaaag tcggcgaaac ttctattgac agctggaaaa acgctggccg cgtctttaaa 6840
gacagcgaca aattcgatgc aaatgattct atcctaaaag accaaacaca agaatggtca 6900
ggttcagcca catttacatc tgacggaaaa atccgtttat tctacactga tttctccggt 6960
aaacattacg gcaaacaaac actgacaact gcacaagtta acgtatcagc atcagacagc 7020
tctttgaaca tcaacggtgt agaggattat aaatcaatct ttgacggtga cggaaaaacg 7080
tatcaaaatg tacagcagtt catcgatgaa ggcaactaca gctcaggcga caaccatacg 7140
ctgagagatc ctcactacgt agaagataaa ggccacaaat acttagtatt tgaagcaaac 7200
actggaactg aagatggcta ccaaggcgaa gaatctttat ttaacaaagc atactatggc 7260
aaaagcacat cattcttccg tcaagaaagt caaaaacttc tgcaaagcga taaaaaacgc 7320
acggctgagt tagcaaacgg cgctctcggt atgattgagc taaacgatga ttacacactg 7380
aaaaaagtga tgaaaccgct gattgcatct aacacagtaa cagatgaaat tgaacgcgcg 7440
aacgtcttta aaatgaacgg caaatggtac ctgttcactg actcccgcgg atcaaaaatg 7500
acgattgacg gcattacgtc taacgatatt tacatgcttg gttatgtttc taattcttta 7560
actggcccat acaagccgct gaacaaaact ggccttgtgt taaaaatgga tcttgatcct 7620
aacgatgtaa cctttactta ctcacacttc gctgtacctc aagcgaaagg aaacaatgtc 7680
gtgattacaa gctatatgac aaacagagga ttctacgcag acaaacaatc aacgtttgcg 7740
ccgagcttcc tgctgaacat caaaggcaag aaaacatctg ttgtcaaaga cagcatcctt 7800
gaacaaggac aattaacagt taacaaataa aaacgcaaaa gaaaatgcca atatcctatt 7860
ggcattttct tttatttctt ccatttaaat ggatgcatgc gctagcggag tgtatactgg 7920
cttactatgt tggcactgat gagggtgtca gtgaagtgct tcagcctcgt gagcgggacg 7980
gtcgtaaggt cgttccgctc cacttcactg aacggcaatc cgagggtgtg gatccaatta 8040
aggccacgct gtcatttaaa ttccgttttt ccagttcaaa tgcaattgcc ttcaatgcac 8100
cttcgtagct gtggtgagcc agcggtgctg gctctccccc atttacggat aagaatgcat 8160
tttccgagtt aataccgtcg gcaatacctg acattaatac ttcacagtcg ctggcatcga 8220
gtacggaaaa cttaatcgaa gacgaaccac agttaataac caaaacaacc ggaaattcat 8280
tcatctcttt tctcatcctg agttacggat taaaacagtt tgtatacgat gttcaggatg 8340
gtcagcagac caatcacggt aacaaacacg ttatccagac gaccacggta tttcgccaga 8400
gacggcgctt tacggatggc atacatcggc aacaggcaca gcagggatgc gataatcggt 8460
gcgcccatgg cttcaatcag gtcgaggatg ttcgggttgg cgtaggcaac aacccaggtg 8520
gagcccatga tgaagatcat gctgagagta ttcagtttac ccagcgacac tttggttttg 8580
tcacctttat aaccgaactt cagaatcaga ccattcaagc cttccagcgt ccccagatag 8640
tgaccgaaga aagatttgaa gatagccacg agtgcgatga tggaagccgc atattccagt 8700
gtaatcgcga acgttgtttt ggtaccggtc atggacgcaa agtggttagc cagataagaa 8760
agcactggaa tattctgcgc tttggcttcc gccatgttgg ccggagacag agtaaacagg 8820
cagctaaagg caaagaacat caccactgca accatcagca tgctggcacg agaaatgatt 8880
tgggaacatt tacgttcggt gaagtcgcga ccgaagtctt tctcatactc ttcacgttta 8940
gaaaccacga aggaagagac gattggcgag aagttaaagg agaaaaccat gatggaaatc 9000
cccagccaga cagtgatcag gataccgtca tgaccggtta acgacagcga accgaggtca 9060
acctggtcga taactgcaga gttccagtaa gggatcagcg acaaagaaat cagcaccagg 9120
ctggcgataa acggccatac caggtagctc agggtaccga gctcgaattc actggccgtc 9180
gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca 9240
catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa 9300
cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg 9360
tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag 9420
ttaagccagc cccgacaccc gccaacaccc gctgacgaat tc 9462
<210> 16
<211> 9430
<212> DNA
<213> 质粒pAC19;
<400> 16
gttgacagta agacgggtaa gcctgttgat gataccgctg ccttactggg tgcattagcc 60
agtctgaatg acctgtcacg ggataatccg aagtggtcag actggaaaat cagagggcag 120
gaactgctga acagcaaaaa gtcagatagc accacatagc agacccgcca taaaacgccc 180
tgagaagccc gtgacgggct tttcttgtat tatgggtagt ttccttgcat gaatccataa 240
aaggcgcctg tagtgccatt tacccccatt cactgccaga gccgtgagcg cagcgaactg 300
aatgtcacga aaaagacagc gactcaggtg cctgatggtc ggagacaaaa ggaatattca 360
gcgatttgcc cgagcttgcg agggtgctac ttaagccttt agggttttaa ggtctgtttt 420
gtagaggagc aaacagcgtt tgcgacatcc ttttgtaata ctgcggaact gactaaagta 480
gtgagttata cacagggctg ggatctattc tttttatctt tttttattct ttctttattc 540
tataaattat aaccacttga atataaacaa aaaaaacaca caaaggtcta gcggaattta 600
cagagggtct agcagaattt acaagttttc cagcaaaggt ctagcagaat ttacagatac 660
ccacaactca aaggaaaagg actagtaatt atcattgact agcccatctc aattggtata 720
gtgattaaaa tcacctagac caattgagat gtatgtctga attagttgtt ttcaaagcaa 780
atgaactagc gattagtcgc tatgacttaa cggagcatga aaccaagcta attttatgct 840
gtgtggcact actcaacccc acgattgaaa accctacaag gaaagaacgg acggtatcgt 900
tcacttataa ccaatacgct cagatgatga acatcagtag ggaaaatgct tatggtgtat 960
tagctaaagc aaccagagag ctgatgacga gaactgtgga aatcaggaat cctttggtta 1020
aaggctttga gattttccag tggacaaact atgccaagtt ctcaagcgaa aaattagaat 1080
tagtttttag tgaagagata ttgccttatc ttttccagtt aaaaaaattc ataaaatata 1140
atctggaaca tgttaagtct tttgaaaaca aatactctat gaggatttat gagtggttat 1200
taaaagaact aacacaaaag aaaactcaca aggcaaatat agagattagc cttgatgaat 1260
ttaagttcat gttaatgctt gaaaataact accatgagtt taaaaggctt aaccaatggg 1320
ttttgaaacc aataagtaaa gatttaaaca cttacagcaa tatgaaattg gtggttgata 1380
agcgaggccg cccgactgat acgttgattt tccaagttga actagataga caaatggatc 1440
tcgtaaccga acttgagaac aaccagataa aaatgaatgg tgacaaaata ccaacaacca 1500
ttacatcaga ttcctaccta cgtaacggac taagaaaaac actacacgat gctttaactg 1560
caaaaattca gctcaccagt tttgaggcaa aatttttgag tgacatgcaa agtaagcatg 1620
atctcaatgg ttcgttctca tggctcacgc aaaaacaacg aaccacacta gagaacatac 1680
tggctaaata cggaaggatc tgaggttctt atggctcttg tatctatcag tgaagcatca 1740
agactaacaa acaaaagtag aacaactgtt caccgttaga tatcaaaggg aaaactgtcc 1800
atatgcacag atgaaaacgg tgtaaaaaag atagatacat cagagctttt acgagttttt 1860
ggtgcattta aagctgttca ccatgaacag atcgacaatg taacagatga acagcatgta 1920
acacctaata gaacaggtga aaccagtaaa acaaagcaac tagaacatga aattgaacac 1980
ctgagacaac ttgttacagc tcaacagtca cacatagaca gcctgaaaca ggcgatgctg 2040
cttatcgaat caaagctgcc gacaacacgg gagccagtga cgcctcccgt ggggaaaaaa 2100
tcatggcaat tctggaagaa atagcgcttt cagccggcaa acctgaagcc ggatctgcga 2160
ttctgataac aaactagcaa caccagaaca gcccgtttgc gggcagcaaa acccgttatg 2220
cttgtaaacc gttttgtgaa aaaattttta aaataaaaaa ggggacctct agggtcccca 2280
attaattagt aatataatct attaaaggtc attcaaaagg tcatccaccg gatcaattcc 2340
cctgctcgcg caggctgggt gccaagctct cgggtaacat caaggcccga tccttggagc 2400
ccttgccctc ccgcacgatg atcgtgccgt gatcgaaatc cagatccttg acccgcagtt 2460
gcaaaccctc actgatccgc atgcccgttc catacagaag ctgggcgaac aaacgatgct 2520
cgccttccag aaaaccgagg atgcgaacca cttcatccgg ggtcagcacc accggcaagc 2580
gccgcgacgg ccgaggtctt ccgatctcct gaagccaggg cagatccgtg cacagcacct 2640
tgccgtagaa gaacagcaag gccgccaatg cctgacgatg cgtggagacc gaaaccttgc 2700
gctcgttcgc cagccaggac agaaatgcct cgacttcgct gctgcccaag gttgccgggt 2760
gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc 2820
gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac 2880
gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg 2940
tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga 3000
tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca 3060
tcatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca 3120
tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg 3180
gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg 3240
aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga 3300
gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc 3360
gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag 3420
gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag 3480
aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac 3540
aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg 3600
ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg 3660
gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt 3720
atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg 3780
cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg 3840
tagtcggcaa ataatgtcta acaattcgtt caagccgacg ccgcttcgcg gcgcggctta 3900
actcaagcgt tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc 3960
tgcttttatt atttttaagc gtgcataata agccctacac aaattgggag atatatcatg 4020
aaaggctggc tttttcttgt tatcgcaata gttggcgaag taatcgcaac atccgcatta 4080
aaatctagcg agggctttac taagctgatc cggtggatga ccttttgaat gacctttaat 4140
agattatatt actaattaat tggggaccct agaggtcccc ttttttattt taaaaatttt 4200
ttcacaaaac ggtttacaag catacgttgg ccgattcatt aatgcagctg gcacgacagg 4260
tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat 4320
taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc 4380
ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct tgcatgcctg 4440
caggtcgact ctagaggatc ccccccgccg ccgacagagt aataggtttt acttaatagc 4500
tcttcctgtc ccttccaggc agtgatccgc attccgttct catggcgagg caacatttcg 4560
ggatggaaga taatgttctt tgctacagga aaatcaacaa tatgcgcacc agatgccact 4620
ggcagccgcc cgctgcgcgt tactaactct ataaatgcag ggatctcatc aatgacaaca 4680
tcctgcggac tgtttcctgc cagtcccatg atgatggcga catccgtggc atggcctttg 4740
cccgtcagtg acaacgaccc gtacagatcg accacaatat ggctcgtcgc ggttaataag 4800
ccgctacttt ccagccgatc aataaaactt tttccggcat tcattggccc cacggtatgc 4860
gaactggagg gaccaatccc aattttgaaa atatcgaatg cactaatcat atccacaccc 4920
tcggattgcc gttcagtgaa gtggagcgga acgaccttac gaccgtcccg ctcacgaggc 4980
tttacgcact acgtactgcg atggcttcaa tttccagcgg gagggcggat ccactaatac 5040
aaaatatatc aaaagttaat aataatatta ttcttactta agactttttt gtcttcattt 5100
tttagtaaaa aatataaaaa aggccacctc ccgattttat cggaaggcag cctcttaaat 5160
tcagttcata atattaaaaa atattattca acttcagaat atttgttggc ataggcagct 5220
gccgcaccca acagtccagg ctgcggataa gtaatcaact taaccggaat cttggacatg 5280
acgcgttcaa agcgtccttt tgaaacaaag cgctgacgga aaccagattc tggcaaatgg 5340
gaagcgatac gaagaccgac accaccgcca ataacaacac tggttgcacc ctgtgccaaa 5400
gcaagatcac cagcgatagc gccaaggctc aagcagaagc gatccaaagc ggcttcagca 5460
aggttgtctt taccttccaa agccatctgc cataatttaa tatcatccag caagctgaac 5520
ggaacgcctt caatggcagc cagtgcttcg tagatattac caagacccgg gccagaaata 5580
atgcgttcga tagaaacgcg gcggaaacgt tcacgtaaac gtgccagaat tttgtcttca 5640
agtctgtcaa gcggagcaaa gtcgatatga ccgccttcag tttcgatgac gaaataacgg 5700
ccttcagtcc gcaacagatg ggcaacaccc aagcccgttc ccggaccaag aatagtgata 5760
acaccatcgc taggaagcgc ttcatcagga ccacaaatat gatccagata agaagaatcc 5820
atatgcgcaa ccgcgtgggc aaccgcgccg aagtcattga tcagaacatg cgtatcgatg 5880
tccagctttt cattcagagt agctggtctt aatacccaag ggttattggt aagttttaaa 5940
acttcaccat gaaccgggcc agcccatgca atagctgcgg cacgtggcag aggacgaccc 6000
agtttttcac cgaaacgttc ccaagctaac tgcaagctag catgttctgc cgttttaaaa 6060
gttgtttctt ctccaagaga aagaacccga ccattgctta cttccgcaat agagaaacgc 6120
gcatgcgttc caccgatgtc aatcgcaaca atttccataa taattccttt ctgaaatcag 6180
aaggctaccc aacaggtaaa ataagtccgc ccgctttata ccatcgttgt aaacaaaaag 6240
tataattggt taagacttat ctaaaaaaga caaaaggatt cagccaaagc aagtttaact 6300
acttctggga gcgccacatc tcctcgattt catccaggct ccgacctttg gtttccggca 6360
cgaagcgagc aacaatcaag ccacctaaga tacttaatgc tgcgaaaacg agataggaga 6420
aaccgtggtt gaaagtctga ttcaatgctg gagaaccatc ggcaacctta aacaggaagt 6480
taaccaagat attagctaac cattgtccgg taacagcgat aggcatagct gcgcccttga 6540
tggaactcgg gaacatttct gacagaacaa cccagcagac agggccccat gacataccaa 6600
agactgcaat ataaagaagc acagaagcca aaggcaaaac accaccgact ttgaaccaga 6660
aacagcagcc taaaacagcc atcattgcag ccataccgag agcaccccaa ataagcagag 6720
gtttacggcc gaagcggtca acaacacggg aagcaatcat ggtgaagatg aagttcacaa 6780
caccgataga gatggtctgc aataatgccg tatcagctcc aaaacctaaa ttctggaaca 6840
tctgcggtgc ataatacagc acggcgttaa taccgactaa ctgctggaag gcagcaacgg 6900
atacaccggc aaaaacaacg gtgataccaa aagcaaacaa acctgcgctg cttttgtcca 6960
tggctttatc aaagccagct ttaatctttt gaatcgtcag attaggatcg gcttgcggtt 7020
ccagacgagc aaggattttg ctagcctcgg aatgacgtcc cttcatcacc aaccaatgcg 7080
gcgtatccgg tgcggttaac agcagcaata agaaggcaat accgatcagg ccttctgaag 7140
ccggagacca gcaccaacca ctggcattaa cccaatcgat agaaccgaaa tgagccagta 7200
accaggtaaa gatataaccg gttaaagcac ccgtcacaat ggccatctgc tgaccagaaa 7260
ccatctgacc acgtttgtct ggcggagcaa tttcagcaat ataggttggg gtcaaggttg 7320
aaacgacacc gatacctaaa ccggcaagaa accggaaaaa gcaaaaaatt tgtaaagccg 7380
aaccaccggt tccaaataat ttttcggtta acgcagcacc aaaaccggcg gcgacgaaac 7440
aaatggaact catcaacaat ccgccgcgac gaccgaagcg aataccaatc cagccagaca 7500
gcaaagaacc ggtaacacaa ccgaccaaaa cagcaacaac gaccatccca gaaagggaag 7560
ccgcagccgt agcagacagg tgacgagggg caataaaatg gatatcaacc ggtgtaccga 7620
ttgcagcgat aaccgctgaa tcgtaaccga aaagcaagcc gcctatagca gcgattaggg 7680
ctagtcgcgt gactagaccc tgactacttt cagaactcat ggcgattcct ctccctctag 7740
agcgtcctgc tgttgttaag attattatac cacaccttgt agataaagtc aacaactttt 7800
tgcaaaattt ttcaggaatt ttagcagagg ttgttctgga tgtagaacaa aacatctttc 7860
cgctcttgtg ctgttaggat atctttcttg gaagctaggt aggcctcgag ttatggcagt 7920
tggttaaaag gaaacaaaaa gaccgttttc acacaaaacg gtctttttcg atttcttttt 7980
acagtcacag ccacttttgc accaattaag gccacgctgt catttaaact ccgtttttcc 8040
agttcaaatg caattgcctt caatgcacct tcgtagctgt ggtgagccag cggtgctggc 8100
tctcccccat ttacggataa gaatgcattt tccgagttaa taccgtcggc aatacctgac 8160
attaatactt cacagtcgct ggcatcgagt acggaaaact taatcgaaga cgaaccacag 8220
ttaataacca aaacaaccgg aaattcattc atctcttttc tcatcctgag ttacggatta 8280
aaacagtttg tatacgatgt tcaggatggt cagcagacca atcacggtaa caaacacgtt 8340
atccagacga ccacggtatt tcgccagaga cggcgcttta cggatggcat acatcggcaa 8400
caggcacagc agggatgcga taatcggtgc gcccatggct tcaatcaggt cgaggatgtt 8460
cgggttggcg taggcaacaa cccaggtgga gcccatgatg aagatcatgc tgagagtatt 8520
cagtttaccc agcgacactt tggttttgtc acctttataa ccgaacttca gaatcagacc 8580
attcaagcct tccagcgtcc ccagatagtg accgaagaaa gatttgaaga tagccacgag 8640
tgcgatgatg gaagccgcat attccagtgt aatcgcgaac gttgttttgg taccggtcat 8700
ggacgcaaag tggttagcca gataagaaag cactggaata ttctgcgctt tggcttccgc 8760
catgttggcc ggagacagag taaacaggca gctaaaggca aagaacatca ccactgcaac 8820
catcagcatg ctggcacgag aaatgatttg ggaacattta cgttcggtga agtcgcgacc 8880
gaagtctttc tcatactctt cacgtttaga aaccacgaag gaagagacga ttggcgagaa 8940
gttaaaggag aaaaccatga tggaaatccc cagccagaca gtgatcagga taccgtcatg 9000
accggttaac gacagcgaac cgaggtcaac ctggtcgata actgcagagt tccagtaagg 9060
gatcagcgac aaagaaatca gcaccaggct ggcgataaac ggccatacca ggtagctcag 9120
ggtaccgagc tcgaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg 9180
cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga 9240
agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct 9300
gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct 9360
cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc 9420
tgacgaattc 9430
<210> 17
<211> 5768
<212> DNA
<213> 质粒pMH17F;
<400> 17
gttgacagta agacgggtaa gcctgttgat gataccgctg ccttactggg tgcattagcc 60
agtctgaatg acctgtcacg ggataatccg aagtggtcag actggaaaat cagagggcag 120
gaactgctga acagcaaaaa gtcagatagc accacatagc agacccgcca taaaacgccc 180
tgagaagccc gtgacgggct tttcttgtat tatgggtagt ttccttgcat gaatccataa 240
aaggcgcctg tagtgccatt tacccccatt cactgccaga gccgtgagcg cagcgaactg 300
aatgtcacga aaaagacagc gactcaggtg cctgatggtc ggagacaaaa ggaatattca 360
gcgatttgcc cgagcttgcg agggtgctac ttaagccttt agggttttaa ggtctgtttt 420
gtagaggagc aaacagcgtt tgcgacatcc ttttgtaata ctgcggaact gactaaagta 480
gtgagttata cacagggctg ggatctattc tttttatctt tttttattct ttctttattc 540
tataaattat aaccacttga atataaacaa aaaaaacaca caaaggtcta gcggaattta 600
cagagggtct agcagaattt acaagttttc cagcaaaggt ctagcagaat ttacagatac 660
ccacaactca aaggaaaagg actagtaatt atcattgact agcccatctc aattggtata 720
gtgattaaaa tcacctagac caattgagat gtatgtctga attagttgtt ttcaaagcaa 780
atgaactagc gattagtcgc tatgacttaa cggagcatga aaccaagcta attttatgct 840
gtgtggcact actcaacccc acgattgaaa accctacaag gaaagaacgg acggtatcgt 900
tcacttataa ccaatacgct cagatgatga acatcagtag ggaaaatgct tatggtgtat 960
tagctaaagc aaccagagag ctgatgacga gaactgtgga aatcaggaat cctttggtta 1020
aaggctttga gattttccag tggacaaact atgccaagtt ctcaagcgaa aaattagaat 1080
tagtttttag tgaagagata ttgccttatc ttttccagtt aaaaaaattc ataaaatata 1140
atctggaaca tgttaagtct tttgaaaaca aatactctat gaggatttat gagtggttat 1200
taaaagaact aacacaaaag aaaactcaca aggcaaatat agagattagc cttgatgaat 1260
ttaagttcat gttaatgctt gaaaataact accatgagtt taaaaggctt aaccaatggg 1320
ttttgaaacc aataagtaaa gatttaaaca cttacagcaa tatgaaattg gtggttgata 1380
agcgaggccg cccgactgat acgttgattt tccaagttga actagataga caaatggatc 1440
tcgtaaccga acttgagaac aaccagataa aaatgaatgg tgacaaaata ccaacaacca 1500
ttacatcaga ttcctaccta cgtaacggac taagaaaaac actacacgat gctttaactg 1560
caaaaattca gctcaccagt tttgaggcaa aatttttgag tgacatgcaa agtaagcatg 1620
atctcaatgg ttcgttctca tggctcacgc aaaaacaacg aaccacacta gagaacatac 1680
tggctaaata cggaaggatc tgaggttctt atggctcttg tatctatcag tgaagcatca 1740
agactaacaa acaaaagtag aacaactgtt caccgttaga tatcaaaggg aaaactgtcc 1800
atatgcacag atgaaaacgg tgtaaaaaag atagatacat cagagctttt acgagttttt 1860
ggtgcattta aagctgttca ccatgaacag atcgacaatg taacagatga acagcatgta 1920
acacctaata gaacaggtga aaccagtaaa acaaagcaac tagaacatga aattgaacac 1980
ctgagacaac ttgttacagc tcaacagtca cacatagaca gcctgaaaca ggcgatgctg 2040
cttatcgaat caaagctgcc gacaacacgg gagccagtga cgcctcccgt ggggaaaaaa 2100
tcatggcaat tctggaagaa atagcgcttt cagccggcaa acctgaagcc ggatctgcga 2160
ttctgataac aaactagcaa caccagaaca gcccgtttgc gggcagcaaa acccgttatg 2220
cttgtaaacc gttttgtgaa aaaattttta aaataaaaaa ggggacctct agggtcccca 2280
attaattagt aatataatct attaaaggtc attcaaaagg tcatccaccg gatcaattcc 2340
cctgctcgcg caggctgggt gccaagctct cgggtaacat caaggcccga tccttggagc 2400
ccttgccctc ccgcacgatg atcgtgccgt gatcgaaatc cagatccttg acccgcagtt 2460
gcaaaccctc actgatccgc atgcccgttc catacagaag ctgggcgaac aaacgatgct 2520
cgccttccag aaaaccgagg atgcgaacca cttcatccgg ggtcagcacc accggcaagc 2580
gccgcgacgg ccgaggtctt ccgatctcct gaagccaggg cagatccgtg cacagcacct 2640
tgccgtagaa gaacagcaag gccgccaatg cctgacgatg cgtggagacc gaaaccttgc 2700
gctcgttcgc cagccaggac agaaatgcct cgacttcgct gctgcccaag gttgccgggt 2760
gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc 2820
gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac 2880
gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg 2940
tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga 3000
tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca 3060
tcatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca 3120
tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg 3180
gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg 3240
aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga 3300
gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc 3360
gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag 3420
gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag 3480
aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac 3540
aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg 3600
ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg 3660
gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt 3720
atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg 3780
cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg 3840
tagtcggcaa ataatgtcta acaattcgtt caagccgacg ccgcttcgcg gcgcggctta 3900
actcaagcgt tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc 3960
tgcttttatt atttttaagc gtgcataata agccctacac aaattgggag atatatcatg 4020
aaaggctggc tttttcttgt tatcgcaata gttggcgaag taatcgcaac atccgcatta 4080
aaatctagcg agggctttac taagctgatc cggtggatga ccttttgaat gacctttaat 4140
agattatatt actaattaat tggggaccct agaggtcccc ttttttattt taaaaatttt 4200
ttcacaaaac ggtttacaag catacgttgg ccgattcatt aatgcagctg gcacgacagg 4260
tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat 4320
taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagt 4380
tacctagaga gggtgagaat tgccgaacat gcgcataagt ttcccggaca gatttcaggt 4440
ggtcagcagc aacgcgttgc cattgcgcgt tcgctgtgta tgaagccgaa aattatgttg 4500
tttgatgagc caacgtcggc gctcgatcct gagatggtga aagaggtgct ggatacgatg 4560
attgggctgg cgcagtcggg tatgacaatg ttgtgtgtaa cacatgagat ggggtttgca 4620
cgaaccgtcg ctgaccgggt aatttttatg gatcgtgggg aaatagtgga gcaagctgca 4680
cctgatgaat tttttgcgca tcctaaatca gagcgtacga gggcattttt atcgcaggta 4740
atccattaat tgaatgttag ttcgaaaagc aaaaaggcca tcctttcgga tggcctttcg 4800
cttgatttga tgtctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg 4860
cttcgcaacg ttcaaatccg ctcccggcgg atttgtccta ctcgggagag tgttcaccga 4920
caaacaacag ataaaacaaa aggcccagtc ttccgactga gccttttgtt ttatttgatg 4980
tctggcagtt ccctactctc gcatggggag accccacact accatcggcg ctacggcggt 5040
ttcacttctg agttcggcat ggggtcaggt gggaccaccg cgctactgcc gccagacaaa 5100
ttcttttcta atctgccgaa ctttaaccta aaaagtggtg ctgataccca gagtcgaact 5160
ggggacctca cccttaccaa gggtgcgctc taccaactga gccatatcag cacgctaaat 5220
ttgatgcctg gcagttccct actctcgcat ggggagaccc cacactacca tcggcgctac 5280
ggcgtttcac ttctgagttc ggcatggggt caggtgggac caccgcgcta cggccgccag 5340
gcaaattctg ttttatcaga ccgcttctgc gttctgattt aatctgtatc aggctgaaaa 5400
tcttctctca tccggataac aatttcacac aggaaacagc tatgaccatg attacgccaa 5460
gctcgagctc gaattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg 5520
ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag 5580
aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga 5640
tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca 5700
gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg 5760
acgaattc 5768
<210> 18
<211> 1053
<212> DNA
<213> 野生型aroG基因的编码区;
<400> 18
atgaattatc agaacgacga tttacgcatc aaagaaatca aagagttact tcctcctgtc 60
gcattgctgg aaaaattccc cgctactgaa aatgccgcga atacggttgc ccatgcccga 120
aaagcgatcc ataagatcct gaaaggtaat gatgatcgcc tgttggttgt gattggccca 180
tgctcaattc atgatcctgt cgcggcaaaa gagtatgcca ctcgcttgct ggcgctgcgt 240
gaagagctga aagatgagct ggaaatcgta atgcgcgtct attttgaaaa gccgcgtacc 300
acggtgggct ggaaagggct gattaacgat ccgcatatgg ataatagctt ccagatcaac 360
gacggtctgc gtatagcccg taaattgctg cttgatatta acgacagcgg tctgccagcg 420
gcaggtgagt ttctcgatat gatcacccca caatatctcg ctgacctgat gagctggggc 480
gcaattggcg cacgtaccac cgaatcgcag gtgcaccgcg aactggcatc agggctttct 540
tgtccggtcg gcttcaaaaa tggcaccgac ggtacgatta aagtggctat cgatgccatt 600
aatgccgccg gtgcgccgca ctgcttcctg tccgtaacga aatgggggca ttcggcgatt 660
gtgaatacca gcggtaacgg cgattgccat atcattctgc gcggcggtaa agagcctaac 720
tacagcgcga agcacgttgc tgaagtgaaa gaagggctga acaaagcagg cctgccagca 780
caggtgatga tcgatttcag ccatgctaac tcgtccaaac aattcaaaaa gcagatggat 840
gtttgtgctg acgtttgcca gcagattgcc ggtggcgaaa aggccattat tggcgtgatg 900
gtggaaagcc atctggtgga aggcaatcag agcctcgaga gcggggagcc gctggcctac 960
ggtaagagca tcaccgatgc ctgcatcggc tgggaagata ccgatgctct gttacgtcaa 1020
ctggcgaatg cagtaaaagc gcgtcgcggg taa 1053
<210> 19
<211> 8820
<212> DNA
<213> 质粒pMH28F;
<220>
<221> misc_feature
<222> (5894)..(5894)
<223> n是a、c、g或t
<400> 19
gttgacagta agacgggtaa gcctgttgat gataccgctg ccttactggg tgcattagcc 60
agtctgaatg acctgtcacg ggataatccg aagtggtcag actggaaaat cagagggcag 120
gaactgctga acagcaaaaa gtcagatagc accacatagc agacccgcca taaaacgccc 180
tgagaagccc gtgacgggct tttcttgtat tatgggtagt ttccttgcat gaatccataa 240
aaggcgcctg tagtgccatt tacccccatt cactgccaga gccgtgagcg cagcgaactg 300
aatgtcacga aaaagacagc gactcaggtg cctgatggtc ggagacaaaa ggaatattca 360
gcgatttgcc cgagcttgcg agggtgctac ttaagccttt agggttttaa ggtctgtttt 420
gtagaggagc aaacagcgtt tgcgacatcc ttttgtaata ctgcggaact gactaaagta 480
gtgagttata cacagggctg ggatctattc tttttatctt tttttattct ttctttattc 540
tataaattat aaccacttga atataaacaa aaaaaacaca caaaggtcta gcggaattta 600
cagagggtct agcagaattt acaagttttc cagcaaaggt ctagcagaat ttacagatac 660
ccacaactca aaggaaaagg actagtaatt atcattgact agcccatctc aattggtata 720
gtgattaaaa tcacctagac caattgagat gtatgtctga attagttgtt ttcaaagcaa 780
atgaactagc gattagtcgc tatgacttaa cggagcatga aaccaagcta attttatgct 840
gtgtggcact actcaacccc acgattgaaa accctacaag gaaagaacgg acggtatcgt 900
tcacttataa ccaatacgct cagatgatga acatcagtag ggaaaatgct tatggtgtat 960
tagctaaagc aaccagagag ctgatgacga gaactgtgga aatcaggaat cctttggtta 1020
aaggctttga gattttccag tggacaaact atgccaagtt ctcaagcgaa aaattagaat 1080
tagtttttag tgaagagata ttgccttatc ttttccagtt aaaaaaattc ataaaatata 1140
atctggaaca tgttaagtct tttgaaaaca aatactctat gaggatttat gagtggttat 1200
taaaagaact aacacaaaag aaaactcaca aggcaaatat agagattagc cttgatgaat 1260
ttaagttcat gttaatgctt gaaaataact accatgagtt taaaaggctt aaccaatggg 1320
ttttgaaacc aataagtaaa gatttaaaca cttacagcaa tatgaaattg gtggttgata 1380
agcgaggccg cccgactgat acgttgattt tccaagttga actagataga caaatggatc 1440
tcgtaaccga acttgagaac aaccagataa aaatgaatgg tgacaaaata ccaacaacca 1500
ttacatcaga ttcctaccta cgtaacggac taagaaaaac actacacgat gctttaactg 1560
caaaaattca gctcaccagt tttgaggcaa aatttttgag tgacatgcaa agtaagcatg 1620
atctcaatgg ttcgttctca tggctcacgc aaaaacaacg aaccacacta gagaacatac 1680
tggctaaata cggaaggatc tgaggttctt atggctcttg tatctatcag tgaagcatca 1740
agactaacaa acaaaagtag aacaactgtt caccgttaga tatcaaaggg aaaactgtcc 1800
atatgcacag atgaaaacgg tgtaaaaaag atagatacat cagagctttt acgagttttt 1860
ggtgcattta aagctgttca ccatgaacag atcgacaatg taacagatga acagcatgta 1920
acacctaata gaacaggtga aaccagtaaa acaaagcaac tagaacatga aattgaacac 1980
ctgagacaac ttgttacagc tcaacagtca cacatagaca gcctgaaaca ggcgatgctg 2040
cttatcgaat caaagctgcc gacaacacgg gagccagtga cgcctcccgt ggggaaaaaa 2100
tcatggcaat tctggaagaa atagcgcttt cagccggcaa acctgaagcc ggatctgcga 2160
ttctgataac aaactagcaa caccagaaca gcccgtttgc gggcagcaaa acccgttatg 2220
cttgtaaacc gttttgtgaa aaaattttta aaataaaaaa ggggacctct agggtcccca 2280
attaattagt aatataatct attaaaggtc attcaaaagg tcatccaccg gatcaattcc 2340
cctgctcgcg caggctgggt gccaagctct cgggtaacat caaggcccga tccttggagc 2400
ccttgccctc ccgcacgatg atcgtgccgt gatcgaaatc cagatccttg acccgcagtt 2460
gcaaaccctc actgatccgc atgcccgttc catacagaag ctgggcgaac aaacgatgct 2520
cgccttccag aaaaccgagg atgcgaacca cttcatccgg ggtcagcacc accggcaagc 2580
gccgcgacgg ccgaggtctt ccgatctcct gaagccaggg cagatccgtg cacagcacct 2640
tgccgtagaa gaacagcaag gccgccaatg cctgacgatg cgtggagacc gaaaccttgc 2700
gctcgttcgc cagccaggac agaaatgcct cgacttcgct gctgcccaag gttgccgggt 2760
gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc 2820
gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac 2880
gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg 2940
tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga 3000
tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca 3060
tcatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca 3120
tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg 3180
gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg 3240
aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga 3300
gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc 3360
gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag 3420
gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag 3480
aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac 3540
aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg 3600
ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg 3660
gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt 3720
atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg 3780
cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg 3840
tagtcggcaa ataatgtcta acaattcgtt caagccgacg ccgcttcgcg gcgcggctta 3900
actcaagcgt tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc 3960
tgcttttatt atttttaagc gtgcataata agccctacac aaattgggag atatatcatg 4020
aaaggctggc tttttcttgt tatcgcaata gttggcgaag taatcgcaac atccgcatta 4080
aaatctagcg agggctttac taagctgatc cggtggatga ccttttgaat gacctttaat 4140
agattatatt actaattaat tggggaccct agaggtcccc ttttttattt taaaaatttt 4200
ttcacaaaac ggtttacaag catacgttgg ccgattcatt aatgcagctg gcacgacagg 4260
tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat 4320
taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagt 4380
tacctagaga gggtgagaat tgccgaacat gcgcataagt ttcccggaca gatttcaggt 4440
ggtcagcagc aacgcgttgc cattgcgcgt tcgctgtgta tgaagccgaa aattatgttg 4500
tttgatgagc caacgtcggc gctcgatcct gagatggtga aagaggtgct ggatacgatg 4560
attgggctgg cgcagtcggg tatgacaatg ttgtgtgtaa cacatgagat ggggtttgca 4620
cgaaccgtcg ctgaccgggt aatttttatg gatcgtgggg aaatagtgga gcaagctgca 4680
cctgatgaat tttttgcgca tcctaaatca gagcgtacga gggcattttt atcgcaggta 4740
atccattaat tgaatgttag ttcgaaaagc aaaaaggcca tcctttcgga tggcctttcg 4800
cttgatttga tgtctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg 4860
cttcgcaacg ttcaaatccg gtgacggaag atcacttcgc agaataaata aatcctggtg 4920
tccctgttga taccgggaag ccctgggcca acttttggcg aaaatgagac gttgatcggc 4980
acgtaagagg ttccaacttt caccataatg aaataagatc actaccgggc gtattttttg 5040
agttatcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata 5100
ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg 5160
ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa 5220
agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg 5280
ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc 5340
acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat 5400
accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg 5460
aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc 5520
cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc 5580
ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga 5640
ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac 5700
aacagtactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt tattggtgcc 5760
cttaaacgcc tggtgctacg cctgaataag tgataataag cggatgaatg gcagaaattc 5820
gaaagcaaat tcgacccggt cgtcggttca gggcagggtc gttaaatagc cgcttatgtc 5880
tattgctggt ttantcggta cccggggatc gcggccgcgg accggatccc atcacatata 5940
cctgccgttc actattattt agtgaaatga gatattatga tattttctga attgtgatta 6000
aaaaggcaac tttatgccca tgcaacagaa actataaaaa atacagagaa tgaaaagaaa 6060
cagatagatt ttttagttct ttaggcccgt agtctgcaaa tccttttatg attttctatc 6120
aaacaaaaga ggaaaataga ccagttgcaa tccaaacgag agtctaatag aatgaggtcg 6180
aaaagtaaat cgcgcgggtt tgttactgat aaagcaggca agacctaaaa tgtgtaaagg 6240
gcaaagtgta tactttggcg tcacccctta catattttag gtcttttttt attgtgcgta 6300
actaacttgc catcttcaaa caggagggct ggaagaagca gaccgctaac acagtacata 6360
aaaaaggaga catgaacgat gaacatcaaa aagtttgcaa aacaagcaac agtattaacc 6420
tttactaccg cactgctggc aggaggcgca actcaagcgt ttgcgaaaga aacgaaccaa 6480
aagccatata aggaaacata cggcatttcc catattacac gccatgatat gctgcaaatc 6540
cctgaacagc aaaaaaatga aaaatatcaa gttcctgaat tcgattcgtc cacaattaaa 6600
aatatctctt ctgcaaaagg cctggacgtt tgggacagct ggccattaca aaacgctgac 6660
ggcactgtcg caaactatca cggctaccac atcgtctttg cattagccgg agatcctaaa 6720
aatgcggatg acacatcgat ttacatgttc tatcaaaaag tcggcgaaac ttctattgac 6780
agctggaaaa acgctggccg cgtctttaaa gacagcgaca aattcgatgc aaatgattct 6840
atcctaaaag accaaacaca agaatggtca ggttcagcca catttacatc tgacggaaaa 6900
atccgtttat tctacactga tttctccggt aaacattacg gcaaacaaac actgacaact 6960
gcacaagtta acgtatcagc atcagacagc tctttgaaca tcaacggtgt agaggattat 7020
aaatcaatct ttgacggtga cggaaaaacg tatcaaaatg tacagcagtt catcgatgaa 7080
ggcaactaca gctcaggcga caaccatacg ctgagagatc ctcactacgt agaagataaa 7140
ggccacaaat acttagtatt tgaagcaaac actggaactg aagatggcta ccaaggcgaa 7200
gaatctttat ttaacaaagc atactatggc aaaagcacat cattcttccg tcaagaaagt 7260
caaaaacttc tgcaaagcga taaaaaacgc acggctgagt tagcaaacgg cgctctcggt 7320
atgattgagc taaacgatga ttacacactg aaaaaagtga tgaaaccgct gattgcatct 7380
aacacagtaa cagatgaaat tgaacgcgcg aacgtcttta aaatgaacgg caaatggtac 7440
ctgttcactg actcccgcgg atcaaaaatg acgattgacg gcattacgtc taacgatatt 7500
tacatgcttg gttatgtttc taattcttta actggcccat acaagccgct gaacaaaact 7560
ggccttgtgt taaaaatgga tcttgatcct aacgatgtaa cctttactta ctcacacttc 7620
gctgtacctc aagcgaaagg aaacaatgtc gtgattacaa gctatatgac aaacagagga 7680
ttctacgcag acaaacaatc aacgtttgcg ccgagcttcc tgctgaacat caaaggcaag 7740
aaaacatctg ttgtcaaaga cagcatcctt gaacaaggac aattaacagt taacaaataa 7800
aaacgcaaaa gaaaatgcca atatcctatt ggcattttct tttatttctt ccatttaaat 7860
ggatgcatgc gctagcggag tgtatactgg cttactatgt tggcactgat gagggtgtca 7920
gtgaagtgct tcctcccggc ggatttgtcc tactcgggag agtgttcacc gacaaacaac 7980
agataaaaca aaaggcccag tcttccgact gagccttttg ttttatttga tgtctggcag 8040
ttccctactc tcgcatgggg agaccccaca ctaccatcgg cgctacggcg gtttcacttc 8100
tgagttcggc atggggtcag gtgggaccac cgcgctactg ccgccagaca aattcttttc 8160
taatctgccg aactttaacc taaaaagtgg tgctgatacc cagagtcgaa ctggggacct 8220
cacccttacc aagggtgcgc tctaccaact gagccatatc agcacgctaa atttgatgcc 8280
tggcagttcc ctactctcgc atggggagac cccacactac catcggcgct acggcgtttc 8340
acttctgagt tcggcatggg gtcaggtggg accaccgcgc tacggccgcc aggcaaattc 8400
tgttttatca gaccgcttct gcgttctgat ttaatctgta tcaggctgaa aatcttctct 8460
catccggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagctcgagc 8520
tcgaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa 8580
cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc 8640
accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat 8700
tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc 8760
tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgaattc 8820
<210> 20
<211> 4774
<212> DNA
<213> 质粒pCL1921;
<400> 20
gttgacagta agacgggtaa gcctgttgat gataccgctg ccttactggg tgcattagcc 60
agtctgaatg acctgtcacg ggataatccg aagtggtcag actggaaaat cagagggcag 120
gaactgctga acagcaaaaa gtcagatagc accacatagc agacccgcca taaaacgccc 180
tgagaagccc gtgacgggct tttcttgtat tatgggtagt ttccttgcat gaatccataa 240
aaggcgcctg tagtgccatt tacccccatt cactgccaga gccgtgagcg cagcgaactg 300
aatgtcacga aaaagacagc gactcaggtg cctgatggtc ggagacaaaa ggaatattca 360
gcgatttgcc cgagcttgcg agggtgctac ttaagccttt agggttttaa ggtctgtttt 420
gtagaggagc aaacagcgtt tgcgacatcc ttttgtaata ctgcggaact gactaaagta 480
gtgagttata cacagggctg ggatctattc tttttatctt tttttattct ttctttattc 540
tataaattat aaccacttga atataaacaa aaaaaacaca caaaggtcta gcggaattta 600
cagagggtct agcagaattt acaagttttc cagcaaaggt ctagcagaat ttacagatac 660
ccacaactca aaggaaaagg actagtaatt atcattgact agcccatctc aattggtata 720
gtgattaaaa tcacctagac caattgagat gtatgtctga attagttgtt ttcaaagcaa 780
atgaactagc gattagtcgc tatgacttaa cggagcatga aaccaagcta attttatgct 840
gtgtggcact actcaacccc acgattgaaa accctacaag gaaagaacgg acggtatcgt 900
tcacttataa ccaatacgct cagatgatga acatcagtag ggaaaatgct tatggtgtat 960
tagctaaagc aaccagagag ctgatgacga gaactgtgga aatcaggaat cctttggtta 1020
aaggctttga gattttccag tggacaaact atgccaagtt ctcaagcgaa aaattagaat 1080
tagtttttag tgaagagata ttgccttatc ttttccagtt aaaaaaattc ataaaatata 1140
atctggaaca tgttaagtct tttgaaaaca aatactctat gaggatttat gagtggttat 1200
taaaagaact aacacaaaag aaaactcaca aggcaaatat agagattagc cttgatgaat 1260
ttaagttcat gttaatgctt gaaaataact accatgagtt taaaaggctt aaccaatggg 1320
ttttgaaacc aataagtaaa gatttaaaca cttacagcaa tatgaaattg gtggttgata 1380
agcgaggccg cccgactgat acgttgattt tccaagttga actagataga caaatggatc 1440
tcgtaaccga acttgagaac aaccagataa aaatgaatgg tgacaaaata ccaacaacca 1500
ttacatcaga ttcctaccta cgtaacggac taagaaaaac actacacgat gctttaactg 1560
caaaaattca gctcaccagt tttgaggcaa aatttttgag tgacatgcaa agtaagcatg 1620
atctcaatgg ttcgttctca tggctcacgc aaaaacaacg aaccacacta gagaacatac 1680
tggctaaata cggaaggatc tgaggttctt atggctcttg tatctatcag tgaagcatca 1740
agactaacaa acaaaagtag aacaactgtt caccgttaga tatcaaaggg aaaactgtcc 1800
atatgcacag atgaaaacgg tgtaaaaaag atagatacat cagagctttt acgagttttt 1860
ggtgcattta aagctgttca ccatgaacag atcgacaatg taacagatga acagcatgta 1920
acacctaata gaacaggtga aaccagtaaa acaaagcaac tagaacatga aattgaacac 1980
ctgagacaac ttgttacagc tcaacagtca cacatagaca gcctgaaaca ggcgatgctg 2040
cttatcgaat caaagctgcc gacaacacgg gagccagtga cgcctcccgt ggggaaaaaa 2100
tcatggcaat tctggaagaa atagcgcttt cagccggcaa acctgaagcc ggatctgcga 2160
ttctgataac aaactagcaa caccagaaca gcccgtttgc gggcagcaaa acccgttatg 2220
cttgtaaacc gttttgtgaa aaaattttta aaataaaaaa ggggacctct agggtcccca 2280
attaattagt aatataatct attaaaggtc attcaaaagg tcatccaccg gatcaattcc 2340
cctgctcgcg caggctgggt gccaagctct cgggtaacat caaggcccga tccttggagc 2400
ccttgccctc ccgcacgatg atcgtgccgt gatcgaaatc cagatccttg acccgcagtt 2460
gcaaaccctc actgatccgc atgcccgttc catacagaag ctgggcgaac aaacgatgct 2520
cgccttccag aaaaccgagg atgcgaacca cttcatccgg ggtcagcacc accggcaagc 2580
gccgcgacgg ccgaggtctt ccgatctcct gaagccaggg cagatccgtg cacagcacct 2640
tgccgtagaa gaacagcaag gccgccaatg cctgacgatg cgtggagacc gaaaccttgc 2700
gctcgttcgc cagccaggac agaaatgcct cgacttcgct gctgcccaag gttgccgggt 2760
gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc 2820
gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac 2880
gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg 2940
tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga 3000
tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca 3060
tcatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca 3120
tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg 3180
gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg 3240
aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga 3300
gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc 3360
gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag 3420
gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag 3480
aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac 3540
aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg 3600
ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg 3660
gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt 3720
atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg 3780
cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg 3840
tagtcggcaa ataatgtcta acaattcgtt caagccgacg ccgcttcgcg gcgcggctta 3900
actcaagcgt tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc 3960
tgcttttatt atttttaagc gtgcataata agccctacac aaattgggag atatatcatg 4020
aaaggctggc tttttcttgt tatcgcaata gttggcgaag taatcgcaac atccgcatta 4080
aaatctagcg agggctttac taagctgatc cggtggatga ccttttgaat gacctttaat 4140
agattatatt actaattaat tggggaccct agaggtcccc ttttttattt taaaaatttt 4200
ttcacaaaac ggtttacaag catacgttgg ccgattcatt aatgcagctg gcacgacagg 4260
tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat 4320
taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc 4380
ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct tgcatgcctg 4440
caggtcgact ctagaggatc cccgggtacc gagctcgaat tcactggccg tcgttttaca 4500
acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc 4560
tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg 4620
cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat 4680
ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca 4740
gccccgacac ccgccaacac ccgctgacga attc 4774
<210> 21
<211> 6432
<212> DNA
<213> 质粒pMG27;
<400> 21
gttgacagta agacgggtaa gcctgttgat gataccgctg ccttactggg tgcattagcc 60
agtctgaatg acctgtcacg ggataatccg aagtggtcag actggaaaat cagagggcag 120
gaactgctga acagcaaaaa gtcagatagc accacatagc agacccgcca taaaacgccc 180
tgagaagccc gtgacgggct tttcttgtat tatgggtagt ttccttgcat gaatccataa 240
aaggcgcctg tagtgccatt tacccccatt cactgccaga gccgtgagcg cagcgaactg 300
aatgtcacga aaaagacagc gactcaggtg cctgatggtc ggagacaaaa ggaatattca 360
gcgatttgcc cgagcttgcg agggtgctac ttaagccttt agggttttaa ggtctgtttt 420
gtagaggagc aaacagcgtt tgcgacatcc ttttgtaata ctgcggaact gactaaagta 480
gtgagttata cacagggctg ggatctattc tttttatctt tttttattct ttctttattc 540
tataaattat aaccacttga atataaacaa aaaaaacaca caaaggtcta gcggaattta 600
cagagggtct agcagaattt acaagttttc cagcaaaggt ctagcagaat ttacagatac 660
ccacaactca aaggaaaagg actagtaatt atcattgact agcccatctc aattggtata 720
gtgattaaaa tcacctagac caattgagat gtatgtctga attagttgtt ttcaaagcaa 780
atgaactagc gattagtcgc tatgacttaa cggagcatga aaccaagcta attttatgct 840
gtgtggcact actcaacccc acgattgaaa accctacaag gaaagaacgg acggtatcgt 900
tcacttataa ccaatacgct cagatgatga acatcagtag ggaaaatgct tatggtgtat 960
tagctaaagc aaccagagag ctgatgacga gaactgtgga aatcaggaat cctttggtta 1020
aaggctttga gattttccag tggacaaact atgccaagtt ctcaagcgaa aaattagaat 1080
tagtttttag tgaagagata ttgccttatc ttttccagtt aaaaaaattc ataaaatata 1140
atctggaaca tgttaagtct tttgaaaaca aatactctat gaggatttat gagtggttat 1200
taaaagaact aacacaaaag aaaactcaca aggcaaatat agagattagc cttgatgaat 1260
ttaagttcat gttaatgctt gaaaataact accatgagtt taaaaggctt aaccaatggg 1320
ttttgaaacc aataagtaaa gatttaaaca cttacagcaa tatgaaattg gtggttgata 1380
agcgaggccg cccgactgat acgttgattt tccaagttga actagataga caaatggatc 1440
tcgtaaccga acttgagaac aaccagataa aaatgaatgg tgacaaaata ccaacaacca 1500
ttacatcaga ttcctaccta cgtaacggac taagaaaaac actacacgat gctttaactg 1560
caaaaattca gctcaccagt tttgaggcaa aatttttgag tgacatgcaa agtaagcatg 1620
atctcaatgg ttcgttctca tggctcacgc aaaaacaacg aaccacacta gagaacatac 1680
tggctaaata cggaaggatc tgaggttctt atggctcttg tatctatcag tgaagcatca 1740
agactaacaa acaaaagtag aacaactgtt caccgttaga tatcaaaggg aaaactgtcc 1800
atatgcacag atgaaaacgg tgtaaaaaag atagatacat cagagctttt acgagttttt 1860
ggtgcattta aagctgttca ccatgaacag atcgacaatg taacagatga acagcatgta 1920
acacctaata gaacaggtga aaccagtaaa acaaagcaac tagaacatga aattgaacac 1980
ctgagacaac ttgttacagc tcaacagtca cacatagaca gcctgaaaca ggcgatgctg 2040
cttatcgaat caaagctgcc gacaacacgg gagccagtga cgcctcccgt ggggaaaaaa 2100
tcatggcaat tctggaagaa atagcgcttt cagccggcaa acctgaagcc ggatctgcga 2160
ttctgataac aaactagcaa caccagaaca gcccgtttgc gggcagcaaa acccgttatg 2220
cttgtaaacc gttttgtgaa aaaattttta aaataaaaaa ggggacctct agggtcccca 2280
attaattagt aatataatct attaaaggtc attcaaaagg tcatccaccg gatcaattcc 2340
cctgctcgcg caggctgggt gccaagctct cgggtaacat caaggcccga tccttggagc 2400
ccttgccctc ccgcacgatg atcgtgccgt gatcgaaatc cagatccttg acccgcagtt 2460
gcaaaccctc actgatccgc atgcccgttc catacagaag ctgggcgaac aaacgatgct 2520
cgccttccag aaaaccgagg atgcgaacca cttcatccgg ggtcagcacc accggcaagc 2580
gccgcgacgg ccgaggtctt ccgatctcct gaagccaggg cagatccgtg cacagcacct 2640
tgccgtagaa gaacagcaag gccgccaatg cctgacgatg cgtggagacc gaaaccttgc 2700
gctcgttcgc cagccaggac agaaatgcct cgacttcgct gctgcccaag gttgccgggt 2760
gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc 2820
gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac 2880
gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg 2940
tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga 3000
tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca 3060
tcatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca 3120
tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg 3180
gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg 3240
aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga 3300
gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc 3360
gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag 3420
gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag 3480
aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac 3540
aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg 3600
ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg 3660
gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt 3720
atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg 3780
cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg 3840
tagtcggcaa ataatgtcta acaattcgtt caagccgacg ccgcttcgcg gcgcggctta 3900
actcaagcgt tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc 3960
tgcttttatt atttttaagc gtgcataata agccctacac aaattgggag atatatcatg 4020
aaaggctggc tttttcttgt tatcgcaata gttggcgaag taatcgcaac atccgcatta 4080
aaatctagcg agggctttac taagctgatc cggtggatga ccttttgaat gacctttaat 4140
agattatatt actaattaat tggggaccct agaggtcccc ttttttattt taaaaatttt 4200
ttcacaaaac ggtttacaag catacgttgg ccgattcatt aatgcagctg gcacgacagg 4260
tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat 4320
taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc 4380
ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct tgcatgcctg 4440
caggtcgacc gttaaatcta tcaccgcaag ggataaatat ctaacaccgt gcgtgttgac 4500
tattttacct ctggcggtga taatggttgc atgtactaat ctagataagg aatatagcca 4560
tgaccgcacc gattcaggat ctgcgtgatg caattgccct gctgcaacag catgataatc 4620
agtatctgga aaccgatcat ccggttgatc cgaatgcaga actggcaggc gtttatcgtc 4680
atattggtgc cggtggcacc gttaaacgtc cgacccgtat tggtccggca atgatgttta 4740
ataacattaa aggttatccg cacagccgta ttctggttgg tatgcatgca agccgtcagc 4800
gtgcagcact gctgctgggt tgtgaagcaa gtcagctggc actggaagtt ggtaaagcag 4860
ttaaaaaacc ggttgcaccg gtggttgttc cggcaagcag cgcaccgtgt caagagcaga 4920
tttttctggc agatgatccg gattttgatc tgcgtaccct gctgcctgca cataccaata 4980
ccccgattga tgcaggtccg tttttttgtc tgggtctggc cctggcaagc gatccggtgg 5040
atgcaagcct gaccgatgtt accattcatc gtctgtgtgt tcagggtcgt gatgaactga 5100
gcatgttcct ggcagcaggt cgccatattg aagtttttcg tcagaaagca gaagcagcag 5160
gtaaaccgct gccgattacc attaatatgg gtctggaccc agcaatctat attggcgcat 5220
gttttgaagc accgaccacc ccgtttggtt ataatgaact gggtgttgcc ggtgcactgc 5280
gtcagcgtcc ggttgaactg gttcagggtg ttagcgttcc ggaaaaagca attgcacgtg 5340
ccgaaattgt tattgaaggt gaactgctgc ctggtgttcg tgttcgtgaa gatcagcata 5400
ccaattcagg tcatgcaatg ccggaatttc cgggttattg tggtggtgca aatccgagcc 5460
tgccggttat taaagttaaa gccgttacca tgcgcaataa cgcaattctg caaaccctgg 5520
ttggtccggg tgaagaacat accaccctgg caggtctgcc gaccgaagca agcatttgga 5580
atgcagttga agcagcaatt ccgggttttc tgcaaaatgt ttatgcccat accgcaggcg 5640
gtggtaaatt tctgggtatt ctgcaagtga aaaaacgtca gcctgccgat gaaggtcgtc 5700
agggtcaggc agccctgctg gcgctggcaa cctatagcga actgaaaaat atcattctgg 5760
tggatgagga tgtggacatt tttgatagtg atgatattct gtgggcaatg accacccgta 5820
tgcagggtga tgttagcatt accaccattc cgggtattcg cggtcatcag ctggacccga 5880
gccagacacc ggaatattca ccgagcattc gtggtaatgg tattagctgc aaaaccatct 5940
ttgattgtac cgttccgtgg gcactgaaaa gccattttga acgtgcaccg tttgcagatg 6000
ttgatccgcg tccgtttgca cctgaatatt ttgcacgtct ggaaaaaaat cagggcagcg 6060
caaaataagc taataacagg cctgctggta atcgcaggaa tttttatttg gatggatccc 6120
cgggtaccga gctcgaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct 6180
ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc 6240
gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc 6300
ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact 6360
ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc 6420
gctgacgaat tc 6432
<210> 22
<211> 7294
<212> DNA
<213> 质粒pMG31;
<400> 22
gttgacagta agacgggtaa gcctgttgat gataccgctg ccttactggg tgcattagcc 60
agtctgaatg acctgtcacg ggataatccg aagtggtcag actggaaaat cagagggcag 120
gaactgctga acagcaaaaa gtcagatagc accacatagc agacccgcca taaaacgccc 180
tgagaagccc gtgacgggct tttcttgtat tatgggtagt ttccttgcat gaatccataa 240
aaggcgcctg tagtgccatt tacccccatt cactgccaga gccgtgagcg cagcgaactg 300
aatgtcacga aaaagacagc gactcaggtg cctgatggtc ggagacaaaa ggaatattca 360
gcgatttgcc cgagcttgcg agggtgctac ttaagccttt agggttttaa ggtctgtttt 420
gtagaggagc aaacagcgtt tgcgacatcc ttttgtaata ctgcggaact gactaaagta 480
gtgagttata cacagggctg ggatctattc tttttatctt tttttattct ttctttattc 540
tataaattat aaccacttga atataaacaa aaaaaacaca caaaggtcta gcggaattta 600
cagagggtct agcagaattt acaagttttc cagcaaaggt ctagcagaat ttacagatac 660
ccacaactca aaggaaaagg actagtaatt atcattgact agcccatctc aattggtata 720
gtgattaaaa tcacctagac caattgagat gtatgtctga attagttgtt ttcaaagcaa 780
atgaactagc gattagtcgc tatgacttaa cggagcatga aaccaagcta attttatgct 840
gtgtggcact actcaacccc acgattgaaa accctacaag gaaagaacgg acggtatcgt 900
tcacttataa ccaatacgct cagatgatga acatcagtag ggaaaatgct tatggtgtat 960
tagctaaagc aaccagagag ctgatgacga gaactgtgga aatcaggaat cctttggtta 1020
aaggctttga gattttccag tggacaaact atgccaagtt ctcaagcgaa aaattagaat 1080
tagtttttag tgaagagata ttgccttatc ttttccagtt aaaaaaattc ataaaatata 1140
atctggaaca tgttaagtct tttgaaaaca aatactctat gaggatttat gagtggttat 1200
taaaagaact aacacaaaag aaaactcaca aggcaaatat agagattagc cttgatgaat 1260
ttaagttcat gttaatgctt gaaaataact accatgagtt taaaaggctt aaccaatggg 1320
ttttgaaacc aataagtaaa gatttaaaca cttacagcaa tatgaaattg gtggttgata 1380
agcgaggccg cccgactgat acgttgattt tccaagttga actagataga caaatggatc 1440
tcgtaaccga acttgagaac aaccagataa aaatgaatgg tgacaaaata ccaacaacca 1500
ttacatcaga ttcctaccta cgtaacggac taagaaaaac actacacgat gctttaactg 1560
caaaaattca gctcaccagt tttgaggcaa aatttttgag tgacatgcaa agtaagcatg 1620
atctcaatgg ttcgttctca tggctcacgc aaaaacaacg aaccacacta gagaacatac 1680
tggctaaata cggaaggatc tgaggttctt atggctcttg tatctatcag tgaagcatca 1740
agactaacaa acaaaagtag aacaactgtt caccgttaga tatcaaaggg aaaactgtcc 1800
atatgcacag atgaaaacgg tgtaaaaaag atagatacat cagagctttt acgagttttt 1860
ggtgcattta aagctgttca ccatgaacag atcgacaatg taacagatga acagcatgta 1920
acacctaata gaacaggtga aaccagtaaa acaaagcaac tagaacatga aattgaacac 1980
ctgagacaac ttgttacagc tcaacagtca cacatagaca gcctgaaaca ggcgatgctg 2040
cttatcgaat caaagctgcc gacaacacgg gagccagtga cgcctcccgt ggggaaaaaa 2100
tcatggcaat tctggaagaa atagcgcttt cagccggcaa acctgaagcc ggatctgcga 2160
ttctgataac aaactagcaa caccagaaca gcccgtttgc gggcagcaaa acccgttatg 2220
cttgtaaacc gttttgtgaa aaaattttta aaataaaaaa ggggacctct agggtcccca 2280
attaattagt aatataatct attaaaggtc attcaaaagg tcatccaccg gatcaattcc 2340
cctgctcgcg caggctgggt gccaagctct cgggtaacat caaggcccga tccttggagc 2400
ccttgccctc ccgcacgatg atcgtgccgt gatcgaaatc cagatccttg acccgcagtt 2460
gcaaaccctc actgatccgc atgcccgttc catacagaag ctgggcgaac aaacgatgct 2520
cgccttccag aaaaccgagg atgcgaacca cttcatccgg ggtcagcacc accggcaagc 2580
gccgcgacgg ccgaggtctt ccgatctcct gaagccaggg cagatccgtg cacagcacct 2640
tgccgtagaa gaacagcaag gccgccaatg cctgacgatg cgtggagacc gaaaccttgc 2700
gctcgttcgc cagccaggac agaaatgcct cgacttcgct gctgcccaag gttgccgggt 2760
gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc 2820
gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac 2880
gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg 2940
tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga 3000
tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca 3060
tcatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca 3120
tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg 3180
gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg 3240
aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga 3300
gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc 3360
gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag 3420
gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag 3480
aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac 3540
aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg 3600
ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg 3660
gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt 3720
atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg 3780
cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg 3840
tagtcggcaa ataatgtcta acaattcgtt caagccgacg ccgcttcgcg gcgcggctta 3900
actcaagcgt tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc 3960
tgcttttatt atttttaagc gtgcataata agccctacac aaattgggag atatatcatg 4020
aaaggctggc tttttcttgt tatcgcaata gttggcgaag taatcgcaac atccgcatta 4080
aaatctagcg agggctttac taagctgatc cggtggatga ccttttgaat gacctttaat 4140
agattatatt actaattaat tggggaccct agaggtcccc ttttttattt taaaaatttt 4200
ttcacaaaac ggtttacaag catacgttgg ccgattcatt aatgcagctg gcacgacagg 4260
tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat 4320
taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc 4380
ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct tgctattgac 4440
gacagctatg gttcactgtc caccaaccaa aactgtgctc agtaccgcca atatttctcc 4500
cttgaggggt acaaagaggt gtccctagaa gagatccacg ctgtgtaaaa attttacaaa 4560
aaggtattga ctttccctac agggtgtgta ataatttaat tacaggcggg ggcaaccccg 4620
cctgttctgc agaggaggaa tatagccatg gaagtgaaaa tcttcaacac ccaggatgtt 4680
caggattttc tgcgtgttgc aagcggtctg gaacaagagg gtggtaatcc gcgtgttaaa 4740
caaattattc atcgtgttct gagcgacctg tataaagcaa ttgaagatct gaatatcacc 4800
agcgacgaat attgggcagg cgttgcatat ctgaatcagc tgggtgcaaa tcaagaagca 4860
ggtctgctga gtccgggtct gggttttgat cattatctgg atatgcgtat ggatgcagaa 4920
gatgcagcac tgggtattga aaatgcaaca ccgcgtacca ttgaaggtcc gctgtatgtt 4980
gcgggtgcac cggaaagcgt tggttatgca cgcatggatg atggtagcga tccgaatggt 5040
cataccctga ttctgcatgg caccattttt gatgcagatg gtaaaccgct gccgaatgca 5100
aaagttgaaa tttggcatgc aaacaccaaa ggcttttata gccattttga tccgaccggt 5160
gaacagcagg cctttaatat gcgtcgtagc attattaccg atgagaatgg tcagtatcgt 5220
gttcgtacca ttctgcctgc cggttatggt tgtcctccgg aaggtccgac ccagcaactg 5280
ctgaaccaac tgggtcgtca tggtaatcgt ccggcacata ttcattattt tgttagcgca 5340
gatggtcacc gtaaactgac cacccagatt aatgttgccg gtgatccgta tacctatgat 5400
gattttgcat atgccacccg tgaaggtctg gttgttgatg cagttgaaca taccgatccg 5460
gaagcaatta aagccaatga tgtggaaggt ccttttgccg aaatggtgtt tgatctgaaa 5520
ctgacccgtc tggttgatgg tgttgataat caggttgtgg atcgtccgcg tctggcagtt 5580
taatacacca aaatggttca aaattatcag gcgagtgatc atgatcactg gcctgttttt 5640
atttcaggga agggtggaga caattacgtg gataatcaga tcatccaaga aaccgtggat 5700
aaaattctga gcgttctgcc gaatcaggca ggtcagctgg cacgtctggt gcgtctgatg 5760
caatttgcat gcgatccgac cattaccgtt attggcaaat ataaccatgg taaaagccgt 5820
ctgctgaatg aactgattgg caccgatatc tttagcgttg cagataaacg tgaaaccatt 5880
cagctggccg aacataaaca ggatcaggtt cgttggctgg atgcacctgg tctggatgcc 5940
gatgttgcag cagttgatga tcgtcatgca tttgaagcag tttggaccca ggcagatatt 6000
cgtctgtttg ttcatagcgt tcgtgaaggt gaactggatg caaccgaaca ccatctgctg 6060
caacagctga ttgaagatgc cgatcatagc cgtcgtcaga ccattctggt tctgacccag 6120
attgatcaga ttccggatca gaccatcctg acacagatta aaaccagcat tgcacagcag 6180
gttccgaaac tggatatttg ggcagttagc gcaacccgtc atcgtcaggg cattgaaaac 6240
ggtaaaaccc tgctgatcga aaaaagcggt attggtgcac tgcgccatac cctggaacag 6300
gcactggcac aggtgccgag cgcacgtacc tatgaaaaaa atcgtctgct gtcagatctg 6360
caccatcagc tgaaacaact gctgctggat cagaaacatg ttctgcaaca actgcaacag 6420
acacagcaac agcagctgca tgattttgat accggtctga ttaacattct ggacaaaatt 6480
cgtgttgatc tggaaccgat tgtgaatatt gatggtcagg atcaagcact gaatccggat 6540
agctttgcaa ccatgtttaa aaacaccgca gcaaaacagc agcgtgccaa agttcagatt 6600
gcatatagcc gtgcatgcat tgaaatcaac agccatctga ttcgccatgg tgttgttggt 6660
ctgcctgcgg aacagcagac caccattaaa agcattgata ccgtgattgt tgccgtgttt 6720
ggtatcagcg ttaaatttcg tgatcagctg cgtgccctgt tttataccga taccgaacgt 6780
cagcgtctgc aacgtgaatt tcgtttctat tttgaaaaaa gtgccggtcg catgattctg 6840
gcagcaaaaa ttgaacagac catgcgtcag cagggctgta ttcagaatgc catgatggca 6900
ctgcaacaaa tggaaagcgc agcataaaaa cacggacgcc gcaaacggcg tccgaatttc 6960
ttggtcgact ctagaggatc cccgggtacc gagctcgaat tcactggccg tcgttttaca 7020
acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc 7080
tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg 7140
cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat 7200
ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca 7260
gccccgacac ccgccaacac ccgctgacga attc 7294
<210> 23
<211> 8952
<212> DNA
<213> 质粒pMG33;
<400> 23
gttgacagta agacgggtaa gcctgttgat gataccgctg ccttactggg tgcattagcc 60
agtctgaatg acctgtcacg ggataatccg aagtggtcag actggaaaat cagagggcag 120
gaactgctga acagcaaaaa gtcagatagc accacatagc agacccgcca taaaacgccc 180
tgagaagccc gtgacgggct tttcttgtat tatgggtagt ttccttgcat gaatccataa 240
aaggcgcctg tagtgccatt tacccccatt cactgccaga gccgtgagcg cagcgaactg 300
aatgtcacga aaaagacagc gactcaggtg cctgatggtc ggagacaaaa ggaatattca 360
gcgatttgcc cgagcttgcg agggtgctac ttaagccttt agggttttaa ggtctgtttt 420
gtagaggagc aaacagcgtt tgcgacatcc ttttgtaata ctgcggaact gactaaagta 480
gtgagttata cacagggctg ggatctattc tttttatctt tttttattct ttctttattc 540
tataaattat aaccacttga atataaacaa aaaaaacaca caaaggtcta gcggaattta 600
cagagggtct agcagaattt acaagttttc cagcaaaggt ctagcagaat ttacagatac 660
ccacaactca aaggaaaagg actagtaatt atcattgact agcccatctc aattggtata 720
gtgattaaaa tcacctagac caattgagat gtatgtctga attagttgtt ttcaaagcaa 780
atgaactagc gattagtcgc tatgacttaa cggagcatga aaccaagcta attttatgct 840
gtgtggcact actcaacccc acgattgaaa accctacaag gaaagaacgg acggtatcgt 900
tcacttataa ccaatacgct cagatgatga acatcagtag ggaaaatgct tatggtgtat 960
tagctaaagc aaccagagag ctgatgacga gaactgtgga aatcaggaat cctttggtta 1020
aaggctttga gattttccag tggacaaact atgccaagtt ctcaagcgaa aaattagaat 1080
tagtttttag tgaagagata ttgccttatc ttttccagtt aaaaaaattc ataaaatata 1140
atctggaaca tgttaagtct tttgaaaaca aatactctat gaggatttat gagtggttat 1200
taaaagaact aacacaaaag aaaactcaca aggcaaatat agagattagc cttgatgaat 1260
ttaagttcat gttaatgctt gaaaataact accatgagtt taaaaggctt aaccaatggg 1320
ttttgaaacc aataagtaaa gatttaaaca cttacagcaa tatgaaattg gtggttgata 1380
agcgaggccg cccgactgat acgttgattt tccaagttga actagataga caaatggatc 1440
tcgtaaccga acttgagaac aaccagataa aaatgaatgg tgacaaaata ccaacaacca 1500
ttacatcaga ttcctaccta cgtaacggac taagaaaaac actacacgat gctttaactg 1560
caaaaattca gctcaccagt tttgaggcaa aatttttgag tgacatgcaa agtaagcatg 1620
atctcaatgg ttcgttctca tggctcacgc aaaaacaacg aaccacacta gagaacatac 1680
tggctaaata cggaaggatc tgaggttctt atggctcttg tatctatcag tgaagcatca 1740
agactaacaa acaaaagtag aacaactgtt caccgttaga tatcaaaggg aaaactgtcc 1800
atatgcacag atgaaaacgg tgtaaaaaag atagatacat cagagctttt acgagttttt 1860
ggtgcattta aagctgttca ccatgaacag atcgacaatg taacagatga acagcatgta 1920
acacctaata gaacaggtga aaccagtaaa acaaagcaac tagaacatga aattgaacac 1980
ctgagacaac ttgttacagc tcaacagtca cacatagaca gcctgaaaca ggcgatgctg 2040
cttatcgaat caaagctgcc gacaacacgg gagccagtga cgcctcccgt ggggaaaaaa 2100
tcatggcaat tctggaagaa atagcgcttt cagccggcaa acctgaagcc ggatctgcga 2160
ttctgataac aaactagcaa caccagaaca gcccgtttgc gggcagcaaa acccgttatg 2220
cttgtaaacc gttttgtgaa aaaattttta aaataaaaaa ggggacctct agggtcccca 2280
attaattagt aatataatct attaaaggtc attcaaaagg tcatccaccg gatcaattcc 2340
cctgctcgcg caggctgggt gccaagctct cgggtaacat caaggcccga tccttggagc 2400
ccttgccctc ccgcacgatg atcgtgccgt gatcgaaatc cagatccttg acccgcagtt 2460
gcaaaccctc actgatccgc atgcccgttc catacagaag ctgggcgaac aaacgatgct 2520
cgccttccag aaaaccgagg atgcgaacca cttcatccgg ggtcagcacc accggcaagc 2580
gccgcgacgg ccgaggtctt ccgatctcct gaagccaggg cagatccgtg cacagcacct 2640
tgccgtagaa gaacagcaag gccgccaatg cctgacgatg cgtggagacc gaaaccttgc 2700
gctcgttcgc cagccaggac agaaatgcct cgacttcgct gctgcccaag gttgccgggt 2760
gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc 2820
gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac 2880
gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg 2940
tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga 3000
tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca 3060
tcatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca 3120
tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg 3180
gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg 3240
aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga 3300
gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc 3360
gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag 3420
gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag 3480
aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac 3540
aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg 3600
ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg 3660
gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt 3720
atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg 3780
cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg 3840
tagtcggcaa ataatgtcta acaattcgtt caagccgacg ccgcttcgcg gcgcggctta 3900
actcaagcgt tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc 3960
tgcttttatt atttttaagc gtgcataata agccctacac aaattgggag atatatcatg 4020
aaaggctggc tttttcttgt tatcgcaata gttggcgaag taatcgcaac atccgcatta 4080
aaatctagcg agggctttac taagctgatc cggtggatga ccttttgaat gacctttaat 4140
agattatatt actaattaat tggggaccct agaggtcccc ttttttattt taaaaatttt 4200
ttcacaaaac ggtttacaag catacgttgg ccgattcatt aatgcagctg gcacgacagg 4260
tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat 4320
taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc 4380
ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct tgctattgac 4440
gacagctatg gttcactgtc caccaaccaa aactgtgctc agtaccgcca atatttctcc 4500
cttgaggggt acaaagaggt gtccctagaa gagatccacg ctgtgtaaaa attttacaaa 4560
aaggtattga ctttccctac agggtgtgta ataatttaat tacaggcggg ggcaaccccg 4620
cctgttctgc agaggaggaa tatagccatg gaagtgaaaa tcttcaacac ccaggatgtt 4680
caggattttc tgcgtgttgc aagcggtctg gaacaagagg gtggtaatcc gcgtgttaaa 4740
caaattattc atcgtgttct gagcgacctg tataaagcaa ttgaagatct gaatatcacc 4800
agcgacgaat attgggcagg cgttgcatat ctgaatcagc tgggtgcaaa tcaagaagca 4860
ggtctgctga gtccgggtct gggttttgat cattatctgg atatgcgtat ggatgcagaa 4920
gatgcagcac tgggtattga aaatgcaaca ccgcgtacca ttgaaggtcc gctgtatgtt 4980
gcgggtgcac cggaaagcgt tggttatgca cgcatggatg atggtagcga tccgaatggt 5040
cataccctga ttctgcatgg caccattttt gatgcagatg gtaaaccgct gccgaatgca 5100
aaagttgaaa tttggcatgc aaacaccaaa ggcttttata gccattttga tccgaccggt 5160
gaacagcagg cctttaatat gcgtcgtagc attattaccg atgagaatgg tcagtatcgt 5220
gttcgtacca ttctgcctgc cggttatggt tgtcctccgg aaggtccgac ccagcaactg 5280
ctgaaccaac tgggtcgtca tggtaatcgt ccggcacata ttcattattt tgttagcgca 5340
gatggtcacc gtaaactgac cacccagatt aatgttgccg gtgatccgta tacctatgat 5400
gattttgcat atgccacccg tgaaggtctg gttgttgatg cagttgaaca taccgatccg 5460
gaagcaatta aagccaatga tgtggaaggt ccttttgccg aaatggtgtt tgatctgaaa 5520
ctgacccgtc tggttgatgg tgttgataat caggttgtgg atcgtccgcg tctggcagtt 5580
taatacacca aaatggttca aaattatcag gcgagtgatc atgatcactg gcctgttttt 5640
atttcaggga agggtggaga caattacgtg gataatcaga tcatccaaga aaccgtggat 5700
aaaattctga gcgttctgcc gaatcaggca ggtcagctgg cacgtctggt gcgtctgatg 5760
caatttgcat gcgatccgac cattaccgtt attggcaaat ataaccatgg taaaagccgt 5820
ctgctgaatg aactgattgg caccgatatc tttagcgttg cagataaacg tgaaaccatt 5880
cagctggccg aacataaaca ggatcaggtt cgttggctgg atgcacctgg tctggatgcc 5940
gatgttgcag cagttgatga tcgtcatgca tttgaagcag tttggaccca ggcagatatt 6000
cgtctgtttg ttcatagcgt tcgtgaaggt gaactggatg caaccgaaca ccatctgctg 6060
caacagctga ttgaagatgc cgatcatagc cgtcgtcaga ccattctggt tctgacccag 6120
attgatcaga ttccggatca gaccatcctg acacagatta aaaccagcat tgcacagcag 6180
gttccgaaac tggatatttg ggcagttagc gcaacccgtc atcgtcaggg cattgaaaac 6240
ggtaaaaccc tgctgatcga aaaaagcggt attggtgcac tgcgccatac cctggaacag 6300
gcactggcac aggtgccgag cgcacgtacc tatgaaaaaa atcgtctgct gtcagatctg 6360
caccatcagc tgaaacaact gctgctggat cagaaacatg ttctgcaaca actgcaacag 6420
acacagcaac agcagctgca tgattttgat accggtctga ttaacattct ggacaaaatt 6480
cgtgttgatc tggaaccgat tgtgaatatt gatggtcagg atcaagcact gaatccggat 6540
agctttgcaa ccatgtttaa aaacaccgca gcaaaacagc agcgtgccaa agttcagatt 6600
gcatatagcc gtgcatgcat tgaaatcaac agccatctga ttcgccatgg tgttgttggt 6660
ctgcctgcgg aacagcagac caccattaaa agcattgata ccgtgattgt tgccgtgttt 6720
ggtatcagcg ttaaatttcg tgatcagctg cgtgccctgt tttataccga taccgaacgt 6780
cagcgtctgc aacgtgaatt tcgtttctat tttgaaaaaa gtgccggtcg catgattctg 6840
gcagcaaaaa ttgaacagac catgcgtcag cagggctgta ttcagaatgc catgatggca 6900
ctgcaacaaa tggaaagcgc agcataaaaa cacggacgcc gcaaacggcg tccgaatttc 6960
ttggtcgacc gttaaatcta tcaccgcaag ggataaatat ctaacaccgt gcgtgttgac 7020
tattttacct ctggcggtga taatggttgc atgtactaat ctagataagg aatatagcca 7080
tgaccgcacc gattcaggat ctgcgtgatg caattgccct gctgcaacag catgataatc 7140
agtatctgga aaccgatcat ccggttgatc cgaatgcaga actggcaggc gtttatcgtc 7200
atattggtgc cggtggcacc gttaaacgtc cgacccgtat tggtccggca atgatgttta 7260
ataacattaa aggttatccg cacagccgta ttctggttgg tatgcatgca agccgtcagc 7320
gtgcagcact gctgctgggt tgtgaagcaa gtcagctggc actggaagtt ggtaaagcag 7380
ttaaaaaacc ggttgcaccg gtggttgttc cggcaagcag cgcaccgtgt caagagcaga 7440
tttttctggc agatgatccg gattttgatc tgcgtaccct gctgcctgca cataccaata 7500
ccccgattga tgcaggtccg tttttttgtc tgggtctggc cctggcaagc gatccggtgg 7560
atgcaagcct gaccgatgtt accattcatc gtctgtgtgt tcagggtcgt gatgaactga 7620
gcatgttcct ggcagcaggt cgccatattg aagtttttcg tcagaaagca gaagcagcag 7680
gtaaaccgct gccgattacc attaatatgg gtctggaccc agcaatctat attggcgcat 7740
gttttgaagc accgaccacc ccgtttggtt ataatgaact gggtgttgcc ggtgcactgc 7800
gtcagcgtcc ggttgaactg gttcagggtg ttagcgttcc ggaaaaagca attgcacgtg 7860
ccgaaattgt tattgaaggt gaactgctgc ctggtgttcg tgttcgtgaa gatcagcata 7920
ccaattcagg tcatgcaatg ccggaatttc cgggttattg tggtggtgca aatccgagcc 7980
tgccggttat taaagttaaa gccgttacca tgcgcaataa cgcaattctg caaaccctgg 8040
ttggtccggg tgaagaacat accaccctgg caggtctgcc gaccgaagca agcatttgga 8100
atgcagttga agcagcaatt ccgggttttc tgcaaaatgt ttatgcccat accgcaggcg 8160
gtggtaaatt tctgggtatt ctgcaagtga aaaaacgtca gcctgccgat gaaggtcgtc 8220
agggtcaggc agccctgctg gcgctggcaa cctatagcga actgaaaaat atcattctgg 8280
tggatgagga tgtggacatt tttgatagtg atgatattct gtgggcaatg accacccgta 8340
tgcagggtga tgttagcatt accaccattc cgggtattcg cggtcatcag ctggacccga 8400
gccagacacc ggaatattca ccgagcattc gtggtaatgg tattagctgc aaaaccatct 8460
ttgattgtac cgttccgtgg gcactgaaaa gccattttga acgtgcaccg tttgcagatg 8520
ttgatccgcg tccgtttgca cctgaatatt ttgcacgtct ggaaaaaaat cagggcagcg 8580
caaaataagc taataacagg cctgctggta atcgcaggaa tttttatttg gatggatccc 8640
cgggtaccga gctcgaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct 8700
ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc 8760
gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc 8820
ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact 8880
ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc 8940
gctgacgaat tc 8952
<210> 24
<211> 10630
<212> DNA
<213> 质粒pMG37;
<400> 24
gttgacagta agacgggtaa gcctgttgat gataccgctg ccttactggg tgcattagcc 60
agtctgaatg acctgtcacg ggataatccg aagtggtcag actggaaaat cagagggcag 120
gaactgctga acagcaaaaa gtcagatagc accacatagc agacccgcca taaaacgccc 180
tgagaagccc gtgacgggct tttcttgtat tatgggtagt ttccttgcat gaatccataa 240
aaggcgcctg tagtgccatt tacccccatt cactgccaga gccgtgagcg cagcgaactg 300
aatgtcacga aaaagacagc gactcaggtg cctgatggtc ggagacaaaa ggaatattca 360
gcgatttgcc cgagcttgcg agggtgctac ttaagccttt agggttttaa ggtctgtttt 420
gtagaggagc aaacagcgtt tgcgacatcc ttttgtaata ctgcggaact gactaaagta 480
gtgagttata cacagggctg ggatctattc tttttatctt tttttattct ttctttattc 540
tataaattat aaccacttga atataaacaa aaaaaacaca caaaggtcta gcggaattta 600
cagagggtct agcagaattt acaagttttc cagcaaaggt ctagcagaat ttacagatac 660
ccacaactca aaggaaaagg actagtaatt atcattgact agcccatctc aattggtata 720
gtgattaaaa tcacctagac caattgagat gtatgtctga attagttgtt ttcaaagcaa 780
atgaactagc gattagtcgc tatgacttaa cggagcatga aaccaagcta attttatgct 840
gtgtggcact actcaacccc acgattgaaa accctacaag gaaagaacgg acggtatcgt 900
tcacttataa ccaatacgct cagatgatga acatcagtag ggaaaatgct tatggtgtat 960
tagctaaagc aaccagagag ctgatgacga gaactgtgga aatcaggaat cctttggtta 1020
aaggctttga gattttccag tggacaaact atgccaagtt ctcaagcgaa aaattagaat 1080
tagtttttag tgaagagata ttgccttatc ttttccagtt aaaaaaattc ataaaatata 1140
atctggaaca tgttaagtct tttgaaaaca aatactctat gaggatttat gagtggttat 1200
taaaagaact aacacaaaag aaaactcaca aggcaaatat agagattagc cttgatgaat 1260
ttaagttcat gttaatgctt gaaaataact accatgagtt taaaaggctt aaccaatggg 1320
ttttgaaacc aataagtaaa gatttaaaca cttacagcaa tatgaaattg gtggttgata 1380
agcgaggccg cccgactgat acgttgattt tccaagttga actagataga caaatggatc 1440
tcgtaaccga acttgagaac aaccagataa aaatgaatgg tgacaaaata ccaacaacca 1500
ttacatcaga ttcctaccta cgtaacggac taagaaaaac actacacgat gctttaactg 1560
caaaaattca gctcaccagt tttgaggcaa aatttttgag tgacatgcaa agtaagcatg 1620
atctcaatgg ttcgttctca tggctcacgc aaaaacaacg aaccacacta gagaacatac 1680
tggctaaata cggaaggatc tgaggttctt atggctcttg tatctatcag tgaagcatca 1740
agactaacaa acaaaagtag aacaactgtt caccgttaga tatcaaaggg aaaactgtcc 1800
atatgcacag atgaaaacgg tgtaaaaaag atagatacat cagagctttt acgagttttt 1860
ggtgcattta aagctgttca ccatgaacag atcgacaatg taacagatga acagcatgta 1920
acacctaata gaacaggtga aaccagtaaa acaaagcaac tagaacatga aattgaacac 1980
ctgagacaac ttgttacagc tcaacagtca cacatagaca gcctgaaaca ggcgatgctg 2040
cttatcgaat caaagctgcc gacaacacgg gagccagtga cgcctcccgt ggggaaaaaa 2100
tcatggcaat tctggaagaa atagcgcttt cagccggcaa acctgaagcc ggatctgcga 2160
ttctgataac aaactagcaa caccagaaca gcccgtttgc gggcagcaaa acccgttatg 2220
cttgtaaacc gttttgtgaa aaaattttta aaataaaaaa ggggacctct agggtcccca 2280
attaattagt aatataatct attaaaggtc attcaaaagg tcatccaccg gatcaattcc 2340
cctgctcgcg caggctgggt gccaagctct cgggtaacat caaggcccga tccttggagc 2400
ccttgccctc ccgcacgatg atcgtgccgt gatcgaaatc cagatccttg acccgcagtt 2460
gcaaaccctc actgatccgc atgcccgttc catacagaag ctgggcgaac aaacgatgct 2520
cgccttccag aaaaccgagg atgcgaacca cttcatccgg ggtcagcacc accggcaagc 2580
gccgcgacgg ccgaggtctt ccgatctcct gaagccaggg cagatccgtg cacagcacct 2640
tgccgtagaa gaacagcaag gccgccaatg cctgacgatg cgtggagacc gaaaccttgc 2700
gctcgttcgc cagccaggac agaaatgcct cgacttcgct gctgcccaag gttgccgggt 2760
gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc 2820
gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac 2880
gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg 2940
tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga 3000
tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca 3060
tcatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca 3120
tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg 3180
gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg 3240
aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga 3300
gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc 3360
gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag 3420
gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag 3480
aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac 3540
aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg 3600
ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg 3660
gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt 3720
atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg 3780
cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg 3840
tagtcggcaa ataatgtcta acaattcgtt caagccgacg ccgcttcgcg gcgcggctta 3900
actcaagcgt tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc 3960
tgcttttatt atttttaagc gtgcataata agccctacac aaattgggag atatatcatg 4020
aaaggctggc tttttcttgt tatcgcaata gttggcgaag taatcgcaac atccgcatta 4080
aaatctagcg agggctttac taagctgatc cggtggatga ccttttgaat gacctttaat 4140
agattatatt actaattaat tggggaccct agaggtcccc ttttttattt taaaaatttt 4200
ttcacaaaac ggtttacaag catacgttgg ccgattcatt aatgcagctg gcacgacagg 4260
tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat 4320
taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc 4380
ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct tgctattgac 4440
gacagctatg gttcactgtc caccaaccaa aactgtgctc agtaccgcca atatttctcc 4500
cttgaggggt acaaagaggt gtccctagaa gagatccacg ctgtgtaaaa attttacaaa 4560
aaggtattga ctttccctac agggtgtgta ataatttaat tacaggcggg ggcaaccccg 4620
cctgttctgc agaggaggaa tatagccatg gaagtgaaaa tcttcaacac ccaggatgtt 4680
caggattttc tgcgtgttgc aagcggtctg gaacaagagg gtggtaatcc gcgtgttaaa 4740
caaattattc atcgtgttct gagcgacctg tataaagcaa ttgaagatct gaatatcacc 4800
agcgacgaat attgggcagg cgttgcatat ctgaatcagc tgggtgcaaa tcaagaagca 4860
ggtctgctga gtccgggtct gggttttgat cattatctgg atatgcgtat ggatgcagaa 4920
gatgcagcac tgggtattga aaatgcaaca ccgcgtacca ttgaaggtcc gctgtatgtt 4980
gcgggtgcac cggaaagcgt tggttatgca cgcatggatg atggtagcga tccgaatggt 5040
cataccctga ttctgcatgg caccattttt gatgcagatg gtaaaccgct gccgaatgca 5100
aaagttgaaa tttggcatgc aaacaccaaa ggcttttata gccattttga tccgaccggt 5160
gaacagcagg cctttaatat gcgtcgtagc attattaccg atgagaatgg tcagtatcgt 5220
gttcgtacca ttctgcctgc cggttatggt tgtcctccgg aaggtccgac ccagcaactg 5280
ctgaaccaac tgggtcgtca tggtaatcgt ccggcacata ttcattattt tgttagcgca 5340
gatggtcacc gtaaactgac cacccagatt aatgttgccg gtgatccgta tacctatgat 5400
gattttgcat atgccacccg tgaaggtctg gttgttgatg cagttgaaca taccgatccg 5460
gaagcaatta aagccaatga tgtggaaggt ccttttgccg aaatggtgtt tgatctgaaa 5520
ctgacccgtc tggttgatgg tgttgataat caggttgtgg atcgtccgcg tctggcagtt 5580
taatacacca aaatggttca aaattatcag gcgagtgatc atgatcactg gcctgttttt 5640
atttcaggga agggtggaga caattacgtg gataatcaga tcatccaaga aaccgtggat 5700
aaaattctga gcgttctgcc gaatcaggca ggtcagctgg cacgtctggt gcgtctgatg 5760
caatttgcat gcgatccgac cattaccgtt attggcaaat ataaccatgg taaaagccgt 5820
ctgctgaatg aactgattgg caccgatatc tttagcgttg cagataaacg tgaaaccatt 5880
cagctggccg aacataaaca ggatcaggtt cgttggctgg atgcacctgg tctggatgcc 5940
gatgttgcag cagttgatga tcgtcatgca tttgaagcag tttggaccca ggcagatatt 6000
cgtctgtttg ttcatagcgt tcgtgaaggt gaactggatg caaccgaaca ccatctgctg 6060
caacagctga ttgaagatgc cgatcatagc cgtcgtcaga ccattctggt tctgacccag 6120
attgatcaga ttccggatca gaccatcctg acacagatta aaaccagcat tgcacagcag 6180
gttccgaaac tggatatttg ggcagttagc gcaacccgtc atcgtcaggg cattgaaaac 6240
ggtaaaaccc tgctgatcga aaaaagcggt attggtgcac tgcgccatac cctggaacag 6300
gcactggcac aggtgccgag cgcacgtacc tatgaaaaaa atcgtctgct gtcagatctg 6360
caccatcagc tgaaacaact gctgctggat cagaaacatg ttctgcaaca actgcaacag 6420
acacagcaac agcagctgca tgattttgat accggtctga ttaacattct ggacaaaatt 6480
cgtgttgatc tggaaccgat tgtgaatatt gatggtcagg atcaagcact gaatccggat 6540
agctttgcaa ccatgtttaa aaacaccgca gcaaaacagc agcgtgccaa agttcagatt 6600
gcatatagcc gtgcatgcat tgaaatcaac agccatctga ttcgccatgg tgttgttggt 6660
ctgcctgcgg aacagcagac caccattaaa agcattgata ccgtgattgt tgccgtgttt 6720
ggtatcagcg ttaaatttcg tgatcagctg cgtgccctgt tttataccga taccgaacgt 6780
cagcgtctgc aacgtgaatt tcgtttctat tttgaaaaaa gtgccggtcg catgattctg 6840
gcagcaaaaa ttgaacagac catgcgtcag cagggctgta ttcagaatgc catgatggca 6900
ctgcaacaaa tggaaagcgc agcataaaaa cacggacgcc gcaaacggcg tccgaatttc 6960
ttggtcgacc gttaaatcta tcaccgcaag ggataaatat ctaacaccgt gcgtgttgac 7020
tattttacct ctggcggtga taatggttgc atgtactaat ctagataagg aatatagcca 7080
tgaccgcacc gattcaggat ctgcgtgatg caattgccct gctgcaacag catgataatc 7140
agtatctgga aaccgatcat ccggttgatc cgaatgcaga actggcaggc gtttatcgtc 7200
atattggtgc cggtggcacc gttaaacgtc cgacccgtat tggtccggca atgatgttta 7260
ataacattaa aggttatccg cacagccgta ttctggttgg tatgcatgca agccgtcagc 7320
gtgcagcact gctgctgggt tgtgaagcaa gtcagctggc actggaagtt ggtaaagcag 7380
ttaaaaaacc ggttgcaccg gtggttgttc cggcaagcag cgcaccgtgt caagagcaga 7440
tttttctggc agatgatccg gattttgatc tgcgtaccct gctgcctgca cataccaata 7500
ccccgattga tgcaggtccg tttttttgtc tgggtctggc cctggcaagc gatccggtgg 7560
atgcaagcct gaccgatgtt accattcatc gtctgtgtgt tcagggtcgt gatgaactga 7620
gcatgttcct ggcagcaggt cgccatattg aagtttttcg tcagaaagca gaagcagcag 7680
gtaaaccgct gccgattacc attaatatgg gtctggaccc agcaatctat attggcgcat 7740
gttttgaagc accgaccacc ccgtttggtt ataatgaact gggtgttgcc ggtgcactgc 7800
gtcagcgtcc ggttgaactg gttcagggtg ttagcgttcc ggaaaaagca attgcacgtg 7860
ccgaaattgt tattgaaggt gaactgctgc ctggtgttcg tgttcgtgaa gatcagcata 7920
ccaattcagg tcatgcaatg ccggaatttc cgggttattg tggtggtgca aatccgagcc 7980
tgccggttat taaagttaaa gccgttacca tgcgcaataa cgcaattctg caaaccctgg 8040
ttggtccggg tgaagaacat accaccctgg caggtctgcc gaccgaagca agcatttgga 8100
atgcagttga agcagcaatt ccgggttttc tgcaaaatgt ttatgcccat accgcaggcg 8160
gtggtaaatt tctgggtatt ctgcaagtga aaaaacgtca gcctgccgat gaaggtcgtc 8220
agggtcaggc agccctgctg gcgctggcaa cctatagcga actgaaaaat atcattctgg 8280
tggatgagga tgtggacatt tttgatagtg atgatattct gtgggcaatg accacccgta 8340
tgcagggtga tgttagcatt accaccattc cgggtattcg cggtcatcag ctggacccga 8400
gccagacacc ggaatattca ccgagcattc gtggtaatgg tattagctgc aaaaccatct 8460
ttgattgtac cgttccgtgg gcactgaaaa gccattttga acgtgcaccg tttgcagatg 8520
ttgatccgcg tccgtttgca cctgaatatt ttgcacgtct ggaaaaaaat cagggcagcg 8580
caaaataagc taataacagg cctgctggta atcgcaggaa tttttatttg gatggatccg 8640
cctacctagc ttccaagaaa gatatcctaa cagcacaaga gcggaaagat gttttgttct 8700
acatccagaa caacctctgc taaaattcct gaaaaatttt gcaaaaagtt gttgacttta 8760
tctacaaggt gtggtataat aatcttaaca acagcaggac gctcccgggt tgaggaaaac 8820
ctaatgaaac tgaccagcct gcgtgttagc ctgctggcac tgggtctggt taccagcggt 8880
tttgcagcag cagaaaccta taccgttgat cgttatcagg atgatagcga aaaaggtagc 8940
ctgcgttggg caattgaaca gagcaatgca aatagcgcac aagaaaacca gattctgatt 9000
caggcagttg gtaaagcacc gtatgttatc aaagttgata aaccgctgcc tccgattaaa 9060
agcagcgtta aaatcattgg caccgagtgg gataaaaccg gtgaatttat tgcaattgat 9120
ggcagcaact atatcaaagg cgaaggtgaa aaagcatgtc cgggtgcaaa tccgggtcag 9180
tatggcacca atgttcgtac catgaccctg cctggtctgg ttctgcaaga tgttaatggt 9240
gttaccctga aaggtctgga tgttcatcgt ttttgtattg gtgttctggt taatcgcagc 9300
agcaataacc tgattcagca taatcgtatc agcaacaatt atggtggtgc cggtgttatg 9360
attaccggtg atgatggtaa aggtaatccg accagcacca ccaccaataa taacaaagtt 9420
ctggataacg tgttcatcga taatggtgat ggtctggaac tgacccgtgg tgcagcattt 9480
aatctgattg caaataacct gtttaccagc acaaaagcca atccggaacc gagccagggt 9540
attgaaattc tgtggggtaa tgataatgcc gtggtgggta acaaattcga aaactattca 9600
gatggcctgc aaatcaattg gggtaaacgt aactatatcg cctataacga actgaccaat 9660
aacagcctgg gtttcaatct gacaggtgat ggtaacattt tcgacagcaa taaagtgcat 9720
ggtaaccgta ttggtattgc cattcgtagt gaaaaagatg ccaatgcacg tattaccctg 9780
accaaaaatc agatttggga taacggcaaa gatatcaaac gttgtgaagc cggtggtagc 9840
tgtgttccga atcagcgtct gggtgcaatt gtttttggtg ttccggcact ggaacatgaa 9900
ggttttgttg gtagccgtgg cggtggtgtt gttattgaac cggcaaaact gcaaaaaacc 9960
tgcacccagc cgaaccagca gaattgtaat gcaattccta atcagggtat tcaggcaccg 10020
aaactgacag ttagcaaaaa acagctgacc gttgaagtta aaggcacccc taatcagcgt 10080
tataatgtgg aattttttgg caatcgtaat gccagcagca gcgaagcaga acagtatctg 10140
ggtagcattg ttgttgttac cgatcatcag ggtctggcaa aagcaaattg ggctccgaaa 10200
gttagcatgc cgagcgttac cgcaaatgtg acagatcatc tgggtgcgac cagcgaactg 10260
agcagcgcag ttaaaatgcg ttaaatgcat gcgcgccgcg ttcgcgcggc gctttttttt 10320
ggtaccgagc tcgaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg 10380
cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga 10440
agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct 10500
gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct 10560
cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc 10620
tgacgaattc 10630
<210> 25
<211> 6452
<212> DNA
<213> 质粒pMG39;
<400> 25
gttgacagta agacgggtaa gcctgttgat gataccgctg ccttactggg tgcattagcc 60
agtctgaatg acctgtcacg ggataatccg aagtggtcag actggaaaat cagagggcag 120
gaactgctga acagcaaaaa gtcagatagc accacatagc agacccgcca taaaacgccc 180
tgagaagccc gtgacgggct tttcttgtat tatgggtagt ttccttgcat gaatccataa 240
aaggcgcctg tagtgccatt tacccccatt cactgccaga gccgtgagcg cagcgaactg 300
aatgtcacga aaaagacagc gactcaggtg cctgatggtc ggagacaaaa ggaatattca 360
gcgatttgcc cgagcttgcg agggtgctac ttaagccttt agggttttaa ggtctgtttt 420
gtagaggagc aaacagcgtt tgcgacatcc ttttgtaata ctgcggaact gactaaagta 480
gtgagttata cacagggctg ggatctattc tttttatctt tttttattct ttctttattc 540
tataaattat aaccacttga atataaacaa aaaaaacaca caaaggtcta gcggaattta 600
cagagggtct agcagaattt acaagttttc cagcaaaggt ctagcagaat ttacagatac 660
ccacaactca aaggaaaagg actagtaatt atcattgact agcccatctc aattggtata 720
gtgattaaaa tcacctagac caattgagat gtatgtctga attagttgtt ttcaaagcaa 780
atgaactagc gattagtcgc tatgacttaa cggagcatga aaccaagcta attttatgct 840
gtgtggcact actcaacccc acgattgaaa accctacaag gaaagaacgg acggtatcgt 900
tcacttataa ccaatacgct cagatgatga acatcagtag ggaaaatgct tatggtgtat 960
tagctaaagc aaccagagag ctgatgacga gaactgtgga aatcaggaat cctttggtta 1020
aaggctttga gattttccag tggacaaact atgccaagtt ctcaagcgaa aaattagaat 1080
tagtttttag tgaagagata ttgccttatc ttttccagtt aaaaaaattc ataaaatata 1140
atctggaaca tgttaagtct tttgaaaaca aatactctat gaggatttat gagtggttat 1200
taaaagaact aacacaaaag aaaactcaca aggcaaatat agagattagc cttgatgaat 1260
ttaagttcat gttaatgctt gaaaataact accatgagtt taaaaggctt aaccaatggg 1320
ttttgaaacc aataagtaaa gatttaaaca cttacagcaa tatgaaattg gtggttgata 1380
agcgaggccg cccgactgat acgttgattt tccaagttga actagataga caaatggatc 1440
tcgtaaccga acttgagaac aaccagataa aaatgaatgg tgacaaaata ccaacaacca 1500
ttacatcaga ttcctaccta cgtaacggac taagaaaaac actacacgat gctttaactg 1560
caaaaattca gctcaccagt tttgaggcaa aatttttgag tgacatgcaa agtaagcatg 1620
atctcaatgg ttcgttctca tggctcacgc aaaaacaacg aaccacacta gagaacatac 1680
tggctaaata cggaaggatc tgaggttctt atggctcttg tatctatcag tgaagcatca 1740
agactaacaa acaaaagtag aacaactgtt caccgttaga tatcaaaggg aaaactgtcc 1800
atatgcacag atgaaaacgg tgtaaaaaag atagatacat cagagctttt acgagttttt 1860
ggtgcattta aagctgttca ccatgaacag atcgacaatg taacagatga acagcatgta 1920
acacctaata gaacaggtga aaccagtaaa acaaagcaac tagaacatga aattgaacac 1980
ctgagacaac ttgttacagc tcaacagtca cacatagaca gcctgaaaca ggcgatgctg 2040
cttatcgaat caaagctgcc gacaacacgg gagccagtga cgcctcccgt ggggaaaaaa 2100
tcatggcaat tctggaagaa atagcgcttt cagccggcaa acctgaagcc ggatctgcga 2160
ttctgataac aaactagcaa caccagaaca gcccgtttgc gggcagcaaa acccgttatg 2220
cttgtaaacc gttttgtgaa aaaattttta aaataaaaaa ggggacctct agggtcccca 2280
attaattagt aatataatct attaaaggtc attcaaaagg tcatccaccg gatcaattcc 2340
cctgctcgcg caggctgggt gccaagctct cgggtaacat caaggcccga tccttggagc 2400
ccttgccctc ccgcacgatg atcgtgccgt gatcgaaatc cagatccttg acccgcagtt 2460
gcaaaccctc actgatccgc atgcccgttc catacagaag ctgggcgaac aaacgatgct 2520
cgccttccag aaaaccgagg atgcgaacca cttcatccgg ggtcagcacc accggcaagc 2580
gccgcgacgg ccgaggtctt ccgatctcct gaagccaggg cagatccgtg cacagcacct 2640
tgccgtagaa gaacagcaag gccgccaatg cctgacgatg cgtggagacc gaaaccttgc 2700
gctcgttcgc cagccaggac agaaatgcct cgacttcgct gctgcccaag gttgccgggt 2760
gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc 2820
gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac 2880
gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg 2940
tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga 3000
tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca 3060
tcatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca 3120
tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg 3180
gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg 3240
aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga 3300
gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc 3360
gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag 3420
gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag 3480
aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac 3540
aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg 3600
ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg 3660
gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt 3720
atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg 3780
cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg 3840
tagtcggcaa ataatgtcta acaattcgtt caagccgacg ccgcttcgcg gcgcggctta 3900
actcaagcgt tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc 3960
tgcttttatt atttttaagc gtgcataata agccctacac aaattgggag atatatcatg 4020
aaaggctggc tttttcttgt tatcgcaata gttggcgaag taatcgcaac atccgcatta 4080
aaatctagcg agggctttac taagctgatc cggtggatga ccttttgaat gacctttaat 4140
agattatatt actaattaat tggggaccct agaggtcccc ttttttattt taaaaatttt 4200
ttcacaaaac ggtttacaag catacgttgg ccgattcatt aatgcagctg gcacgacagg 4260
tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat 4320
taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc 4380
ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct tgcatgcctg 4440
caggtcgact ctagaggatc cgcctaccta gcttccaaga aagatatcct aacagcacaa 4500
gagcggaaag atgttttgtt ctacatccag aacaacctct gctaaaattc ctgaaaaatt 4560
ttgcaaaaag ttgttgactt tatctacaag gtgtggtata ataatcttaa caacagcagg 4620
acgctcccgg gttgaggaaa acctaatgaa actgaccagc ctgcgtgtta gcctgctggc 4680
actgggtctg gttaccagcg gttttgcagc agcagaaacc tataccgttg atcgttatca 4740
ggatgatagc gaaaaaggta gcctgcgttg ggcaattgaa cagagcaatg caaatagcgc 4800
acaagaaaac cagattctga ttcaggcagt tggtaaagca ccgtatgtta tcaaagttga 4860
taaaccgctg cctccgatta aaagcagcgt taaaatcatt ggcaccgagt gggataaaac 4920
cggtgaattt attgcaattg atggcagcaa ctatatcaaa ggcgaaggtg aaaaagcatg 4980
tccgggtgca aatccgggtc agtatggcac caatgttcgt accatgaccc tgcctggtct 5040
ggttctgcaa gatgttaatg gtgttaccct gaaaggtctg gatgttcatc gtttttgtat 5100
tggtgttctg gttaatcgca gcagcaataa cctgattcag cataatcgta tcagcaacaa 5160
ttatggtggt gccggtgtta tgattaccgg tgatgatggt aaaggtaatc cgaccagcac 5220
caccaccaat aataacaaag ttctggataa cgtgttcatc gataatggtg atggtctgga 5280
actgacccgt ggtgcagcat ttaatctgat tgcaaataac ctgtttacca gcacaaaagc 5340
caatccggaa ccgagccagg gtattgaaat tctgtggggt aatgataatg ccgtggtggg 5400
taacaaattc gaaaactatt cagatggcct gcaaatcaat tggggtaaac gtaactatat 5460
cgcctataac gaactgacca ataacagcct gggtttcaat ctgacaggtg atggtaacat 5520
tttcgacagc aataaagtgc atggtaaccg tattggtatt gccattcgta gtgaaaaaga 5580
tgccaatgca cgtattaccc tgaccaaaaa tcagatttgg gataacggca aagatatcaa 5640
acgttgtgaa gccggtggta gctgtgttcc gaatcagcgt ctgggtgcaa ttgtttttgg 5700
tgttccggca ctggaacatg aaggttttgt tggtagccgt ggcggtggtg ttgttattga 5760
accggcaaaa ctgcaaaaaa cctgcaccca gccgaaccag cagaattgta atgcaattcc 5820
taatcagggt attcaggcac cgaaactgac agttagcaaa aaacagctga ccgttgaagt 5880
taaaggcacc cctaatcagc gttataatgt ggaatttttt ggcaatcgta atgccagcag 5940
cagcgaagca gaacagtatc tgggtagcat tgttgttgtt accgatcatc agggtctggc 6000
aaaagcaaat tgggctccga aagttagcat gccgagcgtt accgcaaatg tgacagatca 6060
tctgggtgcg accagcgaac tgagcagcgc agttaaaatg cgttaaatgc atgcgcgccg 6120
cgttcgcgcg gcgctttttt ttggtaccga gctcgaattc actggccgtc gttttacaac 6180
gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt 6240
tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca 6300
gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt 6360
cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc 6420
cccgacaccc gccaacaccc gctgacgaat tc 6452
<210> 26
<211> 10012
<212> DNA
<213> 质粒pMG47F;
<400> 26
gttgacagta agacgggtaa gcctgttgat gataccgctg ccttactggg tgcattagcc 60
agtctgaatg acctgtcacg ggataatccg aagtggtcag actggaaaat cagagggcag 120
gaactgctga acagcaaaaa gtcagatagc accacatagc agacccgcca taaaacgccc 180
tgagaagccc gtgacgggct tttcttgtat tatgggtagt ttccttgcat gaatccataa 240
aaggcgcctg tagtgccatt tacccccatt cactgccaga gccgtgagcg cagcgaactg 300
aatgtcacga aaaagacagc gactcaggtg cctgatggtc ggagacaaaa ggaatattca 360
gcgatttgcc cgagcttgcg agggtgctac ttaagccttt agggttttaa ggtctgtttt 420
gtagaggagc aaacagcgtt tgcgacatcc ttttgtaata ctgcggaact gactaaagta 480
gtgagttata cacagggctg ggatctattc tttttatctt tttttattct ttctttattc 540
tataaattat aaccacttga atataaacaa aaaaaacaca caaaggtcta gcggaattta 600
cagagggtct agcagaattt acaagttttc cagcaaaggt ctagcagaat ttacagatac 660
ccacaactca aaggaaaagg actagtaatt atcattgact agcccatctc aattggtata 720
gtgattaaaa tcacctagac caattgagat gtatgtctga attagttgtt ttcaaagcaa 780
atgaactagc gattagtcgc tatgacttaa cggagcatga aaccaagcta attttatgct 840
gtgtggcact actcaacccc acgattgaaa accctacaag gaaagaacgg acggtatcgt 900
tcacttataa ccaatacgct cagatgatga acatcagtag ggaaaatgct tatggtgtat 960
tagctaaagc aaccagagag ctgatgacga gaactgtgga aatcaggaat cctttggtta 1020
aaggctttga gattttccag tggacaaact atgccaagtt ctcaagcgaa aaattagaat 1080
tagtttttag tgaagagata ttgccttatc ttttccagtt aaaaaaattc ataaaatata 1140
atctggaaca tgttaagtct tttgaaaaca aatactctat gaggatttat gagtggttat 1200
taaaagaact aacacaaaag aaaactcaca aggcaaatat agagattagc cttgatgaat 1260
ttaagttcat gttaatgctt gaaaataact accatgagtt taaaaggctt aaccaatggg 1320
ttttgaaacc aataagtaaa gatttaaaca cttacagcaa tatgaaattg gtggttgata 1380
agcgaggccg cccgactgat acgttgattt tccaagttga actagataga caaatggatc 1440
tcgtaaccga acttgagaac aaccagataa aaatgaatgg tgacaaaata ccaacaacca 1500
ttacatcaga ttcctaccta cgtaacggac taagaaaaac actacacgat gctttaactg 1560
caaaaattca gctcaccagt tttgaggcaa aatttttgag tgacatgcaa agtaagcatg 1620
atctcaatgg ttcgttctca tggctcacgc aaaaacaacg aaccacacta gagaacatac 1680
tggctaaata cggaaggatc tgaggttctt atggctcttg tatctatcag tgaagcatca 1740
agactaacaa acaaaagtag aacaactgtt caccgttaga tatcaaaggg aaaactgtcc 1800
atatgcacag atgaaaacgg tgtaaaaaag atagatacat cagagctttt acgagttttt 1860
ggtgcattta aagctgttca ccatgaacag atcgacaatg taacagatga acagcatgta 1920
acacctaata gaacaggtga aaccagtaaa acaaagcaac tagaacatga aattgaacac 1980
ctgagacaac ttgttacagc tcaacagtca cacatagaca gcctgaaaca ggcgatgctg 2040
cttatcgaat caaagctgcc gacaacacgg gagccagtga cgcctcccgt ggggaaaaaa 2100
tcatggcaat tctggaagaa atagcgcttt cagccggcaa acctgaagcc ggatctgcga 2160
ttctgataac aaactagcaa caccagaaca gcccgtttgc gggcagcaaa acccgttatg 2220
cttgtaaacc gttttgtgaa aaaattttta aaataaaaaa ggggacctct agggtcccca 2280
attaattagt aatataatct attaaaggtc attcaaaagg tcatccaccg gatcaattcc 2340
cctgctcgcg caggctgggt gccaagctct cgggtaacat caaggcccga tccttggagc 2400
ccttgccctc ccgcacgatg atcgtgccgt gatcgaaatc cagatccttg acccgcagtt 2460
gcaaaccctc actgatccgc atgcccgttc catacagaag ctgggcgaac aaacgatgct 2520
cgccttccag aaaaccgagg atgcgaacca cttcatccgg ggtcagcacc accggcaagc 2580
gccgcgacgg ccgaggtctt ccgatctcct gaagccaggg cagatccgtg cacagcacct 2640
tgccgtagaa gaacagcaag gccgccaatg cctgacgatg cgtggagacc gaaaccttgc 2700
gctcgttcgc cagccaggac agaaatgcct cgacttcgct gctgcccaag gttgccgggt 2760
gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc 2820
gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac 2880
gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg 2940
tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga 3000
tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca 3060
tcatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca 3120
tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg 3180
gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg 3240
aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga 3300
gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc 3360
gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag 3420
gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag 3480
aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac 3540
aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg 3600
ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg 3660
gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt 3720
atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg 3780
cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg 3840
tagtcggcaa ataatgtcta acaattcgtt caagccgacg ccgcttcgcg gcgcggctta 3900
actcaagcgt tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc 3960
tgcttttatt atttttaagc gtgcataata agccctacac aaattgggag atatatcatg 4020
aaaggctggc tttttcttgt tatcgcaata gttggcgaag taatcgcaac atccgcatta 4080
aaatctagcg agggctttac taagctgatc cggtggatga ccttttgaat gacctttaat 4140
agattatatt actaattaat tggggaccct agaggtcccc ttttttattt taaaaatttt 4200
ttcacaaaac ggtttacaag catacgttgg ccgattcatt aatgcagctg gcacgacagg 4260
tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat 4320
taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc 4380
ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct tgctattgac 4440
gacagctatg gttcactgtc caccaaccaa aactgtgctc agtaccgcca atatttctcc 4500
cttgaggggt acaaagaggt gtccctagaa gagatccacg ctgtgtaaaa attttacaaa 4560
aaggtattga ctttccctac agggtgtgta ataatttaat tacaggcggg ggcaaccccg 4620
cctgttctgc agaggaggaa tatagccatg gaagtgaaaa tcttcaacac ccaggatgtt 4680
caggattttc tgcgtgttgc aagcggtctg gaacaagagg gtggtaatcc gcgtgttaaa 4740
caaattattc atcgtgttct gagcgacctg tataaagcaa ttgaagatct gaatatcacc 4800
agcgacgaat attgggcagg cgttgcatat ctgaatcagc tgggtgcaaa tcaagaagca 4860
ggtctgctga gtccgggtct gggttttgat cattatctgg atatgcgtat ggatgcagaa 4920
gatgcagcac tgggtattga aaatgcaaca ccgcgtacca ttgaaggtcc gctgtatgtt 4980
gcgggtgcac cggaaagcgt tggttatgca cgcatggatg atggtagcga tccgaatggt 5040
cataccctga ttctgcatgg caccattttt gatgcagatg gtaaaccgct gccgaatgca 5100
aaagttgaaa tttggcatgc aaacaccaaa ggcttttata gccattttga tccgaccggt 5160
gaacagcagg cctttaatat gcgtcgtagc attattaccg atgagaatgg tcagtatcgt 5220
gttcgtacca ttctgcctgc cggttatggt tgtcctccgg aaggtccgac ccagcaactg 5280
ctgaaccaac tgggtcgtca tggtaatcgt ccggcacata ttcattattt tgttagcgca 5340
gatggtcacc gtaaactgac cacccagatt aatgttgccg gtgatccgta tacctatgat 5400
gattttgcat atgccacccg tgaaggtctg gttgttgatg cagttgaaca taccgatccg 5460
gaagcaatta aagccaatga tgtggaaggt ccttttgccg aaatggtgtt tgatctgaaa 5520
ctgacccgtc tggttgatgg tgttgataat caggttgtgg atcgtccgcg tctggcagtt 5580
taatacacca aaatggttca aaattatcag gcgagtgatc atgatcactg gcctgttttt 5640
atttcaggga agggtggaga caattacgtg gataatcaga tcatccaaga aaccgtggat 5700
aaaattctga gcgttctgcc gaatcaggca ggtcagctgg cacgtctggt gcgtctgatg 5760
caatttgcat gcgatccgac cattaccgtt attggcaaat ataaccatgg taaaagccgt 5820
ctgctgaatg aactgattgg caccgatatc tttagcgttg cagataaacg tgaaaccatt 5880
cagctggccg aacataaaca ggatcaggtt cgttggctgg atgcacctgg tctggatgcc 5940
gatgttgcag cagttgatga tcgtcatgca tttgaagcag tttggaccca ggcagatatt 6000
cgtctgtttg ttcatagcgt tcgtgaaggt gaactggatg caaccgaaca ccatctgctg 6060
caacagctga ttgaagatgc cgatcatagc cgtcgtcaga ccattctggt tctgacccag 6120
attgatcaga ttccggatca gaccatcctg acacagatta aaaccagcat tgcacagcag 6180
gttccgaaac tggatatttg ggcagttagc gcaacccgtc atcgtcaggg cattgaaaac 6240
ggtaaaaccc tgctgatcga aaaaagcggt attggtgcac tgcgccatac cctggaacag 6300
gcactggcac aggtgccgag cgcacgtacc tatgaaaaaa atcgtctgct gtcagatctg 6360
caccatcagc tgaaacaact gctgctggat cagaaacatg ttctgcaaca actgcaacag 6420
acacagcaac agcagctgca tgattttgat accggtctga ttaacattct ggacaaaatt 6480
cgtgttgatc tggaaccgat tgtgaatatt gatggtcagg atcaagcact gaatccggat 6540
agctttgcaa ccatgtttaa aaacaccgca gcaaaacagc agcgtgccaa agttcagatt 6600
gcatatagcc gtgcatgcat tgaaatcaac agccatctga ttcgccatgg tgttgttggt 6660
ctgcctgcgg aacagcagac caccattaaa agcattgata ccgtgattgt tgccgtgttt 6720
ggtatcagcg ttaaatttcg tgatcagctg cgtgccctgt tttataccga taccgaacgt 6780
cagcgtctgc aacgtgaatt tcgtttctat tttgaaaaaa gtgccggtcg catgattctg 6840
gcagcaaaaa ttgaacagac catgcgtcag cagggctgta ttcagaatgc catgatggca 6900
ctgcaacaaa tggaaagcgc agcataaaaa cacggacgcc gcaaacggcg tccgaatttc 6960
ttggtcgacc gttaaatcta tcaccgcaag ggataaatat ctaacaccgt gcgtgttgac 7020
tattttacct ctggcggtga taatggttgc atgtactaat ctagataagg aatatagcca 7080
tgaccgcacc gattcaggat ctgcgtgatg caattgccct gctgcaacag catgataatc 7140
agtatctgga aaccgatcat ccggttgatc cgaatgcaga actggcaggc gtttatcgtc 7200
atattggtgc cggtggcacc gttaaacgtc cgacccgtat tggtccggca atgatgttta 7260
ataacattaa aggttatccg cacagccgta ttctggttgg tatgcatgca agccgtcagc 7320
gtgcagcact gctgctgggt tgtgaagcaa gtcagctggc actggaagtt ggtaaagcag 7380
ttaaaaaacc ggttgcaccg gtggttgttc cggcaagcag cgcaccgtgt caagagcaga 7440
tttttctggc agatgatccg gattttgatc tgcgtaccct gctgcctgca cataccaata 7500
ccccgattga tgcaggtccg tttttttgtc tgggtctggc cctggcaagc gatccggtgg 7560
atgcaagcct gaccgatgtt accattcatc gtctgtgtgt tcagggtcgt gatgaactga 7620
gcatgttcct ggcagcaggt cgccatattg aagtttttcg tcagaaagca gaagcagcag 7680
gtaaaccgct gccgattacc attaatatgg gtctggaccc agcaatctat attggcgcat 7740
gttttgaagc accgaccacc ccgtttggtt ataatgaact gggtgttgcc ggtgcactgc 7800
gtcagcgtcc ggttgaactg gttcagggtg ttagcgttcc ggaaaaagca attgcacgtg 7860
ccgaaattgt tattgaaggt gaactgctgc ctggtgttcg tgttcgtgaa gatcagcata 7920
ccaattcagg tcatgcaatg ccggaatttc cgggttattg tggtggtgca aatccgagcc 7980
tgccggttat taaagttaaa gccgttacca tgcgcaataa cgcaattctg caaaccctgg 8040
ttggtccggg tgaagaacat accaccctgg caggtctgcc gaccgaagca agcatttgga 8100
atgcagttga agcagcaatt ccgggttttc tgcaaaatgt ttatgcccat accgcaggcg 8160
gtggtaaatt tctgggtatt ctgcaagtga aaaaacgtca gcctgccgat gaaggtcgtc 8220
agggtcaggc agccctgctg gcgctggcaa cctatagcga actgaaaaat atcattctgg 8280
tggatgagga tgtggacatt tttgatagtg atgatattct gtgggcaatg accacccgta 8340
tgcagggtga tgttagcatt accaccattc cgggtattcg cggtcatcag ctggacccga 8400
gccagacacc ggaatattca ccgagcattc gtggtaatgg tattagctgc aaaaccatct 8460
ttgattgtac cgttccgtgg gcactgaaaa gccattttga acgtgcaccg tttgcagatg 8520
ttgatccgcg tccgtttgca cctgaatatt ttgcacgtct ggaaaaaaat cagggcagcg 8580
caaaataagc taataacagg cctgctggta atcgcaggaa tttttatttg gatggatccg 8640
cctacctagc ttccaagaaa gatatcctaa cagcacaaga gcggaaagat gttttgttct 8700
acatccagaa caacctctgc taaaattcct gaaaaatttt gcaaaaagtt gttgacttta 8760
tctacaaggt gtggtataat aatcttaaca acagcaggac gctcccgggt tgaggaaaac 8820
ctaatgaaat atagcctgtg caccattagc tttcgtcacc agctgattag ctttaccgat 8880
attgttcagt ttgcctatga aaacggcttt gaaggtattg aactgtgggg cacccatgca 8940
cagaatctgt atatgcaaga atatgaaacc accgaacgtg aactgaattg cctgaaagat 9000
aaaaccctgg aaattaccat gatcagcgat tatctggata ttagcctgag cgcagatttt 9060
gaaaaaacca tcgaaaaatg tgaacagctg gcaattctgg ccaattggtt taaaacgaac 9120
aaaattcgta cctttgccgg tcagaaaggt agtgcagatt ttagccagca agaacgtcaa 9180
gagtatgtga atcgtattcg catgatttgt gaactgtttg cccagcataa tatgtatgtt 9240
ctgctggaaa cccatccgaa taccctgacc gataccctgc cgagcaccct ggaactgctg 9300
ggtgaagttg atcatccgaa tctgaaaatc aacctggatt ttctgcatat ctgggaaagc 9360
ggtgcagatc cggttgatag ctttcagcag ctgcgtccgt ggattcagca ttatcacttt 9420
aaaaacatta gcagcgcaga ctatctgcat gtgtttgaac cgaataatgt ttatgcagca 9480
gcaggtaatc gtaccggtat ggttccgctg tttgaaggca ttgttaacta tgatgaaatc 9540
atccaagaag tgcgcgatac cgatcatttt gcaagcctgg aatggtttgg tcataacgca 9600
aaagatattc tgaaagccga aatgaaagtg ctgaccaatc gtaatctgga agttgttacc 9660
agctaaatgc atgcgcgccg cgttcgcgcg gcgctttttt ttggtaccga gctcgaattc 9720
actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg 9780
ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg 9840
cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct 9900
tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga 9960
tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgaat tc 10012
<210> 27
<211> 10249
<212> DNA
<213> 质粒pMG70;
<400> 27
gttgacagta agacgggtaa gcctgttgat gataccgctg ccttactggg tgcattagcc 60
agtctgaatg acctgtcacg ggataatccg aagtggtcag actggaaaat cagagggcag 120
gaactgctga acagcaaaaa gtcagatagc accacatagc agacccgcca taaaacgccc 180
tgagaagccc gtgacgggct tttcttgtat tatgggtagt ttccttgcat gaatccataa 240
aaggcgcctg tagtgccatt tacccccatt cactgccaga gccgtgagcg cagcgaactg 300
aatgtcacga aaaagacagc gactcaggtg cctgatggtc ggagacaaaa ggaatattca 360
gcgatttgcc cgagcttgcg agggtgctac ttaagccttt agggttttaa ggtctgtttt 420
gtagaggagc aaacagcgtt tgcgacatcc ttttgtaata ctgcggaact gactaaagta 480
gtgagttata cacagggctg ggatctattc tttttatctt tttttattct ttctttattc 540
tataaattat aaccacttga atataaacaa aaaaaacaca caaaggtcta gcggaattta 600
cagagggtct agcagaattt acaagttttc cagcaaaggt ctagcagaat ttacagatac 660
ccacaactca aaggaaaagg actagtaatt atcattgact agcccatctc aattggtata 720
gtgattaaaa tcacctagac caattgagat gtatgtctga attagttgtt ttcaaagcaa 780
atgaactagc gattagtcgc tatgacttaa cggagcatga aaccaagcta attttatgct 840
gtgtggcact actcaacccc acgattgaaa accctacaag gaaagaacgg acggtatcgt 900
tcacttataa ccaatacgct cagatgatga acatcagtag ggaaaatgct tatggtgtat 960
tagctaaagc aaccagagag ctgatgacga gaactgtgga aatcaggaat cctttggtta 1020
aaggctttga gattttccag tggacaaact atgccaagtt ctcaagcgaa aaattagaat 1080
tagtttttag tgaagagata ttgccttatc ttttccagtt aaaaaaattc ataaaatata 1140
atctggaaca tgttaagtct tttgaaaaca aatactctat gaggatttat gagtggttat 1200
taaaagaact aacacaaaag aaaactcaca aggcaaatat agagattagc cttgatgaat 1260
ttaagttcat gttaatgctt gaaaataact accatgagtt taaaaggctt aaccaatggg 1320
ttttgaaacc aataagtaaa gatttaaaca cttacagcaa tatgaaattg gtggttgata 1380
agcgaggccg cccgactgat acgttgattt tccaagttga actagataga caaatggatc 1440
tcgtaaccga acttgagaac aaccagataa aaatgaatgg tgacaaaata ccaacaacca 1500
ttacatcaga ttcctaccta cgtaacggac taagaaaaac actacacgat gctttaactg 1560
caaaaattca gctcaccagt tttgaggcaa aatttttgag tgacatgcaa agtaagcatg 1620
atctcaatgg ttcgttctca tggctcacgc aaaaacaacg aaccacacta gagaacatac 1680
tggctaaata cggaaggatc tgaggttctt atggctcttg tatctatcag tgaagcatca 1740
agactaacaa acaaaagtag aacaactgtt caccgttaga tatcaaaggg aaaactgtcc 1800
atatgcacag atgaaaacgg tgtaaaaaag atagatacat cagagctttt acgagttttt 1860
ggtgcattta aagctgttca ccatgaacag atcgacaatg taacagatga acagcatgta 1920
acacctaata gaacaggtga aaccagtaaa acaaagcaac tagaacatga aattgaacac 1980
ctgagacaac ttgttacagc tcaacagtca cacatagaca gcctgaaaca ggcgatgctg 2040
cttatcgaat caaagctgcc gacaacacgg gagccagtga cgcctcccgt ggggaaaaaa 2100
tcatggcaat tctggaagaa atagcgcttt cagccggcaa acctgaagcc ggatctgcga 2160
ttctgataac aaactagcaa caccagaaca gcccgtttgc gggcagcaaa acccgttatg 2220
cttgtaaacc gttttgtgaa aaaattttta aaataaaaaa ggggacctct agggtcccca 2280
attaattagt aatataatct attaaaggtc attcaaaagg tcatccaccg gatcaattcc 2340
cctgctcgcg caggctgggt gccaagctct cgggtaacat caaggcccga tccttggagc 2400
ccttgccctc ccgcacgatg atcgtgccgt gatcgaaatc cagatccttg acccgcagtt 2460
gcaaaccctc actgatccgc atgcccgttc catacagaag ctgggcgaac aaacgatgct 2520
cgccttccag aaaaccgagg atgcgaacca cttcatccgg ggtcagcacc accggcaagc 2580
gccgcgacgg ccgaggtctt ccgatctcct gaagccaggg cagatccgtg cacagcacct 2640
tgccgtagaa gaacagcaag gccgccaatg cctgacgatg cgtggagacc gaaaccttgc 2700
gctcgttcgc cagccaggac agaaatgcct cgacttcgct gctgcccaag gttgccgggt 2760
gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc 2820
gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac 2880
gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg 2940
tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga 3000
tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca 3060
tcatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca 3120
tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg 3180
gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg 3240
aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga 3300
gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc 3360
gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag 3420
gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag 3480
aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac 3540
aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg 3600
ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg 3660
gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt 3720
atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg 3780
cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg 3840
tagtcggcaa ataatgtcta acaattcgtt caagccgacg ccgcttcgcg gcgcggctta 3900
actcaagcgt tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc 3960
tgcttttatt atttttaagc gtgcataata agccctacac aaattgggag atatatcatg 4020
aaaggctggc tttttcttgt tatcgcaata gttggcgaag taatcgcaac atccgcatta 4080
aaatctagcg agggctttac taagctgatc cggtggatga ccttttgaat gacctttaat 4140
agattatatt actaattaat tggggaccct agaggtcccc ttttttattt taaaaatttt 4200
ttcacaaaac ggtttacaag catacgttgg ccgattcatt aatgcagctg gcacgacagg 4260
tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat 4320
taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc 4380
ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct tgctattgac 4440
gacagctatg gttcactgtc caccaaccaa aactgtgctc agtaccgcca atatttctcc 4500
cttgaggggt acaaagaggt gtccctagaa gagatccacg ctgtgtaaaa attttacaaa 4560
aaggtattga ctttccctac agggtgtgta ataatttaat tacaggcggg ggcaaccccg 4620
cctgttctgc agaggaggaa tatagccatg gaagtgaaaa tcttcaacac ccaggatgtt 4680
caggattttc tgcgtgttgc aagcggtctg gaacaagagg gtggtaatcc gcgtgttaaa 4740
caaattattc atcgtgttct gagcgacctg tataaagcaa ttgaagatct gaatatcacc 4800
agcgacgaat attgggcagg cgttgcatat ctgaatcagc tgggtgcaaa tcaagaagca 4860
ggtctgctga gtccgggtct gggttttgat cattatctgg atatgcgtat ggatgcagaa 4920
gatgcagcac tgggtattga aaatgcaaca ccgcgtacca ttgaaggtcc gctgtatgtt 4980
gcgggtgcac cggaaagcgt tggttatgca cgcatggatg atggtagcga tccgaatggt 5040
cataccctga ttctgcatgg caccattttt gatgcagatg gtaaaccgct gccgaatgca 5100
aaagttgaaa tttggcatgc aaacaccaaa ggcttttata gccattttga tccgaccggt 5160
gaacagcagg cctttaatat gcgtcgtagc attattaccg atgagaatgg tcagtatcgt 5220
gttcgtacca ttctgcctgc cggttatggt tgtcctccgg aaggtccgac ccagcaactg 5280
ctgaaccaac tgggtcgtca tggtaatcgt ccggcacata ttcattattt tgttagcgca 5340
gatggtcacc gtaaactgac cacccagatt aatgttgccg gtgatccgta tacctatgat 5400
gattttgcat atgccacccg tgaaggtctg gttgttgatg cagttgaaca taccgatccg 5460
gaagcaatta aagccaatga tgtggaaggt ccttttgccg aaatggtgtt tgatctgaaa 5520
ctgacccgtc tggttgatgg tgttgataat caggttgtgg atcgtccgcg tctggcagtt 5580
taatacacca aaatggttca aaattatcag gcgagtgatc atgatcactg gcctgttttt 5640
atttcaggga agggtggaga caattacgtg gataatcaga tcatccaaga aaccgtggat 5700
aaaattctga gcgttctgcc gaatcaggca ggtcagctgg cacgtctggt gcgtctgatg 5760
caatttgcat gcgatccgac cattaccgtt attggcaaat ataaccatgg taaaagccgt 5820
ctgctgaatg aactgattgg caccgatatc tttagcgttg cagataaacg tgaaaccatt 5880
cagctggccg aacataaaca ggatcaggtt cgttggctgg atgcacctgg tctggatgcc 5940
gatgttgcag cagttgatga tcgtcatgca tttgaagcag tttggaccca ggcagatatt 6000
cgtctgtttg ttcatagcgt tcgtgaaggt gaactggatg caaccgaaca ccatctgctg 6060
caacagctga ttgaagatgc cgatcatagc cgtcgtcaga ccattctggt tctgacccag 6120
attgatcaga ttccggatca gaccatcctg acacagatta aaaccagcat tgcacagcag 6180
gttccgaaac tggatatttg ggcagttagc gcaacccgtc atcgtcaggg cattgaaaac 6240
ggtaaaaccc tgctgatcga aaaaagcggt attggtgcac tgcgccatac cctggaacag 6300
gcactggcac aggtgccgag cgcacgtacc tatgaaaaaa atcgtctgct gtcagatctg 6360
caccatcagc tgaaacaact gctgctggat cagaaacatg ttctgcaaca actgcaacag 6420
acacagcaac agcagctgca tgattttgat accggtctga ttaacattct ggacaaaatt 6480
cgtgttgatc tggaaccgat tgtgaatatt gatggtcagg atcaagcact gaatccggat 6540
agctttgcaa ccatgtttaa aaacaccgca gcaaaacagc agcgtgccaa agttcagatt 6600
gcatatagcc gtgcatgcat tgaaatcaac agccatctga ttcgccatgg tgttgttggt 6660
ctgcctgcgg aacagcagac caccattaaa agcattgata ccgtgattgt tgccgtgttt 6720
ggtatcagcg ttaaatttcg tgatcagctg cgtgccctgt tttataccga taccgaacgt 6780
cagcgtctgc aacgtgaatt tcgtttctat tttgaaaaaa gtgccggtcg catgattctg 6840
gcagcaaaaa ttgaacagac catgcgtcag cagggctgta ttcagaatgc catgatggca 6900
ctgcaacaaa tggaaagcgc agcataaaaa cacggacgcc gcaaacggcg tccgaatttc 6960
ttggtcgacc gttaaatcta tcaccgcaag ggataaatat ctaacaccgt gcgtgttgac 7020
tattttacct ctggcggtga taatggttgc atgtactaat ctagataagg aatatagcca 7080
tgaccgcacc gattcaggat ctgcgtgatg caattgccct gctgcaacag catgataatc 7140
agtatctgga aaccgatcat ccggttgatc cgaatgcaga actggcaggc gtttatcgtc 7200
atattggtgc cggtggcacc gttaaacgtc cgacccgtat tggtccggca atgatgttta 7260
ataacattaa aggttatccg cacagccgta ttctggttgg tatgcatgca agccgtcagc 7320
gtgcagcact gctgctgggt tgtgaagcaa gtcagctggc actggaagtt ggtaaagcag 7380
ttaaaaaacc ggttgcaccg gtggttgttc cggcaagcag cgcaccgtgt caagagcaga 7440
tttttctggc agatgatccg gattttgatc tgcgtaccct gctgcctgca cataccaata 7500
ccccgattga tgcaggtccg tttttttgtc tgggtctggc cctggcaagc gatccggtgg 7560
atgcaagcct gaccgatgtt accattcatc gtctgtgtgt tcagggtcgt gatgaactga 7620
gcatgttcct ggcagcaggt cgccatattg aagtttttcg tcagaaagca gaagcagcag 7680
gtaaaccgct gccgattacc attaatatgg gtctggaccc agcaatctat attggcgcat 7740
gttttgaagc accgaccacc ccgtttggtt ataatgaact gggtgttgcc ggtgcactgc 7800
gtcagcgtcc ggttgaactg gttcagggtg ttagcgttcc ggaaaaagca attgcacgtg 7860
ccgaaattgt tattgaaggt gaactgctgc ctggtgttcg tgttcgtgaa gatcagcata 7920
ccaattcagg tcatgcaatg ccggaatttc cgggttattg tggtggtgca aatccgagcc 7980
tgccggttat taaagttaaa gccgttacca tgcgcaataa cgcaattctg caaaccctgg 8040
ttggtccggg tgaagaacat accaccctgg caggtctgcc gaccgaagca agcatttgga 8100
atgcagttga agcagcaatt ccgggttttc tgcaaaatgt ttatgcccat accgcaggcg 8160
gtggtaaatt tctgggtatt ctgcaagtga aaaaacgtca gcctgccgat gaaggtcgtc 8220
agggtcaggc agccctgctg gcgctggcaa cctatagcga actgaaaaat atcattctgg 8280
tggatgagga tgtggacatt tttgatagtg atgatattct gtgggcaatg accacccgta 8340
tgcagggtga tgttagcatt accaccattc cgggtattcg cggtcatcag ctggacccga 8400
gccagacacc ggaatattca ccgagcattc gtggtaatgg tattagctgc aaaaccatct 8460
ttgattgtac cgttccgtgg gcactgaaaa gccattttga acgtgcaccg tttgcagatg 8520
ttgatccgcg tccgtttgca cctgaatatt ttgcacgtct ggaaaaaaat cagggcagcg 8580
caaaataagc taataacagg cctgctggta atcgcaggaa tttttatttg gatggatccg 8640
cctacctagc ttccaagaaa gatatcctaa cagcacaaga gcggaaagat gttttgttct 8700
acatccagaa caacctctgc taaaattcct gaaaaatttt gcaaaaagtt gttgacttta 8760
tctacaaggt gtggtataat aatcttaaca acagcaggac gctcccgggt tgaggaaaac 8820
ctaatgccga gcaaactggc aattagcagc atgagcctgg gtcgttgttt tgcaggtcat 8880
agcctggata gtaaactgga tgcagcacag cgttatggtt atctgggtat tgaactgttt 8940
tatgaggatc tggttgatgt tgcagaacat ctgagcaatg aacgtccgag tccggaaggt 9000
ccgtttgttg aagcacagat tgcagcagca cgtcatattc tgcaaatgtg tcaggcacgt 9060
ggtctggaag ttgtttgtct gcaaccgttt atgcattatg atggtctgaa tgatcgtgcc 9120
gaacatgaac gtcgtctgga aaaactggca ctgtggattg aactggcaca tgaactgcat 9180
accgatatta ttcagattcc ggcaaatttt ctgcctgcaa atcaggttag cgataatctg 9240
gatctgattg ttagcgatct gtgtaaagtt gcagatattg gtgcacaggc actgcctccg 9300
attcgttttg catatgaaag cctgtgttgg agcacccgtg ttgatctgtg ggaacgttgt 9360
tgggatattg ttcagcgtgt ggatcgtccg aattttggta tttgtctgga tacctttaac 9420
atcctgggtc gcatttatgc agatccgacc agcccgagcg gtcgtacccc gaatgcaaaa 9480
gaagcagttc gtaaaagcat tgccaatctg gttagccgtg tggatgttag caaagttttt 9540
tatgttcagg ttgtggatgc cgaacgtctg agtaaaccgc tgctgcctgg tcatccgtat 9600
tataacccgg aacagcctgc acgtatgagc tggtcacgta attgtcgtct gttctatggt 9660
gaaaccgaat atggtgcata tctgccggtt aaagaagttg cacgcgcact gtttcatggt 9720
attggttttg aaggttgggt tagcctggaa ctgtttaatc gtcgtatgag cgaagaaggt 9780
ccggaagttc ctgaagaact ggccatgcgt ggtgcaatta gctgggcaaa actggttcag 9840
gatctgcgta ttccggttga aggtccgctg gttaccatgc ctcgtgttag cgcaagcctg 9900
taaatgcatg cgcgccgcgt tcgcgcggcg cttttttttg gtaccgagct cgaattcact 9960
ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct 10020
tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc 10080
ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac 10140
gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc 10200
cgcatagtta agccagcccc gacacccgcc aacacccgct gacgaattc 10249
<210> 28
<211> 7623
<212> DNA
<213> 质粒pCP32AMP;
<400> 28
acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc 60
tgcaccatta tgttccggat ctgcatcgca ggatgctgct ggctaccctg tggaacacct 120
acatctgtat taacgaagcg ctggcattga ccctgagtga tttttctctg gtcccgccgc 180
atccataccg ccagttgttt accctcacaa cgttccagta accgggcatg ttcatcatca 240
gtaacccgta tcgtgagcat cctctctcgt ttcatcggta tcattacccc catgaacaga 300
aattccccct tacacggagg catcaagtga ccaaacagga aaaaaccgcc cttaacatgg 360
cccgctttat cagaagccag acattaacgc ttctggagaa actcaacgag ctggacgcgg 420
atgaacaggc agacatctgt gaatcgcttc acgaccacgc tgatgagctt taccgcagct 480
gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg 540
tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg 600
gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata 660
ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga 720
aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct 780
cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 840
ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 900
ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 960
cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 1020
actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 1080
cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 1140
tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 1200
gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 1260
caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 1320
agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 1380
tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 1440
tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 1500
gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 1560
gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 1620
aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 1680
atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 1740
gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 1800
acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 1860
ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 1920
tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 1980
ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg 2040
ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 2100
atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 2160
taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 2220
catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 2280
atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc 2340
acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 2400
aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 2460
ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 2520
cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 2580
atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 2640
ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 2700
ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt 2760
tcgtcttcaa gaattctgaa ccagtcctaa aacgagtaaa taggaccggc aattcttcaa 2820
gcaataaaca ggaataccaa ttattaaaag ataacttagt cagatcgtac aataaagctt 2880
tgaagaaaaa tgcgccttat tcaatctttg ctataaaaaa tggcccaaaa tctcacattg 2940
gaagacattt gatgacctca tttctttcaa tgaagggcct aacggagttg actaatgttg 3000
tgggaaattg gagcgataag cgtgcttctg ccgtggccag gacaacgtat actcatcaga 3060
taacagcaat acctgatcac tacttcgcac tagtttctcg gtactatgca tatgatccaa 3120
tatcaaagga aatgatagca ttgaaggatg agactaatcc aattgaggag tggcagcata 3180
tagaacagct aaagggtagt gctgaaggaa gcatacgata ccccgcatgg aatgggataa 3240
tatcacagga ggtactagac tacctttcat cctacataaa tagacgcata taagtacgca 3300
tttaagcata aacacgcact atgccgttct tctcatgtat atatatatac aggcaacacg 3360
cagatatagg tgcgacgtga acagtgagct gtatgtgcgc agctcgcgtt gcattttcgg 3420
aagcgctcgt tttcggaaac gctttgaagt tcctattccg aagttcctat tctctagaaa 3480
gtataggaac ttcagagcgc ttttgaaaac caaaagcgct ctgaagacgc actttcaaaa 3540
aaccaaaaac gcaccggact gtaacgagct actaaaatat tgcgaatacc gcttccacaa 3600
acattgctca aaagtatctc tttgctatat atctctgtgc tatatcccta tataacctac 3660
ccatccacct ttcgctcctt gaacttgcat ctaaactcga cctctacatt ttttatgttt 3720
atctctagta ttactcttta gacaaaaaaa ttgtagtaag aactattcat agagtgaatc 3780
gaaaacaata cgaaaatgta aacatttcct atacgtagta tatagagaca aaatagaaga 3840
aaccgttcat aattttctga ccaatgaaga atcatcaacg ctatcacttt ctgttcacaa 3900
agtatgcgca atccacatcg gtatagaata taatcgggga tgcctttatc ttgaaaaaat 3960
gcacccgcag cttcgctagt aatcagtaaa cgcgggaagt ggagtcaggc tttttttatg 4020
gaagagaaaa tagacaccaa agtagccttc ttctaacctt aacggaccta cagtgcaaaa 4080
agttatcaag agactgcatt atagagcgca caaaggagaa aaaaagtaat ctaagatgct 4140
ttgttagaaa aatagcgctc tcgggatgca tttttgtaga acaaaaaaga agtatagatt 4200
ctttgttggt aaaatagcgc tctcgcgttg catttctgtt ctgtaaaaat gcagctcaga 4260
ttctttgttt gaaaaattag cgctctcgcg ttgcattttt gttttacaaa aatgaagcac 4320
agattcttcg ttggtaaaat agcgctttcg cgttgcattt ctgttctgta aaaatgcagc 4380
tcagattctt tgtttgaaaa attagcgctc tcgcgttgca tttttgttct acaaaatgaa 4440
gcacagatgc ttcgttaaca aagatatgct attgaagtgc aagatggaaa cgcagaaaat 4500
gaaccgggga tgcgacgtgc aagattacct atgcaataga tgcaatagtt tctccaggaa 4560
ccgaaataca tacattgtct tccgtaaagc gctagactat atattattat acaggttcaa 4620
atatactatc tgtttcaggg aaaactccca ggttcggatg ttcaaaattc aatgatgggt 4680
aacaagtacg atcgtaaatc tgtaaaacag tttgtcggat attaggctgt atctcctcaa 4740
agcgtattcg aatatcattg agaagctgca gcgtcacatc ggataataat gatggcagcc 4800
attgtagaag tgccttttgc atttctagtc tctttctcgg tctagctagt tttactacat 4860
cgcgaagata gaatcttaga tcacactgcc tttgctgagc tggatcaata gagtaacaaa 4920
agagtggtaa ggcctcgtta aaggacaagg acctgagcgg aagtgtatcg tacagtagac 4980
ggagtatact agtatagtct atagtccgtg gaattctcat gtttgacagc ttatcatcga 5040
taagcttttc aattcaattc atcatttttt ttttattctt ttttttgatt tcggtttctt 5100
tgaaattttt ttgattcggt aatctccgaa cagaaggaag aacgaaggaa ggagcacaga 5160
cttagattgg tatatatacg catatgtagt gttgaagaaa catgaaattg cccagtattc 5220
ttaacccaac tgcacagaac aaaaacctgc aggaaacgaa gataaatcat gtcgaaagct 5280
acatataagg aacgtgctgc tactcatcct agtcctgttg ctgccaagct atttaatatc 5340
atgcacgaaa agcaaacaaa cttgtgtgct tcattggatg ttcgtaccac caaggaatta 5400
ctggagttag ttgaagcatt aggtcccaaa atttgtttac taaaaacaca tgtggatatc 5460
ttgactgatt tttccatgga gggcacagtt aagccgctaa aggcattatc cgccaagtac 5520
aattttttac tcttcgaaga cagaaaattt gctgacattg gtaatacagt caaattgcag 5580
tactctgcgg gtgtatacag aatagcagaa tgggcagaca ttacgaatgc acacggtgtg 5640
gtgggcccag gtattgttag cggtttgaag caggcggcag aagaagtaac aaaggaacct 5700
agaggccttt tgatgttagc agaattgtca tgcaagggct ccctatctac tggagaatat 5760
actaagggta ctgttgacat tgcgaagagc gacaaagatt ttgttatcgg ctttattgct 5820
caaagagaca tgggtggaag agatgaaggt tacgattggt tgattatgac acccggtgtg 5880
ggtttagatg acaagggaga cgcattgggt caacagtata gaaccgtgga tgatgtggtc 5940
tctacaggat ctgacattat tattgttgga agaggactat ttgcaaaggg aagggatgct 6000
aaggtagagg gtgaacgtta cagaaaagca ggctgggaag catatttgag aagatgcggc 6060
cagcaaaact aaaaaactgt attataagta aatgcatgta tactaaactc acaaattaga 6120
gcttcaattt aattatatca gttattaccc gggaatctcg gtcgtaatga cttgaaataa 6180
ttaacaaaca aaggagttac agttagaaat tgtaggagag atctcgtttt tcgcgacaat 6240
ctggcgtttt tcttgctaat tccaggatta atccgttcat agtgtaaaac cccgtttaca 6300
cattctgacg gaagatatag attggaagta ttgcattcac taagataagt atggcaacac 6360
tggaacagac atgaattatc agaacgacga tttacgcatc aaagaaatca aagagttact 6420
tcctcctgtc gcattgctgg aaaaattccc cgctactgaa aatgccgcga atacggttgc 6480
ccatgcccga aaagcgatcc ataagatcct gaaaggtaat gatgatcgcc tgttggttgt 6540
gattggccca tgctcaattc atgatcctgt cgcggcaaaa gagtatgcca ctcgcttgct 6600
ggcgctgcgt gaagagctga aagatgagct ggaaatcgta atgcgcgtct attttgaaaa 6660
gccgcgtacc acggtgggct ggaaagggct gattaacgat ccgcatatgg ataatagctt 6720
ccagatcaac gacggtctgc gtatagcccg taaattgctg cttgatatta acgacagcgg 6780
tctgccagcg gcaggtgagt ttctcgatat gatcacccca caatatctcg ctgacctgat 6840
gagctggggc gcaattggcg cacgtaccac cgaatcgcag gtgcaccgcg aactggcatc 6900
agggctttct tgtccggtcg gcttcaaaaa tggcaccgac ggtacgatta aagtggctat 6960
cgatgccatt aatgccgccg gtgcgccgca ctgcttcctg tccgtaacga aatgggggca 7020
ttcggcgatt gtgaatacca gcggtaacgg cgattgccat atcattctgc gcggcggtaa 7080
agagcctaac tacagcgcga agcacgttgc tgaagtgaaa gaagggctga acaaagcagg 7140
cctgccagca caggtgatga tcgatttcag ccatgctaac tcgtccaaac aattcaaaaa 7200
gcagatggat gtttgtgctg acgtttgcca gcagattgcc ggtggcgaaa aggccattat 7260
tggcgtgatg gtggaaagcc atctggtgga aggcaatcag agcctcgaga gcggggagcc 7320
gctggcctac ggtaagagca tcaccgatgc ctgcatcggc tgggaagata ccgatgctct 7380
gttacgtcaa ctggcgaatg cagtaaaagc gcgtcgcggg taaggtttaa ttgtcggatg 7440
cgccgtcaga gtggcgtatc cgatgaatca ccacaggcct gataagtcgc gcagcgtcgc 7500
atcaggcaat gtgctccatt gttagcaaca aaaaagccga ctcacttgca gtcggctttc 7560
tcattttaaa cgaatgacgt ttacttcgct ttaccctggt ttgcaaccgc cgctgctttc 7620
gct 7623
<210> 29
<211> 7630
<212> DNA
<213> 质粒pCP14;
<400> 29
ctcgaggcta ttgacgacag ctatggttca ctgtccacca accaaaactg tgctcagtac 60
cgccaatatt tctcccttga ggggtacaaa gaggtgtccc tagaagagat ccacgctgtg 120
taaaaatttt acaaaaaggt attgactttc cctacagggt gtgtaataat ttaattacag 180
gcgggggcaa ccccgcctgt tctagaggag gaggaatcgc catggagagg attgtcgtta 240
ctctcgggga acgtagttac ccaattacca tcgcatctgg tttgtttaat gaaccagctt 300
cattcttacc gctgaaatcg ggcgagcagg tcatgttggt caccaacgaa accctggctc 360
ctctgtatct cgataaggtc cgcggcgtac ttgaacaggc gggtgttaac gtcgatagcg 420
ttatcctccc tgacggcgag cagtataaaa gcctggctgt actcgatacc gtctttacgg 480
cgttgttaca aaagccgcat ggtcgcgata ctacgctggt ggcgcttggc ggcggcgtag 540
tgggcgatct gaccggcttc gcggcggcga gttatcagcg cggtgttcgt ttcattcaag 600
tcccgacgac gttactgtcg caggtcgatt cctccgttgg cggcaaaact gcggtcaacc 660
atcccctcgg taaaaacatg attggcgcgt tctaccagcc tgcttcagtg gtggtggatc 720
tcgactgtct gaaaacgctt cccccgcgtg agttagcgtc ggggctggca gaagtcatca 780
aatacggcat tattcttgac ggtgcgtttt tcaactggct ggaagagaat ctggatgcgt 840
tgttgcgtct ggacggtccg gcaatggcgt actgtattcg ccgttgttgt gaactgaagg 900
cagaagttgt cgccgccgac gagcgcgaaa ccgggttacg tgctttactg aatctgggac 960
acacctttgg tcatgccatt gaagctgaaa tggggtatgg caattggtta catggtgaag 1020
cggtcgctgc gggtatggtg atggcggcgc ggacgtcgga acgtctcggg cagtttagtt 1080
ctgccgaaac gcagcgtatt ataaccctgc tcacgcgggc tgggttaccg gtcaatgggc 1140
cgcgcgaaat gtccgcgcag gcgtatttac cgcatatgct gcgtgacaag aaagtccttg 1200
cgggagagat gcgcttaatt cttccgttgg caattggtaa gagtgaagtt cgcagcggcg 1260
tttcgcacga gcttgttctt aacgccattg ccgattgtca atcagcgtaa tcatcgttca 1320
tgcctgatgc cgctatgtag gccggataag gcgttcacgc cgcatccggc aaccgatgcc 1380
tgatgcgacg cggtcgcgtc ttatcaggcc tacaggtcga tgccgatatg tacatcgtat 1440
tcggcaatta atacatagca acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg 1500
aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca ggatgctgct 1560
ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga ccctgagtga 1620
tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa cgttccagta 1680
accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt ttcatcggta 1740
tcattacccc catgaacaga aattccccct tacacggagg catcaagtga ccaaacagga 1800
aaaaaccgcc cttaacatgg cccgctttat cagaagccag acattaacgc ttctggagaa 1860
actcaacgag ctggacgcgg atgaacaggc agacatctgt gaatcgcttc acgaccacgc 1920
tgatgagctt taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca 1980
catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 2040
ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg 2100
tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga 2160
gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg 2220
cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 2280
gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 2340
aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 2400
gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 2460
aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 2520
gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 2580
ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 2640
cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 2700
ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 2760
actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 2820
tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca 2880
gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 2940
ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 3000
cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 3060
ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 3120
tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 3180
agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 3240
gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 3300
ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 3360
gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 3420
cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 3480
gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 3540
cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 3600
cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 3660
ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 3720
tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 3780
acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 3840
tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 3900
actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 3960
aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 4020
ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 4080
ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 4140
cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat 4200
aggcgtatca cgaggccctt tcgtcttcaa gaattctgaa ccagtcctaa aacgagtaaa 4260
taggaccggc aattcttcaa gcaataaaca ggaataccaa ttattaaaag ataacttagt 4320
cagatcgtac aataaagctt tgaagaaaaa tgcgccttat tcaatctttg ctataaaaaa 4380
tggcccaaaa tctcacattg gaagacattt gatgacctca tttctttcaa tgaagggcct 4440
aacggagttg actaatgttg tgggaaattg gagcgataag cgtgcttctg ccgtggccag 4500
gacaacgtat actcatcaga taacagcaat acctgatcac tacttcgcac tagtttctcg 4560
gtactatgca tatgatccaa tatcaaagga aatgatagca ttgaaggatg agactaatcc 4620
aattgaggag tggcagcata tagaacagct aaagggtagt gctgaaggaa gcatacgata 4680
ccccgcatgg aatgggataa tatcacagga ggtactagac tacctttcat cctacataaa 4740
tagacgcata taagtacgca tttaagcata aacacgcact atgccgttct tctcatgtat 4800
atatatatac aggcaacacg cagatatagg tgcgacgtga acagtgagct gtatgtgcgc 4860
agctcgcgtt gcattttcgg aagcgctcgt tttcggaaac gctttgaagt tcctattccg 4920
aagttcctat tctctagaaa gtataggaac ttcagagcgc ttttgaaaac caaaagcgct 4980
ctgaagacgc actttcaaaa aaccaaaaac gcaccggact gtaacgagct actaaaatat 5040
tgcgaatacc gcttccacaa acattgctca aaagtatctc tttgctatat atctctgtgc 5100
tatatcccta tataacctac ccatccacct ttcgctcctt gaacttgcat ctaaactcga 5160
cctctacatt ttttatgttt atctctagta ttactcttta gacaaaaaaa ttgtagtaag 5220
aactattcat agagtgaatc gaaaacaata cgaaaatgta aacatttcct atacgtagta 5280
tatagagaca aaatagaaga aaccgttcat aattttctga ccaatgaaga atcatcaacg 5340
ctatcacttt ctgttcacaa agtatgcgca atccacatcg gtatagaata taatcgggga 5400
tgcctttatc ttgaaaaaat gcacccgcag cttcgctagt aatcagtaaa cgcgggaagt 5460
ggagtcaggc tttttttatg gaagagaaaa tagacaccaa agtagccttc ttctaacctt 5520
aacggaccta cagtgcaaaa agttatcaag agactgcatt atagagcgca caaaggagaa 5580
aaaaagtaat ctaagatgct ttgttagaaa aatagcgctc tcgggatgca tttttgtaga 5640
acaaaaaaga agtatagatt ctttgttggt aaaatagcgc tctcgcgttg catttctgtt 5700
ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag cgctctcgcg ttgcattttt 5760
gttttacaaa aatgaagcac agattcttcg ttggtaaaat agcgctttcg cgttgcattt 5820
ctgttctgta aaaatgcagc tcagattctt tgtttgaaaa attagcgctc tcgcgttgca 5880
tttttgttct acaaaatgaa gcacagatgc ttcgttaaca aagatatgct attgaagtgc 5940
aagatggaaa cgcagaaaat gaaccgggga tgcgacgtgc aagattacct atgcaataga 6000
tgcaatagtt tctccaggaa ccgaaataca tacattgtct tccgtaaagc gctagactat 6060
atattattat acaggttcaa atatactatc tgtttcaggg aaaactccca ggttcggatg 6120
ttcaaaattc aatgatgggt aacaagtacg atcgtaaatc tgtaaaacag tttgtcggat 6180
attaggctgt atctcctcaa agcgtattcg aatatcattg agaagctgca gcgtcacatc 6240
ggataataat gatggcagcc attgtagaag tgccttttgc atttctagtc tctttctcgg 6300
tctagctagt tttactacat cgcgaagata gaatcttaga tcacactgcc tttgctgagc 6360
tggatcaata gagtaacaaa agagtggtaa ggcctcgtta aaggacaagg acctgagcgg 6420
aagtgtatcg tacagtagac ggagtatact agtatagtct atagtccgtg gaattctcat 6480
gtttgacagc ttatcatcga taagcttttc aattcaattc atcatttttt ttttattctt 6540
ttttttgatt tcggtttctt tgaaattttt ttgattcggt aatctccgaa cagaaggaag 6600
aacgaaggaa ggagcacaga cttagattgg tatatatacg catatgtagt gttgaagaaa 6660
catgaaattg cccagtattc ttaacccaac tgcacagaac aaaaacctgc aggaaacgaa 6720
gataaatcat gtcgaaagct acatataagg aacgtgctgc tactcatcct agtcctgttg 6780
ctgccaagct atttaatatc atgcacgaaa agcaaacaaa cttgtgtgct tcattggatg 6840
ttcgtaccac caaggaatta ctggagttag ttgaagcatt aggtcccaaa atttgtttac 6900
taaaaacaca tgtggatatc ttgactgatt tttccatgga gggcacagtt aagccgctaa 6960
aggcattatc cgccaagtac aattttttac tcttcgaaga cagaaaattt gctgacattg 7020
gtaatacagt caaattgcag tactctgcgg gtgtatacag aatagcagaa tgggcagaca 7080
ttacgaatgc acacggtgtg gtgggcccag gtattgttag cggtttgaag caggcggcag 7140
aagaagtaac aaaggaacct agaggccttt tgatgttagc agaattgtca tgcaagggct 7200
ccctatctac tggagaatat actaagggta ctgttgacat tgcgaagagc gacaaagatt 7260
ttgttatcgg ctttattgct caaagagaca tgggtggaag agatgaaggt tacgattggt 7320
tgattatgac acccggtgtg ggtttagatg acaagggaga cgcattgggt caacagtata 7380
gaaccgtgga tgatgtggtc tctacaggat ctgacattat tattgttgga agaggactat 7440
ttgcaaaggg aagggatgct aaggtagagg gtgaacgtta cagaaaagca ggctgggaag 7500
catatttgag aagatgcggc cagcaaaact aaaaaactgt attataagta aatgcatgta 7560
tactaaactc acaaattaga gcttcaattt aattatatca gttattaccc gggaatctcg 7620
gtcgtaatga 7630
<210> 30
<211> 10015
<212> DNA
<213> 质粒pCP50;
<400> 30
cttgaaataa ttaacaaaca aaggagttac agttagaaat tgtaggagag atctcgtttt 60
tcgcgacaat ctggcgtttt tcttgctaat tccaggatta atccgttcat agtgtaaaac 120
cccgtttaca cattctgacg gaagatatag attggaagta ttgcattcac taagataagt 180
atggcaacac tggaacagac atgaattatc agaacgacga tttacgcatc aaagaaatca 240
aagagttact tcctcctgtc gcattgctgg aaaaattccc cgctactgaa aatgccgcga 300
atacggttgc ccatgcccga aaagcgatcc ataagatcct gaaaggtaat gatgatcgcc 360
tgttggttgt gattggccca tgctcaattc atgatcctgt cgcggcaaaa gagtatgcca 420
ctcgcttgct ggcgctgcgt gaagagctga aagatgagct ggaaatcgta atgcgcgtct 480
attttgaaaa gccgcgtacc acggtgggct ggaaagggct gattaacgat ccgcatatgg 540
ataatagctt ccagatcaac gacggtctgc gtatagcccg taaattgctg cttgatatta 600
acgacagcgg tctgccagcg gcaggtgagt ttctcgatat gatcacccca caatatctcg 660
ctgacctgat gagctggggc gcaattggcg cacgtaccac cgaatcgcag gtgcaccgcg 720
aactggcatc agggctttct tgtccggtcg gcttcaaaaa tggcaccgac ggtacgatta 780
aagtggctat cgatgccatt aatgccgccg gtgcgccgca ctgcttcctg tccgtaacga 840
aatgggggca ttcggcgatt gtgaatacca gcggtaacgg cgattgccat atcattctgc 900
gcggcggtaa agagcctaac tacagcgcga agcacgttgc tgaagtgaaa gaagggctga 960
acaaagcagg cctgccagca caggtgatga tcgatttcag ccatgctaac tcgtccaaac 1020
aattcaaaaa gcagatggat gtttgtgctg acgtttgcca gcagattgcc ggtggcgaaa 1080
aggccattat tggcgtgatg gtggaaagcc atctggtgga aggcaatcag agcctcgaga 1140
gcggggagcc gctggcctac ggtaagagca tcaccgatgc ctgcatcggc tgggaagata 1200
ccgatgctct gttacgtcaa ctggcgaatg cagtaaaagc gcgtcgcggg taaggtttaa 1260
ttgtcggatg cgccgtcaga gtggcgtatc cgatgaatca ccacaggcct gataagtcgc 1320
gcagcgtcgc atcaggcaat gtgctccatt gttagcaaca aaaaagccga ctcacttgca 1380
gtcggctttc tcattttaaa cgaatgacgt ttacttcgct ttaccctggt ttgcaaccgc 1440
cgctgctttc gctacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg 1500
gaagtcagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct gctggctacc 1560
ctgtggaaca cctacatctg tattaacgaa gcgctggcat tgaccctgag tgatttttct 1620
ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca gtaaccgggc 1680
atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg gtatcattac 1740
ccccatgaac agaaattccc ccttacacgg aggcatcaag tgaccaaaca ggaaaaaacc 1800
gcccttaaca tggcccgctt tatcagaagc cagacattaa cgcttctgga gaaactcaac 1860
gagctggacg cggatgaaca ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag 1920
ctttaccgca gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag 1980
ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag 2040
ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat 2100
agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc 2160
atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt 2220
ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 2280
ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 2340
tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 2400
tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 2460
gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 2520
ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 2580
tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 2640
agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 2700
atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 2760
acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 2820
actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 2880
tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 2940
tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 3000
tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 3060
tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 3120
caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 3180
cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 3240
agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 3300
acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 3360
gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 3420
ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca 3480
tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 3540
ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 3600
tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 3660
attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 3720
agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg 3780
ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 3840
ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 3900
cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 3960
gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 4020
tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 4080
tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 4140
tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta 4200
tcacgaggcc ctttcgtctt caagaattct gaaccagtcc taaaacgagt aaataggacc 4260
ggcaattctt caagcaataa acaggaatac caattattaa aagataactt agtcagatcg 4320
tacaataaag ctttgaagaa aaatgcgcct tattcaatct ttgctataaa aaatggccca 4380
aaatctcaca ttggaagaca tttgatgacc tcatttcttt caatgaaggg cctaacggag 4440
ttgactaatg ttgtgggaaa ttggagcgat aagcgtgctt ctgccgtggc caggacaacg 4500
tatactcatc agataacagc aatacctgat cactacttcg cactagtttc tcggtactat 4560
gcatatgatc caatatcaaa ggaaatgata gcattgaagg atgagactaa tccaattgag 4620
gagtggcagc atatagaaca gctaaagggt agtgctgaag gaagcatacg ataccccgca 4680
tggaatggga taatatcaca ggaggtacta gactaccttt catcctacat aaatagacgc 4740
atataagtac gcatttaagc ataaacacgc actatgccgt tcttctcatg tatatatata 4800
tacaggcaac acgcagatat aggtgcgacg tgaacagtga gctgtatgtg cgcagctcgc 4860
gttgcatttt cggaagcgct cgttttcgga aacgctttga agttcctatt ccgaagttcc 4920
tattctctag aaagtatagg aacttcagag cgcttttgaa aaccaaaagc gctctgaaga 4980
cgcactttca aaaaaccaaa aacgcaccgg actgtaacga gctactaaaa tattgcgaat 5040
accgcttcca caaacattgc tcaaaagtat ctctttgcta tatatctctg tgctatatcc 5100
ctatataacc tacccatcca cctttcgctc cttgaacttg catctaaact cgacctctac 5160
attttttatg tttatctcta gtattactct ttagacaaaa aaattgtagt aagaactatt 5220
catagagtga atcgaaaaca atacgaaaat gtaaacattt cctatacgta gtatatagag 5280
acaaaataga agaaaccgtt cataattttc tgaccaatga agaatcatca acgctatcac 5340
tttctgttca caaagtatgc gcaatccaca tcggtataga atataatcgg ggatgccttt 5400
atcttgaaaa aatgcacccg cagcttcgct agtaatcagt aaacgcggga agtggagtca 5460
ggcttttttt atggaagaga aaatagacac caaagtagcc ttcttctaac cttaacggac 5520
ctacagtgca aaaagttatc aagagactgc attatagagc gcacaaagga gaaaaaaagt 5580
aatctaagat gctttgttag aaaaatagcg ctctcgggat gcatttttgt agaacaaaaa 5640
agaagtatag attctttgtt ggtaaaatag cgctctcgcg ttgcatttct gttctgtaaa 5700
aatgcagctc agattctttg tttgaaaaat tagcgctctc gcgttgcatt tttgttttac 5760
aaaaatgaag cacagattct tcgttggtaa aatagcgctt tcgcgttgca tttctgttct 5820
gtaaaaatgc agctcagatt ctttgtttga aaaattagcg ctctcgcgtt gcatttttgt 5880
tctacaaaat gaagcacaga tgcttcgtta acaaagatat gctattgaag tgcaagatgg 5940
aaacgcagaa aatgaaccgg ggatgcgacg tgcaagatta cctatgcaat agatgcaata 6000
gtttctccag gaaccgaaat acatacattg tcttccgtaa agcgctagac tatatattat 6060
tatacaggtt caaatatact atctgtttca gggaaaactc ccaggttcgg atgttcaaaa 6120
ttcaatgatg ggtaacaagt acgatcgtaa atctgtaaaa cagtttgtcg gatattaggc 6180
tgtatctcct caaagcgtat tcgaatatca ttgagaagct gcagcgtcac atcggataat 6240
aatgatggca gccattgtag aagtgccttt tgcatttcta gtctctttct cggtctagct 6300
agttttacta catcgcgaag atagaatctt agatcacact gcctttgctg agctggatca 6360
atagagtaac aaaagagtgg taaggcctcg ttaaaggaca aggacctgag cggaagtgta 6420
tcgtacagta gacggagtat actagtatag tctatagtcc gtggaattct catgtttgac 6480
agcttatcat cgataagctt ttcaattcaa ttcatcattt tttttttatt cttttttttg 6540
atttcggttt ctttgaaatt tttttgattc ggtaatctcc gaacagaagg aagaacgaag 6600
gaaggagcac agacttagat tggtatatat acgcatatgt agtgttgaag aaacatgaaa 6660
ttgcccagta ttcttaaccc aactgcacag aacaaaaacc tgcaggaaac gaagataaat 6720
catgtcgaaa gctacatata aggaacgtgc tgctactcat cctagtcctg ttgctgccaa 6780
gctatttaat atcatgcacg aaaagcaaac aaacttgtgt gcttcattgg atgttcgtac 6840
caccaaggaa ttactggagt tagttgaagc attaggtccc aaaatttgtt tactaaaaac 6900
acatgtggat atcttgactg atttttccat ggagggcaca gttaagccgc taaaggcatt 6960
atccgccaag tacaattttt tactcttcga agacagaaaa tttgctgaca ttggtaatac 7020
agtcaaattg cagtactctg cgggtgtata cagaatagca gaatgggcag acattacgaa 7080
tgcacacggt gtggtgggcc caggtattgt tagcggtttg aagcaggcgg cagaagaagt 7140
aacaaaggaa cctagaggcc ttttgatgtt agcagaattg tcatgcaagg gctccctatc 7200
tactggagaa tatactaagg gtactgttga cattgcgaag agcgacaaag attttgttat 7260
cggctttatt gctcaaagag acatgggtgg aagagatgaa ggttacgatt ggttgattat 7320
gacacccggt gtgggtttag atgacaaggg agacgcattg ggtcaacagt atagaaccgt 7380
ggatgatgtg gtctctacag gatctgacat tattattgtt ggaagaggac tatttgcaaa 7440
gggaagggat gctaaggtag agggtgaacg ttacagaaaa gcaggctggg aagcatattt 7500
gagaagatgc ggccagcaaa actaaaaaac tgtattataa gtaaatgcat gtatactaaa 7560
ctcacaaatt agagcttcaa tttaattata tcagttatta cccgggaatc tcggtcgtaa 7620
tgaaaggaaa agcgcaacgg acgggcgagt agattgcgca acatgcgagc atgatccaga 7680
gatttctgaa gcagcaaaag gatgttccat gtacatgacg cgcggcttgc ggtaaattgt 7740
tggcaaattt tccggcgtag cccaaaacgc gctgtcgtca agtcgttaag ggcgtgccct 7800
tcatcatccg atctggagtc aaaatgtcct cacgtaaaga gcttgccaat gctattcgtg 7860
cgctgagcat ggacgcagta cagaaagcca aatccggtca cccgggtgcc cctatgggta 7920
tggctgacat tgccgaagtc ctgtggcgtg atttcctgaa acacaacccg cagaatccgt 7980
cctgggctga ccgtgaccgc ttcgtgctgt ccaacggcca cggctccatg ctgatctaca 8040
gcctgctgca cctcaccggt tacgatctgc cgatggaaga actgaaaaac ttccgtcagc 8100
tgcactctaa aactccgggc cacccggaag taggttatac cgctggtgtg gaaaccacca 8160
ccggtccgct gggtcagggt attgccaacg cagtcggtat ggcgattgca gaaaaaacgc 8220
tggcggcgca gtttaaccgt ccaggtcacg acattgtcga ccactacacc tacgccttca 8280
tgggcgacgg ctgcatgatg gaaggcatct cccacgaagt ttgctctctg gcgggtacgc 8340
tgaagctggg taaactgatt gcgttctacg atgacaacgg tatctcaatc gatggtcacg 8400
ttgaaggctg gttcactgac gacaccgcaa tgcgtttcga agcttacggc tggcacgtta 8460
ttcgcgacat cgacggtcat gacgcggcat ccatcaaacg cgcagtagaa gaagcgcgcg 8520
cagtgactga caaaccgtcc ctgctgatgt gcaaaaccat catcggtttc ggttccccga 8580
acaaagccgg tacccacgac tcccacggtg cgccgctggg cgacgctgaa attgccctga 8640
cccgcgaaca gctgggctgg aaatacgcgc cgttcgaaat cccgtctgaa atctatgctc 8700
agtgggatgc gaaagaagca ggccaggcga aagaatctgc atggaatgag aagtttgcgg 8760
cttacgcgaa agcttatccg caggaagcgg ctgaatttac ccgccgtatg aaaggcgaaa 8820
tgccgtctga cttcgacgcc aaagcgaaag agtttatcgc taaactgcag gctaatccgg 8880
cgaaaatcgc cagccgtaaa gcgtcgcaga atgctatcga agcgttcggc ccgctgttgc 8940
ctgaattcct cggcggctct gctgacctgg caccgtctaa cctgaccctg tggtctggtt 9000
ctaaagcaat caacgaagat gctgcaggta actacatcca ctacggtgtt cgcgagttcg 9060
gtatgaccgc gattgctaac ggtatctccc tgcacggtgg tttcctgccg tacacctcca 9120
ccttcctgat gttcgtggaa tacgcacgta acgccgtacg tatggctgcg ctgatgaaac 9180
agcgtcaggt gatggtttac acccacgact ccatcggtct gggcgaagat ggcccgactc 9240
accagccggt tgagcaggtc gcttctctgc gcgtgacccc gaacatgtct acatggcgtc 9300
cgtgtgacca ggttgaatcc gcggtcgcgt ggaaatacgg cgttgagcgt caggacggcc 9360
cgactgcgct tatcctctcc cgtcagaacc tggcgcagca ggaacgaact gaagagcaac 9420
tggcaaacat cgcgcgcggt ggttatgtgc tgaaagactg cgccggtcag ccggaactga 9480
ttttcatcgc taccggttca gaagttgaac tggctgttgc tgcctacgaa aaactgactg 9540
ccgaaggcgt gaaagcgcgc gtggtgtcca tgccgtctac cgacgcattt gacaagcagg 9600
atgctgctta ccgtgaatcc gtactgccga aagcggttac tgcacgcgtt gctgtagaag 9660
cgggtattgc tgactactgg tacaagtatg ttggcctgaa cggtgctatc gtcggtatga 9720
ccaccttcgg tgaatctgct ccggcagagc tgctgtttga agagttcggc ttcactgttg 9780
ataacgttgt tgcgaaagca aaagaactgc tgtaattagc atttcgggta aaaaggtcgc 9840
ttcggcgacc ttttttatta ccttgatatg tccgtttgcg gacaagcaat agataaagcg 9900
tgttgtagat cacaaatatt tatatgcaat aaatatcaat tatgtaatat gcatcacgat 9960
atgcgtattg acatttgttg ttataactat aactcaatgt tatataagaa attaa 10015
<210> 31
<211> 9065
<212> DNA
<213> 质粒pCP54;
<400> 31
acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc 60
tgcaccatta tgttccggat ctgcatcgca ggatgctgct ggctaccctg tggaacacct 120
acatctgtat taacgaagcg ctggcattga ccctgagtga tttttctctg gtcccgccgc 180
atccataccg ccagttgttt accctcacaa cgttccagta accgggcatg ttcatcatca 240
gtaacccgta tcgtgagcat cctctctcgt ttcatcggta tcattacccc catgaacaga 300
aattccccct tacacggagg catcaagtga ccaaacagga aaaaaccgcc cttaacatgg 360
cccgctttat cagaagccag acattaacgc ttctggagaa actcaacgag ctggacgcgg 420
atgaacaggc agacatctgt gaatcgcttc acgaccacgc tgatgagctt taccgcagct 480
gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg 540
tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg 600
gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata 660
ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga 720
aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct 780
cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 840
ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 900
ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 960
cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 1020
actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 1080
cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 1140
tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 1200
gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 1260
caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 1320
agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 1380
tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 1440
tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 1500
gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 1560
gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 1620
aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 1680
atatgagtaa acttggtctg agtggcggtt ttcatggctt gttatgactg tttttttggg 1740
gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg 1800
atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac 1860
atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc 1920
atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat 1980
ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat 2040
gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag 2100
agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg 2160
cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca 2220
ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga 2280
gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa 2340
caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg 2400
gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc 2460
ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag 2520
tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg 2580
gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag 2640
gtagtcggca aataatgtct aacaattcgt tcaagccgac gccgcttcgc ggcgcggctt 2700
aactcaagcg ttagatgcac taagcacata attgctcaca gccaaactat cagaattctg 2760
aaccagtcct aaaacgagta aataggaccg gcaattcttc aagcaataaa caggaatacc 2820
aattattaaa agataactta gtcagatcgt acaataaagc tttgaagaaa aatgcgcctt 2880
attcaatctt tgctataaaa aatggcccaa aatctcacat tggaagacat ttgatgacct 2940
catttctttc aatgaagggc ctaacggagt tgactaatgt tgtgggaaat tggagcgata 3000
agcgtgcttc tgccgtggcc aggacaacgt atactcatca gataacagca atacctgatc 3060
actacttcgc actagtttct cggtactatg catatgatcc aatatcaaag gaaatgatag 3120
cattgaagga tgagactaat ccaattgagg agtggcagca tatagaacag ctaaagggta 3180
gtgctgaagg aagcatacga taccccgcat ggaatgggat aatatcacag gaggtactag 3240
actacctttc atcctacata aatagacgca tataagtacg catttaagca taaacacgca 3300
ctatgccgtt cttctcatgt atatatatat acaggcaaca cgcagatata ggtgcgacgt 3360
gaacagtgag ctgtatgtgc gcagctcgcg ttgcattttc ggaagcgctc gttttcggaa 3420
acgctttgaa gttcctattc cgaagttcct attctctaga aagtatagga acttcagagc 3480
gcttttgaaa accaaaagcg ctctgaagac gcactttcaa aaaaccaaaa acgcaccgga 3540
ctgtaacgag ctactaaaat attgcgaata ccgcttccac aaacattgct caaaagtatc 3600
tctttgctat atatctctgt gctatatccc tatataacct acccatccac ctttcgctcc 3660
ttgaacttgc atctaaactc gacctctaca ttttttatgt ttatctctag tattactctt 3720
tagacaaaaa aattgtagta agaactattc atagagtgaa tcgaaaacaa tacgaaaatg 3780
taaacatttc ctatacgtag tatatagaga caaaatagaa gaaaccgttc ataattttct 3840
gaccaatgaa gaatcatcaa cgctatcact ttctgttcac aaagtatgcg caatccacat 3900
cggtatagaa tataatcggg gatgccttta tcttgaaaaa atgcacccgc agcttcgcta 3960
gtaatcagta aacgcgggaa gtggagtcag gcttttttta tggaagagaa aatagacacc 4020
aaagtagcct tcttctaacc ttaacggacc tacagtgcaa aaagttatca agagactgca 4080
ttatagagcg cacaaaggag aaaaaaagta atctaagatg ctttgttaga aaaatagcgc 4140
tctcgggatg catttttgta gaacaaaaaa gaagtataga ttctttgttg gtaaaatagc 4200
gctctcgcgt tgcatttctg ttctgtaaaa atgcagctca gattctttgt ttgaaaaatt 4260
agcgctctcg cgttgcattt ttgttttaca aaaatgaagc acagattctt cgttggtaaa 4320
atagcgcttt cgcgttgcat ttctgttctg taaaaatgca gctcagattc tttgtttgaa 4380
aaattagcgc tctcgcgttg catttttgtt ctacaaaatg aagcacagat gcttcgttaa 4440
caaagatatg ctattgaagt gcaagatgga aacgcagaaa atgaaccggg gatgcgacgt 4500
gcaagattac ctatgcaata gatgcaatag tttctccagg aaccgaaata catacattgt 4560
cttccgtaaa gcgctagact atatattatt atacaggttc aaatatacta tctgtttcag 4620
ggaaaactcc caggttcgga tgttcaaaat tcaatgatgg gtaacaagta cgatcgtaaa 4680
tctgtaaaac agtttgtcgg atattaggct gtatctcctc aaagcgtatt cgaatatcat 4740
tgagaagctg cagcgtcaca tcggataata atgatggcag ccattgtaga agtgcctttt 4800
gcatttctag tctctttctc ggtctagcta gttttactac atcgcgaaga tagaatctta 4860
gatcacactg cctttgctga gctggatcaa tagagtaaca aaagagtggt aaggcctcgt 4920
taaaggacaa ggacctgagc ggaagtgtat cgtacagtag acggagtata ctagtatagt 4980
ctatagtccg tggaattctc atgtttgaca gcttatcatc gataagcttt tcaattcaat 5040
tcatcatttt ttttttattc ttttttttga tttcggtttc tttgaaattt ttttgattcg 5100
gtaatctccg aacagaagga agaacgaagg aaggagcaca gacttagatt ggtatatata 5160
cgcatatgta gtgttgaaga aacatgaaat tgcccagtat tcttaaccca actgcacaga 5220
acaaaaacct gcaggaaacg aagataaatc atgtcgaaag ctacatataa ggaacgtgct 5280
gctactcatc ctagtcctgt tgctgccaag ctatttaata tcatgcacga aaagcaaaca 5340
aacttgtgtg cttcattgga tgttcgtacc accaaggaat tactggagtt agttgaagca 5400
ttaggtccca aaatttgttt actaaaaaca catgtggata tcttgactga tttttccatg 5460
gagggcacag ttaagccgct aaaggcatta tccgccaagt acaatttttt actcttcgaa 5520
gacagaaaat ttgctgacat tggtaataca gtcaaattgc agtactctgc gggtgtatac 5580
agaatagcag aatgggcaga cattacgaat gcacacggtg tggtgggccc aggtattgtt 5640
agcggtttga agcaggcggc agaagaagta acaaaggaac ctagaggcct tttgatgtta 5700
gcagaattgt catgcaaggg ctccctatct actggagaat atactaaggg tactgttgac 5760
attgcgaaga gcgacaaaga ttttgttatc ggctttattg ctcaaagaga catgggtgga 5820
agagatgaag gttacgattg gttgattatg acacccggtg tgggtttaga tgacaaggga 5880
gacgcattgg gtcaacagta tagaaccgtg gatgatgtgg tctctacagg atctgacatt 5940
attattgttg gaagaggact atttgcaaag ggaagggatg ctaaggtaga gggtgaacgt 6000
tacagaaaag caggctggga agcatatttg agaagatgcg gccagcaaaa ctaaaaaact 6060
gtattataag taaatgcatg tatactaaac tcacaaatta gagcttcaat ttaattatat 6120
cagttattac ccgggaatct cggtcgtaat gacttgaaat aattaacaaa caaaggagtt 6180
acagttagaa attgtaggag agatctcgtt tttcgcgaca atctggcgtt tttcttgcta 6240
attccaggat taatccgttc atagtgtaaa accccgttta cacattctga cggaagatat 6300
agattggaag tattgcattc actaagataa gtatggcaac actggaacag acatgaatta 6360
tcagaacgac gatttacgca tcaaagaaat caaagagtta cttcctcctg tcgcattgct 6420
ggaaaaattc cccgctactg aaaatgccgc gaatacggtt gcccatgccc gaaaagcgat 6480
ccataagatc ctgaaaggta atgatgatcg cctgttggtt gtgattggcc catgctcaat 6540
tcatgatcct gtcgcggcaa aagagtatgc cactcgcttg ctggcgctgc gtgaagagct 6600
gaaagatgag ctggaaatcg taatgcgcgt ctattttgaa aagccgcgta ccacggtggg 6660
ctggaaaggg ctgattaacg atccgcatat ggataatagc ttccagatca acgacggtct 6720
gcgtatagcc cgtaaattgc tgcttgatat taacgacagc ggtctgccag cggcaggtga 6780
gtttctcgat atgatcaccc cacaatatct cgctgacctg atgagctggg gcgcaattgg 6840
cgcacgtacc accgaatcgc aggtgcaccg cgaactggca tcagggcttt cttgtccggt 6900
cggcttcaaa aatggcaccg acggtacgat taaagtggct atcgatgcca ttaatgccgc 6960
cggtgcgccg cactgcttcc tgtccgtaac gaaatggggg cattcggcga ttgtgaatac 7020
cagcggtaac ggcgattgcc atatcattct gcgcggcggt aaagagccta actacagcgc 7080
gaagcacgtt gctgaagtga aagaagggct gaacaaagca ggcctgccag cacaggtgat 7140
gatcgatttc agccatgcta actcgtccaa acaattcaaa aagcagatgg atgtttgtgc 7200
tgacgtttgc cagcagattg ccggtggcga aaaggccatt attggcgtga tggtggaaag 7260
ccatctggtg gaaggcaatc agagcctcga gagcggggag ccgctggcct acggtaagag 7320
catcaccgat gcctgcatcg gctgggaaga taccgatgct ctgttacgtc aactggcgaa 7380
tgcagtaaaa gcgcgtcgcg ggtaaggttt aattgtcgga tgcgccgtca gagtggcgta 7440
tccgatgaat caccacaggc ctgataagtc gcgcagcgtc gcatcaggca atgtgctcca 7500
ttgttagcaa caaaaaagcc gactcacttg cagtcggctt tctcatttta aacgaatgac 7560
gtttacttcg ctttaccctg gtttgcaacc gccgctgctt tcgctctcga ggctattgac 7620
gacagctatg gttcactgtc caccaaccaa aactgtgctc agtaccgcca atatttctcc 7680
cttgaggggt acaaagaggt gtccctagaa gagatccacg ctgtgtaaaa attttacaaa 7740
aaggtattga ctttccctac agggtgtgta ataatttaat tacaggcggg ggcaaccccg 7800
cctgttctag aggaggagga atcgccatgg agaggattgt cgttactctc ggggaacgta 7860
gttacccaat taccatcgca tctggtttgt ttaatgaacc agcttcattc ttaccgctga 7920
aatcgggcga gcaggtcatg ttggtcacca acgaaaccct ggctcctctg tatctcgata 7980
aggtccgcgg cgtacttgaa caggcgggtg ttaacgtcga tagcgttatc ctccctgacg 8040
gcgagcagta taaaagcctg gctgtactcg ataccgtctt tacggcgttg ttacaaaagc 8100
cgcatggtcg cgatactacg ctggtggcgc ttggcggcgg cgtagtgggc gatctgaccg 8160
gcttcgcggc ggcgagttat cagcgcggtg ttcgtttcat tcaagtcccg acgacgttac 8220
tgtcgcaggt cgattcctcc gttggcggca aaactgcggt caaccatccc ctcggtaaaa 8280
acatgattgg cgcgttctac cagcctgctt cagtggtggt ggatctcgac tgtctgaaaa 8340
cgcttccccc gcgtgagtta gcgtcggggc tggcagaagt catcaaatac ggcattattc 8400
ttgacggtgc gtttttcaac tggctggaag agaatctgga tgcgttgttg cgtctggacg 8460
gtccggcaat ggcgtactgt attcgccgtt gttgtgaact gaaggcagaa gttgtcgccg 8520
ccgacgagcg cgaaaccggg ttacgtgctt tactgaatct gggacacacc tttggtcatg 8580
ccattgaagc tgaaatgggg tatggcaatt ggttacatgg tgaagcggtc gctgcgggta 8640
tggtgatggc ggcgcggacg tcggaacgtc tcgggcagtt tagttctgcc gaaacgcagc 8700
gtattataac cctgctcacg cgggctgggt taccggtcaa tgggccgcgc gaaatgtccg 8760
cgcaggcgta tttaccgcat atgctgcgtg acaagaaagt ccttgcggga gagatgcgct 8820
taattcttcc gttggcaatt ggtaagagtg aagttcgcag cggcgtttcg cacgagcttg 8880
ttcttaacgc cattgccgat tgtcaatcag cgtaatcatc gttcatgcct gatgccgcta 8940
tgtaggccgg ataaggcgtt cacgccgcat ccggcaaccg atgcctgatg cgacgcggtc 9000
gcgtcttatc aggcctacag gtcgatgccg atatgtacat cgtattcggc aattaataca 9060
tagca 9065
<210> 32
<211> 11475
<212> DNA
<213> 质粒pCP55;
<400> 32
acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc 60
tgcaccatta tgttccggat ctgcatcgca ggatgctgct ggctaccctg tggaacacct 120
acatctgtat taacgaagcg ctggcattga ccctgagtga tttttctctg gtcccgccgc 180
atccataccg ccagttgttt accctcacaa cgttccagta accgggcatg ttcatcatca 240
gtaacccgta tcgtgagcat cctctctcgt ttcatcggta tcattacccc catgaacaga 300
aattccccct tacacggagg catcaagtga ccaaacagga aaaaaccgcc cttaacatgg 360
cccgctttat cagaagccag acattaacgc ttctggagaa actcaacgag ctggacgcgg 420
atgaacaggc agacatctgt gaatcgcttc acgaccacgc tgatgagctt taccgcagct 480
gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg 540
tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg 600
gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata 660
ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga 720
aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct 780
cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 840
ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 900
ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 960
cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 1020
actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 1080
cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 1140
tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 1200
gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 1260
caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 1320
agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 1380
tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 1440
tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 1500
gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 1560
gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 1620
aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 1680
atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 1740
gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 1800
acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 1860
ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 1920
tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 1980
ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg 2040
ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 2100
atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 2160
taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 2220
catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 2280
atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc 2340
acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 2400
aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 2460
ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 2520
cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 2580
atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 2640
ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 2700
ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt 2760
tcgtcttcaa gaattctgaa ccagtcctaa aacgagtaaa taggaccggc aattcttcaa 2820
gcaataaaca ggaataccaa ttattaaaag ataacttagt cagatcgtac aataaagctt 2880
tgaagaaaaa tgcgccttat tcaatctttg ctataaaaaa tggcccaaaa tctcacattg 2940
gaagacattt gatgacctca tttctttcaa tgaagggcct aacggagttg actaatgttg 3000
tgggaaattg gagcgataag cgtgcttctg ccgtggccag gacaacgtat actcatcaga 3060
taacagcaat acctgatcac tacttcgcac tagtttctcg gtactatgca tatgatccaa 3120
tatcaaagga aatgatagca ttgaaggatg agactaatcc aattgaggag tggcagcata 3180
tagaacagct aaagggtagt gctgaaggaa gcatacgata ccccgcatgg aatgggataa 3240
tatcacagga ggtactagac tacctttcat cctacataaa tagacgcata taagtacgca 3300
tttaagcata aacacgcact atgccgttct tctcatgtat atatatatac aggcaacacg 3360
cagatatagg tgcgacgtga acagtgagct gtatgtgcgc agctcgcgtt gcattttcgg 3420
aagcgctcgt tttcggaaac gctttgaagt tcctattccg aagttcctat tctctagaaa 3480
gtataggaac ttcagagcgc ttttgaaaac caaaagcgct ctgaagacgc actttcaaaa 3540
aaccaaaaac gcaccggact gtaacgagct actaaaatat tgcgaatacc gcttccacaa 3600
acattgctca aaagtatctc tttgctatat atctctgtgc tatatcccta tataacctac 3660
ccatccacct ttcgctcctt gaacttgcat ctaaactcga cctctacatt ttttatgttt 3720
atctctagta ttactcttta gacaaaaaaa ttgtagtaag aactattcat agagtgaatc 3780
gaaaacaata cgaaaatgta aacatttcct atacgtagta tatagagaca aaatagaaga 3840
aaccgttcat aattttctga ccaatgaaga atcatcaacg ctatcacttt ctgttcacaa 3900
agtatgcgca atccacatcg gtatagaata taatcgggga tgcctttatc ttgaaaaaat 3960
gcacccgcag cttcgctagt aatcagtaaa cgcgggaagt ggagtcaggc tttttttatg 4020
gaagagaaaa tagacaccaa agtagccttc ttctaacctt aacggaccta cagtgcaaaa 4080
agttatcaag agactgcatt atagagcgca caaaggagaa aaaaagtaat ctaagatgct 4140
ttgttagaaa aatagcgctc tcgggatgca tttttgtaga acaaaaaaga agtatagatt 4200
ctttgttggt aaaatagcgc tctcgcgttg catttctgtt ctgtaaaaat gcagctcaga 4260
ttctttgttt gaaaaattag cgctctcgcg ttgcattttt gttttacaaa aatgaagcac 4320
agattcttcg ttggtaaaat agcgctttcg cgttgcattt ctgttctgta aaaatgcagc 4380
tcagattctt tgtttgaaaa attagcgctc tcgcgttgca tttttgttct acaaaatgaa 4440
gcacagatgc ttcgttaaca aagatatgct attgaagtgc aagatggaaa cgcagaaaat 4500
gaaccgggga tgcgacgtgc aagattacct atgcaataga tgcaatagtt tctccaggaa 4560
ccgaaataca tacattgtct tccgtaaagc gctagactat atattattat acaggttcaa 4620
atatactatc tgtttcaggg aaaactccca ggttcggatg ttcaaaattc aatgatgggt 4680
aacaagtacg atcgtaaatc tgtaaaacag tttgtcggat attaggctgt atctcctcaa 4740
agcgtattcg aatatcattg agaagctgca gcgtcacatc ggataataat gatggcagcc 4800
attgtagaag tgccttttgc atttctagtc tctttctcgg tctagctagt tttactacat 4860
cgcgaagata gaatcttaga tcacactgcc tttgctgagc tggatcaata gagtaacaaa 4920
agagtggtaa ggcctcgtta aaggacaagg acctgagcgg aagtgtatcg tacagtagac 4980
ggagtatact agtatagtct atagtccgtg gaattctcat gtttgacagc ttatcatcga 5040
taagcttttc aattcaattc atcatttttt ttttattctt ttttttgatt tcggtttctt 5100
tgaaattttt ttgattcggt aatctccgaa cagaaggaag aacgaaggaa ggagcacaga 5160
cttagattgg tatatatacg catatgtagt gttgaagaaa catgaaattg cccagtattc 5220
ttaacccaac tgcacagaac aaaaacctgc aggaaacgaa gataaatcat gtcgaaagct 5280
acatataagg aacgtgctgc tactcatcct agtcctgttg ctgccaagct atttaatatc 5340
atgcacgaaa agcaaacaaa cttgtgtgct tcattggatg ttcgtaccac caaggaatta 5400
ctggagttag ttgaagcatt aggtcccaaa atttgtttac taaaaacaca tgtggatatc 5460
ttgactgatt tttccatgga gggcacagtt aagccgctaa aggcattatc cgccaagtac 5520
aattttttac tcttcgaaga cagaaaattt gctgacattg gtaatacagt caaattgcag 5580
tactctgcgg gtgtatacag aatagcagaa tgggcagaca ttacgaatgc acacggtgtg 5640
gtgggcccag gtattgttag cggtttgaag caggcggcag aagaagtaac aaaggaacct 5700
agaggccttt tgatgttagc agaattgtca tgcaagggct ccctatctac tggagaatat 5760
actaagggta ctgttgacat tgcgaagagc gacaaagatt ttgttatcgg ctttattgct 5820
caaagagaca tgggtggaag agatgaaggt tacgattggt tgattatgac acccggtgtg 5880
ggtttagatg acaagggaga cgcattgggt caacagtata gaaccgtgga tgatgtggtc 5940
tctacaggat ctgacattat tattgttgga agaggactat ttgcaaaggg aagggatgct 6000
aaggtagagg gtgaacgtta cagaaaagca ggctgggaag catatttgag aagatgcggc 6060
cagcaaaact aaaaaactgt attataagta aatgcatgta tactaaactc acaaattaga 6120
gcttcaattt aattatatca gttattaccc gggaatctcg gtcgtaatga aaggaaaagc 6180
gcaacggacg ggcgagtaga ttgcgcaaca tgcgagcatg atccagagat ttctgaagca 6240
gcaaaaggat gttccatgta catgacgcgc ggcttgcggt aaattgttgg caaattttcc 6300
ggcgtagccc aaaacgcgct gtcgtcaagt cgttaagggc gtgcccttca tcatccgatc 6360
tggagtcaaa atgtcctcac gtaaagagct tgccaatgct attcgtgcgc tgagcatgga 6420
cgcagtacag aaagccaaat ccggtcaccc gggtgcccct atgggtatgg ctgacattgc 6480
cgaagtcctg tggcgtgatt tcctgaaaca caacccgcag aatccgtcct gggctgaccg 6540
tgaccgcttc gtgctgtcca acggccacgg ctccatgctg atctacagcc tgctgcacct 6600
caccggttac gatctgccga tggaagaact gaaaaacttc cgtcagctgc actctaaaac 6660
tccgggccac ccggaagtag gttataccgc tggtgtggaa accaccaccg gtccgctggg 6720
tcagggtatt gccaacgcag tcggtatggc gattgcagaa aaaacgctgg cggcgcagtt 6780
taaccgtcca ggtcacgaca ttgtcgacca ctacacctac gccttcatgg gcgacggctg 6840
catgatggaa ggcatctccc acgaagtttg ctctctggcg ggtacgctga agctgggtaa 6900
actgattgcg ttctacgatg acaacggtat ctcaatcgat ggtcacgttg aaggctggtt 6960
cactgacgac accgcaatgc gtttcgaagc ttacggctgg cacgttattc gcgacatcga 7020
cggtcatgac gcggcatcca tcaaacgcgc agtagaagaa gcgcgcgcag tgactgacaa 7080
accgtccctg ctgatgtgca aaaccatcat cggtttcggt tccccgaaca aagccggtac 7140
ccacgactcc cacggtgcgc cgctgggcga cgctgaaatt gccctgaccc gcgaacagct 7200
gggctggaaa tacgcgccgt tcgaaatccc gtctgaaatc tatgctcagt gggatgcgaa 7260
agaagcaggc caggcgaaag aatctgcatg gaatgagaag tttgcggctt acgcgaaagc 7320
ttatccgcag gaagcggctg aatttacccg ccgtatgaaa ggcgaaatgc cgtctgactt 7380
cgacgccaaa gcgaaagagt ttatcgctaa actgcaggct aatccggcga aaatcgccag 7440
ccgtaaagcg tcgcagaatg ctatcgaagc gttcggcccg ctgttgcctg aattcctcgg 7500
cggctctgct gacctggcac cgtctaacct gaccctgtgg tctggttcta aagcaatcaa 7560
cgaagatgct gcaggtaact acatccacta cggtgttcgc gagttcggta tgaccgcgat 7620
tgctaacggt atctccctgc acggtggttt cctgccgtac acctccacct tcctgatgtt 7680
cgtggaatac gcacgtaacg ccgtacgtat ggctgcgctg atgaaacagc gtcaggtgat 7740
ggtttacacc cacgactcca tcggtctggg cgaagatggc ccgactcacc agccggttga 7800
gcaggtcgct tctctgcgcg tgaccccgaa catgtctaca tggcgtccgt gtgaccaggt 7860
tgaatccgcg gtcgcgtgga aatacggcgt tgagcgtcag gacggcccga ctgcgcttat 7920
cctctcccgt cagaacctgg cgcagcagga acgaactgaa gagcaactgg caaacatcgc 7980
gcgcggtggt tatgtgctga aagactgcgc cggtcagccg gaactgattt tcatcgctac 8040
cggttcagaa gttgaactgg ctgttgctgc ctacgaaaaa ctgactgccg aaggcgtgaa 8100
agcgcgcgtg gtgtccatgc cgtctaccga cgcatttgac aagcaggatg ctgcttaccg 8160
tgaatccgta ctgccgaaag cggttactgc acgcgttgct gtagaagcgg gtattgctga 8220
ctactggtac aagtatgttg gcctgaacgg tgctatcgtc ggtatgacca ccttcggtga 8280
atctgctccg gcagagctgc tgtttgaaga gttcggcttc actgttgata acgttgttgc 8340
gaaagcaaaa gaactgctgt aattagcatt tcgggtaaaa aggtcgcttc ggcgaccttt 8400
tttattacct tgatatgtcc gtttgcggac aagcaataga taaagcgtgt tgtagatcac 8460
aaatatttat atgcaataaa tatcaattat gtaatatgca tcacgatatg cgtattgaca 8520
tttgttgtta taactataac tcaatgttat ataagaaatt aacttgaaat aattaacaaa 8580
caaaggagtt acagttagaa attgtaggag agatctcgtt tttcgcgaca atctggcgtt 8640
tttcttgcta attccaggat taatccgttc atagtgtaaa accccgttta cacattctga 8700
cggaagatat agattggaag tattgcattc actaagataa gtatggcaac actggaacag 8760
acatgaatta tcagaacgac gatttacgca tcaaagaaat caaagagtta cttcctcctg 8820
tcgcattgct ggaaaaattc cccgctactg aaaatgccgc gaatacggtt gcccatgccc 8880
gaaaagcgat ccataagatc ctgaaaggta atgatgatcg cctgttggtt gtgattggcc 8940
catgctcaat tcatgatcct gtcgcggcaa aagagtatgc cactcgcttg ctggcgctgc 9000
gtgaagagct gaaagatgag ctggaaatcg taatgcgcgt ctattttgaa aagccgcgta 9060
ccacggtggg ctggaaaggg ctgattaacg atccgcatat ggataatagc ttccagatca 9120
acgacggtct gcgtatagcc cgtaaattgc tgcttgatat taacgacagc ggtctgccag 9180
cggcaggtga gtttctcgat atgatcaccc cacaatatct cgctgacctg atgagctggg 9240
gcgcaattgg cgcacgtacc accgaatcgc aggtgcaccg cgaactggca tcagggcttt 9300
cttgtccggt cggcttcaaa aatggcaccg acggtacgat taaagtggct atcgatgcca 9360
ttaatgccgc cggtgcgccg cactgcttcc tgtccgtaac gaaatggggg cattcggcga 9420
ttgtgaatac cagcggtaac ggcgattgcc atatcattct gcgcggcggt aaagagccta 9480
actacagcgc gaagcacgtt gctgaagtga aagaagggct gaacaaagca ggcctgccag 9540
cacaggtgat gatcgatttc agccatgcta actcgtccaa acaattcaaa aagcagatgg 9600
atgtttgtgc tgacgtttgc cagcagattg ccggtggcga aaaggccatt attggcgtga 9660
tggtggaaag ccatctggtg gaaggcaatc agagcctcga gagcggggag ccgctggcct 9720
acggtaagag catcaccgat gcctgcatcg gctgggaaga taccgatgct ctgttacgtc 9780
aactggcgaa tgcagtaaaa gcgcgtcgcg ggtaaggttt aattgtcgga tgcgccgtca 9840
gagtggcgta tccgatgaat caccacaggc ctgataagtc gcgcagcgtc gcatcaggca 9900
atgtgctcca ttgttagcaa caaaaaagcc gactcacttg cagtcggctt tctcatttta 9960
aacgaatgac gtttacttcg ctttaccctg gtttgcaacc gccgctgctt tcgctctcga 10020
ggctattgac gacagctatg gttcactgtc caccaaccaa aactgtgctc agtaccgcca 10080
atatttctcc cttgaggggt acaaagaggt gtccctagaa gagatccacg ctgtgtaaaa 10140
attttacaaa aaggtattga ctttccctac agggtgtgta ataatttaat tacaggcggg 10200
ggcaaccccg cctgttctag aggaggagga atcgccatgg agaggattgt cgttactctc 10260
ggggaacgta gttacccaat taccatcgca tctggtttgt ttaatgaacc agcttcattc 10320
ttaccgctga aatcgggcga gcaggtcatg ttggtcacca acgaaaccct ggctcctctg 10380
tatctcgata aggtccgcgg cgtacttgaa caggcgggtg ttaacgtcga tagcgttatc 10440
ctccctgacg gcgagcagta taaaagcctg gctgtactcg ataccgtctt tacggcgttg 10500
ttacaaaagc cgcatggtcg cgatactacg ctggtggcgc ttggcggcgg cgtagtgggc 10560
gatctgaccg gcttcgcggc ggcgagttat cagcgcggtg ttcgtttcat tcaagtcccg 10620
acgacgttac tgtcgcaggt cgattcctcc gttggcggca aaactgcggt caaccatccc 10680
ctcggtaaaa acatgattgg cgcgttctac cagcctgctt cagtggtggt ggatctcgac 10740
tgtctgaaaa cgcttccccc gcgtgagtta gcgtcggggc tggcagaagt catcaaatac 10800
ggcattattc ttgacggtgc gtttttcaac tggctggaag agaatctgga tgcgttgttg 10860
cgtctggacg gtccggcaat ggcgtactgt attcgccgtt gttgtgaact gaaggcagaa 10920
gttgtcgccg ccgacgagcg cgaaaccggg ttacgtgctt tactgaatct gggacacacc 10980
tttggtcatg ccattgaagc tgaaatgggg tatggcaatt ggttacatgg tgaagcggtc 11040
gctgcgggta tggtgatggc ggcgcggacg tcggaacgtc tcgggcagtt tagttctgcc 11100
gaaacgcagc gtattataac cctgctcacg cgggctgggt taccggtcaa tgggccgcgc 11160
gaaatgtccg cgcaggcgta tttaccgcat atgctgcgtg acaagaaagt ccttgcggga 11220
gagatgcgct taattcttcc gttggcaatt ggtaagagtg aagttcgcag cggcgtttcg 11280
cacgagcttg ttcttaacgc cattgccgat tgtcaatcag cgtaatcatc gttcatgcct 11340
gatgccgcta tgtaggccgg ataaggcgtt cacgccgcat ccggcaaccg atgcctgatg 11400
cgacgcggtc gcgtcttatc aggcctacag gtcgatgccg atatgtacat cgtattcggc 11460
aattaataca tagca 11475
<210> 33
<211> 7769
<212> DNA
<213> 质粒pYEP24;
<400> 33
gaattctgaa ccagtcctaa aacgagtaaa taggaccggc aattcttcaa gcaataaaca 60
ggaataccaa ttattaaaag ataacttagt cagatcgtac aataaagctt tgaagaaaaa 120
tgcgccttat tcaatctttg ctataaaaaa tggcccaaaa tctcacattg gaagacattt 180
gatgacctca tttctttcaa tgaagggcct aacggagttg actaatgttg tgggaaattg 240
gagcgataag cgtgcttctg ccgtggccag gacaacgtat actcatcaga taacagcaat 300
acctgatcac tacttcgcac tagtttctcg gtactatgca tatgatccaa tatcaaagga 360
aatgatagca ttgaaggatg agactaatcc aattgaggag tggcagcata tagaacagct 420
aaagggtagt gctgaaggaa gcatacgata ccccgcatgg aatgggataa tatcacagga 480
ggtactagac tacctttcat cctacataaa tagacgcata taagtacgca tttaagcata 540
aacacgcact atgccgttct tctcatgtat atatatatac aggcaacacg cagatatagg 600
tgcgacgtga acagtgagct gtatgtgcgc agctcgcgtt gcattttcgg aagcgctcgt 660
tttcggaaac gctttgaagt tcctattccg aagttcctat tctctagaaa gtataggaac 720
ttcagagcgc ttttgaaaac caaaagcgct ctgaagacgc actttcaaaa aaccaaaaac 780
gcaccggact gtaacgagct actaaaatat tgcgaatacc gcttccacaa acattgctca 840
aaagtatctc tttgctatat atctctgtgc tatatcccta tataacctac ccatccacct 900
ttcgctcctt gaacttgcat ctaaactcga cctctacatt ttttatgttt atctctagta 960
ttactcttta gacaaaaaaa ttgtagtaag aactattcat agagtgaatc gaaaacaata 1020
cgaaaatgta aacatttcct atacgtagta tatagagaca aaatagaaga aaccgttcat 1080
aattttctga ccaatgaaga atcatcaacg ctatcacttt ctgttcacaa agtatgcgca 1140
atccacatcg gtatagaata taatcgggga tgcctttatc ttgaaaaaat gcacccgcag 1200
cttcgctagt aatcagtaaa cgcgggaagt ggagtcaggc tttttttatg gaagagaaaa 1260
tagacaccaa agtagccttc ttctaacctt aacggaccta cagtgcaaaa agttatcaag 1320
agactgcatt atagagcgca caaaggagaa aaaaagtaat ctaagatgct ttgttagaaa 1380
aatagcgctc tcgggatgca tttttgtaga acaaaaaaga agtatagatt ctttgttggt 1440
aaaatagcgc tctcgcgttg catttctgtt ctgtaaaaat gcagctcaga ttctttgttt 1500
gaaaaattag cgctctcgcg ttgcattttt gttttacaaa aatgaagcac agattcttcg 1560
ttggtaaaat agcgctttcg cgttgcattt ctgttctgta aaaatgcagc tcagattctt 1620
tgtttgaaaa attagcgctc tcgcgttgca tttttgttct acaaaatgaa gcacagatgc 1680
ttcgttaaca aagatatgct attgaagtgc aagatggaaa cgcagaaaat gaaccgggga 1740
tgcgacgtgc aagattacct atgcaataga tgcaatagtt tctccaggaa ccgaaataca 1800
tacattgtct tccgtaaagc gctagactat atattattat acaggttcaa atatactatc 1860
tgtttcaggg aaaactccca ggttcggatg ttcaaaattc aatgatgggt aacaagtacg 1920
atcgtaaatc tgtaaaacag tttgtcggat attaggctgt atctcctcaa agcgtattcg 1980
aatatcattg agaagctgca gcgtcacatc ggataataat gatggcagcc attgtagaag 2040
tgccttttgc atttctagtc tctttctcgg tctagctagt tttactacat cgcgaagata 2100
gaatcttaga tcacactgcc tttgctgagc tggatcaata gagtaacaaa agagtggtaa 2160
ggcctcgtta aaggacaagg acctgagcgg aagtgtatcg tacagtagac ggagtatact 2220
agtatagtct atagtccgtg gaattctcat gtttgacagc ttatcatcga taagcttttc 2280
aattcaattc atcatttttt ttttattctt ttttttgatt tcggtttctt tgaaattttt 2340
ttgattcggt aatctccgaa cagaaggaag aacgaaggaa ggagcacaga cttagattgg 2400
tatatatacg catatgtagt gttgaagaaa catgaaattg cccagtattc ttaacccaac 2460
tgcacagaac aaaaacctgc aggaaacgaa gataaatcat gtcgaaagct acatataagg 2520
aacgtgctgc tactcatcct agtcctgttg ctgccaagct atttaatatc atgcacgaaa 2580
agcaaacaaa cttgtgtgct tcattggatg ttcgtaccac caaggaatta ctggagttag 2640
ttgaagcatt aggtcccaaa atttgtttac taaaaacaca tgtggatatc ttgactgatt 2700
tttccatgga gggcacagtt aagccgctaa aggcattatc cgccaagtac aattttttac 2760
tcttcgaaga cagaaaattt gctgacattg gtaatacagt caaattgcag tactctgcgg 2820
gtgtatacag aatagcagaa tgggcagaca ttacgaatgc acacggtgtg gtgggcccag 2880
gtattgttag cggtttgaag caggcggcag aagaagtaac aaaggaacct agaggccttt 2940
tgatgttagc agaattgtca tgcaagggct ccctatctac tggagaatat actaagggta 3000
ctgttgacat tgcgaagagc gacaaagatt ttgttatcgg ctttattgct caaagagaca 3060
tgggtggaag agatgaaggt tacgattggt tgattatgac acccggtgtg ggtttagatg 3120
acaagggaga cgcattgggt caacagtata gaaccgtgga tgatgtggtc tctacaggat 3180
ctgacattat tattgttgga agaggactat ttgcaaaggg aagggatgct aaggtagagg 3240
gtgaacgtta cagaaaagca ggctgggaag catatttgag aagatgcggc cagcaaaact 3300
aaaaaactgt attataagta aatgcatgta tactaaactc acaaattaga gcttcaattt 3360
aattatatca gttattaccc gggaatctcg gtcgtaatga tttttataat gacgaaaaaa 3420
aaaaaattgg aaagaaaaag ctttaatgcg gtagtttatc acagttaaat tgctaacgca 3480
gtcaggcacc gtgtatgaaa tctaacaatg cgctcatcgt catcctcggc accgtcaccc 3540
tggatgctgt aggcataggc ttggttatgc cggtactgcc gggcctcttg cgggatatcg 3600
tccattccga cagcatcgcc agtcactatg gcgtgctgct agcgctatat gcgttgatgc 3660
aatttctatg cgcacccgtt ctcggagcac tgtccgaccg ctttggccgc cgcccagtcc 3720
tgctcgcttc gctacttgga gccactatcg actacgcgat catggcgacc acacccgtcc 3780
tgtggatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg 3840
ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca 3900
tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg 3960
ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac 4020
tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaccgatg cccttgagag 4080
ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta 4140
tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt 4200
tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg 4260
gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg 4320
agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt 4380
tcgcgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg 4440
ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc 4500
ttcaaggatc gctcgcggct cttaccagcc taacttcgat cactggaccg ctgatcgtca 4560
cggcgattta tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg 4620
ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga 4680
cctgaatgga agccggcggc acctcgctaa cggattcacc actccaagaa ttggagccaa 4740
tcaattcttg cggagaactg tgaatgcgca aaccaaccct tggcagaaca tatccatcgc 4800
gtccgccatc tccagcagcc gcacgcggcg catctcgggc agcgttgggt cctggccacg 4860
ggtgcgcatg atcgtgctcc tgtcgttgag gacccggcta ggctggcggg gttgccttac 4920
tggttagcag aatgaatcac cgatacgcga gcgaacgtga agcgactgct gctgcaaaac 4980
gtctgcgacc tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt aaagtctgga 5040
aacgcggaag tcagcgccct gcaccattat gttccggatc tgcatcgcag gatgctgctg 5100
gctaccctgt ggaacaccta catctgtatt aacgaagcgc tggcattgac cctgagtgat 5160
ttttctctgg tcccgccgca tccataccgc cagttgttta ccctcacaac gttccagtaa 5220
ccgggcatgt tcatcatcag taacccgtat cgtgagcatc ctctctcgtt tcatcggtat 5280
cattaccccc atgaacagaa attccccctt acacggaggc atcaagtgac caaacaggaa 5340
aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct tctggagaaa 5400
ctcaacgagc tggacgcgga tgaacaggca gacatctgtg aatcgcttca cgaccacgct 5460
gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac 5520
atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc 5580
cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt 5640
agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag 5700
tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc 5760
gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 5820
tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 5880
agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 5940
cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 6000
ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 6060
tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 6120
gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 6180
gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 6240
gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 6300
ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 6360
ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag 6420
ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 6480
gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 6540
ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 6600
tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 6660
ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 6720
gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 6780
tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 6840
cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 6900
ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 6960
gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg 7020
caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 7080
gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 7140
ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 7200
tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 7260
caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 7320
cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 7380
cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 7440
ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 7500
aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 7560
tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 7620
gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 7680
gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata 7740
ggcgtatcac gaggcccttt cgtcttcaa 7769
<210> 34
<211> 2000
<212> DNA
<213> 缺失的aroE区的序列;
<400> 34
actacgtccg tcctctgaaa tcttcagcgg atggacatat cgtcaaagtt ctggaggggc 60
aggtttgccc tgcatgtggc gcaaatctgg tattacgcca gggacgcttt ggtatgttta 120
ttggttgcat taactaccct gaatgcgaac ataccgaact tatcgataaa ccggacgaaa 180
cagcaattac atgcccccaa tgtcggacgg gccatctggt ccagcgccgc tcccgttatg 240
gcaaaacatt tcactcttgt gatcgctacc cggagtgtca atttgccatt aacttcaaac 300
ccatagctgg agaatgccct gagtgtcatt atccgctact catcgaaaag aaaaccgcgc 360
agggtgtaaa acacttttgt gccagtaaac aatgtggaaa gccggtttcg gcggaataat 420
aacgtgaata ataacctgca aagagacgct atcgcagctg cgatagatgt tctcaatgaa 480
gaacgtgtca tcgcctatcc aacggaagcc gttttcggtg ttgggtgcga tcctgatagc 540
gaaacagcag tgatgcgact gttggagtta aaacagcgtc cggttgataa ggggctgatt 600
ttaatcgcag caaattacga gcagcttaaa ccctatattg atgacaccat gttgactgac 660
gtgcagcgtg aaaccatttt ttcccgctgg ccaggtcctg tcacctttgt ctttcccgcg 720
cctgcgacaa caccgcgctg gttgacgggc cgctttgatt cgcttgctgt acgagtcacc 780
gaccatccgt tggtggttgc tttgtgccag gcttatggta aaccgctggt ttctaccagt 840
gccaacttga gtggattgcc accttgtcga acagtagacg aagttcgcgc acaatttggc 900
gcggcgttcc cggttgtgcc tggtgaaacg ggggggcgtt taaatccttc agaaatccgc 960
gatgccctga cgggtgaact gtttcgacag gggtaacata atcaggccat ccagtttccg 1020
gacagggaag agtgggacga gaataaaaaa tgtgtatgtt ttcccgctct cgtgaatggt 1080
atgcaactga catgcgcgat ctctggcgag agtctggcgt atcgctttac tggagatacg 1140
ccagaacagt ggttagcgag ttttcgtcag catcgctggg acctggaaga agaagcggaa 1200
aacttaattc aggaacaaag tgaagatgat caaggctggg tctggttacc ctgatccaga 1260
tattcgtcct tccatttcac gtaattattc gcggaatagc gtaacccagc cttctcttca 1320
tcacttaacg ggcggatctg tttgacgggg ctaccgagat acagatatcc gctctccagc 1380
cgtttatttt gtgggaccag actacccgca ccaatcatca catcatcttc tactattgcg 1440
ccatcaagta aaattgagcc catcccaacc aaaactcgat tgccaatggt gcagccgtgg 1500
agcatcacct tgtgaccaac agtgacatct tcgccaatgg ttaatgggtt gccatctggg 1560
ttgtacgagg atttatgagt gacatgcaac atactgccat cctggatatt ggtgcgtgct 1620
ccgatctgta cataatgtac atctccacga atcacaacga gcggccagat ccccacatca 1680
tcagccagac gaacgtcacc aatcacgaca ctgctatcgt cgatcattac gcgctgaccg 1740
atttgtggaa aaagatcgcg gtatgggcgt aaaacatcag acatacttac ctcagcaata 1800
aatgatttac taatgacttt gggggcatta ttggccttgt gcaagtcttt tagtatgcaa 1860
aaaagcaccg ttttgtgtgc gattgcagca aaaagggtga aaaaacaaca aacagaaaaa 1920
aagatcaaaa aaatacttgt gcaaaaaatt gggatcccta taatgcgcct ccgttgagac 1980
gacaacgtga aacacttcac 2000
<210> 35
<211> 2460
<212> DNA
<213> 在缺失的ack基因座处整合的P15aroB盒的序列;
<400> 35
gatcggcggc ataaaacgga tcgcataacg cgtcatcttg ataacgcgat tttcgacaaa 60
gaccggggca aggcgttttt ccagcggcca cgtctttgag taatgctgtc cccggcgaaa 120
caagctaaaa aaattaacag aacgattatc cggcgttgac atgcttcacc tcaacttcac 180
atataaagat tcaaaaattt gtgcaaattc acaactcagc gggacaacgt tcaaaacatt 240
ttgtcttcca tacccactat caggtatcct ttagcagcct gaaggcctaa gtagtacata 300
ttcattgagt cgtcaaattc atatacatta tgccattggc tgaaaattac gcaaaatggc 360
atagactcaa gatatttctt ccatcatgca aaaaaaaatt tgcagtgcat gatgttaatc 420
ataaatgtcg gtgtcatcat gcgctacgct ctatggctcc ctgacgtttt tttagccacg 480
tatcaattat aggtacttcc ctcgaggcta ttgacgacag ctatggttca ctgtccacca 540
accaaaactg tgctcagtac cgccaatatt tctcccttga ggggtacaaa gaggtgtccc 600
tagaagagat ccacgctgtg taaaaatttt acaaaaaggt attgactttc cctacagggt 660
gtgtaataat ttaattacag gcgggggcaa ccccgcctgt tctagaggag gaggaatcgc 720
catggagagg attgtcgtta ctctcgggga acgtagttac ccaattacca tcgcatctgg 780
tttgtttaat gaaccagctt cattcttacc gctgaaatcg ggcgagcagg tcatgttggt 840
caccaacgaa accctggctc ctctgtatct cgataaggtc cgcggcgtac ttgaacaggc 900
gggtgttaac gtcgatagcg ttatcctccc tgacggcgag cagtataaaa gcctggctgt 960
actcgatacc gtctttacgg cgttgttaca aaagccgcat ggtcgcgata ctacgctggt 1020
ggcgcttggc ggcggcgtag tgggcgatct gaccggcttc gcggcggcga gttatcagcg 1080
cggtgttcgt ttcattcaag tcccgacgac gttactgtcg caggtcgatt cctccgttgg 1140
cggcaaaact gcggtcaacc atcccctcgg taaaaacatg attggcgcgt tctaccagcc 1200
tgcttcagtg gtggtggatc tcgactgtct gaaaacgctt cccccgcgtg agttagcgtc 1260
ggggctggca gaagtcatca aatacggcat tattcttgac ggtgcgtttt tcaactggct 1320
ggaagagaat ctggatgcgt tgttgcgtct ggacggtccg gcaatggcgt actgtattcg 1380
ccgttgttgt gaactgaagg cagaagttgt cgccgccgac gagcgcgaaa ccgggttacg 1440
tgctttactg aatctgggac acacctttgg tcatgccatt gaagctgaaa tggggtatgg 1500
caattggtta catggtgaag cggtcgctgc gggtatggtg atggcggcgc ggacgtcgga 1560
acgtctcggg cagtttagtt ctgccgaaac gcagcgtatt ataaccctgc tcacgcgggc 1620
tgggttaccg gtcaatgggc cgcgcgaaat gtccgcgcag gcgtatttac cgcatatgct 1680
gcgtgacaag aaagtccttg cgggagagat gcgcttaatt cttccgttgg caattggtaa 1740
gagtgaagtt cgcagcggcg tttcgcacga gcttgttctt aacgccattg ccgattgtca 1800
atcagcgtaa tcatcgttca tgcctgatgc cgctatgtag gccggataag gcgttcacgc 1860
cgcatccggc aaccgatgcc tgatgcgacg cggtcgcgtc ttatcaggcc tacaggtcga 1920
tgccgatatg tacatcgtat tcggcaatta atacatagca tttcacaccg ccagctcagc 1980
tggcggtgct gttttgtaac ccgccaaatc ggcggtaacg aaagaggata aaccgtgtcc 2040
cgtattatta tgctgatccc taccggaacc agcgtcggtc tgaccagcgt cagccttggc 2100
gtgatccgtg caatggaacg caaaggcgtt cgtctgagcg ttttcaaacc tatcgctcag 2160
ccgcgtaccg gtggcgatgc gcccgatcag actacgacta tcgtgcgtgc gaactcttcc 2220
accacgacgg ccgctgaacc gctgaaaatg agctacgttg aaggtctgct ttccagcaat 2280
cagaaagatg tgctgatgga agagatcgtc gcaaactacc acgctaacac caaagacgct 2340
gaagtcgttc tggttgaagg tctggtcccg acacgtaagc accagtttgc ccagtctctg 2400
aactacgaaa tcgctaaaac gctgaatgcg gaaatcgtct tcgttatgtc tcagggcact 2460
<210> 36
<211> 1000
<212> DNA
<213> 缺失的poxB区的序列;
<400> 36
gcggcccggc tccgtatatg gattgggtag agcaggaagt gaaagcgctc ggcgtgacgc 60
gtttctttaa agagaaattc ttcaccccag tagcggaagc agcgaccagc ggtctgaaat 120
tcaccaaact gcaaccggca cgagaatttt acgccccggt tggcaccacg ctactggagg 180
cgctggaaag caataacgtt ccggttgtcg ccgcctgccg tgcgggtgtt tgcggctgct 240
gtaagacaaa agtggtttcc ggtgaatata cggtgagcag cacaatgacg ctgaccgacg 300
ccgaaatcgc tgaaggttac gtactggcct gctcctgcca tccgcagggg gatttggttc 360
tcgcataatc gccttatgcc cgatgatatt cctttcatcg ggctatttaa ccgttagtgc 420
ctcctttctc tcccatccct tccccctccg tcagatgaac taaacttgtt accgttatca 480
cattcaggag atggagaacc aaagggtggc atttcccgtc ataataagga catgccatga 540
ttgatttacg cagtgatacc gttacccgac cgagccgcgc catgctcgaa gcaatgatgg 600
ccgccccggt tggggacgac gtttacggag acgaccctac cgttaatgct ctgcaggact 660
acgccgcaga gctttccggt aaagaagccg ccatttttct gccgaccggc actcaggcca 720
acctggtcgc tctgctcagt cactgcgaac gtggcgaaga gtatattgtc ggtcaggccg 780
cgcataacta tctgtttgaa gccggtggcg cagcggtgct gggcagtatt cagccgcaac 840
ccatcgacgc ggctgccgac ggcacgctac cgctggataa agtggcgatg aaaatcaaac 900
ccgacgatat ccatttcgcc cgcaccaaat tactcagtct ggaaaacacc cacaacggca 960
aagtgctgcc gcgtgaatac ctgaaagaag catgggaatt 1000
<210> 37
<211> 3392
<212> DNA
<213> 在缺失的poxB基因座处的tktA盒;
<400> 37
gcggcccggc tccgtatatg gattgggtag agcaggaagt gaaagcgctc ggcgtgacgc 60
gtttctttaa agagaaattc ttcaccccag tagcggaagc agcgaccagc ggtctgaaat 120
tcaccaaact gcaaccggca cgagaatttt acgccccggt tggcaccacg ctactggagg 180
cgctggaaag caataacgtt ccggttgtcg ccgcctgccg tgcgggtgtt tgcggctgct 240
gtaagacaaa agtggtttcc ggtgaatata cggtgagcag cacaatgacg ctgaccgacg 300
ccgaaatcgc tgaaggttac gtactggcct gctcctgcca tccgcagggg gatttggttc 360
tcgcataatc gccttatgcc cgatgatatt cctttcatcg ggctatttaa ccgttagtgc 420
ctcctttctc tcccatccct tccccctccg tcagatgaac taaacttgtt accgttatca 480
cattcaggag atggagaacc aaggaaaagc gcaacggacg ggcgagtaga ttgcgcaaca 540
tgcgagcatg atccagagat ttctgaagca gcaaaaggat gttccatgta catgacgcgc 600
ggcttgcggt aaattgttgg caaattttcc ggcgtagccc aaaacgcgct gtcgtcaagt 660
cgttaagggc gtgcccttca tcatccgatc tggagtcaaa atgtcctcac gtaaagagct 720
tgccaatgct attcgtgcgc tgagcatgga cgcagtacag aaagccaaat ccggtcaccc 780
gggtgcccct atgggtatgg ctgacattgc cgaagtcctg tggcgtgatt tcctgaaaca 840
caacccgcag aatccgtcct gggctgaccg tgaccgcttc gtgctgtcca acggccacgg 900
ctccatgctg atctacagcc tgctgcacct caccggttac gatctgccga tggaagaact 960
gaaaaacttc cgtcagctgc actctaaaac tccgggccac ccggaagtag gttataccgc 1020
tggtgtggaa accaccaccg gtccgctggg tcagggtatt gccaacgcag tcggtatggc 1080
gattgcagaa aaaacgctgg cggcgcagtt taaccgtcca ggtcacgaca ttgtcgacca 1140
ctacacctac gccttcatgg gcgacggctg catgatggaa ggcatctccc acgaagtttg 1200
ctctctggcg ggtacgctga agctgggtaa actgattgcg ttctacgatg acaacggtat 1260
ctcaatcgat ggtcacgttg aaggctggtt cactgacgac accgcaatgc gtttcgaagc 1320
ttacggctgg cacgttattc gcgacatcga cggtcatgac gcggcatcca tcaaacgcgc 1380
agtagaagaa gcgcgcgcag tgactgacaa accgtccctg ctgatgtgca aaaccatcat 1440
cggtttcggt tccccgaaca aagccggtac ccacgactcc cacggtgcgc cgctgggcga 1500
cgctgaaatt gccctgaccc gcgaacagct gggctggaaa tacgcgccgt tcgaaatccc 1560
gtctgaaatc tatgctcagt gggatgcgaa agaagcaggc caggcgaaag aatctgcatg 1620
gaatgagaag tttgcggctt acgcgaaagc ttatccgcag gaagcggctg aatttacccg 1680
ccgtatgaaa ggcgaaatgc cgtctgactt cgacgccaaa gcgaaagagt ttatcgctaa 1740
actgcaggct aatccggcga aaatcgccag ccgtaaagcg tcgcagaatg ctatcgaagc 1800
gttcggcccg ctgttgcctg aattcctcgg cggctctgct gacctggcac cgtctaacct 1860
gaccctgtgg tctggttcta aagcaatcaa cgaagatgct gcaggtaact acatccacta 1920
cggtgttcgc gagttcggta tgaccgcgat tgctaacggt atctccctgc acggtggttt 1980
cctgccgtac acctccacct tcctgatgtt cgtggaatac gcacgtaacg ccgtacgtat 2040
ggctgcgctg atgaaacagc gtcaggtgat ggtttacacc cacgactcca tcggtctggg 2100
cgaagatggc ccgactcacc agccggttga gcaggtcgct tctctgcgcg tgaccccgaa 2160
catgtctaca tggcgtccgt gtgaccaggt tgaatccgcg gtcgcgtgga aatacggcgt 2220
tgagcgtcag gacggcccga ctgcgcttat cctctcccgt cagaacctgg cgcagcagga 2280
acgaactgaa gagcaactgg caaacatcgc gcgcggtggt tatgtgctga aagactgcgc 2340
cggtcagccg gaactgattt tcatcgctac cggttcagaa gttgaactgg ctgttgctgc 2400
ctacgaaaaa ctgactgccg aaggcgtgaa agcgcgcgtg gtgtccatgc cgtctaccga 2460
cgcatttgac aagcaggatg ctgcttaccg tgaatccgta ctgccgaaag cggttactgc 2520
acgcgttgct gtagaagcgg gtattgctga ctactggtac aagtatgttg gcctgaacgg 2580
tgctatcgtc ggtatgacca ccttcggtga atctgctccg gcagagctgc tgtttgaaga 2640
gttcggcttc actgttgata acgttgttgc gaaagcaaaa gaactgctgt aattagcatt 2700
tcgggtaaaa aggtcgcttc ggcgaccttt tttattacct tgatatgtcc gtttgcggac 2760
aagcaataga taaagcgtgt tgtagatcac aaatatttat atgcaataaa tatcaattat 2820
gtaatatgca tcacgatatg cgtattgaca tttgttgtta taactataac tcaatgttat 2880
ataagaaatt aaaaagggtg gcatttcccg tcataataag gacatgccat gattgattta 2940
cgcagtgata ccgttacccg accgagccgc gccatgctcg aagcaatgat ggccgccccg 3000
gttggggacg acgtttacgg agacgaccct accgttaatg ctctgcagga ctacgccgca 3060
gagctttccg gtaaagaagc cgccattttt ctgccgaccg gcactcaggc caacctggtc 3120
gctctgctca gtcactgcga acgtggcgaa gagtatattg tcggtcaggc cgcgcataac 3180
tatctgtttg aagccggtgg cgcagcggtg ctgggcagta ttcagccgca acccatcgac 3240
gcggctgccg acggcacgct accgctggat aaagtggcga tgaaaatcaa acccgacgat 3300
atccatttcg cccgcaccaa attactcagt ctggaaaaca cccacaacgg caaagtgctg 3360
ccgcgtgaat acctgaaaga agcatgggaa tt 3392
<210> 38
<211> 1045
<212> DNA
<213> ptsHI缺失序列;
<400> 38
gaagatgaaa gctttaccaa caagaatatt gtggttattc taccatcatc gggtgagcgt 60
tatttaagca ccgcattgtt tgccgatctc ttcactgaga aagaattgca acagtaatgc 120
cagcttgtta aaaatgcgta aaaaagcacc tttttaggtg cttttttgtg gcctgcttca 180
aactttcgcc cctcctggca ttgattcagc ctgtcggaac tggtatttaa ccagactaat 240
tattttgatg cgcgaaatta atcgttacag gaaaagccaa agctgaatcg attttatgat 300
ttggttcaat tcttccttta gcggcataat gtttaatgac gtacgaaacg tcagcggtca 360
acacccgcca gcaatggact gtattgcgct cttcgtgcgt cgcgtctgtt aaaaactggc 420
gctaacaata caggctaaag tcgaaccgcc aggctagact ttagttccac aacactaaac 480
ctataagttg gggaaataca atgttccagc aagaagttac cattaccgct ccgacaatct 540
gctaatccac gagatgcggc ccaatttact gcttaggaga agatcatggg tttgttcgat 600
aaactgaaat ctctggtttc cgacgacaag aaggataccg gaactattga gatcattgct 660
ccgctctctg gcgagatcgt caatatcgaa gacgtgccgg atgtcgtttt tgcggaaaaa 720
atcgttggtg atggtattgc tatcaaacca acgggtaaca aaatggtcgc gccagtagac 780
ggcaccattg gtaaaatctt tgaaaccaac cacgcattct ctatcgaatc tgatagcggc 840
gttgaactgt tcgtccactt cggtatcgac accgttgaac tgaaaggcga aggcttcaag 900
cgtattgctg aagaaggtca gcgcgtgaaa gttggcgata ctgtcattga atttgatctg 960
ccgctgctgg aagagaaagc caagtctacc ctgactccgg ttgttatctc caacatggac 1020
gaaatcaaag aactgatcaa actgt 1045
<210> 39
<211> 4595
<212> DNA
<213> 在缺失的tdc基因座处整合的glf-glk盒;
<400> 39
ctgatttctt tgtcgctgat cccttactgg aactctgcag ttatcgacca ggttgacctc 60
ggttcgctgt cgttaaccgg tcatgacggt atcctgatca ctgtctggct ggggatttcc 120
atcatggttt tctcctttaa cttctcgcca atcgtctctt ccttcgtggt ttctaaacgt 180
gaagagtatg agaaagactt cggtcgcgac ttcaccgaac gtaaatgttc ccaaatcatt 240
tctcgtgcca gcatgctgat ggttgcagtg gtgatgttct ttgcctttag ctgcctgttt 300
actctgtctc cggccaacat ggcggaagcc aaagcgcaga atattccagt gctttcttat 360
ctggctaacc actttgcgtc catgaccggt accaaaacaa cgttcgcgat tacactggaa 420
tatgcggctt ccatcatcgc actcgtggct atcttcaaat ctttcttcgg tcactatctg 480
gggacgctgg aaggcttgaa tggtctgatt ctgaagttcg gttataaagg tgacaaaacc 540
aaagtgtcgc tgggtaaact gaatactctc agcatgatct tcatcatggg ctccacctgg 600
gttgttgcct acgccaaccc gaacatcctc gacctgattg aagccatggg cgcaccgatt 660
atcgcatccc tgctgtgcct gttgccgatg tatgccatcc gtaaagcgcc gtctctggcg 720
aaataccgtg gtcgtctgga taacgtgttt gttaccgtga ttggtctgct gaccatcctg 780
aacatcgtat acaaactgtt ttaatccgta actcaggatg agaaaagaga tgaatgaatt 840
tccggttgtt ttggttatta actgtggttc gtcttcgatt aagttttccg tactcgatgc 900
cagcgactgt gaagtattaa tgtcaggtat tgccgacggt attaactcgg aaaatgcatt 960
cttatccgta aatgggggag agccagcacc gctggctcac cacagctacg aaggtgcatt 1020
gaaggcaatt gcatttgaac tggaaaaacg gagtttaaat gacagcgtgg ccttaattgg 1080
tgcaaaagtg gctgtgactg taaaaagaaa tcgaaaaaga ccgttttgtg tgaaaacggt 1140
ctttttgttt ccttttaacc aactgccata actcgaggcc tacctagctt ccaagaaaga 1200
tatcctaaca gcacaagagc ggaaagatgt tttgttctac atccagaaca acctctgcta 1260
aaattcctga aaaattttgc aaaaagttgt tgactttatc tacaaggtgt ggtataataa 1320
tcttaacaac agcaggacgc tctagaggga gaggaatcgc catgagttct gaaagtagtc 1380
agggtctagt cacgcgacta gccctaatcg ctgctatagg cggcttgctt ttcggttacg 1440
attcagcggt tatcgctgca atcggtacac cggttgatat ccattttatt gcccctcgtc 1500
acctgtctgc tacggctgcg gcttcccttt ctgggatggt cgttgttgct gttttggtcg 1560
gttgtgttac cggttctttg ctgtctggct ggattggtat tcgcttcggt cgtcgcggcg 1620
gattgttgat gagttccatt tgtttcgtcg ccgccggttt tggtgctgcg ttaaccgaaa 1680
aattatttgg aaccggtggt tcggctttac aaattttttg ctttttccgg tttcttgccg 1740
gtttaggtat cggtgtcgtt tcaaccttga ccccaaccta tattgctgaa attgctccgc 1800
cagacaaacg tggtcagatg gtttctggtc agcagatggc cattgtgacg ggtgctttaa 1860
ccggttatat ctttacctgg ttactggctc atttcggttc tatcgattgg gttaatgcca 1920
gtggttggtg ctggtctccg gcttcagaag gcctgatcgg tattgccttc ttattgctgc 1980
tgttaaccgc accggatacg ccgcattggt tggtgatgaa gggacgtcat tccgaggcta 2040
gcaaaatcct tgctcgtctg gaaccgcaag ccgatcctaa tctgacgatt caaaagatta 2100
aagctggctt tgataaagcc atggacaaaa gcagcgcagg tttgtttgct tttggtatca 2160
ccgttgtttt tgccggtgta tccgttgctg ccttccagca gttagtcggt attaacgccg 2220
tgctgtatta tgcaccgcag atgttccaga atttaggttt tggagctgat acggcattat 2280
tgcagaccat ctctatcggt gttgtgaact tcatcttcac catgattgct tcccgtgttg 2340
ttgaccgctt cggccgtaaa cctctgctta tttggggtgc tctcggtatg gctgcaatga 2400
tggctgtttt aggctgctgt ttctggttca aagtcggtgg tgttttgcct ttggcttctg 2460
tgcttcttta tattgcagtc tttggtatgt catggggccc tgtctgctgg gttgttctgt 2520
cagaaatgtt cccgagttcc atcaagggcg cagctatgcc tatcgctgtt accggacaat 2580
ggttagctaa tatcttggtt aacttcctgt ttaaggttgc cgatggttct ccagcattga 2640
atcagacttt caaccacggt ttctcctatc tcgttttcgc agcattaagt atcttaggtg 2700
gcttgattgt tgctcgcttc gtgccggaaa ccaaaggtcg gagcctggat gaaatcgagg 2760
agatgtggcg ctcccagaag tagttaaact tgctttggct gaatcctttt gtctttttta 2820
gataagtctt aaccaattat actttttgtt tacaacgatg gtataaagcg ggcggactta 2880
ttttacctgt tgggtagcct tctgatttca gaaaggaatt attatggaaa ttgttgcgat 2940
tgacatcggt ggaacgcatg cgcgtttctc tattgcggaa gtaagcaatg gtcgggttct 3000
ttctcttgga gaagaaacaa cttttaaaac ggcagaacat gctagcttgc agttagcttg 3060
ggaacgtttc ggtgaaaaac tgggtcgtcc tctgccacgt gccgcagcta ttgcatgggc 3120
tggcccggtt catggtgaag ttttaaaact taccaataac ccttgggtat taagaccagc 3180
tactctgaat gaaaagctgg acatcgatac gcatgttctg atcaatgact tcggcgcggt 3240
tgcccacgcg gttgcgcata tggattcttc ttatctggat catatttgtg gtcctgatga 3300
agcgcttcct agcgatggtg ttatcactat tcttggtccg ggaacgggct tgggtgttgc 3360
ccatctgttg cggactgaag gccgttattt cgtcatcgaa actgaaggcg gtcatatcga 3420
ctttgctccg cttgacagac ttgaagacaa aattctggca cgtttacgtg aacgtttccg 3480
ccgcgtttct atcgaacgca ttatttctgg cccgggtctt ggtaatatct acgaagcact 3540
ggctgccatt gaaggcgttc cgttcagctt gctggatgat attaaattat ggcagatggc 3600
tttggaaggt aaagacaacc ttgctgaagc cgctttggat cgcttctgct tgagccttgg 3660
cgctatcgct ggtgatcttg ctttggcaca gggtcgaacc agtgttgtta ttggcggtgg 3720
tgtcggtctt cgtatcgctt cccatttgcc agaatctggt ttccgtcagc gctttgtttc 3780
aaaaggacgc tttgaacgcg tcatgtccaa gattccggtt aagttgatta cttatccgca 3840
gcctggactg ttgggtgcgc agctgcctat gccaacaaat attctgaagt tgaataatat 3900
tttttaatat tatgaactga atttaagagg ctgccttccg ataaaatcgg gaggtggcct 3960
tttttatatt ttttactaaa aaatgaagac aaaaaagtct taagtaagaa taatattatt 4020
attaactttt gatatatttt gtattagtgg atccgccctc ccgctggaaa ttgaagccat 4080
cgcagtacgt agtgcgtaaa gcctcgtgag cgggacggtc gtaaggtcgt tccgctccac 4140
ttcactgaac ggcaatccga gggtgtggat atgattagtg cattcgatat tttcaaaatt 4200
gggattggtc cctccagttc gcataccgtg gggccaatga atgccggaaa aagttttatt 4260
gatcggctgg aaagtagcgg cttattaacc gcgacgagcc atattgtggt cgatctgtac 4320
gggtcgttgt cactgacggg caaaggccat gccacggatg tcgccatcat catgggactg 4380
gcaggaaaca gtccgcagga tgttgtcatt gatgagatcc ctgcatttat agagttagta 4440
acgcgcagcg ggcggctgcc agtggcatct ggtgcgcata ttgttgattt tcctgtagca 4500
aagaacatta tcttccatcc cgaaatgttg cctcgccatg agaacggaat gcggatcact 4560
gcctggaagg gacaggaaga gctattaagt aaaac 4595
<210> 40
<211> 1069
<212> DNA
<213> galP缺失区;
<400> 40
actttggtcg tgaacatttc ccgtgggaaa aaaccgacaa agcgcagctg ctgcgcgatg 60
ctgccggtct gaagtaatct ttcttcacct gcgttcaaag gccagcctcg cgctggcctt 120
tttcttttgg ataggcgttc acgccgcatc cggcaaaaaa accgcccgca caataacatc 180
attcttcctg atcacgtttc accgcagatt atcatcacaa ctgaaaccga ttacaccaac 240
cacaacagac aaagatttgt aatattttca tattattatt cggttttcac agttgttaca 300
tttcttttca gtaaagtctt aattgcagat aacagcgttt aatctatgat gatataactc 360
aattattttc atgcacttaa atcataacta agataaatgt tagtgtaagc gattacactg 420
atgtgatttg cttcacatct ttttacgtcg tactcaccta tcttaattca caataaaaaa 480
taaccatatt ggagggcatc atgcctgacg ctaaaaaaca ggggcggtca aacaaggcaa 540
tgacgtttga aataggcgct cacgattaat ctccccaagc ttcctcccat cgcggaggaa 600
gccacctctt gcagtcatct tttcttcgct ctatcctctg ccgctatgaa aacatcccgt 660
ctccctatcg ccatccaaca ggccgttatg cgtcgcctgc gggaaaaact cgcccaggcc 720
aacctgaagc tagggcgtaa ctacccggag ccaaaactct cttacaccca gcgcggaacc 780
tccgccggaa cggcctggct ggaaagctat gaaattcgcc tcaatcccgt tttgctgttg 840
gaaaacagtg aagcttttat tgaagaagtg gtaccgcacg aactggcaca tttgctggta 900
tggaaacatt tcggccgcgt agcgccacat ggcaaagagt ggaagtggat gatggaaaac 960
gtgctgggtg ttcccgcccg tcgtacgcat cagttcgaac tgcaatccgt gcgtcgcaac 1020
accttcccct accgctgcaa gtgccaggag catcagctta ccgtacgcc 1069
<210> 41
<211> 6100
<212> DNA
<213> adhE基因座处的P15-catAX +PR-aroY+P26-quiC盒;
<400> 41
ttgattttca taggttaagc aaatcatcac cgcactgact atactctcgt attcgagcag 60
atgatttact aaaaaagttt aacattatca ggagagcatt agcttgctat tgacgacagc 120
tatggttcac tgtccaccaa ccaaaactgt gctcagtacc gccaatattt ctcccttgag 180
gggtacaaag aggtgtccct agaagagatc cacgctgtgt aaaaatttta caaaaaggta 240
ttgactttcc ctacagggtg tgtaataatt taattacagg cgggggcaac cccgcctgtt 300
ctgcagagga ggaatatagc catggaagtg aaaatcttca acacccagga tgttcaggat 360
tttctgcgtg ttgcaagcgg tctggaacaa gagggtggta atccgcgtgt taaacaaatt 420
attcatcgtg ttctgagcga cctgtataaa gcaattgaag atctgaatat caccagcgac 480
gaatattggg caggcgttgc atatctgaat cagctgggtg caaatcaaga agcaggtctg 540
ctgagtccgg gtctgggttt tgatcattat ctggatatgc gtatggatgc agaagatgca 600
gcactgggta ttgaaaatgc aacaccgcgt accattgaag gtccgctgta tgttgcgggt 660
gcaccggaaa gcgttggtta tgcacgcatg gatgatggta gcgatccgaa tggtcatacc 720
ctgattctgc atggcaccat ttttgatgca gatggtaaac cgctgccgaa tgcaaaagtt 780
gaaatttggc atgcaaacac caaaggcttt tatagccatt ttgatccgac cggtgaacag 840
caggccttta atatgcgtcg tagcattatt accgatgaga atggtcagta tcgtgttcgt 900
accattctgc ctgccggtta tggttgtcct ccggaaggtc cgacccagca actgctgaac 960
caactgggtc gtcatggtaa tcgtccggca catattcatt attttgttag cgcagatggt 1020
caccgtaaac tgaccaccca gattaatgtt gccggtgatc cgtataccta tgatgatttt 1080
gcatatgcca cccgtgaagg tctggttgtt gatgcagttg aacataccga tccggaagca 1140
attaaagcca atgatgtgga aggtcctttt gccgaaatgg tgtttgatct gaaactgacc 1200
cgtctggttg atggtgttga taatcaggtt gtggatcgtc cgcgtctggc agtttaatac 1260
accaaaatgg ttcaaaatta tcaggcgagt gatcatgatc actggcctgt ttttatttca 1320
gggaagggtg gagacaatta cgtggataat cagatcatcc aagaaaccgt ggataaaatt 1380
ctgagcgttc tgccgaatca ggcaggtcag ctggcacgtc tggtgcgtct gatgcaattt 1440
gcatgcgatc cgaccattac cgttattggc aaatataacc atggtaaaag ccgtctgctg 1500
aatgaactga ttggcaccga tatctttagc gttgcagata aacgtgaaac cattcagctg 1560
gccgaacata aacaggatca ggttcgttgg ctggatgcac ctggtctgga tgccgatgtt 1620
gcagcagttg atgatcgtca tgcatttgaa gcagtttgga cccaggcaga tattcgtctg 1680
tttgttcata gcgttcgtga aggtgaactg gatgcaaccg aacaccatct gctgcaacag 1740
ctgattgaag atgccgatca tagccgtcgt cagaccattc tggttctgac ccagattgat 1800
cagattccgg atcagaccat cctgacacag attaaaacca gcattgcaca gcaggttccg 1860
aaactggata tttgggcagt tagcgcaacc cgtcatcgtc agggcattga aaacggtaaa 1920
accctgctga tcgaaaaaag cggtattggt gcactgcgcc ataccctgga acaggcactg 1980
gcacaggtgc cgagcgcacg tacctatgaa aaaaatcgtc tgctgtcaga tctgcaccat 2040
cagctgaaac aactgctgct ggatcagaaa catgttctgc aacaactgca acagacacag 2100
caacagcagc tgcatgattt tgataccggt ctgattaaca ttctggacaa aattcgtgtt 2160
gatctggaac cgattgtgaa tattgatggt caggatcaag cactgaatcc ggatagcttt 2220
gcaaccatgt ttaaaaacac cgcagcaaaa cagcagcgtg ccaaagttca gattgcatat 2280
agccgtgcat gcattgaaat caacagccat ctgattcgcc atggtgttgt tggtctgcct 2340
gcggaacagc agaccaccat taaaagcatt gataccgtga ttgttgccgt gtttggtatc 2400
agcgttaaat ttcgtgatca gctgcgtgcc ctgttttata ccgataccga acgtcagcgt 2460
ctgcaacgtg aatttcgttt ctattttgaa aaaagtgccg gtcgcatgat tctggcagca 2520
aaaattgaac agaccatgcg tcagcagggc tgtattcaga atgccatgat ggcactgcaa 2580
caaatggaaa gcgcagcata aaaacacgga cgccgcaaac ggcgtccgaa tttcttggtc 2640
gaccgttaaa tctatcaccg caagggataa atatctaaca ccgtgcgtgt tgactatttt 2700
acctctggcg gtgataatgg ttgcatgtac taatctagat aaggaatata gccatgaccg 2760
caccgattca ggatctgcgt gatgcaattg ccctgctgca acagcatgat aatcagtatc 2820
tggaaaccga tcatccggtt gatccgaatg cagaactggc aggcgtttat cgtcatattg 2880
gtgccggtgg caccgttaaa cgtccgaccc gtattggtcc ggcaatgatg tttaataaca 2940
ttaaaggtta tccgcacagc cgtattctgg ttggtatgca tgcaagccgt cagcgtgcag 3000
cactgctgct gggttgtgaa gcaagtcagc tggcactgga agttggtaaa gcagttaaaa 3060
aaccggttgc accggtggtt gttccggcaa gcagcgcacc gtgtcaagag cagatttttc 3120
tggcagatga tccggatttt gatctgcgta ccctgctgcc tgcacatacc aataccccga 3180
ttgatgcagg tccgtttttt tgtctgggtc tggccctggc aagcgatccg gtggatgcaa 3240
gcctgaccga tgttaccatt catcgtctgt gtgttcaggg tcgtgatgaa ctgagcatgt 3300
tcctggcagc aggtcgccat attgaagttt ttcgtcagaa agcagaagca gcaggtaaac 3360
cgctgccgat taccattaat atgggtctgg acccagcaat ctatattggc gcatgttttg 3420
aagcaccgac caccccgttt ggttataatg aactgggtgt tgccggtgca ctgcgtcagc 3480
gtccggttga actggttcag ggtgttagcg ttccggaaaa agcaattgca cgtgccgaaa 3540
ttgttattga aggtgaactg ctgcctggtg ttcgtgttcg tgaagatcag cataccaatt 3600
caggtcatgc aatgccggaa tttccgggtt attgtggtgg tgcaaatccg agcctgccgg 3660
ttattaaagt taaagccgtt accatgcgca ataacgcaat tctgcaaacc ctggttggtc 3720
cgggtgaaga acataccacc ctggcaggtc tgccgaccga agcaagcatt tggaatgcag 3780
ttgaagcagc aattccgggt tttctgcaaa atgtttatgc ccataccgca ggcggtggta 3840
aatttctggg tattctgcaa gtgaaaaaac gtcagcctgc cgatgaaggt cgtcagggtc 3900
aggcagccct gctggcgctg gcaacctata gcgaactgaa aaatatcatt ctggtggatg 3960
aggatgtgga catttttgat agtgatgata ttctgtgggc aatgaccacc cgtatgcagg 4020
gtgatgttag cattaccacc attccgggta ttcgcggtca tcagctggac ccgagccaga 4080
caccggaata ttcaccgagc attcgtggta atggtattag ctgcaaaacc atctttgatt 4140
gtaccgttcc gtgggcactg aaaagccatt ttgaacgtgc accgtttgca gatgttgatc 4200
cgcgtccgtt tgcacctgaa tattttgcac gtctggaaaa aaatcagggc agcgcaaaat 4260
aagctaataa caggcctgct ggtaatcgca ggaattttta tttggatgga tccgcctacc 4320
tagcttccaa gaaagatatc ctaacagcac aagagcggaa agatgttttg ttctacatcc 4380
agaacaacct ctgctaaaat tcctgaaaaa ttttgcaaaa agttgttgac tttatctaca 4440
aggtgtggta taataatctt aacaacagca ggacgctccc gggttgagga aaacctaatg 4500
aaactgacca gcctgcgtgt tagcctgctg gcactgggtc tggttaccag cggttttgca 4560
gcagcagaaa cctataccgt tgatcgttat caggatgata gcgaaaaagg tagcctgcgt 4620
tgggcaattg aacagagcaa tgcaaatagc gcacaagaaa accagattct gattcaggca 4680
gttggtaaag caccgtatgt tatcaaagtt gataaaccgc tgcctccgat taaaagcagc 4740
gttaaaatca ttggcaccga gtgggataaa accggtgaat ttattgcaat tgatggcagc 4800
aactatatca aaggcgaagg tgaaaaagca tgtccgggtg caaatccggg tcagtatggc 4860
accaatgttc gtaccatgac cctgcctggt ctggttctgc aagatgttaa tggtgttacc 4920
ctgaaaggtc tggatgttca tcgtttttgt attggtgttc tggttaatcg cagcagcaat 4980
aacctgattc agcataatcg tatcagcaac aattatggtg gtgccggtgt tatgattacc 5040
ggtgatgatg gtaaaggtaa tccgaccagc accaccacca ataataacaa agttctggat 5100
aacgtgttca tcgataatgg tgatggtctg gaactgaccc gtggtgcagc atttaatctg 5160
attgcaaata acctgtttac cagcacaaaa gccaatccgg aaccgagcca gggtattgaa 5220
attctgtggg gtaatgataa tgccgtggtg ggtaacaaat tcgaaaacta ttcagatggc 5280
ctgcaaatca attggggtaa acgtaactat atcgcctata acgaactgac caataacagc 5340
ctgggtttca atctgacagg tgatggtaac attttcgaca gcaataaagt gcatggtaac 5400
cgtattggta ttgccattcg tagtgaaaaa gatgccaatg cacgtattac cctgaccaaa 5460
aatcagattt gggataacgg caaagatatc aaacgttgtg aagccggtgg tagctgtgtt 5520
ccgaatcagc gtctgggtgc aattgttttt ggtgttccgg cactggaaca tgaaggtttt 5580
gttggtagcc gtggcggtgg tgttgttatt gaaccggcaa aactgcaaaa aacctgcacc 5640
cagccgaacc agcagaattg taatgcaatt cctaatcagg gtattcaggc accgaaactg 5700
acagttagca aaaaacagct gaccgttgaa gttaaaggca cccctaatca gcgttataat 5760
gtggaatttt ttggcaatcg taatgccagc agcagcgaag cagaacagta tctgggtagc 5820
attgttgttg ttaccgatca tcagggtctg gcaaaagcaa attgggctcc gaaagttagc 5880
atgccgagcg ttaccgcaaa tgtgacagat catctgggtg cgaccagcga actgagcagc 5940
gcagttaaaa tgcgttaaat gcatgcgcgc cgcgttcgcg cggcgctttt ttttggtact 6000
cagtagcgct gtctggcaac ataaacggcc ccttctgggc aatgccgatc agttaaggat 6060
tagttgaccg atccttaaac tgaggcacta taacggcttc 6100