CN106536536A8 - 无热量甜味剂 - Google Patents

无热量甜味剂 Download PDF

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Publication number
CN106536536A8
CN106536536A8 CN201580035507.3A CN201580035507A CN106536536A8 CN 106536536 A8 CN106536536 A8 CN 106536536A8 CN 201580035507 A CN201580035507 A CN 201580035507A CN 106536536 A8 CN106536536 A8 CN 106536536A8
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rebaudiodside
caloric sweetener
producing
discloses
glycosyl
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CN106536536B (zh
CN106536536A (zh
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G.毛
V.S.P.查图尔维杜拉
X.于
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Conagen Inc
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Conagen Inc
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
    • C07H15/20—Carbocyclic rings
    • C07H15/24—Condensed ring systems having three or more rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
    • C07H15/20—Carbocyclic rings
    • C07H15/24—Condensed ring systems having three or more rings
    • C07H15/256—Polyterpene radicals
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30—Artificial sweetening agents
    • A23L27/33—Artificial sweetening agents containing sugars or derivatives
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/1062—Sucrose synthase (2.4.1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01013—Sucrose synthase (2.4.1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01017—Glucuronosyltransferase (2.4.1.17)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Seasonings (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)

Abstract

本发明公开了被称为莱鲍迪苷D2的甜菊醇糖苷。莱鲍迪苷D2具有连接至无糖基甜菊醇的五个β-D-葡萄糖基单元。本发明还公开了用于生产莱鲍迪苷D2的方法、UDP-糖基转移酶融合酶以及用于生产莱鲍迪苷D和莱鲍迪苷E的方法。
CN201580035507.3A 2014-05-05 2015-05-05 无热量甜味剂 Active CN106536536B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14/269,435 2014-05-05
US14/269,435 US9522929B2 (en) 2014-05-05 2014-05-05 Non-caloric sweetener
PCT/US2015/029163 WO2015171555A1 (en) 2014-05-05 2015-05-05 A non-caloric sweetener

Publications (3)

Publication Number Publication Date
CN106536536A CN106536536A (zh) 2017-03-22
CN106536536A8 true CN106536536A8 (zh) 2017-07-07
CN106536536B CN106536536B (zh) 2019-12-06

Family

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Family Applications (1)

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CN201580035507.3A Active CN106536536B (zh) 2014-05-05 2015-05-05 无热量甜味剂

Country Status (16)

Country Link
US (9) US9522929B2 (zh)
EP (3) EP4023660A1 (zh)
JP (1) JP6641357B2 (zh)
KR (1) KR102378988B1 (zh)
CN (1) CN106536536B (zh)
AR (1) AR100277A1 (zh)
AU (1) AU2015256232B2 (zh)
BR (1) BR112016025147B1 (zh)
CA (1) CA2982075A1 (zh)
DK (1) DK3140314T3 (zh)
ES (1) ES2750373T3 (zh)
HU (1) HUE045937T2 (zh)
MX (1) MX367883B (zh)
NZ (1) NZ725866A (zh)
WO (1) WO2015171555A1 (zh)
ZA (1) ZA201607592B (zh)

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US10597418B2 (en) 2020-03-24
JP2017519812A (ja) 2017-07-20
US10138263B2 (en) 2018-11-27
KR102378988B1 (ko) 2022-03-28
US9522929B2 (en) 2016-12-20
BR112016025147A2 (pt) 2017-12-12
AU2015256232B2 (en) 2019-09-26
US20200361978A1 (en) 2020-11-19
MX2016014506A (es) 2017-02-23
EP4023660A1 (en) 2022-07-06
ZA201607592B (en) 2019-02-27
EP3140314A1 (en) 2017-03-15
US10941174B2 (en) 2021-03-09
US20160208225A1 (en) 2016-07-21
EP3140314B1 (en) 2019-07-31
NZ725866A (en) 2022-11-25
KR20160147994A (ko) 2016-12-23
CN106536536B (zh) 2019-12-06
US20180244709A1 (en) 2018-08-30
US20150315623A1 (en) 2015-11-05
EP3140314A4 (en) 2017-10-18
DK3140314T3 (da) 2019-10-21
US20160207954A1 (en) 2016-07-21
CN106536536A (zh) 2017-03-22
US20180009835A1 (en) 2018-01-11
CA2982075A1 (en) 2015-11-12
EP3418288A1 (en) 2018-12-26
EP3418288B1 (en) 2022-01-19
US9988414B2 (en) 2018-06-05
US20180051049A1 (en) 2018-02-22
AU2015256232A1 (en) 2016-11-24
AR100277A1 (es) 2016-09-21
WO2015171555A1 (en) 2015-11-12
US10450339B2 (en) 2019-10-22
US9527880B2 (en) 2016-12-27
ES2750373T3 (es) 2020-03-25
JP6641357B2 (ja) 2020-02-05
US10654881B2 (en) 2020-05-19
BR112016025147B1 (pt) 2021-11-03
HUE045937T2 (hu) 2020-01-28
US20160208303A1 (en) 2016-07-21
MX367883B (es) 2019-09-10
US20180037600A1 (en) 2018-02-08
US9765104B2 (en) 2017-09-19

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