CN105209881B - 从细胞培养上清液和生物流体分离并纯化微泡的方法 - Google Patents
从细胞培养上清液和生物流体分离并纯化微泡的方法 Download PDFInfo
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- CN105209881B CN105209881B CN201480026884.6A CN201480026884A CN105209881B CN 105209881 B CN105209881 B CN 105209881B CN 201480026884 A CN201480026884 A CN 201480026884A CN 105209881 B CN105209881 B CN 105209881B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/14—Blood; Artificial blood
- A61K35/16—Blood plasma; Blood serum
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/22—Urine; Urinary tract, e.g. kidney or bladder; Intraglomerular mesangial cells; Renal mesenchymal cells; Adrenal gland
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/981—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of mammals or bird
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/981—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of mammals or bird
- A61K8/983—Blood, e.g. plasma
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- A—HUMAN NECESSITIES
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
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- A—HUMAN NECESSITIES
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
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- A—HUMAN NECESSITIES
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/06—Antianaemics
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- A—HUMAN NECESSITIES
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
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- A—HUMAN NECESSITIES
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/86—Products or compounds obtained by genetic engineering
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- Life Sciences & Earth Sciences (AREA)
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- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Cell Biology (AREA)
- Immunology (AREA)
- Zoology (AREA)
- Epidemiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Virology (AREA)
- Biotechnology (AREA)
- Dermatology (AREA)
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- Urology & Nephrology (AREA)
- Oncology (AREA)
- Diabetes (AREA)
- Cardiology (AREA)
- Heart & Thoracic Surgery (AREA)
- Neurosurgery (AREA)
- Neurology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Materials For Medical Uses (AREA)
- Cosmetics (AREA)
- Peptides Or Proteins (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110013769.7A CN112795533A (zh) | 2013-03-13 | 2014-03-12 | 从细胞培养上清液和生物流体分离并纯化微泡的方法 |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361778591P | 2013-03-13 | 2013-03-13 | |
| US61/778,591 | 2013-03-13 | ||
| PCT/US2014/024629 WO2014159662A1 (en) | 2013-03-13 | 2014-03-12 | Method for isolation and purification of microvesicles from cell culture supernatants and biological fluids |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110013769.7A Division CN112795533A (zh) | 2013-03-13 | 2014-03-12 | 从细胞培养上清液和生物流体分离并纯化微泡的方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105209881A CN105209881A (zh) | 2015-12-30 |
| CN105209881B true CN105209881B (zh) | 2021-01-22 |
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Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201480026884.6A Active CN105209881B (zh) | 2013-03-13 | 2014-03-12 | 从细胞培养上清液和生物流体分离并纯化微泡的方法 |
| CN202110013769.7A Pending CN112795533A (zh) | 2013-03-13 | 2014-03-12 | 从细胞培养上清液和生物流体分离并纯化微泡的方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110013769.7A Pending CN112795533A (zh) | 2013-03-13 | 2014-03-12 | 从细胞培养上清液和生物流体分离并纯化微泡的方法 |
Country Status (13)
| Country | Link |
|---|---|
| US (3) | US10500231B2 (enExample) |
| EP (3) | EP2972193B1 (enExample) |
| JP (3) | JP6562897B2 (enExample) |
| CN (2) | CN105209881B (enExample) |
| AU (4) | AU2014244618A1 (enExample) |
| CA (1) | CA2906502A1 (enExample) |
| DK (2) | DK2972193T3 (enExample) |
| ES (2) | ES2949637T3 (enExample) |
| FI (1) | FI3677271T3 (enExample) |
| HU (2) | HUE049003T2 (enExample) |
| PL (2) | PL2972193T3 (enExample) |
| PT (2) | PT2972193T (enExample) |
| WO (1) | WO2014159662A1 (enExample) |
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| US10640464B2 (en) | 2011-01-03 | 2020-05-05 | The William M. Yarbrough Foundation | Use of isothiocyanate functional surfactants as Nrf2 inducers to treat epidermolysis bullosa simplex and related diseases |
| HUE049003T2 (hu) | 2013-03-13 | 2020-09-28 | Univ Miami | Eljárás mikrovezikulumok izolálására és tisztítására sejttenyészet-felülúszóból és biológiai folyadékból |
| US20180325830A1 (en) | 2017-05-09 | 2018-11-15 | Vivex Biomedical, Inc. | Coated biological composition |
| US11160904B2 (en) | 2017-05-09 | 2021-11-02 | Vivex Biologies Group, Inc. | Biological composition in a protectant shroud and methods |
| US9687511B2 (en) | 2015-03-06 | 2017-06-27 | Vivex Biomedical, Inc. | Acellular biologic composition and method of manufacture |
| EP3267965A4 (en) * | 2015-03-11 | 2018-11-14 | Atta Behfar | Exosome delivery technology |
| WO2016149358A1 (en) * | 2015-03-16 | 2016-09-22 | Duncan Ross | Method of treatment comprising membrane-enclosed vesicle |
| WO2017023690A1 (en) * | 2015-07-31 | 2017-02-09 | Exoceuticals, Inc. | Exosome compositions and methods for preparation and use thereof for regulating and conditioning skin and hair |
| KR102418785B1 (ko) | 2015-09-25 | 2022-07-08 | (주)프로스테믹스 | 식물 착즙물 유래 세포 외 소포체를 포함하는 피부 개선 및 탈모 방지용 조성물 |
| KR102475127B1 (ko) * | 2015-09-30 | 2022-12-07 | (주)아모레퍼시픽 | 인삼 유래의 엑소좀 유사 베지클을 포함하는 탈모 방지 또는 육모 촉진용 조성물 |
| EP3397264A4 (en) * | 2015-12-30 | 2019-06-05 | The Regents of The University of California | METHOD FOR IMPROVED PREPARATION AND INSULATION OF CELL-DERIVED VESICLES |
| JP2019505590A (ja) * | 2016-01-19 | 2019-02-28 | ジェイ. サイレン、マッティ | 癌の予防、処置および/または緩和における使用のための方法および組成物 |
| KR102475129B1 (ko) * | 2016-03-22 | 2022-12-07 | (주)아모레퍼시픽 | 인삼 유래의 엑소좀 유사 베지클을 유효성분으로 포함하는 항노화용 조성물 |
| WO2018039100A2 (en) * | 2016-08-23 | 2018-03-01 | Albert Einstein College Of Medicine, Inc. | Stem cell-produced microvesicles for treating tendon pathologies |
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| JP2020528766A (ja) * | 2017-07-26 | 2020-10-01 | ロゼッタ エクソソーム | 陽イオンを利用した細胞外小胞体の分離方法 |
| ES2968625T3 (es) * | 2017-07-27 | 2024-05-13 | Univ Cornell | Un método para la fijación y retención de vesículas extracelulares, vesículas extracelulares fijadas y un método para diagnosticar una enfermedad |
| AU2018335788B2 (en) * | 2017-09-22 | 2024-08-15 | University Of Miami | Methods and compositions for the treatment of epidermolysis bullosa |
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| CA3089825C (en) * | 2018-01-29 | 2022-08-30 | Forever Labs, Inc. | System and method for isolating extracellular vesicles |
| CN108414334A (zh) * | 2018-02-23 | 2018-08-17 | 中山大学附属第医院 | 一种从精液中分离提纯外泌体的方法 |
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| CA3131922A1 (en) | 2019-02-28 | 2020-09-03 | William Samuel Fagg, Iv | Amniotic fluid-derived extracellular vesicles and uses thereof for wound healing |
| IT201900024580A1 (it) * | 2019-12-18 | 2021-06-18 | Consiglio Nazionale Ricerche | Vescicole extracellulari da microalghe |
| CN113384597A (zh) * | 2020-03-13 | 2021-09-14 | 西比曼生物科技(上海)有限公司 | 含人体细胞衍生的细胞膜外囊泡的雾化吸入制剂、制法及其应用 |
| CN111893093A (zh) * | 2020-08-03 | 2020-11-06 | 沈阳三禾生物科技有限公司 | 一种脐带间充质干细胞来源的超微因子的制备方法 |
| WO2022040516A1 (en) | 2020-08-21 | 2022-02-24 | University Of Miami | Compositions and methods of treatment using microvesicles from bone marrow-derived mesenchymal stem cells |
| EP4230724A4 (en) * | 2020-10-16 | 2024-05-22 | FUJIFILM Corporation | CULTURE MEDIUM FOR THE PRODUCTION OF EXTRACELLULAR VESICLES, CULTURE MEDIUM KIT, ADDITIVE AND METHOD FOR THE PRODUCTION OF EXTRACELLULAR VESICLES |
| CN114081900B (zh) * | 2021-02-24 | 2024-11-22 | 济宁医学院附属医院 | 间充质干细胞来源的纳米微囊泡在制备治疗子痫前期的药物中的应用 |
| CN115212230B (zh) * | 2021-04-21 | 2024-12-27 | 时比曼生物科技(上海)有限公司 | 含有干细胞胞外囊泡的药物组合物及其在呼吸道炎症治疗中的应用 |
| US20230390341A1 (en) | 2022-04-19 | 2023-12-07 | University Of Miami | Methods and compositions for prevention and treatment of graft versus host disease |
| KR20240028586A (ko) * | 2022-08-24 | 2024-03-05 | (주) 테라베스트 | 인간 세포 유래 미세소포체 및 히알루론산 또는 콜라겐을 포함하는 조성물 및 이의 용도 |
| CN116898781B (zh) * | 2023-02-07 | 2025-08-15 | 中国中医科学院中医基础理论研究所 | 大麦苗外泌体在制备具有抗衰老修护作用的产品中的应用 |
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