KR100519384B1 - 유전자 이입을 이용한 엑소좀의 제조방법 및 상기 엑소좀의 용도 - Google Patents
유전자 이입을 이용한 엑소좀의 제조방법 및 상기 엑소좀의 용도 Download PDFInfo
- Publication number
- KR100519384B1 KR100519384B1 KR10-2002-0047779A KR20020047779A KR100519384B1 KR 100519384 B1 KR100519384 B1 KR 100519384B1 KR 20020047779 A KR20020047779 A KR 20020047779A KR 100519384 B1 KR100519384 B1 KR 100519384B1
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- South Korea
- Prior art keywords
- exosomes
- tumor
- cells
- muc1
- present
- Prior art date
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- 210000001808 exosome Anatomy 0.000 title claims abstract description 64
- 238000004519 manufacturing process Methods 0.000 title abstract description 5
- 238000012637 gene transfection Methods 0.000 title 1
- 210000004027 cell Anatomy 0.000 claims abstract description 65
- 108091007433 antigens Proteins 0.000 claims abstract description 49
- 102000036639 antigens Human genes 0.000 claims abstract description 48
- 239000000427 antigen Substances 0.000 claims abstract description 46
- 206010028980 Neoplasm Diseases 0.000 claims abstract description 27
- 210000004881 tumor cell Anatomy 0.000 claims abstract description 16
- 108090000623 proteins and genes Proteins 0.000 claims description 31
- 230000037361 pathway Effects 0.000 claims description 4
- 230000003248 secreting effect Effects 0.000 claims description 4
- 229940022399 cancer vaccine Drugs 0.000 claims 1
- 238000009566 cancer vaccine Methods 0.000 claims 1
- 238000001890 transfection Methods 0.000 abstract description 7
- 238000002619 cancer immunotherapy Methods 0.000 abstract description 2
- 239000003795 chemical substances by application Substances 0.000 abstract description 2
- 102000004169 proteins and genes Human genes 0.000 description 25
- 101100346932 Mus musculus Muc1 gene Proteins 0.000 description 24
- 238000004458 analytical method Methods 0.000 description 11
- 238000001262 western blot Methods 0.000 description 10
- 238000002474 experimental method Methods 0.000 description 9
- 238000000034 method Methods 0.000 description 9
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- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 7
- 239000012528 membrane Substances 0.000 description 6
- 241000700605 Viruses Species 0.000 description 4
- 210000000170 cell membrane Anatomy 0.000 description 4
- 230000028993 immune response Effects 0.000 description 4
- 210000000987 immune system Anatomy 0.000 description 4
- 239000002245 particle Substances 0.000 description 4
- 238000012413 Fluorescence activated cell sorting analysis Methods 0.000 description 3
- 210000001744 T-lymphocyte Anatomy 0.000 description 3
- 210000002472 endoplasmic reticulum Anatomy 0.000 description 3
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- 230000008569 process Effects 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 229960005486 vaccine Drugs 0.000 description 3
- 102000002812 Heat-Shock Proteins Human genes 0.000 description 2
- 108010004889 Heat-Shock Proteins Proteins 0.000 description 2
- 101100346929 Homo sapiens MUC1 gene Proteins 0.000 description 2
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 2
- 108700018351 Major Histocompatibility Complex Proteins 0.000 description 2
- 102000018697 Membrane Proteins Human genes 0.000 description 2
- 108010052285 Membrane Proteins Proteins 0.000 description 2
- 210000000612 antigen-presenting cell Anatomy 0.000 description 2
- 230000000890 antigenic effect Effects 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 210000003743 erythrocyte Anatomy 0.000 description 2
- 239000007850 fluorescent dye Substances 0.000 description 2
- BRZYSWJRSDMWLG-CAXSIQPQSA-N geneticin Chemical compound O1C[C@@](O)(C)[C@H](NC)[C@@H](O)[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](C(C)O)O2)N)[C@@H](N)C[C@H]1N BRZYSWJRSDMWLG-CAXSIQPQSA-N 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 210000002487 multivesicular body Anatomy 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 230000020382 suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I Effects 0.000 description 2
- 208000023275 Autoimmune disease Diseases 0.000 description 1
- 206010006187 Breast cancer Diseases 0.000 description 1
- 208000026310 Breast neoplasm Diseases 0.000 description 1
- 101710163595 Chaperone protein DnaK Proteins 0.000 description 1
- 206010009944 Colon cancer Diseases 0.000 description 1
- 101710178376 Heat shock 70 kDa protein Proteins 0.000 description 1
- 101710152018 Heat shock cognate 70 kDa protein Proteins 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 102000043129 MHC class I family Human genes 0.000 description 1
- 108091054437 MHC class I family Proteins 0.000 description 1
- 241001529936 Murinae Species 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 239000012083 RIPA buffer Substances 0.000 description 1
- 208000005718 Stomach Neoplasms Diseases 0.000 description 1
- 210000003719 b-lymphocyte Anatomy 0.000 description 1
- 210000001772 blood platelet Anatomy 0.000 description 1
- 239000007975 buffered saline Substances 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
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- 208000029742 colonic neoplasm Diseases 0.000 description 1
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- 210000004443 dendritic cell Anatomy 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 238000000684 flow cytometry Methods 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 206010017758 gastric cancer Diseases 0.000 description 1
- 210000002288 golgi apparatus Anatomy 0.000 description 1
- 210000002865 immune cell Anatomy 0.000 description 1
- 230000002163 immunogen Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 210000004020 intracellular membrane Anatomy 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
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- 201000011549 stomach cancer Diseases 0.000 description 1
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- 238000005199 ultracentrifugation Methods 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/0005—Vertebrate antigens
- A61K39/0011—Cancer antigens
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/515—Animal cells
- A61K2039/5152—Tumor cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/515—Animal cells
- A61K2039/5156—Animal cells expressing foreign proteins
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Medicinal Chemistry (AREA)
- Public Health (AREA)
- Chemical & Material Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Mycology (AREA)
- Epidemiology (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Oncology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
Description
Claims (6)
- 삭제
- 삭제
- 삭제
- 삭제
- 삭제
- 1) 종양 특이항원에 대한 유전자가 종양 세포주 내에 도입되어 상기 종양 특이항원이 안정되게 발현되는 단계;2) 상기 발현된 종양 특이항원이 구조의 분비경로에 따라 종양 세포주 표면으로 노출되는 단계; 및3) 상기 종양 세포주 표면에 발현된 종양 특이항원이 종양 세포주의 엑소좀에 내재되어 세포 밖으로 방출되는 단계를 통해 제조되는 종양 특이 항원이 내재된 엑소좀을 이용한 것을 특징으로 하는 암백신용 엑소좀.
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR10-2002-0047779A KR100519384B1 (ko) | 2002-08-13 | 2002-08-13 | 유전자 이입을 이용한 엑소좀의 제조방법 및 상기 엑소좀의 용도 |
PCT/KR2003/001575 WO2004014954A1 (en) | 2002-08-13 | 2003-08-06 | Exosome containing exogenous antigen through gene transfection and method for utilizing the same |
AU2003248150A AU2003248150A1 (en) | 2002-08-13 | 2003-08-06 | Exosome containing exogenous antigen through gene transfection and method for utilizing the same |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR10-2002-0047779A KR100519384B1 (ko) | 2002-08-13 | 2002-08-13 | 유전자 이입을 이용한 엑소좀의 제조방법 및 상기 엑소좀의 용도 |
Publications (2)
Publication Number | Publication Date |
---|---|
KR20040015508A KR20040015508A (ko) | 2004-02-19 |
KR100519384B1 true KR100519384B1 (ko) | 2005-10-06 |
Family
ID=31713115
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR10-2002-0047779A KR100519384B1 (ko) | 2002-08-13 | 2002-08-13 | 유전자 이입을 이용한 엑소좀의 제조방법 및 상기 엑소좀의 용도 |
Country Status (3)
Country | Link |
---|---|
KR (1) | KR100519384B1 (ko) |
AU (1) | AU2003248150A1 (ko) |
WO (1) | WO2004014954A1 (ko) |
Families Citing this family (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1322115C (zh) * | 2005-07-06 | 2007-06-20 | 清华大学 | 载有外源配体分子的胞外体及其制备方法与应用 |
GB2437727B (en) * | 2006-05-04 | 2011-04-20 | Univ Open | Aptamers directed to MUC1 |
CN102497887A (zh) | 2009-04-17 | 2012-06-13 | Isis创新公司 | 递送遗传物质的组合物 |
ES2362589B1 (es) | 2009-12-28 | 2012-05-16 | Centre De Recerca En Salut Internacional De Barcelona | Exosomas derivados de reticulocitos infectados con plasmodium sp., método para su obtención y su uso. |
GB201121070D0 (en) | 2011-12-07 | 2012-01-18 | Isis Innovation | composition for delivery of biotherapeutics |
CN112795533A (zh) | 2013-03-13 | 2021-05-14 | 迈阿密大学 | 从细胞培养上清液和生物流体分离并纯化微泡的方法 |
CA2905081C (en) | 2013-03-15 | 2023-10-03 | Beth Israel Deaconess Medical Center, Inc. | Mirna biogenesis in exosomes for diagnosis and therapy |
SI3076949T1 (sl) | 2013-12-04 | 2020-01-31 | Board Of Regents, The University Of Texas System | Postopek za izoliranje eksosomov, ki izvirajo iz rakavih celic |
AU2016275046B2 (en) | 2015-06-10 | 2022-07-28 | Board Of Regents, The University Of Texas System | Use of exosomes for the treatment of disease |
JP2017101012A (ja) * | 2015-11-30 | 2017-06-08 | 義之 小山 | 免疫治療製剤 |
JP2019528674A (ja) | 2016-09-30 | 2019-10-17 | セレックス ライフ サイエンシズ,インコーポレーテッド | タンパク質をロードしたエキソソームを含む組成物、並びにその調製及び送達のための方法 |
US20220072049A1 (en) | 2020-08-21 | 2022-03-10 | University Of Miami | Compositions and methods of treatment using microvesicles from bone marrow-derived mesenchymal stem cells |
JP2024512691A (ja) | 2021-03-30 | 2024-03-19 | カイノス・メディスン・インコーポレイテッド | Fas関連因子1(faf1)内包エクソソームおよび抗癌剤としてのその使用 |
WO2023205158A1 (en) | 2022-04-19 | 2023-10-26 | University Of Miami | Compositions comprising microvesicles for use in the prevention and treatment of graft versus host disease |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1997005900A1 (en) * | 1995-08-03 | 1997-02-20 | Rijksuniversiteit Te Leiden | Cell derived antigen presenting vesicles |
WO2000028001A1 (fr) * | 1998-11-05 | 2000-05-18 | Institut National De La Sante Et De La Recherche Medicale | Exosomes modifies et utilisations |
WO2001082958A2 (en) * | 2000-04-27 | 2001-11-08 | Anosys, Inc. | Methods of producing membrane vesicles |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2766205B1 (fr) * | 1997-07-16 | 2002-08-30 | Inst Nat Sante Rech Med | Nouveau procede de sensibilisation de cellules presentatrices d'antigene et nouveaux moyens pour la mise en oeuvre du procede |
-
2002
- 2002-08-13 KR KR10-2002-0047779A patent/KR100519384B1/ko active IP Right Grant
-
2003
- 2003-08-06 AU AU2003248150A patent/AU2003248150A1/en not_active Abandoned
- 2003-08-06 WO PCT/KR2003/001575 patent/WO2004014954A1/en not_active Application Discontinuation
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1997005900A1 (en) * | 1995-08-03 | 1997-02-20 | Rijksuniversiteit Te Leiden | Cell derived antigen presenting vesicles |
WO2000028001A1 (fr) * | 1998-11-05 | 2000-05-18 | Institut National De La Sante Et De La Recherche Medicale | Exosomes modifies et utilisations |
WO2001082958A2 (en) * | 2000-04-27 | 2001-11-08 | Anosys, Inc. | Methods of producing membrane vesicles |
Non-Patent Citations (5)
Title |
---|
J Cell Biol. 1999 Nov 1;147(3):599-610 * |
J Cell Sci. 2000 Oct;113 Pt 19:3365-74 * |
J Immunol Methods. 2001 Jan 1;247(1-2):163-74 * |
LANCET, vol.360, pp.295-305(2002.7.27 * |
Lancet. 2002 Jul 27;360(9329):295-305 * |
Also Published As
Publication number | Publication date |
---|---|
WO2004014954A1 (en) | 2004-02-19 |
KR20040015508A (ko) | 2004-02-19 |
AU2003248150A1 (en) | 2004-02-25 |
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