CN105073995A - 增强的转基因表达和加工 - Google Patents

增强的转基因表达和加工 Download PDF

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Publication number
CN105073995A
CN105073995A CN201480010305.9A CN201480010305A CN105073995A CN 105073995 A CN105073995 A CN 105073995A CN 201480010305 A CN201480010305 A CN 201480010305A CN 105073995 A CN105073995 A CN 105073995A
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mar
nucleic acid
expression
protein
tep
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V·勒富尔
N·梅尔莫
A·雷加梅
M·巴赛特
D·莱伊
N·哈拉奇
K·科斯特拉科
P-A·吉拉
D·卡拉布列兹
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Selexis SA
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Selexis SA
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/241—Tumor Necrosis Factors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/106—Plasmid DNA for vertebrates
    • C12N2800/107—Plasmid DNA for vertebrates for mammalian
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/90—Vectors containing a transposable element
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/46—Vector systems having a special element relevant for transcription elements influencing chromatin structure, e.g. scaffold/matrix attachment region, methylation free island

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Oncology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Peptides Or Proteins (AREA)
CN201480010305.9A 2013-02-01 2014-02-01 增强的转基因表达和加工 Pending CN105073995A (zh)

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US201361759802P 2013-02-01 2013-02-01
US61/759,802 2013-02-01
US201361800244P 2013-03-15 2013-03-15
US61/800,244 2013-03-15
US201361806634P 2013-03-29 2013-03-29
US61/806,634 2013-03-29
PCT/IB2014/000100 WO2014118619A2 (en) 2013-02-01 2014-02-01 Enhanced transgene expression and processing

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EP (2) EP3536797B1 (https=)
JP (1) JP6563816B2 (https=)
KR (1) KR102235603B1 (https=)
CN (2) CN120718957A (https=)
AU (2) AU2014210856B2 (https=)
CA (1) CA2898878C (https=)
DK (2) DK2951309T3 (https=)
ES (2) ES2717175T3 (https=)
FI (1) FI3536797T3 (https=)
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PL (2) PL2951309T3 (https=)
PT (2) PT2951309T (https=)
RU (1) RU2711505C9 (https=)
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CN108504692A (zh) * 2018-03-26 2018-09-07 安徽大学 一种基因敲除cho细胞株的构建方法及其在治疗性重组蛋白表达中的应用
CN109071601A (zh) * 2016-03-14 2018-12-21 安吉拉·安娜·梅西纳 药学活性肽化合物，其制备方法和用途
CN109072257A (zh) * 2016-03-15 2018-12-21 马克思-德布鲁克-分子医学中心亥姆霍兹联合会 增强的睡美人转座子、试剂盒和转座方法
CN109312360A (zh) * 2016-03-15 2019-02-05 马克思-德布鲁克-分子医学中心亥姆霍兹联合会 用于原代细胞的基于转座子的转染系统
CN109415748A (zh) * 2016-05-03 2019-03-01 隆扎有限公司 用于蛋白生产的脂质代谢的调节
CN109576269A (zh) * 2014-04-01 2019-04-05 三生国健药业（上海）股份有限公司 一种新型的含mar核心片段的动物细胞表达载体
CN109790539A (zh) * 2016-10-03 2019-05-21 第一三共株式会社 Hspa5基因的启动子
CN111465697A (zh) * 2017-09-29 2020-07-28 贝特基因 用于预防或治疗心力衰竭的药物组合物
CN111500629A (zh) * 2020-04-17 2020-08-07 安徽中盛溯源生物科技有限公司 一种高表达层粘连蛋白-511变体的方法及其应用
CN113166767A (zh) * 2018-09-05 2021-07-23 马克思-德布鲁克-分子医学中心亥姆霍兹联合会 用于工程合成顺式调控dna的方法
CN113557297A (zh) * 2019-02-18 2021-10-26 帕多瓦大学 具有抗肿瘤活性的肽
CN113881703A (zh) * 2021-10-11 2022-01-04 中国人民解放军军事科学院军事医学研究院 一种提高cho细胞同源重组效率的方法及其相关产品和应用
CN114958914A (zh) * 2022-06-06 2022-08-30 新乡医学院 一种高效人类及哺乳动物细胞附着体表达载体、构建方法和应用
CN116440269A (zh) * 2022-01-10 2023-07-18 华中农业大学 Slc35a1基因作为靶点在防治猪流行性腹泻病中的应用

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CN105154473B (zh) * 2015-09-30 2019-03-01 上海细胞治疗研究院 一种高效安全的转座子整合系统及其用途
JP6871679B2 (ja) * 2016-03-23 2021-05-12 国立大学法人 岡山大学 遺伝子発現用カセット及びその産生物
JP2017070224A (ja) * 2015-10-06 2017-04-13 国立大学法人 岡山大学 遺伝子発現用カセット及びその産生物
US20180265894A1 (en) * 2015-10-06 2018-09-20 National University Corporation Okayama University Gene Expression Cassette And Product Thereof
IL259221B (en) 2015-12-24 2022-09-01 Selexis Sa Improved eukaryotic cells for protein production and methods for their production
US11278570B2 (en) 2016-12-16 2022-03-22 B-Mogen Biotechnologies, Inc. Enhanced hAT family transposon-mediated gene transfer and associated compositions, systems, and methods
JP7275043B2 (ja) 2016-12-16 2023-05-17 ビー－モーゲン・バイオテクノロジーズ，インコーポレーテッド 増大したｈＡＴファミリートランスポゾン媒介遺伝子導入ならびに関連する組成物、システムおよび方法
WO2018224162A1 (en) * 2017-06-09 2018-12-13 Biontech Rna Pharmaceuticals Gmbh Methods for characterizing loss of antigen presentation
CN109929839B (zh) * 2017-12-18 2021-02-12 华东师范大学 拆分型单碱基基因编辑系统及其应用
JP7407425B2 (ja) * 2018-05-18 2024-01-04 ソルボンヌ・ユニヴェルシテ 導入遺伝子組込みおよびそれらの転位依存性発現のための分子ツールおよび方法
CA3104288A1 (en) 2018-06-21 2019-12-26 B-Mogen Biotechnologies, Inc. Enhanced hat family transposon-mediated gene transfer and associated compositions, systems, and methods
RU2686102C1 (ru) * 2018-08-07 2019-04-24 Федеральное Государственное Бюджетное Учреждение Науки Институт Молекулярной Биологии Им. В.А. Энгельгардта Российской Академии Наук (Имб Ран) Рекомбинантный вектор для создания плазмидных генетических конструкций, обладающих повышенной длительностью экспрессии целевых генов
US20210380661A1 (en) * 2018-10-16 2021-12-09 Texas Tech University System Method to Express, Purify, and Biotinylate Eukaryotic Cell-Derived Major Histocompatibility Complexes
US20230193341A1 (en) 2018-10-24 2023-06-22 Selexis Sa Expression systems, recombinant cells and uses thereof
AU2019398101B2 (en) 2018-12-10 2025-12-18 Amgen Inc. Mutated piggybac transposase
EP3902910A1 (en) * 2018-12-24 2021-11-03 Selexis S.A. Characterization and inactivation of endogenous retroviruses in chinese hamster ovary cells
US20220088158A1 (en) 2019-01-23 2022-03-24 Aceragen, Inc. Method of ameliorating a pro-inflammatory immunophenotype in farber disease subjects by repeated administration of a recombinant human acid ceramidase
CN114401769A (zh) * 2019-03-27 2022-04-26 菲尼克斯组织修复公司 生产胶原蛋白7组合物的系统和方法
CN110006866B (zh) * 2019-04-16 2022-04-22 杭州迈尔德生物科技有限公司 一种阿片类活性物质的通用检测方法及其检测试剂盒
WO2020228721A1 (en) * 2019-05-13 2020-11-19 Lin Mei Chun Method and kit for monitoring cancer
CA3154452A1 (en) * 2019-10-10 2021-04-15 The Regents Of The University Of California Methods to stabilize mammalian cells
IL270306A (en) 2019-10-30 2021-05-31 Yeda Res & Dev Prevention and treatment of pre-myeloid and myeloid malignancies
CN110863008B (zh) * 2019-11-22 2020-10-16 昆明学院 Mar序列调控弱启动子构建高效表达载体的方法
WO2021130718A1 (en) 2019-12-24 2021-07-01 Selexis Sa Targeted integration in mammalian sequences enhancing gene expression
KR102937659B1 (ko) * 2020-02-19 2026-03-10 우시 바이올로직스 아일랜드 리미티드 강화된 발현 시스템 및 그 사용방법
WO2021231569A1 (en) * 2020-05-14 2021-11-18 Seattle Children's Hospital (dba Seattle Children's Research Institute) Dual promoter systems
US12173304B2 (en) * 2020-07-08 2024-12-24 Neuracle Genetics Inc. Intron fragments
CN115896227A (zh) * 2022-12-20 2023-04-04 西北农林科技大学 一种鉴定外源基因整合位点以增强转基因表达的方法
GB202305637D0 (en) 2023-04-17 2023-05-31 Macomics Ltd Transposons, vectors and genetically engineered cells

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Cited By (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109576269B (zh) * 2014-04-01 2020-08-28 三生国健药业（上海）股份有限公司 一种含mar核心片段的动物细胞表达载体
CN109576269A (zh) * 2014-04-01 2019-04-05 三生国健药业（上海）股份有限公司 一种新型的含mar核心片段的动物细胞表达载体
CN109071601A (zh) * 2016-03-14 2018-12-21 安吉拉·安娜·梅西纳 药学活性肽化合物，其制备方法和用途
CN109072257A (zh) * 2016-03-15 2018-12-21 马克思-德布鲁克-分子医学中心亥姆霍兹联合会 增强的睡美人转座子、试剂盒和转座方法
CN109312360A (zh) * 2016-03-15 2019-02-05 马克思-德布鲁克-分子医学中心亥姆霍兹联合会 用于原代细胞的基于转座子的转染系统
CN109312360B (zh) * 2016-03-15 2022-10-28 马克思-德布鲁克-分子医学中心亥姆霍兹联合会 用于原代细胞的基于转座子的转染系统
CN109415748A (zh) * 2016-05-03 2019-03-01 隆扎有限公司 用于蛋白生产的脂质代谢的调节
US11555204B2 (en) 2016-10-03 2023-01-17 Daiichi Sankyo Company, Limited Promoter of Hspa5 gene
CN109790539A (zh) * 2016-10-03 2019-05-21 第一三共株式会社 Hspa5基因的启动子
TWI812596B (zh) * 2016-10-03 2023-08-21 日商第一三共股份有限公司 Hspa5基因的啓動子
CN111465697A (zh) * 2017-09-29 2020-07-28 贝特基因 用于预防或治疗心力衰竭的药物组合物
CN111465697B (zh) * 2017-09-29 2023-10-13 贝特基因 用于预防或治疗心力衰竭的药物组合物
CN108504692B (zh) * 2018-03-26 2021-07-23 安徽大学 一种基因敲除cho细胞株的构建方法及其在治疗性重组蛋白表达中的应用
CN108504692A (zh) * 2018-03-26 2018-09-07 安徽大学 一种基因敲除cho细胞株的构建方法及其在治疗性重组蛋白表达中的应用
CN113166767A (zh) * 2018-09-05 2021-07-23 马克思-德布鲁克-分子医学中心亥姆霍兹联合会 用于工程合成顺式调控dna的方法
CN113557297A (zh) * 2019-02-18 2021-10-26 帕多瓦大学 具有抗肿瘤活性的肽
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Application publication date: 20151118