RU2711505C9 - Улучшенные экспрессия и процессинг трансгена - Google Patents

Улучшенные экспрессия и процессинг трансгена Download PDF

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RU2711505C9
RU2711505C9 RU2015127667A RU2015127667A RU2711505C9 RU 2711505 C9 RU2711505 C9 RU 2711505C9 RU 2015127667 A RU2015127667 A RU 2015127667A RU 2015127667 A RU2015127667 A RU 2015127667A RU 2711505 C9 RU2711505 C9 RU 2711505C9
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mar
seq
transgene
cell
expression
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RU2015127667A (ru
RU2711505C2 (ru
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ФОРН Валери ЛЯ
Николас МЕРМО
Александр РЕГАМИ
Монтс БЮСЕТА
Дебора ЛЭЙ
Ниам ХАРРАГИ
Кая КОСТИРКО
Пьер-Ален ЖИРО
Давид КАЛАБРЕС
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Селексис С.А.
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/241—Tumor Necrosis Factors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/106—Plasmid DNA for vertebrates
    • C12N2800/107—Plasmid DNA for vertebrates for mammalian
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/90—Vectors containing a transposable element
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/46—Vector systems having a special element relevant for transcription elements influencing chromatin structure, e.g. scaffold/matrix attachment region, methylation free island

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Oncology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Peptides Or Proteins (AREA)
RU2015127667A 2013-02-01 2014-02-01 Улучшенные экспрессия и процессинг трансгена RU2711505C9 (ru)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201361759802P 2013-02-01 2013-02-01
US61/759,802 2013-02-01
US201361800244P 2013-03-15 2013-03-15
US61/800,244 2013-03-15
US201361806634P 2013-03-29 2013-03-29
US61/806,634 2013-03-29
PCT/IB2014/000100 WO2014118619A2 (en) 2013-02-01 2014-02-01 Enhanced transgene expression and processing

Related Child Applications (1)

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RU2019139048A Division RU2808756C1 (ru) 2013-02-01 2014-02-01 Улучшенные экспрессия и процессинг трансгена

Publications (3)

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RU2015127667A RU2015127667A (ru) 2017-03-06
RU2711505C2 RU2711505C2 (ru) 2020-01-17
RU2711505C9 true RU2711505C9 (ru) 2020-08-12

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EP (2) EP3536797B1 (https=)
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KR (1) KR102235603B1 (https=)
CN (2) CN120718957A (https=)
AU (2) AU2014210856B2 (https=)
CA (1) CA2898878C (https=)
DK (2) DK2951309T3 (https=)
ES (2) ES2717175T3 (https=)
FI (1) FI3536797T3 (https=)
HU (2) HUE061494T2 (https=)
IL (1) IL240198B (https=)
LT (1) LT2951309T (https=)
PL (2) PL2951309T3 (https=)
PT (2) PT2951309T (https=)
RU (1) RU2711505C9 (https=)
SG (2) SG10201803758XA (https=)
SI (1) SI2951309T1 (https=)
WO (1) WO2014118619A2 (https=)
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Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109576269B (zh) * 2014-04-01 2020-08-28 三生国健药业（上海）股份有限公司 一种含mar核心片段的动物细胞表达载体
CN105154473B (zh) * 2015-09-30 2019-03-01 上海细胞治疗研究院 一种高效安全的转座子整合系统及其用途
JP6871679B2 (ja) * 2016-03-23 2021-05-12 国立大学法人 岡山大学 遺伝子発現用カセット及びその産生物
JP2017070224A (ja) * 2015-10-06 2017-04-13 国立大学法人 岡山大学 遺伝子発現用カセット及びその産生物
US20180265894A1 (en) * 2015-10-06 2018-09-20 National University Corporation Okayama University Gene Expression Cassette And Product Thereof
IL259221B (en) 2015-12-24 2022-09-01 Selexis Sa Improved eukaryotic cells for protein production and methods for their production
ITUA20161610A1 (it) * 2016-03-14 2017-09-14 Angela Anna Messina Composto peptidico farmacologicamente attivo, procedimento per la sua preparazione e suo uso.
EP3219800A1 (en) * 2016-03-15 2017-09-20 Max-Delbrück-Centrum Für Molekulare Medizin A transposon-based transfection system for primary cells
EP3219803A1 (en) * 2016-03-15 2017-09-20 Max-Delbrück-Centrum für Molekulare Medizin Enhanced sleeping beauty transposons, kits and methods of transposition
WO2017191165A1 (en) * 2016-05-03 2017-11-09 Lonza Ltd Modulation of lipid metabolism for protein production
SG11201902998SA (en) * 2016-10-03 2019-05-30 Daiichi Sankyo Co Ltd PROMOTER OF Hspa5 GENE
US11278570B2 (en) 2016-12-16 2022-03-22 B-Mogen Biotechnologies, Inc. Enhanced hAT family transposon-mediated gene transfer and associated compositions, systems, and methods
JP7275043B2 (ja) 2016-12-16 2023-05-17 ビー－モーゲン・バイオテクノロジーズ，インコーポレーテッド 増大したｈＡＴファミリートランスポゾン媒介遺伝子導入ならびに関連する組成物、システムおよび方法
WO2018224162A1 (en) * 2017-06-09 2018-12-13 Biontech Rna Pharmaceuticals Gmbh Methods for characterizing loss of antigen presentation
CN111465697B (zh) * 2017-09-29 2023-10-13 贝特基因 用于预防或治疗心力衰竭的药物组合物
CN109929839B (zh) * 2017-12-18 2021-02-12 华东师范大学 拆分型单碱基基因编辑系统及其应用
CN108504692B (zh) * 2018-03-26 2021-07-23 安徽大学 一种基因敲除cho细胞株的构建方法及其在治疗性重组蛋白表达中的应用
JP7407425B2 (ja) * 2018-05-18 2024-01-04 ソルボンヌ・ユニヴェルシテ 導入遺伝子組込みおよびそれらの転位依存性発現のための分子ツールおよび方法
CA3104288A1 (en) 2018-06-21 2019-12-26 B-Mogen Biotechnologies, Inc. Enhanced hat family transposon-mediated gene transfer and associated compositions, systems, and methods
RU2686102C1 (ru) * 2018-08-07 2019-04-24 Федеральное Государственное Бюджетное Учреждение Науки Институт Молекулярной Биологии Им. В.А. Энгельгардта Российской Академии Наук (Имб Ран) Рекомбинантный вектор для создания плазмидных генетических конструкций, обладающих повышенной длительностью экспрессии целевых генов
US20210343368A1 (en) * 2018-09-05 2021-11-04 Max-Delbrück-Centrum Für Molekulare Medizin In Der Helmholtz-Gemeinschaft Method for Engineering Synthetic Cis-Regulatory DNA
US20210380661A1 (en) * 2018-10-16 2021-12-09 Texas Tech University System Method to Express, Purify, and Biotinylate Eukaryotic Cell-Derived Major Histocompatibility Complexes
US20230193341A1 (en) 2018-10-24 2023-06-22 Selexis Sa Expression systems, recombinant cells and uses thereof
AU2019398101B2 (en) 2018-12-10 2025-12-18 Amgen Inc. Mutated piggybac transposase
EP3902910A1 (en) * 2018-12-24 2021-11-03 Selexis S.A. Characterization and inactivation of endogenous retroviruses in chinese hamster ovary cells
US20220088158A1 (en) 2019-01-23 2022-03-24 Aceragen, Inc. Method of ameliorating a pro-inflammatory immunophenotype in farber disease subjects by repeated administration of a recombinant human acid ceramidase
IT201900002321A1 (it) * 2019-02-18 2020-08-18 Univ Degli Studi Padova Peptidi con attivita' antitumorale
CN114401769A (zh) * 2019-03-27 2022-04-26 菲尼克斯组织修复公司 生产胶原蛋白7组合物的系统和方法
CN110006866B (zh) * 2019-04-16 2022-04-22 杭州迈尔德生物科技有限公司 一种阿片类活性物质的通用检测方法及其检测试剂盒
WO2020228721A1 (en) * 2019-05-13 2020-11-19 Lin Mei Chun Method and kit for monitoring cancer
CA3154452A1 (en) * 2019-10-10 2021-04-15 The Regents Of The University Of California Methods to stabilize mammalian cells
IL270306A (en) 2019-10-30 2021-05-31 Yeda Res & Dev Prevention and treatment of pre-myeloid and myeloid malignancies
CN110863008B (zh) * 2019-11-22 2020-10-16 昆明学院 Mar序列调控弱启动子构建高效表达载体的方法
WO2021130718A1 (en) 2019-12-24 2021-07-01 Selexis Sa Targeted integration in mammalian sequences enhancing gene expression
KR102937659B1 (ko) * 2020-02-19 2026-03-10 우시 바이올로직스 아일랜드 리미티드 강화된 발현 시스템 및 그 사용방법
CN111500629B (zh) * 2020-04-17 2021-11-19 安徽中盛溯源生物科技有限公司 一种高表达层粘连蛋白-511变体的方法及其应用
WO2021231569A1 (en) * 2020-05-14 2021-11-18 Seattle Children's Hospital (dba Seattle Children's Research Institute) Dual promoter systems
US12173304B2 (en) * 2020-07-08 2024-12-24 Neuracle Genetics Inc. Intron fragments
CN113881703B (zh) * 2021-10-11 2022-06-21 中国人民解放军军事科学院军事医学研究院 一种提高cho细胞同源重组效率的方法及其相关产品和应用
CN116440269B (zh) * 2022-01-10 2024-02-09 华中农业大学 Slc35a1基因作为靶点在防治猪流行性腹泻病中的应用
CN114958914B (zh) * 2022-06-06 2023-09-26 新乡医学院 一种人类及哺乳动物细胞附着体表达载体、构建方法和应用
CN115896227A (zh) * 2022-12-20 2023-04-04 西北农林科技大学 一种鉴定外源基因整合位点以增强转基因表达的方法
GB202305637D0 (en) 2023-04-17 2023-05-31 Macomics Ltd Transposons, vectors and genetically engineered cells

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0743365A2 (en) * 1995-05-17 1996-11-20 Ajinomoto Co., Inc. Process for efficiently producing transglutaminase through DNA recombination
US20040235011A1 (en) * 2002-06-26 2004-11-25 Cooper Richard K. Production of multimeric proteins
WO2005010046A1 (en) * 2003-06-27 2005-02-03 Bayer Pharmaceuticals Corporation Use of molecular chaperones for the enhanced production of secreted, recombinant proteins in mammalian cells
RU2394104C2 (ru) * 2004-05-20 2010-07-10 Проекто Де Биомедисина Сима, С.Л. Аденовирусный/альфа-вирусный гибридный вектор для эффективного введения и экспрессии терапевтических генов в опухолевые клетки
WO2011033375A2 (en) * 2009-09-18 2011-03-24 Selexis S.A. Products and methods for enhanced transgene expression and processing

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5731178A (en) 1990-03-21 1998-03-24 Behringwerke Aktiengesellschaft Attachment-elements for stimulation of eukaryotic expression systems
DK1395669T3 (da) 2001-01-26 2009-11-16 Selexis Sa Matriks bindingsregioner og fremgangsmåder til anvendelse af disse
US20040203158A1 (en) * 2003-01-15 2004-10-14 Hackett Perry B. Transposon-insulator element delivery systems
KR101269656B1 (ko) 2003-10-24 2013-05-30 셀렉시스 에스. 에이. Ｍａｒ 서열의 복합 트랜스펙션 방법에 의한 포유동물 세포에서의 고효율 유전자 전달 및 발현
JP2008543283A (ja) * 2005-06-10 2008-12-04 ジェネンテック・インコーポレーテッド インスレーターエレメントを含むベクターを使用する抗体発現の改良
CA2715078C (en) * 2007-09-26 2019-07-23 Intrexon Corporation Synthetic 5'utrs, expression vectors, and methods for increasing transgene expression
US20090247609A1 (en) 2007-12-20 2009-10-01 Hitto Kaufmann Sm-protein based secretion engineering
WO2010008564A2 (en) * 2008-07-16 2010-01-21 Recombinetics Plaice dna transposon system
US9150881B2 (en) * 2009-04-09 2015-10-06 Proteovec Holding, L.L.C. Production of proteins using transposon-based vectors

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0743365A2 (en) * 1995-05-17 1996-11-20 Ajinomoto Co., Inc. Process for efficiently producing transglutaminase through DNA recombination
US20040235011A1 (en) * 2002-06-26 2004-11-25 Cooper Richard K. Production of multimeric proteins
WO2005010046A1 (en) * 2003-06-27 2005-02-03 Bayer Pharmaceuticals Corporation Use of molecular chaperones for the enhanced production of secreted, recombinant proteins in mammalian cells
RU2394104C2 (ru) * 2004-05-20 2010-07-10 Проекто Де Биомедисина Сима, С.Л. Аденовирусный/альфа-вирусный гибридный вектор для эффективного введения и экспрессии терапевтических генов в опухолевые клетки
WO2011033375A2 (en) * 2009-09-18 2011-03-24 Selexis S.A. Products and methods for enhanced transgene expression and processing

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
MATASCI M. et al. CHO cell lines generated by PiggyBac transposition, BMC PROCEEDINGS, 2011, v. 5, p. P31. *

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SG11201505487UA (en) 2015-08-28
AU2019250224A1 (en) 2019-11-07
JP2016504922A (ja) 2016-02-18
CN105073995A (zh) 2015-11-18
SG10201803758XA (en) 2018-06-28
HUE043103T2 (hu) 2019-08-28
US11898154B2 (en) 2024-02-13
WO2014118619A2 (en) 2014-08-07
IL240198A0 (en) 2015-09-24
US20150361451A1 (en) 2015-12-17
JP6563816B2 (ja) 2019-08-21
HUE061494T2 (hu) 2023-07-28
CN120718957A (zh) 2025-09-30
PL2951309T3 (pl) 2019-06-28
CA2898878A1 (en) 2014-08-07
DK3536797T3 (da) 2023-04-03
ES2717175T3 (es) 2019-06-19
AU2019250224B2 (en) 2022-05-12
DK2951309T3 (en) 2019-04-23
KR20150123803A (ko) 2015-11-04
RU2015127667A (ru) 2017-03-06
CA2898878C (en) 2020-04-28
KR102235603B1 (ko) 2021-04-05
RU2711505C2 (ru) 2020-01-17
LT2951309T (lt) 2019-03-25
IL240198B (en) 2019-10-31
ZA201506419B (en) 2017-02-22
FI3536797T3 (fi) 2023-03-23
AU2014210856B2 (en) 2019-10-24
PL3536797T3 (pl) 2023-09-11
EP3536797B1 (en) 2023-01-04
PT2951309T (pt) 2019-04-03
EP3536797A1 (en) 2019-09-11
EP2951309A2 (en) 2015-12-09
HK1216323A1 (en) 2016-11-04
PT3536797T (pt) 2023-03-15
WO2014118619A3 (en) 2014-10-30
EP2951309B1 (en) 2019-01-30
AU2014210856A1 (en) 2015-07-30
DK3536797T5 (da) 2024-09-02
ES2940433T3 (es) 2023-05-08
SI2951309T1 (sl) 2019-04-30

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