CN104903465B - 基于寡核苷酸切割及延伸‑依赖性非‑杂交探测的靶核酸序列的检测 - Google Patents
基于寡核苷酸切割及延伸‑依赖性非‑杂交探测的靶核酸序列的检测 Download PDFInfo
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- CN104903465B CN104903465B CN201380068241.3A CN201380068241A CN104903465B CN 104903465 B CN104903465 B CN 104903465B CN 201380068241 A CN201380068241 A CN 201380068241A CN 104903465 B CN104903465 B CN 104903465B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6823—Release of bound markers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR10-2012-0154834 | 2012-12-27 | ||
| KR20120154834 | 2012-12-27 | ||
| KR20130008580 | 2013-01-25 | ||
| KR10-2013-0008580 | 2013-01-25 | ||
| KR20130034670 | 2013-03-29 | ||
| KR10-2013-0034670 | 2013-03-29 | ||
| PCT/KR2013/012312 WO2014104818A1 (en) | 2012-12-27 | 2013-12-27 | Detection of target nucleic acid sequence by pto cleavage and extension-dependent non-hybridization assay |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104903465A CN104903465A (zh) | 2015-09-09 |
| CN104903465B true CN104903465B (zh) | 2017-04-26 |
Family
ID=51021756
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201380068241.3A Active CN104903465B (zh) | 2012-12-27 | 2013-12-27 | 基于寡核苷酸切割及延伸‑依赖性非‑杂交探测的靶核酸序列的检测 |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US9783845B2 (enExample) |
| EP (1) | EP2943586B1 (enExample) |
| JP (1) | JP6178430B2 (enExample) |
| KR (1) | KR101760267B1 (enExample) |
| CN (1) | CN104903465B (enExample) |
| AU (1) | AU2013371128B2 (enExample) |
| BR (1) | BR112015014152B1 (enExample) |
| CA (1) | CA2896213C (enExample) |
| ES (1) | ES2653642T3 (enExample) |
| MX (1) | MX361191B (enExample) |
| RU (1) | RU2620958C2 (enExample) |
| WO (1) | WO2014104818A1 (enExample) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102218776B1 (ko) | 2016-06-10 | 2021-02-22 | 주식회사 씨젠 | 태깅 올리고뉴클레오타이드의 제공 방법 |
| KR102264865B1 (ko) | 2016-06-30 | 2021-06-14 | 주식회사 씨젠 | 반응 분석용 형광 검출 장치 |
| KR102239375B1 (ko) | 2016-10-06 | 2021-04-13 | 주식회사 씨젠 | 시료 내 타겟 핵산분자의 검출에 이용되는 올리고뉴클레오타이드의 제공방법 |
| BR112019012925A2 (pt) * | 2016-12-23 | 2019-12-10 | Albert-Ludwigs-Universität Freiburg | sonda mediadora em duas partes |
| KR102651626B1 (ko) | 2016-12-29 | 2024-03-26 | 주식회사 씨젠 | 프라이머 다이머 형성을 감소시키고 증폭 효율을 증가시키는 방법 |
| CN110475871B (zh) | 2017-03-28 | 2024-04-12 | Seegene株式会社 | 用于确定靶核酸序列的存在的分析信号 |
| CN108823287B (zh) | 2017-04-28 | 2019-04-12 | 厦门大学 | 一种检测靶核酸序列的方法 |
| CN107236815A (zh) * | 2017-07-18 | 2017-10-10 | 江西贤聚景欣医药生物科技有限公司 | 用于靶核酸序列检测的多重淬灭荧光探针及方法 |
| US11591645B2 (en) | 2017-08-31 | 2023-02-28 | Seegene, Inc. | Evaluation of performance of components using a pair of dimer-forming primers |
| WO2019066461A2 (en) | 2017-09-29 | 2019-04-04 | Seegene, Inc. | DETECTION OF TARGET NUCLEIC ACID SEQUENCES BY CLEAVAGE ANALYSIS AND EXTENSION OF PTO |
| KR102369388B1 (ko) | 2018-01-05 | 2022-03-02 | 주식회사 씨젠 | 시료에서 M. tuberculosis, M. bovis 및 M. bovis BCG의 존재 또는 부존재를 결정하는 방법 |
| IL277870B2 (en) | 2018-04-20 | 2025-02-01 | Seegene Inc | Method and device for identifying diverse targets of nucleic acid sequences in a sample |
| US20220025449A1 (en) | 2018-12-14 | 2022-01-27 | Seegene, Inc. | Method for detecting a target analyte in a sample using an s-shaped function for a slope data set |
| EP3899035B1 (en) * | 2018-12-20 | 2025-04-16 | F. Hoffmann-La Roche AG | Detection of target nucleic acid by solid-phase molography |
| KR102894467B1 (ko) | 2021-03-05 | 2025-12-03 | 주식회사 씨젠 | SARS-CoV-2 변이를 검출하는 방법 |
| EP4403645A4 (en) * | 2021-09-17 | 2025-10-08 | Seegene Inc | DETECTION OF A TARGET NUCLEIC ACID SEQUENCE USING A MARKER OLIGONUCLEOTIDE CARRYING A SYNTHETIC NON-NATURAL BASE |
| EP4265734A1 (de) * | 2022-04-22 | 2023-10-25 | Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. | Nukleinsäurenachweis in einer pcr mittels eines zielsequenz-unspezifischem modularer reporterkomplexes |
| US20250340928A1 (en) * | 2022-04-22 | 2025-11-06 | Albert-Ludwigs-Universität Freiburg | Nucleic acid detection in a pcr by means of a target-sequence-unspecific modular reporter complex |
| EP4603593A1 (en) | 2022-10-14 | 2025-08-20 | Seegene, Inc. | Method for detecting target nucleic acid in sample using two probes with different tm values |
| US12643104B2 (en) | 2022-10-28 | 2026-06-02 | Seegene, Inc. | Cartridge for detecting target analyte |
| EP4372100A1 (en) * | 2022-11-15 | 2024-05-22 | Stilla Technologies | Data analysis method for multiplex digital pcr using color-combination |
| EP4621068A1 (en) | 2022-11-16 | 2025-09-24 | Seegene, Inc. | Detection of target nucleic acid by quenching-pto cleavage and extension (q-ptoce) assay |
| KR20260003722A (ko) * | 2023-06-30 | 2026-01-07 | 주식회사 씨젠 | Hto 절단 및 자기-연장 어세이에 의해 샘플 내의 타겟 핵산을 검출하는 방법 |
| WO2025143555A1 (ko) | 2023-12-26 | 2025-07-03 | 주식회사 씨젠 | 샘플 내 타겟 분석물질을 검출하는 방법 |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101076537A (zh) * | 2003-11-19 | 2007-11-21 | 阿莱洛吉克生物科学公司 | 标记有多个荧光团的寡核苷酸 |
| US20090023151A1 (en) * | 2006-08-30 | 2009-01-22 | Dawson Elliott P | Method For The Labeling And Detection Of Small Polynucleotides |
| CN101506384A (zh) * | 2006-07-17 | 2009-08-12 | 布兰迪斯大学 | 专一性寡核苷酸和其在核酸扩增与检测中的用途 |
| US20100129812A1 (en) * | 2001-01-27 | 2010-05-27 | Jae Chern Yoo | Nucleic acid hybridization essay method |
| WO2012096523A2 (en) * | 2011-01-11 | 2012-07-19 | Seegene, Inc. | Detection of target nucleic acid sequences by pto cleavage and extension assay |
| WO2012134195A2 (en) * | 2011-03-29 | 2012-10-04 | Seegene, Inc. | Detection of target nucleic acid sequence by pto cleavage and extension-dependent cleavage |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| US5994069A (en) | 1996-01-24 | 1999-11-30 | Third Wave Technologies, Inc. | Detection of nucleic acids by multiple sequential invasive cleavages |
| US5541311A (en) | 1992-12-07 | 1996-07-30 | Third Wave Technologies, Inc. | Nucleic acid encoding synthesis-deficient thermostable DNA polymerase |
| US5538848A (en) | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
| GB9812768D0 (en) | 1998-06-13 | 1998-08-12 | Zeneca Ltd | Methods |
| US7381532B2 (en) | 1999-10-29 | 2008-06-03 | Stratagene California | Compositions and methods for the detection of a nucleic acid using a cleavage reaction |
| US6893819B1 (en) | 2000-11-21 | 2005-05-17 | Stratagene California | Methods for detection of a nucleic acid by sequential amplification |
| US7601497B2 (en) * | 2000-06-15 | 2009-10-13 | Qiagen Gaithersburg, Inc. | Detection of nucleic acids by target-specific hybrid capture method |
| US7309573B2 (en) | 2000-11-21 | 2007-12-18 | Stratagene California | Methods for detection of a nucleic acid by sequential amplification |
| GB0205455D0 (en) * | 2002-03-07 | 2002-04-24 | Molecular Sensing Plc | Nucleic acid probes, their synthesis and use |
| EP2118310B1 (en) | 2006-12-29 | 2013-03-06 | Applied Biosystems, LLC | Systems and methods for detecting nucleic acids |
| WO2012150749A1 (en) * | 2011-05-04 | 2012-11-08 | Seegene, Inc. | Detection of target nucleic acid sequences by po cleavage and hybridization |
-
2013
- 2013-12-27 AU AU2013371128A patent/AU2013371128B2/en active Active
- 2013-12-27 BR BR112015014152-8A patent/BR112015014152B1/pt active IP Right Grant
- 2013-12-27 CN CN201380068241.3A patent/CN104903465B/zh active Active
- 2013-12-27 ES ES13868457.6T patent/ES2653642T3/es active Active
- 2013-12-27 RU RU2015130335A patent/RU2620958C2/ru active
- 2013-12-27 MX MX2015008494A patent/MX361191B/es active IP Right Grant
- 2013-12-27 US US14/655,847 patent/US9783845B2/en active Active
- 2013-12-27 WO PCT/KR2013/012312 patent/WO2014104818A1/en not_active Ceased
- 2013-12-27 JP JP2015550327A patent/JP6178430B2/ja active Active
- 2013-12-27 EP EP13868457.6A patent/EP2943586B1/en active Active
- 2013-12-27 KR KR1020157017974A patent/KR101760267B1/ko active Active
- 2013-12-27 CA CA2896213A patent/CA2896213C/en active Active
-
2017
- 2017-09-07 US US15/697,617 patent/US20180057869A1/en not_active Abandoned
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100129812A1 (en) * | 2001-01-27 | 2010-05-27 | Jae Chern Yoo | Nucleic acid hybridization essay method |
| CN101076537A (zh) * | 2003-11-19 | 2007-11-21 | 阿莱洛吉克生物科学公司 | 标记有多个荧光团的寡核苷酸 |
| CN101506384A (zh) * | 2006-07-17 | 2009-08-12 | 布兰迪斯大学 | 专一性寡核苷酸和其在核酸扩增与检测中的用途 |
| US20090023151A1 (en) * | 2006-08-30 | 2009-01-22 | Dawson Elliott P | Method For The Labeling And Detection Of Small Polynucleotides |
| WO2012096523A2 (en) * | 2011-01-11 | 2012-07-19 | Seegene, Inc. | Detection of target nucleic acid sequences by pto cleavage and extension assay |
| WO2012134195A2 (en) * | 2011-03-29 | 2012-10-04 | Seegene, Inc. | Detection of target nucleic acid sequence by pto cleavage and extension-dependent cleavage |
Also Published As
| Publication number | Publication date |
|---|---|
| RU2620958C2 (ru) | 2017-05-30 |
| JP2016502861A (ja) | 2016-02-01 |
| EP2943586A1 (en) | 2015-11-18 |
| MX2015008494A (es) | 2015-09-10 |
| KR101760267B1 (ko) | 2017-07-24 |
| WO2014104818A1 (en) | 2014-07-03 |
| EP2943586B1 (en) | 2017-09-20 |
| CN104903465A (zh) | 2015-09-09 |
| CA2896213C (en) | 2018-12-11 |
| US20160060690A1 (en) | 2016-03-03 |
| AU2013371128A1 (en) | 2015-08-13 |
| AU2013371128B2 (en) | 2017-08-10 |
| US9783845B2 (en) | 2017-10-10 |
| JP6178430B2 (ja) | 2017-08-09 |
| ES2653642T3 (es) | 2018-02-08 |
| BR112015014152B1 (pt) | 2023-01-24 |
| EP2943586A4 (en) | 2016-09-28 |
| US20180057869A1 (en) | 2018-03-01 |
| BR112015014152A2 (enExample) | 2017-08-22 |
| CA2896213A1 (en) | 2014-07-03 |
| KR20150093766A (ko) | 2015-08-18 |
| MX361191B (es) | 2018-11-29 |
| RU2015130335A (ru) | 2017-01-30 |
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