AU2013371128B2 - Detection of target nucleic acid sequence by PTO Cleavage and Extension-Dependent Non-Hybridization assay - Google Patents
Detection of target nucleic acid sequence by PTO Cleavage and Extension-Dependent Non-Hybridization assay Download PDFInfo
- Publication number
- AU2013371128B2 AU2013371128B2 AU2013371128A AU2013371128A AU2013371128B2 AU 2013371128 B2 AU2013371128 B2 AU 2013371128B2 AU 2013371128 A AU2013371128 A AU 2013371128A AU 2013371128 A AU2013371128 A AU 2013371128A AU 2013371128 B2 AU2013371128 B2 AU 2013371128B2
- Authority
- AU
- Australia
- Prior art keywords
- cto
- nucleic acid
- pto
- target nucleic
- acid sequence
- Prior art date
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6823—Release of bound markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR10-2012-0154834 | 2012-12-27 | ||
| KR20120154834 | 2012-12-27 | ||
| KR20130008580 | 2013-01-25 | ||
| KR10-2013-0008580 | 2013-01-25 | ||
| KR20130034670 | 2013-03-29 | ||
| KR10-2013-0034670 | 2013-03-29 | ||
| PCT/KR2013/012312 WO2014104818A1 (en) | 2012-12-27 | 2013-12-27 | Detection of target nucleic acid sequence by pto cleavage and extension-dependent non-hybridization assay |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2013371128A1 AU2013371128A1 (en) | 2015-08-13 |
| AU2013371128B2 true AU2013371128B2 (en) | 2017-08-10 |
Family
ID=51021756
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2013371128A Active AU2013371128B2 (en) | 2012-12-27 | 2013-12-27 | Detection of target nucleic acid sequence by PTO Cleavage and Extension-Dependent Non-Hybridization assay |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US9783845B2 (enExample) |
| EP (1) | EP2943586B1 (enExample) |
| JP (1) | JP6178430B2 (enExample) |
| KR (1) | KR101760267B1 (enExample) |
| CN (1) | CN104903465B (enExample) |
| AU (1) | AU2013371128B2 (enExample) |
| BR (1) | BR112015014152B1 (enExample) |
| CA (1) | CA2896213C (enExample) |
| ES (1) | ES2653642T3 (enExample) |
| MX (1) | MX361191B (enExample) |
| RU (1) | RU2620958C2 (enExample) |
| WO (1) | WO2014104818A1 (enExample) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102218776B1 (ko) | 2016-06-10 | 2021-02-22 | 주식회사 씨젠 | 태깅 올리고뉴클레오타이드의 제공 방법 |
| KR102264865B1 (ko) | 2016-06-30 | 2021-06-14 | 주식회사 씨젠 | 반응 분석용 형광 검출 장치 |
| KR102239375B1 (ko) | 2016-10-06 | 2021-04-13 | 주식회사 씨젠 | 시료 내 타겟 핵산분자의 검출에 이용되는 올리고뉴클레오타이드의 제공방법 |
| BR112019012925A2 (pt) * | 2016-12-23 | 2019-12-10 | Albert-Ludwigs-Universität Freiburg | sonda mediadora em duas partes |
| KR102651626B1 (ko) | 2016-12-29 | 2024-03-26 | 주식회사 씨젠 | 프라이머 다이머 형성을 감소시키고 증폭 효율을 증가시키는 방법 |
| CN110475871B (zh) | 2017-03-28 | 2024-04-12 | Seegene株式会社 | 用于确定靶核酸序列的存在的分析信号 |
| CN108823287B (zh) | 2017-04-28 | 2019-04-12 | 厦门大学 | 一种检测靶核酸序列的方法 |
| CN107236815A (zh) * | 2017-07-18 | 2017-10-10 | 江西贤聚景欣医药生物科技有限公司 | 用于靶核酸序列检测的多重淬灭荧光探针及方法 |
| US11591645B2 (en) | 2017-08-31 | 2023-02-28 | Seegene, Inc. | Evaluation of performance of components using a pair of dimer-forming primers |
| WO2019066461A2 (en) | 2017-09-29 | 2019-04-04 | Seegene, Inc. | DETECTION OF TARGET NUCLEIC ACID SEQUENCES BY CLEAVAGE ANALYSIS AND EXTENSION OF PTO |
| KR102369388B1 (ko) | 2018-01-05 | 2022-03-02 | 주식회사 씨젠 | 시료에서 M. tuberculosis, M. bovis 및 M. bovis BCG의 존재 또는 부존재를 결정하는 방법 |
| IL277870B2 (en) | 2018-04-20 | 2025-02-01 | Seegene Inc | Method and device for identifying diverse targets of nucleic acid sequences in a sample |
| US20220025449A1 (en) | 2018-12-14 | 2022-01-27 | Seegene, Inc. | Method for detecting a target analyte in a sample using an s-shaped function for a slope data set |
| EP3899035B1 (en) * | 2018-12-20 | 2025-04-16 | F. Hoffmann-La Roche AG | Detection of target nucleic acid by solid-phase molography |
| KR102894467B1 (ko) | 2021-03-05 | 2025-12-03 | 주식회사 씨젠 | SARS-CoV-2 변이를 검출하는 방법 |
| EP4403645A4 (en) * | 2021-09-17 | 2025-10-08 | Seegene Inc | DETECTION OF A TARGET NUCLEIC ACID SEQUENCE USING A MARKER OLIGONUCLEOTIDE CARRYING A SYNTHETIC NON-NATURAL BASE |
| EP4265734A1 (de) * | 2022-04-22 | 2023-10-25 | Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. | Nukleinsäurenachweis in einer pcr mittels eines zielsequenz-unspezifischem modularer reporterkomplexes |
| US20250340928A1 (en) * | 2022-04-22 | 2025-11-06 | Albert-Ludwigs-Universität Freiburg | Nucleic acid detection in a pcr by means of a target-sequence-unspecific modular reporter complex |
| EP4603593A1 (en) | 2022-10-14 | 2025-08-20 | Seegene, Inc. | Method for detecting target nucleic acid in sample using two probes with different tm values |
| US12643104B2 (en) | 2022-10-28 | 2026-06-02 | Seegene, Inc. | Cartridge for detecting target analyte |
| EP4372100A1 (en) * | 2022-11-15 | 2024-05-22 | Stilla Technologies | Data analysis method for multiplex digital pcr using color-combination |
| EP4621068A1 (en) | 2022-11-16 | 2025-09-24 | Seegene, Inc. | Detection of target nucleic acid by quenching-pto cleavage and extension (q-ptoce) assay |
| KR20260003722A (ko) * | 2023-06-30 | 2026-01-07 | 주식회사 씨젠 | Hto 절단 및 자기-연장 어세이에 의해 샘플 내의 타겟 핵산을 검출하는 방법 |
| WO2025143555A1 (ko) | 2023-12-26 | 2025-07-03 | 주식회사 씨젠 | 샘플 내 타겟 분석물질을 검출하는 방법 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012096523A2 (en) * | 2011-01-11 | 2012-07-19 | Seegene, Inc. | Detection of target nucleic acid sequences by pto cleavage and extension assay |
| WO2012134195A2 (en) * | 2011-03-29 | 2012-10-04 | Seegene, Inc. | Detection of target nucleic acid sequence by pto cleavage and extension-dependent cleavage |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| US5994069A (en) | 1996-01-24 | 1999-11-30 | Third Wave Technologies, Inc. | Detection of nucleic acids by multiple sequential invasive cleavages |
| US5541311A (en) | 1992-12-07 | 1996-07-30 | Third Wave Technologies, Inc. | Nucleic acid encoding synthesis-deficient thermostable DNA polymerase |
| US5538848A (en) | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
| GB9812768D0 (en) | 1998-06-13 | 1998-08-12 | Zeneca Ltd | Methods |
| US7381532B2 (en) | 1999-10-29 | 2008-06-03 | Stratagene California | Compositions and methods for the detection of a nucleic acid using a cleavage reaction |
| US6893819B1 (en) | 2000-11-21 | 2005-05-17 | Stratagene California | Methods for detection of a nucleic acid by sequential amplification |
| US7601497B2 (en) * | 2000-06-15 | 2009-10-13 | Qiagen Gaithersburg, Inc. | Detection of nucleic acids by target-specific hybrid capture method |
| US7309573B2 (en) | 2000-11-21 | 2007-12-18 | Stratagene California | Methods for detection of a nucleic acid by sequential amplification |
| KR20020063359A (ko) * | 2001-01-27 | 2002-08-03 | 일렉트론 바이오 (주) | 핵산 및 올리거뉴클레오티드의 상보적 이중결합의 특정서열에 특이적으로 반응하는 절단기법을 이용한 핵산 혼성분석 방법 및 장치 |
| GB0205455D0 (en) * | 2002-03-07 | 2002-04-24 | Molecular Sensing Plc | Nucleic acid probes, their synthesis and use |
| US7667024B2 (en) * | 2003-11-19 | 2010-02-23 | Allelogic Biosciences Corp. | Oligonucleotides labeled with a plurality of fluorophores |
| JP5286261B2 (ja) * | 2006-07-17 | 2013-09-11 | ブランディーズ・ユニバーシティ | 特殊化オリゴヌクレオチドならびに核酸の増幅および検出でのその使用 |
| US20090023151A1 (en) | 2006-08-30 | 2009-01-22 | Dawson Elliott P | Method For The Labeling And Detection Of Small Polynucleotides |
| EP2118310B1 (en) | 2006-12-29 | 2013-03-06 | Applied Biosystems, LLC | Systems and methods for detecting nucleic acids |
| WO2012150749A1 (en) * | 2011-05-04 | 2012-11-08 | Seegene, Inc. | Detection of target nucleic acid sequences by po cleavage and hybridization |
-
2013
- 2013-12-27 AU AU2013371128A patent/AU2013371128B2/en active Active
- 2013-12-27 BR BR112015014152-8A patent/BR112015014152B1/pt active IP Right Grant
- 2013-12-27 CN CN201380068241.3A patent/CN104903465B/zh active Active
- 2013-12-27 ES ES13868457.6T patent/ES2653642T3/es active Active
- 2013-12-27 RU RU2015130335A patent/RU2620958C2/ru active
- 2013-12-27 MX MX2015008494A patent/MX361191B/es active IP Right Grant
- 2013-12-27 US US14/655,847 patent/US9783845B2/en active Active
- 2013-12-27 WO PCT/KR2013/012312 patent/WO2014104818A1/en not_active Ceased
- 2013-12-27 JP JP2015550327A patent/JP6178430B2/ja active Active
- 2013-12-27 EP EP13868457.6A patent/EP2943586B1/en active Active
- 2013-12-27 KR KR1020157017974A patent/KR101760267B1/ko active Active
- 2013-12-27 CA CA2896213A patent/CA2896213C/en active Active
-
2017
- 2017-09-07 US US15/697,617 patent/US20180057869A1/en not_active Abandoned
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012096523A2 (en) * | 2011-01-11 | 2012-07-19 | Seegene, Inc. | Detection of target nucleic acid sequences by pto cleavage and extension assay |
| WO2012134195A2 (en) * | 2011-03-29 | 2012-10-04 | Seegene, Inc. | Detection of target nucleic acid sequence by pto cleavage and extension-dependent cleavage |
Also Published As
| Publication number | Publication date |
|---|---|
| RU2620958C2 (ru) | 2017-05-30 |
| JP2016502861A (ja) | 2016-02-01 |
| CN104903465B (zh) | 2017-04-26 |
| EP2943586A1 (en) | 2015-11-18 |
| MX2015008494A (es) | 2015-09-10 |
| KR101760267B1 (ko) | 2017-07-24 |
| WO2014104818A1 (en) | 2014-07-03 |
| EP2943586B1 (en) | 2017-09-20 |
| CN104903465A (zh) | 2015-09-09 |
| CA2896213C (en) | 2018-12-11 |
| US20160060690A1 (en) | 2016-03-03 |
| AU2013371128A1 (en) | 2015-08-13 |
| US9783845B2 (en) | 2017-10-10 |
| JP6178430B2 (ja) | 2017-08-09 |
| ES2653642T3 (es) | 2018-02-08 |
| BR112015014152B1 (pt) | 2023-01-24 |
| EP2943586A4 (en) | 2016-09-28 |
| US20180057869A1 (en) | 2018-03-01 |
| BR112015014152A2 (enExample) | 2017-08-22 |
| CA2896213A1 (en) | 2014-07-03 |
| KR20150093766A (ko) | 2015-08-18 |
| MX361191B (es) | 2018-11-29 |
| RU2015130335A (ru) | 2017-01-30 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FGA | Letters patent sealed or granted (standard patent) |