RU2620958C2 - Детекция нуклеиновокислотной последовательности-мишени в анализе с отсутствием гибридизации, зависящим от расщепления и удлинения зондирующего и метящего олигонуклеотида (рто) - Google Patents

Детекция нуклеиновокислотной последовательности-мишени в анализе с отсутствием гибридизации, зависящим от расщепления и удлинения зондирующего и метящего олигонуклеотида (рто) Download PDF

Info

Publication number
RU2620958C2
RU2620958C2 RU2015130335A RU2015130335A RU2620958C2 RU 2620958 C2 RU2620958 C2 RU 2620958C2 RU 2015130335 A RU2015130335 A RU 2015130335A RU 2015130335 A RU2015130335 A RU 2015130335A RU 2620958 C2 RU2620958 C2 RU 2620958C2
Authority
RU
Russia
Prior art keywords
nucleic acid
pto
cto
region
acid sequence
Prior art date
Application number
RU2015130335A
Other languages
English (en)
Russian (ru)
Other versions
RU2015130335A (ru
Inventor
Джон Йоон ЧУН
Янг Джо ЛИ
Original Assignee
Сиджен, Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Сиджен, Инк. filed Critical Сиджен, Инк.
Publication of RU2015130335A publication Critical patent/RU2015130335A/ru
Application granted granted Critical
Publication of RU2620958C2 publication Critical patent/RU2620958C2/ru

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
RU2015130335A 2012-12-27 2013-12-27 Детекция нуклеиновокислотной последовательности-мишени в анализе с отсутствием гибридизации, зависящим от расщепления и удлинения зондирующего и метящего олигонуклеотида (рто) RU2620958C2 (ru)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
KR10-2012-0154834 2012-12-27
KR20120154834 2012-12-27
KR20130008580 2013-01-25
KR10-2013-0008580 2013-01-25
KR20130034670 2013-03-29
KR10-2013-0034670 2013-03-29
PCT/KR2013/012312 WO2014104818A1 (en) 2012-12-27 2013-12-27 Detection of target nucleic acid sequence by pto cleavage and extension-dependent non-hybridization assay

Publications (2)

Publication Number Publication Date
RU2015130335A RU2015130335A (ru) 2017-01-30
RU2620958C2 true RU2620958C2 (ru) 2017-05-30

Family

ID=51021756

Family Applications (1)

Application Number Title Priority Date Filing Date
RU2015130335A RU2620958C2 (ru) 2012-12-27 2013-12-27 Детекция нуклеиновокислотной последовательности-мишени в анализе с отсутствием гибридизации, зависящим от расщепления и удлинения зондирующего и метящего олигонуклеотида (рто)

Country Status (12)

Country Link
US (2) US9783845B2 (enExample)
EP (1) EP2943586B1 (enExample)
JP (1) JP6178430B2 (enExample)
KR (1) KR101760267B1 (enExample)
CN (1) CN104903465B (enExample)
AU (1) AU2013371128B2 (enExample)
BR (1) BR112015014152B1 (enExample)
CA (1) CA2896213C (enExample)
ES (1) ES2653642T3 (enExample)
MX (1) MX361191B (enExample)
RU (1) RU2620958C2 (enExample)
WO (1) WO2014104818A1 (enExample)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102218776B1 (ko) 2016-06-10 2021-02-22 주식회사 씨젠 태깅 올리고뉴클레오타이드의 제공 방법
KR102264865B1 (ko) 2016-06-30 2021-06-14 주식회사 씨젠 반응 분석용 형광 검출 장치
KR102239375B1 (ko) 2016-10-06 2021-04-13 주식회사 씨젠 시료 내 타겟 핵산분자의 검출에 이용되는 올리고뉴클레오타이드의 제공방법
BR112019012925A2 (pt) * 2016-12-23 2019-12-10 Albert-Ludwigs-Universität Freiburg sonda mediadora em duas partes
KR102651626B1 (ko) 2016-12-29 2024-03-26 주식회사 씨젠 프라이머 다이머 형성을 감소시키고 증폭 효율을 증가시키는 방법
CN110475871B (zh) 2017-03-28 2024-04-12 Seegene株式会社 用于确定靶核酸序列的存在的分析信号
CN108823287B (zh) 2017-04-28 2019-04-12 厦门大学 一种检测靶核酸序列的方法
CN107236815A (zh) * 2017-07-18 2017-10-10 江西贤聚景欣医药生物科技有限公司 用于靶核酸序列检测的多重淬灭荧光探针及方法
US11591645B2 (en) 2017-08-31 2023-02-28 Seegene, Inc. Evaluation of performance of components using a pair of dimer-forming primers
WO2019066461A2 (en) 2017-09-29 2019-04-04 Seegene, Inc. DETECTION OF TARGET NUCLEIC ACID SEQUENCES BY CLEAVAGE ANALYSIS AND EXTENSION OF PTO
KR102369388B1 (ko) 2018-01-05 2022-03-02 주식회사 씨젠 시료에서 M. tuberculosis, M. bovis 및 M. bovis BCG의 존재 또는 부존재를 결정하는 방법
IL277870B2 (en) 2018-04-20 2025-02-01 Seegene Inc Method and device for identifying diverse targets of nucleic acid sequences in a sample
US20220025449A1 (en) 2018-12-14 2022-01-27 Seegene, Inc. Method for detecting a target analyte in a sample using an s-shaped function for a slope data set
EP3899035B1 (en) * 2018-12-20 2025-04-16 F. Hoffmann-La Roche AG Detection of target nucleic acid by solid-phase molography
KR102894467B1 (ko) 2021-03-05 2025-12-03 주식회사 씨젠 SARS-CoV-2 변이를 검출하는 방법
EP4403645A4 (en) * 2021-09-17 2025-10-08 Seegene Inc DETECTION OF A TARGET NUCLEIC ACID SEQUENCE USING A MARKER OLIGONUCLEOTIDE CARRYING A SYNTHETIC NON-NATURAL BASE
EP4265734A1 (de) * 2022-04-22 2023-10-25 Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. Nukleinsäurenachweis in einer pcr mittels eines zielsequenz-unspezifischem modularer reporterkomplexes
US20250340928A1 (en) * 2022-04-22 2025-11-06 Albert-Ludwigs-Universität Freiburg Nucleic acid detection in a pcr by means of a target-sequence-unspecific modular reporter complex
EP4603593A1 (en) 2022-10-14 2025-08-20 Seegene, Inc. Method for detecting target nucleic acid in sample using two probes with different tm values
US12643104B2 (en) 2022-10-28 2026-06-02 Seegene, Inc. Cartridge for detecting target analyte
EP4372100A1 (en) * 2022-11-15 2024-05-22 Stilla Technologies Data analysis method for multiplex digital pcr using color-combination
EP4621068A1 (en) 2022-11-16 2025-09-24 Seegene, Inc. Detection of target nucleic acid by quenching-pto cleavage and extension (q-ptoce) assay
KR20260003722A (ko) * 2023-06-30 2026-01-07 주식회사 씨젠 Hto 절단 및 자기-연장 어세이에 의해 샘플 내의 타겟 핵산을 검출하는 방법
WO2025143555A1 (ko) 2023-12-26 2025-07-03 주식회사 씨젠 샘플 내 타겟 분석물질을 검출하는 방법

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050221315A1 (en) * 2002-03-07 2005-10-06 Helen Braven Nucleic acid probes, their synthesis and use
RU2437939C2 (ru) * 2005-11-07 2011-12-27 Киаджен Гейтерсбёрг, Сша Детекция нуклеиновых кислот способом, основанным на связывании мишенеспецифичного гибрида

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5994069A (en) 1996-01-24 1999-11-30 Third Wave Technologies, Inc. Detection of nucleic acids by multiple sequential invasive cleavages
US5541311A (en) 1992-12-07 1996-07-30 Third Wave Technologies, Inc. Nucleic acid encoding synthesis-deficient thermostable DNA polymerase
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
GB9812768D0 (en) 1998-06-13 1998-08-12 Zeneca Ltd Methods
US7381532B2 (en) 1999-10-29 2008-06-03 Stratagene California Compositions and methods for the detection of a nucleic acid using a cleavage reaction
US6893819B1 (en) 2000-11-21 2005-05-17 Stratagene California Methods for detection of a nucleic acid by sequential amplification
US7309573B2 (en) 2000-11-21 2007-12-18 Stratagene California Methods for detection of a nucleic acid by sequential amplification
KR20020063359A (ko) * 2001-01-27 2002-08-03 일렉트론 바이오 (주) 핵산 및 올리거뉴클레오티드의 상보적 이중결합의 특정서열에 특이적으로 반응하는 절단기법을 이용한 핵산 혼성분석 방법 및 장치
US7667024B2 (en) * 2003-11-19 2010-02-23 Allelogic Biosciences Corp. Oligonucleotides labeled with a plurality of fluorophores
JP5286261B2 (ja) * 2006-07-17 2013-09-11 ブランディーズ・ユニバーシティ 特殊化オリゴヌクレオチドならびに核酸の増幅および検出でのその使用
US20090023151A1 (en) 2006-08-30 2009-01-22 Dawson Elliott P Method For The Labeling And Detection Of Small Polynucleotides
EP2118310B1 (en) 2006-12-29 2013-03-06 Applied Biosystems, LLC Systems and methods for detecting nucleic acids
MX352460B (es) * 2011-01-11 2017-11-24 Seegene Inc Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto.
US11078525B2 (en) * 2011-03-29 2021-08-03 Seegene, Inc. Detection of target nucleic acid sequence by PTO cleavage and extension-dependent cleavage
WO2012150749A1 (en) * 2011-05-04 2012-11-08 Seegene, Inc. Detection of target nucleic acid sequences by po cleavage and hybridization

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050221315A1 (en) * 2002-03-07 2005-10-06 Helen Braven Nucleic acid probes, their synthesis and use
RU2437939C2 (ru) * 2005-11-07 2011-12-27 Киаджен Гейтерсбёрг, Сша Детекция нуклеиновых кислот способом, основанным на связывании мишенеспецифичного гибрида

Also Published As

Publication number Publication date
JP2016502861A (ja) 2016-02-01
CN104903465B (zh) 2017-04-26
EP2943586A1 (en) 2015-11-18
MX2015008494A (es) 2015-09-10
KR101760267B1 (ko) 2017-07-24
WO2014104818A1 (en) 2014-07-03
EP2943586B1 (en) 2017-09-20
CN104903465A (zh) 2015-09-09
CA2896213C (en) 2018-12-11
US20160060690A1 (en) 2016-03-03
AU2013371128A1 (en) 2015-08-13
AU2013371128B2 (en) 2017-08-10
US9783845B2 (en) 2017-10-10
JP6178430B2 (ja) 2017-08-09
ES2653642T3 (es) 2018-02-08
BR112015014152B1 (pt) 2023-01-24
EP2943586A4 (en) 2016-09-28
US20180057869A1 (en) 2018-03-01
BR112015014152A2 (enExample) 2017-08-22
CA2896213A1 (en) 2014-07-03
KR20150093766A (ko) 2015-08-18
MX361191B (es) 2018-11-29
RU2015130335A (ru) 2017-01-30

Similar Documents

Publication Publication Date Title
US12606862B2 (en) Detection of target nucleic acid sequences by PTO cleavage and extension assay
CA2896213C (en) Detection of target nucleic acid sequence by pto cleavage and extension-dependent non-hybridization assay
RU2608501C2 (ru) Детекция нуклеиновокислотной последовательности-мишени в анализе с гибридизацией сигнального олигонуклеотида, зависящей от расщепления и удлинения зондирующего и метящего олигонуклеотида (варианты)
RU2542478C2 (ru) Дискриминирующий мишень зонд, способ его конструирования и способ детекции нуклеиновокислотной последовательности-мишени (варианты)
RU2620955C2 (ru) Детекция нуклеотидной вариации в нуклеиновокислотной последовательности-мишени в анализе с расщеплением и удлинением зондирующего и метящего олигонуклеотида (рто)
WO2012150749A1 (en) Detection of target nucleic acid sequences by po cleavage and hybridization
CN105705657A (zh) 基于利用杂交-捕捉和模板化寡核苷酸的探测和标记寡核苷酸切割及延伸分析的在固相中的靶核酸序列检测
AU2015203677B2 (en) Detection of Target Nucleic Acid Sequences by PTO Cleavage and Extension
RU2588457C2 (ru) Определение целевых нуклеиновокислотных последовательностей путем расщепления зондирующего олигонуклеотида и гибридизации
WO2013187628A1 (en) Detection of target nucleic acid sequence by pto cleavage and extension-dependent transcription
NZ604338B2 (en) Detection of target nucleic acid sequences by pto cleavage and extension assay
NZ706144B2 (en) Detection of target nucleic acid sequences by pto cleavage and extension assay