BR112015014152B1 - Método e kit para detectar uma sequência de ácido nucleico alvo por ensaio de clivagem de pto e não hibridização dependente de extensão - Google Patents

Método e kit para detectar uma sequência de ácido nucleico alvo por ensaio de clivagem de pto e não hibridização dependente de extensão Download PDF

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Publication number
BR112015014152B1
BR112015014152B1 BR112015014152-8A BR112015014152A BR112015014152B1 BR 112015014152 B1 BR112015014152 B1 BR 112015014152B1 BR 112015014152 A BR112015014152 A BR 112015014152A BR 112015014152 B1 BR112015014152 B1 BR 112015014152B1
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BR
Brazil
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cto
nucleic acid
pto
target nucleic
acid sequence
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BR112015014152-8A
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English (en)
Portuguese (pt)
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BR112015014152A2 (enExample
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Jong Yoon Chun
Young Jo Lee
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Seegene, Inc.
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Publication of BR112015014152A2 publication Critical patent/BR112015014152A2/pt
Publication of BR112015014152B1 publication Critical patent/BR112015014152B1/pt

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6823Release of bound markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
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  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
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  • Microbiology (AREA)
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  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
BR112015014152-8A 2012-12-27 2013-12-27 Método e kit para detectar uma sequência de ácido nucleico alvo por ensaio de clivagem de pto e não hibridização dependente de extensão BR112015014152B1 (pt)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
KR10-2012-0154834 2012-12-27
KR20120154834 2012-12-27
KR10-2013-0008580 2013-01-25
KR20130008580 2013-01-25
KR10-2013-0034670 2013-03-29
KR20130034670 2013-03-29
PCT/KR2013/012312 WO2014104818A1 (en) 2012-12-27 2013-12-27 Detection of target nucleic acid sequence by pto cleavage and extension-dependent non-hybridization assay

Publications (2)

Publication Number Publication Date
BR112015014152A2 BR112015014152A2 (enExample) 2017-08-22
BR112015014152B1 true BR112015014152B1 (pt) 2023-01-24

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BR112015014152-8A BR112015014152B1 (pt) 2012-12-27 2013-12-27 Método e kit para detectar uma sequência de ácido nucleico alvo por ensaio de clivagem de pto e não hibridização dependente de extensão

Country Status (12)

Country Link
US (2) US9783845B2 (enExample)
EP (1) EP2943586B1 (enExample)
JP (1) JP6178430B2 (enExample)
KR (1) KR101760267B1 (enExample)
CN (1) CN104903465B (enExample)
AU (1) AU2013371128B2 (enExample)
BR (1) BR112015014152B1 (enExample)
CA (1) CA2896213C (enExample)
ES (1) ES2653642T3 (enExample)
MX (1) MX361191B (enExample)
RU (1) RU2620958C2 (enExample)
WO (1) WO2014104818A1 (enExample)

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KR102301305B1 (ko) 2016-06-30 2021-09-13 주식회사 씨젠 핵산 증폭용 튜브 및 핵산 증폭용 반응 용기
KR102239375B1 (ko) 2016-10-06 2021-04-13 주식회사 씨젠 시료 내 타겟 핵산분자의 검출에 이용되는 올리고뉴클레오타이드의 제공방법
WO2018114674A1 (de) * 2016-12-23 2018-06-28 Albert-Ludwigs-Universität Freiburg Zweiteilige mediatorsonde
KR102651626B1 (ko) 2016-12-29 2024-03-26 주식회사 씨젠 프라이머 다이머 형성을 감소시키고 증폭 효율을 증가시키는 방법
KR102408564B1 (ko) 2017-03-28 2022-06-14 주식회사 씨젠 타겟 핵산 서열의 존재를 결정하기 위한 분석 시그널
CN108823287B (zh) 2017-04-28 2019-04-12 厦门大学 一种检测靶核酸序列的方法
CN107236815A (zh) * 2017-07-18 2017-10-10 江西贤聚景欣医药生物科技有限公司 用于靶核酸序列检测的多重淬灭荧光探针及方法
EP3676400A4 (en) 2017-08-31 2021-06-02 Seegene, Inc. EVALUATING THE PERFORMANCE OF COMPONENTS USING A PAIR OF DIMERING PRIMERS
KR102345601B1 (ko) 2017-09-29 2021-12-30 주식회사 씨젠 Pto 절단 및 연장-의존적 연장 분석에 의한 타겟 핵산 서열의 검출
WO2019135567A1 (en) 2018-01-05 2019-07-11 Seegene, Inc. . Method for determining the presence or absence of m. tuberculosis, m. bovis and m. bovis bcg in a sample
AU2019255933B2 (en) 2018-04-20 2022-08-25 Seegene, Inc. . Method and apparatus for detecting a plurality of target nucleic acid sequences in sample
US20220025449A1 (en) 2018-12-14 2022-01-27 Seegene, Inc. Method for detecting a target analyte in a sample using an s-shaped function for a slope data set
EP3899035B1 (en) * 2018-12-20 2025-04-16 F. Hoffmann-La Roche AG Detection of target nucleic acid by solid-phase molography
WO2023043280A1 (ko) 2021-09-17 2023-03-23 주식회사 씨젠 합성 비자연 염기를 포함하는 태그 올리고뉴클레오타이드를 이용한 타겟 핵산 서열의 검출
US20250340928A1 (en) * 2022-04-22 2025-11-06 Albert-Ludwigs-Universität Freiburg Nucleic acid detection in a pcr by means of a target-sequence-unspecific modular reporter complex
EP4265734A1 (de) * 2022-04-22 2023-10-25 Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. Nukleinsäurenachweis in einer pcr mittels eines zielsequenz-unspezifischem modularer reporterkomplexes
KR20250086619A (ko) 2022-10-14 2025-06-13 주식회사 씨젠 상이한 tm 값을 가지는 2개의 프로브를 이용하여 샘플 내 타겟 핵산을 검출하는 방법
EP4372100A1 (en) * 2022-11-15 2024-05-22 Stilla Technologies Data analysis method for multiplex digital pcr using color-combination
WO2024106890A1 (ko) 2022-11-16 2024-05-23 주식회사 씨젠 퀀칭-pto 절단 및 연장(quenching-pto cleavage and extension; q-ptoce) 어세이에 의한 타겟 핵산의 검출
WO2025005717A1 (ko) * 2023-06-30 2025-01-02 주식회사 씨젠 Hto 절단 및 자기-연장 어세이에 의해 샘플 내의 타겟 핵산을 검출하는 방법

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Publication number Publication date
MX361191B (es) 2018-11-29
EP2943586A4 (en) 2016-09-28
KR20150093766A (ko) 2015-08-18
KR101760267B1 (ko) 2017-07-24
MX2015008494A (es) 2015-09-10
US20180057869A1 (en) 2018-03-01
CN104903465A (zh) 2015-09-09
US20160060690A1 (en) 2016-03-03
ES2653642T3 (es) 2018-02-08
EP2943586B1 (en) 2017-09-20
AU2013371128B2 (en) 2017-08-10
CA2896213C (en) 2018-12-11
US9783845B2 (en) 2017-10-10
RU2015130335A (ru) 2017-01-30
BR112015014152A2 (enExample) 2017-08-22
CN104903465B (zh) 2017-04-26
EP2943586A1 (en) 2015-11-18
JP2016502861A (ja) 2016-02-01
CA2896213A1 (en) 2014-07-03
JP6178430B2 (ja) 2017-08-09
RU2620958C2 (ru) 2017-05-30
AU2013371128A1 (en) 2015-08-13
WO2014104818A1 (en) 2014-07-03

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
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B06I Publication of requirement cancelled [chapter 6.9 patent gazette]

Free format text: ANULADA A PUBLICACAO CODIGO 6.6.1 NA RPI NO 2462 DE 13/03/2018 POR TER SIDO INDEVIDA.

B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B09W Correction of the decision to grant [chapter 9.1.4 patent gazette]

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B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 27/12/2013, OBSERVADAS AS CONDICOES LEGAIS