ES2653642T3 - Detección de una secuencia de ácido nucleico diana mediante ensayo sin hibridación dependiente de escisión y extensión de PTO - Google Patents
Detección de una secuencia de ácido nucleico diana mediante ensayo sin hibridación dependiente de escisión y extensión de PTO Download PDFInfo
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- ES2653642T3 ES2653642T3 ES13868457.6T ES13868457T ES2653642T3 ES 2653642 T3 ES2653642 T3 ES 2653642T3 ES 13868457 T ES13868457 T ES 13868457T ES 2653642 T3 ES2653642 T3 ES 2653642T3
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Classifications
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6823—Release of bound markers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
Landscapes
- Chemical & Material Sciences (AREA)
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- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (7)
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| KR20120154834 | 2012-12-27 | ||
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| KR20130008580 | 2013-01-25 | ||
| KR20130008580 | 2013-01-25 | ||
| KR20130034670 | 2013-03-29 | ||
| KR20130034670 | 2013-03-29 | ||
| PCT/KR2013/012312 WO2014104818A1 (en) | 2012-12-27 | 2013-12-27 | Detection of target nucleic acid sequence by pto cleavage and extension-dependent non-hybridization assay |
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| ES2653642T3 true ES2653642T3 (es) | 2018-02-08 |
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| Country | Link |
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| EP (1) | EP2943586B1 (enExample) |
| JP (1) | JP6178430B2 (enExample) |
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| MX (1) | MX361191B (enExample) |
| RU (1) | RU2620958C2 (enExample) |
| WO (1) | WO2014104818A1 (enExample) |
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| KR102264865B1 (ko) | 2016-06-30 | 2021-06-14 | 주식회사 씨젠 | 반응 분석용 형광 검출 장치 |
| KR102239375B1 (ko) | 2016-10-06 | 2021-04-13 | 주식회사 씨젠 | 시료 내 타겟 핵산분자의 검출에 이용되는 올리고뉴클레오타이드의 제공방법 |
| BR112019012925A2 (pt) * | 2016-12-23 | 2019-12-10 | Albert-Ludwigs-Universität Freiburg | sonda mediadora em duas partes |
| KR102651626B1 (ko) | 2016-12-29 | 2024-03-26 | 주식회사 씨젠 | 프라이머 다이머 형성을 감소시키고 증폭 효율을 증가시키는 방법 |
| CN110475871B (zh) | 2017-03-28 | 2024-04-12 | Seegene株式会社 | 用于确定靶核酸序列的存在的分析信号 |
| CN108823287B (zh) | 2017-04-28 | 2019-04-12 | 厦门大学 | 一种检测靶核酸序列的方法 |
| CN107236815A (zh) * | 2017-07-18 | 2017-10-10 | 江西贤聚景欣医药生物科技有限公司 | 用于靶核酸序列检测的多重淬灭荧光探针及方法 |
| US11591645B2 (en) | 2017-08-31 | 2023-02-28 | Seegene, Inc. | Evaluation of performance of components using a pair of dimer-forming primers |
| WO2019066461A2 (en) | 2017-09-29 | 2019-04-04 | Seegene, Inc. | DETECTION OF TARGET NUCLEIC ACID SEQUENCES BY CLEAVAGE ANALYSIS AND EXTENSION OF PTO |
| KR102369388B1 (ko) | 2018-01-05 | 2022-03-02 | 주식회사 씨젠 | 시료에서 M. tuberculosis, M. bovis 및 M. bovis BCG의 존재 또는 부존재를 결정하는 방법 |
| IL277870B2 (en) | 2018-04-20 | 2025-02-01 | Seegene Inc | Method and device for identifying diverse targets of nucleic acid sequences in a sample |
| US20220025449A1 (en) | 2018-12-14 | 2022-01-27 | Seegene, Inc. | Method for detecting a target analyte in a sample using an s-shaped function for a slope data set |
| EP3899035B1 (en) * | 2018-12-20 | 2025-04-16 | F. Hoffmann-La Roche AG | Detection of target nucleic acid by solid-phase molography |
| KR102894467B1 (ko) | 2021-03-05 | 2025-12-03 | 주식회사 씨젠 | SARS-CoV-2 변이를 검출하는 방법 |
| EP4403645A4 (en) * | 2021-09-17 | 2025-10-08 | Seegene Inc | DETECTION OF A TARGET NUCLEIC ACID SEQUENCE USING A MARKER OLIGONUCLEOTIDE CARRYING A SYNTHETIC NON-NATURAL BASE |
| EP4265734A1 (de) * | 2022-04-22 | 2023-10-25 | Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. | Nukleinsäurenachweis in einer pcr mittels eines zielsequenz-unspezifischem modularer reporterkomplexes |
| US20250340928A1 (en) * | 2022-04-22 | 2025-11-06 | Albert-Ludwigs-Universität Freiburg | Nucleic acid detection in a pcr by means of a target-sequence-unspecific modular reporter complex |
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| US12643104B2 (en) | 2022-10-28 | 2026-06-02 | Seegene, Inc. | Cartridge for detecting target analyte |
| EP4372100A1 (en) * | 2022-11-15 | 2024-05-22 | Stilla Technologies | Data analysis method for multiplex digital pcr using color-combination |
| EP4621068A1 (en) | 2022-11-16 | 2025-09-24 | Seegene, Inc. | Detection of target nucleic acid by quenching-pto cleavage and extension (q-ptoce) assay |
| KR20260003722A (ko) * | 2023-06-30 | 2026-01-07 | 주식회사 씨젠 | Hto 절단 및 자기-연장 어세이에 의해 샘플 내의 타겟 핵산을 검출하는 방법 |
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| GB0205455D0 (en) * | 2002-03-07 | 2002-04-24 | Molecular Sensing Plc | Nucleic acid probes, their synthesis and use |
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| WO2012150749A1 (en) * | 2011-05-04 | 2012-11-08 | Seegene, Inc. | Detection of target nucleic acid sequences by po cleavage and hybridization |
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| JP2016502861A (ja) | 2016-02-01 |
| CN104903465B (zh) | 2017-04-26 |
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| MX2015008494A (es) | 2015-09-10 |
| KR101760267B1 (ko) | 2017-07-24 |
| WO2014104818A1 (en) | 2014-07-03 |
| EP2943586B1 (en) | 2017-09-20 |
| CN104903465A (zh) | 2015-09-09 |
| CA2896213C (en) | 2018-12-11 |
| US20160060690A1 (en) | 2016-03-03 |
| AU2013371128A1 (en) | 2015-08-13 |
| AU2013371128B2 (en) | 2017-08-10 |
| US9783845B2 (en) | 2017-10-10 |
| JP6178430B2 (ja) | 2017-08-09 |
| BR112015014152B1 (pt) | 2023-01-24 |
| EP2943586A4 (en) | 2016-09-28 |
| US20180057869A1 (en) | 2018-03-01 |
| BR112015014152A2 (enExample) | 2017-08-22 |
| CA2896213A1 (en) | 2014-07-03 |
| KR20150093766A (ko) | 2015-08-18 |
| MX361191B (es) | 2018-11-29 |
| RU2015130335A (ru) | 2017-01-30 |
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