CN104583393A - 病毒样颗粒的纯化 - Google Patents

病毒样颗粒的纯化 Download PDF

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Publication number
CN104583393A
CN104583393A CN201380043618.XA CN201380043618A CN104583393A CN 104583393 A CN104583393 A CN 104583393A CN 201380043618 A CN201380043618 A CN 201380043618A CN 104583393 A CN104583393 A CN 104583393A
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vlps
solution
vlp
norovirus
chromatography
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Chinese (zh)
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R·泰勒
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Takeda Vaccines Inc
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Takeda Vaccines Inc
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Priority to CN202111173161.7A priority Critical patent/CN114085273B/zh
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Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/20—Antivirals for DNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/14011—Baculoviridae
    • C12N2710/14111—Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
    • C12N2710/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2710/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/16011—Caliciviridae
    • C12N2770/16022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/16011—Caliciviridae
    • C12N2770/16023—Virus like particles [VLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/16011—Caliciviridae
    • C12N2770/16051—Methods of production or purification of viral material

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Virology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Communicable Diseases (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Oncology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Public Health (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Analytical Chemistry (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
CN201380043618.XA 2012-06-22 2013-06-24 病毒样颗粒的纯化 Pending CN104583393A (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202111173161.7A CN114085273B (zh) 2012-06-22 2013-06-24 病毒样颗粒的纯化

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261663218P 2012-06-22 2012-06-22
US61/663,218 2012-06-22
US201361794086P 2013-03-15 2013-03-15
US61/794,086 2013-03-15
PCT/US2013/047249 WO2013192604A1 (en) 2012-06-22 2013-06-24 Purification of virus like particles

Related Child Applications (1)

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CN202111173161.7A Division CN114085273B (zh) 2012-06-22 2013-06-24 病毒样颗粒的纯化

Publications (1)

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CN104583393A true CN104583393A (zh) 2015-04-29

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CN201380043618.XA Pending CN104583393A (zh) 2012-06-22 2013-06-24 病毒样颗粒的纯化
CN202111173161.7A Active CN114085273B (zh) 2012-06-22 2013-06-24 病毒样颗粒的纯化

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Country Status (15)

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US (3) US10167320B2 (https=)
EP (1) EP2864478B1 (https=)
JP (1) JP6553509B2 (https=)
KR (1) KR102110876B1 (https=)
CN (2) CN104583393A (https=)
AR (1) AR091538A1 (https=)
AU (1) AU2013277959B2 (https=)
CA (1) CA2879815A1 (https=)
IN (1) IN2015DN00089A (https=)
JO (1) JOP20130186B1 (https=)
MX (1) MX366234B (https=)
SG (2) SG10201610753YA (https=)
TW (1) TWI647310B (https=)
UY (1) UY34875A (https=)
WO (1) WO2013192604A1 (https=)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105339495A (zh) * 2013-07-11 2016-02-17 宝生物工程株式会社 制备无包膜病毒的方法
CN105779399A (zh) * 2016-03-28 2016-07-20 广西壮族自治区林业科学研究院 一种昆虫杆状病毒纯化方法
CN105907729A (zh) * 2015-05-05 2016-08-31 广东东阳光药业有限公司 一种制备人乳头瘤病毒l1蛋白类病毒样颗粒的方法
CN107034198A (zh) * 2015-07-15 2017-08-11 长春百克生物科技股份公司 一种嵌合诺如病毒p颗粒及其制备和应用
CN108430502A (zh) * 2015-08-28 2018-08-21 科学生物技术公司 诺瓦克病毒疫苗
CN108676781A (zh) * 2018-05-18 2018-10-19 山东信得动物疫苗有限公司 一种重组禽流感病毒的工业化纯化方法
CN109689092A (zh) * 2016-09-01 2019-04-26 武田疫苗股份有限公司 用于产生用于疫苗生产的病毒的方法
CN116789773A (zh) * 2022-11-30 2023-09-22 华北制药金坦生物技术股份有限公司 一种胞内表达病毒样颗粒的粗纯方法

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG10202012986SA (en) 2006-09-29 2021-01-28 Ligocyte Pharmaceuticals Inc Norovirus vaccine formulations
DK2134360T3 (en) 2007-03-14 2016-02-22 Takeda Vaccines Inc Purification of virus-like particle
JP2010539192A (ja) 2007-09-18 2010-12-16 リゴサイト ファーマスーティカルズ，インコーポレイテッド ノロウイルスに対して防御免疫応答を付与する方法
CA2841356C (en) 2011-07-11 2022-03-01 Takeda Vaccines, Inc. Parenteral norovirus vaccine formulations
JOP20130186B1 (ar) 2012-06-22 2021-08-17 Takeda Vaccines Montana Inc تنقية الجزيئات الشبيهة بالفيروسات
KR102649950B1 (ko) 2013-10-03 2024-03-22 다케다 백신즈 인코포레이티드 세포주로부터 랍도바이러스를 탐지 및 제거하는 방법
CN103981084B (zh) * 2014-05-29 2015-11-18 黑龙江省科学院微生物研究所 水中病毒分离提取装置及水中札幌病毒的提取方法
WO2016111343A1 (ja) * 2015-01-09 2016-07-14 タカラバイオ株式会社 非エンベロープウイルス粒子の製造方法
EP3245306A4 (en) 2015-01-16 2018-09-05 Takeda Vaccines, Inc. Detection of particle-contained reverse transcriptase activity
GB201614050D0 (en) * 2016-08-17 2016-09-28 Glaxosmithkline Ip Dev Ltd Method for purifying viral vectors
EP4382183A3 (en) 2017-03-06 2024-08-21 Guangzhou Realbenefitspot Pharmaceutical Co., Ltd. Methods of producing and characterizing virus vaccine and virus vaccine composition
EP3420076B1 (en) 2017-03-06 2024-02-21 Guangzhou Realbenefitspot Pharmaceutical Co., Ltd. Methods of producing and characterizing virus vaccine and virus vaccine composition
US11633467B2 (en) 2018-02-15 2023-04-25 Icon Genetics Gmbh Immunogenic composition and vaccine for generating an immune response to norovirus
CN108776225A (zh) * 2018-05-23 2018-11-09 中国农业科学院兰州兽医研究所 猪细小病毒VLPs抗体检测试剂盒及其制备方法、应用
AU2020235344B2 (en) 2019-03-12 2024-10-24 Icon Genetics Gmbh Norovirus-like particles with improved stability
JP7803014B2 (ja) 2019-09-30 2026-01-21 バイオベラティブ セラピューティクス インコーポレイテッド レンチウイルスベクター製剤
US20240238401A1 (en) 2021-05-21 2024-07-18 Takeda Vaccines, Inc. Solid composition, freeze-drying method and glass vial
US12371455B2 (en) * 2021-06-28 2025-07-29 Vst Llc Sapovirus vaccines
WO2025077800A1 (en) * 2023-10-12 2025-04-17 Chengdu Kanghua Biological Products Co., Ltd. Purification of norovirus virus-like particles (vlps)
CN118126202B (zh) * 2024-05-07 2024-07-30 上海瑞宙生物科技有限公司 一种诺如病毒类病毒颗粒及其应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100150961A1 (en) * 2007-03-14 2010-06-17 Vedvick Thomas S Virus like particle purification
US20120156243A1 (en) * 2006-09-29 2012-06-21 LigoCyte Pharmaceutical Norovirus vaccine formulations

Family Cites Families (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2833106B2 (ja) 1990-02-21 1998-12-09 株式会社島津製作所 液体クロマトグラフ
WO1994026087A2 (en) 1993-05-14 1994-11-24 Connor Kim C O Recombinant protein production and insect cell culture and process
JP3863559B2 (ja) 1994-05-16 2006-12-27 メルク エンド カンパニー インコーポレーテッド 乳頭腫ウィルスワクチン
AR004464A1 (es) 1994-11-14 1998-12-16 Merck Sharp & Dohme Un metodo para producir una proteina de capside de papilomavirus
JP3316325B2 (ja) * 1994-12-20 2002-08-19 富士シリシア化学株式会社 ビール安定化処理用シリカゲル及びその製造方法並びにビールの安定化処理方法
US5641870A (en) 1995-04-20 1997-06-24 Genentech, Inc. Low pH hydrophobic interaction chromatography for antibody purification
CA2269097C (en) 1996-11-08 2007-01-09 The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services Synthesis and purification of hepatitis c virus-like particles
ES2572628T3 (es) 1997-09-05 2016-06-01 Medimmune, Inc. Método in vitro para desmontaje/remontaje de partículas análogas al virus del papiloma humano (VLP)
DE19823814A1 (de) 1998-05-27 1999-12-02 Octapharma Ag Ziegelbruecke Verfahren zur Trennung und/oder Isolierung von Plasmaproteinen mit annularer Chromatographie
AU760633B2 (en) 1998-08-14 2003-05-22 Merck Sharp & Dohme Corp. Process for purifying human papillomavirus virus-like particles
DE60040727D1 (de) * 1999-02-05 2008-12-18 Merck & Co Inc Menschliche papillomavirus impfstoff-formulierungen
US6349843B1 (en) 1999-04-01 2002-02-26 The Vollrath Company Llc Pan removal ramp
ES2398413T3 (es) 1999-06-22 2013-03-19 Japan As Represented By Director-General Of National Institute Of Infectious Diseases Kit de detección de SRSV
DE10012732A1 (de) 2000-03-18 2001-09-20 Aventis Behring Gmbh Thrombin-Zubereitungen und Verfahren zu ihrer Herstellung
JP2003066021A (ja) 2001-08-28 2003-03-05 Hiroshi Shionoya 連続分取液体クロマトグラフィー装置とそれを用いる方法
WO2003068163A2 (en) 2002-02-14 2003-08-21 Novavax, Inc. Optimization of gene sequences of chimeric virus-like particles for expression in insect cells
CA2478925C (en) 2002-04-26 2016-06-07 Robert Lee Fahrner Non-affinity purification of proteins
CA2496918A1 (en) 2002-08-28 2004-03-11 Introgen Therapeutics Inc. Chromatographic methods for adenovirus purification
WO2005032457A2 (en) 2003-07-21 2005-04-14 Boyce Thompson Institute For Plant Research, Inc. Vectors and methods for immunization against norwalk virus using transgenic plants
US7481997B1 (en) 2004-02-12 2009-01-27 Montana State University Snow mountain virus genome sequence, virus-like particles and methods of use
WO2006138514A2 (en) 2005-06-16 2006-12-28 Children's Hospital Medical Center Novel norovirus particle for use as an antiviral or vaccine
EP1736538A1 (en) 2005-06-21 2006-12-27 Cytos Biotechnology AG Process for the preparative purification of virus-like-particles (VLPs)
CA2630220C (en) 2005-11-22 2020-10-13 Doris Coit Norovirus and sapovirus antigens
EP3868400A1 (en) 2005-12-29 2021-08-25 Boehringer Ingelheim Animal Health USA Inc. Multivalent pcv2 immunogenic compositions and methods of producing such compositions
US9439959B2 (en) 2006-07-27 2016-09-13 Takeda Vaccines, Inc. Chimeric influenza virus-like particles
US8202967B2 (en) 2006-10-27 2012-06-19 Boehringer Ingelheim Vetmedica, Inc. H5 proteins, nucleic acid molecules and vectors encoding for those, and their medicinal use
EP2027875A1 (en) 2007-08-23 2009-02-25 Octapharma AG A Process for Isolation and Purification of a Target Protein free of Prion Protein (PrPSC)
JP2010539192A (ja) 2007-09-18 2010-12-16 リゴサイト ファーマスーティカルズ，インコーポレイテッド ノロウイルスに対して防御免疫応答を付与する方法
EP2235165A1 (en) 2007-12-21 2010-10-06 Cytos Biotechnology AG Process for clarifying cell homogenates
AU2009279456B2 (en) 2008-08-08 2015-02-05 Takeda Vaccines, Inc. Virus-like particles comprising composite capsid amino acid sequences for enhanced cross reactivity
AU2009319979B2 (en) 2008-11-03 2016-10-20 Takeda Vaccines, Inc. Improved methods for isolating enveloped virus-based VLPs free of infectious agents
US8945895B2 (en) * 2009-07-31 2015-02-03 Baxter International Inc. Methods of purifying recombinant ADAMTS13 and other proteins and compositions thereof
KR20120125627A (ko) 2010-01-21 2012-11-16 리고사이트 파머슈티컬즈 인코퍼레이티드 칼리시바이러스 바이러스 유사 입자들 상에 표적화된 이종 항원 제공
JP5903101B2 (ja) 2010-09-20 2016-04-13 バイオ−ラッド ラボラトリーズ インコーポレーティッド クロマトグラフィーにおける生成物と複合体形成した夾雑物の分離方法
FI122520B (fi) 2010-10-15 2012-03-15 Vesna Blazevic Noroviruksen kapsidi ja rotaviruksen VP6-proteiini käytettäväksi yhdistelmärokotteena
CN102260651A (zh) 2011-06-24 2011-11-30 华中科技大学同济医学院附属协和医院 一种纯化病毒样颗粒蛋白的方法及其应用
JOP20130186B1 (ar) 2012-06-22 2021-08-17 Takeda Vaccines Montana Inc تنقية الجزيئات الشبيهة بالفيروسات
JP2015015931A (ja) 2013-07-12 2015-01-29 株式会社Ｕｍｎファーマ ウイルス様粒子を含む培養物の製造方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120156243A1 (en) * 2006-09-29 2012-06-21 LigoCyte Pharmaceutical Norovirus vaccine formulations
US20100150961A1 (en) * 2007-03-14 2010-06-17 Vedvick Thomas S Virus like particle purification

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
GABRIEL I. PARRA，ET AL: "Immunogenicity and specificity of norovirus Consensus GII.4 virus-like particles in monovalent and bivalent vaccine formulations", 《VACCINE》 *
HUAFANG LAI • QIANG CHEN: "Bioprocessing of plant-derived virus-like particlesof Norwalk virus capsid protein under current Good Manufacture Practice regulations", 《PLANT CELL REP》 *
L. HUHTI，ET AL: "A comparison of methods for purification and concentration of norovirus GII-4 capsid virus-like particles", 《ARCH VIROL》 *

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105339495A (zh) * 2013-07-11 2016-02-17 宝生物工程株式会社 制备无包膜病毒的方法
CN105907729B (zh) * 2015-05-05 2019-08-16 广东东阳光药业有限公司 一种制备人乳头瘤病毒l1蛋白类病毒样颗粒的方法
CN105907729A (zh) * 2015-05-05 2016-08-31 广东东阳光药业有限公司 一种制备人乳头瘤病毒l1蛋白类病毒样颗粒的方法
US11116831B2 (en) 2015-07-15 2021-09-14 Changchun Bcht Biotechnology Co. Chimeric norovirus P particle and preparation and use thereof
CN107034198A (zh) * 2015-07-15 2017-08-11 长春百克生物科技股份公司 一种嵌合诺如病毒p颗粒及其制备和应用
CN107034198B (zh) * 2015-07-15 2021-03-09 长春百克生物科技股份公司 一种嵌合诺如病毒p颗粒及其制备和应用
CN108430502A (zh) * 2015-08-28 2018-08-21 科学生物技术公司 诺瓦克病毒疫苗
US11596680B2 (en) 2015-08-28 2023-03-07 Resilience Government Services, Inc. Norovirus vaccine
CN105779399A (zh) * 2016-03-28 2016-07-20 广西壮族自治区林业科学研究院 一种昆虫杆状病毒纯化方法
CN109689092A (zh) * 2016-09-01 2019-04-26 武田疫苗股份有限公司 用于产生用于疫苗生产的病毒的方法
CN108676781A (zh) * 2018-05-18 2018-10-19 山东信得动物疫苗有限公司 一种重组禽流感病毒的工业化纯化方法
CN116789773A (zh) * 2022-11-30 2023-09-22 华北制药金坦生物技术股份有限公司 一种胞内表达病毒样颗粒的粗纯方法
CN116789773B (zh) * 2022-11-30 2024-02-20 华北制药金坦生物技术股份有限公司 一种胞内表达病毒样颗粒的粗纯方法

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US20140004145A1 (en) 2014-01-02
EP2864478B1 (en) 2020-12-09
US11091519B2 (en) 2021-08-17
TW201406956A (zh) 2014-02-16
CN114085273B (zh) 2024-07-12
MX366234B (es) 2019-07-03
JP6553509B2 (ja) 2019-07-31
SG11201408459VA (en) 2015-01-29
WO2013192604A1 (en) 2013-12-27
JP2015521628A (ja) 2015-07-30
IN2015DN00089A (https=) 2015-05-29
EP2864478A1 (en) 2015-04-29
AU2013277959A1 (en) 2015-01-22
EP2864478A4 (en) 2015-12-02
CN114085273A (zh) 2022-02-25
KR20150051988A (ko) 2015-05-13
UY34875A (es) 2013-12-31
US20190169236A1 (en) 2019-06-06
US12286456B2 (en) 2025-04-29
SG10201610753YA (en) 2017-02-27
KR102110876B1 (ko) 2020-05-14
CA2879815A1 (en) 2013-12-27
TWI647310B (zh) 2019-01-11
HK1210210A1 (en) 2016-04-15
JOP20130186B1 (ar) 2021-08-17
MX2014015842A (es) 2015-09-07
US10167320B2 (en) 2019-01-01
AU2013277959B2 (en) 2019-05-16
AR091538A1 (es) 2015-02-11
US20220213147A1 (en) 2022-07-07

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