CN104245958B - 核酸的检测 - Google Patents
核酸的检测 Download PDFInfo
- Publication number
- CN104245958B CN104245958B CN201380019973.3A CN201380019973A CN104245958B CN 104245958 B CN104245958 B CN 104245958B CN 201380019973 A CN201380019973 A CN 201380019973A CN 104245958 B CN104245958 B CN 104245958B
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- nucleotides
- polynucleotide
- oligonucleotide
- sequence
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2012900624A AU2012900624A0 (en) | 2012-02-20 | Detection of Nucleic Acids | |
| AU2012900624 | 2012-02-20 | ||
| AU2012902218A AU2012902218A0 (en) | 2012-05-29 | Detection of nucleic acids | |
| AU2012902218 | 2012-05-29 | ||
| PCT/AU2013/000149 WO2013123552A1 (en) | 2012-02-20 | 2013-02-20 | Detection of nucleic acids |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104245958A CN104245958A (zh) | 2014-12-24 |
| CN104245958B true CN104245958B (zh) | 2018-05-22 |
Family
ID=49004851
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201380019973.3A Active CN104245958B (zh) | 2012-02-20 | 2013-02-20 | 核酸的检测 |
Country Status (17)
| Country | Link |
|---|---|
| US (3) | US9963738B2 (https=) |
| EP (1) | EP2817421B1 (https=) |
| JP (2) | JP6276201B2 (https=) |
| KR (1) | KR102085119B1 (https=) |
| CN (1) | CN104245958B (https=) |
| AU (1) | AU2013202920B9 (https=) |
| BR (1) | BR112014020422B1 (https=) |
| CA (1) | CA2863808C (https=) |
| DK (1) | DK2817421T3 (https=) |
| ES (1) | ES2660248T3 (https=) |
| IL (1) | IL233958A (https=) |
| MX (1) | MX353840B (https=) |
| NO (1) | NO2817421T3 (https=) |
| NZ (1) | NZ628883A (https=) |
| SG (1) | SG11201405012YA (https=) |
| WO (1) | WO2013123552A1 (https=) |
| ZA (1) | ZA201406410B (https=) |
Families Citing this family (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103917664B (zh) * | 2011-09-09 | 2018-06-15 | 斯比戴克斯私人有限公司 | 核酸酶底物 |
| CA2863808C (en) | 2012-02-20 | 2020-07-21 | Speedx Pty Ltd | Methods and oligonucleotides for amplifying and/or detecting nucleic acids |
| AU2013202354A1 (en) * | 2012-06-18 | 2014-01-16 | Speedx Pty Ltd | Target detection and signal amplification |
| KR102351434B1 (ko) | 2013-02-07 | 2022-01-17 | 러트거즈,더스테이트유니버시티오브뉴저지 | 고도로 선택적인 핵산 증폭 프라이머 |
| CN103540660B (zh) * | 2013-10-10 | 2015-10-28 | 中国人民解放军疾病预防控制所 | 基于TaqMan探针的环介导恒温扩增法及其专用LAMP引物与试剂盒 |
| CN106460044B (zh) * | 2014-04-25 | 2021-03-19 | DNAe集团控股有限公司 | 测序方法 |
| KR101631114B1 (ko) * | 2014-06-13 | 2016-06-16 | 서울대학교산학협력단 | 육종감별을 위한 등온증폭반응용 프라이머 세트 및 이를 이용한 육종 감별 방법 |
| US20170335382A1 (en) * | 2014-11-10 | 2017-11-23 | University Of Pittsburgh - Of The Commonwealth System Of Higher Education | Isothermal Amplification Assay for the Detection of Short Nucleic Acid Sequences |
| CN107614697A (zh) * | 2015-02-26 | 2018-01-19 | 奥斯瑞根公司 | 用于提高突变评估准确性的方法和装置 |
| CN104845967B (zh) * | 2015-04-15 | 2020-12-11 | 苏州新海生物科技股份有限公司 | 寡聚核苷酸片段及使用其的选择性扩增目标核酸序列变异体的方法及应用 |
| CN108026569B (zh) * | 2015-07-17 | 2021-08-24 | 卢米耐克斯公司 | 用于催化性测定的方法和组合物 |
| US20200299754A1 (en) | 2017-04-11 | 2020-09-24 | Speedx Pty Ltd | Detection cascades |
| EP3665305A1 (en) | 2017-08-10 | 2020-06-17 | Katholieke Universiteit Leuven | Nucleic acid enzyme sensor |
| GB201713251D0 (en) * | 2017-08-18 | 2017-10-04 | Lgc Genomics Ltd | Methods and kits for detection of nucleic acid molecules |
| KR101987388B1 (ko) * | 2017-10-19 | 2019-06-12 | 경상대학교산학협력단 | 분자비콘을 포함하는 비브리오균의 등온비색 검출용 조성물 및 이의 용도 |
| CN107966438B (zh) * | 2017-10-27 | 2020-11-24 | 中国农业大学 | 一种基于锌的功能核酸的耐高盐传感器及其应用 |
| WO2019150414A1 (ja) * | 2018-01-30 | 2019-08-08 | 学校法人 慶應義塾 | 核酸の検出方法 |
| IL280772B2 (en) * | 2018-08-09 | 2025-04-01 | Speedx Pty Ltd | Multiplex nucleic acid testing |
| CN109097468A (zh) * | 2018-08-14 | 2018-12-28 | 南通市第人民医院 | Opcml基因启动子甲基化水平的检测试剂盒及其应用 |
| CN110452988A (zh) * | 2019-08-27 | 2019-11-15 | 武汉友芝友医疗科技股份有限公司 | 检测esr1基因突变的引物组、试剂、试剂盒和方法 |
| IL291819A (en) | 2019-10-02 | 2022-06-01 | Univ Rutgers | Assay methods and kits for detecting rare sequence variants |
| MX2022014857A (es) * | 2020-06-30 | 2023-02-23 | Speedx Pty Ltd | Deteccion multiplex de acidos nucleicos mediante el uso de indicadores mixtos. |
| KR20230098259A (ko) | 2020-10-29 | 2023-07-03 | 비오까르띠 엔브이 | 멀티플렉스 핵산 검출을 위한 제네릭 카트리지 및 방법 |
| CN112662804B (zh) * | 2021-01-25 | 2022-05-10 | 中国水稻研究所 | 一种检测稻瘟病菌无毒基因AvrPi9致病性变异的引物组、试剂盒及方法 |
| AU2022216246A1 (en) * | 2021-02-02 | 2023-09-14 | Rhodx, Inc. | Synthetic polynucleotides and methods for selectively amplifying alleles |
| CN112852934B (zh) * | 2021-04-16 | 2022-02-18 | 武汉友芝友医疗科技股份有限公司 | 检测基因甲基化的引物、试剂和试剂盒 |
| WO2023067110A1 (en) * | 2021-10-20 | 2023-04-27 | Tataa Biocenter Ab | Methods and compositions for detection of mutant nucleic acid sequences |
| CN114609105B (zh) * | 2022-03-11 | 2024-12-13 | 济南大学 | 一种联合检测miRNA155和谷胱甘肽的荧光生物传感器 |
| WO2025023659A1 (ko) | 2023-07-25 | 2025-01-30 | 주식회사 씨젠 | 타겟 핵산 서열에 대한 올리고뉴클레오타이드 서열의 얼라인먼트 정보를 제공하는 컴퓨터-구현 방법 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040121374A1 (en) * | 2002-10-02 | 2004-06-24 | Yoshihide Iwaki | Method for detecting single nucleotide polymorphisms |
| WO2006095941A1 (en) * | 2005-03-05 | 2006-09-14 | Seegene, Inc. | Processes using dual specificity oligonucleotide and dual specificity oligonucleotide |
| JP5665272B2 (ja) * | 2005-10-07 | 2015-02-04 | スピーデクス ピーティーワイ リミテッド | 多成分核酸酵素およびそれらの使用方法 |
| ES2520023T3 (es) | 2006-10-06 | 2014-11-11 | Speedx Pty Ltd | Interruptores moleculares y métodos para su uso |
| US8962238B2 (en) * | 2007-04-05 | 2015-02-24 | SpeedDx Pty Ltd | Nucleic acid enzymes and complexes and methods for their use |
| JP2010035532A (ja) * | 2008-08-08 | 2010-02-18 | Canon Inc | アレル特異的ｐｃｒ法 |
| CN102459630B (zh) | 2009-06-29 | 2014-04-30 | 株式会社东芝 | 样本分析方法和用于该方法的检测试剂盒 |
| JP5115825B2 (ja) * | 2009-11-06 | 2013-01-09 | 独立行政法人農業・食品産業技術総合研究機構 | イグサ品種「ひのみどり」の識別マーカー |
| JP5822843B2 (ja) * | 2009-12-21 | 2015-11-24 | シージーン アイエヌシー | Ｔｓｇプライマーターゲット検出 |
| JP5684737B2 (ja) * | 2010-01-21 | 2015-03-18 | アークレイ株式会社 | 標的配列の増幅方法、多型検出方法およびそれに用いる試薬 |
| CA2863808C (en) * | 2012-02-20 | 2020-07-21 | Speedx Pty Ltd | Methods and oligonucleotides for amplifying and/or detecting nucleic acids |
-
2013
- 2013-02-20 CA CA2863808A patent/CA2863808C/en active Active
- 2013-02-20 SG SG11201405012YA patent/SG11201405012YA/en unknown
- 2013-02-20 ES ES13751988.0T patent/ES2660248T3/es active Active
- 2013-02-20 NZ NZ628883A patent/NZ628883A/en unknown
- 2013-02-20 JP JP2014556886A patent/JP6276201B2/ja active Active
- 2013-02-20 NO NO13751988A patent/NO2817421T3/no unknown
- 2013-02-20 WO PCT/AU2013/000149 patent/WO2013123552A1/en not_active Ceased
- 2013-02-20 DK DK13751988.0T patent/DK2817421T3/en active
- 2013-02-20 BR BR112014020422-5A patent/BR112014020422B1/pt not_active IP Right Cessation
- 2013-02-20 CN CN201380019973.3A patent/CN104245958B/zh active Active
- 2013-02-20 US US14/379,178 patent/US9963738B2/en active Active
- 2013-02-20 KR KR1020147025284A patent/KR102085119B1/ko active Active
- 2013-02-20 AU AU2013202920A patent/AU2013202920B9/en active Active
- 2013-02-20 MX MX2014010007A patent/MX353840B/es active IP Right Grant
- 2013-02-20 EP EP13751988.0A patent/EP2817421B1/en active Active
-
2014
- 2014-08-05 IL IL233958A patent/IL233958A/en active IP Right Grant
- 2014-09-01 ZA ZA2014/06410A patent/ZA201406410B/en unknown
-
2018
- 2018-01-11 JP JP2018002618A patent/JP6836302B2/ja active Active
- 2018-03-27 US US15/937,657 patent/US10724080B2/en active Active
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2020
- 2020-06-25 US US16/912,622 patent/US11802308B2/en active Active
Non-Patent Citations (3)
| Title |
|---|
| "An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol.";Liu H.;《BMC Biotechnol.》;20081204;第8卷(第91期);1-10 * |
| "Development of multisample detection system using a tag insertion primer and an electrochemical DNA chip.";Nakamura N.;《Anal Biochem.》;20111215;第419卷(第2期);190-5 * |
| "Simple and efficient site-directed mutagenesis using two single-primer reactions in parallel to generate mutants for protein structure-function studies.";Edelheit O.;《BMC Biotechnol.》;20090630;第9卷(第61期);1-8 * |
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