KR102085119B1 - 핵산의 검출 - Google Patents

핵산의 검출 Download PDF

Info

Publication number
KR102085119B1
KR102085119B1 KR1020147025284A KR20147025284A KR102085119B1 KR 102085119 B1 KR102085119 B1 KR 102085119B1 KR 1020147025284 A KR1020147025284 A KR 1020147025284A KR 20147025284 A KR20147025284 A KR 20147025284A KR 102085119 B1 KR102085119 B1 KR 102085119B1
Authority
KR
South Korea
Prior art keywords
component
primer
delete delete
amplicon
nucleotides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
KR1020147025284A
Other languages
English (en)
Korean (ko)
Other versions
KR20140139497A (ko
Inventor
앨리슨 벨이안 토드
엘리사 모카니
사만다 월커
코어트니 도널드
디나 로너건
탠 리트 인
Original Assignee
스피디엑스 피티와이 리미티드
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2012900624A external-priority patent/AU2012900624A0/en
Application filed by 스피디엑스 피티와이 리미티드 filed Critical 스피디엑스 피티와이 리미티드
Publication of KR20140139497A publication Critical patent/KR20140139497A/ko
Application granted granted Critical
Publication of KR102085119B1 publication Critical patent/KR102085119B1/ko
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
KR1020147025284A 2012-02-20 2013-02-20 핵산의 검출 Active KR102085119B1 (ko)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
AU2012900624A AU2012900624A0 (en) 2012-02-20 Detection of Nucleic Acids
AU2012900624 2012-02-20
AU2012902218A AU2012902218A0 (en) 2012-05-29 Detection of nucleic acids
AU2012902218 2012-05-29
PCT/AU2013/000149 WO2013123552A1 (en) 2012-02-20 2013-02-20 Detection of nucleic acids

Publications (2)

Publication Number Publication Date
KR20140139497A KR20140139497A (ko) 2014-12-05
KR102085119B1 true KR102085119B1 (ko) 2020-03-05

Family

ID=49004851

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020147025284A Active KR102085119B1 (ko) 2012-02-20 2013-02-20 핵산의 검출

Country Status (17)

Country Link
US (3) US9963738B2 (https=)
EP (1) EP2817421B1 (https=)
JP (2) JP6276201B2 (https=)
KR (1) KR102085119B1 (https=)
CN (1) CN104245958B (https=)
AU (1) AU2013202920B9 (https=)
BR (1) BR112014020422B1 (https=)
CA (1) CA2863808C (https=)
DK (1) DK2817421T3 (https=)
ES (1) ES2660248T3 (https=)
IL (1) IL233958A (https=)
MX (1) MX353840B (https=)
NO (1) NO2817421T3 (https=)
NZ (1) NZ628883A (https=)
SG (1) SG11201405012YA (https=)
WO (1) WO2013123552A1 (https=)
ZA (1) ZA201406410B (https=)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103917664B (zh) * 2011-09-09 2018-06-15 斯比戴克斯私人有限公司 核酸酶底物
CA2863808C (en) 2012-02-20 2020-07-21 Speedx Pty Ltd Methods and oligonucleotides for amplifying and/or detecting nucleic acids
AU2013202354A1 (en) * 2012-06-18 2014-01-16 Speedx Pty Ltd Target detection and signal amplification
KR102351434B1 (ko) 2013-02-07 2022-01-17 러트거즈,더스테이트유니버시티오브뉴저지 고도로 선택적인 핵산 증폭 프라이머
CN103540660B (zh) * 2013-10-10 2015-10-28 中国人民解放军疾病预防控制所 基于TaqMan探针的环介导恒温扩增法及其专用LAMP引物与试剂盒
CN106460044B (zh) * 2014-04-25 2021-03-19 DNAe集团控股有限公司 测序方法
KR101631114B1 (ko) * 2014-06-13 2016-06-16 서울대학교산학협력단 육종감별을 위한 등온증폭반응용 프라이머 세트 및 이를 이용한 육종 감별 방법
US20170335382A1 (en) * 2014-11-10 2017-11-23 University Of Pittsburgh - Of The Commonwealth System Of Higher Education Isothermal Amplification Assay for the Detection of Short Nucleic Acid Sequences
CN107614697A (zh) * 2015-02-26 2018-01-19 奥斯瑞根公司 用于提高突变评估准确性的方法和装置
CN104845967B (zh) * 2015-04-15 2020-12-11 苏州新海生物科技股份有限公司 寡聚核苷酸片段及使用其的选择性扩增目标核酸序列变异体的方法及应用
CN108026569B (zh) * 2015-07-17 2021-08-24 卢米耐克斯公司 用于催化性测定的方法和组合物
US20200299754A1 (en) 2017-04-11 2020-09-24 Speedx Pty Ltd Detection cascades
EP3665305A1 (en) 2017-08-10 2020-06-17 Katholieke Universiteit Leuven Nucleic acid enzyme sensor
GB201713251D0 (en) * 2017-08-18 2017-10-04 Lgc Genomics Ltd Methods and kits for detection of nucleic acid molecules
KR101987388B1 (ko) * 2017-10-19 2019-06-12 경상대학교산학협력단 분자비콘을 포함하는 비브리오균의 등온비색 검출용 조성물 및 이의 용도
CN107966438B (zh) * 2017-10-27 2020-11-24 中国农业大学 一种基于锌的功能核酸的耐高盐传感器及其应用
WO2019150414A1 (ja) * 2018-01-30 2019-08-08 学校法人 慶應義塾 核酸の検出方法
IL280772B2 (en) * 2018-08-09 2025-04-01 Speedx Pty Ltd Multiplex nucleic acid testing
CN109097468A (zh) * 2018-08-14 2018-12-28 南通市第人民医院 Opcml基因启动子甲基化水平的检测试剂盒及其应用
CN110452988A (zh) * 2019-08-27 2019-11-15 武汉友芝友医疗科技股份有限公司 检测esr1基因突变的引物组、试剂、试剂盒和方法
IL291819A (en) 2019-10-02 2022-06-01 Univ Rutgers Assay methods and kits for detecting rare sequence variants
MX2022014857A (es) * 2020-06-30 2023-02-23 Speedx Pty Ltd Deteccion multiplex de acidos nucleicos mediante el uso de indicadores mixtos.
KR20230098259A (ko) 2020-10-29 2023-07-03 비오까르띠 엔브이 멀티플렉스 핵산 검출을 위한 제네릭 카트리지 및 방법
CN112662804B (zh) * 2021-01-25 2022-05-10 中国水稻研究所 一种检测稻瘟病菌无毒基因AvrPi9致病性变异的引物组、试剂盒及方法
AU2022216246A1 (en) * 2021-02-02 2023-09-14 Rhodx, Inc. Synthetic polynucleotides and methods for selectively amplifying alleles
CN112852934B (zh) * 2021-04-16 2022-02-18 武汉友芝友医疗科技股份有限公司 检测基因甲基化的引物、试剂和试剂盒
WO2023067110A1 (en) * 2021-10-20 2023-04-27 Tataa Biocenter Ab Methods and compositions for detection of mutant nucleic acid sequences
CN114609105B (zh) * 2022-03-11 2024-12-13 济南大学 一种联合检测miRNA155和谷胱甘肽的荧光生物传感器
WO2025023659A1 (ko) 2023-07-25 2025-01-30 주식회사 씨젠 타겟 핵산 서열에 대한 올리고뉴클레오타이드 서열의 얼라인먼트 정보를 제공하는 컴퓨터-구현 방법

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008122084A1 (en) 2007-04-05 2008-10-16 Johnson & Johnson Research Pty Limited Nucleic acid enzymes and complexes and methods for their use
WO2011001496A1 (ja) 2009-06-29 2011-01-06 株式会社 東芝 検体解析方法およびそれに用いるアッセイキット

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040121374A1 (en) * 2002-10-02 2004-06-24 Yoshihide Iwaki Method for detecting single nucleotide polymorphisms
WO2006095941A1 (en) * 2005-03-05 2006-09-14 Seegene, Inc. Processes using dual specificity oligonucleotide and dual specificity oligonucleotide
JP5665272B2 (ja) * 2005-10-07 2015-02-04 スピーデクス ピーティーワイ リミテッド 多成分核酸酵素およびそれらの使用方法
ES2520023T3 (es) 2006-10-06 2014-11-11 Speedx Pty Ltd Interruptores moleculares y métodos para su uso
JP2010035532A (ja) * 2008-08-08 2010-02-18 Canon Inc アレル特異的ｐｃｒ法
JP5115825B2 (ja) * 2009-11-06 2013-01-09 独立行政法人農業・食品産業技術総合研究機構 イグサ品種「ひのみどり」の識別マーカー
JP5822843B2 (ja) * 2009-12-21 2015-11-24 シージーン アイエヌシー Ｔｓｇプライマーターゲット検出
JP5684737B2 (ja) * 2010-01-21 2015-03-18 アークレイ株式会社 標的配列の増幅方法、多型検出方法およびそれに用いる試薬
CA2863808C (en) * 2012-02-20 2020-07-21 Speedx Pty Ltd Methods and oligonucleotides for amplifying and/or detecting nucleic acids

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008122084A1 (en) 2007-04-05 2008-10-16 Johnson & Johnson Research Pty Limited Nucleic acid enzymes and complexes and methods for their use
WO2011001496A1 (ja) 2009-06-29 2011-01-06 株式会社 東芝 検体解析方法およびそれに用いるアッセイキット

Also Published As

Publication number Publication date
ZA201406410B (en) 2015-09-30
WO2013123552A9 (en) 2014-08-28
US11802308B2 (en) 2023-10-31
HK1199743A1 (en) 2015-07-17
US20190024160A1 (en) 2019-01-24
JP6836302B2 (ja) 2021-02-24
AU2013202920A1 (en) 2013-09-05
JP6276201B2 (ja) 2018-02-07
US20160348161A1 (en) 2016-12-01
AU2013202920B2 (en) 2015-09-17
JP2018075033A (ja) 2018-05-17
SG11201405012YA (en) 2014-09-26
EP2817421A4 (en) 2016-01-06
MX353840B (es) 2018-01-31
EP2817421B1 (en) 2017-12-13
KR20140139497A (ko) 2014-12-05
AU2013202920A9 (en) 2015-08-20
ES2660248T3 (es) 2018-03-21
NO2817421T3 (https=) 2018-05-12
AU2013202920B9 (en) 2015-10-01
EP2817421A1 (en) 2014-12-31
MX2014010007A (es) 2014-09-12
DK2817421T3 (en) 2018-03-05
US20210108261A1 (en) 2021-04-15
BR112014020422A2 (pt) 2018-10-30
IL233958A (en) 2017-03-30
IL233958A0 (en) 2014-09-30
WO2013123552A1 (en) 2013-08-29
CN104245958B (zh) 2018-05-22
JP2015511818A (ja) 2015-04-23
NZ628883A (en) 2015-07-31
BR112014020422B1 (pt) 2021-12-28
CN104245958A (zh) 2014-12-24
US9963738B2 (en) 2018-05-08
US10724080B2 (en) 2020-07-28
CA2863808A1 (en) 2013-08-29
CA2863808C (en) 2020-07-21

Similar Documents

Publication Publication Date Title
KR102085119B1 (ko) 핵산의 검출
JP7175326B2 (ja) 標的核酸増幅方法及び標的核酸増幅用組成物
US10273526B2 (en) Nucleic acid enzyme substrates
BRPI0616466B1 (pt) composição de enzimas de ácido nucleico multicomponentes (mnazima), métodos para detectar a presença de pelo menos um facilitador de combinação, um alvo e uma variante de sequência de ácidos nucléicos, métodos para fabricar uma pluraridade de mnazima, testar sequências de núcleo catalítico parcial e identificar suas posições, bem como uso de pelo menos um oligonucleotídeo
JP7423603B2 (ja) 核酸の多重検出
US9051606B2 (en) Methods and compositions for nucleic acid detection
EP4310195A1 (en) Method for nucleic acid detection using signal-mediated amplification of rna technology and rna aptamers
HK1199743B (en) Detection of nucleic acids
KR20260034624A (ko) 온도 제어 프로브를 이용한 표적 검출

Legal Events

Date Code Title Description
E13-X000 Pre-grant limitation requested

St.27 status event code: A-2-3-E10-E13-lim-X000

PA0105 International application

St.27 status event code: A-0-1-A10-A15-nap-PA0105

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

PG1501 Laying open of application

St.27 status event code: A-1-1-Q10-Q12-nap-PG1501

R17-X000 Change to representative recorded

St.27 status event code: A-3-3-R10-R17-oth-X000

A201 Request for examination
E13-X000 Pre-grant limitation requested

St.27 status event code: A-2-3-E10-E13-lim-X000

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

PA0201 Request for examination

St.27 status event code: A-1-2-D10-D11-exm-PA0201

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

St.27 status event code: A-1-2-D10-D21-exm-PE0902

T11-X000 Administrative time limit extension requested

St.27 status event code: U-3-3-T10-T11-oth-X000

T11-X000 Administrative time limit extension requested

St.27 status event code: U-3-3-T10-T11-oth-X000

T11-X000 Administrative time limit extension requested

St.27 status event code: U-3-3-T10-T11-oth-X000

T11-X000 Administrative time limit extension requested

St.27 status event code: U-3-3-T10-T11-oth-X000

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

E701 Decision to grant or registration of patent right
PE0701 Decision of registration

St.27 status event code: A-1-2-D10-D22-exm-PE0701

PR0701 Registration of establishment

St.27 status event code: A-2-4-F10-F11-exm-PR0701

PR1002 Payment of registration fee

St.27 status event code: A-2-2-U10-U12-oth-PR1002

Fee payment year number: 1

PG1601 Publication of registration

St.27 status event code: A-4-4-Q10-Q13-nap-PG1601

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 4

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 5

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 6

R18 Changes to party contact information recorded

Free format text: ST27 STATUS EVENT CODE: A-5-5-R10-R18-OTH-X000 (AS PROVIDED BY THE NATIONAL OFFICE)

R18-X000 Changes to party contact information recorded

St.27 status event code: A-5-5-R10-R18-oth-X000