CN104053780A - 用于在微生物中生产蛋白质的来自枯草芽孢杆菌的核糖体启动子 - Google Patents

用于在微生物中生产蛋白质的来自枯草芽孢杆菌的核糖体启动子 Download PDF

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Publication number
CN104053780A
CN104053780A CN201280059922.9A CN201280059922A CN104053780A CN 104053780 A CN104053780 A CN 104053780A CN 201280059922 A CN201280059922 A CN 201280059922A CN 104053780 A CN104053780 A CN 104053780A
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China
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promoter
nucleic acid
seq
ribosomal
protein
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CN201280059922.9A
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Chinese (zh)
Inventor
C·邦焦尔尼
B·P·福克斯
A·范齐蒙耐德
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Danisco USA Inc
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Danisco USA Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21062—Subtilisin (3.4.21.62)

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201280059922.9A 2011-12-09 2012-12-06 用于在微生物中生产蛋白质的来自枯草芽孢杆菌的核糖体启动子 Pending CN104053780A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201161569202P 2011-12-09 2011-12-09
US61/569,202 2011-12-09
US201161577491P 2011-12-19 2011-12-19
US61/577,491 2011-12-19
PCT/US2012/068285 WO2013086219A1 (en) 2011-12-09 2012-12-06 Ribosomal promotors from b. subtilis for protein production in microorganisms

Publications (1)

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CN104053780A true CN104053780A (zh) 2014-09-17

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CN201280059922.9A Pending CN104053780A (zh) 2011-12-09 2012-12-06 用于在微生物中生产蛋白质的来自枯草芽孢杆菌的核糖体启动子

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US (1) US20140329309A1 (https=)
EP (1) EP2788491B1 (https=)
JP (1) JP6378089B2 (https=)
CN (1) CN104053780A (https=)
DK (1) DK2788491T3 (https=)
WO (1) WO2013086219A1 (https=)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107250365A (zh) * 2015-02-19 2017-10-13 丹尼斯科美国公司 增强的蛋白质表达
CN108441489A (zh) * 2018-04-09 2018-08-24 青岛蔚蓝生物集团有限公司 一种蛋白生产方法及高产碱性蛋白酶的枯草芽孢杆菌
CN108473994A (zh) * 2015-12-14 2018-08-31 巴斯夫欧洲公司 来自芽孢杆菌的经修饰的双向过氧化氢酶启动子
CN109071615A (zh) * 2016-03-04 2018-12-21 丹尼斯科美国公司 用于在微生物中产生蛋白质的工程化核糖体启动子
CN110004166A (zh) * 2018-01-05 2019-07-12 中国科学院天津工业生物技术研究所 高效表达分泌β-甘露聚糖酶的重组枯草芽孢杆菌菌株及其获得方法
CN111944808A (zh) * 2019-05-16 2020-11-17 武汉臻智生物科技有限公司 核糖体rna启动子在强启动表达非rna基因中的用途
CN113366108A (zh) * 2018-11-28 2021-09-07 丹尼斯科美国公司 新颖启动子序列及其增强芽孢杆菌属细胞蛋白生产的方法

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7025325B2 (ja) 2015-10-30 2022-02-24 ダニスコ・ユーエス・インク タンパク質発現の増強およびその方法
EP3380496B1 (en) 2015-12-23 2020-05-20 Danisco US Inc. Enhanced protein production and methods thereof
EP3472313B1 (en) 2016-06-17 2022-08-31 Danisco US Inc. Protease variants and uses thereof
DK3558026T3 (da) 2016-12-21 2026-01-12 Int N&H Denmark Aps Fremgangsmåder til anvendelse af termostabile serinproteaser
EP3559226B1 (en) 2016-12-21 2023-01-04 Danisco US Inc. Bacillus gibsonii-clade serine proteases
CN110312795B (zh) 2016-12-21 2024-07-23 丹尼斯科美国公司 蛋白酶变体及其用途
US20190367932A1 (en) 2017-01-23 2019-12-05 Danisco Us Inc. Methods and compositions for obtaining natural comptence in bacillus host cells
JP7231228B2 (ja) 2017-02-24 2023-03-01 ダニスコ・ユーエス・インク バチルス・リケニフォルミスにおける増加したタンパク質産生のための組成物及び方法
US11453871B2 (en) 2017-03-15 2022-09-27 Danisco Us Inc. Trypsin-like serine proteases and uses thereof
EP3596211B1 (en) 2017-03-15 2021-06-02 DuPont Nutrition Biosciences ApS Methods of using an archaeal serine protease
BR112019018969A2 (pt) 2017-03-15 2020-04-22 Dupont Nutrition Biosci Aps serina proteases similar à tripsina e usos das mesmas
DK3607097T3 (da) 2017-04-07 2023-09-18 Dupont Nutrition Biosci Aps Bacillus-værtsceller, der danner beta-galactosidaser og lactaser i fravær af p-nitrobenzylesterase-sideaktivitet
KR20200047668A (ko) 2017-09-13 2020-05-07 다니스코 유에스 인크. 바실러스에서 증가된 단백질 생산을 위한 변형된 5'-비번역 영역(utr) 서열
US20200354708A1 (en) 2017-11-29 2020-11-12 Danisco Us Inc. Subtilisin variants having improved stability
KR102715197B1 (ko) 2018-01-03 2024-10-08 다니스코 유에스 인크. 증가된 단백질 생산을 위한 돌연변이체 및 유전자 변형된 바실러스 세포 및 이의 방법
EP3799601A1 (en) 2018-06-19 2021-04-07 Danisco US Inc. Subtilisin variants
WO2019245704A1 (en) 2018-06-19 2019-12-26 Danisco Us Inc Subtilisin variants
US20210277374A1 (en) 2018-07-06 2021-09-09 Dupont Nutrition Biosciences Aps Xylanase-containing feed additives for cereal-based animal feed
EP3947656A1 (en) 2019-04-05 2022-02-09 Danisco US Inc. Methods for polynucleotide integration into the genome of bacillus using dual circular recombinant dna constructs and compositions thereof
KR102931179B1 (ko) 2019-04-05 2026-02-25 다니스코 유에스 인크. 선형 재조합 dna 작제물 및 이의 조성물을 이용하여 공여 dna 서열을 바실러스 게놈 내에 통합시키기 위한 방법
WO2021086606A1 (en) 2019-10-28 2021-05-06 Danisco Us Inc Microbial host cells for the production of heterologous cyanuric acid hydrolases and biuret hydrolases
US12612649B2 (en) 2019-11-19 2026-04-28 Danisco Us Inc. Selection marker free methods for modifying the genome of bacillus and compositions thereof
KR20220123019A (ko) 2019-12-31 2022-09-05 에어 프로틴 인코포레이티드 고단백질 식품 조성물
CN114945665A (zh) 2020-01-15 2022-08-26 丹尼斯科美国公司 用于增强地衣芽孢杆菌中蛋白产生的组合物和方法
EP4525615A2 (en) 2022-05-14 2025-03-26 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
KR20250054813A (ko) * 2022-09-02 2025-04-23 다니스코 유에스 인크. 그람 양성 세포에서 단백질 생산을 향상시키는 신규한 프로모터 및 5'-비번역 영역 돌연변이

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008148575A2 (en) * 2007-06-07 2008-12-11 Dsm Ip Assets B.V. Increased production of a target product via stabilization of mrna

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4760025A (en) 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
US5182204A (en) 1984-05-29 1993-01-26 Genencor International, Inc. Non-human carbonyl hydrolase mutants, vectors encoding same and hosts transformed with said vectors
US5264366A (en) 1984-05-29 1993-11-23 Genencor, Inc. Protease deficient bacillus
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5364770A (en) 1985-08-29 1994-11-15 Genencor International Inc. Heterologous polypeptides expressed in aspergillus
FR2625295B1 (fr) 1987-12-24 1990-04-13 Gaz De France Procede et appareil destines a assurer la combustion etagee d'un melange combustible-comburant diminuant la production d'oxydes d'azote
EP0351029B1 (en) 1988-07-15 2002-03-06 Genencor International, Inc. Novel glucose isomerase enzymes and their use
ES2322032T3 (es) 1990-12-10 2009-06-16 Genencor Int Sacrificacion mejorada de celulosa por clonacion y amplificacion del gen de beta-glucosidasa de trichoderma reesei.
CN101659926A (zh) 1994-06-30 2010-03-03 诺沃奇梅兹有限公司 非毒性、非产毒性、非致病性镰孢属表达系统及所用启动子和终止子
JP4307563B2 (ja) 1997-04-07 2009-08-05 ユニリーバー・ナームローゼ・ベンノートシャープ 糸状菌、特にアスペルギルス属に属する糸状菌のアグロバクテリウム媒介性形質転換
AR015977A1 (es) 1997-10-23 2001-05-30 Genencor Int Variantes de proteasa multiplemente substituida con carga neta alterada para su empleo en detergentes
US6287839B1 (en) 1997-11-19 2001-09-11 Genencor International, Inc. Cellulase producing actinomycetes, cellulase produced therefrom and method of producing same
US6562612B2 (en) 1997-11-19 2003-05-13 Genencor International, Inc. Cellulase producing actinomycetes, cellulase produced therefrom and method of producing same
US7718411B1 (en) 2004-08-05 2010-05-18 Danisco Us Inc. Trichoderma reesei G/11 xylanases with improved stability
FI108728B (fi) 1999-10-12 2002-03-15 Carbozyme Oy Menetelmä perheen G/11 ksylanaasien stabiilisuuden parantamiseksi ja optimaalisen pH-alueen muuttamiseksi
US6509185B1 (en) 2000-01-07 2003-01-21 Genencor International, Inc. Mutant aprE promotor
EP2374879A3 (en) 2002-04-22 2012-01-11 E. I. du Pont de Nemours and Company Promoter and plasmid system for genetic engineering
GB0422052D0 (en) 2004-10-04 2004-11-03 Dansico As Enzymes
EP1797178B1 (en) 2004-10-04 2012-09-12 DuPont Nutrition Biosciences ApS Citrobacter freundii phytase and homologues
GB0423139D0 (en) 2004-10-18 2004-11-17 Danisco Enzymes
EP2109356A2 (en) 2007-01-30 2009-10-21 Novozymes A/S Polypeptides having phytase activty and polynucleotides encoding same
ES2513217T3 (es) 2007-02-07 2014-10-24 Danisco Us, Inc., Genencor Division Fitasas de Buttiauxella sp. variantes que tienen propiedades alteradas
ES2527586T3 (es) 2007-11-20 2015-01-27 Danisco Us Inc. Variantes de glucoamilasa con propiedades modificadas
BRPI0910457B1 (pt) 2008-04-18 2020-12-29 Danisco Us Inc. variante de fitase, usos da mesma, composição enzimática, alimento e métodos de produção do mesmo, bem como para redução dos níveis de fósforo em estrume animal
MX364987B (es) 2008-06-06 2019-05-17 Danisco Us Inc Alfa-amilasas variantes de bacillus subtilis y metodos de uso de las mismas.
MX2010013113A (es) 2008-06-06 2010-12-21 Danisco Inc Variantes de alfa-amilasa geobacillus stearothermophilus con propiedades mejoradas.
MX2011004801A (es) 2008-11-11 2011-06-16 Danisco Inc Composiciones y metodos que comprenden una variante de subtilisina.
AR076312A1 (es) 2009-04-24 2011-06-01 Danisco Us Inc Proteasas con regiones pro modificadas
GB0922467D0 (en) 2009-04-24 2010-02-03 Danisco Feed supplement
AR076941A1 (es) * 2009-06-11 2011-07-20 Danisco Us Inc Cepa de bacillus para una mayor produccion de proteina
CN102575242B (zh) 2009-07-31 2015-03-25 丹尼斯科美国公司 具有经修饰的前原区域的蛋白酶

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008148575A2 (en) * 2007-06-07 2008-12-11 Dsm Ip Assets B.V. Increased production of a target product via stabilization of mrna

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
GEORGE C.STEWART,ET AL: "DNA sequence of the tandem ribosomal RNA promoter for B. subtilis operon rrnB", 《NUCLEIC ACIDS RESEARCH》 *
KEIKO KOGA,ET AL: "Construction of Bacillus subtilis strains carrying the transcriptional bgaB fusion with the promoter region of each rrn operon and their differential transcription during spore development", 《J. GEN. APPL. MICROBIOL》 *
KRASNY LIBOR ET AL: "An alternative strategy for bacterial ribosome synthesis: Bacillus subtilis rRNA transcription regulation", 《EMBO (EUROPEAN MOLECULAR BIOLOGY ORGANIZATION) JOURNAL》 *
LUKACSOVICH T， ET AL: "A FAMILY OF EXPRESSION VECTORS BASED ON THE RRNB P2 PROMOTER OF ESCHERICHIA COLI", 《JOURNAL OF BIOTECHNOLOGY》 *
REINDERT NIJLAND,ET AL: "Heterologous production and secretion of Clostridium perfringens -toxoid in closely related Gram-positive hosts", 《JOURNAL OF BIOTECHNOLOGY》 *

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107250365A (zh) * 2015-02-19 2017-10-13 丹尼斯科美国公司 增强的蛋白质表达
CN108473994A (zh) * 2015-12-14 2018-08-31 巴斯夫欧洲公司 来自芽孢杆菌的经修饰的双向过氧化氢酶启动子
CN108473994B (zh) * 2015-12-14 2023-09-01 巴斯夫欧洲公司 来自芽孢杆菌的经修饰的双向过氧化氢酶启动子
CN109071615A (zh) * 2016-03-04 2018-12-21 丹尼斯科美国公司 用于在微生物中产生蛋白质的工程化核糖体启动子
CN110004166A (zh) * 2018-01-05 2019-07-12 中国科学院天津工业生物技术研究所 高效表达分泌β-甘露聚糖酶的重组枯草芽孢杆菌菌株及其获得方法
CN108441489A (zh) * 2018-04-09 2018-08-24 青岛蔚蓝生物集团有限公司 一种蛋白生产方法及高产碱性蛋白酶的枯草芽孢杆菌
CN108441489B (zh) * 2018-04-09 2023-04-14 潍坊康地恩生物科技有限公司 一种蛋白生产方法及高产碱性蛋白酶的枯草芽孢杆菌
CN113366108A (zh) * 2018-11-28 2021-09-07 丹尼斯科美国公司 新颖启动子序列及其增强芽孢杆菌属细胞蛋白生产的方法
CN111944808A (zh) * 2019-05-16 2020-11-17 武汉臻智生物科技有限公司 核糖体rna启动子在强启动表达非rna基因中的用途
CN111944808B (zh) * 2019-05-16 2025-04-29 武汉合生科技有限公司 核糖体rna启动子在强启动表达非rna基因中的用途

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JP6378089B2 (ja) 2018-08-22
DK2788491T3 (en) 2019-04-01
JP2015500032A (ja) 2015-01-05
EP2788491B1 (en) 2019-01-23
WO2013086219A1 (en) 2013-06-13
EP2788491A1 (en) 2014-10-15
US20140329309A1 (en) 2014-11-06

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