CN104053780A - 用于在微生物中生产蛋白质的来自枯草芽孢杆菌的核糖体启动子 - Google Patents

用于在微生物中生产蛋白质的来自枯草芽孢杆菌的核糖体启动子 Download PDF

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Publication number
CN104053780A
CN104053780A CN201280059922.9A CN201280059922A CN104053780A CN 104053780 A CN104053780 A CN 104053780A CN 201280059922 A CN201280059922 A CN 201280059922A CN 104053780 A CN104053780 A CN 104053780A
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promoter
nucleic acid
seq
ribosomal
protein
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CN201280059922.9A
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Chinese (zh)
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C·邦焦尔尼
B·P·福克斯
A·范齐蒙耐德
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Danisco USA Inc
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Danisco USA Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21062—Subtilisin (3.4.21.62)

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201280059922.9A 2011-12-09 2012-12-06 用于在微生物中生产蛋白质的来自枯草芽孢杆菌的核糖体启动子 Pending CN104053780A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201161569202P 2011-12-09 2011-12-09
US61/569,202 2011-12-09
US201161577491P 2011-12-19 2011-12-19
US61/577,491 2011-12-19
PCT/US2012/068285 WO2013086219A1 (en) 2011-12-09 2012-12-06 Ribosomal promotors from b. subtilis for protein production in microorganisms

Publications (1)

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CN104053780A true CN104053780A (zh) 2014-09-17

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CN201280059922.9A Pending CN104053780A (zh) 2011-12-09 2012-12-06 用于在微生物中生产蛋白质的来自枯草芽孢杆菌的核糖体启动子

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US (1) US20140329309A1 (https=)
EP (1) EP2788491B1 (https=)
JP (1) JP6378089B2 (https=)
CN (1) CN104053780A (https=)
DK (1) DK2788491T3 (https=)
WO (1) WO2013086219A1 (https=)

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CN107250365A (zh) * 2015-02-19 2017-10-13 丹尼斯科美国公司 增强的蛋白质表达
CN108441489A (zh) * 2018-04-09 2018-08-24 青岛蔚蓝生物集团有限公司 一种蛋白生产方法及高产碱性蛋白酶的枯草芽孢杆菌
CN108473994A (zh) * 2015-12-14 2018-08-31 巴斯夫欧洲公司 来自芽孢杆菌的经修饰的双向过氧化氢酶启动子
CN109071615A (zh) * 2016-03-04 2018-12-21 丹尼斯科美国公司 用于在微生物中产生蛋白质的工程化核糖体启动子
CN110004166A (zh) * 2018-01-05 2019-07-12 中国科学院天津工业生物技术研究所 高效表达分泌β-甘露聚糖酶的重组枯草芽孢杆菌菌株及其获得方法
CN111944808A (zh) * 2019-05-16 2020-11-17 武汉臻智生物科技有限公司 核糖体rna启动子在强启动表达非rna基因中的用途
CN113366108A (zh) * 2018-11-28 2021-09-07 丹尼斯科美国公司 新颖启动子序列及其增强芽孢杆菌属细胞蛋白生产的方法

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EP3717643A1 (en) 2017-11-29 2020-10-07 Danisco US Inc. Subtilisin variants having improved stability
JP7280269B2 (ja) 2018-01-03 2023-05-23 ダニスコ・ユーエス・インク タンパク質の産生増加のための変異及び遺伝子改変バチルス属（ｂａｃｉｌｌｕｓ）細胞、並びにその方法
EP3799601A1 (en) 2018-06-19 2021-04-07 Danisco US Inc. Subtilisin variants
WO2019245704A1 (en) 2018-06-19 2019-12-26 Danisco Us Inc Subtilisin variants
BR112021000116A2 (pt) 2018-07-06 2021-04-06 Dupont Nutrition Biosciences Aps Aditivos de alimento contendo xilanase para alimento de animal à base de cereal
US20220177923A1 (en) 2019-04-05 2022-06-09 Danisco Us Inc Methods for integrating a donor DNA sequence into the genome of bacillus using linear recombinant DNA constructs and compositions thereof
CA3136113A1 (en) 2019-04-05 2020-10-08 Danisco Us Inc. Methods for polynucleotide integration into the genome of bacillus using dual circular recombinant dna constructs and compositions thereof
CA3155372A1 (en) 2019-10-28 2021-05-06 Sharief Barends Microbial host cells for the production of heterologous cyanuric acid hydrolases and biuret hydrolases
JP2023502967A (ja) 2019-11-19 2023-01-26 ダニスコ・ユーエス・インク バチルス（Ｂａｃｉｌｌｕｓ）のゲノムを改変するための、選択マーカーを使用しない方法及びその組成物
IL294248A (en) * 2019-12-31 2022-08-01 Air Protein Inc High protein food compositions
CN114945665A (zh) 2020-01-15 2022-08-26 丹尼斯科美国公司 用于增强地衣芽孢杆菌中蛋白产生的组合物和方法
CN119630280A (zh) 2022-05-14 2025-03-14 诺维信公司 用于预防、处理、抑制和/或消除植物病原性侵染和感染的组合物和方法
KR20250054813A (ko) 2022-09-02 2025-04-23 다니스코 유에스 인크. 그람 양성 세포에서 단백질 생산을 향상시키는 신규한 프로모터 및 5'-비번역 영역 돌연변이

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Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107250365A (zh) * 2015-02-19 2017-10-13 丹尼斯科美国公司 增强的蛋白质表达
CN108473994A (zh) * 2015-12-14 2018-08-31 巴斯夫欧洲公司 来自芽孢杆菌的经修饰的双向过氧化氢酶启动子
CN108473994B (zh) * 2015-12-14 2023-09-01 巴斯夫欧洲公司 来自芽孢杆菌的经修饰的双向过氧化氢酶启动子
CN109071615A (zh) * 2016-03-04 2018-12-21 丹尼斯科美国公司 用于在微生物中产生蛋白质的工程化核糖体启动子
CN110004166A (zh) * 2018-01-05 2019-07-12 中国科学院天津工业生物技术研究所 高效表达分泌β-甘露聚糖酶的重组枯草芽孢杆菌菌株及其获得方法
CN108441489A (zh) * 2018-04-09 2018-08-24 青岛蔚蓝生物集团有限公司 一种蛋白生产方法及高产碱性蛋白酶的枯草芽孢杆菌
CN108441489B (zh) * 2018-04-09 2023-04-14 潍坊康地恩生物科技有限公司 一种蛋白生产方法及高产碱性蛋白酶的枯草芽孢杆菌
CN113366108A (zh) * 2018-11-28 2021-09-07 丹尼斯科美国公司 新颖启动子序列及其增强芽孢杆菌属细胞蛋白生产的方法
CN111944808A (zh) * 2019-05-16 2020-11-17 武汉臻智生物科技有限公司 核糖体rna启动子在强启动表达非rna基因中的用途
CN111944808B (zh) * 2019-05-16 2025-04-29 武汉合生科技有限公司 核糖体rna启动子在强启动表达非rna基因中的用途

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EP2788491B1 (en) 2019-01-23
JP2015500032A (ja) 2015-01-05
WO2013086219A1 (en) 2013-06-13
US20140329309A1 (en) 2014-11-06
EP2788491A1 (en) 2014-10-15
JP6378089B2 (ja) 2018-08-22
DK2788491T3 (en) 2019-04-01

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