CN104053780A - 用于在微生物中生产蛋白质的来自枯草芽孢杆菌的核糖体启动子 - Google Patents

用于在微生物中生产蛋白质的来自枯草芽孢杆菌的核糖体启动子 Download PDF

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Publication number
CN104053780A
CN104053780A CN201280059922.9A CN201280059922A CN104053780A CN 104053780 A CN104053780 A CN 104053780A CN 201280059922 A CN201280059922 A CN 201280059922A CN 104053780 A CN104053780 A CN 104053780A
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China
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promoter
nucleic acid
seq
ribosomal
protein
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CN201280059922.9A
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Chinese (zh)
Inventor
C·邦焦尔尼
B·P·福克斯
A·范齐蒙耐德
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Danisco USA Inc
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Danisco USA Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21062—Subtilisin (3.4.21.62)

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201280059922.9A 2011-12-09 2012-12-06 用于在微生物中生产蛋白质的来自枯草芽孢杆菌的核糖体启动子 Pending CN104053780A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201161569202P 2011-12-09 2011-12-09
US61/569,202 2011-12-09
US201161577491P 2011-12-19 2011-12-19
US61/577,491 2011-12-19
PCT/US2012/068285 WO2013086219A1 (en) 2011-12-09 2012-12-06 Ribosomal promotors from b. subtilis for protein production in microorganisms

Publications (1)

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CN104053780A true CN104053780A (zh) 2014-09-17

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CN201280059922.9A Pending CN104053780A (zh) 2011-12-09 2012-12-06 用于在微生物中生产蛋白质的来自枯草芽孢杆菌的核糖体启动子

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US (1) US20140329309A1 (enExample)
EP (1) EP2788491B1 (enExample)
JP (1) JP6378089B2 (enExample)
CN (1) CN104053780A (enExample)
DK (1) DK2788491T3 (enExample)
WO (1) WO2013086219A1 (enExample)

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CN107250365A (zh) * 2015-02-19 2017-10-13 丹尼斯科美国公司 增强的蛋白质表达
CN108441489A (zh) * 2018-04-09 2018-08-24 青岛蔚蓝生物集团有限公司 一种蛋白生产方法及高产碱性蛋白酶的枯草芽孢杆菌
CN108473994A (zh) * 2015-12-14 2018-08-31 巴斯夫欧洲公司 来自芽孢杆菌的经修饰的双向过氧化氢酶启动子
CN109071615A (zh) * 2016-03-04 2018-12-21 丹尼斯科美国公司 用于在微生物中产生蛋白质的工程化核糖体启动子
CN110004166A (zh) * 2018-01-05 2019-07-12 中国科学院天津工业生物技术研究所 高效表达分泌β-甘露聚糖酶的重组枯草芽孢杆菌菌株及其获得方法
CN111944808A (zh) * 2019-05-16 2020-11-17 武汉臻智生物科技有限公司 核糖体rna启动子在强启动表达非rna基因中的用途
CN113366108A (zh) * 2018-11-28 2021-09-07 丹尼斯科美国公司 新颖启动子序列及其增强芽孢杆菌属细胞蛋白生产的方法

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EP3559227B1 (en) 2016-12-21 2025-02-19 Danisco US Inc. Protease variants and uses thereof
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RU2019132448A (ru) 2017-03-15 2021-04-15 ДюПон НЬЮТРИШН БАЙОСАЙЕНСИЗ АпС Способы применения сериновой протеазы архей
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CN111094576A (zh) 2017-09-13 2020-05-01 丹尼斯科美国公司 用于增加芽胞杆菌属中蛋白质产生的经修饰的5′-非翻译区(utr)序列
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US11414643B2 (en) 2018-01-03 2022-08-16 Danisco Us Inc. Mutant and genetically modified Bacillus cells and methods thereof for increased protein production
US12415996B2 (en) 2018-06-19 2025-09-16 Danisco Us Inc. Subtilisin variants
EP3799601A1 (en) 2018-06-19 2021-04-07 Danisco US Inc. Subtilisin variants
EP3818154A1 (en) 2018-07-06 2021-05-12 DuPont Nutrition Biosciences ApS Xylanase-containing feed additives for cereal-based animal feed
MX2021012158A (es) 2019-04-05 2022-01-06 Danisco Us Inc Métodos para la integración de una secuencia de adn donante en el genoma de bacillus usando constructos de adn recombinante lineal y composiciones de los mismos.
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WO2021086606A1 (en) 2019-10-28 2021-05-06 Danisco Us Inc Microbial host cells for the production of heterologous cyanuric acid hydrolases and biuret hydrolases
CN114981428A (zh) 2019-11-19 2022-08-30 丹尼斯科美国公司 用于修饰芽孢杆菌属基因组的无选择标记方法及其组合物
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KR20220127844A (ko) 2020-01-15 2022-09-20 다니스코 유에스 인크. 바실러스 리체니포르미스에서 단백질 생산을 향상시키기 위한 조성물 및 방법
EP4525615A2 (en) 2022-05-14 2025-03-26 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
JP2025529135A (ja) * 2022-09-02 2025-09-04 ダニスコ・ユーエス・インク グラム陽性細胞におけるタンパク質産生を強化する新規なプロモーター及び５’非翻訳領域の変異

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Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107250365A (zh) * 2015-02-19 2017-10-13 丹尼斯科美国公司 增强的蛋白质表达
CN108473994A (zh) * 2015-12-14 2018-08-31 巴斯夫欧洲公司 来自芽孢杆菌的经修饰的双向过氧化氢酶启动子
CN108473994B (zh) * 2015-12-14 2023-09-01 巴斯夫欧洲公司 来自芽孢杆菌的经修饰的双向过氧化氢酶启动子
CN109071615A (zh) * 2016-03-04 2018-12-21 丹尼斯科美国公司 用于在微生物中产生蛋白质的工程化核糖体启动子
CN110004166A (zh) * 2018-01-05 2019-07-12 中国科学院天津工业生物技术研究所 高效表达分泌β-甘露聚糖酶的重组枯草芽孢杆菌菌株及其获得方法
CN108441489A (zh) * 2018-04-09 2018-08-24 青岛蔚蓝生物集团有限公司 一种蛋白生产方法及高产碱性蛋白酶的枯草芽孢杆菌
CN108441489B (zh) * 2018-04-09 2023-04-14 潍坊康地恩生物科技有限公司 一种蛋白生产方法及高产碱性蛋白酶的枯草芽孢杆菌
CN113366108A (zh) * 2018-11-28 2021-09-07 丹尼斯科美国公司 新颖启动子序列及其增强芽孢杆菌属细胞蛋白生产的方法
CN111944808A (zh) * 2019-05-16 2020-11-17 武汉臻智生物科技有限公司 核糖体rna启动子在强启动表达非rna基因中的用途
CN111944808B (zh) * 2019-05-16 2025-04-29 武汉合生科技有限公司 核糖体rna启动子在强启动表达非rna基因中的用途

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JP6378089B2 (ja) 2018-08-22
JP2015500032A (ja) 2015-01-05
EP2788491B1 (en) 2019-01-23
US20140329309A1 (en) 2014-11-06
EP2788491A1 (en) 2014-10-15
WO2013086219A1 (en) 2013-06-13
DK2788491T3 (en) 2019-04-01

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