DK2788491T3 - RIBOSOMAL PROMOTERS FROM B. SUBTILIS FOR PROTEIN PRODUCTION IN MICRO-ORGANISMS - Google Patents
RIBOSOMAL PROMOTERS FROM B. SUBTILIS FOR PROTEIN PRODUCTION IN MICRO-ORGANISMS Download PDFInfo
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- DK2788491T3 DK2788491T3 DK12806777.4T DK12806777T DK2788491T3 DK 2788491 T3 DK2788491 T3 DK 2788491T3 DK 12806777 T DK12806777 T DK 12806777T DK 2788491 T3 DK2788491 T3 DK 2788491T3
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21062—Subtilisin (3.4.21.62)
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
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- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
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| US201161577491P | 2011-12-19 | 2011-12-19 | |
| PCT/US2012/068285 WO2013086219A1 (en) | 2011-12-09 | 2012-12-06 | Ribosomal promotors from b. subtilis for protein production in microorganisms |
Publications (1)
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| DK2788491T3 true DK2788491T3 (en) | 2019-04-01 |
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| US (1) | US20140329309A1 (enExample) |
| EP (1) | EP2788491B1 (enExample) |
| JP (1) | JP6378089B2 (enExample) |
| CN (1) | CN104053780A (enExample) |
| DK (1) | DK2788491T3 (enExample) |
| WO (1) | WO2013086219A1 (enExample) |
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| US20180030456A1 (en) * | 2015-02-19 | 2018-02-01 | Danisco Us Inc. | Enhanced protein expression |
| KR20180069057A (ko) | 2015-10-30 | 2018-06-22 | 다니스코 유에스 인크. | 향상된 단백질 발현 및 이의 방법 |
| CN108473994B (zh) * | 2015-12-14 | 2023-09-01 | 巴斯夫欧洲公司 | 来自芽孢杆菌的经修饰的双向过氧化氢酶启动子 |
| US20180371445A1 (en) | 2015-12-23 | 2018-12-27 | Danisco Us Inc. | Enhanced protein production and methods thereof |
| KR20180117166A (ko) | 2016-03-04 | 2018-10-26 | 다니스코 유에스 인크. | 미생물에서의 단백질 생산을 위한 유전자 조작 리보솜 프로모터 |
| CA3027745A1 (en) | 2016-06-17 | 2017-12-21 | Danisco Us Inc. | Protease variants and uses thereof |
| EP4644560A3 (en) | 2016-12-21 | 2026-04-08 | International N&H Denmark ApS | Methods of using thermostable serine proteases |
| CN110312794B (zh) | 2016-12-21 | 2024-04-12 | 丹尼斯科美国公司 | 吉氏芽孢杆菌进化枝丝氨酸蛋白酶 |
| EP4520820A3 (en) | 2016-12-21 | 2025-04-16 | Danisco Us Inc | Protease variants and uses thereof |
| WO2018136459A1 (en) | 2017-01-23 | 2018-07-26 | Danisco Us Inc. | Methods and compositions for obtaining natural competence in bacillus host cells |
| EP3585910B1 (en) | 2017-02-24 | 2024-03-20 | Danisco US Inc. | Compositions and methods for increased protein production in bacillus licheniformis |
| WO2018169780A1 (en) | 2017-03-15 | 2018-09-20 | Dupont Nutrition Biosciences Aps | Methods of using an archaeal serine protease |
| CN110621778A (zh) | 2017-03-15 | 2019-12-27 | 丹尼斯科美国公司 | 胰蛋白酶样丝氨酸蛋白酶及其用途 |
| WO2018169784A1 (en) | 2017-03-15 | 2018-09-20 | Dupont Nutrition Biosciences Aps | Trypsin-like serine proteases and uses thereof cross-reference to related application |
| FI3607097T3 (fi) | 2017-04-07 | 2023-09-11 | Dupont Nutrition Biosci Aps | BEETA-GALAKTOSIDAASEJA JA LAKTAASEJA ILMAN p-NITROBENTSYYLIESTERASISIVUAKTIIVISUUTTA TUOTTAVIA BACILLUS-ISÄNTÄSOLUJA |
| US20210032639A1 (en) | 2017-09-13 | 2021-02-04 | Danisco Us Inc. | Modified 5'-untranslated region (utr) sequences for increased protein production in bacillus |
| EP3717643A1 (en) | 2017-11-29 | 2020-10-07 | Danisco US Inc. | Subtilisin variants having improved stability |
| CN111670244B (zh) | 2018-01-03 | 2023-08-01 | 丹尼斯科美国公司 | 突变的和遗传修饰的芽孢杆菌属细胞及其用于增加蛋白质产生的方法 |
| CN110004166A (zh) * | 2018-01-05 | 2019-07-12 | 中国科学院天津工业生物技术研究所 | 高效表达分泌β-甘露聚糖酶的重组枯草芽孢杆菌菌株及其获得方法 |
| CN108441489B (zh) * | 2018-04-09 | 2023-04-14 | 潍坊康地恩生物科技有限公司 | 一种蛋白生产方法及高产碱性蛋白酶的枯草芽孢杆菌 |
| WO2019245704A1 (en) | 2018-06-19 | 2019-12-26 | Danisco Us Inc | Subtilisin variants |
| US20210363470A1 (en) | 2018-06-19 | 2021-11-25 | Danisco Us Inc | Subtilisin variants |
| BR112021000116A2 (pt) | 2018-07-06 | 2021-04-06 | Dupont Nutrition Biosciences Aps | Aditivos de alimento contendo xilanase para alimento de animal à base de cereal |
| US11781147B2 (en) * | 2018-11-28 | 2023-10-10 | Danisco Us Inc. | Promoter sequences and methods thereof for enhanced protein production in Bacillus cells |
| JP2022526982A (ja) | 2019-04-05 | 2022-05-27 | ダニスコ・ユーエス・インク | 線状組換えＤＮＡコンストラクトを使用してバチルス（Ｂａｃｉｌｌｕｓ）のゲノムにドナーＤＮＡ配列を組み込むための方法及びその組成物 |
| CA3136113A1 (en) | 2019-04-05 | 2020-10-08 | Danisco Us Inc. | Methods for polynucleotide integration into the genome of bacillus using dual circular recombinant dna constructs and compositions thereof |
| CN111944808B (zh) * | 2019-05-16 | 2025-04-29 | 武汉合生科技有限公司 | 核糖体rna启动子在强启动表达非rna基因中的用途 |
| WO2021086606A1 (en) | 2019-10-28 | 2021-05-06 | Danisco Us Inc | Microbial host cells for the production of heterologous cyanuric acid hydrolases and biuret hydrolases |
| WO2021101950A1 (en) | 2019-11-19 | 2021-05-27 | Danisco Us Inc | Selection marker free methods for modifying the genome of bacillus and compositions thereof |
| PH12022551477A1 (en) * | 2019-12-31 | 2023-11-29 | Air Protein Inc | High protein food compositions |
| JP7787077B2 (ja) | 2020-01-15 | 2025-12-16 | ダニスコ・ユーエス・インク | バチルス・リケニフォルミス（ｂａｃｉｌｌｕｓ ｌｉｃｈｅｎｉｆｏｒｍｉｓ）における強化したタンパク質産生のための組成物及び方法 |
| PE20230443A1 (es) | 2020-03-02 | 2023-03-08 | Air Protein Inc | Composiciones estructuradas analogas a la carne con alto contenido de proteinas |
| US20250311734A1 (en) | 2022-05-14 | 2025-10-09 | Novozymes A/S | Compositions and Methods for Preventing, Treating, Suppressing and/or Eliminatting Phytopathogenic Infestations and Infections |
| US20260092297A1 (en) * | 2022-09-02 | 2026-04-02 | Danisco Us Inc. | Novel promoter and 5'-untranslated region mutations enhancing protein production in gram-positive cells |
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| US4760025A (en) | 1984-05-29 | 1988-07-26 | Genencor, Inc. | Modified enzymes and methods for making same |
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| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US5364770A (en) | 1985-08-29 | 1994-11-15 | Genencor International Inc. | Heterologous polypeptides expressed in aspergillus |
| FR2625295B1 (fr) | 1987-12-24 | 1990-04-13 | Gaz De France | Procede et appareil destines a assurer la combustion etagee d'un melange combustible-comburant diminuant la production d'oxydes d'azote |
| EP0351029B1 (en) | 1988-07-15 | 2002-03-06 | Genencor International, Inc. | Novel glucose isomerase enzymes and their use |
| DK0562003T4 (en) | 1990-12-10 | 2015-07-13 | Danisco Us Inc | Improved saccharification of cellulose by cloning and amplification of.-Glucosidase gene from Tricodermareesei |
| ATE294871T1 (de) | 1994-06-30 | 2005-05-15 | Novozymes Biotech Inc | Nicht-toxisches, nicht-toxigenes, nicht- pathogenes fusarium expressionssystem und darin zu verwendende promotoren und terminatoren |
| JP4307563B2 (ja) | 1997-04-07 | 2009-08-05 | ユニリーバー・ナームローゼ・ベンノートシャープ | 糸状菌、特にアスペルギルス属に属する糸状菌のアグロバクテリウム媒介性形質転換 |
| AR016969A1 (es) | 1997-10-23 | 2001-08-01 | Procter & Gamble | VARIANTE DE PROTEASA, ADN, VECTOR DE EXPRESIoN, MICROORGANISMO HUESPED, COMPOSICIoN DE LIMPIEZA, ALIMENTO PARA ANIMALES Y COMPOSICIoN PARA TRATAR UN TEXTIL |
| US6287839B1 (en) | 1997-11-19 | 2001-09-11 | Genencor International, Inc. | Cellulase producing actinomycetes, cellulase produced therefrom and method of producing same |
| US6562612B2 (en) | 1997-11-19 | 2003-05-13 | Genencor International, Inc. | Cellulase producing actinomycetes, cellulase produced therefrom and method of producing same |
| FI108728B (fi) | 1999-10-12 | 2002-03-15 | Carbozyme Oy | Menetelmä perheen G/11 ksylanaasien stabiilisuuden parantamiseksi ja optimaalisen pH-alueen muuttamiseksi |
| US7718411B1 (en) | 2004-08-05 | 2010-05-18 | Danisco Us Inc. | Trichoderma reesei G/11 xylanases with improved stability |
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| GB0422052D0 (en) | 2004-10-04 | 2004-11-03 | Dansico As | Enzymes |
| WO2006038128A2 (en) | 2004-10-04 | 2006-04-13 | Danisco A/S | Citrobacter freundii phytase and homologues |
| GB0423139D0 (en) | 2004-10-18 | 2004-11-17 | Danisco | Enzymes |
| EP2109356A2 (en) | 2007-01-30 | 2009-10-21 | Novozymes A/S | Polypeptides having phytase activty and polynucleotides encoding same |
| PL2118276T3 (pl) | 2007-02-07 | 2016-01-29 | Danisco Us Inc | Hydroliza skrobi za pomocą fitazy z alfa-amylazą |
| WO2008148575A2 (en) * | 2007-06-07 | 2008-12-11 | Dsm Ip Assets B.V. | Increased production of a target product via stabilization of mrna |
| DK3323889T3 (da) | 2007-11-20 | 2020-06-29 | Danisco Us Inc | Glucoamylasevarianter med ændrede egenskaber |
| WO2009129489A2 (en) | 2008-04-18 | 2009-10-22 | Danisco Us Inc., Genencor Division | Buttiauxella sp. phytase variants |
| WO2009149130A2 (en) | 2008-06-06 | 2009-12-10 | Danisco Us Inc. | Geobacillus stearothermophilus alpha-amylase (amys) variants with improved properties |
| WO2009149419A2 (en) | 2008-06-06 | 2009-12-10 | Danisco Us Inc. | Variant alpha-amylases from bacillus subtilis and methods of use, thereof |
| WO2010056635A1 (en) | 2008-11-11 | 2010-05-20 | Danisco Us Inc. | Compositions and methods comprising a subtilisin variant |
| WO2010123754A1 (en) | 2009-04-24 | 2010-10-28 | Danisco Us Inc. | Proteases with modified pro regions |
| GB0922467D0 (en) | 2009-04-24 | 2010-02-03 | Danisco | Feed supplement |
| AR076941A1 (es) * | 2009-06-11 | 2011-07-20 | Danisco Us Inc | Cepa de bacillus para una mayor produccion de proteina |
| EP2459714A1 (en) | 2009-07-31 | 2012-06-06 | Danisco US Inc. | Proteases with modified pre-pro regions |
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- 2012-12-06 WO PCT/US2012/068285 patent/WO2013086219A1/en not_active Ceased
- 2012-12-06 JP JP2014546087A patent/JP6378089B2/ja active Active
- 2012-12-06 DK DK12806777.4T patent/DK2788491T3/en active
- 2012-12-06 CN CN201280059922.9A patent/CN104053780A/zh active Pending
- 2012-12-06 EP EP12806777.4A patent/EP2788491B1/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| JP6378089B2 (ja) | 2018-08-22 |
| WO2013086219A1 (en) | 2013-06-13 |
| EP2788491B1 (en) | 2019-01-23 |
| US20140329309A1 (en) | 2014-11-06 |
| EP2788491A1 (en) | 2014-10-15 |
| JP2015500032A (ja) | 2015-01-05 |
| CN104053780A (zh) | 2014-09-17 |
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