JP6378089B2 - 微生物におけるタンパク質産生のための、Ｂ．ズブチリス（Ｂ．ｓｕｂｔｉｌｉｓ）からのリボソームプロモーター - Google Patents

微生物におけるタンパク質産生のための、Ｂ．ズブチリス（Ｂ．ｓｕｂｔｉｌｉｓ）からのリボソームプロモーター Download PDF

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JP6378089B2
JP6378089B2 JP2014546087A JP2014546087A JP6378089B2 JP 6378089 B2 JP6378089 B2 JP 6378089B2 JP 2014546087 A JP2014546087 A JP 2014546087A JP 2014546087 A JP2014546087 A JP 2014546087A JP 6378089 B2 JP6378089 B2 JP 6378089B2
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JP2015500032A (ja
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ボンジョルニ、クリスティーナ
フォックス、ブライアン・ピー
ヴァン・キムネーデ・アニタ
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ダニスコ・ユーエス・インク
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21062—Subtilisin (3.4.21.62)

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  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
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  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
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  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2014546087A 2011-12-09 2012-12-06 微生物におけるタンパク質産生のための、Ｂ．ズブチリス（Ｂ．ｓｕｂｔｉｌｉｓ）からのリボソームプロモーター Active JP6378089B2 (ja)

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US201161569202P 2011-12-09 2011-12-09
US61/569,202 2011-12-09
US201161577491P 2011-12-19 2011-12-19
US61/577,491 2011-12-19
PCT/US2012/068285 WO2013086219A1 (en) 2011-12-09 2012-12-06 Ribosomal promotors from b. subtilis for protein production in microorganisms

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JP2015500032A JP2015500032A (ja) 2015-01-05
JP2015500032A5 JP2015500032A5 (enExample) 2016-01-28
JP6378089B2 true JP6378089B2 (ja) 2018-08-22

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US (1) US20140329309A1 (enExample)
EP (1) EP2788491B1 (enExample)
JP (1) JP6378089B2 (enExample)
CN (1) CN104053780A (enExample)
DK (1) DK2788491T3 (enExample)
WO (1) WO2013086219A1 (enExample)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3259358B1 (en) * 2015-02-19 2024-06-19 Danisco US Inc. Enhanced protein expression
WO2017075195A1 (en) 2015-10-30 2017-05-04 Danisco Us Inc Enhanced protein expression and methods thereof
MX391716B (es) * 2015-12-14 2025-03-21 Basf Se Promotor de catalasa modificado bidireccional de bacilo.
DK3380496T3 (da) 2015-12-23 2020-08-24 Danisco Us Inc Forbedret proteinproduktion og fremgangsmåder hertil
EP3419991B1 (en) * 2016-03-04 2022-10-26 Danisco US Inc. Engineered ribosomal promoters for protein production in microorganisms
CA3027745A1 (en) 2016-06-17 2017-12-21 Danisco Us Inc. Protease variants and uses thereof
WO2018118917A1 (en) 2016-12-21 2018-06-28 Danisco Us Inc. Protease variants and uses thereof
CN110312794B (zh) 2016-12-21 2024-04-12 丹尼斯科美国公司 吉氏芽孢杆菌进化枝丝氨酸蛋白酶
RU2771261C2 (ru) 2016-12-21 2022-04-29 ДюПон НЬЮТРИШН БАЙОСАЙЕНСИЗ АпС Способы применения термостабильных сериновых протеаз
US20190367932A1 (en) 2017-01-23 2019-12-05 Danisco Us Inc. Methods and compositions for obtaining natural comptence in bacillus host cells
US11866713B2 (en) 2017-02-24 2024-01-09 Danisco Us Inc. Compositions and methods for increased protein production in bacillus licheniformis
CN110621777A (zh) 2017-03-15 2019-12-27 杜邦营养生物科学有限公司 胰蛋白酶样丝氨酸蛋白酶及其用途
BR112019018983A2 (pt) 2017-03-15 2020-04-14 Dupont Nutrition Biosci Aps métodos de uso de uma serina protease archaeal
WO2018169750A1 (en) 2017-03-15 2018-09-20 Danisco Us Inc Trypsin-like serine proteases and uses thereof
KR102533803B1 (ko) 2017-04-07 2023-05-18 듀폰 뉴트리션 바이오사이언시즈 에이피에스 p-니트로벤질에스터라제 부활성의 부재 하에 β-갈락토시다제 및 락타제를 생산하는 바실러스 숙주 세포
KR20200047668A (ko) 2017-09-13 2020-05-07 다니스코 유에스 인크. 바실러스에서 증가된 단백질 생산을 위한 변형된 5'-비번역 영역(utr) 서열
WO2019108599A1 (en) 2017-11-29 2019-06-06 Danisco Us Inc Subtilisin variants having improved stability
DK3735478T5 (da) 2018-01-03 2024-08-26 Danisco Us Inc Mutante og genmodificerede bacillus-celler og fremgangsmåder dertil til forøget proteinproduktion
CN110004166A (zh) * 2018-01-05 2019-07-12 中国科学院天津工业生物技术研究所 高效表达分泌β-甘露聚糖酶的重组枯草芽孢杆菌菌株及其获得方法
CN108441489B (zh) * 2018-04-09 2023-04-14 潍坊康地恩生物科技有限公司 一种蛋白生产方法及高产碱性蛋白酶的枯草芽孢杆菌
EP3810767A1 (en) 2018-06-19 2021-04-28 Danisco US Inc. Subtilisin variants
EP3799601A1 (en) 2018-06-19 2021-04-07 Danisco US Inc. Subtilisin variants
US20210277374A1 (en) 2018-07-06 2021-09-09 Dupont Nutrition Biosciences Aps Xylanase-containing feed additives for cereal-based animal feed
KR20210096629A (ko) 2018-11-28 2021-08-05 다니스코 유에스 인크. 바실러스 세포에서의 향상된 단백질 생산을 위한 신규 프로모터 서열 및 이의 방법
MX2021012158A (es) 2019-04-05 2022-01-06 Danisco Us Inc Métodos para la integración de una secuencia de adn donante en el genoma de bacillus usando constructos de adn recombinante lineal y composiciones de los mismos.
MX2021012157A (es) 2019-04-05 2022-01-06 Danisco Us Inc Métodos para la integración de polinucleótidos en el genoma de bacillus usando constructos de adn recombinante circular dual y composiciones de los mismos.
CN111944808B (zh) * 2019-05-16 2025-04-29 武汉合生科技有限公司 核糖体rna启动子在强启动表达非rna基因中的用途
CA3155372A1 (en) 2019-10-28 2021-05-06 Sharief Barends Microbial host cells for the production of heterologous cyanuric acid hydrolases and biuret hydrolases
JP2023502967A (ja) 2019-11-19 2023-01-26 ダニスコ・ユーエス・インク バチルス（Ｂａｃｉｌｌｕｓ）のゲノムを改変するための、選択マーカーを使用しない方法及びその組成物
IL322673A (en) * 2019-12-31 2025-10-01 Air Protein Inc High-protein food compositions
WO2021146411A1 (en) 2020-01-15 2021-07-22 Danisco Us Inc Compositions and methods for enhanced protein production in bacillus licheniformis
AU2023272468A1 (en) 2022-05-14 2024-11-14 Novonesis Plant Biosolutions A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
CN120129752A (zh) * 2022-09-02 2025-06-10 丹尼斯科美国公司 增强革兰氏阳性细胞中的蛋白质生产的新型启动子和5′-非翻译区突变

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4760025A (en) 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
US5182204A (en) 1984-05-29 1993-01-26 Genencor International, Inc. Non-human carbonyl hydrolase mutants, vectors encoding same and hosts transformed with said vectors
US5264366A (en) 1984-05-29 1993-11-23 Genencor, Inc. Protease deficient bacillus
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5364770A (en) 1985-08-29 1994-11-15 Genencor International Inc. Heterologous polypeptides expressed in aspergillus
FR2625295B1 (fr) 1987-12-24 1990-04-13 Gaz De France Procede et appareil destines a assurer la combustion etagee d'un melange combustible-comburant diminuant la production d'oxydes d'azote
EP0351029B1 (en) 1988-07-15 2002-03-06 Genencor International, Inc. Novel glucose isomerase enzymes and their use
DK1225227T3 (da) 1990-12-10 2009-06-22 Genencor Int Forbedret forsukring af cellulose ved kloning og amplifikation af beta-glucosidase genet af trichoderma
WO1996000787A1 (en) 1994-06-30 1996-01-11 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein
DE69822142T2 (de) 1997-04-07 2005-03-03 Unilever N.V. Transformation von schimmeln durch agrobacterium, insbesondere von der gattung aspergillus
AR015977A1 (es) 1997-10-23 2001-05-30 Genencor Int Variantes de proteasa multiplemente substituida con carga neta alterada para su empleo en detergentes
US6287839B1 (en) 1997-11-19 2001-09-11 Genencor International, Inc. Cellulase producing actinomycetes, cellulase produced therefrom and method of producing same
US6562612B2 (en) 1997-11-19 2003-05-13 Genencor International, Inc. Cellulase producing actinomycetes, cellulase produced therefrom and method of producing same
US7718411B1 (en) 2004-08-05 2010-05-18 Danisco Us Inc. Trichoderma reesei G/11 xylanases with improved stability
FI108728B (fi) 1999-10-12 2002-03-15 Carbozyme Oy Menetelmä perheen G/11 ksylanaasien stabiilisuuden parantamiseksi ja optimaalisen pH-alueen muuttamiseksi
US6509185B1 (en) 2000-01-07 2003-01-21 Genencor International, Inc. Mutant aprE promotor
CA2481414C (en) 2002-04-22 2013-04-09 E.I. Du Pont De Nemours And Company Promoter and plasmid system for genetic engineering
AU2005290934C1 (en) 2004-10-04 2013-05-09 Dupont Nutrition Biosciences Aps Citrobacter freundii phytase and homologues
GB0422052D0 (en) 2004-10-04 2004-11-03 Dansico As Enzymes
GB0423139D0 (en) 2004-10-18 2004-11-17 Danisco Enzymes
EP2109356A2 (en) 2007-01-30 2009-10-21 Novozymes A/S Polypeptides having phytase activty and polynucleotides encoding same
CN101688192A (zh) 2007-02-07 2010-03-31 丹尼斯科美国公司 用植酸酶和α-淀粉酶进行淀粉水解
WO2008148575A2 (en) * 2007-06-07 2008-12-11 Dsm Ip Assets B.V. Increased production of a target product via stabilization of mrna
MX2010005461A (es) 2007-11-20 2010-06-02 Danisco Us Inc Variantes de glucoamilasa con propiedades alteradas.
ES2835955T3 (es) 2008-04-18 2021-06-23 Danisco Us Inc Variantes de fitasa de Buttiauxella sp.
DK2297313T3 (en) 2008-06-06 2015-06-08 Danisco Us Inc ALPHA AMYLASE VARIETIES OF BACILLUS SUBTILIS AND METHODS OF USING IT
CN105483099B (zh) 2008-06-06 2020-06-23 丹尼斯科美国公司 具有改良特性的嗜热脂肪土芽孢杆菌α-淀粉酶(AMYS)变体
US8530219B2 (en) 2008-11-11 2013-09-10 Danisco Us Inc. Compositions and methods comprising a subtilisin variant
WO2010123754A1 (en) 2009-04-24 2010-10-28 Danisco Us Inc. Proteases with modified pro regions
GB0922467D0 (en) 2009-04-24 2010-02-03 Danisco Feed supplement
AR076941A1 (es) * 2009-06-11 2011-07-20 Danisco Us Inc Cepa de bacillus para una mayor produccion de proteina
EP2459714A1 (en) 2009-07-31 2012-06-06 Danisco US Inc. Proteases with modified pre-pro regions

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DK2788491T3 (en) 2019-04-01
JP2015500032A (ja) 2015-01-05
CN104053780A (zh) 2014-09-17
EP2788491A1 (en) 2014-10-15
WO2013086219A1 (en) 2013-06-13
US20140329309A1 (en) 2014-11-06
EP2788491B1 (en) 2019-01-23

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