CN103690450A - Composition with skin-whitening and anti-ageing effects, skin care product containing composition as well as preparation method and application of composition - Google Patents
Composition with skin-whitening and anti-ageing effects, skin care product containing composition as well as preparation method and application of composition Download PDFInfo
- Publication number
- CN103690450A CN103690450A CN201310732501.4A CN201310732501A CN103690450A CN 103690450 A CN103690450 A CN 103690450A CN 201310732501 A CN201310732501 A CN 201310732501A CN 103690450 A CN103690450 A CN 103690450A
- Authority
- CN
- China
- Prior art keywords
- compositions
- whitening anti
- aging effect
- water
- skin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Cosmetics (AREA)
Abstract
The invention discloses a composition with skin-whitening and anti-ageing effects, a skin care product containing the composition as well as a preparation method and an application of the composition. The composition is prepared from the following components: 0.2%-10.0% of L-ascorbic acid 2-glucoside, 0.1%-0.2% of superoxide dismutase, 0.1%-0.5% of a grape seed extract, 0.1%-0.5% of a soybean fermentation extract, 10%-30% of propanediol, 0.1%-0.2% of aloe powder and the balance amount of water. An experimental result shows that the composition has the skin-whitening and anti-ageing effects, the method is easy in operation, the production cost is low, and the product stability is high; an evaluation on an in vitro test and a human body skin test proves that the composition has good skin-whitening and anti-ageing effects and is safe and free from side effects; meanwhile, the composition and the skin care product containing the composition, provided by the invention, also have better long-lasting hydrating effects.
Description
Technical field
The present invention relates to a kind of skin care compositions and its application in cosmetics, relate in particular to a kind of have skin care compositions and the preparation method of whitening anti-aging effect, the skin care item that contain this skin care compositions and preparation method.
Background technology
Beautiful and beautiful is the theme that people pursue forever, main embodiment is exactly to pursue the whitening of human body skin and slow down aging, because skin is human body most surface is also maximum organ, but because skin directly contacts with external environment condition, be vulnerable to the stimulation of the factor such as various materials, ray in environment, be easy to come to harm, make easily aging and damage of skin, directly affected the attractive in appearance of human body.
Skin damage hinder major embodiment be in Skin Cell the disappearance of moisture, in the accumulation of metabolic waste, skin the increase of free radical, the loss of nutrient substance etc. in cell.And these problems can be by having the tonic cosmetics of function safeguard and improve by part, so the whitening on market and anti-aging cosmetics are subject to consumers in general's favor very much.More for whitening and antidotal cosmetics and raw material on market, but, comprehensive antidotal skin care item stable in whitening and defying age aspect characteristic are not a lot.
Superoxide dismutase (SOD) is the natural enemy of oxygen-derived free radicals, is the No.1 killer of oxygen-derived free radicals in body, and level in vivo just means old and feeble with dead index directly perceived.SOD can suppress the strong oxidation of oxygen-derived free radicals at skin surface, reach the object of skin protection.SOD makes an addition in cosmetics can effectively prevent that skin is subject to the damage of ionizing radiation, thereby plays sun-proof result; Also skin aging be can effectively prevent, speckle dispelling, crease-resistant effect played; But SOD is poor stability in cosmetics, can not, by timely and effective absorption, so mostly the SOD skin care item in market are concept type product, be difficult to reach the effect in anticipation.The superoxide dismutase that Zhong Xing energy company produces is that microorganism is fermented then through the processing of molecular level, has high temperature resistant and fast light characteristic, biologically active tat 10000-15000U/mg, and comparatively stable, be easily absorbed.There is good whitening and anti-oxidation efficacy.
Semen Vitis viniferae extract is antioxidation, the strongest material of removing free radical ability that current occurring in nature is found, its antioxidant activity is V
e50 times, V
c20 times, it can effectively remove unnecessary free radical in human body, has the effect of superpower slow down aging and enhancing immunity.Fermented soybean extract is rich in plant soybean isoflavone, there is similar human body estrogen action, pass through antioxidation, restraint of tyrosinase activity etc., show extremely strong whitening effect, also have and promote collagen protein and the synthetic effect of hyaluronic acid, make that skin is fine and smooth, pure white, high resilience and gloss.
The present invention, according to the function of each material and feature, optimizes proportioning and extraction by multicomponent, obtains whitening anti-aging compositions, and is prepared into skin care item.
Summary of the invention
The object of this invention is to provide a kind of compositions with whitening anti-aging effect.
Second object of the present invention is to provide a kind of preparation method with the compositions of whitening anti-aging effect.
The 3rd object of the present invention is to provide a kind of skin care item that contain the compositions with whitening anti-aging effect.
The 4th object of the present invention is to provide a kind of preparation method of the skin care item that contain the compositions with whitening anti-aging effect.
The 5th object of the present invention is to provide a kind of purposes with the compositions of whitening anti-aging effect.
The 6th object of the present invention is to provide a kind of purposes of the skin care item that contain the compositions with whitening anti-aging effect.
A kind of compositions with whitening anti-aging effect, to make by following component: AA 2G 0.2%-10.0%, superoxide dismutase 0.1%-0.2%, Semen Vitis viniferae extract 0.1%-0.5%, fermented soybean extract 0.1%-0.5%, propylene glycol 10%-30%, Aloe powder 0.1%-0.2%, surplus is deionized water.
Described superoxide dismutase is that microorganism fermentation prepares, and biological activity is 10000-15000U/mg.
A preparation method with the compositions of whitening anti-aging effect, comprises the steps:
1) taking by weight following component is raw material: AA 2G 0.2%-10.0%, superoxide dismutase 0.1%-0.2%, Semen Vitis viniferae extract 0.1%-0.5%, fermented soybean extract 0.1%-0.5%, propylene glycol 10%-30%, Aloe powder 0.1%-0.2%, surplus is deionized water;
2) by AA 2G, superoxide dismutase, Semen Vitis viniferae extract and fermented soybean extract, join in the deionized water of raw materials quality 50%-60% described in described step 1), heating in water bath is to 60-70 ℃, heating 10-20min;
3) Aloe powder is spilled in propylene glycol, is heated to 60-70 ℃, stir 5-10min;
4) mixture step 3) being obtained joins step 2) in the liquid that obtains, add deionized water to 100%, stir; Be heated to 85-90 ℃, insulation 25-30min; Psychrolusia is cooled to below 45 ℃, obtains a kind of compositions with whitening anti-aging effect.
Described superoxide dismutase is that microorganism fermentation prepares, and biological activity is 10000-15000U/mg.
A kind of skin care item that contain the compositions with whitening anti-aging effect, it is to make by following component: disodiumedetate 0.02%, glycerol 5.0%-10.0%, 1,3-butanediol 4.0%-8.0%, glycyrrhizic acid dipotassium 4.0%-8.0%, xanthan gum 0.1%-0.3%, water solublity Jojoba oil 0.4%-0.8%, water-solubleazone 0.1%-0.2%, D-panthenol 0.1%-0.2%, has the compositions 20.0%-40.0% of whitening anti-aging effect, carbomer 0.5%, triethanolamine 0.5%-1.0%, essence 0.01%, PHG0.5%, surplus is deionized water.
A preparation method for the skin care item that contain the compositions with whitening anti-aging effect, comprises the steps:
1) taking by weight following component is raw material:
Disodiumedetate 0.02%, glycerol 5.0%-10.0%, 1,3-butanediol 4.0%-8.0%, glycyrrhizic acid dipotassium 4.0%-8.0%, xanthan gum 0.1%-0.3%, water solublity Jojoba oil 0.4%-0.8%, water-solubleazone 0.1%-0.2%, D-panthenol 0.1%-0.2%, the compositions 20.0%-40.0% with whitening anti-aging effect, carbomer 0.5%, triethanolamine 0.5%-1.0%, essence 0.01%, PHG0.5%, surplus is deionized water;
2) carbomer is spilled in the deionized water of described step 1) raw materials quality 30%, standing 2-4h, makes the carbomer swelling that fully absorbs water; Obtain mixture a, be heated to 60-70 ℃;
3) disodiumedetate, glycyrrhizic acid dipotassium, glycerol, 1,3 butylene glycol are joined in the deionized water of described step 1) raw materials quality 10% successively, stir 10min-20min; Be heated to 60-70 ℃, add successively water solublity Jojoba oil, water-solubleazone, D-panthenol, 400rpm-500rpm stirs 10min-20min and under 500rpm-600rpm stirring condition, is spilled into xanthan gum again, obtains mixture b;
4) mixture a, b are mixed with the compositions with whitening anti-aging effect, supplement deionized water to 98.49-98.99%; Under water-bath 70-80 ℃ condition, 300rpm-400rpm stirs 10min-20min, dropwise adds triethanolamine, is gel to solution; Under 80-85 ℃ of condition, be incubated 20min-30min;
5) cold water is cooled to below 40 ℃, adds essence and antiseptic PHG, obtains a kind of skin care item that contain the compositions with whitening anti-aging effect.
The compositions with whitening anti-aging effect has the purposes of whitening anti-aging skin care item in preparation.
The skin care item that contain the compositions with whitening anti-aging effect purposes.
Experimental result shows, the skin care item that have the compositions of whitening anti-aging effect and contain the compositions with whitening anti-aging effect of the present invention have the effect of whitening anti-aging, and for making the substrate of skin care item without the effect of whitening anti-aging, illustrate that the present composition is suitable as cosmetic industry composition and adds use.
The preparation method of a kind of compositions with whitening anti-aging effect of the present invention is simple to operate, and production cost is low, and product stability is good; The compositions with whitening anti-aging effect provided by the invention is the bright liquid of colourless or lurid clarification; The skin care item that have the compositions of whitening anti-aging effect and contain said composition provided by the invention confirm to have good whitening and senile-resistant efficacy through in vitro tests and human body skin test evaluation, and safety has no side effect; Meanwhile, the skin care item that have the compositions of whitening anti-aging effect and contain said composition provided by the invention also have good long-acting moistening effect.
Accompanying drawing explanation
Fig. 1 is the compositions with whitening anti-aging effect of the embodiment of the present invention 4,5, the 6 preparation maximum inhibition curve to TYR enzyme;
Fig. 2 is the clearance rate numerical value figure of the compositions with whitening anti-aging effect prepared of the method for the embodiment of the present invention 4 to DPPH free radical;
Fig. 3 is the human body skin roughness Assessment of Changes figure of a kind of skin care item that contain the compositions with whitening anti-aging effect prepared by the method for the embodiment of the present invention 10;
Fig. 4 is the human body skin Flexible change evaluation map of a kind of skin care item that contain the compositions with whitening anti-aging effect prepared by the method for the embodiment of the present invention 10.
The specific embodiment
The test method of using in following embodiment if no special instructions, is conventional method.
In following embodiment, material used, reagent etc., if no special instructions, all can obtain from commercial channels.The source of following raw material is only for the present invention being described, not limiting the invention, with the same or analogous product of product specification in following source can be for the present invention.
Superoxide dismutase is that microorganism fermentation prepares (in the emerging energy (Tianjin) company limited produce), and biological activity is 10000-15000U/mg.
Semen Vitis viniferae extract (being purchased from Yuan Sen bio tech ltd, Xi'an).
Fermented soybean extract (in the emerging energy (Tianjin) company limited produce).
Aloe powder (the international holding company of U.S.'s thyrite produces)
PHG is compound preservative (be 65% phenoxyethanol by mass ratio, 35% ethohexadiol is composite to be formed).PHG is
pHG(composition: phenoxyethanol PHENOXYETHANOL, 1,2 ethohexadiol CAPRYLYL GLYCOL), for THOR company produces phenoxyethanol class antiseptic, purchased from Shanghai Qian Yi chemicals company limited.
A kind of compositions with whitening anti-aging effect, by following component, make: AA 2G 5g, superoxide dismutase 0.1g, Semen Vitis viniferae extract 0.3g, fermented soybean extract 0.3g, propylene glycol 20g, Aloe powder 0.1g, deionized water 74.2g, superoxide dismutase is wherein that microorganism fermentation prepares, biological activity is 13000U/mg.
A compositions with whitening anti-aging effect, makes by following component: AA 2G 0.2g, superoxide dismutase 0.2g, Semen Vitis viniferae extract 0.1g, fermented soybean extract 0.5g, propylene glycol 10g, Aloe powder 0.2g, deionized water 88.8g.Superoxide dismutase is wherein that microorganism fermentation prepares, and biological activity is 10000U/mg.
A compositions with whitening anti-aging effect, makes by following component: AA 2G 10.0g, superoxide dismutase 0.1g, Semen Vitis viniferae extract 0.5g, fermented soybean extract 0.1g, propylene glycol 30g, Aloe powder 0.1g, deionized water 59.2g.Superoxide dismutase is wherein that microorganism fermentation prepares, and biological activity is 15000U/mg.
A preparation method with the compositions of whitening anti-aging effect, comprises the steps:
1) taking by weight following component is raw material: AA 2G 5g, superoxide dismutase 0.1g, Semen Vitis viniferae extract 0.3g, fermented soybean extract 0.3g, propylene glycol 20g, Aloe powder 0.1g, deionized water 74.2g, superoxide dismutase is wherein that microorganism fermentation prepares, and biological activity is 13000U/mg;
2), by AA 2G, superoxide dismutase, Semen Vitis viniferae extract and fermented soybean extract, add in 55g deionized water heating in water bath to 65 ℃, heating 15min;
3) Aloe powder is spilled in propylene glycol, is heated to 65 ℃, stir 8min;
4) mixture step 3) being obtained joins step 2) in the liquid that obtains, add deionized water to 100g, stir; Be heated to 88 ℃, insulation 28min; Psychrolusia is cooled to below 45 ℃, obtains a kind of compositions with whitening anti-aging effect.
Corrupt in order to prevent above-mentioned composition, 0.5g antiseptic phenoxyethanol (Phenoxyethanol) can also be joined in the compositions of above-mentioned 100g.
Above-mentioned composition will be preserved under room temperature lucifuge condition, standby.
A preparation method with the compositions of whitening anti-aging effect, comprises the steps:
1) taking by weight following component is raw material: AA 2G 0.2g, superoxide dismutase 0.2g, Semen Vitis viniferae extract 0.1g, fermented soybean extract 0.5g, propylene glycol 10g, Aloe powder 0.2g, deionized water 88.8g.Superoxide dismutase is wherein that microorganism fermentation prepares, and biological activity is 10000U/mg;
2), by AA 2G, superoxide dismutase, Semen Vitis viniferae extract and fermented soybean extract, add in 60g deionized water heating in water bath to 70 ℃, heating 10min;
3) Aloe powder is spilled in propylene glycol, is heated to 70 ℃, stir 5min;
4) mixture step 3) being obtained joins step 2) in the liquid that obtains, add deionized water to 100g, stir; Be heated to 90 ℃, insulation 25min; Psychrolusia is cooled to below 45 ℃, obtains a kind of compositions with whitening anti-aging effect.
Corrupt in order to prevent above-mentioned composition, 0.5g antiseptic phenoxyethanol (Phenoxyethanol) can also be joined in the compositions of above-mentioned 100g.
Above-mentioned composition will be preserved under room temperature lucifuge condition, standby.
Embodiment 6
A preparation method with the compositions of whitening anti-aging effect, comprises the steps:
1) taking by weight following component is raw material: AA 2G 10.0g, superoxide dismutase 0.1g, Semen Vitis viniferae extract 0.5g, fermented soybean extract 0.1g, propylene glycol 30g, Aloe powder 0.1g, deionized water 59.2g.Superoxide dismutase is wherein that microorganism fermentation prepares, and biological activity is 15000U/mg;
2), by AA 2G, superoxide dismutase, Semen Vitis viniferae extract and fermented soybean extract, add in 50g deionized water heating in water bath to 60 ℃, heating 20min;
3) Aloe powder is spilled in propylene glycol, is heated to 60 ℃, stir 10min;
4) mixture step 3) being obtained joins step 2) in the liquid that obtains, add deionized water to 100g, stir; Be heated to 85 ℃, insulation 30min; Psychrolusia is cooled to below 45 ℃, obtains a kind of compositions with whitening anti-aging effect.
Corrupt in order to prevent above-mentioned composition, 0.5g antiseptic phenoxyethanol (Phenoxyethanol) can also be joined in the compositions of above-mentioned 100g.
Above-mentioned composition will be preserved under room temperature lucifuge condition, standby.
Embodiment 7
Skin care item for the compositions with whitening anti-aging effect that contains embodiment 4 preparations, by following component, make:
Disodiumedetate 0.02g, glycerol 8.0g, 1,3-butanediol 6.0g, glycyrrhizic acid dipotassium 6.0g, xanthan gum 0.2g, water solublity Jojoba oil 0.6g, water-solubleazone 0.2g, D-panthenol 0.2g, the compositions 30.0g with whitening anti-aging effect of embodiment 4 preparations, carbomer 0.5g, triethanolamine 0.8g, essence 0.01g, PHG0.5g, deionized water 46.97g.
Embodiment 8
Skin care item for the compositions with whitening anti-aging effect that contains embodiment 5 preparations, by following component, make:
Disodiumedetate 0.02g, glycerol 5.0g, 1,3-butanediol 8.0g, glycyrrhizic acid dipotassium 4.0g, xanthan gum 0.3g, water solublity Jojoba oil 0.4g, water-solubleazone 0.2g, D-panthenol 0.1g, the compositions 40.0g with whitening anti-aging effect of embodiment 5 preparations, carbomer 0.5g, triethanolamine 0.5g, essence 0.01g, PHG0.5g, deionized water 40.47g.
Embodiment 9
Skin care item for the compositions with whitening anti-aging effect that contains embodiment 6 preparations, by following component, make:
Disodiumedetate 0.02g, glycerol 10.0g, 1,3-butanediol 4.0g, glycyrrhizic acid dipotassium 8.0g, xanthan gum 0.1g, water solublity Jojoba oil 0.8g, water-solubleazone 0.1g, D-panthenol 0.2g, the compositions 20.0g with whitening anti-aging effect of embodiment 6 preparations, carbomer 0.5g, triethanolamine 1.0g, essence 0.01g, PHG0.5g, deionized water 45.23g.
A preparation method for the skin care item that contain the compositions with whitening anti-aging effect, comprises the steps:
1) taking by weight following component is raw material:
Disodiumedetate 0.02g, glycerol 8.0g, 1,3-butanediol 6.0g, glycyrrhizic acid dipotassium 6.0g, xanthan gum 0.2g, water solublity Jojoba oil 0.6g, water-solubleazone 0.2g, D-panthenol 0.2g, the compositions 30.0g with whitening anti-aging effect of embodiment 4 preparations, carbomer 0.5g, triethanolamine 0.8g, essence 0.01g, PHG0.5g, deionized water 46.97g;
2) carbomer is spilled in the deionized water of 30g, standing 3h, makes the carbomer swelling that fully absorbs water; Obtain mixture a, be heated to 65 ℃;
3) disodiumedetate, glycyrrhizic acid dipotassium, glycerol, 1,3 butylene glycol are joined in 10g deionized water successively, stir 15min; Be heated to 65 ℃, add successively water solublity Jojoba oil, water-solubleazone, D-panthenol, 500rpm stirs 15min and under 600rpm stirring condition, is spilled into xanthan gum again, obtains mixture b;
4) mixture a, b are mixed with the compositions with whitening anti-aging effect, supplement deionized water to 98.69g; Under 75 ℃ of conditions of water-bath, 400rpm stirs 15min, dropwise adds triethanolamine, is gel to solution; Under 83 ℃ of conditions, be incubated 25min;
5) cold water is cooled to below 40 ℃, adds essence and antiseptic PHG, obtains a kind of skin care item that contain the compositions with whitening anti-aging effect.
Embodiment 11
A preparation method for the skin care item that contain the compositions with whitening anti-aging effect, comprises the steps:
1) taking by weight following component is raw material:
Disodiumedetate 0.02g, glycerol 5.0g, 1,3-butanediol 8.0g, glycyrrhizic acid dipotassium 4.0g, xanthan gum 0.3g, water solublity Jojoba oil 0.4g, water-solubleazone 0.2g, D-panthenol 0.1g, the compositions 40.0g with whitening anti-aging effect of embodiment 5 preparations, carbomer 0.5g, triethanolamine 0.5g, essence 0.01g, PHG0.5g, deionized water 40.47g
2) carbomer is spilled in the deionized water of 30g, standing 2h, makes the carbomer swelling that fully absorbs water; Obtain mixture a, be heated to 70 ℃;
3) disodiumedetate, glycyrrhizic acid dipotassium, glycerol, 1,3 butylene glycol are joined in 10g deionized water successively, stir 10min; Be heated to 70 ℃, add successively water solublity Jojoba oil, water-solubleazone, D-panthenol, 400rpm stirs 20min and under 500rpm stirring condition, is spilled into xanthan gum again, obtains mixture b;
4) mixture a, b are mixed with the compositions with whitening anti-aging effect, supplement deionized water to 98.99g; Under 80 ℃ of conditions of water-bath, 300rpm stirs 10min, dropwise adds triethanolamine, is gel to solution; Under 85 ℃ of conditions, be incubated 20min;
5) cold water is cooled to below 40 ℃, adds essence and antiseptic PHG, obtains a kind of skin care item that contain the compositions with whitening anti-aging effect.
Embodiment 12
A preparation method for the skin care item that contain the compositions with whitening anti-aging effect, comprises the steps:
1) taking by weight following component is raw material:
Disodiumedetate 0.02g, glycerol 10.0g, 1,3-butanediol 4.0g, glycyrrhizic acid dipotassium 8.0g, xanthan gum 0.1g, water solublity Jojoba oil 0.8g, water-solubleazone 0.1g, D-panthenol 0.2g, the compositions 20.0g with whitening anti-aging effect of embodiment 6 preparations, carbomer 0.5g, triethanolamine 1.0g, essence 0.01g, PHG0.5g, deionized water 45.23g;
2) carbomer is spilled in the deionized water of 30g, standing 4h, makes the carbomer swelling that fully absorbs water; Obtain mixture a, be heated to 60 ℃;
3) disodiumedetate, glycyrrhizic acid dipotassium, glycerol, 1,3 butylene glycol are joined in 10g deionized water successively, stir 20min; Be heated to 60 ℃, add successively water solublity Jojoba oil, water-solubleazone, D-panthenol, 500rpm stirs 10min and under 600rpm stirring condition, is spilled into xanthan gum again, obtains mixture b;
4) mixture a, b are mixed with the compositions with whitening anti-aging effect, supplement deionized water to 98.49g; Under 70 ℃ of conditions of water-bath, 400rpm stirs 20min, dropwise adds triethanolamine, is gel to solution; Under 80 ℃ of conditions, be incubated 30min;
5) cold water is cooled to below 40 ℃, adds essence and antiseptic PHG, obtains a kind of skin care item that contain the compositions with whitening anti-aging effect.
Embodiment 13
The white-skinned face function test of the compositions with whitening anti-aging effect of embodiment 4,5,6 preparations: TYR inhibition of enzyme activity test.
Tryrosinase is rate-limiting enzyme main in melanin forming process, and the activity size of this enzyme is determining the quantity that melanin forms, and tyrosinase activity is high, generates and increases with its product melanin of tyrosine effect, finds expression in and on skin of face, produces various black speck.Therefore, restraint of tyrosinase activity is effectively to reduce a kind of approach that melanin generates.Can there is catalytic reaction in TYR enzyme and its substrate TYR, when be added with the reagent of TYR inhibition of enzyme activity effect in reaction system after, to catalytic reaction, can produce inhibitory action, by mensuration, be added with the variation of 475nm place, the reagent front and back absorbance of TYR inhibition of enzyme activity effect, evaluate the suppression ratio of this reagent that has the effect of TYR inhibition of enzyme activity to TYR enzymatic activity.
Concrete process of the test is as follows:
1) take 17.91g disodium hydrogen phosphate and be dissolved in 500mL distilled water, obtain solution a; Take 7.8g sodium dihydrogen phosphate dihydrate and be dissolved in 500mL distilled water, obtain solution b; 92.6mL solution a and 107.4mL solution b are mixed, obtain 200mL pH and be 6.8 PBS buffer solution;
2) take 0.05g TYR, first use the dissolving with hydrochloric acid of a small amount of 0.1mol/L, after it dissolves, with the PBS buffer that the pH of above-mentioned preparation is 6.8, be settled to 100mL;
In process of the test, in each group, the addition of each material is as shown in table 1.
The addition of the compositions that table 1 has a whitening anti-aging effect to each material in the active suppression test of TYR enzyme
Note: C
1group and T
1group all adds 0.5mL tryrosinase, and enzyme is lived as 100U/mL.
3) C
0after organizing each solution and preparing and shake up, heating in water bath 10min in 37 ℃ of water-baths, returns to zero under wavelength 475nm.C
1after group solution prepares and shakes up, after 37 ℃ of water-bath 10min, adding the work of 0.5ml enzyme is the tryrosinase of 100U/mL, continues water-bath 10 minutes, measures absorbance;
4) T
0after organizing each solution and preparing and shake up, heating in water bath 10min in 37 ℃ of water-baths, returns to zero under wavelength 475nm.T
1after group solution prepares and shakes up, after 37 ℃ of water-bath 10min, adding the work of 0.5ml enzyme is the tryrosinase of 100U/mL, continues water-bath 10 minutes, measures absorbance;
5) calculate the maximum inhibition T(% of tryrosinase), T(%)=(C
1-T
1)/C
1* 100%.
Result of the test as shown in Figure 1. Test group 1,2,3 is corresponding embodiment 4,5,6 respectively, and when having, the compositions of whitening anti-aging effect is obvious to tyrosinase activity inhibition, and suppression ratio increases with the increase of composition concentration; Wherein, when composition concentration is 10%, tyrosinase activity suppression ratio meansigma methods is 59.1%, and peak reaches 64.8%; When concentration is 30% concentration, suppression ratio reaches 90.5%, and when concentration is 40% concentration, suppression ratio reaches 92.1%.Result shows that whitening anti-aging compositions is obvious to tyrosinase activity inhibition, and it has significant whitening effect.
Embodiment 14
The white-skinned face function test of a kind of skin care item that contain the compositions with whitening anti-aging effect of embodiment 12 preparations
The test of human body white-skinned face function, adopts human test's mode, measures melanin content and the brightness of skin with the dark red pigment tester of skin and skin colourity test probe.
Choose 30 volunteers, in 20-40 year, men and women half and half, and tested position can not be used any product for first 2～3 days; With dry medicated napkin by wiped clean inside both hands forearm and be 4 * 4cm
2pilot region is measured labelling, measures skin brightness and the melanin content of tested skin with skin colourity test probe+MPA9 tester and the dark red pigment tester of skin; With latex finger cot, will test sample and blank group (blank group for not containing the substrate of whitening anti-aging compositions) by 2.0 ± 0.1mg sample/cm
2consumption spread upon respectively on the arm of left and right, smear rear skin brightness and the melanin content of measuring respectively 1st～4 weeks each regions.
(1) mutation analysis of skin brightness
Skin brightness variation was reflected in test period, investigated Experimental Area skin brightness L-value change with time.Its value is larger, and skin brightness is larger, otherwise skin brightness is lower.L-value (T before each time point L-value (Tn)-use product after skin brightness difference (Δ T)=use product
0).
The difference of the tested position of table 2 skin brightness L-value and initial value
During test 4 weeks, the tested position of volunteer skin is smeared after blank group, and skin brightness numerical value does not significantly change, and illustrates that the substrate of smearing does not have the ability that improves skin brightness.And at test period, the difference of the tested position of sample sets skin brightness L-value and initial value be always on the occasion of, and skin brightness difference raises gradually along with the prolongation of testing time, shows that the skin care item that contain whitening anti-aging compositions have obvious whitening effect.
(2) melanin content mutation analysis
Dermal melanin changes of contents was reflected in test period, pilot region dermal melanin content change with time.Its value is larger, and dermal melanin content is larger, otherwise, dermal melanin content.Melanin content (T0) before each time point melanin content (Tn)-use product after melanin content difference (Δ T)=use product.
The difference of the tested position of table 3 dermal melanin content and initial value
During test 4 weeks, the tested position of volunteer skin is smeared after blank group, the melanin content containing in skin be on the occasion of, and numerical value is without significant change, illustrates that the blank group substrate of smearing do not eliminate or shift melanic ability.And at test period, the melanin content of the tested position of sample sets skin and the difference of initial value are negative value always, and interpret sample group is that a kind of skin care item that contain the compositions with whitening anti-aging effect of embodiment 12 preparations have the ability that reduces or shift melanin content; And melanin reduces gradually containing changing prolongation in time, shows that sample sets has obvious whitening effect.
The skin care item that contain the compositions with whitening anti-aging effect that experiment showed, embodiment 10 and embodiment 11 preparations have the effect similar to the skin care item of embodiment 12 preparations.
Embodiment 15
The senile-resistant efficacy test of the compositions with whitening anti-aging effect prepared by the method for embodiment 4: remove the experiment of DPPH free radical.
DPPH is a kind of very stable free radical, if compositions can be removed, represents that compositions has the effect of the valid density that reduces hydroxy radical or oxyradical.DPPH has a characteristic absorption peak at 517nm place, when running into free radical scavenger, DPPH diminishes at the absorbance of maximum absorption wave strong point.Therefore, can evaluate its removing effect to DPPH by measuring the variation of absorbance.
Accurately take 20mg DPPH, with dehydrated alcohol, be settled in 250ml volumetric flask, obtaining concentration is the DPPH solution of 20mmol/L, compositions is diluted to the test fluid of variable concentrations.Get respectively test fluid and the 2ml20mmol/LDPPH of 2ml variable concentrations, mix rear reaction 30min, measure the absorbance under 517nm wavelength, control solvent replaces with dehydrated alcohol, is calculated as follows suppression ratio.Suppression ratio (%)=[1-(Ai-Aj/Ac] * 100; Wherein, Ai is the absorbance of the mixed liquor that forms of the test fluid of 2ml DPPH solution and 2ml variable concentrations; Aj is the test fluid of 2ml variable concentrations and the absorbance of the mixed liquor that 2ml dehydrated alcohol forms; Ac is the absorbance of the mixed liquor of 2ml DPPH solution and 2ml dehydrated alcohol composition.
Compositions is comparatively obvious to DPPH scavenging action, as shown in Figure 2, the composition solution of low concentration remove ability a little less than, but along with the increase of concentration, DPPH free radical scavenging activity increases thereupon.When composition concentration is 20%, clearance rate reaches 64%; When concentration is 40%, clearance rate reaches 80%; Result shows, when composition concentration is during at 20%-40%, whitening anti-aging composition removing DPPH free radical effect is remarkable, has obvious antidotal effect.
Embodiment 16
A kind of skin care item skin elasticity testing experiment that contains the compositions with whitening anti-aging effect prepared by the method for embodiment 10, detailed process is as follows:
Using German Khazaka(CK)+skin elasticity tester the MPA580 of Courage company and Reviscometer RV600 probe is to 30 volunteers, and 30-50 year, men and women half and half, and tested position face is tested, detection of skin elasticity and roughness parameter.
Smearing in 4 weeks, detecting once weekly, and skin elasticity and roughness are graded, its empty group is not contain the substrate of whitening anti-aging compositions.
As Fig. 3 and Fig. 4, result shows, in 4 weeks that detect, use the skin mean roughness parameter of blank sample not have significant change, test group personnel's skin is along with the prolongation of service time, and skin coarseness is on a declining curve, and in the time of the 4th week, the decline of skin mean roughness reaches 36%; Subject's face is observed to detection, and skin surface microgroove attenuates little, and smoothness strengthens, and shows that skin care item of the present invention can significantly improve the roughness of human body skin.In 4 weeks that detect, use the skin mean roughness parameter of blank sample to remain basically stable, skin elasticity is increase trend with the prolongation of service time, in the time of the 4th week, coefficient of skin elasticity value is 7.9, than the 0th week numerical value, has increased by 21%, shows that subject's skin elasticity is significantly improved.
Experiment shows, the skin care item that a kind of skin care item that contain the compositions with whitening anti-aging effect prepared by the method for embodiment 11,12 are prepared to the method for embodiment 10 have similar effect.
The compositions of whitening anti-aging effect can also method routinely be prepared emulsion, essence, essence or cream frost etc.
Claims (8)
1. a compositions with whitening anti-aging effect, it is characterized in that making by following component: AA 2G 0.2%-10.0%, superoxide dismutase 0.1%-0.2%, Semen Vitis viniferae extract 0.1%-0.5%, fermented soybean extract 0.1%-0.5%, propylene glycol 10%-30%, Aloe powder 0.1%-0.2%, surplus is deionized water.
2. a kind of compositions with whitening anti-aging effect according to claim 1, is characterized in that, described superoxide dismutase is that microorganism fermentation prepares, and biological activity is 10000-15000U/mg.
3. a kind of preparation method with the compositions of whitening anti-aging effect of claim 1, is characterized in that comprising the steps:
1) taking by weight following component is raw material: AA 2G 0.2%-10.0%, superoxide dismutase 0.1%-0.2%, Semen Vitis viniferae extract 0.1%-0.5%, fermented soybean extract 0.1%-0.5%, propylene glycol 10%-30%, Aloe powder 0.1%-0.2%, surplus is deionized water;
2) by AA 2G, superoxide dismutase, Semen Vitis viniferae extract and fermented soybean extract, join in the deionized water of raw materials quality 50%-60% described in described step 1), heating in water bath is to 60-70 ℃, heating 10-20min;
3) Aloe powder is spilled in propylene glycol, is heated to 60-70 ℃, stir 5-10min;
4) mixture step 3) being obtained joins step 2) in the liquid that obtains, add deionized water to 100%, stir; Be heated to 85-90 ℃, insulation 25-30min; Psychrolusia is cooled to below 45 ℃, obtains a kind of compositions with whitening anti-aging effect.
4. a kind of preparation method with the compositions of whitening anti-aging effect claimed in claim 3, is characterized in that superoxide dismutase is that microorganism fermentation prepares, and biological activity is 10000-15000U/mg.
5. the skin care item of a compositions with whitening anti-aging effect that contains claim 1 or 2, it is characterized in that making by following component: disodiumedetate 0.02%, glycerol 5.0%-10.0%, 1, 3-butanediol 4.0%-8.0%, glycyrrhizic acid dipotassium 4.0%-8.0%, xanthan gum 0.1%-0.3%, water solublity Jojoba oil 0.4%-0.8%, water-solubleazone 0.1%-0.2%, D-panthenol 0.1%-0.2%, the compositions 20.0%-40.0% with whitening anti-aging effect, carbomer 0.5%, triethanolamine 0.5%-1.0%, essence 0.01%, PHG0.5%, surplus is deionized water.
6. the preparation method of claim 5 skin care item that contain the compositions with whitening anti-aging effect, is characterized in that comprising the steps:
1) taking by weight following component is raw material:
Disodiumedetate 0.02%, glycerol 5.0%-10.0%, 1,3-butanediol 4.0%-8.0%, glycyrrhizic acid dipotassium 4.0%-8.0%, xanthan gum 0.1%-0.3%, water solublity Jojoba oil 0.4%-0.8%, water-solubleazone 0.1%-0.2%, D-panthenol 0.1%-0.2%, the compositions 20.0%-40.0% with whitening anti-aging effect, carbomer 0.5%, triethanolamine 0.5%-1.0%, essence 0.01%, PHG0.5%, surplus is deionized water;
2) carbomer is spilled in the deionized water of described step 1) raw materials quality 30%, standing 2-4h, makes the carbomer swelling that fully absorbs water; Obtain mixture a, be heated to 60-70 ℃;
3) disodiumedetate, glycyrrhizic acid dipotassium, glycerol, 1,3 butylene glycol are joined in the deionized water of described step 1) raw materials quality 10% successively, stir 10min-20min; Be heated to 60-70 ℃, add successively water solublity Jojoba oil, water-solubleazone, D-panthenol, 400rpm-500rpm stirs 10min-20min and under 500rpm-600rpm stirring condition, is spilled into xanthan gum again, obtains mixture b;
4) mixture a, b are mixed with the compositions with whitening anti-aging effect, supplement deionized water to 98.49-98.99%; Under water-bath 70-80 ℃ condition, 300rpm-400rpm stirs 10min-20min, dropwise adds triethanolamine, is gel to solution; Under 80-85 ℃ of condition, be incubated 20min-30min;
5) cold water is cooled to below 40 ℃, adds essence and antiseptic PHG, obtains a kind of skin care item that contain the compositions with whitening anti-aging effect.
7. according to the compositions with whitening anti-aging effect of claim 1 or 2, in preparation, there is the purposes of whitening anti-aging skin care item.
8. according to the purposes of the skin care item of a kind of compositions with whitening anti-aging effect that contains claim 1 or 2 of claim 5.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310732501.4A CN103690450B (en) | 2013-12-20 | 2013-12-20 | There is the compositions of whitening anti-aging effect, skin care item and preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310732501.4A CN103690450B (en) | 2013-12-20 | 2013-12-20 | There is the compositions of whitening anti-aging effect, skin care item and preparation method and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103690450A true CN103690450A (en) | 2014-04-02 |
| CN103690450B CN103690450B (en) | 2016-04-20 |
Family
ID=50352226
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310732501.4A Expired - Fee Related CN103690450B (en) | 2013-12-20 | 2013-12-20 | There is the compositions of whitening anti-aging effect, skin care item and preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103690450B (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104738635A (en) * | 2015-04-13 | 2015-07-01 | 广州赛莱拉干细胞科技股份有限公司 | Antioxidative composition and preparation method thereof |
| CN106265372A (en) * | 2016-09-30 | 2017-01-04 | 广东丸美生物技术股份有限公司 | A kind of skin matrix with senile-resistant efficacy and preparation method and application |
| CN108379170A (en) * | 2018-05-08 | 2018-08-10 | 广州奥蓓斯化妆品有限公司 | A kind of composite skin care product of skin whitening |
| CN108514519A (en) * | 2018-05-11 | 2018-09-11 | 珠海伊斯佳科技股份有限公司 | A kind of skin care compositions and methods and its application method of whitening and anti-aging |
| CN110960441A (en) * | 2019-12-12 | 2020-04-07 | 宁波久瑞生物工程有限公司 | SOD composition with stable activity and preparation process and application thereof |
| CN112891270A (en) * | 2021-01-29 | 2021-06-04 | 洪怡 | Cosmetic composition and preparation method and application thereof |
| CN113952250A (en) * | 2021-11-02 | 2022-01-21 | 上海忱扬生物科技有限公司 | Stable anti-photoaging composition and preparation method thereof |
| CN115040446A (en) * | 2022-06-24 | 2022-09-13 | 曾会明 | SOD plant extract skin care composition, skin care product and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999033439A1 (en) * | 1997-12-24 | 1999-07-08 | Shaklee Corporation | Composition with high efficiency skin protection from damaging effects of ultraviolet light |
| CN101184469A (en) * | 2005-04-28 | 2008-05-21 | 独立行政法人科学技术振兴机构 | Skin regeneration promoter |
| KR20100137219A (en) * | 2009-06-22 | 2010-12-30 | (주)아모레퍼시픽 | Mask pack using korean paper layered red ginseng and the preparation thereof |
| CN101953772A (en) * | 2010-09-21 | 2011-01-26 | 广州舒泰生物技术有限公司 | Whitening sunscreen cosmetic and preparation method and application thereof |
-
2013
- 2013-12-20 CN CN201310732501.4A patent/CN103690450B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999033439A1 (en) * | 1997-12-24 | 1999-07-08 | Shaklee Corporation | Composition with high efficiency skin protection from damaging effects of ultraviolet light |
| CN101184469A (en) * | 2005-04-28 | 2008-05-21 | 独立行政法人科学技术振兴机构 | Skin regeneration promoter |
| KR20100137219A (en) * | 2009-06-22 | 2010-12-30 | (주)아모레퍼시픽 | Mask pack using korean paper layered red ginseng and the preparation thereof |
| CN101953772A (en) * | 2010-09-21 | 2011-01-26 | 广州舒泰生物技术有限公司 | Whitening sunscreen cosmetic and preparation method and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 兰兰等: "研发动态", 《中国化妆品》 * |
| 徐先玲等: "《美容化妆指南》", 31 December 2005, 中国戏剧出版社 * |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104738635A (en) * | 2015-04-13 | 2015-07-01 | 广州赛莱拉干细胞科技股份有限公司 | Antioxidative composition and preparation method thereof |
| CN106265372A (en) * | 2016-09-30 | 2017-01-04 | 广东丸美生物技术股份有限公司 | A kind of skin matrix with senile-resistant efficacy and preparation method and application |
| CN106265372B (en) * | 2016-09-30 | 2021-05-11 | 广东丸美生物技术股份有限公司 | Skin care matrix with anti-aging effect and preparation method and application thereof |
| CN108379170A (en) * | 2018-05-08 | 2018-08-10 | 广州奥蓓斯化妆品有限公司 | A kind of composite skin care product of skin whitening |
| CN108514519A (en) * | 2018-05-11 | 2018-09-11 | 珠海伊斯佳科技股份有限公司 | A kind of skin care compositions and methods and its application method of whitening and anti-aging |
| CN110960441A (en) * | 2019-12-12 | 2020-04-07 | 宁波久瑞生物工程有限公司 | SOD composition with stable activity and preparation process and application thereof |
| CN110960441B (en) * | 2019-12-12 | 2022-09-13 | 宁波久瑞生物工程有限公司 | SOD composition with stable activity and preparation process and application thereof |
| CN112891270A (en) * | 2021-01-29 | 2021-06-04 | 洪怡 | Cosmetic composition and preparation method and application thereof |
| CN113952250A (en) * | 2021-11-02 | 2022-01-21 | 上海忱扬生物科技有限公司 | Stable anti-photoaging composition and preparation method thereof |
| CN113952250B (en) * | 2021-11-02 | 2023-11-21 | 上海忱扬生物科技有限公司 | Stable photo-aging resistant composition and preparation method thereof |
| CN115040446A (en) * | 2022-06-24 | 2022-09-13 | 曾会明 | SOD plant extract skin care composition, skin care product and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103690450B (en) | 2016-04-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103690450B (en) | There is the compositions of whitening anti-aging effect, skin care item and preparation method and application | |
| CN102366397B (en) | Skin-whitening chloasma-reducing skin-brightening composition, skin care product therewith, and preparation method thereof | |
| CN113616566B (en) | Skin care essence for tightening skin and preparation method thereof | |
| CN112494406A (en) | Whitening and freckle-removing cosmetic and preparation method thereof | |
| KR20140012054A (en) | Multi-purpose cosmetic compositions | |
| SG190342A1 (en) | Cosmetic composition for whitening and improving the resilience of skin | |
| CN109350579B (en) | Whitening cosmetic additive, whitening skin-refreshing lotion and preparation method thereof | |
| CN109288770A (en) | A kind of anti-apolexis composition and its application | |
| CN103919704A (en) | Anti-aging essence | |
| CN102697701B (en) | Skin care composition with skin whitening and moisturizing efficacies as well as preparation method and applications thereof | |
| CN109453059A (en) | Skin-lightening cosmetic additive and whitening sleep mask and preparation method thereof | |
| CN110960455A (en) | Essence composition with whitening effect and preparation method and application thereof | |
| CN105943427B (en) | A kind of multiple-effect containing peony seed oil moistens eye cream and preparation method thereof | |
| CN108852969B (en) | Anti-wrinkle anti-aging whitening mask and preparation method thereof | |
| CN107440996A (en) | A kind of skin care item containing B B-complex and preparation method thereof | |
| CN109453056A (en) | A kind of moisture saver mask liquid and preparation method thereof | |
| CN107961174A (en) | Lipstick containing stem cell secretion factors and preparation method thereof | |
| CN109846798B (en) | Composition for tightening skin and cosmetic containing same | |
| TW202106323A (en) | Use ofrosmarinus officinaliscallus extract for manufacturing composition inhibiting skin aging and a culture medium for inducingrosmarinus officinaliscallus | |
| CN101357114A (en) | Whitening antioxidation cosmetic compositions | |
| CN109453088A (en) | Compact frost of whitening and preparation method thereof and tyrosinase inhibitor | |
| CN109998941A (en) | Anti-apolexis composition, anti-ageing face mask and preparation method thereof | |
| CN116549322A (en) | Plasmin composition with whitening and freckle removing effects and application thereof | |
| CN111743798B (en) | Multi-effect anti-aging skin care product and preparation method thereof | |
| CN111603404B (en) | Skin care essence containing cell extracting solution and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160420 Termination date: 20171220 |