CN103690450B - There is the compositions of whitening anti-aging effect, skin care item and preparation method and application - Google Patents
There is the compositions of whitening anti-aging effect, skin care item and preparation method and application Download PDFInfo
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Abstract
The invention discloses compositions, skin care item and the preparation method and application with whitening anti-aging effect, described compositions makes by following component: AA 2G 0.2%-10.0%, superoxide dismutase 0.1%-0.2%, Semen Vitis viniferae extract 0.1%-0.5%, fermented soybean extract 0.1%-0.5%, propylene glycol 10%-30%, Aloe powder 0.1%-0.2%, surplus is deionized water.Experimental result shows, the compositions with whitening anti-aging effect of the present invention has effect of whitening anti-aging, and method of the present invention is simple to operate, and production cost is low, and product stability is good; In vitro tests and human body skin test evaluation confirm to have good whitening and senile-resistant efficacy, and safety has no side effect; Meanwhile, the compositions with whitening anti-aging effect provided by the invention and the skin care item containing said composition also have good long-acting moistening effect.
Description
Technical field
The present invention relates to a kind of skin care compositions and its application in cosmetics, particularly relate to a kind of there is whitening anti-aging effect skin care compositions and preparation method, skin care item containing this skin care compositions and preparation method.
Background technology
The theme that the beautiful and beautiful people of being pursue forever, main embodiment is exactly whitening and the slow down aging of pursuing human body skin, because skin is human body most surface is also maximum organ, but because skin directly contacts with external environment condition, be vulnerable to the stimulation of the factor such as various material, ray in environment, be easy to come to harm, make skin easily aging and damage, directly affects the attractive in appearance of human body.
Skin damage hinders the increase of free radical in disappearance that major embodiment is moisture in Skin Cell, the accumulation of metabolic waste, skin, the loss etc. of intracellular nutritional material.And these problems can have the tonic cosmetics of function to safeguard by use part and improve, the whitening therefore on market and anti-aging cosmetics are very by the favor of consumers in general.On market for whitening and antidotal cosmetics and raw material more, but in whitening with defying age aspect characteristic is stable, comprehensive antidotal skin care item are not a lot.
Superoxide dismutase (SOD) is the natural enemy of oxygen-derived free radicals, is the number one killer of oxygen-derived free radicals in body, and level height in vivo means index directly perceived that is old and feeble and death.SOD can suppress the strong oxidation of oxygen-derived free radicals at skin surface, reach the object of skin protection.SOD makes an addition in cosmetics and can effectively prevent skin to be subject to the damage of ionizing radiation, thus plays sun-proof result; Also effectively skin aging be can prevent, speckle dispelling, crease-resistant effect played; But SOD is poor stability in cosmetics, by timely and effective absorption, so mostly the SOD skin care item in market are concept type product, the effect reached in anticipation can not be difficult to.The superoxide dismutase that Zhong Xing energy company produces be fermentable then through the process of molecular level, there is high temperature resistant and resistance to light characteristic, biologically active tat 10000-15000U/mg, and comparatively stable, easily absorbed.There is good whitening and anti-oxidation efficacy.
Semen Vitis viniferae extract is the antioxidation that current occurring in nature finds, the material that Scavenging ability is the strongest, and its antioxidant activity is V
e50 times, V
c20 times, it can effectively remove free radical unnecessary in human body, has superpower slow down aging and the effect of enhancing immunity.Fermented soybean extract is rich in plant soybean isoflavone, there is similar human body estrogen action, pass through antioxidation, restraint of tyrosinase is active, show extremely strong whitening effect, also there is the effect promoting collagen protein and hyaluronic acid synthesis, make that skin is fine and smooth, pure white, high resilience and gloss.
The present invention, according to the function of each material and feature, by multicomponent proportion optimizing and extraction, obtains whitening anti-aging compositions, and is prepared into skin care item.
Summary of the invention
The object of this invention is to provide a kind of compositions with whitening anti-aging effect.
Second object of the present invention is to provide a kind of preparation method with the compositions of whitening anti-aging effect.
3rd object of the present invention is to provide a kind of skin care item of compositions containing having whitening anti-aging effect.
4th object of the present invention is to provide a kind of preparation method of skin care item of compositions containing having whitening anti-aging effect.
5th object of the present invention is to provide a kind of purposes with the compositions of whitening anti-aging effect.
6th object of the present invention is to provide a kind of purposes of skin care item of compositions containing having whitening anti-aging effect.
A kind of compositions with whitening anti-aging effect, make by following component: AA 2G 0.2%-10.0%, superoxide dismutase 0.1%-0.2%, Semen Vitis viniferae extract 0.1%-0.5%, fermented soybean extract 0.1%-0.5%, propylene glycol 10%-30%, Aloe powder 0.1%-0.2%, surplus is deionized water.
Described superoxide dismutase is that fermentable prepares, and biological activity is 10000-15000U/mg.
There is a preparation method for the compositions of whitening anti-aging effect, comprise the steps:
1) taking following component is by weight raw material: AA 2G 0.2%-10.0%, superoxide dismutase 0.1%-0.2%, Semen Vitis viniferae extract 0.1%-0.5%, fermented soybean extract 0.1%-0.5%, propylene glycol 10%-30%, Aloe powder 0.1%-0.2%, surplus is deionized water;
2) by AA 2G, superoxide dismutase, Semen Vitis viniferae extract and fermented soybean extract, join in the deionized water of raw materials quality 50%-60% described in described step 1), heating in water bath, to 60-70 DEG C, heats 10-20min;
3) Aloe powder is spilled in propylene glycol, is heated to 60-70 DEG C, stir 5-10min;
4) mixture that step 3) obtains is joined step 2) in the liquid that obtains, add deionized water to 100%, stir; Be heated to 85-90 DEG C, insulation 25-30min; Psychrolusia is cooled to less than 45 DEG C, obtains a kind of compositions with whitening anti-aging effect.
Described superoxide dismutase is that fermentable prepares, and biological activity is 10000-15000U/mg.
A kind of skin care item of compositions containing having whitening anti-aging effect, it is make by following component: disodiumedetate 0.02%, glycerol 5.0%-10.0%, 1,3-butanediol 4.0%-8.0%, glycyrrhizic acid dipotassium 4.0%-8.0%, xanthan gum 0.1%-0.3%, water solublity Jojoba oil 0.4%-0.8%, water-solubleazone 0.1%-0.2%, D-panthenol 0.1%-0.2%, has the compositions 20.0%-40.0% of whitening anti-aging effect, carbomer 0.5%, triethanolamine 0.5%-1.0%, essence 0.01%, PHG0.5%, surplus is deionized water.
Containing the preparation method of skin care item of compositions with whitening anti-aging effect, comprise the steps:
1) taking following component is by weight raw material:
Disodiumedetate 0.02%, glycerol 5.0%-10.0%, 1,3-butanediol 4.0%-8.0%, glycyrrhizic acid dipotassium 4.0%-8.0%, xanthan gum 0.1%-0.3%, water solublity Jojoba oil 0.4%-0.8%, water-solubleazone 0.1%-0.2%, D-panthenol 0.1%-0.2%, there is the compositions 20.0%-40.0% of whitening anti-aging effect, carbomer 0.5%, triethanolamine 0.5%-1.0%, essence 0.01%, PHG0.5%, surplus is deionized water;
2) carbomer is spilled in the deionized water of described step 1) raw materials quality 30%, leaves standstill 2-4h, carbomer is fully absorbed water swelling; Obtain mixture a, be heated to 60-70 DEG C;
3) disodiumedetate, glycyrrhizic acid dipotassium, glycerol, 1,3 butylene glycol are joined successively in the deionized water of described step 1) raw materials quality 10%, stir 10min-20min; Be heated to 60-70 DEG C, add water solublity Jojoba oil, water-solubleazone, D-panthenol successively, 400rpm-500rpm stirs 10min-20min and under 500rpm-600rpm stirring condition, is spilled into xanthan gum again, obtains mixture b;
4) mixture a, b are mixed with the compositions with whitening anti-aging effect, supplement deionized water to 98.49-98.99%; Under water-bath 70-80 DEG C condition, 300rpm-400rpm stirs 10min-20min, and dropwise adding triethanolamine, is gel to solution; 20min-30min is incubated under 80-85 DEG C of condition;
5) cold water is cooled to less than 40 DEG C, adds essence and antiseptic PHG, namely obtains a kind of skin care item of compositions containing having whitening anti-aging effect.
The compositions with whitening anti-aging effect has the purposes of whitening anti-aging skin care item in preparation.
Containing have whitening anti-aging effect compositions skin care item purposes.
Experimental result shows, of the present invention have effect that the compositions of whitening anti-aging effect and the skin care item containing the compositions with whitening anti-aging effect have whitening anti-aging, and for making substrate effect without whitening anti-aging of skin care item, illustrate that the present composition is suitable as cosmetic industry composition and adds use.
A kind of preparation method with the compositions of whitening anti-aging effect of the present invention is simple to operate, and production cost is low, and product stability is good; The compositions with whitening anti-aging effect provided by the invention is the bright liquid of colourless or lurid clarification; The compositions with whitening anti-aging effect provided by the invention and the skin care item containing said composition confirm to have good whitening and senile-resistant efficacy through in vitro tests and human body skin test evaluation, and safety has no side effect; Meanwhile, the compositions with whitening anti-aging effect provided by the invention and the skin care item containing said composition also have good long-acting moistening effect.
Accompanying drawing explanation
Fig. 1 is that the compositions with whitening anti-aging effect of the embodiment of the present invention 4,5,6 preparation is to the maximum inhibition curve of TYR enzyme;
Fig. 2 is that the compositions with whitening anti-aging effect prepared of the method for the embodiment of the present invention 4 is to the clearance rate numerical value figure of DPPH free radical;
Fig. 3 is a kind of human body skin roughness Assessment of Changes figure of skin care item of compositions containing having whitening anti-aging effect prepared by the method for the embodiment of the present invention 10;
Fig. 4 is a kind of human body skin Flexible change evaluation map of skin care item of compositions containing having whitening anti-aging effect prepared by the method for the embodiment of the present invention 10.
Detailed description of the invention
The test method used in following embodiment if no special instructions, is conventional method.
Material used in following embodiment, reagent etc., if no special instructions, all can obtain from commercial channels.The source of following raw material is only in order to the present invention is described, does not limit the invention, and may be used for the present invention with the same or analogous product of the product specification in following source.
Superoxide dismutase is that fermentable prepares (in the emerging energy (Tianjin) company limited produce), and biological activity is 10000-15000U/mg.
Semen Vitis viniferae extract (being purchased from Yuan Sen bio tech ltd, Xi'an).
Fermented soybean extract (in the emerging energy (Tianjin) company limited produce).
Aloe powder (the international holding company of U.S.'s thyrite produces)
PHG is compound preservative (be 65% phenoxyethanol by mass ratio, 35% ethohexadiol is composite to be formed).PHG is
pHG(composition: phenoxyethanol PHENOXYETHANOL, 1,2 ethohexadiol CAPRYLYLGLYCOL), for THOR company produces phenoxyethanol class antiseptic, purchased from the Shanghai universe one Chemical Company.
Embodiment 1
A kind of compositions with whitening anti-aging effect, make by following component: AA 2G 5g, superoxide dismutase 0.1g, Semen Vitis viniferae extract 0.3g, fermented soybean extract 0.3g, propylene glycol 20g, Aloe powder 0.1g, deionized water 74.2g, superoxide dismutase is wherein that fermentable prepares, and biological activity is 13000U/mg.
Embodiment 2
There is the compositions of whitening anti-aging effect, make by following component: AA 2G 0.2g, superoxide dismutase 0.2g, Semen Vitis viniferae extract 0.1g, fermented soybean extract 0.5g, propylene glycol 10g, Aloe powder 0.2g, deionized water 88.8g.Superoxide dismutase is wherein that fermentable prepares, and biological activity is 10000U/mg.
Embodiment 3
There is the compositions of whitening anti-aging effect, make by following component: AA 2G 10.0g, superoxide dismutase 0.1g, Semen Vitis viniferae extract 0.5g, fermented soybean extract 0.1g, propylene glycol 30g, Aloe powder 0.1g, deionized water 59.2g.Superoxide dismutase is wherein that fermentable prepares, and biological activity is 15000U/mg.
Embodiment 4
There is a preparation method for the compositions of whitening anti-aging effect, comprise the steps:
1) taking following component is by weight raw material: AA 2G 5g, superoxide dismutase 0.1g, Semen Vitis viniferae extract 0.3g, fermented soybean extract 0.3g, propylene glycol 20g, Aloe powder 0.1g, deionized water 74.2g, superoxide dismutase is wherein that fermentable prepares, and biological activity is 13000U/mg;
2) by AA 2G, superoxide dismutase, Semen Vitis viniferae extract and fermented soybean extract, add in 55g deionized water, heating in water bath to 65 DEG C, heating 15min;
3) Aloe powder is spilled in propylene glycol, is heated to 65 DEG C, stir 8min;
4) mixture that step 3) obtains is joined step 2) in the liquid that obtains, add deionized water to 100g, stir; Be heated to 88 DEG C, insulation 28min; Psychrolusia is cooled to less than 45 DEG C, obtains a kind of compositions with whitening anti-aging effect.
In order to prevent above-mentioned composition corrupt, 0.5g antiseptic phenoxyethanol (Phenoxyethanol) can also be joined in the compositions of above-mentioned 100g.
Above-mentioned composition will be preserved under room temperature lucifuge condition, for subsequent use.
Embodiment 5
There is a preparation method for the compositions of whitening anti-aging effect, comprise the steps:
1) taking following component is by weight raw material: AA 2G 0.2g, superoxide dismutase 0.2g, Semen Vitis viniferae extract 0.1g, fermented soybean extract 0.5g, propylene glycol 10g, Aloe powder 0.2g, deionized water 88.8g.Superoxide dismutase is wherein that fermentable prepares, and biological activity is 10000U/mg;
2) by AA 2G, superoxide dismutase, Semen Vitis viniferae extract and fermented soybean extract, add in 60g deionized water, heating in water bath to 70 DEG C, heating 10min;
3) Aloe powder is spilled in propylene glycol, is heated to 70 DEG C, stir 5min;
4) mixture that step 3) obtains is joined step 2) in the liquid that obtains, add deionized water to 100g, stir; Be heated to 90 DEG C, insulation 25min; Psychrolusia is cooled to less than 45 DEG C, obtains a kind of compositions with whitening anti-aging effect.
In order to prevent above-mentioned composition corrupt, 0.5g antiseptic phenoxyethanol (Phenoxyethanol) can also be joined in the compositions of above-mentioned 100g.
Above-mentioned composition will be preserved under room temperature lucifuge condition, for subsequent use.
Embodiment 6
There is a preparation method for the compositions of whitening anti-aging effect, comprise the steps:
1) taking following component is by weight raw material: AA 2G 10.0g, superoxide dismutase 0.1g, Semen Vitis viniferae extract 0.5g, fermented soybean extract 0.1g, propylene glycol 30g, Aloe powder 0.1g, deionized water 59.2g.Superoxide dismutase is wherein that fermentable prepares, and biological activity is 15000U/mg;
2) by AA 2G, superoxide dismutase, Semen Vitis viniferae extract and fermented soybean extract, add in 50g deionized water, heating in water bath to 60 DEG C, heating 20min;
3) Aloe powder is spilled in propylene glycol, is heated to 60 DEG C, stir 10min;
4) mixture that step 3) obtains is joined step 2) in the liquid that obtains, add deionized water to 100g, stir; Be heated to 85 DEG C, insulation 30min; Psychrolusia is cooled to less than 45 DEG C, obtains a kind of compositions with whitening anti-aging effect.
In order to prevent above-mentioned composition corrupt, 0.5g antiseptic phenoxyethanol (Phenoxyethanol) can also be joined in the compositions of above-mentioned 100g.
Above-mentioned composition will be preserved under room temperature lucifuge condition, for subsequent use.
Embodiment 7
Containing skin care item with the compositions of whitening anti-aging effect prepared by embodiment 4, make by following component:
Disodiumedetate 0.02g, glycerol 8.0g, 1,3-butanediol 6.0g, glycyrrhizic acid dipotassium 6.0g, xanthan gum 0.2g, water solublity Jojoba oil 0.6g, water-solubleazone 0.2g, D-panthenol 0.2g, the compositions 30.0g with whitening anti-aging effect prepared by embodiment 4, carbomer 0.5g, triethanolamine 0.8g, essence 0.01g, PHG0.5g, deionized water 46.97g.
Embodiment 8
Containing skin care item with the compositions of whitening anti-aging effect prepared by embodiment 5, make by following component:
Disodiumedetate 0.02g, glycerol 5.0g, 1,3-butanediol 8.0g, glycyrrhizic acid dipotassium 4.0g, xanthan gum 0.3g, water solublity Jojoba oil 0.4g, water-solubleazone 0.2g, D-panthenol 0.1g, the compositions 40.0g with whitening anti-aging effect prepared by embodiment 5, carbomer 0.5g, triethanolamine 0.5g, essence 0.01g, PHG0.5g, deionized water 40.47g.
Embodiment 9
Containing skin care item with the compositions of whitening anti-aging effect prepared by embodiment 6, make by following component:
Disodiumedetate 0.02g, glycerol 10.0g, 1,3-butanediol 4.0g, glycyrrhizic acid dipotassium 8.0g, xanthan gum 0.1g, water solublity Jojoba oil 0.8g, water-solubleazone 0.1g, D-panthenol 0.2g, the compositions 20.0g with whitening anti-aging effect prepared by embodiment 6, carbomer 0.5g, triethanolamine 1.0g, essence 0.01g, PHG0.5g, deionized water 45.23g.
Embodiment 10
Containing the preparation method of skin care item of compositions with whitening anti-aging effect, comprise the steps:
1) taking following component is by weight raw material:
Disodiumedetate 0.02g, glycerol 8.0g, 1,3-butanediol 6.0g, glycyrrhizic acid dipotassium 6.0g, xanthan gum 0.2g, water solublity Jojoba oil 0.6g, water-solubleazone 0.2g, D-panthenol 0.2g, the compositions 30.0g with whitening anti-aging effect prepared by embodiment 4, carbomer 0.5g, triethanolamine 0.8g, essence 0.01g, PHG0.5g, deionized water 46.97g;
2) carbomer is spilled in the deionized water of 30g, leaves standstill 3h, carbomer is fully absorbed water swelling; Obtain mixture a, be heated to 65 DEG C;
3) disodiumedetate, glycyrrhizic acid dipotassium, glycerol, 1,3 butylene glycol are joined in 10g deionized water successively, stir 15min; Be heated to 65 DEG C, add water solublity Jojoba oil, water-solubleazone, D-panthenol successively, 500rpm stirs 15min and under 600rpm stirring condition, is spilled into xanthan gum again, obtains mixture b;
4) mixture a, b are mixed with the compositions with whitening anti-aging effect, supplement deionized water to 98.69g; Under water-bath 75 DEG C of conditions, 400rpm stirs 15min, and dropwise adding triethanolamine, is gel to solution; 25min is incubated under 83 DEG C of conditions;
5) cold water is cooled to less than 40 DEG C, adds essence and antiseptic PHG, namely obtains a kind of skin care item of compositions containing having whitening anti-aging effect.
Embodiment 11
Containing the preparation method of skin care item of compositions with whitening anti-aging effect, comprise the steps:
1) taking following component is by weight raw material:
Disodiumedetate 0.02g, glycerol 5.0g, 1,3-butanediol 8.0g, glycyrrhizic acid dipotassium 4.0g, xanthan gum 0.3g, water solublity Jojoba oil 0.4g, water-solubleazone 0.2g, D-panthenol 0.1g, the compositions 40.0g with whitening anti-aging effect prepared by embodiment 5, carbomer 0.5g, triethanolamine 0.5g, essence 0.01g, PHG0.5g, deionized water 40.47g
2) carbomer is spilled in the deionized water of 30g, leaves standstill 2h, carbomer is fully absorbed water swelling; Obtain mixture a, be heated to 70 DEG C;
3) disodiumedetate, glycyrrhizic acid dipotassium, glycerol, 1,3 butylene glycol are joined in 10g deionized water successively, stir 10min; Be heated to 70 DEG C, add water solublity Jojoba oil, water-solubleazone, D-panthenol successively, 400rpm stirs 20min and under 500rpm stirring condition, is spilled into xanthan gum again, obtains mixture b;
4) mixture a, b are mixed with the compositions with whitening anti-aging effect, supplement deionized water to 98.99g; Under water-bath 80 DEG C of conditions, 300rpm stirs 10min, and dropwise adding triethanolamine, is gel to solution; 20min is incubated under 85 DEG C of conditions;
5) cold water is cooled to less than 40 DEG C, adds essence and antiseptic PHG, namely obtains a kind of skin care item of compositions containing having whitening anti-aging effect.
Embodiment 12
Containing the preparation method of skin care item of compositions with whitening anti-aging effect, comprise the steps:
1) taking following component is by weight raw material:
Disodiumedetate 0.02g, glycerol 10.0g, 1,3-butanediol 4.0g, glycyrrhizic acid dipotassium 8.0g, xanthan gum 0.1g, water solublity Jojoba oil 0.8g, water-solubleazone 0.1g, D-panthenol 0.2g, the compositions 20.0g with whitening anti-aging effect prepared by embodiment 6, carbomer 0.5g, triethanolamine 1.0g, essence 0.01g, PHG0.5g, deionized water 45.23g;
2) carbomer is spilled in the deionized water of 30g, leaves standstill 4h, carbomer is fully absorbed water swelling; Obtain mixture a, be heated to 60 DEG C;
3) disodiumedetate, glycyrrhizic acid dipotassium, glycerol, 1,3 butylene glycol are joined in 10g deionized water successively, stir 20min; Be heated to 60 DEG C, add water solublity Jojoba oil, water-solubleazone, D-panthenol successively, 500rpm stirs 10min and under 600rpm stirring condition, is spilled into xanthan gum again, obtains mixture b;
4) mixture a, b are mixed with the compositions with whitening anti-aging effect, supplement deionized water to 98.49g; Under water-bath 70 DEG C of conditions, 400rpm stirs 20min, and dropwise adding triethanolamine, is gel to solution; 30min is incubated under 80 DEG C of conditions;
5) cold water is cooled to less than 40 DEG C, adds essence and antiseptic PHG, namely obtains a kind of skin care item of compositions containing having whitening anti-aging effect.
Embodiment 13
The white-skinned face function test with the compositions of whitening anti-aging effect of embodiment 4,5,6 preparation: TYR inhibition of enzyme activity is tested.
Tryrosinase is rate-limiting enzyme main in melanin forming process, and the active size of this enzyme decides the quantity of melanin formation, and tyrosinase activity is high, namely generates with its product melanin of tyrosine effect and increases, find expression on skin of face and produce various black speck.Therefore, restraint of tyrosinase activity effectively reduces a kind of approach of melanin generation.Can catalytic reaction be there is in TYR enzyme and its substrate TYR, when after the reagent being added with the effect of TYR inhibition of enzyme activity in reaction system, inhibitory action can be produced to catalytic reaction, by measure be added with the effect of TYR inhibition of enzyme activity reagent before and after the change of 475nm place absorbance, evaluating this has the reagent of TYR inhibition of enzyme activity effect to the suppression ratio of TYR enzymatic activity.
Concrete process of the test is as follows:
1) take 17.91g disodium hydrogen phosphate to be dissolved in 500mL distilled water, obtain solution a; Take 7.8g sodium dihydrogen phosphate dihydrate to be dissolved in 500mL distilled water, obtain solution b; 92.6mL solution a and 107.4mL solution b is mixed, obtains the PBS buffer solution that 200mLpH is 6.8;
2) take 0.05gL-tyrosine, first use the dissolving with hydrochloric acid of a small amount of 0.1mol/L, after it dissolves, be settled to 100mL with the PBS buffer that the pH of above-mentioned preparation is 6.8;
In process of the test, in each group, the addition of each material is as shown in table 1.
Table 1 has the compositions of whitening anti-aging effect to the addition of material each in the active suppression test of TYR enzyme
Note: C
1group and T
1group all adds 0.5mL tryrosinase, and enzyme is lived as 100U/mL.
3) C
0organize after each solution prepares and shake up, heating in water bath 10min in 37 DEG C of water-baths, returns to zero under wavelength 475nm.C
1after group solution prepares and shakes up, add 0.5ml enzyme after 37 DEG C of water-bath 10min and live as the tryrosinase of 100U/mL, continue water-bath 10 minutes, measure absorbance;
4) T
0organize after each solution prepares and shake up, heating in water bath 10min in 37 DEG C of water-baths, returns to zero under wavelength 475nm.T
1after group solution prepares and shakes up, add 0.5ml enzyme after 37 DEG C of water-bath 10min and live as the tryrosinase of 100U/mL, continue water-bath 10 minutes, measure absorbance;
5) the maximum inhibition T(% of tryrosinase is calculated), T(%)=(C
1-T
1)/C
1× 100%.
Result of the test as shown in Figure 1.Test group 1,2,3 is corresponding embodiment 4,5,6 respectively, and when the compositions with whitening anti-aging effect is obvious to tyrosinase activity inhibition, and suppression ratio increases with the increase of composition concentration; Wherein, when composition concentration is 10%, inhibitory activity against tyrosinase meansigma methods is 59.1%, and peak reaches 64.8%; When concentration is 30% concentration, suppression ratio reaches 90.5%, and when concentration is 40% concentration, suppression ratio reaches 92.1%.Result display whitening anti-aging compositions is obvious to tyrosinase activity inhibition, and it has significant whitening effect.
Embodiment 14
Embodiment 12 prepare a kind of containing have whitening anti-aging effect compositions skin care item white-skinned face function test
Human body white-skinned face function is tested, and adopts human test's mode, measures melanin content and the brightness of skin with the dark red pigment tester of skin and skin colourity test probe.
Choose 30 volunteers, in 20-40 year, men and women half and half, within before recipient site 2 ~ 3 days, can not use any product; With dry medicated napkin, both hands inner forearm wiped clean is 4 × 4cm
2pilot region measures labelling, measures skin brightness and the melanin content of tested skin with skin colourity test probe+MPA9 tester and skin dark red pigment tester; Sample and blank group (blank group is the substrate not containing whitening anti-aging compositions) will be tested by 2.0 ± 0.1mg sample/cm with latex finger cot
2consumption spread upon respectively in left and right arm, smear and measure the skin brightness in 1st ~ 4 weeks each regions and melanin content respectively afterwards.
(1) mutation analysis of skin brightness
Skin brightness change is reflected in test period, investigates Experimental Area skin brightness L-value Changing Pattern in time.Its value is larger, and skin brightness is larger, otherwise skin brightness is lower.L-value (T before each time point L-value (Tn)-use product after skin brightness difference (Δ T)=use product
0).
The difference of table 2 recipient site skin brightness L-value and initial value
In test during 4 weeks, after the blank group of volunteer's recipient site dermal application, skin brightness numerical value does not significantly change, and illustrates that the substrate of smearing does not have the ability improving skin brightness.And at test period, the difference of sample sets recipient site skin brightness L-value and initial value be always on the occasion of, and skin brightness difference raises gradually along with the prolongation of testing time, shows that the skin care item containing whitening anti-aging compositions have obvious whitening effect.
(2) melanin content mutation analysis
Dermal melanin changes of contents is reflected in test period, pilot region dermal melanin content Changing Pattern in time.Its value is larger, and dermal melanin content is larger, otherwise, dermal melanin content.Melanin content (T0) before each time point melanin content (Tn)-use product after melanin content difference (Δ T)=use product.
The difference of table 3 recipient site dermal melanin content and initial value
In test during 4 weeks, after the blank group of volunteer's recipient site dermal application, the melanin content contained in skin on the occasion of, and numerical value is without significant change, illustrates that the blank group substrate of smearing is not eliminated or shifts melanic ability.And at test period, the melanin content of sample sets recipient site skin and the difference of initial value are negative value always, and the skin care item of a kind of compositions containing having whitening anti-aging effect prepared by interpret sample group and embodiment 12 have the ability reducing or shift melanin content; And melanin reduces gradually containing the prolongation changed in time, shows that sample sets has obvious whitening effect.
Experiment proves, embodiment 10 has the effect similar to skin care item prepared by embodiment 12 with the skin care item containing the compositions with whitening anti-aging effect prepared by embodiment 11.
Embodiment 15
The senile-resistant efficacy with the compositions of whitening anti-aging effect prepared by the method for embodiment 4 is tested: remove the experiment of DPPH free radical.
DPPH is a kind of very stable free radical, if compositions can be removed, then represents that compositions has the effect of the valid density reducing hydroxy radical or oxyradical.DPPH has a characteristic absorption peak at 517nm place, and when running into free radical scavenger, DPPH diminishes at the absorbance of maximum absorption wave strong point.Therefore, its elimination effect to DPPH is evaluated by measuring the change of absorbance.
Accurately take 20mgDPPH, be settled in 250ml volumetric flask with dehydrated alcohol, obtain the DPPH solution that concentration is 20mmol/L, compositions is diluted to the test fluid of variable concentrations.Get test fluid and the 2ml20mmol/LDPPH of 2ml variable concentrations respectively, react 30min after mixing, measure the absorbance under 517nm wavelength, control solvent dehydrated alcohol replaces, and is calculated as follows suppression ratio.Suppression ratio (%)=[1-(Ai-Aj/Ac] × 100; Wherein, Ai is the absorbance of the mixed liquor that the test fluid of 2mlDPPH solution and 2ml variable concentrations forms; Aj is the absorbance of the mixed liquor that the test fluid of 2ml variable concentrations and 2ml dehydrated alcohol form; Ac is the absorbance of the mixed liquor that 2mlDPPH solution and 2ml dehydrated alcohol form.
Compositions is comparatively obvious to DPPH scavenging action, and as shown in Figure 2, the composition solution Scavenging activity of low concentration is more weak, but along with the increase of concentration, DPPH free radical scavenging activity increases thereupon.When composition concentration is 20%, clearance rate reaches 64%; When concentration is 40%, clearance rate reaches 80%; Result shows, when composition concentration is at 20%-40%, whitening anti-aging composition removing DPPH free radical Be very effective, has obvious antidotal effect.
Embodiment 16
A kind of skin care item skin elasticity testing experiment of compositions containing having whitening anti-aging effect prepared by the method for embodiment 10, detailed process is as follows:
Use German Khazaka(CK)+Courage company skin elasticity tester MPA580 and ReviscometerRV600 pop one's head in 30 volunteers, 30-50 year, men and women half and half, and recipient site face is tested, detection of skin elasticity and roughness parameter.
Smearing in 4 weeks, detect once weekly, and grade to skin elasticity and roughness, its empty group is not containing the substrate of whitening anti-aging compositions.
As Fig. 3 and Fig. 4, result shows, in 4 weeks that detect, the skin average roughness parameter of blank sample is used not have significant change, the skin of test group personnel is along with the prolongation of service time, and skin coarseness is on a declining curve, and when the 4th week, skin mean roughness declines and reaches 36%; Carry out observation to subject's face to detect, skin surface microgroove attenuates little, and smoothness strengthens, and shows that skin care item of the present invention significantly can improve the roughness of human body skin.In 4 weeks that detect, use the skin average roughness parameter of blank sample to remain basically stable, skin elasticity is increase trend with the prolongation of service time, when the 4th week, coefficient of skin elasticity value is 7.9, adds 21%, show that the skin elasticity of subject is significantly improved than the 0th week numerical value.
Experiment shows, the skin care item of a kind of compositions containing having whitening anti-aging effect prepared by the method for embodiment 11,12 have similar effect to skin care item prepared by the method for embodiment 10.
The compositions of whitening anti-aging effect method routinely can also prepare emulsion, essence, essence or cream frost etc.
Claims (7)
1. one kind has the compositions of whitening anti-aging effect, it is characterized in that making by following component: L-AA-2-glucoside 0.2%-10.0%, superoxide dismutase 0.1%-0.2%, Semen Vitis viniferae extract 0.1%-0.5%, fermented soybean extract 0.1%-0.5%, propylene glycol 10%-30%, Aloe powder 0.1%-0.2%, surplus is deionized water.
2. a kind of compositions with whitening anti-aging effect according to claim 1, it is characterized in that, described superoxide dismutase is that fermentable prepares, and biological activity is 10000-15000U/mg.
3. a kind of preparation method with the compositions of whitening anti-aging effect of claim 1, is characterized in that comprising the steps:
1) taking following component is by weight raw material: L-AA-2-glucoside 0.2%-10.0%, superoxide dismutase 0.1%-0.2%, Semen Vitis viniferae extract 0.1%-0.5%, fermented soybean extract 0.1%-0.5%, propylene glycol 10%-30%, Aloe powder 0.1%-0.2%, surplus is deionized water;
2) by L-AA-2-glucoside, superoxide dismutase, Semen Vitis viniferae extract and fermented soybean extract, join step 1) in the deionized water of composition quality 50%-60%, heating in water bath to 60-70 DEG C, heating 10-20min;
3) Aloe powder is spilled in propylene glycol, is heated to 60-70 DEG C, stir 5-10min;
4) by step 3) mixture that obtains joins step 2) in the liquid that obtains, add deionized water and make compositions be 100%, stir; Be heated to 85-90 DEG C, insulation 25-30min; Psychrolusia is cooled to less than 45 DEG C, obtains a kind of compositions with whitening anti-aging effect.
4. a kind of preparation method with the compositions of whitening anti-aging effect according to claim 3, it is characterized in that superoxide dismutase is that fermentable prepares, biological activity is 10000-15000U/mg.
5. the skin care item with the compositions of whitening anti-aging effect containing claim 1 or 2, it is characterized in that making by following component: disodiumedetate 0.02%, glycerol 5.0%-10.0%, 1, 3-butanediol 4.0%-8.0%, glycyrrhizic acid dipotassium 4.0%-8.0%, xanthan gum 0.1%-0.3%, water solublity Jojoba oil 0.4%-0.8%, water-solubleazone 0.1%-0.2%, D-panthenol 0.1%-0.2%, there is the compositions 20.0%-40.0% of whitening anti-aging effect, carbomer 0.5%, triethanolamine 0.5%-1.0%, essence 0.01%, PHG0.5%, surplus is deionized water, PHG is compound preservative, is 65% phenoxyethanol by mass ratio, and 35% ethohexadiol is composite to be formed.
6. claim 5 one kind is containing the preparation method of skin care item of compositions with whitening anti-aging effect, it is characterized in that comprising the steps:
1) taking following component is by weight raw material:
Disodiumedetate 0.02%, glycerol 5.0%-10.0%, 1,3-butanediol 4.0%-8.0%, glycyrrhizic acid dipotassium 4.0%-8.0%, xanthan gum 0.1%-0.3%, water solublity Jojoba oil 0.4%-0.8%, water-solubleazone 0.1%-0.2%, D-panthenol 0.1%-0.2%, there is the compositions 20.0%-40.0% of whitening anti-aging effect, carbomer 0.5%, triethanolamine 0.5%-1.0%, essence 0.01%, PHG0.5%, surplus is deionized water;
2) carbomer is spilled in the deionized water of raw material of skin care articles quality 30%, leaves standstill 2-4h, make the abundant water absorption and swelling of carbomer; Obtain mixture a, be heated to 60-70 DEG C;
3) disodiumedetate, glycyrrhizic acid dipotassium, glycerol, 1,3 butylene glycol are joined successively in the deionized water of described raw material of skin care articles quality 10%, stir 10min-20min; Be heated to 60-70 DEG C, add water solublity Jojoba oil, water-solubleazone, D-panthenol successively, 400rpm-500rpm stirs 10min-20min and under 500rpm-600rpm stirring condition, is spilled into xanthan gum again, obtains mixture b;
4) mixture a, b are mixed with the compositions with whitening anti-aging effect, supplement deionized water skin care item to 98.49-98.99%; Under water-bath 70-80 DEG C condition, 300rpm-400rpm stirs 10min-20min, and dropwise adding triethanolamine, is gel to solution; 20min-30min is incubated under 80-85 DEG C of condition;
5) cold water is cooled to less than 40 DEG C, adds essence and antiseptic PHG, namely obtains a kind of skin care item of compositions containing having whitening anti-aging effect.
7. compositions according to claim 1 and 2 has the purposes of whitening anti-aging skin care item in preparation.
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| CN104738635B (en) * | 2015-04-13 | 2017-08-29 | 广州赛莱拉干细胞科技股份有限公司 | A kind of oxidation resistant composition and preparation method thereof |
| CN106265372B (en) * | 2016-09-30 | 2021-05-11 | 广东丸美生物技术股份有限公司 | Skin care matrix with anti-aging effect and preparation method and application thereof |
| CN108379170A (en) * | 2018-05-08 | 2018-08-10 | 广州奥蓓斯化妆品有限公司 | A kind of composite skin care product of skin whitening |
| CN108514519A (en) * | 2018-05-11 | 2018-09-11 | 珠海伊斯佳科技股份有限公司 | A kind of skin care compositions and methods and its application method of whitening and anti-aging |
| CN110960441B (en) * | 2019-12-12 | 2022-09-13 | 宁波久瑞生物工程有限公司 | SOD composition with stable activity and preparation process and application thereof |
| CN112891270B (en) * | 2021-01-29 | 2023-02-07 | 洪怡 | Cosmetic composition and preparation method and application thereof |
| CN113952250B (en) * | 2021-11-02 | 2023-11-21 | 上海忱扬生物科技有限公司 | Stable photo-aging resistant composition and preparation method thereof |
| CN115040446A (en) * | 2022-06-24 | 2022-09-13 | 曾会明 | SOD plant extract skin care composition, skin care product and preparation method thereof |
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| WO1999033439A1 (en) * | 1997-12-24 | 1999-07-08 | Shaklee Corporation | Composition with high efficiency skin protection from damaging effects of ultraviolet light |
| CN101184469A (en) * | 2005-04-28 | 2008-05-21 | 独立行政法人科学技术振兴机构 | Skin regeneration promoter |
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