CN105496864A - Nutritional skin care lotion containing oat peptide and preparation method and application of nutritional skin care lotion - Google Patents
Nutritional skin care lotion containing oat peptide and preparation method and application of nutritional skin care lotion Download PDFInfo
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
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- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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Abstract
The invention discloses a nutritional skin care lotion containing oat peptide and a preparation method and an application of the nutritional skin care lotion. The nutritional skin care lotion comprises components as follows: oat peptide, methyl hydroxybenzoate, water, magnesium aluminum silicate, butylene glycol, glycerin, propylene glycol, sodium hyaluronate, xanthan gum, squalane, 2-ethylhexyl isohexadecanoate, polydimethylsiloxane, butylated hydroxytoluene, propyl hydroxybenzoate, cetearyl alcohol, glyceryl stearate, PEG-100 (polyethylene glycol-100) stearate, sucrose stearate, cetearyl glucoside, cetyl alcohol, vitamin E, camellia seed oil, hydrolyzed cod fish skin protein, sugar beet root extract, carboxymethyl chitosan, a preservative and essence. The components are uniformly mixed and blended, and the nutritional skin care lotion containing oat peptide is obtained. The nutritional lotion has the functions of nourishing the skin, resisting wrinkles and resisting ageing, has mild and stable properties, is high in safety, has no side effects on the skin and is suitable for being used for a long time.
Description
Technical field
The invention belongs to daily chemical products field, particularly a kind of nutrition skin-protection emulsion containing oat peptide and preparation method thereof and application.
Background technology
The air pollution aggravated gradually along with society and the life stress day by day increased, the series of symptoms such as increasing application on human skin appearance is obscure, responsive, mistake presenility.Along with people increase the attention degree of skin nursing problem, people also improve constantly the function of skin-protection product and the requirement of quality, have efficient moisture-retention so a, wrinkle resistant defying age, and the facial treatment milk of whitening and skin-protecting feature has the larger market demand.
Traditional whitening and skin-protecting product, while reaching whitening effect, not obvious to the Nutrition of skin, sometimes even there is certain toxic and side effects to human body, less to the nursing role of skin, be not suitable for long-term excessively use.
At present, in order to solve the weak point that traditional whitening and skin-protecting product exists, people also prepare nutrition skin-protection product by nutritional labeling.But in the preparation process of nutrition skin-protection product, in order to the stability of nutrition skin-protection product protecting the activity of nutritional labeling and finally obtain, the selection of the constituent of nutrition skin-protection product is very crucial factor.
Summary of the invention
Primary and foremost purpose of the present invention is that the shortcoming overcoming prior art is with not enough, provides a kind of nutrition skin-protection emulsion containing oat peptide.
Another object of the present invention is to the preparation method that the described nutrition skin-protection emulsion containing oat peptide is provided.
Another object of the present invention is the application providing the described nutrition skin-protection emulsion containing oat peptide.
Object of the present invention is achieved through the following technical solutions: a kind of nutrition skin-protection emulsion containing oat peptide, is grouped into by following one-tenth by mass percentage:
Component A: oat peptide 50 ~ 77%, methyl hydroxybenzoate 0.05 ~ 0.1%;
B component: water 8 ~ 8.5%, Magnesiumaluminumsilicate 0.5 ~ 2%;
Component C: butanediol 2 ~ 3%, glycerol 1 ~ 2%, propylene glycol 2 ~ 3%, hyaluronate sodium 0.001 ~ 0.01%, xanthan gum 0.1 ~ 0.3%;
D component: squalane 1 ~ 5%, different ethylhexyl palmitate 1 ~ 2%, polydimethylsiloxane 1 ~ 5%, butylated hydroxytoluene 0.01 ~ 0.05%, propyl hydroxybenzoate 0.01 ~ 0.05%, cetearyl alcohol 1 ~ 5%, glyceryl stearate 0.5 ~ 1%, PEG-100 stearate (polyoxyethylene 100 stearate) 0.5 ~ 1%, sucrose stearate 0.3 ~ 0.6%, cetearyl glucoside 0.3 ~ 0.6%, spermol 0.4 ~ 0.8%, vitamin E 0.1 ~ 0.3%, Camellia oil 0.1 ~ 1%,
Component E: hydrolysis gadid hide collagen 1 ~ 2%, beet root extract 1 ~ 2%, carboxymethylchitosan 1 ~ 2%, antiseptic 0.1 ~ 0.6%, essence 0.1 ~ 1%.
The mass percentage of described oat peptide is preferably surplus, thus the described nutrition skin-protection emulsion containing oat peptide is 100%.
The mass percentage of described methyl hydroxybenzoate is preferably 0.1%.
Described oat peptide, hydrolysis gadid hide collagen, beet root extract and carboxymethylchitosan buying are from Guangzhou Run Pusen Chemical Industry Science Co., Ltd.
Described antiseptic is one in 2-bromo-2-nitro-1,3-propylene glycol, methylchloroisothiazandnone and Methylisothiazolinone or at least two kinds; Be preferably 2-bromo-2-nitro-1,3-propylene glycol.
The preparation method of the described nutrition skin-protection emulsion containing oat peptide, comprises the steps:
(1) prewired B phase: be added to the water by Magnesiumaluminumsilicate, is stirred to Magnesiumaluminumsilicate complete swelling even, obtains B phase, for subsequent use;
(2) prewired C phase: the mixing of butanediol, glycerol, propylene glycol, hyaluronate sodium and xanthan gum is infiltrated evenly, obtains C phase, for subsequent use;
(3) prewired D phase: squalane, different ethylhexyl palmitate, polydimethylsiloxane, butylated hydroxytoluene, propyl hydroxybenzoate, cetearyl alcohol, glyceryl stearate, PEG-100 stearate, sucrose stearate, cetearyl glucoside, spermol, vitamin E and Camellia oil are added oil phase reactor successively, stir while be heated to 78 ~ 80 DEG C, then in 78 ~ 80 DEG C of insulated and stirred to being dissolved into liquid completely, obtain D phase, be incubated for subsequent use;
(4) preformulation A+B+C phase: add oat peptide and methyl hydroxybenzoate in emulsifying pot, agitating heating, adds pre-assigned B phase, homogenizing; Add pre-assigned C phase, homogenizing; Be heated to 82 ~ 85 DEG C, homogenizing is to being uniformly dissolved completely, and insulation, obtains A+B+C phase, for subsequent use;
(5) preparation of nutrition skin-protection emulsion:
1. under vacuum, be added to by D in A+B+C phase, after adding, homogenizing is even to complete emulsifying, is incubated 10 ~ 15 minutes, lowers the temperature after froth breaking;
2. be cooled to less than 45 DEG C, add hydrolysis gadid hide collagen, beet root extract, carboxymethylchitosan, antiseptic and essence successively, after stirring under vacuum, homogenizing, obtains the nutrition skin-protection emulsion containing oat peptide;
Wherein, the mass percentage of each raw material is as follows:
Component A: oat peptide 50 ~ 77%, methyl hydroxybenzoate 0.05 ~ 0.1%;
B component: water 8 ~ 8.5%, Magnesiumaluminumsilicate 0.5 ~ 2%;
Component C: butanediol 2 ~ 3%, glycerol 1 ~ 2%, propylene glycol 2 ~ 3%, hyaluronate sodium 0.001 ~ 0.01%, xanthan gum 0.1 ~ 0.3%;
D component: squalane 1 ~ 5%, different ethylhexyl palmitate 1 ~ 2%, polydimethylsiloxane 1 ~ 5%, butylated hydroxytoluene 0.01 ~ 0.05%, propyl hydroxybenzoate 0.01 ~ 0.05%, cetearyl alcohol 1 ~ 5%, glyceryl stearate 0.5 ~ 1%, PEG-100 stearate (polyoxyethylene 100 stearate) 0.5 ~ 1%, sucrose stearate 0.3 ~ 0.6%, cetearyl glucoside 0.3 ~ 0.6%, spermol 0.4 ~ 0.8%, vitamin E 0.1 ~ 0.3%, Camellia oil 0.1 ~ 1%,
Component E: hydrolysis gadid hide collagen 1 ~ 2%, beet root extract 1 ~ 2%, carboxymethylchitosan 1 ~ 2%, antiseptic 0.1 ~ 0.6%, essence 0.1 ~ 1%.
The speed of step (1) and the stirring described in step (3) is preferably 300 ~ 800rpm.
5 ~ 10 minutes are preferably in 78 ~ 80 DEG C of insulated and stirred to the temperature retention time be dissolved in liquid completely described in step (3).
The condition of the homogenizing described in step (4) is preferably 1000 ~ 3000rpm homogenizing 3 ~ 5 minutes.
The condition of the homogenizing after adding pre-assigned B phase described in step (4) is preferably 1000 ~ 2000rpm homogenizing 3 ~ 5 minutes.
The condition of the homogenizing after adding pre-assigned C phase described in step (4) is preferably 1000 ~ 2000rpm homogenizing 3 ~ 5 minutes.
The time of the insulation described in step (4) is preferably 20 ~ 25 minutes.
Step (5) 1. described in the speed preferably 200 ~ 800rpm of homogenizing.
Step (5) 1. described in time of insulation be preferably 10 ~ 15 minutes.
Step (5) 2. described in the condition of homogenizing be preferably 200 ~ 800rpm homogenizing 10 ~ 20 seconds.
The described nutrition skin-protection emulsion containing oat peptide is preparing the application in cosmetics.
The present invention has following advantage and effect relative to prior art:
(1) nutrition skin-protection emulsion provided by the invention is by the optimization of constituent and consumption, stable in properties.
(2) preparation method of nutrition skin-protection emulsion provided by the invention is simple to operate, easily realizes the large production of factory.
(3) to adopt natural oat peptide, hydrolysis gadid hide collagen and beet root extract to carry out composite while reaching whitening and moistening skin effect for the nutrition skin-protection emulsion Major Nutrient skin care ingredient containing oat peptide provided by the invention, also contribute to providing skin-nourishing, play crease-resistant, antidotal effect.Its character is gentle, and safety is high, has no side effect to skin, is applicable to life-time service.
Accompanying drawing explanation
Fig. 1 is the result figure that embodiment 1 ~ 3 and comparative example 2 ~ 3 obtain product and affect fibroblasts.
Detailed description of the invention
Below in conjunction with embodiment and accompanying drawing, the present invention is described in further detail, but embodiments of the present invention are not limited thereto.
Embodiment 1
(1) formula (mass percent):
Component A: oat peptide surplus, methyl hydroxybenzoate 0.1%;
B component: water 8%, Magnesiumaluminumsilicate 0.5%;
Component C: 1,3 butylene glycol 2%, glycerol 1%, propylene glycol 2%, hyaluronate sodium 0.001%, xanthan gum 0.1%;
D component: squalane 1%, different ethylhexyl palmitate 1%, polydimethylsiloxane 1%, butylated hydroxytoluene 0.01%, propyl hydroxybenzoate 0.01%, cetearyl alcohol 1%, glyceryl stearate 0.5%, PEG-100 stearate (polyoxyethylene 100 stearate) 0.5%, sucrose stearate 0.3%, cetearyl glucoside 0.3%, spermol 0.4%, vitamin E 0.1%, Camellia oil 0.1%;
Component E: hydrolysis gadid hide collagen 1%, beet root extract 1%, carboxymethylchitosan 1%, 2-bromo-2-nitro-1,3-propylene glycol 0.1%, matcha essence 0.1%.
(2) preparation of nutrition skin-protection emulsion
1. under room temperature, Magnesiumaluminumsilicate is slowly added to the water, 300rpm mechanical agitation 5min, obtains B phase;
2. under room temperature, 1,3 butylene glycol, glycerol, propylene glycol, hyaluronate sodium and xanthan gum are mixed, infiltrate evenly, obtain C phase;
3. each composition component in D phase is joined oil phase reactor successively, be heated to 78 DEG C, being stirred to completely molten is liquid, obtains D phase;
4. oat peptide and methyl hydroxybenzoate are joined emulsifying pot, heated and stirred, add pre-assigned B phase, 1000rpm homogenizing 3 minutes, add pre-assigned C phase, 1000rpm homogenizing 3 minutes, is heated with stirring to 82 DEG C, 2000rpm homogenizing 5 minutes, after being uniformly dissolved, is incubated 20 minutes;
5. under vacuum, under the mixing speed of 200rpm, D phase slowly added in A+B+C phase, add rear mixing speed and carry to 600rpm, homogenizing 5 minutes, make system emulsifying even, 85 DEG C are incubated 10 minutes, lower the temperature after froth breaking;
6. cool to less than 45 DEG C, add each component of E phase successively, stir under vacuum, then 800rpm homogenizing 10 seconds, obtain the nutrition skin-protection emulsion containing oat peptide.Should avoid, lower than 40 DEG C of dischargings, avoiding producing too much bubble.
Embodiment 2
(1) formula (mass percent):
Component A: oat peptide surplus, methyl hydroxybenzoate 0.1%;
B component: water 8.3%, Magnesiumaluminumsilicate 1%;
Component C: 1,3 butylene glycol 2.5%, glycerol 1.8%, propylene glycol 2.5%, hyaluronate sodium 0.005%, xanthan gum 0.2%;
D component: squalane 3%, different ethylhexyl palmitate 1%, polydimethylsiloxane 5%, butylated hydroxytoluene 0.05%, propyl hydroxybenzoate 0.05%, cetearyl alcohol 3%, glyceryl stearate 0.9%, PEG-100 stearate (polyoxyethylene 100 stearate) 0.9%, sucrose stearate 0.6%, cetearyl glucoside 0.6%, spermol 0.6%, vitamin E 0.2%, Camellia oil 0.5%;
Component E: hydrolysis gadid hide collagen 1.5%, beet root extract 1.5%, carboxymethylchitosan 1.5%, methylchloroisothiazandnone and Methylisothiazolinone are each 0.15%, matcha essence 0.5%.
(2) preparation of nutrition skin-protection emulsion
1. under room temperature, Magnesiumaluminumsilicate is slowly added to the water, 300rpm mechanical agitation 5min, obtains B phase;
2. under room temperature, 1,3 butylene glycol, glycerol, propylene glycol, hyaluronate sodium and xanthan gum are mixed, infiltrate evenly, obtain C phase;
3. each composition component in D phase is joined oil phase reactor successively, be heated to 78 DEG C, being stirred to completely molten is liquid, obtains D phase;
4. oat peptide and methyl hydroxybenzoate are joined main emulsifying pot, heated and stirred, add pre-assigned B phase, 2000rpm homogenizing 3 minutes, add pre-assigned C phase, 2000rpm homogenizing 3 minutes, is heated with stirring to 82 DEG C, 3000rpm homogenizing 5 minutes, after being uniformly dissolved, is incubated 20 minutes;
5. under vacuum, under the mixing speed of 200rpm, D phase slowly added in A+B+C phase, add rear mixing speed and carry to 600rpm, homogenizing 5 minutes, make system emulsifying even, 82 DEG C are incubated 10 minutes, lower the temperature after froth breaking;
6. cool to less than 45 DEG C, add each component of E phase successively, stir under vacuum, then 500rpm homogenizing 10 seconds, obtain nutrition skin-protection emulsion.Should avoid, lower than 40 DEG C of dischargings, avoiding producing too much bubble.
Embodiment 3:
(1) formula (mass percent):
Component A: oat peptide surplus, methyl hydroxybenzoate 0.1%;
B component: water 8.5%, Magnesiumaluminumsilicate 2%;
Component C: 1,3 butylene glycol 3%, glycerol 2%, propylene glycol 3%, hyaluronate sodium 0.01%, xanthan gum 0.3%;
D component: squalane 5%, different ethylhexyl palmitate 2%, polydimethylsiloxane 3%, butylated hydroxytoluene 0.03%, propyl hydroxybenzoate 0.03%, cetearyl alcohol 5%, glyceryl stearate 1%, PEG-100 stearate (polyoxyethylene 100 stearate) 1%, sucrose stearate 0.6%, cetearyl glucoside 0.6%, spermol 0.8%, vitamin E 0.3%, Camellia oil 1%;
Component E: hydrolysis gadid hide collagen 2%, beet root extract 2%, carboxymethylchitosan 2%, methylchloroisothiazandnone and Methylisothiazolinone are each 0.3%, matcha essence 1%.
(2) preparation of nutrition skin-protection emulsion
1. under room temperature, Magnesiumaluminumsilicate is slowly added to the water, 300rpm mechanical agitation 5min, obtains B phase;
2. under room temperature, 1,3 butylene glycol, glycerol, propylene glycol, hyaluronate sodium and xanthan gum are mixed, infiltrate evenly, obtain C phase;
3. each component in D component is joined oil phase reactor successively, be heated to 78 DEG C, being stirred to completely molten is liquid, obtains D phase;
4. oat peptide and methyl hydroxybenzoate join main emulsifying pot, heated and stirred, add pre-assigned B phase, 1500rpm homogenizing 3 minutes, add pre-assigned C phase, 1500rpm homogenizing 3 minutes, is heated with stirring to 82 DEG C, 1000rpm homogenizing 5 minutes, after being uniformly dissolved, is incubated 20 minutes;
5. under vacuum, under the mixing speed of 200rpm, D phase slowly added in A+B+C phase, add rear mixing speed and carry to 600rpm, homogenizing 5 minutes, make system emulsifying even, 83 DEG C are incubated 10 minutes, lower the temperature after froth breaking;
6. cool to less than 45 DEG C, add each component of E phase successively, stir under vacuum, then 200rpm homogenizing 10 seconds, discharging, obtained nutrition skin-protection emulsion.Should avoid, lower than 40 DEG C of dischargings, avoiding producing too much bubble.
Comparative example 1
(1) formula (mass percent):
Component A: oat peptide surplus, methyl hydroxybenzoate 0.1%;
B component: water 8.5%, Magnesiumaluminumsilicate 2%;
Component C: 1,3 butylene glycol 3%, glycerol 2%, propylene glycol 3%, hyaluronate sodium 0.01%, xanthan gum 0.3%;
D component: squalane 6%, different ethylhexyl palmitate 4%, polydimethylsiloxane 6%, butylated hydroxytoluene 0.03%, propyl hydroxybenzoate 0.05%, cetearyl alcohol 2%, glyceryl stearate 1%, PEG-100 stearate (polyoxyethylene 100 stearate) 0.5%, sucrose stearate 0.3%, cetearyl glucoside 0.6%, spermol 0.4%, vitamin E2 %, Camellia oil 5%;
Component E: hydrolysis gadid hide collagen 2%, beet root extract 2%, carboxymethylchitosan 2%, methylchloroisothiazandnone and Methylisothiazolinone 0.3%, matcha essence 1%.
(2) preparation of nutrition skin-protection emulsion
1. under room temperature, Magnesiumaluminumsilicate is slowly added to the water, 300rpm mechanical agitation 5min, obtains B phase;
2. under room temperature, 1,3 butylene glycol, glycerol, propylene glycol, hyaluronate sodium and xanthan gum are mixed, infiltrate evenly, obtain C phase;
3. each component in D component is joined oil phase reactor successively, be heated to 78 DEG C, being stirred to completely molten is liquid, obtains D phase;
4. oat peptide and methyl hydroxybenzoate join main emulsifying pot, heated and stirred, add pre-assigned B phase, 1500rpm homogenizing 3 minutes, add pre-assigned C phase, 1500rpm homogenizing 3 minutes, is heated with stirring to 82 DEG C, 1000rpm homogenizing 5 minutes, after being uniformly dissolved, is incubated 20 minutes;
5. under vacuum, under the mixing speed of 200rpm, D phase slowly added in A+B+C phase, add rear mixing speed and carry to 600rpm, homogenizing 5 minutes, make system emulsifying even, 83 DEG C are incubated 10 minutes, lower the temperature after froth breaking;
6. cool to less than 45 DEG C, add each component of E phase successively, stir under vacuum, then 200rpm homogenizing 10 seconds, discharging, obtained nutrition skin-protection emulsion.Should avoid, lower than 40 DEG C of dischargings, avoiding producing too much bubble.
Comparative example 2, containing oat peptide, does not contain the example of hydrolysis gadid hide collagen and beet root extract
Formula for a product and preparation process are substantially with embodiment 1, and difference is only, not containing hydrolysis gadid hide collagen and beet root extract, to replace the amount of hydrolysis gadid hide collagen and beet root extract with water.
Comparative example 3, containing hydrolysis gadid hide collagen and beet root extract, does not contain the example of oat peptide
Formula for a product and preparation process are substantially with embodiment 1, and difference is only, not containing oat peptide, to replace the amount of oat peptide with water.
Physical and chemical index and efficacy test
One, the physical and chemical index test of embodiment 1 ~ 3 and comparative example 1 ~ 3 is as follows:
(1) character
The made nutrition skin-protection emulsion of the present invention is creamy white, and denseness is suitable for, and good stability, fragrant, the emulsion prepared by comparative example 1 is creamy white, and denseness is alkene comparatively, and stability is bad, has special odor.
(2) Detection of Stability
Heat resistant test: electro-heating standing-temperature cultivator 24h sample being put into (40 ± 1) DEG C, observes phenomenons such as whether having thinning, variable color, layering and firmness change, with the heat resistance of judgement sample after recovering room temperature.
Low temperature resistant test: sample is put into (-5 ~-10) DEG C ± electric refrigerator 24h of 1 DEG C, observes phenomenons such as whether having thinning, variable color, layering and firmness change, with the cold tolerance of judgement sample after recovering room temperature.
Centrifugal test: sample is placed in centrifuge, with the speed test 30min of (2000 ~ 4000) r/min, observes the separation of sample, point layer status.
Table 1 Detection of Stability result
Two, efficacy assessments test (embodiment 1 ~ 3 and comparative example 2 ~ 3)
(1) whitening test: restraint of tyrosinase active testing.
Skin-whitening agents generates by acting on dermal melanin exactly, and in the process of metabolism, check melanin generates and meets the material of safety standard.Dopa and Dopaquinone two processes are converted into because tryrosinase acts on tyrosine respectively, current skin-whitening agents generates melanic oxidative pathway by restraint of tyrosinase activity or blocking-up tyrosine exactly, thus reduces the effect that melanic generation reaches skin whitening.By judging the whitening effect of embodiment 1 ~ 3 and comparative example 2 ~ 3 to the suppression degree of tyrosinase activity.
Product configuration 5% aqueous solution (5ml product water is settled to 100mL) embodiment 1 ~ 3 and comparative example 2 ~ 3 obtained, with the arbutin of 5% concentration for positive controls (5ml product water is settled to 100mL).Enzyme digestion reaction substrate TYR is dissolved in the phosphate buffered solution of pH6.8, be mixed with the TYR solution that concentration is 1.0mmol/L, being added by the TYR solution of 1mL is numbered in 13 test tubes of A1 ~ A7 and A2 ' ~ A7 ' number, 1mL phosphate buffered solution is added in A1 test tube, in A2 ~ A7 and A2 ' ~ A7 ' number test tube, add that 1mL prepared respectively containing in the solution of the embodiment 1 ~ 3 of same concentrations and the arbutin of comparative example 2 ~ 3 and 5% concentration, then add at A2 ' ~ A7 ' number test tube the tyrosinase solution (being dissolved in pH6.8 phosphate buffered solution) that 0.5mL concentration is 173U/mL, by pH6.8 phosphate buffered solution, invisible spectro liquor capacity is mended to 3mL, in system, the final concentration of enzyme is 173U/mL.A1 ~ A7 and A2 ' ~ A7 ' number test tube is surveyed its light absorption value at 475nm place after room temperature (25 DEG C) lucifuge places 30min, is designated as A1 ~ A7 and A2 ' ~ A7 ', refers to table 2.
Be calculated as follows the suppression ratio of tyrosinase activity:
Suppression ratio (%)=[1-(A
x'-A
x)/A1] × 100.
Table 2. restraint of tyrosinase active testing
The embodiment 1 ~ 3 of same concentrations and comparative example 2 ~ 3 pairs of tryrosinases have obvious inhibitory action as seen from the results in Table 2, and the embodiment 1 ~ 3 of same concentrations is close with the inhibitory action of positive controls arbutin to tryrosinase; Embodiment 1 ~ 3 is more remarkable than the inhibitory action of comparative example 2 ~ 3 pairs of tryrosinases, illustrates that natural oat peptide, hydrolysis gadid hide collagen and beet root extract play coordinate repression to tryrosinase.
2, on fibroblasts impact test
Fibroblast participates in synthesis and secretion collagen protein, elastin laminin, the netted fiber of generation collagen fiber and elastic fiber, also synthesize and secrete glycosaminoglycan and sugared egg, with advancing age, it is that fibroblast quantity reduces, synthesis capability declines that skin aging highlights clinical manifestation, and detection embodiment 1 ~ 3 and comparative example 2 ~ 3 are on the anti-aging effects of the impact reaction embodiment emulsion of fibroblastic activity.
Adopt CCK-8 cytoactive detection method to detect the emulsion obtained in embodiment 1 ~ 3 and comparative example 2 ~ 3 to affect, using short epidermal growth factor (EGF5000IU) as positive controls human fibroblasts proliferation activity;
Method: human skin fibroblast (HSAS1, U.S. ATCC) is prepared single cell suspension, with 5 × 10
3individual cell is inoculated in 96 well culture plates, hatch 24h for 37 DEG C, often group adds embodiment 1 ~ 3 emulsion and comparative example 2 ~ 3 emulsion of 0.02% concentration, emulsion uses culture medium dilution according to mass percent, positive controls arranges 1 group, add the EGF (content is 95% for epidermal growth factor, Gibco company) of 0.02% concentration.The same with embodiment emulsion, separately establish 1 group of cellar culture matched group (not adding any tester).Often group arranges 6 parallel holes, adds that sample is follow-up continuous hatches 24h, after add CCK-815 μ l continuation cultivate 3h, 450nm wavelength and measure light absorption value, cytoactive the results are shown in Figure 1.Experimental result shows that embodiment 1 ~ 3 and comparative example 2 ~ 3 pairs of fibroblasts have facilitation, embodiment 1 ~ 3 emulsion promotes that the EGF effect of fibroblasts effect and same concentrations is close, and comparative example 2 ~ 3 promotes that the effect of fibroblastic activity is obviously without embodiment 1 ~ the last 3, illustrates that natural oat peptide, hydrolysis gadid hide collagen and beet root extract have collaborative accretion to fibroblastic activity.
(3) human body application testing
1) test method: volunteer 90 people (age 25-40 year), 6 groups are divided into immediately by the principle of parallel control, use the emulsion of blank group of (not adding the emulsion of natural oat peptide, hydrolysis gadid hide collagen and beet root extract) embodiment 1 ~ 3 and comparative example 2 ~ 3 respectively, respectively get 0.8 ~ 1.2g after clean of test volunteer morning and evening and be applied in face, use 1 month.
2) instrument: skin moisture measuring instrument, skin elasticity measuring instrument, wrinkle of skin measuring instrument that German COURAGE+KHAZAKA (CK) company produces.
3) result of the test:
Use after 1 month, the tender property of skin beauty water, elasticity are improved degree and are referred to table 3.Table 3 uses 1 month skin weekly to improve degree statistical table for volunteer, and in table, evaluation index comprises skin and slightly improves and improve two grades with moderate, and the number that its mild or moderate improves may comprise the number that moderate is improved, and number accumulates superposition in time.Slight improvement refers to moisture of skin recruitment < 5% compared with (0 week) before testing, melanin reduction < 5%, and elasticity improves degree < 5%; Moderate improves improvement degree >=5% of more than two in three that refer in moisture of skin, melanin and elasticity.
Table 3. clinical practice 1 month skin improves Tachistoscope result
Shown by table 3 result: the clinical application effect of embodiment 1 ~ 3 on skin of face is better than blank group and comparative example 2 ~ 3, it can thus be appreciated that with the addition of natural oat peptide, hydrolysis gadid hide collagen is better than the improvement degree of two kinds of effects to the skin of face of only adding in three kinds with the emulsion of beet root extract, illustrate that the conbined usage of three kinds of raw materials serves and collaborative improve the tender property of skin water, elastic effect.
Above-described embodiment is the present invention's preferably embodiment; but embodiments of the present invention are not restricted to the described embodiments; change, the modification done under other any does not deviate from spirit of the present invention and principle, substitute, combine, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (10)
1., containing a nutrition skin-protection emulsion for oat peptide, it is characterized in that being grouped into by following one-tenth by mass percentage:
Component A: oat peptide 50 ~ 77%, methyl hydroxybenzoate 0.05 ~ 0.1%;
B component: water 8 ~ 8.5%, Magnesiumaluminumsilicate 0.5 ~ 2%;
Component C: butanediol 2 ~ 3%, glycerol 1 ~ 2%, propylene glycol 2 ~ 3%, hyaluronate sodium 0.001 ~ 0.01%, xanthan gum 0.1 ~ 0.3%;
D component: squalane 1 ~ 5%, different ethylhexyl palmitate 1 ~ 2%, polydimethylsiloxane 1 ~ 5%, butylated hydroxytoluene 0.01 ~ 0.05%, propyl hydroxybenzoate 0.01 ~ 0.05%, cetearyl alcohol 1 ~ 5%, glyceryl stearate 0.5 ~ 1%, PEG-100 stearate 0.5 ~ 1%, sucrose stearate 0.3 ~ 0.6%, cetearyl glucoside 0.3 ~ 0.6%, spermol 0.4 ~ 0.8%, vitamin E 0.1 ~ 0.3%, Camellia oil 0.1 ~ 1%;
Component E: hydrolysis gadid hide collagen 1 ~ 2%, beet root extract 1 ~ 2%, carboxymethylchitosan 1 ~ 2%, antiseptic 0.1 ~ 0.6%, essence 0.1 ~ 1%.
2. according to claim 1 containing the nutrition skin-protection emulsion of oat peptide, it is characterized in that: described antiseptic is one in 2-bromo-2-nitro-1,3-propylene glycol, methylchloroisothiazandnone and Methylisothiazolinone or at least two kinds.
3. contain the preparation method of the nutrition skin-protection emulsion of oat peptide described in claim 1 or 2, it is characterized in that comprising the steps:
(1) prewired B phase: be added to the water by Magnesiumaluminumsilicate, is stirred to Magnesiumaluminumsilicate complete swelling even, obtains B phase, for subsequent use;
(2) prewired C phase: the mixing of butanediol, glycerol, propylene glycol, hyaluronate sodium and xanthan gum is infiltrated evenly, obtains C phase, for subsequent use;
(3) prewired D phase: squalane, different ethylhexyl palmitate, polydimethylsiloxane, butylated hydroxytoluene, propyl hydroxybenzoate, cetearyl alcohol, glyceryl stearate, PEG-100 stearate, sucrose stearate, cetearyl glucoside, spermol, vitamin E and Camellia oil are added oil phase reactor successively, stir while be heated to 78 ~ 80 DEG C, then in 78 ~ 80 DEG C of insulated and stirred to being dissolved into liquid completely, obtain D phase, be incubated for subsequent use;
(4) preformulation A+B+C phase: add oat peptide and methyl hydroxybenzoate in emulsifying pot, agitating heating, adds pre-assigned B phase, homogenizing; Add pre-assigned C phase, homogenizing; Be heated to 82 ~ 85 DEG C, homogenizing is to being uniformly dissolved completely, and insulation, obtains A+B+C phase, for subsequent use;
(5) preparation of nutrition skin-protection emulsion:
1. under vacuum, be added to by D in A+B+C phase, add rear homogenizing even to complete emulsifying, insulation, lowers the temperature after froth breaking;
2. be cooled to less than 45 DEG C, add hydrolysis gadid hide collagen, beet root extract, carboxymethylchitosan, antiseptic and essence successively, after stirring under vacuum, homogenizing, obtains the nutrition skin-protection emulsion containing oat peptide.
4., according to claim 3 containing the preparation method of the nutrition skin-protection emulsion of oat peptide, it is characterized in that: the speed of step (1) and the stirring described in step (3) is 300 ~ 800rpm.
5., according to claim 3 containing the preparation method of nutrition skin-protection emulsion of oat peptide, it is characterized in that: described in step (3) in 78 ~ 80 DEG C of insulated and stirred to the temperature retention time be dissolved in liquid be completely 5 ~ 10 minutes.
6., according to claim 3 containing the preparation method of the nutrition skin-protection emulsion of oat peptide, it is characterized in that: the condition of the homogenizing described in step (4) is 1000 ~ 3000rpm homogenizing 3 ~ 5 minutes.
7., according to claim 3 containing the preparation method of the nutrition skin-protection emulsion of oat peptide, it is characterized in that:
The condition of the homogenizing after adding pre-assigned B phase described in step (4) is 1000 ~ 2000rpm homogenizing 3 ~ 5 minutes;
The condition of the homogenizing after adding pre-assigned C phase described in step (4) is 1000 ~ 2000rpm homogenizing 3 ~ 5 minutes.
8., according to claim 3 containing the preparation method of the nutrition skin-protection emulsion of oat peptide, it is characterized in that:
The time of the insulation described in step (4) is 20 ~ 25 minutes;
Step (5) 1. described in time of insulation be 10 ~ 15 minutes.
9., according to claim 3 containing the preparation method of the nutrition skin-protection emulsion of oat peptide, it is characterized in that:
Step (5) 1. described in the speed of homogenizing be 200 ~ 800rpm;
Step (5) 2. described in the condition of homogenizing be 200 ~ 800rpm homogenizing 10 ~ 20 seconds.
10. the nutrition skin-protection emulsion containing oat peptide described in claim 1 or 2 is preparing the application in cosmetics.
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