CN115813774A - Water-oil double-layer refined composition with whitening effect and preparation method and application thereof - Google Patents
Water-oil double-layer refined composition with whitening effect and preparation method and application thereof Download PDFInfo
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- CN115813774A CN115813774A CN202211574442.8A CN202211574442A CN115813774A CN 115813774 A CN115813774 A CN 115813774A CN 202211574442 A CN202211574442 A CN 202211574442A CN 115813774 A CN115813774 A CN 115813774A
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- oil
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- fermentation
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Abstract
The invention relates to a water-oil double-layer refined composition with a whitening effect, and a preparation method and application thereof, and belongs to the technical field of daily chemicals. The water-oil double-layer refined composition consists of a water phase liquid and an oil phase liquid, wherein the weight ratio of the water phase liquid to the oil phase liquid is 20-70:30-80 parts; the aqueous phase liquid comprises yeast/rice fermentation product filtrate, yeast lysate extract, humectant and effective components; the oil phase liquid comprises fermented oil, and the fermented oil is obtained by fermenting the glabrous greenbrier rhizome extract, the pistachio seed oil, the glucose and the rapeseed methyl oleate together by using candida fagopyrum; the fermented oil contains a fermentation product of candida beehives, glucose and rapeseed methyl oleate, pistachio seed oil and a glycyrrhiza glabra root extract. The composition disclosed by the invention has good moisture retention and permeability, can solve the problem of greasiness in the use feeling of pure oil phase essential oil, and is fresh and cool in use feeling and not greasy.
Description
Technical Field
The invention belongs to the technical field of daily chemicals, and particularly relates to a water-oil double-layer refined composition with a whitening effect, and a preparation method and application thereof.
Background
The water-oil double-layer essential oil is popular in the market at present, and is prepared by adding water into essential oil taking a conventional oil-phase composition as a main component, so that the water-oil double-layer essential oil can effectively supplement skin oil and can also supplement water, is suitable for people with damaged skin protection barriers, and is used for regulating water-oil balance. However, the water phase liquid in the common water-oil double-layer essential oil only uses pure water, so that water is difficult to enter the skin, and the effect of replenishing water is achieved.
Disclosure of Invention
The invention aims to provide a water-oil double-layer refined composition with a whitening effect and a preparation method and application thereof.
The purpose of the invention can be realized by the following technical scheme:
the invention provides a water-oil double-layer refined composition with a whitening effect, which consists of a water phase liquid and an oil phase liquid, wherein the weight ratio of the water phase liquid to the oil phase liquid is 20-70:30-80 parts of;
the aqueous phase liquid comprises yeast/rice fermentation product filtrate, yeast lysate extract, humectant and effective components;
the oil phase liquid comprises fermented oil, and the fermented oil is obtained by fermenting pistachio (PISTACIA VERA) seed oil, glucose, rapeseed methyl oleate and a Glycyrrhiza glabra root extract together by using Candida beehives; the fermentation oil contains a Candida beehives/glucose/rapeseed methyl oleate fermentation product, pistachio seed oil and a Glycyrrhiza glabra root extract, and the Candida beehives/glucose/rapeseed methyl oleate fermentation product and the Glycyrrhiza glabra root extract are dissolved in the pistachio seed oil.
In one embodiment of the present invention, the method for preparing the fermented oil comprises the steps of:
adding the pistachio seed oil, the rapeseed methyl oleate and the glucose which are used as fermentation substrates into a fermentation tank;
inoculating the candida utilis into a fermentation substrate, controlling the temperature of the fermentation substrate to be 20-40 ℃, stirring, and fermenting for 24-48 hours to obtain fermentation liquor;
adding Glycyrrhiza glabra extract, and fermenting again;
centrifugally collecting the upper grease of the fermentation liquor;
the fermented oil is obtained by filtration and purification.
In the preparation process of the fermented oil, a twice fermentation process is adopted, and the first fermentation is mainly used for generating a fermentation product of candida utilis/glucose/rapeseed methyl oleate; the second fermentation is mainly to dissolve the fermentation product of Candida wasabi/glucose/rapeseed methyl oleate and the Glycyrrhiza glabra root extract in the pistachio seed oil. By adopting the twice fermentation process, the fermented oil forms a reverse micelle system, so that the stability of the active ingredients is improved, and the skin permeability of the active ingredients is also improved.
In one embodiment of the invention, the weight ratio of the fermentation product of the candida beehives/glucose/rapeseed methyl oleate to the extracts of the pistachio seed oil and the glycyrrhiza glabra root in the fermented oil is 0.5-2.
In one embodiment of the invention, in the fermented oil, the weight ratio of the fermentation product of candida beehives/glucose/rapeseed methyl oleate to the extracts of pistachio seed oil and glycyrrhiza glabra root is 1.
In one embodiment of the present invention, the yeast/rice fermentation product filtrate, the yeast lysate extract, the humectant and the functional components in the aqueous phase have a weight ratio relationship of 10-20:5-15:10-20:1-5.
In one embodiment of the invention, the humectant is selected from one or a combination of glycerin, butanediol, pentanediol, disodium EDTA, sodium hyaluronate crosspolymer, sodium hyaluronate, and acetylated sodium hyaluronate.
In one embodiment of the invention, the humectant is a combination of glycerin, butylene glycol, pentylene glycol, disodium EDTA, a sodium hyaluronate crosspolymer, sodium hyaluronate, acetylated sodium hyaluronate.
In one embodiment of the invention, the functional ingredient is selected from one or more of dipotassium glycyrrhizinate, ethylhexyl glycerol, xanthan gum, hydroxymethyl cellulose, arginine, trehalose, hexylene glycol and sodium lactate.
In one embodiment of the invention, the efficacy ingredient further comprises a preservative, and the preservative can be selected from phenoxyethanol.
In one embodiment of the present invention, the functional ingredient is selected from the group consisting of dipotassium glycyrrhizinate, ethylhexylglycerin, xanthan gum, hydroxymethyl cellulose, arginine, trehalose, hexylene glycol, and sodium lactate.
In one embodiment of the invention, the oil phase liquid comprises squalane, vegetable oil and fermentation oil besides the fermentation oil, and the weight ratio of the squalane, the vegetable oil and the fermentation oil in the oil phase liquid is 10-20:10-40:10-30. Because different oil formulas have different hydrophilic and oleophilic values and different surface tensions, the properties of the obtained liquid are different. Meanwhile, as the vegetable oil, the fermented oil and the squalane contain natural components which can be dissolved in a water phase, and the appearance expressions of different proportions are different, the weight proportion of the squalane, the vegetable oil and the fermented oil in the oil phase liquid is 10-20:10-40:10-30.
In one embodiment of the invention, the vegetable oil is selected from one or a combination of sunflower (helioanthus ANNUUS) seed oil, olive (OLEA EUROPAEA) fruit oil, corn germ oil.
In one embodiment of the invention, the vegetable oil is selected from the group consisting of sunflower (helioanthus ANNUUS) seed oil, olive (OLEA EUROPAEA) fruit oil, corn germ oil.
Because different vegetable oils have different fatty acid ratios, different skin feel experiences are brought, different active ingredients are contained, and the reasonable ratios of the different vegetable oils are optimized through research to achieve better skin moistening and moisturizing effects.
In one embodiment of the present invention, vegetable oil can also be used as a fermentation substrate for preparing the fermentation oil, i.e., vegetable oil, pistachio seed oil, rapeseed methyl oleate and glucose are used as the fermentation substrate together.
In one embodiment of the present invention, in the fermented water-oil two-layer composition, the weight ratio of the water phase liquid and the oil phase liquid is 20-70:30-80. Because the fermentation oil lets oil self-carried amphipathy through fermentation process, simpler vegetable oil can form specific micelle with the aqueous phase, this application can help reducing the greasy feeling in the use through the mixture of aqueous phase liquid and oily phase liquid, and the skin-friendly of oil phase more easily permeates the skin nexine simultaneously.
The invention further provides a preparation method of the fermented water-oil double-layer composition, which comprises the following steps:
preparing an aqueous phase liquid: mixing yeast/rice fermentation product filtrate, yeast lysate extract, humectant and effective components, and stirring to obtain water phase solution;
preparing an oil phase liquid: mixing squalane, vegetable oil and fermentation oil components to obtain an oil phase liquid;
and mixing the water phase liquid and the oil phase liquid according to a designed proportion to obtain the fermented water-oil double-layer composition.
In the preparation method of the fermented water-oil two-layer composition, the method of mixing the components constituting the aqueous liquid may employ a conventional stirring dissolution method.
In the preparation method of the fermented water-oil two-layer composition, the method of mixing the components constituting the oil phase liquid may employ a conventional stirring dissolution method.
The present invention further provides use of the fermented water-oil bilayer composition as a cosmetic, such as essential oil, emulsion, etc.
In the present invention, the functions of some main components are described as follows:
in the invention, the yeast/rice fermentation product filtrate is prepared by naturally fermenting rice with natural yeast, and is particularly rich in ketoglutaric acid and various active ingredients required by skin, such as oligosaccharide, organic acid, polypeptide, amino acid and the like. The probiotic bacteria can be simply and quickly absorbed and utilized by the metabolic activity of common skin probiotics (such as lactobacillus pentosus, micrococcus crusus and the like) and not absorbed and utilized by common skin pathogenic bacteria (such as propionibacterium acnes and staphylococcus aureus), so that the healthy growth of the probiotic bacteria in the skin is facilitated, the growth and the propagation of the pathogenic bacteria are competitively inhibited, and a good probiotic bacteria ecological environment beneficial to the skin is established; meanwhile, the skin care product has the effects of quickly activating skin cells, inhibiting skin inflammation and strengthening a skin cutin protection barrier.
Wherein the yeast/rice fermentation product filtrate is prepared by the following method:
(1) Adding water into rice powder, heating, stirring to obtain rice slurry, adding beta-glucanase into the rice slurry, wherein the addition amount of the beta-glucanase is 0.001-0.5% of the mass of the rice slurry, and the enzyme activity is 150-200U/g, and performing enzymolysis to obtain rice slurry enzymatic hydrolysate;
(2) Inoculating yeast strain Saccharomyces veronae into the rice pulp enzymolysis liquid, wherein the fermentation conditions are as follows: fermenting at 20-40 deg.C for 24-48 hr, and controlling pH to 6-6.5 to obtain fermentation liquid;
(3) The fermentation liquor is concentrated by a reverse osmosis technology, active substances are efficiently enriched, and rice fermentation liquor, namely yeast/rice fermentation product filtrate containing the active substances, is obtained by heating, sterilizing, cooling and filtering.
In the present invention, the yeast lysate extract can have various effects, such as improving skin 5 big problems: dull and dull, pigmentation, roughness and dryness, uneven water and oil, and loose fine lines.
Specific effects of yeast lysate extract include:
1. fully supplying cell nutrients: the extract of yeast lysate is rich in various nutrient substances, and the high proportion of amino acids, nucleosides, nucleotides, peptides, active enzymes, amino acid precursors, sterols, trace elements, vitamin B families and the like in the lysate are basically active substances which can be directly utilized, so that basic nutrition is fully provided for skin cells, and the skin cells are helped to be healthy and active.
2. Lightening melanin, whitening skin color: scientists research shows that the effect of inhibiting the formation of melanin of the melanocyte added with the live yeast is more than 60 percent compared with the melanocyte without the live yeast. It was confirmed that the live yeast had excellent whitening effect.
3. Enhancing the respiration of cells: the life and the division times of skin cells are limited, and when the skin cells approach the limit of the division times, the skin cells gradually become in a state of 'qi weakness'. The yeast lysate extract can enhance cellular respiration, and the more efficient the cells consume oxygen, the less destructive reactive oxygen Radicals (ROS) are formed.
4. Soothing skin/promoting repair: the yeast lysate extract can obviously relieve the discomfort such as reddening stabbing pain caused by skin inflammatory reaction, particularly the reddish heat sensation of the skin after mild sunburn. Meanwhile, the capability of accelerating the healing and repairing of the tiny wound of the epidermis is realized. Helps the skin to be more secure and stable.
5. Antioxidant/collagen synthesis promotion: the yeast lysate contains a plurality of accessory enzyme components, has the effect of reducing the oxidative damage of free radicals, and can improve the formation expression of skin fibroblasts, promote the synthesis of type I collagen and prevent skin relaxation and fine wrinkles.
In the invention, the squalane is similar to squalene which is an important component constituting the sebum of a human body, is a hydrogenation product of the squalene, has skin-friendly, moisturizing and antioxidant effects, is similar to the sebum component, is easier to be blended into the skin, and forms a protective film on the surface of the skin to play a barrier role.
In the invention, the fermentation product of the candida beehives/glucose/rapeseed methyl oleate has the following functions: the hydrophilic-lipophilic balance vegetable oil fermentation product has different modification degrees of grease due to a special secondary fermentation process, so that the species and the performance of active substances in the candida beehives/glucose/rapeseed methyl oleate fermentation product are more diverse, and the special structure of the fermentation product causes the fermentation product to have certain emulsifying capacity and lower surface tension compared with the unfermented vegetable oil. Can break the interface existing in the space between keratinocytes (proteins) and rapidly penetrate into the deep layers of the skin. Is more hydrophilic and is tightly combined with the moisture of the horny layer to prevent the moisture from losing. The main functions are emollient, antibacterial agent, oil control and anti-seborrhea.
In the invention, the pistachio seed oil contains more tannins, anthocyanin galactoside, anthocyanin glucoside, lutein and the like. It has effects in scavenging free radicals, and can be used as antioxidant to prevent aging; has antibacterial effect on Escherichia coli, staphylococcus aureus, and Streptococcus albus, and can be used as antiinflammatory agent. In the invention, the pistachio seed oil is used as a fermentation product, and the biological stability and activity of the glycyrrhiza glabra root extract can be improved.
In the invention, the glycyrrhiza glabra extract can penetrate into the skin and keep high activity, effectively inhibits the activity of various enzymes (such as tyrosinase) in the melanin generation process, and is a quick, efficient, safe and green sun-screening, whitening and freckle-removing cosmetic additive. Meanwhile, the glycyrrhiza glabra extract also has the effects of preventing rough skin, resisting inflammation and resisting bacteria. In the invention, the glycyrrhiza glabra extract is dissolved in the pistachio seed oil in the fermentation process, so that the biological stability and the activity of the glycyrrhiza glabra root extract are improved.
In the application, pistachio (PISTACIA VERA) seed oil, rapeseed methyl oleate and glucose are used as fermentation substrates together, a unique fermentation oil preparation process is adopted, oleophilic bacteria are selected as fermentation strains, fermentation oil is obtained through unique biological fermentation technology, and the fermentation oil takes candida/glucose/rapeseed methyl oleate fermentation products and pistachio (PISTACIA VERA) seed oil as main components, is rich in rich skin-nourishing components, has excellent antioxidant effect and skin-friendly performance, can brighten skin color, tenders skin and resists the aging of a sebum barrier.
According to the scheme, the yeast/rice fermentation product filtrate and the fermentation oil are effectively combined to obtain the composition. The fermented oil and the yeast/rice fermentation product filtrate have good permeability, can help skin maintain microecological balance state, keep moisture and relieve, and has whitening effect. The composition has oil-like moisturizing effect and is water-refreshing.
The scheme of the application mainly adopts an anhydrous formula, and the formula uses fermentation liquor as a water phase without adding extra water. Simultaneously, the pistachio (PISTACIA VERA) seed oil, the glucose, the rapeseed methyl oleate and the Glycyrrhiza glabra root extract are fermented together by using the Candida beehives to obtain the fermented oil, and the fermented oil contains the fermentation product of the Candida beehives/the glucose/the rapeseed methyl oleate, the Pistachio (PISTACIAVERA) seed oil and the Glycyrrhiza glabra root extract. Through deep secondary fermentation, the glycyrrhiza glabra extract is better dissolved into an oil phase, and simultaneously, the active ingredients are better exerted.
The microbial fermentation filtrate is used for replacing water in the original water phase, and no additional water is added in the whole formula. The yeast/rice fermentation product filtrate is rich in ketoglutaric acid, and has good permeability, deep water supplementing, and antiaging effects.
Compared with the prior art, the invention has the advantages that:
in the invention, the yeast/rice fermentation product filtrate is used as a main component of the aqueous phase liquid instead of water, and is compounded with other functional components, and no additional water is added in the formula. Meanwhile, the fermentation oil is used as the main component of the oil phase liquid. And the water phase liquid and the oil phase liquid are arranged in a special proportion, so that the moisturizing and permeability are good, the problem of greasiness in the use experience of the pure oil phase essential oil can be solved, and the use experience is fresh and not greasy.
In the invention, a special deep fermentation preparation process is adopted, and a fermentation product of the candida utilis/glucose/rapeseed methyl oleate is dissolved in pistachio (PISTACIA VERA) seed oil. Functional oil pistachio (PISTACIA VERA) seed oil is selected for deep fermentation of single oil, fermentation process conditions are controlled in the fermentation process, and biological lipid is enriched, so that the fermented oil has the oil-water bridge characteristic, and the stability and the efficacy of the water and the oil are improved. Meanwhile, the fermentation oil brought by deep fermentation can form a self-assembly system to wrap the effective components to form micelles.
According to the invention, when the double-layer water-oil mixture is mixed after shaking, the water-oil characteristics of the fermented oil can wrap the effective components and help the effective components to better enter the skin.
In the invention, the fermented oil and the rice fermented filtrate have good permeability, help the skin to maintain a microecological balance state, keep moisture and relieve, and have a whitening effect.
In the invention, the formula design conforms to the technical definition of natural ingredients, and the index of the natural ingredients can reach more than 99 percent according to ISO 16128.
Drawings
FIG. 1: using the product provided by example 1, comparative examples 1, 2, 3, 4 for post-myo-epidermal micro-ecology (bar graphs corresponding to D0 or D16 in fig. 1 from left to right for example 1, comparative example 2, comparative example 3, comparative example 4, respectively);
Detailed Description
The invention is described in detail below with reference to the figures and the specific embodiments.
Example 1
A specific fermented water-oil bilayer composition is provided, the formulation of which is shown in table 1.
Table 1 formulation of example 1
The preparation process of the fermented oil comprises the following steps:
adding pistachio (PISTACIA VERA) seed oil, rapeseed methyl oleate and glucose as fermentation substrate into a fermentation tank, and sterilizing at 100-120 deg.C for 10-20 min;
inoculating the candida albicans into a fermentation substrate sterilized at high temperature, and fermenting to obtain fermentation liquor;
adding Glycyrrhiza glabra extract, adjusting temperature to 30-60 deg.C, fermenting for 1-2 hr;
centrifugally collecting the upper grease of the fermentation liquor;
the fermented oil is obtained by filtration and purification.
In example 1, 21 parts by weight of the fermented oil contained 3 parts by weight of a fermentation product of Candida beehives/glucose/rapeseed methyl oleate and 15 parts by weight of pistachio (Pistacia VERA) seed oil, and 3 parts by weight of Glycyrrhiza glabra extract was added.
The preparation method of the yeast/rice fermentation product filtrate comprises the following steps:
(1) Weighing 30kg of brown rice, soaking the brown rice into 70kg of water for accelerating germination for 24h, drying the brown rice for 12h at 50 ℃, and crushing the brown rice into brown rice powder which is completely sieved by 200 meshes, wherein the water content is 10%; adding 70L of water to obtain rice milk, adding 1.5mmol/L of CaCl 2 A solution;
(2) Adding beta-glucanase into the rice pulp, wherein the addition amount of the beta-glucanase is 0.5 percent of the mass of the rice pulp, the enzyme activity is 150-200U/g, carrying out enzymolysis, the enzymolysis temperature is 35-40 ℃, the time is 10 hours, the enzymolysis is at the end point, and the glucose content at the interval of 1 hour at the enzymolysis end point is not changed any more, so as to obtain rice pulp enzymolysis liquid;
(3) Inoculating a yeast strain Saccharomyces veronae into the rice pulp enzymolysis liquid, wherein the fermentation conditions are as follows: fermenting at 30 deg.C for 24 hr, and controlling pH to 6 + -0.2 to obtain fermentation liquid;
(4) The fermentation liquor is subjected to a multistage reverse osmosis technology, the reverse osmosis pressure is 3.5MPa, the filtration temperature of a reverse osmosis membrane is controlled to be 35 ℃, the fermentation liquor is operated until the volume of a cut-off part is 50% of the total volume, and the fermentation liquor is concentrated to 10 times through a 5-stage osmosis process to obtain 10 times of concentrated yeast/rice fermentation liquor which is rich in active ingredients.
No water is added in the preparation process of the formula.
The preparation process of the fermented water-oil double-layer composition comprises the following steps:
1) Ensuring that production equipment is dried without water, sequentially adding the phase A raw materials into a main tank, stirring and mixing at normal temperature 100rpm for 30 minutes, and dissolving uniformly;
2) Then adding the phase B raw material into the oil pan, and uniformly stirring;
3) And mixing the water phase liquid and the oil phase liquid according to a designed proportion to obtain the fermented water-oil double-layer composition.
Comparative examples were set up and a list of specific ingredients is shown in table 2.
TABLE 2
Comparative example 1 contained no fermentation oil and yeast/rice fermentation product filtrate at the same time.
Comparative example 2 contained yeast/rice fermentation filtrate, but no fermentation oil.
Comparative example 3 contained no yeast/rice fermentation product filtrate, but fermented oil.
The fermented oil in comparative example 4 was prepared by a one-time fermentation process, i.e., pistachio seed oil, rapeseed methyl oleate, glucose, and glycyrrhiza glabra extract were used together as fermentation substrate and added to a fermentation tank; inoculating the candida utilis into a fermentation substrate, controlling the temperature of the fermentation substrate to be 20-40 ℃, stirring, and fermenting for 24-48 hours to obtain fermentation liquor; centrifugally collecting the upper grease of the fermentation liquor; the fermented oil is obtained by filtration and purification.
To verify the efficacy of the water-oil double-layer refined composition of the present invention, the following effect verification tests were performed.
Test example for Effect verification
1. Micro-ecological testing
50 women (5 groups of 10 women, using the products of example 1 and comparative examples 1-4, respectively) were selected, without problem skin, aged 30-45 years, were applied on a half-face test, and were used 2 times a day after skin cleansing for 15 days.
Regulation of microbial populations
Facial sampling for skin microbiota analysis (16S-rDNA sequence, metagenomic analysis): diversity index and bacterial make-up.
Shannon diversity index test microbial diversity: testing of the muscle surface Microecology after use of the product of example 1
And (4) conclusion: as can be seen from the data in fig. 1 (average data of 10 subjects using example 1, ordinate data in fig. 1 is shannon diversity index), there was no significant difference in D16 VS D0 of example 1 after 15 days of use, while there was a certain difference in D16 VS D0 of comparative examples 1-4, indicating that the use of the composition provided in example 1 can maintain the stability of microecological diversity. As can be seen from FIG. 1, the comparative examples and comparative examples, using the composition of example 1, can regulate the bacterial constitution and increase the composition of beneficial bacteria. Illustrating the ability of the composition of example 1 to effectively maintain the stability of bacterial diversity and maintain the microbiota of healthy skin.
Table 3 self-evaluation of subjects table (satisfaction)
And (4) conclusion: as can be seen from the data in Table 3, the subjects considered by self-evaluation mostly can improve the skin glossiness, improve the moisture degree, shrink pores, and ensure that the skin is not greasy easily, and the overall evaluation can improve the skin state integrally and reappear the delicate and good skin quality.
2. Moisture retention and soothing efficacy test
Test samples: example 1 sample example and comparative examples 1-4; the test subjects were 50, divided into 5 groups on average, and each test sample was tried.
The test method comprises the following steps: the test sample is added in the amount of (2 +/-0.1) mg/cm 2 The amount of (c) was applied in a single application, using a latex finger cot to evenly apply the sample to the inside of the left or right anterior arm, for a single application lasting 6 hours.
The results were tested at the following 4 time periods, respectively: before use of the sample (D0), after use of the sample for 1h, after use of the sample for 3h, and after use of the sample for 6 h.
The detection items are as follows:
the moisture content of stratum corneum of 5 skin was measured by a Corneometer using a C in M82.
And (3) testing results:
as shown in tables 4-5, the test results of the efficacy tests using the sample example of example 1 and the comparative examples 1-4 (the data in the table is the average value of 10 subjects in each group), the moisture content of stratum corneum of skin in the sample area was significantly increased after 1h, 3h and 6h of the sample example of example 1, and was increased by 61.82%, 49.75% and 42.16%, respectively.
TABLE 4 moisture content of the stratum corneum
TABLE 5 rate of change of moisture content of stratum corneum
3. Evaluation of whitening Effect
Test samples: example 1 sample example and comparative examples 1-4;
the test method comprises the following steps: the samples were used twice daily, morning and evening, and tried for 8 weeks. One side of the random was painted with the appropriate amount of test sample and then with sunscreen product, and the other side was painted with sunscreen product alone without test sample as a control. The sunscreen product is applied to the whole face every morning. The test subjects were 50, divided into 5 groups on average, and each test sample was tried.
The results were tested at the following 4 time periods, respectively: before use of the sample (D0), after use of the sample for 14 days (D14), after use of the sample for 28 days (D28), and after use of the sample for 56 days (D56).
The detection items are as follows:
1) Skin melanin tester
MX18 is used for the detection of skin melanin;
2) Skin color tester
CL200 is used to detect skin glossiness;
and (3) testing results:
as shown in table 6 (average of 10 subjects per group in table), the results of the efficacy test experiment of the test samples were obtained by testing melanin at the target site of the subject (measured value was decreased, indicating that the skin melanin content was smaller) 56 days after using the example of the sample of example 1, and when the example of the sample was not used, the melanin value of the skin was 48.15, which is a dimensionless amount, and the melanin value was 47.02 after 14 days, 43.06 after 28 days, and 41.29 after 56 days. The skin melanin values were significantly reduced by 14.25% after 56 days of use compared to before use. The prepared double-layer fermentation essence oil can effectively reduce the generation of skin melanin.
TABLE 6 example 1 sample example and comparative examples 1-4 skin melanin change rates after 56 days
As a result of the efficacy test experiments for the test samples shown in Table 7, the skin gloss at the target site of the subject was measured 56 days after the use of the sample (the measured value was increased, indicating that the skin gloss was better), and the skin gloss obtained without the sample of example 1 was 32.58, which is a dimensionless amount, 32.97 after 14 days, 33.02 after 28 days, and 33.05 after 56 days. The skin glossiness of the area of the sample of example 1 is increased by 1.44%, which shows that the prepared double-layer fermented essence oil can effectively improve the skin glossiness.
TABLE 7 EXAMPLE 1 sample example and comparative example the rate of change in skin gloss after 56 days
The embodiments described above are described to facilitate an understanding and use of the invention by those skilled in the art. It will be readily apparent to those skilled in the art that various modifications to these embodiments may be made, and the generic principles described herein may be applied to other embodiments without the use of the inventive faculty. Therefore, the present invention is not limited to the above embodiments, and those skilled in the art should make improvements and modifications within the scope of the present invention based on the disclosure of the present invention.
Claims (10)
1. The water-oil double-layer refined composition with the whitening effect is characterized by consisting of a water phase liquid and an oil phase liquid, wherein the weight ratio of the water phase liquid to the oil phase liquid is 20-70:30-80 parts;
the aqueous phase liquid comprises yeast/rice fermentation product filtrate, yeast lysate extract, humectant and effective components;
the oil phase liquid comprises fermented oil, and the fermented oil is obtained by fermenting pistachio seed oil, glucose, rapeseed methyl oleate and a glycyrrhiza glabra root extract together by using candida utilis; the fermented oil contains a fermentation product of candida beehives, glucose and rapeseed methyl oleate, pistachio seed oil and a glycyrrhiza glabra root extract.
2. The water-oil double-layer refined composition with whitening effect according to claim 1, wherein the preparation method of the fermented oil comprises the following steps:
adding the pistachio seed oil, the rapeseed methyl oleate and the glucose which are used as fermentation substrates into a fermentation tank;
inoculating the candida utilis into a fermentation substrate, controlling the temperature of the fermentation substrate to be 20-40 ℃, stirring, and fermenting for 24-48 hours to obtain fermentation liquor;
adding Glycyrrhiza glabra extract, and fermenting again;
centrifugally collecting the upper grease of the fermentation liquor;
the fermented oil is obtained by filtration and purification.
3. The water-oil double-layer refined composition with whitening effect according to claim 1, wherein the fermented oil contains candida faecium/glucose/rapeseed methyl oleate fermentation product, pistachio seed oil and glycyrrhiza glabra root extract in a weight ratio of 0.5-2.
4. The water-oil double-layer refined composition with whitening effect according to claim 1, wherein the water-phase liquid contains the yeast/rice fermentation product filtrate, the yeast lysate extract, the humectant and the effective components in a weight ratio relationship of 10-20:5-15:10-20:1-5.
5. The water-oil double-layer essence composition with whitening effect according to claim 1, wherein the humectant is one or more selected from glycerin, butanediol, pentanediol, disodium EDTA, sodium hyaluronate cross-linked polymer, sodium hyaluronate, and sodium acetylated hyaluronate;
the functional components are selected from one or more of dipotassium glycyrrhizinate, ethylhexyl glycerol, xanthan gum, hydroxymethyl cellulose, arginine, trehalose, hexanediol and sodium lactate.
6. The water-oil double-layer refined composition with whitening effect according to claim 1, wherein the oil phase liquid comprises squalane and vegetable oil in addition to the fermented oil, and the weight ratio of the squalane, the vegetable oil and the fermented oil in the oil phase liquid is 10-20:10-40:10-30.
7. The water-oil double-layer refined composition with whitening effect according to claim 6, wherein the vegetable oil is selected from one or more of sunflower seed oil, olive fruit oil or corn germ oil.
8. The water-oil double-layer refined composition with whitening effect according to claim 1, wherein the weight ratio of the water phase liquid to the oil phase liquid in the fermented water-oil double-layer composition is 44.
9. The water-oil double-layer refined composition with whitening effect according to claim 1, which is characterized by comprising the following components in parts by weight:
Yeast/Rice fermentation product filtrate 14
Glycerol 5
Butanediol 5.5
Pentanediol 5
EDTA disodium 0.18
Sodium hyaluronate Cross-linking Polymer 0.1
Hyaluronic acid sodium salt 0.1
Acetylated sodium hyaluronate 0.1
Yeast lysate extract 10
Dipotassium glycyrrhizinate 0.1
Ethyl hexyl Glycerol 0.5
Xanthan gum 0.1
Hydroxymethyl cellulose 0.22
0.2 parts of phenoxyethanol
Arginine 0.1
Trehalose 0.3
Hexanediol 1.5
Sodium lactate 1
Squalane 10
Sunflower seed oil 8
Olive fruit oil 7
Corn germ oil 10
21, fermentation oil;
wherein 21 parts by weight of the fermented oil contains 3 parts by weight of a fermentation product of candida beehives/glucose/rapeseed methyl oleate, 15 parts by weight of pistachio seed oil and 3 parts by weight of a glycyrrhiza glabra extract.
10. Use of the water-oil double-layer refined composition having whitening effect of any one of claims 1 to 9, wherein the water-oil double-layer refined composition having whitening effect is used as a cosmetic.
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