CN115518012B

CN115518012B - Composition with effects of inhibiting melanin protein and improving black eye and eye pouch and application thereof

Info

Publication number: CN115518012B
Application number: CN202211222635.7A
Authority: CN
Inventors: 李雄山
Original assignee: Guangzhou Archimedes Biotechnology Co ltd
Current assignee: Archimedes Guangzhou Cosmetics Research Co ltd
Priority date: 2022-10-08
Filing date: 2022-10-08
Publication date: 2023-06-09
Anticipated expiration: 2042-10-08
Also published as: CN115518012A

Abstract

The invention provides a composition with the effects of inhibiting melanin protein and improving dark circles and eye bags and application thereof. The extract can inhibit expression of kinesin, reduce melanin cell transfer, and relieve black eye symptoms; peony root extract for promoting oxygen supply recovery and cell injury repair of blood vessels around eyes and relieving problems of dark circles and eye bags; lutein and superoxide dismutase have effects of scavenging free radicals and resisting oxidation, and can rapidly scavenge damaging free radicals generated by cell metabolism, and promote recovery of cell injury around eyes. The thyme fermented extract, the peony root extract, the lutein and the superoxide dismutase complement each other, and have synergistic effect, and have the effects of stronger antioxidation, dark circle fading, skin tightening and eye bag improvement.

Description

Composition with effects of inhibiting melanin protein and improving black eye and eye pouch and application thereof

Technical Field

The invention belongs to the technical field of daily chemicals, relates to a composition and application thereof, and in particular relates to a composition with the effects of inhibiting melanin and improving black eyes and eye bags and application thereof.

Background

Under the current fast-paced life, people have high pressure and stay up night as a normal state, and in life and work, irregular work and rest and insufficient sleep can cause the blood flow velocity of skin blood vessels of eyes to be too slow to form stagnation; insufficient oxygen supply to tissues and excessive accumulation of metabolic waste in blood vessels cause pigmentation of eyes to form dark circles, and long-term stay up can cause skin relaxation of lower eyelid to form eye bags.

The black eye is mainly divided into two types: a black eye is mainly caused by insufficient oxygen supply of skin red blood cells of eyes due to night, aging, eye fatigue, etc., slow metabolism of metabolic wastes in veins and low oxygen content of venous blood retained eyes. The other is dark brown eye circles, mainly due to eye pigmentation caused by melanin generation and metabolic insufficiency. The clinical manifestations of the pouch are loose and piled-up lower eyelid skin, the orbital fat is drooped in a pouch shape, the outer canthus position moves downwards, the lower eyelid margin is not tightly attached to the eyeball, the radian of the lower eyelid margin increases, the lower punctum moves outwards to overflow tears, and the aging absorption of facial bones leads to secondary relaxation of the lower eyelid supporting structure. The skin around the eyes is thin, only a quarter of the thickness of the facial skin, and is susceptible to various damaging factors causing fat accumulation to form an eye pouch.

One of the main measures for improving the black eye is to reduce the formation and transfer of melanin, an amino acid derivative, which is formed by a series of chemical reactions of tyrosine or 3, 4-dihydroxyphenylalanine, which is often present in a polymerized form. Melanin is synthesized by melanocytes in basal layers of human epidermis, and when ultraviolet light is irradiated on the skin, the skin is in a 'self-protection' state, activates tyrosinase activity, catalyzes oxidation of tyrosine or 3, 4-dihydroxyphenylalanine to form melanin, and protects our skin cells. Melanin is also displayed on the top layer of the skin by the layer-by-layer movement of cellular metabolism. Enzymes mainly involved in melanin synthesis include tyrosinase, dopachrome tautomerase and dihydroxyindole carboxylic acid oxidase, wherein tyrosinase belongs to oxidoreductase, is a main rate-limiting enzyme for melanin synthesis, and the activity of tyrosinase is used for determining the quantity of melanin formation. Tyrosinase has the characteristics of monophenolase and diphenolase, namely has the functions of oxygenase and oxidase. During melanin synthesis, it is able to catalyze the hydroxylation of L-tyrosine to levodopa (monophenolase activity); the second step oxidizes levodopa to produce dopaquinone (diphenolase activity), which is unstable in nature and eventually produces melanin after various complex non-enzymatic reactions.

Melanin transfer is in turn associated with Kinesin, a motor protein isolated from squid axises in 1985. Kinesins are a large complex protein consisting of several distinct domains, including two heavy and two light chains, with a total molecular weight of 380kDa. It has a pair of spherical heads, a "motor" that generates power; and a fanned tail, which is a binding site for carrying protein. Inhibition of Kinesin Kinesin reduces melanin intracellular migration, which acts to alleviate melanin accumulation.

Patent document CN113730330a discloses an eye cream composition with black eye removing effect and a preparation method thereof. The black eye removing effect eye cream composition comprises the following raw material components: 0.5 to 35.0 percent of eye black-removing functional components, 5.0 to 30.0 percent of emollient, 0.5 to 30.0 percent of emulsifier, 0.5 to 20.0 percent of filler, 0.1 to 10.0 percent of thickener, 0.3 to 5.0 percent of preservative, 0.01 to 1.0 percent of daily essence and the balance of deionized water. The functional component for removing black eye of eyes is formed by mixing plankton extract, snail secretion filtrate, saccharomycete lysate extract and fucus serrulata extract. The black eye removing functional component can act on the second layer and the third layer of the skin of eyes, effectively inhibit the generation of melanin, lighten accumulated pigment, have good black eye removing effect, effectively supplement nutrition, maintain skin elasticity and compactness, and improve the unexpected appearance phenomena of the skin around eyes such as dark complexion, color spots, pouch wrinkles and the like.

Patent document CN110179715a discloses a black eye removing composition, a black eye removing eye mask and a preparation method thereof, wherein the black eye removing composition comprises the following components: flos Chrysanthemi Indici extract, herba Hedyotidis Diffusae extract, prunellae Spica extract and semen Cassiae extract. The black eye removing composition can effectively remove black eyes, restore skin vitality, and also has the effect of relieving eye fine wrinkles.

In the prior art, a plurality of plant extracts are selected to relieve the problems of dark circles and eye bags, but a plurality of effects are single, so that the composition formula is explored to achieve the multiple effects of relieving, whitening eye skin, tightening skin and restoring skin elasticity, and simultaneously, the composition has high safety, mild use and strong moisture retention.

Disclosure of Invention

Aiming at the defects of the prior art, the invention aims to provide a composition with the effects of inhibiting melanin and improving dark circles and eye bags and application thereof.

To achieve the purpose, the invention adopts the following technical scheme:

in a first aspect, the present invention provides a composition having melanin inhibiting, black eye and eye pouch improving effects, the composition comprising a thyme fermented extract, a peony root extract, lutein, and superoxide dismutase.

The thyme extract has fragrant smell, is a common spice, and can effectively inhibit the expression of kinesin and block melanin in melanocytes from being transported to the outside of the cells, so that the effect of whitening skin is achieved. The Paeonia suffruticosa root extract contains paeonol component, and paeonol has antiinflammatory, antiallergic, tranquilizing and antibacterial effects, and can be used as antibacterial agent or antiinflammatory agent for cosmetic. Lutein, an alias plant lutein, is a carotenoid with chemical formula C ₄₀ H ₅₆ O ₂ The lutein is a main pigment existing in the macula area of human eyes, is safe and harmless, has the functions of resisting oxidization and inhibiting free radicals, and is an important natural health-care product and food raw material. Superoxide dismutase is an antioxidant metalloenzyme existing in organisms, and can catalyze superoxide anion free radical disproportionation to generate oxygen and hydrogen peroxide, and plays a vital role in maintaining the balance of oxidation and antioxidation.

The invention creatively applies the combination of four components of the thymus fermented extract, the peony root extract, the lutein and the superoxide dismutase to the treatment of the dark under eye and the eye pouch, and mainly improves the problems of the dark under eye and the eye pouch from 3 aspects: 1. the fermentation extract of thyme inhibits the expression of kinesin, reduces the cell transfer of melanin, and further relieves the symptoms of dark circles; 2. the peony root extract has the effect of enhancing immunity, can play a role in repairing cell injury, further improve skin cell injury caused by external adverse factors, promote oxygen supply recovery of blood vessels around eyes and repair cell injury, and relieve the problems of dark circles and eye bags; 3. lutein and superoxide dismutase have effects of scavenging free radicals and resisting oxidation, and can rapidly scavenge damaging free radicals generated by cell metabolism, and promote recovery of cell injury around eyes. The thyme fermented extract, the peony root extract, the lutein and the superoxide dismutase complement each other, and have synergistic effect, and have the effects of stronger antioxidation, dark circle fading, skin tightening and eye bag improvement.

Preferably, the composition comprises 1-10 parts by mass of thyme fermented extract, 0.5-3 parts by mass of peony root extract, 0.01-0.5 part by mass of lutein and 0.001-0.05 part by mass of superoxide dismutase.

The mass fraction of the thyme fermented extract can be 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts or the like.

The mass fraction of the peony root extract can be 0.5 part, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts or the like.

The lutein may be 0.01 part, 0.05 part, 0.1 part, 0.2 part, 0.3 part, 0.4 part, 0.5 part, etc.

The mass fraction of the superoxide dismutase can be 0.001 part, 0.01 part, 0.02 part, 0.03 part, 0.04 part or 0.05 part, etc.

Other specific point values within the above numerical ranges are selected, and will not be described here again

Preferably, the composition further comprises a small molecule active.

Preferably, the small molecule active substance includes any one or a combination of at least two of 3-o-ethyl ascorbic acid, adenosine, carnosine, tetrapeptide-1 or caffeine, and the combination of at least two of the two includes, for example, a combination of 3-o-ethyl ascorbic acid and adenosine, a combination of adenosine and carnosine, or a combination of carnosine and tetrapeptide-1, and any other combination manner may be selected, which will not be described herein again.

Preferably, the small molecule active substance comprises any one or a combination of at least two of 1-5 parts by mass of 3-o-ethyl ascorbic acid, 0.5-5 parts by mass of adenosine, 0.5-5 parts by mass of carnosine, 0.001-0.05 parts by mass of tetrapeptide-1, or 0.1-1 parts by mass of caffeine.

The mass portion of the 3-o-ethyl ascorbic acid can be 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts or 5 parts, etc.

The adenosine may be 0.5 part, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, or the like by mass.

The mass fraction of the carnosine can be 0.5 part, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, or the like.

The tetrapeptide-1 may be 0.001 part, 0.01 part, 0.02 part, 0.03 part, 0.04 part, 0.05 part, or the like.

The caffeine may be 0.1 part, 0.2 part, 0.3 part, 0.4 part, 0.5 part, 0.6 part, 0.7 part, 0.8 part, 0.9 part, 1 part, or the like.

Other specific point values in the numerical ranges are selectable, and will not be described in detail herein.

The 3-o-ethyl ascorbic acid can inhibit the activity of tyrosinase and inhibit the generation of melanin, thereby achieving the effects of removing freckle and whitening. And 3-o-ethyl ascorbic acid has good permeability and high stability, and has hydrophilicity and lipophilicity, and is easy to enter dermis layers through stratum corneum to be absorbed by skin.

Adenosine is a compound formed by connecting N-9 of adenine and C-1 of D-ribose through beta glycosidic bond, and has a chemical formula of C ₁₀ H ₁₃ N ₅ O ₄ The phosphate is adenylate. Adenosine is a cosmetic material with good anti-wrinkle effect.

Carnosine is also called beta-alanyl-L-histidine, which is a dipeptide composed of two amino acids, beta-alanine and L-histidine, and is a crystalline solid. Muscle and brain tissue contain very high concentrations of carnosine. Carnosine has strong antioxidant capacity and can remove Reactive Oxygen Species (ROS) and alpha-beta unsaturated aldehyde formed by excessive oxidation of fatty acids of cell membranes in the oxidative stress process.

Tetrapeptide-1 is a small molecular oligopeptide, has good compatibility with skin, is easy to absorb, has anti-inflammatory and antioxidant effects, can remove wrinkles caused by muscle contraction, and also has the effects of inhibiting skin aging, improving skin elasticity and compactness, and moistening and smoothing skin.

Caffeine has a molecular formula of C ₈ H ₁₀ O ₂ N ₄ Is a white crystalline xanthine alkaloid with three methyl groups, is difficult to dissolve in cold water and is easy to dissolve in hot water. Caffeine has pharmacological activities as a central nervous system stimulant, cardiotonic, diuretic, etc., and is effective in decomposing fat, especially cellulite. Specifically, caffeine can increase the amount of cAMP by inhibiting the activity of nucleotide phosphodiesterase, thereby promoting lipolysis by enhancing the activity of triacylglycerol lipase, and can reduce accumulation of pouch fat for periocular skin, and improve pouch problems.

The composition provided by the invention is added with small molecular active substances, so that the effects of improving the problems of dark circles and under-eye bags can be further enhanced in the aspects of promoting the absorption of four active ingredients of thymus fermented extract, peony root extract, lutein and superoxide dismutase, enhancing the oxidation resistance of the composition, enhancing the lipolysis and the like.

Preferably, the composition further comprises any one or a combination of at least two of chlorella extract, witch hazel extract or lysates of fermentation products of the two yeasts, wherein the combination of at least two of the two comprises, for example, a combination of chlorella extract and witch hazel extract, a combination of witch hazel extract and lysates of fermentation products of the two yeasts, or a combination of chlorella extract and lysates of fermentation products of the two yeasts, and other arbitrary combinations are optional and will not be described in detail herein.

Further preferably, the composition further comprises a combination of chlorella extract, witch hazel extract, and lysates of fermentation products of saccharomyces cerevisiae.

The chlorella extract contains various cell active substances, forms the specific cell regeneration factors, and obviously improves the repair capability of damaged cells and DNA damage after the active ingredients in the chlorella are absorbed by skin.

The Hamamelis mollis extract contains tannin and volatile oil. Tannins, also called tannins or tannins, are complex polyphenol compounds with larger molecular weight in plants, and have strong astringency, and have the effects of resisting bacteria, regulating sebum secretion in pores, promoting blood circulation and relieving skin.

The lysate of the fermentation product of the saccharomyces cerevisiae contains rich amino acids, polypeptides, minerals and various vitamins, and has the effects of maintaining the water-oil balance of skin and repairing skin injury.

Any one or a combination of at least two of chlorella extract, witch hazel extract or yeast fermentation product lysate is further added into the composition, so that the effects of repairing skin injury, resisting bacteria and the like of the composition can be improved, and further, the effects of reducing dark circles, tightening skin and astringing eye bags are improved. When the combination of chlorella extract, witch hazel extract and yeast lysate is further selected to be added into the composition, the composition has stronger effect of improving the problems of dark under eye and under eye bags.

Preferably, the composition further comprises 0.5-5 parts by mass of chlorella extract, 0.5-5 parts by mass of witch hazel extract and 0.5-10 parts by mass of lysate of fermentation product of saccharomyces cerevisiae.

The mass fraction of the chlorella extract can be 0.5 part, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, etc.

The witch hazel extract may be 0.5 parts, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, etc.

The mass fraction of the lysate of the fermentation product of the saccharomyces cerevisiae can be 0.5 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts or 10 parts, etc.

In a second aspect, the invention provides a preparation with the effects of inhibiting melanin and improving dark circles and under-eye bags, wherein the preparation raw materials of the preparation comprise deformed vesicles and the composition with the effects of inhibiting melanin and improving dark circles and under-eye bags, which are wrapped in the deformed vesicles.

Preferably, the formulation of the formulation having the efficacy of inhibiting melanin, improving dark-eyedrops and eye bags includes an emulsion, essence, cream, gel, spray or liquid dressing.

The preparation with the effects of inhibiting melanin and improving dark circles and the eye bags contains the composition according to the first aspect, has the effects of resisting oxidization, weakening dark circles, tightening skin and improving the eye bags, and can be added with different auxiliary materials to replace different dosage forms according to actual requirements.

Preferably, the preparation raw materials of the preparation further comprise any one or a combination of at least two of butter oil, ethylhexyl palmitate or cyclopentadimethicone, and the combination of at least two of the above materials comprises, for example, a combination of butter oil and ethylhexyl palmitate, a combination of ethylhexyl palmitate and cyclopentadimethicone, or a combination of butter oil and cyclopentadimethicone, and other arbitrary combinations are optional and will not be described herein.

Preferably, the deformed vesicles are prepared from a raw material comprising polyglycerol ester surfactant, sorbitan ester surfactant, cetyl phosphate, honokiol and magnolol.

According to the invention, glycerol and natural grease are subjected to chemical synthesis to form a bilayer, so that polyglycerol esters are obtained; sorbitan ester surfactants can affect the permeability, encapsulation efficiency and rigidity of the bilayer; cetyl phosphate is beneficial to improving the stability of the bilayer; honokiol and magnolol have the property of regulating the stability, shape and size of the bilayer. In the deformed vesicle, the bilayer contains no phospholipids, contains only a single-chain surfactant, has maximum elasticity and deformability, and further has maximized transdermal capacity. Because the deformed vesicles are highly hydrophilic vesicles, when the vesicle-containing formulation is applied to the skin, the vesicles will tend to penetrate the skin barrier and migrate to the deep layer rich in moisture, achieving good penetration.

Preferably, the preparation raw materials of the deformation vesicle comprise, by weight, 4-10 parts of polyglycerol ester surfactant, 2-5 parts of sorbitan ester surfactant, 1-3 parts of cetyl phosphate, 0.1-2 parts of honokiol and 1-3 parts of magnolol.

The mass part of the polyglycerol ester surfactant can be 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts or 10 parts and the like.

The sorbitan ester type surfactant can be 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts or 5 parts by mass and the like.

The cetyl phosphate may be 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, etc.

The mass part of honokiol can be 0.1 part, 0.5 part, 1 part, 1.2 parts, 1.4 parts, 1.6 parts, 1.8 parts or 2 parts, etc.

The magnolol may be 1 part, 1.2 parts, 1.4 parts, 1.6 parts, 1.8 parts, 2 parts, 2.2 parts, 2.4 parts, 2.6 parts, 2.8 parts, 3 parts, or the like by mass.

In a third aspect, the present invention provides a method for preparing a formulation having the efficacy of inhibiting melanin, improving dark-eyed circles and under-eye puffiness as described in the second aspect, the method comprising:

(1) Mixing the preparation raw materials of the deformed vesicle, and carrying out nanocrystallization treatment to obtain the deformed vesicle;

(2) The deformed vesicle obtained in the step (1) is mixed with the composition having the effects of inhibiting melanin and improving dark-eyed circles and the eye bags as described in the first aspect, and nanocrystallized to obtain the deformed vesicle coated with the composition having the effects of inhibiting melanin and improving dark-eyed circles and the eye bags as described in the first aspect.

The invention provides a preparation method of a preparation with the effects of inhibiting melanin and improving dark circles and eye bags, which is simple and convenient to operate and suitable for industrial scale-up.

Preferably, the nanocrystallization in step (1) comprises a high pressure high velocity microfluidic treatment.

Preferably, the nanocrystallization in step (2) comprises a high pressure high velocity microfluidic treatment.

Preferably, the preparation method further comprises the preparation method of the composition having the effects of inhibiting melanin, improving dark-eyed circles and under-eye puffiness as described in the first aspect in the step (2);

preferably, the preparation method of the composition having the effects of inhibiting melanin protein, improving black eye and eye pouch according to the first aspect in the step (2) comprises a preparation method of a fermentation extract of thyme;

Preferably, the preparation method of the thyme fermented extract comprises the steps of smashing thyme, uniformly mixing with a culture medium, inoculating thermophilic bacteria, fermenting and culturing for 30-60 hours, collecting fermentation liquor, carrying out ultrasonic wall breaking treatment, centrifuging, taking down a lysate, and sterilizing to obtain the thyme fermented extract;

the values in the range of 30-60h may be selected from 30h, 35h, 40h, 45h, 50h, 55h, 60h, etc., and other specific values in the ranges of the values may be selected, which will not be described in detail herein.

Preferably, the thermophilic bacteria include any one or a combination of at least two of thermophilic thermus bacteria, streptococcus thermophilus, bacillus stearothermophilus, streptococcus thermophilus, sporotrichum thermophilus or streptomyces thermophilus, and the combination of at least two of the thermophilic thermus bacteria and streptococcus thermophilus, the combination of streptococcus thermophilus and bacillus stearothermophilus, the combination of streptococcus thermophilus and sporotrichum thermophilus, and the like, and any other combination modes are selected and will not be repeated here.

It is well known to those skilled in the art that thermophilic thermus, streptococcus thermophilus, bacillus stearothermophilus, streptococcus lactis, sporotrichum thermophile or streptomyces thermophilus belongs to thermophilic bacteria, wherein thermophilic thermus is a heat-resistant strain, and cells contain high heat-resistant TaqDNA polymerase, so that the cells can resist the high temperature of more than 110 ℃, and the protein component has strong stability and self-repairing capability. The strain contains rich nutrients such as protein, nucleotide, vitamin and glutathione with antioxidant, detoxication and repair components, has obvious effects of diminishing inflammation, whitening, resisting aging, repairing skin, locking water and moisturizing, and further contains a molecular motor, wherein the molecular motor is an ATP synthase molecule, and the main function of the strain under physiological conditions is to synthesize ATP. When the skin lacks ATP, the molecular motor counter-rotates to generate ATP to power the cells. Under the condition that cells are damaged, the molecular motor can be used as a skin energy factor to promote the synthesis of ATP energy of the skin and bidirectionally regulate the skin function state.

In a fourth aspect, the present invention provides the use of a composition having the efficacy of inhibiting melanin, improving dark-eyedrops and eye bags as described in the first aspect or a formulation having the efficacy of inhibiting melanin, improving dark-eyedrops and eye bags as described in the second aspect in the preparation of a cosmetic.

Compared with the prior art, the invention has the following beneficial effects:

1. the invention creatively applies the combination of four components of the thymus fermented extract, the peony root extract, the lutein and the superoxide dismutase to the problems of dark circles and the eye bags, and mainly improves the problems of the dark circles and the eye bags from 3 aspects: the fermentation extract of thyme inhibits the expression of kinesin, reduces the cell transfer of melanin, and further relieves the symptoms of dark circles; the peony root extract has the effect of enhancing immunity, can play a role in repairing cell injury, further improve skin cell injury caused by external adverse factors, promote oxygen supply recovery of blood vessels around eyes and repair cell injury, and relieve the problems of dark circles and eye bags; lutein and superoxide dismutase have effects of scavenging free radicals and resisting oxidation, and can rapidly scavenge damaging free radicals generated by cell metabolism, and promote recovery of cell injury around eyes. The thyme fermented extract, the peony root extract, the lutein and the superoxide dismutase complement each other, and have synergistic effect, and have the effects of stronger antioxidation, dark circle fading, skin tightening and eye bag improvement.

2. The preparation with the effects of inhibiting melanin and improving dark circles and the eye bags contains the composition according to the first aspect, has the effects of resisting oxidization, weakening dark circles, tightening skin and improving the eye bags, and can be added with different auxiliary materials to replace different dosage forms according to actual requirements.

3. The invention also provides a preparation method of the preparation with the effects of inhibiting melanin and improving dark circles and eye bags, and the preparation method is simple and convenient to operate and suitable for industrial amplification.

Detailed Description

The technical scheme of the invention is further described by the following specific embodiments. It should be apparent to those skilled in the art that the examples are merely provided to aid in understanding the present invention and should not be construed as limiting the invention in any way.

The peony root extracts of the following examples, comparative examples and test examples are available from Guangzhong Biotech Co., ltd. Model 20; chlorella extract is a product purchased from Shaanxi Yi Chuan Biotechnology Co., ltd; the extract of thyme in example 10 was obtained from the company, utility biotechnology, inc. of Guangzhou, under the model number AJ-01-22; the Hamamelis mollis extract is available from AMERICAN DISTILLING INC company under the model number PHENOXYETHANOL; the lysates of the fermentation products of Saccharomyces cerevisiae were obtained as Repair Complex CLR PF from the company Chemisches Laboratorium Dr. Kurt Richter GmbH.

The specific techniques or conditions are not identified in the examples and are described in the literature in this field or are carried out in accordance with the product specifications. The reagents or apparatus used were conventional products commercially available through regular channels, with no manufacturer noted.

Preparation example 1

The preparation example provides a thyme fermented extract, and the preparation method comprises the following steps: washing whole plant of thyme, removing root, drying, pulverizing, and sieving with 120 mesh sieve. Adding into thermophilic Thermus culture medium (0.4% yeast powder, 0.8% polypeptone, 0.2% sodium chloride, 2% agar, and water for supplementing, and sterilizing) at mass ratio of 1%, and culturing thermophilic Thermus (ATCC 27634) according to 0.1% inoculation amount into 2L thermophilic Thermus culture medium at 75deg.C at 150rpm for 12 hr to obtain thermophilic Thermus activating solution;

inoculating thermophilic Thermus activating solution into thermophilic Thermus culture medium according to 1% inoculation amount, culturing at 75deg.C at 150rpm for 20 hr, stopping fermentation, collecting fermentation broth, performing ultrasonic wall breaking treatment (operating voltage 1800W) with ultrasonic breaker with model CB-26A of Kunshan Xiaomei, centrifuging at 3000rpm for 15min, collecting lower layer lysate, and ultraviolet sterilizing for 30min to obtain herba Thymi fermentation extract.

Example 1

The embodiment provides a composition with the effects of inhibiting melanin and improving black eyes and eye bags, which comprises the following components in parts by mass: 1 part of thyme fermented extract, 1 part of peony root extract, 0.03 part of lutein, 0.01 part of superoxide dismutase, 2 parts of 3-o-ethyl ascorbic acid, 1 part of adenosine, 1 part of carnosine, 0.01 part of tetrapeptide-1, 0.2 part of caffeine, 0.8 part of chlorella extract, 0.5 part of witch hazel extract, 1 part of a lysate of a fermentation product of two-part yeast, and 40 parts of water are added, and the above components are stirred and mixed uniformly to prepare the composition with the effects of inhibiting melanin protein and improving black eyes and eye bags.

Example 2

The embodiment provides a composition with the effects of inhibiting melanin and improving black eyes and eye bags, which comprises the following components in parts by mass: 6 parts of thyme fermented extract, 0.5 part of peony root extract, 0.2 part of lutein, 0.05 part of superoxide dismutase, 1 part of 3-o-ethyl ascorbic acid, 2 parts of adenosine, 0.5 part of carnosine, 0.001 part of tetrapeptide-1, 0.1 part of caffeine, 5 parts of chlorella extract, 0.8 part of witch hazel extract, 7 parts of a lysate of a fermentation product of the schizosaccharomyces, and 40 parts of water are added, and the above components are stirred and mixed uniformly to prepare the composition with the effects of inhibiting melanin and improving black eyes and bags.

Example 3

The embodiment provides a composition with the effects of inhibiting melanin and improving black eyes and eye bags, which comprises the following components in parts by mass: 3 parts of thyme fermented extract, 2 parts of peony root extract, 0.01 part of lutein, 0.04 part of superoxide dismutase, 1.5 parts of 3-o-ethyl ascorbic acid, 0.6 part of adenosine, 2 parts of carnosine, 0.05 part of tetrapeptide-1, 1 part of caffeine, 0.5 part of chlorella extract, 1 part of witch hazel extract, 10 parts of lysate of fermentation product of two-split yeasts, and 40 parts of water supplement, and stirring and uniformly mixing the above components to prepare the composition with the effects of inhibiting melanin and improving black eyes and eye bags.

Example 4

This example provides a composition having effects of inhibiting melanin protein and improving black eye and eye pouch, which is different from example 1 only in that the preparation raw materials do not contain chlorella extract, witch hazel extract and lysates of fermentation products of bifenthrin, and the mass parts of chlorella extract, witch hazel extract and lysates of fermentation products of bifenthrin are proportionally distributed to thyme fermentation extract, peony root extract, lutein, superoxide dismutase, and the other are the same as example 1.

Example 5

This example provides a composition having effects of inhibiting melanin protein and improving black eye and eye pouch, which is different from example 1 only in that the preparation raw material does not contain chlorella extract, and the mass fraction of chlorella extract is proportionally distributed to Hamamelis mollis extract, yeast extract lysate of two-split yeast, and the other is the same as example 1.

Example 6

This example provides a composition having effects of inhibiting melanin protein and improving black eye and eye pouch, which is different from example 1 only in that the preparation raw material does not contain witch hazel extract, and the mass fraction of the witch hazel extract is proportionally distributed to chlorella extract, a fermentation product lysate of schizosaccharomyces, and the other is the same as example 1.

Example 7

This example provides a composition having effects of inhibiting melanin protein and improving black eye and eye pouch, which is different from example 1 only in that the preparation raw material does not contain a lysate of a fermentation product of Saccharomyces cerevisiae, and the mass fraction of the lysate of the fermentation product of Saccharomyces cerevisiae is proportionally distributed to Chlorella extract and Hamamelis mollis extract, and the other is the same as example 1.

Example 8

This example provides a composition having effects of inhibiting melanin protein and improving black eye and eye pouch, which is different from example 1 only in that the preparation raw material does not contain witch hazel extract, yeast for bifenthrin fermentation product lysate, and the mass fraction of the witch hazel extract, yeast for bifenthrin fermentation product lysate is all distributed to chlorella extract, otherwise the same as example 1.

Example 9

This example provides a composition having effects of inhibiting melanin protein and improving black eye and eye pouch, which is different from example 1 only in that the preparation raw materials do not contain chlorella extract, witch hazel extract, and the mass fraction of chlorella extract, witch hazel extract is all distributed to the lysate of the fermentation product of the saccharomyces cerevisiae, and the other is the same as example 1.

Example 10

This example provides a composition having melanin inhibiting, black eye improving and eye bag improving effects, which differs from example 1 only in that the fermented extract of thyme prepared in preparation example 1 of the preparation materials is replaced with the extract of thyme purchased from the company Uyolkovata biosciences, inc. model AJ-01-22, and all other things are the same as in example 1.

Comparative example 1

This comparative example provides a composition having effects of inhibiting melanin protein, improving dark circles and under-eye puffiness, which is different from example 1 only in that the preparation raw material does not contain an thyme fermented extract, and the mass fraction of the thyme fermented extract is proportionally distributed to peony root extract, lutein and superoxide dismutase, all the other being the same as in example 1.

Comparative example 2

This comparative example provides a composition having effects of inhibiting melanin protein, improving black eyes and eye bags, which is different from example 1 only in that the preparation raw material does not contain peony root extract, and the mass fraction of the peony root extract is proportionally distributed to thyme fermented extract, lutein and superoxide dismutase, and the other is the same as example 1.

Comparative example 3

This comparative example provides a composition having effects of inhibiting melanin protein, improving black eyes and eye bags, which is different from example 1 only in that lutein is not contained in the preparation raw material, and the mass fraction of lutein is proportionally distributed to thyme fermented extract, peony root extract and superoxide dismutase, and otherwise is the same as example 1.

Comparative example 4

This comparative example provides a composition having effects of inhibiting melanin protein, improving black eyes and eye bags, which is different from example 1 only in that the preparation raw material does not contain superoxide dismutase, and the mass parts of the superoxide dismutase are proportionally distributed to the thyme fermented extract, the peony root extract and lutein, and the other is the same as example 1.

Comparative example 5

This comparative example provides a composition having effects of inhibiting melanin protein and improving dark circles and under-eye puffiness, which is different from example 1 only in that the preparation raw materials of the thyme fermented extract, the peony root extract, lutein, and superoxide dismutase are replaced with the thyme fermented extract, and the peony root extract, lutein, and superoxide dismutase are all distributed to the thyme fermented extract in parts by mass, and the other are the same as example 1.

Comparative example 6

This comparative example provides a composition having effects of inhibiting melanin protein and improving dark circles and under-eye puffiness, which is different from example 1 only in that the preparation raw materials of the thyme fermented extract, the peony root extract, lutein, and superoxide dismutase are replaced with the peony root extract, and the parts by mass of the thyme fermented extract, lutein, and superoxide dismutase are all distributed to the peony root extract, and the other are the same as example 1.

Comparative example 7

This comparative example provides a composition having effects of inhibiting melanin protein and improving dark circles and under-eye puffiness, which is different from example 1 only in that the preparation raw materials of the thyme fermented extract, the peony root extract, lutein, and superoxide dismutase are replaced with lutein, and the parts by mass of the thyme fermented extract, the peony root extract, and the superoxide dismutase are all distributed to lutein, and the other are the same as example 1.

Comparative example 8

This comparative example provides a composition having effects of inhibiting melanin protein and improving dark circles and under-eye puffiness, which is different from example 1 only in that the thyme fermented extract, peony root extract, lutein, and superoxide dismutase in the preparation raw materials are replaced with superoxide dismutase, and the whole parts by mass of the thyme fermented extract, peony root extract, and lutein are distributed to the superoxide dismutase, and the other parts are the same as in example 1.

Comparative example 9

This comparative example provides a composition having effects of inhibiting melanin protein and improving black eye and eye pouch, which is different from example 1 only in that the preparation raw materials do not contain the thyme fermented extract, peony root extract, lutein, superoxide dismutase, and the parts by mass of the thyme fermented extract, peony root extract, lutein, superoxide dismutase are proportionally distributed to chlorella extract, witch hazel extract and yeast extract lysate, and the other are the same as example 1.

Application example 1

The application example provides a preparation with the effects of inhibiting melanin and improving black eyes and eye bags, and the preparation method comprises the following steps:

(1) 6 parts of polyglycerol ester surfactant (3 parts of polyglycerol-6 ester of wild almond oil and 3 parts of polyglycerol-10 oleate), 3.8 parts of sorbitan ester surfactant (3.8 parts of sorbitan palmitate), 2 parts of cetyl phosphate, 0.2 part of honokiol and 1 part of magnolol are uniformly mixed, and the mixture is put into a high-pressure nano microfluidizer, and parameters are set: and (3) adding 40 parts of the composition with the effects of inhibiting melanin and improving dark-eyed circles and the eye bags prepared in the example 1 after continuously shearing for 5min at the shearing speed of 500m/s, and continuously homogenizing for 3min to obtain the deformation vesicles wrapping the composition with the effects of inhibiting melanin and improving dark-eyed circles and the eye bags.

(2) And (3) uniformly mixing 20 parts of deformation vesicles, 70 parts of butter oil and 10 parts of ethylhexyl palmitate, which are coated with the composition with the effects of inhibiting melanin and improving dark circles and under-eye bags, which are obtained in the step (1), so as to obtain the preparation with the effects of inhibiting melanin and improving dark circles and under-eye bags.

Application examples 2 to 8

Application examples 2 to 8 provided 7 formulations having melanin inhibiting, black eye improving and pouch efficacy, the preparation method thereof was different from application example 1 only in that the composition having melanin inhibiting, black eye improving and pouch efficacy prepared in example 1 was replaced with the composition having melanin inhibiting, black eye improving and pouch efficacy prepared in examples 4 to 10, and the rest was the same as application example 1.

Comparative examples 1 to 9 were used

Application comparative examples 1 to 9 provided 9 formulations having melanin inhibiting, black eye improving and pouch efficacy, the preparation method thereof was different from application example 1 only in that the composition having melanin inhibiting, black eye improving and pouch efficacy prepared in example 1 was replaced with the composition having melanin inhibiting, black eye improving and pouch efficacy prepared in comparative examples 1 to 9, and the rest was the same as application example 1.

Comparative example 10 was used

The present application comparative example provides a preparation having the effects of inhibiting melanin protein and improving dark circles and bags, and the preparation method is different from the application example 1 only in that the preparation process does not contain deformed vesicles, and the specific preparation method is replaced by: 15.1 parts of the composition with melanin inhibiting, black eye improving and pouch improving effects prepared in example 1, 70 parts of avocado oil, and 10 parts of ethylhexyl palmitate were uniformly mixed, and the rest was the same as in application example 1.

Test example 1

The antioxidant properties of the compositions having the effects of inhibiting melanin, improving dark-eyedrops and pouch prepared in examples 1 to 10 and comparative examples 1 to 9 were evaluated by testing the clearance efficiency of DPPH. The specific evaluation method comprises the following steps: the composition was ground to homogeneity using a mortar, 0.5g of the composition was added to 4mL of DPPH (100. Mu.M in 99wt% ethanol) solution, mixed homogeneously, and incubated in the dark for 60min. The absorbance value of the mixed solution at 517nm was then measured using an ultraviolet-visible spectrophotometer (using a solution containing DPPH alone as a control). The following equation is used for calculation: DPPH clearance = (control absorbance-each example or comparative absorbance)/control absorbance x 100%.

TABLE 1

	DPPH clearance (%)
		Example 1	75
Example 2	68
		Example 3	71
Example 4	54
		Example 5	61
Example 6	65
		Examples7	58
Example 8	55
		Example 9	48
Example 10	47
		Comparative example 1	33
Comparative example 2	46
		Comparative example 3	37
Comparative example 4	43
		Comparative example 5	34
Comparative example 6	31
		Comparative example 7	37
Comparative example 8	26
		Comparative example 9	19

As can be seen from the data in Table 1, the compositions of example 4 and comparative example 9, compared with example 1, do not contain chlorella extract, witch hazel extract and lysate of fermentation products of two yeasts or do not contain four components of thyme fermentation extract, peony root extract, lutein and superoxide dismutase, and the DPPH clearance rate is lower than that of example 1, which indicates that the four components of thyme fermentation extract, peony root extract, lutein and superoxide dismutase can be combined with the three components of chlorella extract, witch hazel extract and lysate of fermentation products of two yeasts to synergistically increase, and the composition containing the two components has stronger antioxidation effect;

compared with the preparation raw materials in the example 1, the preparation raw materials in the comparative examples 1-8 respectively contain no one component or only one component of the four components of the thyme fermentation extract, the peony root extract, the lutein and the superoxide dismutase, and the DPPH clearance rate is lower than that in the example 1, so that the four components are matched with each other, and the preparation raw materials have synergistic effect and have excellent effects of scavenging free radicals and resisting oxidation.

Test example 2

The ATP content of the compositions having the effects of inhibiting melanin, improving dark-eyed circles and under-eye puffiness prepared in examples 1 and 10 was measured as follows: taking 1g of the composition with the effects of inhibiting melanin protein and improving black eyes and bags prepared in each example, and adding 10mL of physiological saline for dissolution; (2) ATP was detected using an ATP detection kit (Biyun, model S0026), and fluorescence values at 5min and 10min were measured on a fluorescence chemiluminescence analyzer, and the ATP content in example 1 and example 10 was compared according to the magnitude of the fluorescence values.

TABLE 2

	Fluorescence value for 5min	Fluorescence value for 10min
			Example 1	484833	763766
Example 10	113242	107861

As is clear from Table 2, the higher the fluorescence value, the higher the ATP content, the more molecular motor and ATP are used as the extract of the European thyme used in example 10, the lower the ATP content at 5min and 10min is compared with example 1, and the ATP content at 10min is higher than the ATP content at 5min in example 1, indicating that the extract of the European thyme fermented by the fermentation extraction of thermophilic bacteria has more molecular motor and ATP, and further, more molecular motor can be used as skin energy factor, promote the synthesis of skin ATP energy, and bidirectionally regulate skin function state.

Test example 3

The melanin removal performance of the preparation with the effects of inhibiting melanin and improving dark-eyed circles and bags prepared in application examples and application comparative examples is evaluated, and the specific method is as follows: (1) 340 zebra fish were randomly assigned to 17 groups, each group corresponding to one application example or application comparative example, each group having 20 zebra fish. (2) 1g of the preparation is dissolved in 10mL of physiological saline, the zebra fish is put into the physiological saline containing the preparation, after the zebra fish is incubated for 15min, the head of the zebra fish is photographed, and the melanin signal intensity of the head of the zebra fish is analyzed by using advanced image processing software Lightrom. (3) Zebra fish was added to 5 volumes of PBS solution, homogenized using a homogenizer, and subsequently tyrosinase activity was detected. (4) tyrosine activity detection method: 0.5g of homogenate is taken, the temperature is 4 ℃, the centrifugation is carried out at 12000rpm for 15min, and the supernatant is taken for standby. The protein content of the supernatant was determined by coomassie brilliant blue method using bovine serum albumin as standard protein. The supernatant containing 100. Mu.g of protein per well was transferred to a 96-well plate, and 100. Mu.L of 1mM dopamine solution and 100. Mu.L of PBS solution were added to each well, and reacted in a water bath at 37℃for 1 hour in the dark, absorbance A490 was measured at 490nm, the melanin signal intensity and tyrosinase activity of application example 1 were set to 100%, and the melanin signal intensity and tyrosinase activity of the other groups were expressed as relative percentages of the melanin signal intensity and tyrosinase of application example 1. The melanin removal effect of the test sample is reflected by the skin melanin signal intensity and the relative percentage of tyrosinase activity.

TABLE 3 Table 3

As can be seen from the data in table 3, the compositions having effects of inhibiting melanin, improving black eye and eye pouch used in application example 2 and application comparative example 9 are derived from example 4 and comparative example 9, respectively, and example 4, comparative example 9, compared with example 1, do not contain three components of chlorella extract, witch hazel extract and yeast extract lysate of bifidus or do not contain four components of thyme extract, peony root extract, lutein, superoxide dismutase, the relative percentage of melanin signal intensity is higher than that of application example 1, the relative percentage of tyrosinase activity is higher than 100%, the effect of inhibiting melanin generation is poor, and it is shown that the four components of thyme extract, peony root extract, lutein, superoxide dismutase can be combined with three components of chlorella extract, witch hazel extract and yeast extract lysate of bifidus, and the preparation containing two components has a stronger effect of removing body melanin.

The compositions with effects of inhibiting melanin and improving black eyes and eye bags, which are applied in the preparation raw materials of application examples 3-7, are respectively derived from examples 5-9, and the preparation raw materials of examples 5-9 respectively lack one or only one of three components of chlorella extract, witch hazel extract and fermentation product lysate of the two yeasts, compared with the preparation raw materials of example 1, the prepared preparation products have higher relative percentage of melanin signal intensity than the preparation raw materials of application example 1, the relative percentage of tyrosinase activity is higher than 100%, the effect of reducing melanin content is poor, which indicates that the three components of chlorella extract, witch hazel extract and fermentation product lysate of the two yeasts are synergistic in terms of reducing melanin production, and have better improving effect.

The composition with the effects of inhibiting melanin and improving black eyes and bags used in comparative examples 1-8 is derived from comparative examples 1-8, and the preparation raw materials used in comparative examples 1-8 respectively lack one component or only one component of four components of thyme fermented extract, peony root extract, lutein and superoxide dismutase, compared with example 1, and the relative percentage of melanin signal intensity is higher than 100%, the relative percentage of tyrosinase activity is higher than 100%, the effect of eliminating melanin is poor, and the effect of inhibiting melanin generation is poor, so that the combination of the four components of thyme fermented extract, peony root extract, lutein and superoxide dismutase is synergistic, and the effect of reducing melanin in zebra fish bodies is stronger.

Compared with the preparation in the application example 1, the preparation with the effects of inhibiting melanin and improving black eyes and bags prepared in the application example 10 does not contain deformation vesicles, the relative percentage of melanin signal intensity is higher than that in the application example 1, the relative percentage of tyrosinase activity is higher than 100%, the effect of eliminating melanin and inhibiting melanin generation is poor, and the deformation vesicles can promote the penetration of the composition into skin, promote the composition to inhibit melanin generation and accelerate melanin degradation.

Test example 4

The preparation with the effects of inhibiting melanin protein and improving dark-eyed eyes and eye bags prepared in application examples and application comparative examples is subjected to evaluation of the treatment effect of dark-eyed eyes and eye bags, and the specific evaluation method is as follows: (1) 180 volunteers are selected, healthy women aged 20-45 years are selected and divided into 18 groups, each group is 10 persons, the same formula product is used in the group, and one group corresponds to one application example or application comparison example; (2) Skin melanin values were detected by skin color analyzer CL400 and the pouch volume was analyzed by Primos facial image acquisition. (3) The volunteers use the product twice a day, 2mL of the product is smeared on the periocular skin, the product is rubbed for 30 seconds after being smeared, the product is smeared uniformly in a circular area with the diameter of 4mL, the periocular skin melanin value and the eye pocket volume of each volunteer on the 0 th day and the 10 th day are recorded respectively for 10 days, and the average value of the ratio of the use cases of each application example or the application comparison example is calculated, wherein the ratio is the periocular skin melanin value corresponding to the 10 th day, the eye pocket volume divided by the periocular skin melanin value corresponding to the 0 th day, and the eye pocket volume.

TABLE 4 Table 4

	Skin melanin value ratio	Pouch volume ratio
			Application example 1	0.21	0.36
Application example 2	0.40	0.54
			Application example 3	0.29	0.32
Application example 4	0.37	0.36
			Application example 5	0.30	0.38
Application example 6	0.35	0.41
			Application example 7	0.46	0.59
Application example 8	0.49	0.53
			Comparative example 1 was used	0.72	0.63
Comparative example 2 was used	0.63	0.62
			Comparative example 3 was used	0.52	0.59
Comparative example 4 was used	0.59	0.63
			Comparative example 5 was used	0.75	0.71
Comparative example 6 was used	0.83	0.74
			Comparative example 7 was used	0.78	0.72
Comparative example 8 was used	0.87	0.91
			Comparative example 9 was used	0.94	0.89
Comparative example 10 was used	0.82	0.81

As can be seen from the data of table 4, the compositions having effects of inhibiting melanin, improving black eye and eye pouch used in application example 2 and application comparative example 9 are derived from example 4 and comparative example 9, respectively, and example 4, comparative example 9, compared with example 1, do not contain three components of chlorella extract, witch hazel extract and yeast extract lysate of two parts or do not contain four components of thyme extract, peony root extract, lutein, superoxide dismutase, the skin melanin value ratio and eye pouch volume ratio of which are higher than those of application example 1, and the effect of improving black eye and eye pouch problems is poor, indicating that four components of thyme extract, peony root extract, lutein, superoxide dismutase can be combined with three components of chlorella extract, witch hazel extract and yeast extract lysate of two parts to synergistically enhance, and the preparation containing two components has stronger effect of fading black eye and improving eye pouch.

The compositions with effects of inhibiting melanin, improving black eyes and eye bags, which are applied in the preparation raw materials of application examples 3-7, are respectively derived from examples 5-9, and compared with example 1, the preparation raw materials of examples 5-9 respectively lack one or only one of three components of chlorella extract, witch hazel extract and yeast fermentation product lysate, and the prepared preparation product has high skin melanin value ratio and eye bag volume ratio compared with application example 1, which indicates that the three components of chlorella extract, witch hazel extract and yeast fermentation product lysate are synergistic in the aspects of reducing black eyes, alleviating eye bag problems and the like, and has better improvement effect.

The commercial thyme extract is used in the preparation raw material of application example 8, and the skin melanin value ratio and the eye bag volume ratio of the thyme extract are higher than those of application example 1, so that the thyme extract has better effects of removing melanin and relieving eye bags after being fermented by thermophilic bacteria.

The composition with the effects of inhibiting melanin and improving dark circles and under-eye bags used in comparative examples 1-8 is derived from comparative examples 1-8, and the preparation raw materials used in comparative examples 1-8 are respectively lack of one component or only one component of four components of thyme fermentation extract, peony root extract, lutein and superoxide dismutase compared with example 1, and the ratio of skin melanin value and the ratio of under-eye bags are higher than those of application example 1, so that after 10 days of use, the effects of improving dark circles and under-eye bags by using comparative examples 1-8 are worse than those of application example 1, and the effects of repairing dark circles and under-eye bags by using comparative examples 1-8 are shown to be stronger by synergistic effect by combining the four components of thyme fermentation extract, peony root extract, lutein and superoxide dismutase.

Compared with application example 1, the preparation with the effects of inhibiting melanin and improving dark-eyed circles and bags prepared in application comparative example 10 does not contain deformation vesicles, and the ratio of the melanin value of the skin and the volume ratio of the bags are higher than those of application example 1, so that the deformation vesicles can promote the composition to permeate into the skin, and the stronger repairing effect of the dark-eyed circles and bags is achieved.

Test example 5

Safety evaluation is carried out on the preparation with the effects of inhibiting melanin protein and improving black eyes and eye bags, which is prepared in application examples and application comparative examples, wherein the specific evaluation method is a cosmetic-eye irritation test chick embryo chorioallantoic membrane test (T/SHRH 011-2018), and the specific evaluation process is as follows: (1) Selecting fertilized chick embryos of Bai Laihang chickens and the like, hatching the fertilized chick embryos to an 8 th daily egg candler for checking, discarding unfertilized and inactive chick embryos, selecting chick embryos with good vascular development, and marking the positions of air chambers on the surfaces of eggshells. The test was started on day 9. The air chamber eggshell part is carefully stripped by dental bending, a few drops of physiological saline are dripped on the surface of the eggshell membrane to fully wet, and after pouring out, the eggshell membrane is carefully removed by a camera, so that the exposed allantoic membrane (Chorioallantoic membrane, CAM) is completely and completely free from any damage. 0.3mL/0.3g of the test sample was directly applied to the CAM. Coating is carried out as much as possible, and the coverage area is ensured to reach at least 50%. After 3min of action, the CAM surface was gently rinsed with physiological saline, the rinsing was completed within 30s, the liquid was decanted, and immediately the extent of each toxic effect change was observed under a stereoscopic microscope, and a score (ES) was given according to the evaluation criteria described in Table 3. Judging the basis: and (3) judging according to the grading (ES). ES is not more than 4 and not more than 12, and is slightly stimulated, 12 and 16, and is moderately stimulated, and ES is not less than 16, and is severely stimulated.

The specific evaluation is shown in the following table 5:

TABLE 5

Each group of samples corresponds to 6 chick embryos, and test results show that under the condition that the concentration of a test object is 100%, the ES of all test samples (skin repair compositions provided by application examples 1-8 and application comparative examples 1-10) is less than or equal to 4, and the preparation with the effects of inhibiting melanin and improving dark circles and eye bags is safe and non-irritating, and has higher use value.

The applicant states that the process of the invention is illustrated by the above examples, but the invention is not limited to, i.e. it does not mean that the invention has to be carried out in dependence on the above examples. It should be apparent to those skilled in the art that any modification of the present invention, equivalent substitution of raw materials for the product of the present invention, addition of auxiliary components, selection of specific modes, etc., falls within the scope of the present invention and the scope of disclosure.

Claims

1. A composition having melanin inhibiting, black eye improving and pouch improving effects, characterized in that the composition comprises a thyme fermented extract, a peony root extract, lutein and superoxide dismutase;

the composition comprises, by mass, 1-10 parts of thyme fermentation extract, 0.5-3 parts of peony root extract, 0.01-0.5 part of lutein and 0.001-0.05 part of superoxide dismutase;

The composition is encapsulated in nano-deformable vesicles.

2. The composition of claim 1 having melanin inhibiting, black eye and eye pouch improving effects, wherein the composition further comprises a small molecule active.

3. The composition of claim 2, wherein the small molecule active comprises any one or a combination of at least two of 3-o-ethyl ascorbic acid, adenosine, carnosine, tetrapeptide-1, or caffeine.

4. The composition for inhibiting melanin, improving black eye and eye pouch according to claim 3, wherein the small molecule active substance comprises any one or a combination of at least two of 1 to 5 parts by mass of 3-o-ethyl ascorbic acid, 0.5 to 5 parts by mass of adenosine, 0.5 to 5 parts by mass of carnosine, 0.001 to 0.05 parts by mass of tetrapeptide-1, or 0.1 to 1 part by mass of caffeine.

5. The composition having the efficacy of inhibiting melanin, improving dark-eyed circles and under-eye puffiness of claim 1, further comprising any one or a combination of at least two of chlorella extract, witch hazel extract or lysates of fermentation products of saccharomyces cerevisiae.

6. The composition having the efficacy of inhibiting melanin protein, improving dark under eye circles and under-eye puffiness of claim 5, further comprising a combination of chlorella extract, witch hazel extract, and lysates of fermentation products of saccharomyces cerevisiae.

7. The composition for inhibiting melanin protein and improving black eye and eye pouch of claim 6, further comprising 0.5-5 parts of chlorella extract, 0.5-5 parts of witch hazel extract, and 0.5-10 parts of lysate of fermentation product of saccharomyces cerevisiae in parts by weight.

8. A formulation having effects of inhibiting melanin, improving dark-eye circles and under-eye bags, characterized in that the formulation is prepared from a raw material comprising deformed vesicles and the composition having effects of inhibiting melanin, improving dark-eye circles and under-eye bags as claimed in any one of claims 1 to 7 wrapped in deformed vesicles.

9. The formulation for inhibiting melanin and improving the efficacy of dark-eyedrops and eye bags of claim 8, wherein the deformed vesicles are prepared from the raw materials comprising polyglycerol ester surfactant, sorbitan ester surfactant, cetyl phosphate, honokiol and magnolol.

10. The formulation for inhibiting melanin and improving dark-eyedrops and eye bags of claim 9, wherein the deformed vesicles are prepared from 4-10 parts by mass of polyglycerol ester surfactant, 2-5 parts by mass of sorbitan ester surfactant, 1-3 parts by mass of cetyl phosphate, 0.1-2 parts by mass of honokiol and 1-3 parts by mass of magnolol.

11. A method for preparing a formulation having the efficacy of inhibiting melanin, improving dark-eyed circles and under-eye puffiness according to any one of claims 8 to 10, characterized in that the method comprises:

(2) Mixing the deformed vesicle obtained in the step (1) with the composition for inhibiting melanin and improving dark-eyed circles and the eye bags according to any one of claims 1 to 7, and carrying out nanocrystallization to obtain the deformed vesicle coated with the composition for inhibiting melanin and improving dark-eyed circles and the eye bags according to any one of claims 1 to 7.

12. The method for preparing a formulation having melanin inhibiting, black eye improving and pouch efficacy of claim 11, wherein the nanocrystallization treatment in step (1) comprises a high pressure high speed micro-jet treatment.

13. The method for preparing a formulation having melanin inhibiting, black eye improving and pouch efficacy of claim 11, wherein the nanocrystallization treatment in step (2) comprises a high pressure high speed micro-jet treatment.

14. A method for preparing a formulation having the efficacy of inhibiting melanin, improving dark-eyedrops and eye bags according to claim 11, which further comprises the method for preparing a composition having the efficacy of inhibiting melanin, improving dark-eyedrops and eye bags according to any one of claims 1 to 7 in step (2).

15. The method for preparing a formulation having the efficacy of inhibiting melanin, improving dark-eyedrops and eye bags according to claim 14, wherein the method for preparing a composition having the efficacy of inhibiting melanin, improving dark-eyedrops and eye bags according to any one of claims 1 to 7 in the step (2) comprises a method for preparing a fermentation extract of thyme.

16. The method for preparing the preparation with the effects of inhibiting melanin and improving dark circles and under-eye puffiness of claim 15, wherein the method for preparing the fermentation extract of the thyme comprises the steps of smashing the thyme, uniformly mixing the smashed thyme with a culture medium, inoculating thermophilic bacteria, fermenting and culturing for 30-60 hours, collecting fermentation liquor, carrying out ultrasonic wall breaking treatment, centrifuging, taking out the lower layer lysate, and sterilizing to obtain the fermentation extract of the thyme.

17. The method for preparing a formulation having melanin inhibiting, black eye improving and pouch efficacy of claim 16, wherein the thermophilic bacteria comprises any one or a combination of at least two of thermophilic thermus, streptococcus thermophilus, bacillus stearothermophilus, streptococcus lactis, sporotrichum thermophile or streptomyces thermophilus.

18. Use of the composition having the efficacy of inhibiting melanin, improving dark-eyed circles and under-the-eye puffiness as claimed in any one of claims 1 to 7 or the preparation having the efficacy of inhibiting melanin, improving dark-eyed circles and under-eye puffiness as claimed in any one of claims 8 to 10 for the preparation of cosmetics.