JP2006069969A - Biofunction adjusting agent having active oxygen-removing function and cosmetic composition and beverage and food composition including the same - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a biofunction adjusting agent having active oxygen-removing function and provide a cosmetic composition and beverage and food composition including the same. <P>SOLUTION: Active oxygen (sometimes called a free radical) is greatly paid attention as a large cause of diseases and senescence. The present invention provides a biofunction adjusting agent having active oxygen-removing function that includes a fermentation solution prepared by mixing soybean extract, rush extract, and krill extract and fermenting the mixed solution with Bacillus natto. This invention further provides cosmetics, beverages or foods having active oxygen-removing function by using the fermentation solution prepared by mixing soybean extract, rush extract, and krill extract and fermenting the mixture with Bacillus natto. The fermentation solution has no side effect and can be used as daily health-maintaining agents, cosmetics, beverages. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a biological function regulator having a function of removing active oxygen, and a cosmetic and food / beverage composition containing the same.

Active oxygen (sometimes referred to as free radicals) has attracted much attention in recent years as a cause of illness and aging. How to prevent the harm of active oxygen is a big issue for civilians to live healthy. Moreover, it has been found that the generation of active oxygen can be reduced in the lifestyle, and scavengers, which are substances that exclude active oxygen, are taken from foods and the like.

Although humans can't live without oxygen, oxygen can hurt people, but as a by-product of oxygen, active oxygen is created and cells are damaged. When active oxygen is produced in the body, tissue and cellular components and enzymes essential for metabolism are damaged. Moreover, these active oxygens are always produced when oxygen reacts with enzymes in the body. Naturally, humans will be killed by active oxygen if they remain as they are, but active oxygen is characterized by a very short life span, and many substances (scavengers) that eliminate the poison of active oxygen are created in the process of human development as well as immunity. Protect your body.
Many adult diseases are affected by active oxygen.

Arteriosclerosis is caused by the fact that active oxygen oxidizes LDL cholesterol, known as bad cholesterol, by lipid peroxidation at the blood vessel wall. Active oxygen is also involved in ischemic diseases such as cerebral infarction and myocardial infarction. Cerebral vascular dementia is also related to arteriosclerosis, and active oxygen is a distant cause, but changes in cranial nerve fibers and senile plaques in Alzheimer's disease are said to cause lipid peroxidation due to active oxygen. In the first place, the brain consumes about one-fifth of the total oxygen, and a large amount of active oxygen is generated proportionally. It is thought that blurring will begin when the functions of the scavengers decline after they get older and the ability to generate scavengers falls.

A representative scavenger is SOD (superoxide dismutase), which is an enzyme that scavenges superoxide (peroxide), does not make active oxygen, removes it, repairs wounds, and protects lives. Yes. Foods having this SOD action include antioxidants such as vitamin E, vitamin C, vitamin B2, beta carotene, and vitamin A. There is also the idea that the life span of a living thing is that the scavenger's ability to produce declines, making it impossible to erase active oxygen. It is attracting attention as a cause of aging. Therefore, foods that protect the body from active oxygen prevent not only adult diseases but also aging.

Foods that protect the body from active oxygen include carrots, tomatoes, pumpkins, peppers, parsley, spinach, spring chrysanthemum, komatsuna, turnips, radish leaves, sesame seeds, germs, pearl barley, mushrooms, lemons, strawberries, mandarin oranges, oysters, ginkgo Leaves, dokudami, kumazasa, shijimi, root kombu, hijiki, beef, liver, butter, milk, egg, natto, fish meat, vegetable oil, green tea and the like are known.
Diseases related to reactive oxygen are skin cancer, other cancers, arteriosclerosis, cerebral infarction, myocardial infarction, diabetes, ventilation, diabetic retinopathy, cataract, gastric / duodenal ulcer, dementia, Parkinson's disease, aging, rheumatism Osteoarthritis, emphysema, etc. are known.

JP-A-2003-81744 (Patent Document 1) contains an extract from the above-ground part of licorice as an active ingredient. An antioxidant is disclosed in which the extract has an active oxygen scavenging action and / or a radical scavenging action.

In JP 2003-12538 A (Patent Document 2), a solid medium containing mycelium obtained by inoculating shiitake fungus on a solid medium based on Bacus and then growing the mycelium is unbundled. Then, water and one or more enzymes selected from cellulase, glucosidase or protease are added to the unbound solid medium while keeping the solid medium at 30 to 50 ° C., and the solid medium is added to the enzyme. Extracted shiitake mycelium extract by crushing and crushing in the presence of water, then inactivating the enzyme by heating to a temperature up to 95 ° C. and sterilizing the anti-oxidant effect comprising shiitake mycelium extract Agent. 2. The treatment and / or prevention of a disease involving oxidative cytotoxicity caused by reactive oxygen species or lipid peroxide, which comprises the shiitake mycelium extract and a pharmaceutically acceptable carrier as an optional component. Antioxidant agent. The disease is selected from the group consisting of liver disorders caused by drugs and harmful substances, cardiovascular diseases, gastrointestinal diseases such as gastric ulcers and gastric mucosal disorders, respiratory diseases, diabetic complications, skin diseases, cancer, and aging An antioxidant agent according to claim 2 is disclosed.

Japanese Patent Laid-Open No. 2002-275018 (Patent Document 3) discloses Udo (Aralia).
an external preparation for skin, comprising an extract obtained from (cordata) as an active oxygen removing component.
An external preparation for skin, comprising a hot water extract of udo (Aralia cordadata) as an active oxygen removing component. The skin external preparation characterized by containing the following component (A) and (B), (A) Udo extract (B) active oxygen removal agent. Active oxygen scavengers include carotenoids such as superoxide dismutase, mannitol, beta carotene, astaxanthin, lutein and animal and plant extracts containing these, hydroquinone and derivatives thereof, bilirubin, cholesterol, tryptophan, histidine, quercetin, quercitrin, catechin And derivatives thereof, rutin and derivatives thereof, taurine, thiotaurine, soybean or egg-derived phospholipids, eggshell membrane extract, placenta extract, gallic acid and its derivatives, stevia, grabridine, glabrene, liquiritin, isoliquiritin and the like Licorice extract, yeast extract, oxon extract, seaweed extract, tormentilla extract, rahan fruit extract, ginkgo biloba extract, ganoderma biloba extract, grape seed extract, keiketto extract, Tea extract such as tea, black tea, oolong tea, peony extract, gentian extract, hawthorn extract, rosemary extract, mokka extract, yukinoshita extract, yashajitsu extract, gennoshoco extract, button pi extract, melissa extract , Parsley extract, dicoppi extract, vitamin A and derivatives thereof, vitamin B and derivatives thereof, vitamin C and derivatives thereof, vitamin D and derivatives thereof, vitamin E and derivatives thereof, glutathione and derivatives thereof An external preparation for skin which is selected from dibutylhydroxytoluene and butylhydroxyanisole is disclosed.

Japanese Patent Application Laid-Open No. 2002-179581 (Patent Document 4) includes a water scent (Ludwigia octovalvis),
Fish needle grass (Anisomeles indica), half-branched lotus (Scutellaria rivularis), half-border lotus (Lobelia chinensis), moth mother grass (Urena lobata), horse whip grass (Verbana officinalis), lion (Cuscuta australis) ), Phyllanthus urinaria, Physalis angulata, Cleome gynandra, Rorippa indica, Hydrocotyle formosana, Vernonia cinerae, Spilanthes acmella ), Canna indica, Ipomoea pes-caprae, Fossil tree (Clerodendrum calamitosum), Drosera burmanni, Gold line lotus (Anoectochilus formosanus), Nanksa flower (Wikstroemia indica), White leaves Thorn root (Elaeagnus oldhamii), Jade flower (Mussaenda parviflora), Sengoku (Mesona procumbens), Taiwan horse suzu (Aristolochia mollis), Shiso (Acorus gramineus), Golden katsura (Cudrania)
cochinchinensis var. gerontogea), odor moth (Clerodendrum philippinum), large blue (Clerodendrum cyrtophyllum), white dragon ship flower (Clerodendrum paniculataum var. albifloraum), wood hempen (Boehmeria densiflora), acetabular (Sapium sebiferum) dentatum, Vitis thunbergii, Bauhinia championi, Bredia oldhami, Staurogyne concinnula
), Seiraginella
doederleinii), Koedako (Lespedeza cuneata), Hyodosochoka (Ludwigia hyssopifolia), Senganfuji (Stephania japonica), Sphenomeris chusana, Iron wire flank (Adiantum flabellulatum), Sanka (Hyptis suaveolens), Chicken eye grass ( Kummerowia striata), Haizhou Changshan (Clerodendrum trichotomum), Twilight (Vitex negundo), Xanthium strumarium var. Japonica, Bidens pilosa var. Minor, Gnaphalium affine, Purple back grass (Emilia sonchifolia), bonito (Mimosa pudica), larva (Euphorbia hirta), car forage (Plantago formosana), landed bell (Cardiospermum halicacabum), white flower wisteria (Plumbago zeylanica), nailed ridge (Torenia concolor var. Formosona ), Daegu grass (Euphorbia formosana), Viola phillippica, Polygonum
multiflorum var.hypoleucum), milk lees (Ficus erecta var. , Gold sword grass (Rubialanceolata), Safflower coccinea, Ixeris chinensis, Ixeris laevigata var. Oldhami, Clerodendrum inerme, Hyptis Contains one or more plant extracts selected from rhomboides, Callicarpa formosana, Aeginetia indica, Pratia nummularia, and Acanthopanax trifoliatus An anti-aging agent for skin.

Among the above extracts, anti-aging effects are melanin production inhibitory effects, such as Fish Anemone (Anisomeles indica), Bamboo Herb (Urena lobata), Lion (Cuscuta australis), Horseshoe Gold (Dichondra micrantha), Affair (Physalisangulata ), Cleome gynandra, Rorippa indica, Hydrocotyle formosana, Spilanthes acmella, Canna indica, Anoectochilus
formosanus), Wikstroemia indica, Taiwan potato bell (Aristolochia mollis), sarcophagus (Acorus gramineus), golden katsura (Cudrania cochinchinensis var.gerontogea), Daelao (Clerodendrum)
cyrtophyllum), Bauhinia championi, Selaginella doederleinii, Koedoko (Lespedeza cuneata), Hyodososuka (Ludwigia hyssopifolia), Xanthium strumarium, Bidens pilosa var.or. Purple back grass (Emilia sonchifolia), car front grass (Plantago formosana), white flower wisteria (Plumbagozeylanica), nail ridge (Torenia concolor var.formosona), navy blue violet flower place (Viola phillippica), milk eel (Ficus erecta var.beecheyana) An anti-aging agent characterized by comprising one or more plant extracts selected from Kalanchoe spathulata, Rubia lanceolata, and Salvia coccinea . Among the extracts, anti-aging effect is elastase inhibitory effect, Phyllanthus urinaria, Nanksa flower (Wikstroemia indica), Golden katsura (Cudrania cochinchinensis var. Gerontogea), Boehmeria densiflora, Three Tetrastigma dentatum, Vitis thunbergii, Bauhinia championi, Bredia oldhami, Lespedeza cuneata, Mizusaka Hoso (Ludwigia)
hyssopifolia), iron wire moth (Adiantum flabellulatum), ginseng grass (Mimosa pudica), fallen bell (Cardiospermumhalicacabum), white flower wisteria (Plumbago zeylanica), nail terrain (Torenia concolorvar. , Taiwan Saito (Uncaria hirsuta), Rhus semialata var.
An anti-aging agent comprising one or more plant extracts selected from roxburghiana).

Among the above extracts, the anti-aging effect is the removal of active oxygen, Ludwigia octovalvis, Anisoles indica, Scutellaria rivularis, Lobelia chinensis, Horse whip grass (Verbana officinalis), Cuscuta australis, Phyllanthusurinaria, Physalis angulata, Cleome gynandra, Vernonia cinerae, Canna indica, Ipomoea pes -caprae), fossil trees (Clerodendrum calamitosum), Nishikiji (Drosera burmanni), Nanksa (Wikstroemia indica), white leaves (Elaeagnus oldhamii), golden leaves (Mussaenda parviflora), sengoku (Mesona procumbens), Sarcophagus (Acorus gramineus), golden katsura (Cudrania)
cochinchinensis var. gerontogea), odor (Clerodendrum philippinum), large blue (Clerodendrum cyrtophyllum), white dragon ship flower (Clerodendrum paniculataum var. dentatum, Vitis thunbergii, Bauhinia championi, Bredia oldhami, Staurogyne concinnula, Lespedeza cuneata, Mizuoka Hodaka (Ludwigia)
hyssopifolia), Chigane Fuji (Stephania japonica), Iron wire shark (Adiantum flabellulatum), Sanka (Hyptis suaveolens), Haizhou Changshan (Clerodendrumtrichotomum), Twilight (Vitex negundo), Gnaphalium affine, Amigo grass (Mimosa pudica) ), Baby grass (Euphorbia hirta), nail eel (Torenia concolor var.formosona), large shell grass (Euphorbia formosana), Taiwan 何 (Polygonum multiflorum var. Hypoleucum), Taiwan Uncaria hirsuta, Rhus semialata var.
roxburghiana), plant front formosana, cardiospermum
halicacabum, Golden sword grass (Rubia lanceolata), Ixeris chinensis, Ixeris laevigata var. oldhami, Clerodendrum inerme, Hyptis rhomboides, Callicarpa formosana), wild boar (Aeginetia indica), copper cone ball grass (Pratia nummularia), and one or more plant extracts selected from Acanthopanax trifoliatus An inhibitor is disclosed.

JP-A-2002-173441 (Patent Document 5) describes an antioxidant or preventive agent for a living body containing an extract selected from one or more mushrooms and truffles belonging to the genus Mushroom. An active oxygen scavenging enzyme activity promoter comprising an extract selected from one or more mushrooms and truffles belonging to the genus Mannenmus. The enzyme activity promoter according to claim 2, wherein the active oxygen scavenging enzyme is superoxide dismutase (SOD) or catalase. An enzyme activity promoter is disclosed in which mushrooms belonging to the genus Mannentake are black ganoderma, ganoderma (Akashiba), purple turf, and maggot.

Japanese Patent Laid-Open No. 2001-199868 (Patent Document 6) contains the following components (A) and (B), (A) human body fluids or human body fluid-like liquid compositions (B) antioxidants. A featured external preparation. The liquid composition similar to the body fluid or human body fluid is one or two selected from extracellular fluid-plasma, tissue fluid, lymph fluid, connective tissue fluid, intracellular fluid, cell passage fluid, etc., or similar compositions thereof The external preparation characterized by mix | blending the above. The antioxidant is ascorbic acid, tocopherol, lignan, tannin, phytin, saponin, glycyrrhizin, pyrilbin, albumin, anthocyanin, ubiquinol, β-diketone, trachrisone, chlorophyll, carotenoid, glutathione, β-carotene and their derivatives, and SOD or SOD-like actives having active oxygen scavenging effect (the SOD or SOD-like actives are rosemary, sage, clove, thyme, okonomiyaki, oregano, ginger, cinnamon, dio, genus shoko, eucalyptus, tea, hamamelis, strawberry , Kale, spinach, roux, nutmeg, sesame, rice germ, marjoram, mace, funnel, ginkgo, mitsodezo, brown algae, coffee beans, zebrafish, Brussels sprouts, Alfalfa sprouts 1) selected from amino acids, phytic acid, citric acid, uric acid, rutin, terpenoids, polyphenols, flavonoids, catechins, pycnogenols, alkaloids, melanoidins, coenzymes, hydroxycinnamic acids and their derivatives or An external preparation comprising two or more kinds.

The liquid composition similar to the body fluid is a first group selected from the group consisting of sodium, potassium, calcium, magnesium, chlorine, phosphate, sulfate, carbonate, iron, fluorine, silicon, zinc, strontium A second group of trace elements selected from the group consisting of, rubidium, lead, manganese, copper, aluminum, cadmium, tin, barium, mercury, serine, iodine, molybdenum, nickel, boron, chromium, arsenic, cobalt, vanadium The first group of the fourth group of non-electrolytes selected from the group consisting of glucose, urea, creatinine, and pyrilvin, which is an ultratrace element selected from the group, is an essential component. 1 type or 2 or more types of components of each group of 2nd group thru | or 4th group are selected suitably, and it adds to 1st group, or these 2nd group External preparation, characterized in that without the addition of components optimal fourth group. In general, seawater that is considered to be close in composition to body fluids, that is, shallow seawater, deep seawater, sea salt, that is, shallow seawater salt, deep seawater salt, and the composition was adjusted by further adding insufficient components. An external preparation characterized by the above is disclosed.

Japanese Patent Application Laid-Open No. 2001-181173 (Patent Document 7) discloses a whitening agent having a tea extract as an active ingredient.
A whitening external preparation characterized by containing a tea extract. (A) White tea extract (B) Whitening agent for external use characterized by containing one or more medicinal agents selected from a whitening agent, an active oxygen remover, an antioxidant, an anti-inflammatory agent, and an ultraviolet light inhibitor .
The whitening agent of component (B) is glabrizine, glabrene, liquiritin, isoliquiritin and licorice extract containing these, hydroquinone and derivatives thereof, salts thereof, cysteine and derivatives thereof, salts thereof, ellagic acid and derivatives thereof and salts thereof , Vitamin C and derivatives thereof, and salts thereof, glutathione and derivatives thereof, and salts thereof, placenta extract, resorcin and derivatives thereof, and salts thereof, oyster extract, juniper extract, sempukuka extract, coconut extract, sun penz Extract, Sohakuhi extract, Toki extract, Ibukitorano extract, Clara extract, Hawthorn extract, Shirayuri extract, Hop extract, Neubara extract, Maikai extract, Gokahi extract, Mokka extract, Brown sugar Extract, wheat germ extract , Intin-koo extract, Coix extract, Aralia elata extract, bilberry extract, der whitening external preparation those selected from Altea extract and grape seed extract.

The active oxygen scavenger of component (B) is carotenoids such as SOD, mannitol, beta-carotene, astaxanthin, rutin and derivatives thereof, bilirubin, cholesterol, tryptophan, histidine, quercetin, quercitrin, catechin, catechin derivatives, gallic acid, Gallic acid derivatives, Ogon extract, Ginkgo biloba extract, Yukinosita extract, Melissa extract, Gennoshoko extract, Botpi extract, Parsley extract, Tormentilla extract, Rakan fruit extract, seaweed extract, Yashajitsu extract and Zikopi extract A whitening topical agent that is selected from products.

Antioxidant of component (B) is vitamin A and derivatives thereof and salts thereof, vitamin B and derivatives thereof, and salts thereof, vitamin D and derivatives thereof and salts thereof, vitamin E and its A whitening external preparation selected from derivatives and salts thereof, dibutylhydroxytoluene and butylhydroxyanisole.

The anti-inflammatory agent of component (B) is glycyrrhizic acid, glycyrrhetinic acid, mefenamic acid, phenylbutazone, indomethacin, ibuprofen, ketoprofen, allantoin, guaiazulene and derivatives thereof, and salts thereof, chondroitin sulfate and salts thereof, ε-aminocaprone Acid, diclofenac sodium, ashitaba extract, arnica extract, aloe extract, turmeric extract, hypericum extract, buckwheat extract, chamomile extract, goldfish extract, watercress extract, comfrey extract, salvia extract, Whitening topical preparation selected from sicon extract, perilla extract, birch extract, tea extract, milk scent extract, elder extract, sorghum extract, mugwort extract, mugwort extract and eucalyptus extract .

Component (B) UV inhibitors include paraaminobenzoic acid, ethyl paraaminobenzoate, glyceryl paraaminobenzoate, amyl N, N-dimethylparaaminobenzoate, N, N-dimethylparaaminobenzoic acid-2-ethylhexyl, salicylic acid-2 -Ethylhexyl, ethylene glycol salicylate, homomenthyl salicylate, 4-methoxycinnamate-2-ethylhexyl, 4-ethoxycinnamic acid ethoxyethyl, 4-methoxycinnamic acid potassium, 4,5-diisopropylcinnamic acid methyl, dipara Methoxycinnamic acid mono-2-ethylhexanoate glyceryl, 2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxybenzophenonesulfonic acid, sodium 2-hydroxy-4-methoxybenzophenonesulfonate, 2,2'- Jihyd Xi-4,4′-dimethoxybenzophenone, 2,2′-dihydroxy-4,4′-dimethoxybenzophenone-5-sulfonic acid sodium salt, 2,4-dihydroxybenzophenone, 2,2 ′, 4,4′-tetrahydroxy Selected from benzophenone, 2- (2-hydroxy-5-methylphenyl) -benzotriazole, urocanic acid, ethyl urocanate, 4-t-butyl-4'-methoxy-dibenzoylmethane, titanium oxide, zinc oxide and iron oxide A whitening external preparation is disclosed.

Japanese Patent Application Laid-Open No. 2001-122765 (Patent Document 8) includes hibiscus, aloe, daiou, yellow spirit, waurusushi, prolongation grass, sanshishi, potato plum, kudzu root, saiko, senkyu, sojutsu, thin cargo leaf, bukuryo, licorice, peonies, Pepper, semi-summer, toki, cinnamon, ten drugs, yellow ream, peony, gentian, pentaploid, assembly, ganoderma, hemp, jaundice, apricot kernel, psoriasis, taiso, oxon, cinnamon, turmeric, endurance winter, Kojitsu, ground yellow, Garlic, sage, oregano, rosemary, laurel, celery, thyme, tarragon, onion, nutmeg, mace, clove, wasabi, sabor, basil, chili, bean tea, black tea, green tea, bamboo leaf, coffee, too much, bee , Wormwood, bear sasa, wolfberry, jab cycad, shiitake mushroom, ginkgo, hijiki, igisu, kombu, amaze Any one of a group of various plant extracts extracted from each plant composed of sea turtles with water or a lower alcohol or a lower alcohol aqueous solution, and a compound group composed of tannic acid, 2-hydroxy-4-methylbenzophenone-5-sulfate An active oxygen scavenger characterized by containing one or more selected from the above group or both groups as an active ingredient. A cosmetic comprising the active oxygen scavenger as an active ingredient is disclosed.

JP-A-2001-140 (Patent Document 9) contains a fermented extract obtained by liquid culture of fermentation raw materials containing rice bran and soybeans with Bacillus subtilis, and beer yeast and / or hojicha extract. A composition for removing active oxygen. The fermented extract contains at least rice bran and soybeans, inoculated with Bacillus subtilis into a liquid medium adjusted to pH 7.5-10, cultured in an aerobic atmosphere, and the aqueous fermentation solution obtained by filtering the culture solution is frozen. The composition for removing active oxygen according to claim 1, which is dried. A composition for removing active oxygen, wherein Bacillus subtilis is Bacillus natto. A composition for removing active oxygen, wherein the fermented extract further comprises a green tea extract obtained by extracting green tea with water and / or ethanol.

A composition for removing active oxygen, wherein the ratio of the fermented extract and the green tea extract is fermented extract / green tea extract = 1 / 0.01 to 0.5 (weight ratio of dry matter). Inoculate Bacillus subtilis into a liquid medium containing at least rice bran and soybeans and adjusted to pH 7.5 to 10, cultured in an aerobic atmosphere, and filtered and filtered the culture solution to freeze-dry and ferment A method for producing a composition for removing active oxygen, characterized by comprising an extract and containing the beer yeast and / or hojicha extract in the fermented extract. A green tea extract obtained by extracting green tea with water and / or ethanol into a fermented aqueous solution or a fermented extract or a green tea extract obtained by drying the extract, the weight ratio of solid matter is fermented extract / green tea extract = 1 / The manufacturing method of the composition for active oxygen removal characterized by mixing so that it may become 0.01-0.5. An edible composition comprising the above-mentioned active oxygen removing composition is disclosed.

JP 2000-327550 A (Patent Document 10) includes the following components (A) and (B). (A) Cam-cam extract (B) A skin external preparation characterized by containing an active oxygen removal agent. The active oxygen scavenger is superoxide dismutase, mannitol, carotenoids, hydroquinone and derivatives thereof, bilirubin, cholesterol, tryptophan, histidine, quercetin, quercitrin, catechin and derivatives thereof, rutin and derivatives thereof, taurine, thiotaurine, phospholipid, Glabrizine, glabrene, liquiritin, isoliquiritin, vitamins A and their derivatives, vitamins B and their derivatives, vitamins C and their derivatives, vitamins D and their derivatives, vitamins E and their derivatives, glutathione and An external preparation for skin which is selected from the derivatives thereof, dibutylhydroxytoluene, butylhydroxyanisole, gallic acid and derivatives thereof. A skin external preparation, wherein the content of the active oxygen scavenger is 0.0001 to 5% by weight.

Reactive oxygen scavenger is eggshell membrane extract, placenta extract, stevia extract, licorice extract, yeast extract, ogon extract, seaweed extract, tormentilla extract, rahan fruit extract, ginkgo extract, ganoderma extract , Grape extract, caquette extract, tea extract, strawberry tea extract, peony extract, gentian extract, hawthorn extract, rosemary extract, mokka extract, yukinoshita extract, yashajitsu extract, geno shoko extract , A topical skin preparation selected from botpi extract, Melissa extract, parsley extract, dicoppi extract, lettuce extract, cucumber extract, bifidobacteria metabolite. A skin external preparation wherein the content of the active oxygen removing agent is 0.0001 to 5% by weight as a dry solid content. The skin external preparation whose content of a cam cam extract is 0.0001 to 5 weight% as dry solid content is disclosed.

Japanese Unexamined Patent Publication No. 2000-229828 (Patent Document 11) contains a tomato juice as an active ingredient, and a skin whitening external preparation for whitening. Furthermore, the skin external preparation for whitening characterized by containing 1 type, or 2 or more types of the medicinal component selected from the group which consists of a whitening agent, an active-oxygen removal agent, an antioxidant, an anti-inflammatory agent, and an ultraviolet-ray inhibitor. For whitening, the whitening agent is one or more selected from the group consisting of grabrizine, glabrene, liquiritin, isoliquiritin, hydroquinone and derivatives thereof, cysteine and derivatives thereof, vitamin C and derivatives thereof, and glutathione and derivatives thereof. Skin external preparation.

Whitening agent is placenta extract, Sempukuka extract, keiketou extract, licorice extract, sunpens extract, Sakuhakuhi extract, Toki extract, Ibukitorano extract, Clara extract, hawthorn extract, white lily extract, hop It is selected from the group consisting of an extract, an age extract, a squid cuttlefish extract, a gokahi extract, a mokka extract, a brown sugar extract, a ginseng extract, a yakuinin extract, a seaweed extract, a saicin extract and a rice fermentation extract Skin external preparation for whitening. The active oxygen scavenger comprises superoxide dismutase (SOD), mannitol, carotenoids, astaxanthin, rutin and derivatives thereof, bilirubin, cholesterol, tryptophan, histidine, quercetin, quercitrin, catechin and derivatives thereof, and gallic acid and derivatives thereof. A skin external preparation for whitening, which is one or more selected from the group.

Active oxygen scavengers are: Ogon extract, Ginkgo biloba extract, Yukinoshita extract, Melissa extract, Gennoshoko extract, Botpi extract, Parsley extract, Tormentilla extract, Rakan fruit extract, Yashajitsu extract, Zicopi extract, The whitening skin external preparation according to claim 2, which is one or more selected from the group consisting of rosemary extract, peony extract, grape seed extract, stevia extract and yeast extract.

The antioxidant is selected from the group consisting of vitamins A and their derivatives, vitamins B and their derivatives, vitamins D and their derivatives, vitamins E and their derivatives, dibutylhydroxytoluene and butylhydroxyanisole The skin external preparation for whitening which is 1 type or 2 types or more is disclosed.

Japanese Unexamined Patent Publication No. 2000-229827 (Patent Document 12) contains a tomato pigment as an active ingredient. A skin external preparation whose tomato pigment is mainly composed of a lycopene complex obtained by centrifuging a processed tomato product, collecting a liquid portion, and microfiltrating this to collect a non-permeate. Furthermore, the added active oxygen scavenger is a hornon extract, a ginkgo biloba extract, a quette extract, a hawthorn extract, a mica mosquito extract, a cypress extract, a melissa extract, a genus shochu extract, a button pi extract, a parsley extract, A skin external preparation is disclosed that is one or more selected from the group consisting of Tormentilla extract, Rakan fruit extract, Yashajitsu extract, Dicoppi extract, Stevia extract and Agetsu extract.

JP 2000-210262 (Patent Document 13) discloses an external preparation for skin containing ((A) an acerola extract and (B) an active oxygen remover. Oxide dismutase, mannitol, carotenoids and animal and plant extracts containing these, hydroquinone and derivatives thereof, and salts thereof, bilirubin, cholesterol, tryptophan, histidine, quercetin, quercitrin, catechin and derivatives thereof, rutin and derivatives thereof, taurine, Thiotaurine, soybean and egg-derived phospholipids, eggshell membrane extract, placenta extract, gallic acid and its derivatives, stevia extract, grabrizine, glabrene, liquiritin, isoliquiritin and licorice extract containing these, yeast extract, ougone Extract Seaweed extract, Tormentilla extract, Rahan fruit extract, Ginkgo biloba extract, Ganoderma extract, Grape seed extract, Caquette extract, Tea extract, Peonies extract, Gentian extract, Hawthorn extract, Rosemary extract , Mokka extract, Yukinoshita extract, Yashajitsu extract, Gennoshoko extract, Boppi extract, Melissa extract, Parsley extract, Zicopi extract, vitamin A and their derivatives and their salts, vitamin B and their Derivatives thereof and salts thereof, vitamin Cs and derivatives thereof, and salts thereof, vitamin Ds and derivatives thereof, and salts thereof, vitamin Es and derivatives thereof, and salts thereof, glutathione and derivatives thereof and salts thereof, From dibutylhydroxytoluene and butylhydroxyanisole Skin external preparation is disclosed in which a Barre.

JP 2000-128762 A (Patent Document 14) discloses that active oxygen scavengers include carotenoids such as superoxide dismutase (SOD), mannitol, and beta-carotene, astaxanthin, rutin and derivatives thereof, bilirubin, cholesterol, tryptophan, Histidine, quercetin, quercitrin, catechin, catechin derivatives, gallic acid, gallic acid derivatives, hornon extract, ginkgo biloba extract, saxifrage extract, melissa extract, gentian pepper extract, button pi extract, parsley extract, tormentilla extract In addition, skin external preparations for skin whitening, which are one or more selected from the group consisting of a rakan fruit extract, a seaweed extract, a yashajitsu extract and a dicoppi extract, are disclosed.

Japanese Patent Application Laid-Open No. 11-292785 (Patent Document 15) discloses a mycelium extract of Agaricus blazei Murill and Agaricus blazei.
Murill) A topical skin preparation comprising one or more selected from mycelium culture filtrate and an active oxygen scavenger. A skin external preparation, wherein the active oxygen scavenger is a plant extract having an active oxygen scavenging action. An external preparation for skin, wherein the active oxygen scavenger is a plant extract containing one or more components selected from carotenoids, flavonoids, and tannins. The active oxygen scavenger is Hamamelis japonica Sieb.et Zucc.
, Hamamelis obtsusata Makino, Hamamelis japonica
Sieb.et Zucc.var. Obtsusata Matsum., Hamamelis virginiana
L., Hamamelis mollis Oliv.), Quercus genus, Carcinus genus, Burraceae genus, Button genus, Ginkgo bibloba L., Birch genus, Parsley ( Petroselium saticum , Petroselium sativm Hoffm., Petroselium
crispum Nyman, Petroselium hortense Hoffm., Petroselium
A topical skin preparation characterized by being an extract of one or more plants selected from crispum (Mill.) Nym. ex AW Hill, Apium petroselium L.) is disclosed.

An external preparation for skin characterized in that the active oxygen scavenger is hamamelitannin. A skin external preparation, wherein the active oxygen scavenger is one or more components selected from carotenoids, flavonoids, and tannins. A skin external preparation, wherein the active oxygen scavenger is one or more components selected from superoxide dismutase, gallic acid and salts or derivatives thereof, hydroquinone and salts or derivatives thereof. A skin external preparation, wherein the active oxygen scavenger is one or more components of thioredoxin, thioredoxin reductase, and a complex thereof. Disclosed is an external preparation for skin characterized in that the active oxygen scavenger comprises one or more components of thioredoxin, thioredoxin reductase and their complexes, and reduced nicotinamide adenine dinucleotide phosphate ing.

Japanese Patent Application Laid-Open No. 11-292784 (Patent Document 16) discloses an external preparation for skin comprising an extract of a fruit body of Agaricus blazei Murill and an active oxygen scavenger. A skin external preparation, wherein the active oxygen scavenger is a plant extract having an active oxygen scavenging action. The topical skin preparation according to claim 1, wherein the active oxygen scavenger is a plant extract containing one or more components selected from carotenoids, flavonoids, and tannins. The active oxygen scavenger is Hamamelis japonica Sieb.et Zucc.
, Hamamelis obtsusata Makino, Hamamelis japonica
Sieb.et Zucc.var. Obtsusata Matsum., Hamamelis virginiana
L., Hamamelis mollis Oliv.), Quercus genus, Carcinus genus, Burraceae genus, Button genus, Ginkgo bibloba L., Birch genus, Parsley ( Petroselium saticum , Petroselium sativm Hoffm., Petroselium
crispum Nyman, Petroselium hortense Hoffm., Petroselium
A topical skin preparation characterized by being an extract of one or more plants selected from crispum (Mill.) Nym. ex AW Hill, Apium petroselium L.) is disclosed.

A skin external preparation, wherein the active oxygen scavenger is one or more components selected from carotenoids, flavonoids, and tannins. An external preparation for skin characterized in that the active oxygen scavenger is hamamelitannin. A skin external preparation, wherein the active oxygen scavenger is one or more components selected from superoxide dismutase, gallic acid and salts or derivatives thereof, hydroquinone and salts or derivatives thereof. A skin external preparation, wherein the active oxygen scavenger is one or more components of thioredoxin, thioredoxin reductase, and a complex thereof. Disclosed is a topical skin preparation characterized in that the active oxygen scavenger comprises thioredoxin, thioredoxin reductase and one or more components of these complexes, and reduced nicotinamide adenine dinucleotide phosphate. ing.

Japanese Patent Application Laid-Open No. 8-3551 (Patent Document 17) discloses an antioxidant and an active oxygen scavenger derived from saccharum. An antioxidant and an active oxygen scavenger containing an active ingredient obtained by extracting sensual grass with water or a hydrophilic organic solvent are disclosed.

Japanese Patent Laid-Open No. 6-24937 (Patent Document 18) includes Ryutan, Kyokuyo, Hibiscus, Aloe, Daio, Yellow spirit, Owaurushi, Life-saving grass, Sanshishi, Satsuma plum, Kakkon, Psycho, Senkyu, Thin-leaved leaf, Bukkuri, Licorice, Peonies, Yokuinin, Spicy, Semi-summer, Toki, Cinnamon, Yellow-legged, Peony, Gentiana, Peony, Pyramid, Assembly, Ganoderma, Mao, Jaundice, Ogon, Chen, Turmeric, Goshyu, Sage, Oregano, Rosemary, Laurel , Tarragon, onion, nutmeg, clove, wasabi, sabori, basil, chili, bean tea, black tea, green tea, bamboo leaf, coffee, too, bee, wormwood, kumasasa, wolfberry, yabusotetsu, shiitake Various plants extracted with water, lower alcohol or lower alcohol aqueous solution Extracts, and L-cysteine, glutathione, mannitol, gallic acid, potassium sorbate, acetylacetone, triethanolamine, tannic acid, 4-t-butyl-4'-methoxy-dibenzomethane, 2-hydroxy-4-methyl A mucopolysaccharide fragmentation inhibitor comprising, as an active ingredient, one or more selected from one or both of a group of compounds consisting of benzophenone-5-sulfate. Hibiscus, Aloe, Daio, Yellow spirit, Walrus, Prolonged grass, Sanshishi, Japanese apricot bark, Kudzu root, Psycho, Nematode, Soujutsu, Thin-leafed leaf, Bakuryo, Licorice, Peonies, Hot pepper, Half-summer, Toki, Keiko, Tenyaku, Huangren , Peony skin, gentian, pentaploid, assembly, ganoderma, mahuang, jaundice, apricot kernel, psoriasis, lysins, dragonfly, cinnamon, turmeric, cypress, cypress, ground yellow, garlic, sage, oregano, rosemary, laurel, celery, thyme, Tarragon, onion, nutmeg, mace, clove, wasabi, sabori, basil, chili, bean tea, black tea, green tea, bamboo leaf, coffee, too, bee, wormwood, kumasasa, wolfberry, yabusotetsu, shiitake, ginkgo, hijiki, Water or lower arco from each plant consisting of Igisu, Kombu, Arame, Oniwakame Selected from one or both of a group of various plant extracts extracted with an aqueous solution of alcohol or lower alcohol and a compound group consisting of tannic acid, 2-hydroxy-4-methylbenzophenone-5-sulfate An active oxygen scavenger containing one or more active ingredients as an active ingredient is disclosed.

Japanese Patent Publication No. 8-40 (Patent Document 19) is inoculated with Bacillus natto or Bacillus subtilis in a liquid medium containing rice bran, soybeans and water and adjusted to pH 7.5 to 10 with an alkaline agent. A water-soluble green tea extract extracted by culturing in an aerobic atmosphere and extracting the fermentation aqueous solution produced by filtration or the evaporation residue of the fermentation aqueous solution with a solvent containing at least one of water and ethanol Or the active-oxygen suppression composition characterized by including the evaporation residue of this water-soluble green tea extract. Inoculated with Bacillus natto or Bacillus subtilis into a liquid medium containing rice bran, soybeans and water, adjusted to pH 7.5-10 with an alkaline agent, cultured in an aerobic atmosphere, filtered and fermented aqueous solution A water-soluble green tea extract extracted by extraction with a solvent containing at least one of water and ethanol is added to the aqueous fermentation solution to produce an aqueous active oxygen suppression composition. The manufacturing method of the active oxygen suppression composition characterized by this is disclosed.

However, it can be said that these are expensive and are not always satisfactory in their effects.
JP 2003-81744 A JP 2003-12538 A JP 2002-275018 A JP 2002-179581 A JP 2002-173441 A JP 2001-199868 A JP 2001-181173 A JP 2001-122765 A Japanese Patent Laid-Open No. 2001-140 JP 2000-327550 A JP 2000-229828 A JP 2000-229827 A JP 2000-210262 A JP 2000-128762 A JP 11-292785 A JP 11-292784 A JP-A-8-3551 JP-A-6-24937 Japanese Patent Publication No. 8-40

  The present invention is intended to provide a composition having a function of removing active oxygen that causes various diseases such as cancer, arteriosclerosis, diabetes, aging, atopic dermatitis, allergic diseases when accumulated in a large amount in the body. In particular, it is used for cosmetics and food and drink applications.

There are about 220 species of rush (Juncus spp.) Distributed throughout the world. It is cultivated mainly in Japan, China, and Southeast Asia because it is easy to grow in areas with relatively high humidity. The genus Juncus cultivated is used around the world as a rug and mop. In Japan, the plants of the genus Juncus are called “rush” and have been used for more than 600 years as judo mats and indoor carpets.

The rush has no history and has been used as a medicinal herb for diuretics and anti-inflammatory drugs since ancient times. In the clinical practice and application of Chinese medicine, it is said that 1.5 to 3 g should be taken once as a diuretic or as a treatment for edema. Furthermore, it is written that the new Japanese traditional Chinese medicine improves symptoms by applying a bite of rush to cuts, bleeding, and bruises. The rush has been shown to have antibacterial activity against enterohemorrhagic Escherichia coli O157. In addition, it had antibacterial activity against enterohemorrhagic Escherichia coli O26 and O111, Salmonella with the highest number of food poisoning, Staphylococcus aureus that became a problem due to milk contamination, and Bacillus subtilis and Micrococcus that were spoilage bacteria. . Antibacterial components are also resistant to acids and heat. For this reason, when rush is eaten, even if it passes through a stomach with a strong acidity, the antibacterial property is not lost, and it quickly reaches the intestines.

However, as “reactive oxygen” research has progressed in recent years, the “toxicity” of oxygen has become apparent. Oxygen literally oxidizes anything. Oxygen affects the iron rusting and the cut apples becoming black. Reactive oxygen has an oxidizing power approximately 1500 times that of oxygen and exerts an immune effect in small amounts, but when accumulated in the body in large quantities, cancer, arteriosclerosis, diabetes, aging, atopic dermatitis, allergic diseases It is known to cause various diseases. It is said that about 90% of diseases are caused by active oxygen. Active oxygen accumulates in the body by drinking alcohol and tobacco, accumulating stress, living in a dirty environment, and eating foods rich in food additives. However, these are unavoidable in modern life, and it is necessary to eat foods that eliminate active oxygen on a daily basis.

The rush is a plant excellent in scavenging active oxygen, and it was revealed that 4200 units of scavenging active oxygen per gram of rush. This value is about five times as high as kale, the raw material of green juice, which is also famous for eliminating active oxygen. Rabbits have as much as 63g of dietary fiber per 100g. This is quite high among various crops, about 40 times that of lettuce, about 9 times that of sweet potato, and about twice that of spinach. It has already been clarified that dietary fiber has many effects such as cholesterol suppression effect, blood sugar level increase suppression effect, colon cancer occurrence suppression effect.

By the way, when screening Bacillus subtilis from rice straw, it has been known for a long time that about 30 to 35 bacteria are separated. These bacteria are considered to be related to Bacillus natto (Bacillus subtilis). There have been many traditional fermented foods using microorganisms in Japan since ancient times, but Itobiki Natto is one of them, and it is unique that it is rich in nutrients that are safe and medicinal. It is a salt-free fermented food. The safety system, medicinal properties, and nutrients are preserved in natural natto bacteria (a kind of Bacillus subtilis) that survive in the natural world, organically grown soybeans harvested from the land of nature, and natural without long-term improvement for a thousand years. This is because it has been made by the fermenting technology. Since ancient times, Itobiki Natto has been described in the old book `` this breakfast book '' as a medical food source, and it seems that it was often eaten to prevent infectious diseases, especially dysentery, typhoid, cold, used as a folk remedy, In recent years, it has been used as a soft-hearted syringo, a bamboo shoot of dermatosis, and it is said that it is effective in preventing hypertension, cerebral thrombosis, and myocardial candles. As a result of research, it has been found that the thread (stickiness) of Itobiki Natto has a medicinal effect on osteoporosis.

Vitamin K contained in the thread (stickiness) plays a major role in increasing calcium in the body. It is also found that it contributes to mental stability, and even if natto rice is swallowed, the thread (stickiness) contains an enzyme called cellulase, so it has the power to help digestion, and in addition to mine cancer In addition to sexual immunity, the research announcement of the stickiness (thread) of Itobiki Natto for food poisoning of 0-157 has an antibacterial effect on pathogenic Escherichia coli has become a hot topic. All of these medicinal Itobiki natto must be of good quality.

At the same time as Bacillus natto, which becomes a good bacteria and kills bad bacteria, we will increase other good bacteria such as bifidobacteria. Such power is in natto bacteria. The ingested natto bacteria are very stable because they are in the form of spores, and even if they encounter stomach acid, they do not die easily. The fungus passes through the stomach, some divides and propagates along the way, and some reaches the intestine in the form of spores. It is said that the number is in hundreds of millions. In the intestines, there are various intestinal bacteria ranging from good bacteria to bad bacteria, and they interact to produce various enzymes and clean the intestines. Natto bacteria, in cooperation with these good bacteria, create a special enzyme, which kills spoilage bacteria caused by constipation and diarrhea and increases the good bacteria. This is the currently estimated mechanism of the intestinal action of Bacillus natto.

Speaking of enzymes, the enzymatic action of Bacillus natto has already been put into practical use in medicine, and digestive agents have been purified. Recently, the words “functionality of food” and “functional food” have come to be used a lot, but if we eat the primary function that is a nutrient necessary for living the food we are eating. It is named as a tertiary function because it has a secondary function that appeals to the sense that it looks delicious, and another “biological regulation function”. The third function is to improve our immunity, control our body's work, such as prevention and recovery of illness and prevention of aging, and maintain a healthy body. Natto, a fermented food, seems to have a high degree of tertiary function in addition to nutritious soy and appetizing natto. Fermented food natto, which is made from the protein of beans that can cause diarrhea when eaten raw, has been transformed into a form that can be eaten with the help of microorganisms. There are various factors that increase health and longevity, but dietary habits are the factors that can ensure health through personal attention. Natto is a fermented food that is rich in nutrients and excellent in regulating the intestines.

There are also the following experiments by Dr. Kameda. “When we injected natto bacteria into only one of the legs of a mouse that had cancer cells implanted at the base of both feet and observed the development of the subsequent cancer, there was cancer in the legs that did not receive natto bacteria. However, there was no cancer if injected, and it was less than half the probability, if any. ”This was a big topic when it was announced in the newspaper. There is an unknown part.

In addition, in order to test the medical effects of natto bacillus spores, we selected rats in a pedigree that is prone to hypertension, and gave them salinity that could be said to be the source of hypertension. There is an experiment in which these rats were divided into two groups while making the substrate. An experiment was conducted in which natto was given to one and nothing was given to the other. As a result, rats given natto were less likely to develop hypertension and had a longer life, whereas rats not given natto became hypertension and had a shorter life. Based on the results, we investigated what was effective in natto and found that the spore of natto has a strong anti-blood pressure-preventing ability.

There is an experiment report that it is an experiment by a mouse, but when the ingredient contained in natto was given to a rat that had chronic pancreatitis, it was improved. The discovery of the thrombolytic enzyme “nattokinase” has recently attracted much attention to the hidden power of Bacillus natto. This is the ultimate ingredient born from the encounter between natto bacteria and soybeans. This is because nattokinase is only born when soybeans and natto bacteria meet. It is contained in the unique stickiness of natto, which was born as a result of fermenting soybeans by the action of natto bacteria.

By the way, most of the sticky ingredients of natto are one of the amino acids called γ-glutamic acid and one of the umami ingredients. This nattokinase works to prevent thrombus formation and dissolve the thrombus. Mr. Hiroyuki Sumi, Okayama Prefectural College, researched the effects of natto ingredients by placing natto on an artificially created thrombus (blood clot) and keeping it at 37 ° C, the same as body temperature. I found that the thrombus was brilliantly dissolved. After exploring the causative component, I arrived at an enzyme in natto's gooeba and decided to call it "nattokinase" after the name of natto. One component in natto was the cause of death. It prevents the thrombus causing the myocardial candle, cerebral thrombus, stroke, etc., which occupy a high ratio, and dissolves the thrombus. There is a possibility that it can prevent the sequelae and blurs of these diseases, which are now a major social problem.

The component secreted into the human body to prevent blood clots is an enzyme called plasmin, and synthesis of this enzyme called plasmin requires a substance released into the blood from cells inside the blood vessel wall. It is said that thrombosis is caused by the lack of the substance. Nattokinase seems to have a great connection with the substance, but that is the only thing that is known. Probably nattokinase is presumed to work to promote the secretion of the substance, but it is not clear. Anyway, if the function of nattokinase is elucidated, it seems to exert great power in preventing adult diseases, reducing mortality, and preventing blur.

Of course, if you eat natto, you can take this nattokinase naturally. According to animal experiments, this nattokinase is said to continue to be active even in the stomach and intestines. A blood clot is a litter-like lump in a blood vessel that literally plugs the blood vessel or obstructs the flow of blood. Even die. If it occurs in the heart, it becomes “myocardial infarction”, and if it occurs in the cerebral blood vessel, it becomes “cerebral infarction”. In contrast to this sudden and severe suffering, the microvascular flow gradually deteriorates and the brain cells in the basin are weakened unconsciously. Fibrin is a protein formed by fibrinogen, which is always contained in a large excess in blood, by the action of a plurality of enzymes called coagulation system called platelets and blood coagulation system.

Nattokinase is a powerful enzyme similar to plasmin in the blood, synthesized by Bacillus natto, and not contained in products such as soybeans or tofu. Animal experiments have confirmed that nattokinase passes through the stomach (acidic), does not lose activity in the intestine (alkaline), and even when given orally, it dissolves thrombus in blood vessels. Considering these factors, it is said that if natto is eaten on a daily basis, the effect of increasing the thrombolytic activity of blood can be sufficiently expected. In fact, these blood clots are always made in our bodies. This is because blood vessels are always at risk of injury and bleeding due to various causes, and thrombus is necessary to prevent this. In other words, a thrombus is not unilaterally bad. The living body repairs a blood vessel while the thrombus is formed, and after that, the used thrombus is immediately dissolved. is there. And this fibrin (thrombus) is dissolved by an enzyme group called fibrinolytic system, and this activity should be balanced with the activity of the previous coagulation system. Problems arise.

Now, we paid attention to the nutrients of plankton, of which zooplankton, especially krill. Phytoplankton varies from primitive algae that are less than 1 micron (thousandth of a millimeter) to large diatoms that are over 1 mm. On the other hand, zooplankton comes in various shapes and sizes, and you eat phytoplankton in a way that suits your life. Crustacean krills (several centimeters) and copepods (several millimeters) are eaten by rubbing and catching large algae (tens to hundreds of microns) using the legs around the mouth. Very small zooplankton (tens to hundreds of microns) like protozoa feed on even smaller algae and bacteria. Others, such as the Otamaboya, eat only small plankton using a special sieve to sort food.

There are currently 85 types of krill classified around the world. “Kiki” in “krill” means “offshore” and is widely distributed from the coast of the ocean to the offshore waters. The krill belongs to the crustacean upper class, the soft crustacean, the true soft shell subclass, the king shrimp, the krill, and the appearance is similar to the mysids and shrimps, Different animal groups. Krills, like other crustaceans, become adults after undergoing a molting stage that undergoes molting and different shapes. First, eggs are laid and hatched to become Nauplius larvae.

This nauplius larva has 1st and 2nd stages, and then becomes metanapurius larvae. Methanopius larvae molt and become shrimp-type calyptopis larvae. The calyptopis larvae period consists of three stages. During this period, the body is basically divided into two parts, the mouth organs are completed and food can be taken. The following Farsi larvae are in six stages, during which the abdominal legs and chest legs develop, spiders appear, and the development of light emitters, tail joints, and compound eyes are also seen. When juvenile is complete, all legs are completed, the tail is clear, and the figure is an adult. However, the gonad is underdeveloped, no secondary sexual characteristics are seen, and male and female discrimination is difficult. In the case of Antarctic krill, the eggs that have been laid down on the surface layer will sink, and the stage until hatching will proceed, and will hatch at a depth of several thousand meters. As hatched nauplius larvae, metanoprius larvae, and as the stage of development progresses, they gradually rise to the upper layers, and calyptopis larvae that can feed, phytoplankton, etc. It will come to near the surface where there is. If eggs arrive on the continental shelf, etc., at a depth of about 500 meters, the eggs will develop on the spot, and the larvae will develop near the bottom of the continental shelf, and hatched at a depth of several thousand meters. As with, it rises near the surface when it can be eaten.

  In general, krills feed on phytoplankton during the larval stage. Juvenile and adults are slightly different depending on the type and habitat, but feeding on the chest leg is fundamental. The most common feeding method is filtered feeding. This is a method of spreading chest legs and filtering phytoplankton and small zooplankton in seawater. There is also a method of catching and eating medium-sized zooplankton using the chest legs. There are various feeding patterns, such as scraping phytoplankton under the ice using the chest legs, catching organisms in the mud, and feeding detritus in the sea.

In this way, krills eat phytoplankton, and the krill feeds fish and the like, and there are abundant nutrients inside the living body. In other words, zooplankton containing EPA (and DHA) is eaten by zooplankton, small fish such as sardine feed on it, and large migratory fish such as tuna eat sardine and so on. The main action of EPA is to prevent blood coagulation, improve blood flow (blood smoothing effect), reduce bad cholesterol, and increase good cholesterol. Effective in preventing and improving lifestyle-related diseases such as sclerosis, myocardial infarction, cerebral infarction, hyperlipidemia, hypertension, obesity, etc. Prevents blood coagulation and improves blood flow (blood smoothness effect)-Reduces bad cholesterol, increases good cholesterol, but prevents oxidation If you don't take it, it will oxidize immediately and you will not be able to exert its power.There are EPA and DHA with many excellent actions including "blood smoothness effect", but there are actually weak points. Oxidized EPA, DHA increases the active oxygen in the body, so it works negatively for the body, so it is desirable to use it together with vitamin E and vitamin C, which are powerful antioxidants. .

  This inventor provides the biological function regulator which has an active oxygen removal function which makes the following structure a requirement, and the composition for cosmetics and food-drinks containing it. Namely, a biological function regulator having a function of removing active oxygen, comprising a fermented liquid obtained by fermenting soybean extract, rush extract and krill extract with Bacillus natto, and a cosmetic composition containing the same It is a composition for food and drinks.

ADVANTAGE OF THE INVENTION According to this invention, the biological function regulator which has an active oxygen removal function, and the composition for cosmetics and food-drinks containing it can be provided, it has the function to erase | eliminate active oxygen, Fiber has many effects such as cholesterol suppression effect, blood sugar level increase suppression effect, colon cancer occurrence suppression effect, prevents blood coagulation, improves blood flow (blood smoothness effect) reduces bad cholesterol, improves good cholesterol increase. As a result, it is effective in preventing and improving lifestyle-related diseases such as arteriosclerosis, myocardial infarction, cerebral infarction, hyperlipidemia, hypertension and obesity. Prevent blood coagulation and improve blood flow (blood smoothness effect) Reduce bad cholesterol and increase good cholesterol. As a result, it is effective in preventing and improving lifestyle-related diseases such as arteriosclerosis, myocardial infarction, cerebral infarction, hyperlipidemia, hypertension and obesity.

  A biological function regulator having an active oxygen removing function, a cosmetic composition containing the same, and a food and drink comprising a fermented liquid obtained by fermenting soybean extract, rush extract and krill extract with natto bacteria A product composition is obtained.

EXAMPLES The present invention will be described more specifically with reference to examples. However, the present invention is not limited to the examples.
[Preparation of rush extract]
Put 100g of dried and crushed raw material in 10 times amount of hot water at 60 ° C, extract for one day and night, centrifuge and separate into extract and residue. Add about 5 times the amount of hot water at 100 ° C again. The mixture was extracted at 60 ° C. and extracted at 100 ° C., and the mixture was extracted into an rush extract. After filtering this extract at about 50 ° C., After cooling to 15 ° C. again, filtration was performed, followed by concentration under reduced pressure, further freeze-drying, and a solution dissolved in 10 ml of distilled water was prepared as an extract.

[Preparation of soybean extract]
Put 100g of dried and crushed raw material in 10 times amount of hot water at 60 ° C, extract for one day and night, centrifuge and separate into extract and residue. Add about 5 times the amount of hot water at 100 ° C again. After one day and night extraction, after centrifugation, a mixture of the extract extracted at 60 ° C. and the extract extracted at 100 ° C. was used as a soybean extract, and this extract was filtered at about 50 ° C. After cooling to 15 ° C. again, filtration was performed, followed by concentration under reduced pressure, further freeze-drying, and a solution dissolved in 10 ml of distilled water was prepared as an extract.
[Preparation of krill extract]
After putting 100g of dried and crushed raw material in 60 times hot water of 60 ° C, and extracting for one day and night,
After centrifuging, the extract is separated from the residue, and the residue is extracted again by adding about 5 times the amount of 100 ° C hot water for one day and night, and after centrifugation, the extract extracted at 60 ° C and the extract extracted at 100 ° C A mixture of the liquids is used as a krill extract, and the extract is filtered at about 50 ° C., then cooled again to 15 ° C., filtered, and then concentrated under reduced pressure, followed by lyophilization, Then, it prepared as a 10 ml extract.
[Fermentation with Bacillus natto]
Mix the same amount of rush extract, soybean extract and krill extract (3.3 ml each) and dilute to 1 L with water, then inoculate natto and inoculate under aerobic conditions. What was emitted at 24 ° C. for 24 hours and aged for 24 hours in a refrigerator at 10 ° C. was filtered again at 10 ° C., and then concentrated to 10 ml under reduced pressure to obtain a fermentation solution of Bacillus natto. In addition, in the case of each independent extract, after diluting 10 ml each in 1 L of water and fermenting similarly, the extract was obtained as described above.

  Superoxide elimination test (NBT method) The extract obtained by the above preparation method was tested for superoxide elimination by the following test method.

3 mM xanthine, 0.05M
Na ₂ CO ₃ buffer solution (pH 10.2), ₃ mM EDTA, BSA solution and 0.75 mM NBT 0.1 mL each were placed in a test tube, 0.1 mL of the sample solution was added thereto, and the mixture was allowed to stand at 25 ° C. for 10 minutes. Subsequently, the xanthine oxidase solution was added, it stirred rapidly, and it left still at 25 degreeC for 20 minutes. Thereafter, 0.1 mL of 6 mM copper chloride was added to stop the reaction, and the absorbance at a wavelength of 560 nm was measured. The absorbance measured at this time is referred to as “absorbance upon addition of enzyme solution and sample solution”.

The same operation and measurement of absorbance were performed without adding the enzyme solution. The absorbance measured at this time is referred to as “absorbance when no enzyme solution is added and the sample solution is added”.

The same measurement was performed when distilled water was added without adding the sample solution. The absorbance measured at this time is referred to as “absorbance when the enzyme solution is added and the sample solution is not added”.

The same measurement was performed when distilled water was added without adding the enzyme solution and without adding the sample solution. The absorbance measured at this time is referred to as “absorbance when no enzyme solution is added and no sample solution is added”. And superoxide elimination rate (%) was calculated | required by following Formula.

Erase rate (%) = {1- (A−B) / (C−D)} × 100 In the formula, “A” represents “enzyme solution addition, absorbance at the time of sample solution addition”, and “B” represents “enzyme solution” Absorbance without addition, sample solution added, “C” represents “absorbance without addition of enzyme solution, no sample solution”, and “D” represents “absorbance without addition of enzyme solution, no sample solution”.

The above-mentioned erasure rate was measured by gradually reducing the sample concentration, and the sample concentration (ppm; μg / mL) at which the superoxide erasure rate was 50% was determined by interpolation. The results are shown in Table 1.

Table 1
Liquid sample fermented with Bacillus natto
50% erased sample concentration Extract of rush 163.5
2. Krill extract 154.2
3. Soybean extract 162.6
4). Rush extract, krill extract and soybean extract 70.6
From the results shown in Table 1, it was confirmed that an extract from a solution obtained by fermenting a rush extract, a krill extract, and a soybean extract with Bacillus natto has a remarkable superoxide scavenging action. It was also confirmed that the degree of superoxide scavenging action can be adjusted by the concentration of the extract. (Hereafter, a mixture of rush extract, krill extract and soybean extract fermented with Bacillus natto is called "natto fermented liquor")

[Formulation Example 1] An emulsion having the following composition was produced by a conventional method.
Jojoba oil 4.0g
Placenta extract 0.1g
Ascorbic acid magnesium phosphate 0.1g
Prune extract 1.0g
Tounin extract 1.0g
Kyonin extract 1.0g
Taio extract 0.5g
Cousin extract 0.5g
Olive oil 2.0g
Squalane 2.0g
Cetanol 2.0g
Glyceryl monostearate 2.0g
Polyoxyethylene cetyl ether 2.5g
Oleic acid polyoxyethylene sorbimine 2.0g
1,3-butylene glycol 3.0 g
Hinokitiol 0.15g
Natto fermentation broth 3.0g
Purified water balance (total amount is 100 g)

[Formulation Example 2] A cream having the following composition was produced by a conventional method.
Liquid paraffin 5.0g
Salami beeswax 4.0g
Cetanol 3.0g
Squalane 10.0g
Lanolin 2.0g
Arbutin 0.1g
Lucinol 0.1g
Kojic acid 0.1g
Twilight extract 0.1g
Ginkgo biloba extract 0.1g
Conchiolin 0.1g
Oat extract 0.1g
Stearic acid 1.0g
Oleic acid polyoxyethylene sorbitan 1.5g
3.0 g glyceryl monostearate
1,3-butylene glycol 6.0 g
0.05 g of methyl paraoxybenzoate
Fragrance 0.1g
Natto fermentation broth 5.0g
Purified water balance (total amount is 100 g)

[Composition Example 3] A pack having the following composition was produced by a conventional method.
Polyvinyl alcohol 15.0g
Polyethylene glycol 3.0g
Propylene glycol 7.0g
Ethanol 10.0g
Chamomile extract 0.1g
Ellagic acid 0.1g
Natto fermentation broth 3.0g
Oil soluble licorice extract 0.1g
Seaweed extract 0.1g
Yeast extract 0.1g
Perilla extract 0.1g
Juju extract 0.1g
Linden extract 0.1g
0.05 g ethyl paraoxybenzoate
Fragrance 0.05g
Purified water balance (total amount is 100 g)

[Formulation Example 4] A capsule having the following composition was produced by a conventional method. As the capsule, No. 1 hard gelatin capsule was used.
Dried Bacillus natto fermentation solution ... 100.0mg
Cornstarch ... 60.0mg
Lactose ... 100.0mg
Calcium lactate ... 10.0mg
Hydroxypropylcellulose (HPC-L) ... 10.0mg

[Formulation Example 5] A granular preparation having the following composition was produced by a conventional method.
Dried natto fermentation liquid ... 250.0mg
Bone meal (containing 20% calcium) ... 250.0mg
Eggshell calcium (containing 34% calcium) ... 147.0mg
Vitamin B group mixed powder (including all vitamin B groups) 100.0mg
Fat-soluble vitamin mixed powder (including vitamins D, K, E, A) ... 100.0mg
Milk protein hydrolyzate (including amino acids and low molecular weight peptides) 50.0mg
Corn / soy protein hydrolyzate (including amino acids and peptides) ... 20.0mg
Wheat germ extract (including vitamins, chromium, selenium, molybdenum) ... 30.0mg
Yeast extract (including vitamins, nucleic acids and zinc) ... 50.0mg
Erythritol ... 300.0mg
Lactulose ... 100.0mg
Maltitol ... 168.7mg
Beet oligosaccharide ... 100.0mg
Whey oligosaccharides ... 100.0mg
Guar gum ... 60.0mg
Glycerin fatty acid ester 15.0mg

[Composition Example 6] An oral liquid preparation having the following composition was produced by a conventional method.
Natto fermentation broth 0.5g
Sorbit 12.0g
Sodium benzoate 0.1g
Fragrance 1.0g
Calcium sulfate 0.5g
Purified water balance (total amount is 100 g)

[Formulation Example 7] A lemon drink was prepared according to the following formulation.
50g sugar
Citric acid 1g
Lemon flavor 2g and fermented natto bacteria 5g
Purified water balance (total amount is 1000 g)

[Formulation Example 8] A low alcohol lemon drink was prepared according to the following formulation.
200g of commercial shellfish shochu (alcohol 20%)
50g sugar
Citric acid 1g
Lemon flavor 5g
Natto fermentation broth 5g
Purified water balance (total amount is 1000 g)

<Effect> A total of 10 men and women tested the cosmetics, tablets, and beverages of the above prescriptions for 3 months. As a result, many people gave results that the skin condition or body condition improved. Table 2
Panel ABCD EF GH I J
Cosmetic formulation example 1 ◎ ○ ○ △ ◎ ○ △ ○ ◎ ◎
Formulation Example 2 ○ ◎ ○ ◎ △ △ ◎ △ ○ △
Formulation Example 3 ◎ ○ ◎ △ ◎ ○ △ ○ ◎ ◎
Compounding Example 4 ○ ○ ○ ○ ○ ◎ ○ ◎ ○ ○
Formulation Example 5 ○ ◎ ○ ◎ ◎ ○ △ ○ ◎ ◎
Formulation Example 6 ◎ ○ △ △ ○ ◎ ◎ △ ○ ◎
Beverage / alcoholic beverage formulation example 7 △ ○ ○ △ ◎ ○ △ ○ ○ ○
Formulation Example 8 ○ ○ ○ ◎ ◎ ○ △ ○ ○ ○
note)
Evaluation display: ◎ considerably improved, ○ improved, △ not much changed, × worsened.
The display of evaluation is shown as an average result based on individual evaluation of 10 persons.

Claims (2)

  A biological function regulator having a function of removing active oxygen, comprising a fermented liquid obtained by mixing soybean extract, rush extract and krill extract and fermenting with natto. A cosmetic or food / beverage product having a function of removing active oxygen, comprising a fermented liquid obtained by mixing soybean extract, rush extract and krill extract and fermenting with natto bacteria.