CN110960481A - Preparation method of skin care composition for increasing exosome yield - Google Patents
Preparation method of skin care composition for increasing exosome yield Download PDFInfo
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- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
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- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
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- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
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- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
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- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
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- A61K8/4973—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom
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- A61K8/55—Phosphorus compounds
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- A61K8/00—Cosmetics or similar toiletry preparations
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- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
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- A61K8/00—Cosmetics or similar toiletry preparations
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- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
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- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
Abstract
The invention discloses a preparation method of a skin care product composition for increasing the yield of exosomes, and relates to the technical field of skin care products. Preparing raw materials: 50 parts of medlar cell culture extract, 0.15 part of surfactin sodium, 5 parts of glycerol, 44.85 parts of olive oil, 5 parts of squalane, 5 parts of caprylic/capric triglyceride, 6 parts of cetyl palmitate, 6 parts of sorbitan stearate, polysorbate-807 parts, 8 parts of hydrogenated lecithin, 109 parts of palmitoyl tetrapeptide-109 parts, 12 parts of water and 2 parts of essence. The addition of the medlar cell culture extract, the sodium surfactin and the palmitoyl tetrapeptide can effectively promote the production of collagen, further effectively reduce wrinkles, inhibit the generation of melanin and fade acne marks, so that the skin is smooth, inhibit the generation of melanin and fade acne marks, improve the skin, ensure the addition of glycerin and olive oil, ensure the good permeability of the skin, and further increase the metabolism of the skin and supplement nutrition.
Description
Technical Field
The invention relates to the technical field of skin care products, in particular to a preparation method of a skin care product composition for increasing the yield of exosomes.
Background
The skin has the effects of isolation and protection, and because of work fatigue and poor sleep, the skin aging symptoms such as tarnish, yellowing, poor skin elasticity and the like are caused, along with the acceleration of the life rhythm of people, the increase of work pressure and life pressure, the generation of environmental pollution and food problems, the skin health problems of people are more and more, and pigment disorder skin patients such as abnormal complexion, skin wrinkles, dry skin, greasy skin, color spots such as freckles, black spots, chloasma, senile plaques and the like are more and more common.
The existing exosome is widely applied to the aspect of skin care, the exosome is a small vesicle wrapped by a lipid molecular membrane, the exosome contains cytoplasm of source cells and lipid envelope components, the exosome contains rich mRNA, microRNA and protein components, the protein or RNA component contained in the exosome has the function of information communication with damaged cells, further the biological function of target cells can be regulated, various factors carried by the exosome can turn skin care to the cell level, the skin state is fundamentally youthful, and the skin is repaired.
Disclosure of Invention
Technical problem to be solved
In view of the deficiencies of the prior art, the present invention provides a method for preparing a skin care composition for increasing exosome production, which solves the problems set forth in the background art.
(II) technical scheme
In order to achieve the purpose, the invention provides the following technical scheme: a method of preparing a skin care composition for increasing exosome production comprising the steps of:
s1, preparing raw materials: 50 parts of medlar cell culture extract, 0.15 part of surfactin sodium, 5 parts of glycerol, 44.85 parts of olive oil, 5 parts of squalane, 5 parts of caprylic/capric triglyceride, 6 parts of cetyl palmitate, 6 parts of sorbitan stearate, polysorbate-807 parts, 8 parts of hydrogenated lecithin, 109 parts of palmitoyl tetrapeptide-109 parts, 12 parts of water and 2 parts of essence;
s2, weighing the raw materials for the first time: weighing the required surfactin sodium and glycerol according to the proportion, and putting the surfactin sodium and the glycerol into a beaker for later use;
s3, preparation of a first raw material: heating the raw materials in the beaker in the step S2, and stirring until the sodium surfactin and the glycerol in the beaker are completely dissolved and mixed for later use;
s4, adding raw materials: adding 5 parts of squalane into the raw material I in the beaker in the step S3, and stirring and homogenizing at a low speed to obtain the required raw material I for later use;
s5, weighing the raw materials for the second time: weighing the required water, caprylic/capric triglyceride, cetyl palmitate, sorbitan stearate, polysorbate-80, hydrogenated lecithin and palmitoyl tetrapeptide-10 according to the proportion, and filling in a measuring cup for later use;
s6, preparation of a second raw material: adding the raw materials in the measuring cup in the step S5 into the beaker in the step S4, stirring again, and uniformly mixing to obtain a required raw material II;
s7, weighing the raw materials for the third time, weighing the medlar cell culture extract, the olive oil and the essence according to the proportion, and filling the medlar cell culture extract, the olive oil and the essence into a measuring cup for later use;
s8, preparation of raw materials III: mixing the raw materials in the measuring cup in the step S7 and the raw material II obtained in the step S6, and uniformly stirring to obtain the required skin care product;
s9, standing, packaging and storing: and (5) standing the skin care product obtained in the step S8 for 10-12h, and packaging and storing the skin care product after standing.
As a specific optimization scheme of the embodiment, the low speed in the step S4 is low speed stirring at 200rpm-300rpm, and the homogenization time in the step S4 is 3-5 min.
As a specific optimization scheme of the embodiment, the stirring time in the step S6 is 5-8min, and the stirring time in the step S8 is 7-9 min.
As a specific optimization scheme of this embodiment, the essence comprises a skin conditioner and an acid-resistant preservative.
As a specific optimization scheme of the embodiment, the skin conditioner is dipotassium glycyrrhizinate, and the weight part of the skin conditioner in the essence is 0.7-1.2%; the acid-resistant preservative is phenoxyethanol, and the weight part of the acid-resistant preservative in the essence is 0.8-1.3%.
As a specific optimization scheme of the embodiment, the medlar cell culture extract is Phytocelltec GOJI of Mibelle.
(III) advantageous effects
Compared with the prior art, the invention provides a preparation method of the skin care product composition for increasing the yield of exosomes, which has the following beneficial effects:
1. the preparation method of the skin care product composition for increasing the yield of exosomes comprises the steps of adding the wolfberry cell culture extract, the sodium surfactin and the palmitoyl tetrapeptide, effectively promoting the production of collagen, further effectively reducing wrinkles, inhibiting melanin generation and fading acne marks, so that the skin is smooth, inhibiting melanin generation and fading acne marks, further improving the skin, adding the glycerin and the olive oil, ensuring good permeability and easy absorption of the skin, further ensuring a good moisturizing effect in the using process, further effectively entering the skin in the using process, further increasing the metabolism of the skin and supplementing nutrition.
2. The skin care product provided by the invention has the advantages that the components are cooperatively matched, the obtained skin care product has stable performance, the nutrient components are matched, the effect of promoting the yield of exosomes in the skin care product is achieved, the fibroblast is proliferated, the increase of collagen and elastin is ensured, the appearance of the skin is improved, the skin tightening effect is achieved, the wrinkle depth is reduced, and the skin elasticity is improved.
(IV) description of the drawings
FIG. 1 is a schematic flow chart of a method for producing a skin care composition with increased exosome production according to the present invention;
FIG. 2 is a schematic diagram showing the comparison of test results of experimental examples of a method for preparing a skin care composition for increasing the production of exosomes according to the present invention;
FIG. 3 is a schematic diagram showing two quantitative methods in an experimental example of a method for preparing a skin care composition for increasing exosome production according to the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The first embodiment is as follows: referring to fig. 1, the present invention discloses a method for preparing a skin care composition for increasing exosome production, comprising the steps of:
s1, preparing raw materials: 50 parts of medlar cell culture extract, 0.15 part of surfactin sodium, 5 parts of glycerol, 44.85 parts of olive oil, 5 parts of squalane, 5 parts of caprylic/capric triglyceride, 6 parts of cetyl palmitate, 6 parts of sorbitan stearate, polysorbate-807 parts, 8 parts of hydrogenated lecithin, 109 parts of palmitoyl tetrapeptide-109 parts, 12 parts of water and 2 parts of essence;
s2, weighing the raw materials for the first time: weighing the required surfactin sodium and glycerol according to the proportion, and putting the surfactin sodium and the glycerol into a beaker for later use;
s3, preparation of a first raw material: heating the raw materials in the beaker in the step S2, and stirring until the sodium surfactin and the glycerol in the beaker are completely dissolved and mixed for later use;
s4, adding raw materials: adding 5 parts of squalane into the raw material I in the beaker in the step S3, and stirring and homogenizing at a low speed to obtain the required raw material I for later use;
s5, weighing the raw materials for the second time: weighing the required water, caprylic/capric triglyceride, cetyl palmitate, sorbitan stearate, polysorbate-80, hydrogenated lecithin and palmitoyl tetrapeptide-10 according to the proportion, and filling in a measuring cup for later use;
s6, preparation of a second raw material: adding the raw materials in the measuring cup in the step S5 into the beaker in the step S4, stirring again, and uniformly mixing to obtain a required raw material II;
s7, weighing the raw materials for the third time, weighing the medlar cell culture extract, the olive oil and the essence according to the proportion, and filling the medlar cell culture extract, the olive oil and the essence into a measuring cup for later use;
s8, preparation of raw materials III: mixing the raw materials in the measuring cup in the step S7 and the raw material II obtained in the step S6, and uniformly stirring to obtain the required skin care product;
s9, standing, packaging and storing: and (5) standing the skin care product obtained in the step S8 for 10h, and packaging and storing the skin care product after standing.
As a specific optimization scheme of the embodiment, the low speed in the step S4 is low speed stirring at 200rpm-300rpm, and the homogenization time in the step S4 is 3 min.
As a specific optimization scheme of this embodiment, the stirring time in step S6 is 5min, and the stirring time in step S8 is 7 min.
As a specific optimization scheme of this embodiment, the essence comprises a skin conditioner and an acid-resistant preservative.
As a specific optimization scheme of the embodiment, the skin conditioner is dipotassium glycyrrhizinate, and the weight part of the skin conditioner in the essence is 0.7%; the anti-acid preservative is phenoxyethanol, and the weight part of the anti-acid preservative in the essence is 1.3%.
As a specific optimization scheme of the embodiment, the medlar cell culture extract is Phytocelltec GOJI of Mibelle.
Example two: referring to fig. 1, the present invention discloses a method for preparing a skin care composition for increasing exosome production, comprising the steps of:
s1, preparing raw materials: 50 parts of medlar cell culture extract, 0.15 part of surfactin sodium, 5 parts of glycerol, 44.85 parts of olive oil, 5 parts of squalane, 5 parts of caprylic/capric triglyceride, 6 parts of cetyl palmitate, 6 parts of sorbitan stearate, polysorbate-807 parts, 8 parts of hydrogenated lecithin, 109 parts of palmitoyl tetrapeptide-109 parts, 12 parts of water and 2 parts of essence;
s2, weighing the raw materials for the first time: weighing the required surfactin sodium and glycerol according to the proportion, and putting the surfactin sodium and the glycerol into a beaker for later use;
s3, preparation of a first raw material: heating the raw materials in the beaker in the step S2, and stirring until the sodium surfactin and the glycerol in the beaker are completely dissolved and mixed for later use;
s4, adding raw materials: adding 5 parts of squalane into the raw material I in the beaker in the step S3, and stirring and homogenizing at a low speed to obtain the required raw material I for later use;
s5, weighing the raw materials for the second time: weighing the required water, caprylic/capric triglyceride, cetyl palmitate, sorbitan stearate, polysorbate-80, hydrogenated lecithin and palmitoyl tetrapeptide-10 according to the proportion, and filling in a measuring cup for later use;
s6, preparation of a second raw material: adding the raw materials in the measuring cup in the step S5 into the beaker in the step S4, stirring again, and uniformly mixing to obtain a required raw material II;
s7, weighing the raw materials for the third time, weighing the medlar cell culture extract, the olive oil and the essence according to the proportion, and filling the medlar cell culture extract, the olive oil and the essence into a measuring cup for later use;
s8, preparation of raw materials III: mixing the raw materials in the measuring cup in the step S7 and the raw material II obtained in the step S6, and uniformly stirring to obtain the required skin care product;
s9, standing, packaging and storing: and (5) standing the skin care product obtained in the step (S8) for 11h, and packaging and storing the skin care product after standing.
As a specific optimization scheme of the embodiment, the low speed in the step S4 is low speed stirring at 200rpm-300rpm, and the homogenization time in the step S4 is 4 min.
As a specific optimization scheme of this embodiment, the stirring time in step S6 is 6min, and the stirring time in step S8 is 8 min.
As a specific optimization scheme of this embodiment, the essence comprises a skin conditioner and an acid-resistant preservative.
As a specific optimization scheme of the embodiment, the skin conditioner is dipotassium glycyrrhizinate, and the weight part of the skin conditioner in the essence is 1.1%; the anti-acid preservative is phenoxyethanol, and the weight part of the anti-acid preservative in the essence is 0.9%.
As a specific optimization scheme of the embodiment, the medlar cell culture extract is Phytocelltec GOJI of Mibelle.
Example three: referring to fig. 1, the present invention discloses a method for preparing a skin care composition for increasing exosome production, comprising the steps of:
s1, preparing raw materials: 50 parts of medlar cell culture extract, 0.15 part of surfactin sodium, 5 parts of glycerol, 44.85 parts of olive oil, 5 parts of squalane, 5 parts of caprylic/capric triglyceride, 6 parts of cetyl palmitate, 6 parts of sorbitan stearate, polysorbate-807 parts, 8 parts of hydrogenated lecithin, 109 parts of palmitoyl tetrapeptide-109 parts, 12 parts of water and 2 parts of essence;
s2, weighing the raw materials for the first time: weighing the required surfactin sodium and glycerol according to the proportion, and putting the surfactin sodium and the glycerol into a beaker for later use;
s3, preparation of a first raw material: heating the raw materials in the beaker in the step S2, and stirring until the sodium surfactin and the glycerol in the beaker are completely dissolved and mixed for later use;
s4, adding raw materials: adding 5 parts of squalane into the raw material I in the beaker in the step S3, and stirring and homogenizing at a low speed to obtain the required raw material I for later use;
s5, weighing the raw materials for the second time: weighing the required water, caprylic/capric triglyceride, cetyl palmitate, sorbitan stearate, polysorbate-80, hydrogenated lecithin and palmitoyl tetrapeptide-10 according to the proportion, and filling in a measuring cup for later use;
s6, preparation of a second raw material: adding the raw materials in the measuring cup in the step S5 into the beaker in the step S4, stirring again, and uniformly mixing to obtain a required raw material II;
s7, weighing the raw materials for the third time, weighing the medlar cell culture extract, the olive oil and the essence according to the proportion, and filling the medlar cell culture extract, the olive oil and the essence into a measuring cup for later use;
s8, preparation of raw materials III: mixing the raw materials in the measuring cup in the step S7 and the raw material II obtained in the step S6, and uniformly stirring to obtain the required skin care product;
s9, standing, packaging and storing: and (5) standing the skin care product obtained in the step S8 for 12h, and packaging and storing the skin care product after standing.
As a specific optimization scheme of the embodiment, the low speed in the step S4 is low speed stirring at 200rpm-300rpm, and the homogenization time in the step S4 is 5 min.
As a specific optimization scheme of this embodiment, the stirring time in step S6 is 8min, and the stirring time in step S8 is 9 min.
As a specific optimization scheme of this embodiment, the essence comprises a skin conditioner and an acid-resistant preservative.
As a specific optimization scheme of the embodiment, the skin conditioner is dipotassium glycyrrhizinate, and the weight part of the skin conditioner in the essence is 1.2%; the anti-acid preservative is phenoxyethanol, and the weight part of the anti-acid preservative in the essence is 0.8%.
As a specific optimization scheme of the embodiment, the medlar cell culture extract is Phytocelltec GOJI of Mibelle.
Experimental example:
as shown in FIG. 2, the test results show that the wrinkle can be effectively reduced and the skin can be smoothened
As shown in fig. 3, two quantitative methods showed that the culture extract of lycium barbarum cells was effective in increasing exosome production.
And (4) judging the standard: through comparison of the three embodiments, the best effect is the second embodiment, so that the second embodiment is selected as the best embodiment, and the specific change of the amount also belongs to the protection scope of the technical scheme.
The invention has the beneficial effects that: the addition of the medlar cell culture extract, the sodium surfactin and the palmitoyl tetrapeptide can effectively promote the production of collagen, further effectively reduce wrinkles, inhibit the generation of melanin and fade acne marks, so that the skin is smooth, inhibit the generation of melanin and fade acne marks, improve the skin, improve the addition of glycerin and olive oil, ensure the good permeability and easy absorption effect of the skin, ensure the good moisturizing effect in the using process, and effectively enter the skin in the using process, thereby increasing the metabolism of the skin and supplementing nutrition; the skin care product provided by the invention has the advantages that the squalane, caprylic/capric triglyceride, cetyl palmitate, sorbitan stearate, polysorbate-80 and hydrogenated lecithin are added in a reasonable proportion, so that the skin care product contains various antioxidant active ingredients, the metabolism of cells is further promoted, and the repair of damaged cells is facilitated.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. A method of preparing a skin care composition for increasing exosome production comprising the steps of:
s1, preparing raw materials: 50 parts of medlar cell culture extract, 0.15 part of surfactin sodium, 5 parts of glycerol, 44.85 parts of olive oil, 5 parts of squalane, 5 parts of caprylic/capric triglyceride, 6 parts of cetyl palmitate, 6 parts of sorbitan stearate, polysorbate-807 parts, 8 parts of hydrogenated lecithin, 109 parts of palmitoyl tetrapeptide-109 parts, 12 parts of water and 2 parts of essence;
s2, weighing the raw materials for the first time: weighing the required surfactin sodium and glycerol according to the proportion, and putting the surfactin sodium and the glycerol into a beaker for later use;
s3, preparation of a first raw material: heating the raw materials in the beaker in the step S2, and stirring until the sodium surfactin and the glycerol in the beaker are completely dissolved and mixed for later use;
s4, adding raw materials: adding 5 parts of squalane into the raw material I in the beaker in the step S3, and stirring and homogenizing at a low speed to obtain the required raw material I for later use;
s5, weighing the raw materials for the second time: weighing the required water, caprylic/capric triglyceride, cetyl palmitate, sorbitan stearate, polysorbate-80, hydrogenated lecithin and palmitoyl tetrapeptide-10 according to the proportion, and filling in a measuring cup for later use;
s6, preparation of a second raw material: adding the raw materials in the measuring cup in the step S5 into the beaker in the step S4, stirring again, and uniformly mixing to obtain a required raw material II;
s7, weighing the raw materials for the third time, weighing the medlar cell culture extract, the olive oil and the essence according to the proportion, and filling the medlar cell culture extract, the olive oil and the essence into a measuring cup for later use;
s8, preparation of raw materials III: mixing the raw materials in the measuring cup in the step S7 and the raw material II obtained in the step S6, and uniformly stirring to obtain the required skin care product;
s9, standing, packaging and storing: and (5) standing the skin care product obtained in the step S8 for 10-12h, and packaging and storing the skin care product after standing.
2. The method for preparing a skin care composition for increasing exosome production according to claim 1, wherein the low speed in the step S4 is to stir uniformly at a low speed of 200rpm to 300rpm, and the homogenization time in the step S4 is 3 to 5 min.
3. The method for preparing a skin care composition for increasing exosome production according to claim 1, wherein the stirring time in step S6 is 5-8min, and the stirring time in step S8 is 7-9 min.
4. The method of preparing a skin care composition for increasing exosome production according to claim 1, wherein the serum comprises a skin conditioner and an acid-resistant preservative.
5. The method for preparing a skin care composition for increasing the yield of exosomes according to claim 4, wherein the skin conditioner is dipotassium glycyrrhizinate, and the weight portion of the skin conditioner in the essence is 0.7-1.2%; the acid-resistant preservative is phenoxyethanol, and the weight part of the acid-resistant preservative in the essence is 0.8-1.3%.
6. The method of claim 1, wherein the culture extract of Lycium barbarum is Phytocelltec GOJI from Mibelle.
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111773155A (en) * | 2020-07-10 | 2020-10-16 | 陕西慧康生物科技有限责任公司 | Stable peptide containing active matter from fermentation source |
CN113425620A (en) * | 2021-06-17 | 2021-09-24 | 华熙生物科技股份有限公司 | Liposome for wrapping active component, preparation method and application thereof |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104523522A (en) * | 2014-12-23 | 2015-04-22 | 吴辰 | Lycium ruthenicum fermented facial mask |
CN107982200A (en) * | 2017-12-05 | 2018-05-04 | 广州赛莱拉干细胞科技股份有限公司 | A kind of anti-wrinkle skin care item containing Chinese wolfberry fruit dry cell extract and preparation method thereof |
CN108004328A (en) * | 2017-12-12 | 2018-05-08 | 杜立波 | A kind of Skin whitening care cosmetics method for customizing |
CN109200008A (en) * | 2018-08-29 | 2019-01-15 | 华妍堂(北京)生物科技有限责任公司 | A kind of eye cream and preparation method thereof |
CN109394639A (en) * | 2018-11-12 | 2019-03-01 | 广东科玮生物技术股份有限公司 | A kind of anti-ageing activating essence and preparation method thereof containing ferment |
CN110037948A (en) * | 2019-05-28 | 2019-07-23 | 儒兰(福建)生物科技有限公司 | A kind of tax eye cream living and preparation method thereof |
-
2019
- 2019-12-20 CN CN201911330467.1A patent/CN110960481A/en active Pending
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104523522A (en) * | 2014-12-23 | 2015-04-22 | 吴辰 | Lycium ruthenicum fermented facial mask |
CN107982200A (en) * | 2017-12-05 | 2018-05-04 | 广州赛莱拉干细胞科技股份有限公司 | A kind of anti-wrinkle skin care item containing Chinese wolfberry fruit dry cell extract and preparation method thereof |
CN108004328A (en) * | 2017-12-12 | 2018-05-08 | 杜立波 | A kind of Skin whitening care cosmetics method for customizing |
CN109200008A (en) * | 2018-08-29 | 2019-01-15 | 华妍堂(北京)生物科技有限责任公司 | A kind of eye cream and preparation method thereof |
CN109394639A (en) * | 2018-11-12 | 2019-03-01 | 广东科玮生物技术股份有限公司 | A kind of anti-ageing activating essence and preparation method thereof containing ferment |
CN110037948A (en) * | 2019-05-28 | 2019-07-23 | 儒兰(福建)生物科技有限公司 | A kind of tax eye cream living and preparation method thereof |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111773155A (en) * | 2020-07-10 | 2020-10-16 | 陕西慧康生物科技有限责任公司 | Stable peptide containing active matter from fermentation source |
CN111773155B (en) * | 2020-07-10 | 2022-11-04 | 陕西慧康生物科技有限责任公司 | Active matter stabilizing peptide containing fermentation source |
CN113425620A (en) * | 2021-06-17 | 2021-09-24 | 华熙生物科技股份有限公司 | Liposome for wrapping active component, preparation method and application thereof |
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