CN108004328A

CN108004328A - A kind of Skin whitening care cosmetics method for customizing

Info

Publication number: CN108004328A
Application number: CN201711316539.8A
Authority: CN
Inventors: 杜立波
Original assignee: Individual
Current assignee: Individual
Priority date: 2017-12-12
Filing date: 2017-12-12
Publication date: 2018-05-08

Abstract

The invention discloses a kind of Skin whitening care cosmetics method for customizing, comprise the steps of：Step 1, user's cell sample is gathered；Step 2, the mRNA in cell sample is extracted；Step 3, mRNA reverse transcriptions are synthesized into cDNA；Step 4, the expression quantity of the polymorphism of colour of skin related gene and corresponding cDNA in cDNA are detected, colour of skin related gene includes at least one of MC1R, GSTP1, SLC24A5；Step 5, judge whitening capability according to gene pleiomorphism and corresponding cDNA expression quantity, and for whitening capability selection Skin whitening care cosmetics component and dosage, prepare suitable Skin whitening care cosmetics.This method obtains the whitening capability of different user by measuring the expression quantity of colour of skin related gene, and prepares suitable Skin whitening care cosmetics according to the specific whitening capability of user.

Description

A kind of Skin whitening care cosmetics method for customizing

Technical field

The present invention relates to a kind of technical field of skin care, more particularly to a kind of Skin whitening care cosmetics method for customizing.

Background technology

With the development of science and technology, the full genome spectrogram of the mankind can be obtained by being sequenced.Currently largely grind Study carefully and show, state and the gene of human skin have close relationship.The genotype of specific site in these colour of skin related genes (or gene mononucleotide polymorphism, SNPs, abbreviation gene pleiomorphism) can largely affect the state of skin, different Genotype be to cause the key factor of skin problems.People can have found or predict skin by detecting different genotype The problem of skin, and select suitable Skin whitening care cosmetics in this, as guidance.Further study show that in addition to genotype, by gene Expressing protein caused by type is only the key factor for causing skin problem.

It currently there are a large amount of skin cares with difference in functionality and beauty product selected for people.But per each and every one The skin of body all has its uniqueness, for example, the whitening of different application on human skin is horizontal and whitening capability differs.How to select It is exactly the major issue that people face to be adapted to the Skin whitening care cosmetics of oneself.The method that current consumer generally selects is exactly to adopt Skin whitening care cosmetics are verified if appropriate for oneself with the mode of selection-purchase-trial, and consumer not only wastes greatly in the process The time of amount and financial resources, but also it is likely to result in the discomfort of skin.Therefore the whitening shield for being adapted to consumer's individual how is selected Skin product, become a major issue urgently to be resolved hurrily.

The content of the invention

The present invention provides the skin care item method for customizing based on genetic test, and this method is by measuring the more of skin-related gene The expression quantity of state property and corresponding cDNA obtain the skin condition of different user, and prepare what is be adapted to according to the specific skin condition of user Skin care item.

According to a kind of Skin whitening care cosmetics method for customizing of the present invention, comprise the steps of：

Step 1, user's cell sample is gathered；

Step 2, the mRNA in cell sample is extracted；

Step 3, mRNA reverse transcriptions are synthesized into cDNA；

Step 4, the expression quantity of the polymorphism of colour of skin related gene and corresponding cDNA in cDNA are detected, wherein, the colour of skin is related Gene includes at least one of MC1R, GSTP1, SLC24A5；

Step 5, the expression quantity according to gene pleiomorphism and corresponding cDNA judges whitening capability, and is selected for whitening capability Component and dosage, prepare suitable Skin whitening care cosmetics.

Further, step 4 includes：

Step 4a), the mRNA sequence of colour of skin related gene is searched on NCBI, and design corresponding primer；

Step 4b), using delta-deltaCt methods, using gene GAPDH as internal reference, pass through primer detection colour of skin dependency basis The relative expression quantity of the polymorphism of cause and corresponding cDNA.

Further, primer includes：

GAPDH Forword：5’-CGGGAAACTGTGGCGTGATG-3’

Reverse：5’-ATGACCTTGCCCACAGCCTT-3’

MC1R Forword：5’-CACTGCGCTACCACAGCATC-3’

Reverse：5’-GCGTGCTGAAGACGACACTG-3’

GSTP1 Forword：5’-GCCAGAGCTGGAAGGAGGAG-3’

Reverse：5’-GCAGGAGGCTTTGAGTGAGC-3’

SLC24A5 Forword：5’-CTGTGGGCCACTGCACATC-3’

Reverse：5’-TGGCCCTTGGGAGACGTTG-3’.

Further, the component of Skin whitening care cosmetics includes skin conditioning agent, polypeptide and auxiliary agent.

Further, skin conditioning agent includes Gotu Kola P.E, lycopene, ceramide, lecithin, propolis extraction Thing, chamomile extract, ginseng root extract, yeast extract, the one or more of houttuynia extract.

Further, polypeptide include alanine/histidine/lysine polypeptide copper HCl, soybean (GLYCINEMAX) polypeptide, Dipeptides -1, dipeptides -2, dipeptides -4, oligopeptides -1, oligopeptides -2, oligopeptides -3, oligopeptides -4, oligopeptides -5, oligopeptides -6, oligopeptides -29, oligopeptides - 34th, carnosine, saccharomycete polypeptide, arginine/lysine polypeptide, nonapeptide -1, poly- (tripeptides -6), ascorbyl polypeptide, withered grass bacterium Lipopeptid sodium, hexapeptide -1,2,3,5,9,11, heptapeptide -6, myristoyl hexapeptide -5, myristoyl pentapeptide -4, tripeptides -1,2,3,10, 32nd, the copper of tripeptides -1, decapeptide -4, tetrapeptide -1,3,4, pentapeptide -1,3,34, oat peptide, Wild soybean peptide, acetyl group octapeptide -3, acetyl group The cetyl of dipeptides -1, acetyl group hexapeptide 1,7,8, acetyl group heptapeptide -4, acetyl group tetrapeptide 2,3,5,9,11, palmityl dipeptides -7, Palmityl hexapeptide -12,14,15, palmityl tripeptides -1,5,8, palmityl tetrapeptide -5,7,10, one kind in Matrixyl -4,5 Or several combination.

Further, auxiliary agent includes first group of moisturizer, second group of moisturizer, grease, solvent, preservative, solubilizer, Thickener, skin conditioning agent, chelating agent, aromatic, anti-sensitizer and water.

Further, step 5 includes：

Step 5a) for first group of moisturizer of user's skin condition selection appropriate mass ratio of skin care item, second group of moisturizing Agent, grease, organic solvent, preservative, solubilizer, thickener, skin conditioning agent, chelating agent, antioxidant, polypeptide, fragrance Agent, anti-sensitizer and water；

Step 5b) solubilizer, antioxidant, grease, organic solvent and aromatic exceed the speed limit in mulser to be stirred, The mixture A of system；

Step 5c) first group of moisturizer be put into stirring and dissolving in agitated kettle, the mixture B of system；

Step 5d) second group of moisturizer, chelating agent, thickener are added in agitated kettle, warming while stirring to 85-95 DEG C, it is fully dissolved, keep the temperature 15-20 minutes, the mixture C of system；

Step 5e) mixture C is cooled to 40-45 DEG C, successively plus skin conditioning agent, anti-sensitizer and polypeptide, stirring and dissolving, The mixture D of system；

Step 5f) in the case where keeping 40-45 DEG C of temperature, add preservative into mixture D, mixture A, mixture B, fully Stirring, is down to room temperature, discharging, stand.

Further, the component of Skin whitening care cosmetics and mass ratio are：First group of moisturizer 2-7 parts by weight, second group of moisturizing Agent 2-11 parts by weight, grease 2-11 parts by weight, organic solvent 4-6 parts by weight, preservative 0.2-4 parts by weight, solubilizer 4-6 weight Part, thickener 2-3 parts by weight, skin conditioning agent 2-10 parts by weight, chelating agent 0.1-0.4 parts by weight, antioxidant 1-5 weight Part, polypeptide 0.5-2.5 parts by weight, aromatic 0.02-0.03 parts by weight, anti-sensitizer 0.1-1.5 parts by weight, water 40-70 weight Part.

Further, the component of Skin whitening care cosmetics and mass ratio are：First group of 3 parts by weight of moisturizer, second group of moisturizing 5 parts by weight of agent, 7.6 parts by weight of grease, 4 parts by weight of organic solvent, 0.2 parts by weight of preservative, 4 parts by weight of solubilizer, thickener 2 Parts by weight, 4 parts by weight of skin conditioning agent, 0.2 parts by weight of chelating agent, 2.4 parts by weight of antioxidant, 0.6 parts by weight of polypeptide, virtue 0.02 parts by weight of pastil, 0.1 parts by weight of anti-sensitizer, 67 parts by weight of water.

Due to being used in upper technical solution, the present invention has the following advantages that compared with prior art：

(1) method for customizing according to the present invention realizes that a people one is formulated, and is prepared for the specific whitening capability of different user Skin whitening care cosmetics with personal exclusive formula.

(2) expression quantity of colour of skin related gene polymorphism and its corresponding cDNA, which are used as, causes skin that different whitening shapes are presented The basic reason of state can more accurately characterize whitening capability, of the invention according to colour of skin related gene polymorphism and its corresponding The expression quantity of cDNA judges whitening capability, compared with the prior art in graphical analysis, the detection method such as facial scan obtains Testing result it is more accurate.

(3) method for customizing according to the present invention selects Oral Mucosal Cells or skin of face cast-off cells as pattern detection The expression quantity of colour of skin related gene polymorphism and its corresponding cDNA, it is ensured that skin is from laser or the drug induced external world Stimulate, securely and reliably.

(4) according to the present invention method for customizing prepare Skin whitening care cosmetics use more natural animal-plant extract and polypeptide for Raw material, it is gently non-stimulated to skin, and Skin whitening care cosmetics can have effects that other skin care item are used cooperatively, skin effect It is good.

(5) skin care item prepared according to the method for customizing of the present invention are directed to improving the quick ability of whitening of user itself, phase Than for the existing skin care item such as exterior moisturizing, hyperchromic are relied on, can fundamentally solve the skin problem of user.

(6) present invention realizes new opplication of the genes such as MC1R, GSTP1, SLC24A5 in skin care field.

Brief description of the drawings

In specific embodiment shown in the drawings, its purpose is used to illustrate the present invention.It should be appreciated, however, that the present invention is simultaneously It is not limited to shown preferred embodiment.

In schema：

Fig. 1 is the schematic diagram according to one embodiment of Skin whitening care cosmetics method for customizing of the present invention.

Embodiment

Unless otherwise defined, all meanings having in as used herein technical and scientific terms, for belonging to Technical staff is general knowledge in the technical field of the present invention.When being used herein as, following term has the meaning belonging to it, unless Separately there is special definition.

Gene expression (geneexpression) " refers to cell in life process, believes heredity in DNA sequence is stored in Breath passes through transcription and translation, is transformed into biologically active protein molecule.Same gene can generate difference in different tissues Gene outcome derive from the albuminoids of different tissues, can be encoded and produced by same gene, this phenomenon be firstly because Enhancer in gene etc. is specific in a organized way, it can be combined with the organizing specific factor in different tissues, therefore in different tissues Middle same gene can produce different transcripts and transcription post-processing effect.

" gene pleiomorphism " refers to some specific genetic locus (gene order or non-base in a colony with appropriate frequency Because of sequence) there is a phenomenon where two or more variation, it can be determined by Direct Analysis DNA or gene outcome.From essence On say, the generation of polymorphism is the variation on gene level, typically occur in gene order the not region of encoding proteins and There is no the region of important regulatory function.

" polymerase chain reaction (reversetranscription-polymerasechainreaction, RT-PCR) " is The technology that the polymerase chain amplification (PCR) of the reverse transcription (reversetranscription) of RNA and cDNA is combined.It is first The first effect through reverse transcriptase synthesizes cDNA from RNA, then using cDNA as template, amplification synthesis purpose fragment.RT-PCR technology spirit Quick and widely used, available for gene expression dose in detection cell, the content of RNA virus and Direct Cloning are specific in cell The cDNA sequence of gene.RNA as template can be the RNA products of total serum IgE, mRNA or in-vitro transcription.No matter which kind of is used RNA, it is important to ensure the pollution without RNase and genomic DNA in RNA.This method utilizes correlation technique extraction tissue or cell In total serum IgE, using mRNA therein as template, using Oligo (dT) or random primer using reverse transcriptase reverse transcription into cDNA.It is again template through row PCR amplification using cDNA, and obtains target gene or detection gene expression.Real-time PCR (real- TimePCR), belong to one kind of quantitative PCR (Q-PCR), quantifying for DNA is carried out based on the amount of amplification of DNA in certain time Analysis.

As shown in Figure 1, the skin care item method for customizing based on genetic test according to the present invention mainly comprises the steps of：

Step 1, user's cell sample is gathered.The cell sample can make Buccal mucosa cell, skin of face come off Cell or other can extract the cell of user's gene.

In an embodiment of the present invention, the specific steps for gathering Buccal mucosa cell include preparation before (1) collection：Clearly Water is gargled, it should be noted that in order to ensure that cell sample is not contaminated, gathers the half an hour before Oral Mucosal Cells, to the greatest extent Amount not drink the drink containing caffeine, the beverage containing carbonic acid or fruit juice etc.；(2) Buccal mucosa cell is gathered： Buccal swab packaging is opened, swallowing act is first done, buccal swab is put in into oral cavity afterwards, examination 20 is scraped back and forth on the inside of cheek It is secondary, buccal swab is taken out, and mark is carried out, genomic DNA is extracted immediately or is put in added with 0.5ml buccal swab genes Group DNA preservation solution (win, article No. by hundred Aurions：BTN80802 preserved in 1.5ml centrifuge tubes).

In another embodiment of the invention, the specific steps for gathering facial skin exfoliation cell include standard before (1) collection It is standby：Clear water washes one's face, it should be noted that in order to ensure that cell sample is not contaminated, testee sheds all make with makeup remover Cosmetics remain；(2) collection face comes off Skin Cell：After waiting face dry, with cell Adhesive-fetch device in face repeatedly The viscous Skin Cell (if the hypocellularity of collection, several Adhesive-fetch devices collection cells can be used) for taking face.Glue after taking Properly preserve, avoid polluting.Pay attention to carrying out mark, prevent from obscuring sample.

Step 2, the mRNA in cell sample is extracted.The embodiment of the present invention is using QIAGEN companies RNeasyPlusMicroKit (article No.s：74034) Total RNAs extraction is carried out, is comprised the following steps that：

A) Buccal mucosa cell sample is directed to, buccal swab is put into the 1.5ml centrifuge tubes of RNase-free, is cut Buccal swab shank is removed, stays absorbent cotton head in centrifuge tube；For skin of face cast-off cells sample, with tweezers by cell Adhesive-fetch device On be stained with the glued membrane of Skin Cell and torn, be put into the 1.5ml centrifuge tubes of RNase-free and (pay attention to：Each sample uses one A tweezers, avoid the pollution between sample).The RLT buffer solution cell lysis of 350 μ l is added, vortex 1min is mixed.

B) cell pyrolysis liquid in previous step is added in the centrifugal column for the removal gDNA that kit provides,>10,000rpm Centrifuge 30s.Centrifugal column is lost, retains filtered solution.

C) 70% ethanol that isometric (being usually 350 μ l) is added in filtered solution mixes.

D) sample (precipitation for including formation) of previous step is transferred to the RNeasyMinElute that is provided in kit from In stem,>10,000rpm centrifugation 15s, abandon filtered solution.

E) 700 μ l buffer solution RW1 are added into RNeasyMinElute centrifugal columns,>10,000rpm centrifugation 15s, abandon filtration Liquid.

F) 500 μ l buffer solution RPE are added into RNeasyMinElute centrifugal columns,>10,000rpm centrifugation 15s, abandon filtration Liquid.

G) 500 μ l80% ethanol are added into RNeasyMinElute centrifugal columns,>10,000rpm centrifugation 2min, abandon filtration Liquid.

H) RNeasyMinElute centrifugal columns are transferred in the new 2ml collecting pipes that kit provides, maximum turn of uncapping Speed centrifugation 5min.Abandon collecting pipe and filtered solution therein.

I) RNeasyMinElute centrifugal columns are transferred in the new 1.5ml centrifuge tubes that kit provides, to centrifugal column Center adds the RNase-freewater of 14 μ l, and after standing 2min, maximum speed centrifugation 1min, interior centrifuge tube is extraction RNA。

J) concentration and quality (A260/A280) of NanoDrop (Thermo) spectrophotometric determinations RNA is used, is taken immediately 1 μ gRNA carry out reverse transcription or by the RNA of extraction in -80 DEG C of preservations.

Step 3, mRNA reverse transcriptions are synthesized into cDNA.The embodiment of the present invention is using TOYOBO companies ReverTraAceqPCRRTMasterMixwithgDNARemover (article No.s：FSQ-301 the synthesis of cDNA) is carried out.Specific behaviour It is as follows to make step：

A) mixing of 4xDNMasterMix and gDNARemover：The addition in homogeneous tube 4xDNMasterMix (440 μ l) The gDNARemover of 8.8 μ l (1/50 amount), overturns and mixes.The 4xDNMasterMix after gDNARemover is added in -20 DEG C of bars The stabilization of 3 months can be at least kept under part.

B) denaturation of RNA：1 μ gRNA, thermal denaturation after five minutes, is immediately placed on cooled on ice under the conditions of 65 DEG C.

C) genomic DNA reaction (DNase reactions) is removed：Wherein, reaction solution is formulated as follows on ice.

After reaction solution is lightly stirred evenly, incubated 5 minutes under the conditions of 37 DEG C.

D) reverse transcription reaction：Wherein, reaction solution is formulated as follows on ice.

After reaction solution is lightly stirred evenly, reacted by temperature below.

After reaction, preserve under the conditions of -20 DEG C.During Real-timeRTPCR, added after diluting 10 times as template.

Step 4, the expression quantity of the polymorphism of skin-related gene and corresponding cDNA in cDNA are detected.

In an embodiment of the present invention, skin-related gene can include one in the genes such as MC1R, GSTP1, SLC24A5 Kind or several combinations.

MC1R (melanocortin receptor expressing gene) is colour of skin related gene, it is located at No. 16 Chromosome 16q 24.3 Position, full length gene 8.86kb.The mRNA total length 3099nt of MC1R, encode the albumen being made of 317 amino acid residues.Gene Function：MC1R genes are also referred to as melanocyte-stimulating hormone acceptor gene, are encoded by spreading site, are control skin black The important gene of element synthesis, i.e. the generative capacity of the human melanin of the genotype is high, and the gene expression amount is higher, uvioresistant The risk of line allergy is low, the easier blackening of skin.

GSTP1 (Glutathione S-transferases M1) is colour of skin related gene, it is located at o.11 Chromosome 11q13 .2 Put, full length gene 3.07kb, share 9 extrons.The mRNA total length 965nt of GSTP1, encode by 210 amino acid residue groups Into albumen.Gene function：GSTP1 can make to have on the cysteine residues of glutathione very the mercapto groups of strong affinity with Electrophilic agent combines, and adjusts that skin is endogenous and the metabolism of exogenous compounds, maintains the intrinsic stability of skin.GSTP1 with Skin pigment it is calm closely related, i.e. people's pigmentation ability of the genotype is high, and the gene expression amount is higher, anti-purple The risk of outside line allergy is low, the easier blackening of skin.

SLC24A5 (24 member 5 of sapiens's Solute Carrier family) is colour of skin related gene, it is located at No. 15 chromosome 15q21.1 Position, full length gene 21.7kb, shares 7 extrons.The mRNA total length 1617nt of SLC24A5, encode residual by 500 amino acid The albumen of base composition.Gene function：A kind of potassium ion on melanosome (melanosome) film of SLC24A5 gene codes Dependence cationic exchange protein, is responsible for calcium ion taking in melanosome, and sodium ion is pumped out out of this organelle, the latter Join with the effect phase lotus root of proton pump, this has important work to the hydrogen ion inside and outside maintenance melanosome and Calcium gradient With, the formation of this ion gradient participates in building-up process of the melanin in melanosome, therefore has substantial connection with the colour of skin, That is, the generative capacity of the human melanin of the genotype is high, and the gene expression amount is higher, and the risk of uvioresistant allergy is low, skin The easier blackening of skin.

The embodiment of the present invention is comprised the following steps that using the amount of real-timeRTPCR detection related genes：

A) mRNA sequence of related gene is searched on NCBI, and designs corresponding primer.

The embodiment of the present invention is using anxious gene GAPDH as internal reference, i.e. sets GAPDH gene expression amounts as 1, uses Delta-deltaCt methods carry out relative quantitative assay to above-mentioned skin-related gene.The primer sequence of GAPDH and skin-related gene Row are as shown in table 1：

Table 1

The amplified fragments sequence of each gene shown in table 1 is as shown in table 2：

Table 2

B) using the SYBRGreenRealtimePCRMasterMix (article No.s of TOYOBO companies：QPK-201) carry out Detection, using Roche companiesMachine testing on 96.

The preparation program of reaction solution is as shown in table 3：

Table 3

The cycling condition of PCR is as shown in table 4：

Table 4

C) data analysis：Solubility curve is analyzed to be unimodal, the specificity for illustrating primer very well, can be used for subsequent experimental； If solubility curve is non-unimodal, illustrate the specific bad, it is necessary to redesign primer of primer；Each sample, each pair primer into Row repeats three times, reduces the error in operating process；Using GAPDH as internal reference, i.e. set GAPDH gene expression amounts as 1, use Delta-deltaCt methods carry out relative quantitative assay.

Step 5, the expression quantity according to skin-related gene judges skin condition, and for the selection skin care of user's skin condition Product component and dosage, prepare suitable skin care item.

The effect component of certain content can regulate and control different types of gene expression amount in Skin whitening care cosmetics, so that real Now improve the purpose of skin quality.For example, the skin tune regulated and controled for the colour of skin related gene expression amount such as FLG, MC1R, GSTP1 Manage agent and include Gotu Kola P.E, lycopene, ceramide, lecithin, propolis extract, chamomile extract, ginseng Extract, yeast extract, the one or more of houttuynia extract.And polypeptide then can by adjust in skin with whitening base The purpose of modulin expression is realized because expressing relevant signal path.

Based on above-mentioned principle, the Skin whitening care cosmetics according to the present invention totally include skin conditioning agent, polypeptide and auxiliary agent.Its In, auxiliary agent can include first group of moisturizer, and second group of moisturizer, grease, solvent, preservative, solubilizer, thickener, resists Oxidant, chelating agent, aromatic, anti-sensitizer and water.Totally first group of moisturizing is included for the skin care item that different user is prepared Agent 2-7 parts by weight, second group of moisturizer 2-11 parts by weight, grease 2-11 parts by weight, organic solvent 4-6 parts by weight, preservative 0.2-4 parts by weight, solubilizer 4-6 parts by weight, thickener 2-3 parts by weight, skin conditioning agent 2-10 parts by weight, chelating agent 0.1- 0.4 parts by weight, antioxidant 1-5 parts by weight, polypeptide 0.5-2.5 parts by weight, aromatic 0.02-0.03 parts by weight, anti-sensitizer 0.1-1.5 parts by weight, water 40-70 parts by weight.Wherein,

Moisturizer includes Sodium Hyaluronate, polyglutamic acid, tremella polysaccharides, panaxan, ganoderma lucidum polysaccharide, propane diols, dipropyl Glycol, butanediol, the wherein one or more of glycerine, moisturizer are divided into first group according to the difference of order of addition in preparation process Moisturizer and second group of moisturizer；

Grease includes olive oil, white oil, vaseline, paraffin oil, apricot kernel oil, saualane, macadamia nut oil, jojoba oil, butter The one or more of fruit tree fruit fatty oil；

Organic solvent includes dodecanol, hexadecanol, octadecanol, tadenan, the one or more of cetanol；

Preservative includes Nipasol, iodine propilolic alcohol butyl mephenesin Carbamate, 2- phenoxetols, one kind of methyl hydroxybenzoate It is or a variety of；

Solubilizer include stearic acid, PEG20 rilanit specials, PEG20 stearines, PEG60 rilanit specials, PEG60 iso stearic acid of glycerine ester, Natrulon H-10 stearate, the one or more of PEG/PPG-17/6 copolymers；

Thickener includes hydroxyethyl cellulose, carboxymethyl cellulose, hydroxypropyl guar gum, polymeric acrylic acid esters thickener One or more；

Skin conditioning agent includes Gotu Kola P.E, lycopene, ceramide, lecithin, propolis extract, yellow chamomile Extract, ginseng root extract, yeast extract, the one or more of houttuynia extract；

Chelating agent includes EDETATE SODIUM；

It is more that antioxidant includes brown alga extract, ginseng extract, panaxoside, nutgall extractive, tea polyphenols, ganoderma lucidum Sugar, algal polysaccharides, raspberry extract, resveratrol, avocado oil, grape-kernel oil, astaxanthin, forulic acid, Co-Q10, N- acetyl Cysteine, curcumin, tanshinone IIA, glabridin, the one or more of lipoic acid；

Anti-sensitizer includes dipotassium glycyrrhizinate, hanfangchin A, camomile extract, one kind in Gotu Kola P.E or more Kind；

Aromatic includes the one or more in yulan water extract, aloe water extract, rose water extract；

Polypeptide, the purpose of modulin expression, bag are realized by adjusting with the relevant signal path of gene expression in skin Containing alanine/histidine/lysine polypeptide copper HCl, soybean (GLYCINEMAX) polypeptide, dipeptides -1, dipeptides -2, dipeptides -4, widow Peptide -1, oligopeptides -2, oligopeptides -3, oligopeptides -4, oligopeptides -5, oligopeptides -6, oligopeptides -29, oligopeptides -34, carnosine, saccharomycete polypeptide, essence Propylhomoserin/lysine polypeptide, nonapeptide -1, poly- (tripeptides -6), ascorbyl polypeptide, sodium bacillus subtilis lipopeptide, hexapeptide -1,2,3,5,9, 11st, heptapeptide -6, myristoyl hexapeptide -5, myristoyl pentapeptide -4, tripeptides -1,2,3,10,32, the copper of tripeptides -1, decapeptide -4, four Peptide -1,3,4, pentapeptide -1,3,34, oat peptide, Wild soybean peptide, acetyl group octapeptide -3, -1 cetyl of acetyl group dipeptides, acetyl group six Peptide 1,7,8, acetyl group heptapeptide -4, acetyl group tetrapeptide 2,3,5,9,11, palmityl dipeptides -7, palmityl hexapeptide -12,14,15, palm fibre Palmitic acid acyl tripeptides -1,5,8, palmityl tetrapeptide -5,7,10, one or more of combinations in Matrixyl -4,5.

The preparation method of skin care item totally comprises the steps of：

Step 5a) for the moisturizer of user's skin condition selection appropriate mass ratio of skin care item, grease, organic solvent, prevents Rotten agent, solubilizer, thickener, skin conditioning agent, chelating agent, antioxidant, polypeptide, aromatic, anti-sensitizer and water；

Embodiment 1

The embodiment of the present invention 1 carried out genetic test for the volunteer 1 of 35 years old, and the gene detected includes：The colour of skin is related Gene M C1R, GSTP1, SLC24A5.According to testing result the skin care item of formula are formulated as follows for it：Wherein, skin care item gross mass For common 50Kg,

First group of moisturizer：Sodium Hyaluronate 0.2Kg, polyglutamic acid 0.5Kg, tremella polysaccharides 0.5Kg

Second group of moisturizer：Dipropylene glycol 0.5Kg, glycerine 0.5Kg

Grease：Jojoba oil 0.8Kg, Butyrospermum parkii fruit fatty oil 0.8Kg, vaseline 0.5Kg, paraffin oil 1Kg, saualane 2Kg

Organic solvent：Dodecanol 1Kg, octadecanol 1Kg, tadenan 1Kg

Preservative：2- phenoxetol 1Kg, methyl hydroxybenzoate 0.5Kg

Solubilizer：PEG20 rilanit specials 1Kg, Natrulon H-10 stearate 1Kg

Thickener：Polymeric acrylic acid esters (ice crystal AVC) 1Kg

Skin conditioning agent：Propolis extract 0.5Kg, ginseng root extract 0.1Kg, yeast extract 0.1Kg, cordate houttuynia carry Take thing 0.3Kg

Chelating agent：EDETATE SODIUM 0.05Kg

Antioxidant：Ganoderma lucidum polysaccharide 0.5Kg, ginseng extract 0.2Kg, panaxoside 0.5Kg, tea polyphenols 0.3Kg, five times Seed extract 0.2Kg

Polypeptide：The 0.1Kg of oligopeptides -29, the 0.1Kg of oligopeptides -34, the copper of tripeptides -1 0.2Kg, decapeptide -40.4Kg

Anti-sensitizer：Dipotassium glycyrrhizinate 0.1Kg

Aromatic：Rose water extract 0.02Kg

Other：Water surplus

Volunteer 1 continues to carry out genetic test again after the skin care item using above-mentioned formula in 100 days, it uses above-mentioned skin care Gene relative expression quantity before and after product is as shown in table 5：

Table 5

Gene name	MC1R	GSTP1	SLC24A5	GAPDH
					Before use	1.639588	0.797377	1.013959	1
After use	1.52428	0.737882	0.74506	1
					Reference example	1.211373	1.118944	1.005749	1

Wherein, the reference example of embodiment 1-4 is 100 volunteers each gene relative expression quantity of the age at -30 years old 20 years old Average value.

The expression quantity of gene shows shown in table 5, and the gene M C1R expression quantity before using Skin whitening care cosmetics of volunteer 1 is obvious Higher, ability and ultraviolet resistance is poor, easily tanned.After Skin whitening care cosmetics using above-mentioned formula, gene M C1R, GSTP1, The expression quantity of SLC24A5 substantially reduces, correspondingly, skin resistance UV resistance enhancing.

Embodiment 2

The embodiment of the present invention 2 carried out genetic test for the volunteer 2 of 48 years old, and the gene detected includes：The colour of skin is related Gene M C1R, GSTP1, SLC24A5.According to testing result the skin care item of formula are formulated as follows for it：Wherein, skin care item gross mass For common 50Kg,

First group of moisturizer：Polyglutamic acid 1Kg, tremella polysaccharides 0.5Kg

Second group of moisturizer：Dipropylene glycol 1Kg, glycerine 1.5Kg

Grease：Paraffin oil 1Kg, apricot kernel oil 1Kg, saualane 1Kg, macadamia nut oil 0.8Kg

Organic solvent：Octadecanol 1Kg, cetanol 1Kg

Preservative：Iodine propilolic alcohol butyl mephenesin Carbamate 0.1Kg

Solubilizer：PEG60 rilanit specials 1Kg, PEG60 iso stearic acid of glycerine ester 1Kg

Thickener：Carboxymethyl cellulose 1Kg

Skin conditioning agent：Propolis extract 1Kg, chamomile extract 1Kg

Chelating agent：EDETATE SODIUM 0.1Kg

Antioxidant：Ganoderma lucidum polysaccharide 0.5Kg, tea polyphenols 0.4Kg, brown alga extract 0.2Kg, Co-Q10 0.1Kg

Polypeptide：Alanine/histidine/lysine polypeptide copper HCl 0.1Kg, the 0.1Kg of dipeptides -1, -1 whale of acetyl group dipeptides Wax ester 0.1Kg

Anti-sensitizer：Hanfangchin A 0.05Kg

Aromatic：Aloe water extract 0.01Kg

Other：Water surplus

Volunteer 2 continues to carry out genetic test again after the skin care item using above-mentioned formula in 100 days, it uses above-mentioned skin care Gene relative expression quantity before and after product is as shown in table 6：

Table 6

The expression quantity of gene shows shown in table 6, and the gene M C1R expression quantity before using Skin whitening care cosmetics of volunteer 1 is obvious Relatively low, ability and ultraviolet resistance is preferable.After Skin whitening care cosmetics using above-mentioned formula, the expression of gene M C1R, GSTP1, SLC24A5 Amount reduces, and correspondingly, skin resistance UV resistance further enhances.

Embodiment 3

The embodiment of the present invention 3 carried out genetic test for the volunteer 3 of 43 years old, and the gene detected includes：The colour of skin is related Gene M C1R, GSTP1.According to testing result the skin care item of formula are formulated as follows for it：Wherein, skin care item gross mass is common 50Kg,

First group of moisturizer：Sodium Hyaluronate 0.6Kg, polyglutamic acid 0.4Kg, tremella polysaccharides 0.5Kg, panaxan 1Kg

Second group of moisturizer：Glycerine 3.5Kg

Grease：Saualane 1Kg, macadamia nut oil 1.2Kg, jojoba oil 1.5Kg

Organic solvent：Dodecanol 1.1Kg, tadenan 1.9Kg,

Preservative：Nipasol 0.1Kg, methyl hydroxybenzoate 0.9Kg

Solubilizer：PEG60 rilanit specials 1.1Kg, PEG60 iso stearic acid of glycerine ester 1.2Kg

Thickener：Polymeric acrylic acid esters 1.05Kg

Skin conditioning agent：Ceramide 0.6Kg, propolis extract 0.44Kg, chamomile extract 0.52Kg, yeast extraction Thing 1Kg

Chelating agent：EDETATE SODIUM 0.1Kg

Antioxidant：Resveratrol 0.2Kg, grape-kernel oil 0.2Kg, N-acetylcystein 0.05Kg, glabridin 0.06Kg

Polypeptide：The 0.1Kg of oligopeptides -34, carnosine 0.5Kg, -5 0.4Kg of myristoyl hexapeptide, oat peptide 0.07Kg

Anti-sensitizer：Dipotassium glycyrrhizinate 0.2Kg, Gotu Kola P.E 0.2Kg, hanfangchin A 0.05Kg

Aromatic：Aloe water extract 0.015Kg

Other：Water surplus

Volunteer 3 continues to carry out genetic test again after the skin care item using above-mentioned formula in 100 days, it uses above-mentioned skin care Gene relative expression quantity before and after product is as shown in table 7：

Table 7

Gene name	MC1R	GSTP1	GAPDH
				Before use	1.286395	1.22264	1
After use	1.175848	1.091882	1
				Reference example	1.211373	1.118944	1

The expression quantity of gene shows shown in table 7, volunteer 1 using gene GSTP1 expression quantity before Skin whitening care cosmetics compared with Height, ability and ultraviolet resistance is poor, easily tanned.After Skin whitening care cosmetics using above-mentioned formula, the expression of gene M C1R, GSTP1 Amount is obvious to be reduced, correspondingly, skin resistance UV resistance enhancing.

Embodiment 4

The embodiment of the present invention 4 carried out genetic test for the volunteer 4 of 50 years old, and the gene detected includes：The colour of skin is related Gene M C1R, GSTP1.According to testing result the skin care item of formula are formulated as follows for it：Wherein, skin care item gross mass is common 50Kg,

First group of moisturizer：Polyglutamic acid 1.1Kg, panaxan 1.2Kg, ganoderma lucidum polysaccharide 1Kg

Second group of moisturizer：Dipropylene glycol 1Kg, butanediol 0.4Kg, glycerine 4Kg

Grease：Vaseline 0.2Kg, apricot kernel oil 0.3Kg, saualane 0.5Kg, macadamia nut oil 0.5Kg, jojoba oil 1Kg,

Organic solvent：Tadenan 1.5Kg, cetanol 1Kg

Preservative：2- phenoxetol 1Kg, methyl hydroxybenzoate 1Kg

Solubilizer：PEG60 rilanit specials 1.2Kg, PEG60 iso stearic acid of glycerine ester 1.5Kg

Thickener：Polymeric acrylic acid esters (ice crystal AVC) 1.2Kg

Skin conditioning agent：Cer EOS .4Kg, lecithin 1.2Kg, chamomile extract 1.2Kg, ginseng root extract 1Kg

Chelating agent：EDETATE SODIUM 0.2Kg

Antioxidant：Ginseng extract 1Kg, panaxoside 0.9Kg, tea polyphenols 0.1Kg, curcumin 0.2Kg, Chinese gall carry Take thing 0.2Kg

Polypeptide：The 0.1Kg of tripeptides -3, the 0.1Kg of oligopeptides -29, the 0.15Kg of oligopeptides -34, the 0.15Kg of acetyl group heptapeptide -4

Anti-sensitizer：Dipotassium glycyrrhizinate 0.1Kg, hanfangchin A 0.1Kg, Gotu Kola P.E 0.5Kg aromatic：Aloe water Extract 0.01Kg

Other：Water surplus

Volunteer 4 continues to carry out genetic test again after the skin care item using above-mentioned formula in 100 days, it uses above-mentioned skin care Gene relative expression quantity before and after product is as shown in table 8：

Table 8

Gene name	MC1R	GSTP1	GAPDH
				Before use	0.941696	1.148698	1
After use	0.782848	0.929782	1
				Reference example	1.211373	1.118944	1

The expression quantity of gene shows shown in table 8, volunteer 1 using gene GSTP1 expression quantity before Skin whitening care cosmetics compared with Height, ability and ultraviolet resistance is poor, easily tanned.After Skin whitening care cosmetics using above-mentioned formula, the expression of gene M C1R, GSTP1 Amount is obvious to be reduced, correspondingly, skin resistance UV resistance enhancing.

For the field technology, personnel can understand, above-mentioned specific embodiment can be in the generalized concept without departing substantially from the present invention Change down.Therefore, it should be apparent that the present invention is not limited to disclosed certain specific embodiments, but this is intended to comprising belonging to this Various change in the spirit and scope of invention, i.e., such as appended claim.

SEQUENCE LISTING

<110>Du Libo

<120>A kind of skin care item method for customizing based on genetic test

<130> 1

<160> 12

<170> PatentIn version 3.5

<210> 1

<211> 20

<212> DNA

<213> Artificial Sequence

<220>

<223>GAPDH primers 1

<400> 1

cgggaaactg tggcgtgatg 20

<210> 2

<211> 20

<212> DNA

<213> Artificial Sequence

<220>

<223>GAPDH primer 2s

<400> 2

atgaccttgc ccacagcctt 20

<210> 3

<211> 20

<212> DNA

<213> Artificial Sequence

<220>

<223>MC1R primers 1

<400> 3

cactgcgcta ccacagcatc 20

<210> 4

<211> 20

<212> DNA

<213> Artificial Sequence

<220>

<223>MC1R primer 2s

<400> 4

gcgtgctgaa gacgacactg 20

<210> 5

<211> 20

<212> DNA

<213> Artificial Sequence

<220>

<223>GSTP1 primers 1

<400> 5

gccagagctg gaaggaggag 20

<210> 6

<211> 20

<212> DNA

<213> Artificial Sequence

<220>

<223>GSTP1 primer 2s

<400> 6

gcaggaggct ttgagtgagc 20

<210> 7

<211> 19

<212> DNA

<213> Artificial Sequence

<220>

<223>SLC24A5 primers 1

<400> 7

ctgtgggcca ctgcacatc 19

<210> 8

<211> 19

<212> DNA

<213> Artificial Sequence

<220>

<223>SLC24A5 primer 2s

<400> 8

tggcccttgg gagacgttg 19

<210> 9

<211> 87

<212> DNA

<213> Artificial Sequence

<220>

<223>GAPDH amplified fragments

<400> 9

cgggaaactg tggcgtgatg gccgcggggc tctccagaac atcatccctg cctctactgg 60

cgctgccaag gctgtgggca aggtcat 87

<210> 10

<211> 87

<212> DNA

<213> Artificial Sequence

<220>

<223>MC1R amplified fragments

<400> 10

cactgcgcta ccacagcatc gtgaccctgc cgcgggcgcg gcgagccgtt gcggccatct 60

gggtggccag tgtcgtcttc agcacgc 87

<210> 11

<211> 64

<212> DNA

<213> Artificial Sequence

<220>

<223>GSTP1 amplified fragments

<400> 11

gccagagctg gaaggaggag gtggtgaccg tggagacgtg gcaggagggc tcactcaaag 60

cctc 64

<210> 12

<211> 64

<212> DNA

<213> Artificial Sequence

<220>

<223>SLC24A5 amplified fragments

<400> 12

ctgtgggcca ctgcacatct gcctctctca gggacctccc tgccccaacg tctcccaagg 60

gcca 64

Claims

1. a kind of Skin whitening care cosmetics method for customizing, it is characterised in that comprise the steps of：

Step 1, user's cell sample is gathered；

Step 2, the mRNA in the cell sample is extracted；

Step 3, mRNA reverse transcriptions are synthesized into cDNA；

Step 5, the expression quantity according to the gene pleiomorphism and corresponding cDNA judges whitening capability, and is directed to the whitening capability Selection component and dosage, prepare suitable Skin whitening care cosmetics.

2. Skin whitening care cosmetics method for customizing as claimed in claim 1, it is characterised in that the step 4 includes：

Step 4a), the mRNA sequence of the colour of skin related gene is searched on NCBI, and design corresponding primer；

Step 4b), using delta-deltaCt methods, using gene GAPDH as internal reference, pass through colour of skin phase described in the primer detection The relative expression quantity of the polymorphism of correlation gene and corresponding cDNA.

3. Skin whitening care cosmetics method for customizing as claimed in claim 2, it is characterised in that the primer includes：

GAPDH Forword：5’-CGGGAAACTGTGGCGTGATG-3’

Reverse：5’-ATGACCTTGCCCACAGCCTT-3’

MC1R Forword：5’-CACTGCGCTACCACAGCATC-3’

Reverse：5’-GCGTGCTGAAGACGACACTG-3’

GSTP1 Forword：5’-GCCAGAGCTGGAAGGAGGAG-3’

Reverse：5’-GCAGGAGGCTTTGAGTGAGC-3’

SLC24A5 Forword：5’-CTGTGGGCCACTGCACATC-3’

Reverse：5’-TGGCCCTTGGGAGACGTTG-3’.

4. Skin whitening care cosmetics method for customizing as claimed in claim 1, it is characterised in that the component of the Skin whitening care cosmetics includes Skin conditioning agent, polypeptide and auxiliary agent.

5. Skin whitening care cosmetics method for customizing as claimed in claim 4, it is characterised in that the skin conditioning agent includes centella Extract, lycopene, ceramide, lecithin, propolis extract, chamomile extract, ginseng root extract, yeast extraction The one or more of thing, houttuynia extract.

6. Skin whitening care cosmetics method for customizing as claimed in claim 4, it is characterised in that the polypeptide includes alanine/group ammonia Acid/lysine polypeptide copper HCl, soybean (GLYCINEMAX) polypeptide, dipeptides -1, dipeptides -2, dipeptides -4, oligopeptides -1, oligopeptides -2, widow Peptide -3, oligopeptides -4, oligopeptides -5, oligopeptides -6, oligopeptides -29, oligopeptides -34, carnosine, saccharomycete polypeptide, arginine/lysine are more Peptide, nonapeptide -1, poly- (tripeptides -6), ascorbyl polypeptide, sodium bacillus subtilis lipopeptide, hexapeptide -1,2,3,5,9,11, heptapeptide -6, Pork and beans Cool acyl hexapeptide -5, myristoyl pentapeptide -4, tripeptides -1,2,3,10,32, the copper of tripeptides -1, decapeptide -4, tetrapeptide -1,3,4, pentapeptide -1, 3,34, oat peptide, Wild soybean peptide, acetyl group octapeptide -3, -1 cetyl of acetyl group dipeptides, acetyl group hexapeptide 1,7,8, acetyl group seven Peptide -4, acetyl group tetrapeptide 2,3,5,9,11, palmityl dipeptides -7, palmityl hexapeptide -12,14,15, palmityl tripeptides -1,5,8, Palmityl tetrapeptide -5,7,10, one or more of combinations in Matrixyl -4,5.

7. Skin whitening care cosmetics method for customizing as claimed in claim 4, it is characterised in that the auxiliary agent includes first group of moisturizing Agent, second group of moisturizer, grease, solvent, preservative, solubilizer, thickener, skin conditioning agent, chelating agent, aromatic, resists Quick dose and water.

8. Skin whitening care cosmetics method for customizing as claimed in claim 7, it is characterised in that the step 5 includes：

Step 5a) for user's skin condition selection the appropriate mass ratio of skin care item first group of moisturizer, described second group Moisturizer, the grease, the organic solvent, the preservative, the solubilizer, the thickener, the skin conditioning agent, The chelating agent, the antioxidant, the polypeptide, the aromatic, the anti-sensitizer and water；

Step 5b) solubilizer, the antioxidant, the grease, the organic solvent and the aromatic are being emulsified Hypervelocity is stirred in machine, the mixture A of system；

Step 5d) second group of moisturizer, the chelating agent, the thickener are added in agitated kettle, warming while stirring To 85-95 DEG C, it is fully dissolved, keep the temperature 15-20 minutes, the mixture C of system；

Step 5e) mixture C is cooled to 40-45 DEG C, successively plus the skin conditioning agent, the anti-sensitizer and described more Peptide, stirring and dissolving, the mixture D of system；

Step 5f) in the case where keeping 40-45 DEG C of temperature, add the preservative into the mixture D, it is the mixture A, described Mixture B, be sufficiently stirred, and is down to room temperature, discharging, stand.

9. Skin whitening care cosmetics method for customizing as claimed in claim 8, it is characterised in that the component and matter of the Skin whitening care cosmetics Measuring ratio is：First group of moisturizer 2-7 parts by weight, second group of moisturizer 2-11 parts by weight, grease 2-11 parts by weight, organic solvent 4- 6 parts by weight, preservative 0.2-4 parts by weight, solubilizer 4-6 parts by weight, thickener 2-3 parts by weight, skin conditioning agent 2-10 weight Part, chelating agent 0.1-0.4 parts by weight, antioxidant 1-5 parts by weight, polypeptide 0.5-2.5 parts by weight, aromatic 0.02-0.03 Parts by weight, anti-sensitizer 0.1-1.5 parts by weight, water 40-70 parts by weight.

10. the Skin whitening care cosmetics method for customizing stated such as claim 9, it is characterised in that the component and matter of the Skin whitening care cosmetics Measuring ratio is：First group of 3 parts by weight of moisturizer, second group of 5 parts by weight of moisturizer, 7.6 parts by weight of grease, 4 weight of organic solvent Part, 0.2 parts by weight of preservative, 4 parts by weight of solubilizer, 2 parts by weight of thickener, 4 parts by weight of skin conditioning agent, 0.2 weight of chelating agent Measure part, 2.4 parts by weight of antioxidant, 0.6 parts by weight of polypeptide, 0.02 parts by weight of aromatic, 0.1 parts by weight of anti-sensitizer, water 67 Parts by weight.