CN110819723A

CN110819723A - Detection method for five gene combinations for assessing congenital skin quality and personalized skin care kit formulated according to detection results

Info

Publication number: CN110819723A
Application number: CN201911177460.0A
Authority: CN
Inventors: 饶焕文; 林建平; 叶美娜; 萧自智; 潘发伍; 王希丽
Original assignee: Jiawenli (fujian) Cosmetics Co Ltd
Current assignee: Jiawenli (fujian) Cosmetics Co Ltd
Priority date: 2019-11-27
Filing date: 2019-11-27
Publication date: 2020-02-21

Abstract

A detection method for evaluating the combination of five genes of the native skin and a personalized skin care kit formulated according to the detection result relate to the technical field of beauty and skin care, and aim to provide more targeted and scientific skin care guidance for consumers, and comprise the following steps: step1, detecting the genotype of five gene combinations related to the congenital skin, and judging the congenital skin condition; step2, screening corresponding effect raw materials and designing a skin care formula to make a personalized skin care kit method according to the congenital weak skin problem determined in the Step 1; according to the scheme, the targeted effect raw materials are evaluated and screened according to the detection result by detecting the skin-related gene combination, and the skin beautifying target of efficiently improving the skin quality is really realized by performing the guided design of the accurate skin care scheme according to the current skin situation of the consumer.

Description

Detection method for five gene combinations for assessing congenital skin quality and personalized skin care kit formulated according to detection results

Technical Field

The invention relates to the technical field of beauty and skin care, in particular to a detection method of five gene combinations for evaluating the nature of the innate skin and a personalized skin care kit formulated according to the detection result.

Background

In the traditional skin care mode, the quality of the skin care product is particularly emphasized, and the good skin care product seems to have the effect of maintaining the skin of each person. However, the choice of skin care products by consumers varies from person to person, and the skin care products can be selected according to the skin types and requirements of the consumers to actually care the skin. Therefore, by combining the modern biological detection technology and personalized skin care guidance customization, consumers can more clearly know the nature of their own innate skin, discover the distinctive skin care needs of themselves, and effectively guide the formulation of a specific skin care scheme for the acquired skin.

The gene is the origin of life and is also the cause of human health, beauty and longevity. Scientifically defined genes are DNA (deoxyribonucleic acid) fragments with genetic effects, also called genetic factors, which are the basic genetic units for controlling the traits of organisms. The human genetic material contains various variation types, one of which is called Single Nucleotide Polymorphism (SNP), and the DNA sequence can be read by detecting the type of SNP, so that the inherent skin quality can be accurately known, and the answers of the problems that some people are easy to suntan naturally, easy to be sensitive naturally, high in aging coefficient compared with other people, high in collagen loss risk and the like can be found from gene detection.

Based on the detection of the skin-type related gene SNP locus combination, the corresponding skin care product efficacy raw materials are screened by combining the weak SNP locus in the detection result and the factors such as the current condition, age and region of the skin of a consumer, and the corresponding skin care product kit is configured, so that a more scientific and accurate skin care scheme is formulated for the consumer. This need is at a substantial gap in the industry.

Disclosure of Invention

In order to solve the problems, the invention provides a detection method of five gene combinations for evaluating the congenital skin quality and a personalized skin care kit prepared according to the detection result.

In order to realize the purpose of the invention, the invention adopts the following technical scheme:

an assay for assessing the combination of five genes of the native skin, comprising the steps of: step1, detecting the genotype of five gene combinations related to the congenital skin, including the gene sites related to the congenital antioxidant capacity, the anti-saccharification capacity, the collagen synthesis capacity, the anti-ultraviolet capacity and the inflammation control capacity of the skin; wherein the content of the first and second substances,

the gene related to the innate antioxidant capacity in the five genes related to the innate skin is SOD2, and the effective SNP locus of the gene is RS 4880;

the gene related to the innate anti-glycation ability in the five genes related to the innate skin is AGER, and the effective SNP locus of the gene is RS 1800624;

the gene related to the synthetic ability of the congenital collagen in the five genes related to the congenital skin is MMP3, and the effective SNP site is RS 522616;

the gene related to the inherent ultraviolet resistance in the five genes related to the inherent skin is XRCC1, and the effective SNP locus is RS 2139720;

the gene related to the control capability of the innate inflammation in the five genes related to the innate skin is IL-13, and the effective SNP locus of the gene is RS 848;

and Step2, designing corresponding detection steps according to the detection items in the Step1 to carry out five-gene combined detection, judging the problem of the congenital weak skin by combining the detection result and a database, and guiding the examinee to select the adaptive skin care product raw material.

Further, in Step2, the five gene combination detection Step comprises:

(1) collecting oral mucosa epithelial cells of a subject: gargling with drinking water 20-30 min before collection by a non-invasive oral mucosa epithelial cell collection mode, removing impurities in the oral cavity, wiping the inner side of a cheek by using a cotton swab, scraping 10-20 min below, slightly drying in the air for 1-2min, and putting into a collecting pipe for detection;

(2) extracting a genomic DNA sample of a subject: transferring the cotton swab scraped in the cheek into a 1.5-2 mL centrifuge tube, shearing the cotton swab part from the rod with scissors, adding 300-400 μ L buffer solution, adding 4-5 μ L RNaseA, oscillating for 10-15 s, and standing at room temperature for 3-5 min; adding 10-20 μ L of protease K solution, mixing by vortex for 5-10 s, standing at 50-56 deg.C for 30-60 min, and mixing by vortex for several times every 10-15 min; adding 300-400 μ L buffer solution of precipitated protein, fully reversing and mixing, standing at 60-70 deg.C for 5-10 min; adding 150-200 μ L of anhydrous ethanol, fully reversing and mixing uniformly, and centrifuging briefly to remove liquid drops on the inner wall of the tube;

adding all the obtained solutions into an adsorption column, centrifuging at 10000-12000 rpm for 15-30 s, pouring off waste liquid in a collecting pipe, and putting the adsorption column back into the collecting pipe; adding 400-500 μ L washing buffer solution into adsorption column, centrifuging at 10000-12000 rpm for 15-30 s, pouring off waste liquid in collection tube, and placing adsorption column back into collection tube; adding 500-600 μ L of rinsing liquid into the adsorption column, centrifuging at 10000-12000 rpm for 15-30 s, pouring off waste liquid in the collection tube, and putting the adsorption column back into the collection tube; centrifuging at 10000-12000 rpm for 1-2min, pouring off waste liquid, and standing the adsorption column at room temperature for several minutes to thoroughly air-dry the residual rinsing liquid in the adsorption material; transferring into a clean centrifuge tube, suspending and dripping 20-50 μ L of elution buffer solution into the middle position of the adsorption membrane, standing at room temperature for 2-5min, centrifuging at 10000-12000 rpm for 1-2min, and storing at-20 deg.C for use.

(3) Performing PCR amplification by using fluorescent probe primers and a kit at corresponding sites: wherein, the PCR reaction system is TaqPath ProAmp Master Mix 10.25-12.5. mu.L, 20 xTaqMan SNP Genotyping Assay 1.0. mu.L-1.25. mu.L, Genomic DNA-or-NTC 10.25. mu.L-11.25. mu.L, and nucleic-Free Water is added to 20. mu.L-25. mu.L; the PCR reaction program includes pre-reading at 55-60 deg.c for 20-30 s, pre-denaturing at 95-98 deg.c for 3-5 min, denaturing at 95-98 deg.c for 10-15 s, annealing at 55-60 deg.c for 30-60 s, post-reading at 55-60 deg.c for 20-30 s, and circulating for 35-40 times.

(4) And (3) analyzing a detection experiment result: using Quantstrudio^TMDesign&Analyzing by Analysis Software, and obtaining the genotype of the corresponding SNP locus according to different fluorescence signals.

Further, the specific detection step includes:

(1) collecting oral mucosa epithelial cells of a subject: gargling with drinking water 30min before collection, removing impurities in oral cavity, scraping with cotton swab on the inner side of upper, taking out for 20min, air drying for 2min, and placing into collecting tube for detection;

(2) extracting a genomic DNA sample of a subject: transferring the cotton swab scraped in the cheek into a 2mL centrifuge tube, shearing the cotton swab part from the rod by using scissors, adding 400 mu L buffer solution, adding 4 mu L RNase A, oscillating for 15s, and standing for 5min at room temperature; adding 20 μ L of protease K solution, vortexing for 10s, mixing, standing at 56 deg.C for 60min, and vortexing for 15min for several times; adding 400 μ L buffer solution for precipitating protein, fully reversing, mixing, and standing at 70 deg.C for 10 min; adding 200 μ L of anhydrous ethanol, fully reversing and mixing uniformly, and centrifuging briefly to remove liquid drops on the inner wall of the tube;

adding all the solutions obtained in the previous step into an adsorption column, centrifuging at 12000rpm for 30s, pouring out waste liquid in a collecting pipe, and putting the adsorption column back into the collecting pipe; adding 500 μ L washing buffer solution into adsorption column, centrifuging at 12000rpm for 30s, pouring off waste liquid in the collection tube, and placing adsorption column back into the collection tube; adding 600 μ L of rinsing liquid into the adsorption column, centrifuging at 12000rpm for 30s, pouring off waste liquid in the collection tube, and placing the adsorption column back into the collection tube; centrifuging at 12000rpm for 2min, pouring off waste liquid, and standing the adsorption column at room temperature for several minutes to thoroughly air-dry the residual rinsing liquid in the adsorption material; transferring into a clean centrifuge tube, suspending and dripping 30 μ L elution buffer solution into the middle position of the adsorption membrane, standing at room temperature for 5min, centrifuging at 12000rpm for 2min, and storing at-20 deg.C;

(3) performing PCR amplification by using fluorescent probe primers and a kit at corresponding sites: PCR reaction system is TaqPathProAmp Master Mix 12.5. mu.L, 20 xTaqMan SNP Genotyping Assay 1.25. mu.L, Genomic DNA-or-NTC 11.25. mu.L, nucleic-Free Water added to 25. mu.L; the PCR reaction program comprises pre-reading at 60 deg.C for 30s, pre-denaturing at 95 deg.C for 5min, denaturing at 95 deg.C for 15s, annealing at 60 deg.C for 60s, post-reading at 60 deg.C for 30s, and circulating 40 times.

Further, in the detection step (3), the sequences of five major gene loci are as follows:

further, in the detection step (4), the combined genotypes of the SNP sites of the five major genes are evaluated as follows:

a personalized skin care kit is formulated according to the detection result of a detection method for evaluating the combination of five genes of the congenital skin type, and the skin care product raw materials in the personalized skin care kit are formulated according to the problem of the congenital weak skin, and comprise the following steps:

(1) the skin care product raw material with weak oxidation resistance aiming at the native skin is one or more of fullerene, roselle flower extract, coffee mallow fruit extract, hyaluronic acid, gardenia fruit extract and nutgrass flatsedge extract.

(2) The skin care product raw material with weak anti-saccharification capacity aiming at the native skin is one or more of sunflower seed extract, silybum marianum extract, tea polyphenol, carnosine, evening primrose extract and candida hydrolysate extract.

(3) The skin care product raw material with weak synthesis capability of the native skin collagen is one or more of snake venom peptide, carved line peptide, blue copper peptide, signal peptide and oriental cherry extract.

(4) The raw materials of the skin care product aiming at weak ultraviolet resistance of the native skin are one or more of papain, mandelic acid, photinia glabra extract, glycogen, bismuth oxychloride, oriental cherry extract, nicotinamide, whitening nonapeptide and undaria pinnatifida extract.

(5) The skin care product with weak control capability on congenital skin inflammation is prepared from one or more of asiaticoside, carboxymethyl chitosan, tamarind seed polysaccharide, radix Rhodiolae extract, herba Avenae Fatuae kernel extract, radix Scutellariae extract, herba Polygoni Hydropiperis extract, radix Sophorae Flavescentis extract, herba Elsholtziae Fruticosae extract, folium Hibisci Mutabilis extract and quercetin.

Further, the skin care product raw materials are added into cosmetic water, emulsion, essence, cream and a facial mask phase of a basic formula as functional raw materials, wherein the specific addition ratio of the functional raw materials in the basic formula comprises the following components:

(1) aiming at the ratio of skin care product raw materials with weak oxidation resistance of the innate skin in each skin care product phase, the fullerene/basic formula is 0.1-1%, the roselle flower extract/basic formula is 0.5-1%, the coffee abelmoschus esculentus fruit extract/basic formula is 0.1-1%, the hyaluronic acid/basic formula is 1-2%, the gardenia fruit extract/basic formula is 0.5-1%, and the sedge extract/basic formula is 0.2-1%.

(2) According to the proportion of skin care product raw materials with weak anti-saccharification capacity of the innate skin in each skin care product phase, the sunflower seed extract/basic formula is 0.2-1%, the silybum marianum extract/basic formula is 0.01-0.5%, the tea polyphenol/basic formula is 0.5-1%, the carnosine/basic formula is 0.02-0.5%, the evening primrose extract/basic formula is 0.1-0.5%, and the candida hydrolysate extract/basic formula is 0.2-1%.

(3) Aiming at the ratio of skin care product raw materials with weak synthesis capability of native skin collagen in each skin care product phase, the snake venom peptide/basic formula is 0.01-1%, the line-carved peptide/basic formula is 0.05-0.1%, the blue copper peptide/basic formula is 0.01-0.5%, the signal peptide/basic formula is 0.05-1%, and the oriental cherry flower extract/basic formula is 0.5-1%.

(4) Aiming at the ratio of skin care product raw materials with weak ultraviolet resistance of the innate skin in each skin care product phase, 0.01-0.05 percent of papain/basic formula, 0.02-0.1 percent of mandelic acid/basic formula, 0.1-0.5 percent of photinia glabra extract/basic formula, 0.1-1 percent of glycogen/basic formula, 0.05-1 percent of bismuth oxychloride/basic formula, 0.5-2 percent of oriental cherry extract/basic formula, 0.5-2 percent of nicotinamide/basic formula, 0.02-0.5 percent of whitening nonapeptide/basic formula and 0.05-2 percent of undaria pinnatifida extract/basic formula.

(5) Aiming at the ratio of skin care product raw materials with weak control capability on the inflammation of the native skin in each skin care product phase, asiaticoside/basic formula is 0.35-0.5%, carboxymethyl chitosan/basic formula is 0.05-0.1%, tamarind seed polysaccharide/basic formula is 0.02-0.5%, rhodiola root extract/basic formula is 0.01-1%, oat kernel extract/basic formula is 0.02-0.5%, scutellaria root extract/basic formula is 1-1.25%, 0.02-0.1 percent of polygonum hydropiper extract/basic formula, 0.05-0.5 percent of picrashiza kurroa root extract/basic formula, 0.1-1 percent of ardisia japonica extract/basic formula, 0.5-1 percent of cotton rose leaf extract/basic formula and 0.03-0.5 percent of quercetin/basic formula.

Further, the preferable adding proportion of the efficacy raw materials in the basic formula comprises the following steps:

(1) preferably, the addition ratio of the fullerene/basic formula is 0.5%, the roselle flower extract/basic formula is 1%, the coffee abelmoschus fruit extract/basic formula is 0.6%, the hyaluronic acid/basic formula is 1.5%, the gardenia fruit extract/basic formula is 0.8%, and the sedge extract/basic formula is 0.5% to the skin care product phase with weak oxidation resistance of the native skin.

(2) For the addition ratio of the skin care product raw materials with weak anti-glycation capability of the native skin in each skin care product phase, the preferred ratio is 0.35% of sunflower seed extract/basic formula, 0.05% of silybum marianum extract/basic formula, 0.6% of tea polyphenol/basic formula, 0.25% of carnosine/basic formula, 0.35% of evening primrose extract/basic formula and 0.8% of candida hydrolytica extract/basic formula.

(3) Preferably, the ratio of snake venom peptide/basic formula is 0.06%, the ratio of line-carved peptide/basic formula is 0.45%, the ratio of blue copper peptide/basic formula is 0.5%, the ratio of signal peptide/basic formula is 0.2%, and the ratio of oriental cherry extract/basic formula is 0.8%, aiming at the addition ratio of skin care product raw materials with weak synthesis ability of native skin collagen in each skin care product phase.

(4) Preferably, the ratio of papain/basic formula is 0.05%, the ratio of mandelic acid/basic formula is 0.08%, the ratio of photinia glabra extract/basic formula is 0.3%, the ratio of glycogen/basic formula is 1%, the ratio of bismuth oxychloride/basic formula is 0.75%, the ratio of oriental cherry extract/basic formula is 1.5%, the ratio of nicotinamide/basic formula is 1.35%, the ratio of whitening nonapeptide/basic formula is 0.45%, and the ratio of Undaria pinnatifida extract/basic formula is 1.5%.

(5) Preferably, for the addition ratio of the skin care product raw materials with weak native skin inflammation control ability in each skin care product phase, the asiaticoside/basic formula is 0.5%, the carboxymethyl chitosan/basic formula is 0.1%, the tamarind seed polysaccharide/basic formula is 0.02%, the rhodiola root extract/basic formula is 0.9%, the oat kernel extract/basic formula is 0.3%, the scutellaria root extract/basic formula is 1.25%, the polygonum hydropiper extract/basic formula is 0.08%, the sophora flavescens root extract/basic formula is 0.25%, the ardisia japonica extract/basic formula is 0.5%, the cotton rose leaf extract/basic formula is 0.5%, and the quercetin/basic formula is 0.15%.

By adopting the technical scheme, the corresponding skin care product efficacy raw materials are screened by combining weak SNP sites in detection results and factors such as current conditions, ages and regions of the skin of a consumer based on detection of skin-type related gene SNP site combinations, and corresponding skin care product kit configuration is carried out, so that a more scientific and accurate skin care scheme is formulated for the consumer.

Detailed Description

The present invention is further illustrated by the following detailed description, which only describes some embodiments of the present invention and does not limit the content of the present invention. Other embodiments, which can be made by persons skilled in the art without any inventive contribution, fall within the scope of protection of the present invention.

An assay for assessing the combination of five genes of the native skin, comprising the steps of:

step1, detecting the genotype of five gene combinations related to the congenital skin, including the gene sites related to the congenital antioxidant capacity, the anti-saccharification capacity, the collagen synthesis capacity, the anti-ultraviolet capacity and the inflammation control capacity of the skin; wherein the content of the first and second substances,

Further, in Step2, the five gene combination detection Step comprises:

The above steps are further illustrated by the following specific experimental data:

EXAMPLE 1 establishment of the protocol

Step1 screening the relationship between human skin and genetic gene according to molecular mechanism research, literature retrieval and clinical data analysis, and determining five skin-related gene sites including susceptibility genes and SNP sites related to skin oxidation resistance, anti-saccharification capacity, collagen synthesis capacity, anti-ultraviolet capacity and inflammation control capacity, wherein the specific genes and SNP sites are as follows:

serial number	Item	Gene	SNP site
				1	Antioxidant capacity	SOD2	RS4880
2	Anti-glycation ability	AGER	RS1800624
				3	Ability to synthesize collagen	MMP3	RS522616
4	Ultraviolet resistance	XRCC1	RS2139720
				5	Ability to control inflammation	IL13	RS848

Step2, synthesizing a set of primer pairs with fluorescent probes according to the polymorphism of each site, wherein the specific site polymorphism is as follows:

example 2 sample extraction and analysis

Step1 sample collection: gargling with drinking water 30min before collection by a noninvasive collection mode of oral mucosa epithelial cells, removing impurities in the oral cavity, wiping the inner side of a cheek by using a cotton swab, taking the swab for 20 minutes, slightly airing in the air for 1-2min, and then putting the swab into a collecting pipe for detection, wherein the number of collected samples is 5.

Step2 sample DNA extraction: the oral epithelial cell DNA is extracted by utilizing an oral swab genome DNA extraction kit of Tiangen biochemical technology. Firstly, adding a certain amount of absolute ethyl alcohol into a buffer GD and a rinsing liquid PW, transferring a cotton swab scraped in a cheek into a 2mL centrifuge tube, shearing the cotton swab part from a rod of the cotton swab part by using scissors, adding 400 mu L of buffer GA, adding 4 mu L of RNase A (100mg/mL), oscillating for 15s, and standing for 5min at room temperature; adding 20 μ L of protease K solution, vortexing for 10s, mixing, standing at 56 deg.C for 60min, and vortexing for 15min for several times; adding 400 μ L buffer GB, fully reversing and mixing, standing at 70 deg.C for 10 min; adding 200 μ L of anhydrous ethanol, fully reversing and mixing uniformly, and centrifuging briefly to remove liquid drops on the inner wall of the tube; adding all the solution obtained in the previous step into an adsorption column CR2 (the adsorption column CR2 is put into a collecting pipe), centrifuging for 30s at 12000rpm (about 13400Xg), pouring waste liquid in the collecting pipe, and putting the adsorption column CR2 back into the collecting pipe; adding 500 mu L of buffer GD into an adsorption column CR2, centrifuging at 12000rpm (-13400 Xg) for 30s, pouring waste liquid in a collecting tube, and putting the adsorption column back into the collecting tube; adding 600 mu L of rinsing liquid PW into the adsorption column, centrifuging at 12000rpm (-13400 xg) for 30s, pouring the waste liquid in the collecting pipe, and putting the adsorption column back into the collecting pipe; centrifuging at 12000rpm (-13400 xg) for 2min, pouring off waste liquid, and placing the adsorption column at room temperature for several minutes to completely dry the residual rinsing liquid in the adsorption material; transferring the adsorption column into a clean centrifuge tube, suspending and dripping 20-50 μ L of elution buffer TB into the middle position of the adsorption membrane, standing at room temperature for 2-5min, centrifuging at 12000rpm (-13400 Xg) for 2min, and storing at-20 ℃ for later use.

Step3 PCR amplification: PCR reaction system is TaqPath ProAmp Master Mix (Applied Biosystems, Applied Biotechnology Co., Ltd.) 12.5. mu.L, TaqMan SNP Genotyping Assay [20x ] (Applied Biosystems ) 1.25. mu.L, Genomic DNA-or-NTC 11.25. mu.L, nucleic-Free Water (Thermo Scientific, Semmer Feichal technology) added to 25. mu.L; the PCR reaction program comprises pre-reading at 60 deg.C for 30s, pre-denaturing at 95 deg.C for 5min, denaturing at 95 deg.C for 15s, annealing at 60 deg.C for 60s, post-reading at 60 deg.C for 30s, and circulating for 40 times; the detection instrument is a QuantStaudio 3 real-time fluorescence quantitative PCR system (Applied Biosystems, Applied Biotechnology, Inc., USA).

Example 3 interpretation of Gene test results

The detection results of 25 SNP locus genes of 5 samples in example 2 are analyzed, skin types of the detected samples are analyzed by combining with SNP locus typing significance interpretation of skin-related susceptibility genes, and the specific detection results and interpretation analysis are as follows:

a first sample:

sample two:

sample three:

sample four:

sample five:

example 4 efficacy materials screening

Step1 analyzes according to the interpretation of gene detection result, the functional raw materials with weak oxidation resistance include one or more of fullerene, roselle flower extract, coffee mallow fruit extract, hyaluronic acid, gardenia fruit extract and nutgrass flatsedge extract.

Step2 analyzes according to the interpretation of gene detection result, and the corresponding functional raw materials with weak anti-saccharification capacity comprise one or more of sunflower seed extract, silybum marianum extract, tea polyphenol, carnosine, evening primrose extract and candida hydrolysate extract.

Step3 according to the interpretation analysis of gene detection result, the functional raw materials with weaker collagen synthesis ability comprise one or more of snake venom peptide, carved line peptide, copper blue peptide, signal peptide and oriental cherry flower extract.

Step4 is analyzed according to gene detection result, and the corresponding functional raw materials with weak ultraviolet resistance comprise one or more of papain, mandelic acid, Photinia glabra extract, glycogen, bismuth oxychloride, oriental cherry extract, nicotinamide, whitening nonapeptide and Undaria pinnatifida extract.

Step5 analyzes according to gene detection result, and the corresponding functional material with weak inflammation control ability comprises one or more of asiaticoside, carboxymethyl chitosan, semen Phaseoli Radiati polysaccharide, radix Rhodiolae extract, herba Avenae Fatuae extract, Scutellariae radix extract, herba Polygoni Hydropiperis extract, radix Sophorae Flavescentis extract, herba Asplenii Incisi extract, folium Hibisci Mutabilis extract and quercetin.

The personalized skin care kit is formulated according to the detection result, and the skin care product raw materials in the personalized skin care kit are formulated according to the problem of inherent weak skin, and comprise:

(1) the skin care product raw material with weak oxidation resistance aiming at the native skin is one or more of fullerene, roselle flower extract, coffee mallow fruit extract, hyaluronic acid, gardenia fruit extract and nutgrass flatsedge extract. Wherein the Gardenia fruit extract is a strong antioxidant, and has effects of resisting skin premature aging and resisting blue light; the Cyperus rotundus L.extract has antioxidant and antiinflammatory effects; the fullerene has stable oxidation resistance, has a football-shaped structure with higher stability, can be compatible with free groups such as active oxygen in the skin without deformation, and can improve the self-resistance of the skin.

(2) The skin care product raw material with weak anti-saccharification capacity aiming at the native skin is one or more of sunflower seed extract, silybum marianum extract, tea polyphenol, carnosine, evening primrose extract and candida hydrolysate extract. Wherein carnosine has the effect of quenching the glycosylated end product; the hydrolyzed candida extract can activate and enhance an autophagy system in the skin, enhance the degradation capability of lysosomes to toxic proteins and dead cells, and thus repair damaged skin.

(3) The skin care product raw material aiming at weak synthesis capability of native skin collagen is one or more of snake venom peptide, line carving peptide, blue copper peptide, signal peptide and cherry blossom extract. Wherein the signal peptide (palmitoyl pentapeptide-4 + arginine/lysine polypeptide) regulates the activity of cells through the interaction with a special receiver on a cell membrane, activates the gene activity related to the renewal of extracellular matrix and the proliferation of cells so as to promote the skin to synthesize more collagen, hyaluronic acid and other substances, reduce wrinkles, increase the elasticity of the skin and effectively reverse the aging; in daily life, due to stimulation of various external and internal factors, excessive neuronal cells in the skin release neurotransmitters, frequent repeated contraction and relaxation of muscles can shorten the life of elastic fibers, so that collagen is aged in advance, and the skin is aged in advance due to long-term accumulation, so that the thread carving peptide (acetyl hexapeptide-8 + dipeptide diaminobutyrylbenzylamide diacetate) can strongly inhibit muscle contraction to reduce the generation of wrinkles; the blue copper peptide (tripeptide-1 copper) recovers the skin repairing capability, increases the generation of intercellular mucilage of the skin, reduces the skin damage, stimulates the formation of glucose polyamine, increases the skin thickness, reduces the skin looseness, compacts the skin, stimulates the formation of collagen and elastin, compacts the skin and reduces wrinkles; the oriental cherry flower extract contains polysaccharide, organic acid, etc., can provide nutrition for cells, and has shaping effect.

(4) The skin care product aiming at weak ultraviolet resistance of the congenital skin is prepared from one or more of papain, mandelic acid, photinia glabra extract, glycogen, bismuth oxychloride, cherry blossom extract, nicotinamide, whitening nonapeptide and undaria pinnatifida extract, wherein the papain is cysteine protease and can promote the proteolysis of the outer layer of the horny layer, and the adhesion among keratinocytes is broken by an enzymolysis method, so that the peeling and the falling of the keratinocytes are promoted, the skin is softened, the mandelic acid can inhibit the activity of the tyrosine, prevent and block the generation of the melanin, remove old cutin, fade the melanin, and the phenomenon of dark spots and the like formed by mild metabolism can be avoided, the photinia glabra extract has the effects of clearing away heat and toxic materials, relieving swelling, removing blood stasis, improving blood vessel microcirculation and the like, the glycogen can promote the skin to generate collagen, glycosaminoglycan and fibroblasts by increasing the metabolism of cells, can quickly permeate into the skin, the hyaluronic acid can be combined with water, the moisturizing performance of the hyaluronic acid is enhanced, the skin is smooth and the pigmentation is lightened, the surface of the bismuth oxychloride molecule structure is regular octagon, the surface of the melanin, the melanin is inhibited, the melanin is high in the refractive index of the melanin, the melanin is inhibited, the melanin migration of the melanin migration to the melanin-1-7-melanin formation of the melanin, the.

(5) The skin care product with weak control capability on congenital skin inflammation is prepared from one or more of asiaticoside, carboxymethyl chitosan, tamarind seed polysaccharide, radix Rhodiolae extract, herba Avenae Fatuae kernel extract, radix Scutellariae extract, herba Polygoni Hydropiperis extract, radix Sophorae Flavescentis extract, herba Elsholtziae Fruticosae extract, folium Hibisci Mutabilis extract and quercetin. Wherein asiaticoside can promote synthesis of fibrin and collagen, and has anti-inflammatory effect; the carboxymethyl chitosan is a natural polysaccharide source, promotes the formation of cells of the skin stratum corneum, and accelerates the repair of the skin stratum corneum; the sour bean seed polysaccharide can resist the invasion of harmful bacteria by promoting the skin to secrete antibacterial peptide; the radix Rhodiolae extract can inhibit release of histamine and interferon-gamma, and reduce skin inflammation; oat kernel extract can inhibit histamine release, and has rapid anti-inflammatory and antipruritic activity; the radix Scutellariae extract can resist the release of inflammatory mediators and inhibit the damage of inflammatory mediators to normal cells; the extract of Ardisia obovata Thunb inhibits phospholipase A2 and cyclooxygenase (both inflammatory factors) and reduces inflammation and allergic reaction; the quercetin has effects of resisting irritation, resisting allergy, relieving skin, improving skin self-protecting ability, and reducing irritation; the polygonum hydropiper extract improves the reaction starting critical value of sensitive skin and increases the skin tolerance; the extract of the sophora flavescens roots can reduce the phenomena of skin sensitivity and pruritus and increase the tolerance of the skin; the folium Hibisci Mutabilis extract has strong antioxidant and antiinflammatory effects.

Further, the skin care product raw materials are added into cosmetic water, emulsion, essence, cream and mask product phases of a basic formula as functional raw materials, and a personalized skin care kit is designed according to a corresponding skin care formula, wherein the personalized skin care kit comprises an anti-aging kit, an anti-saccharification kit, a tightening and molding kit, a whitening kit and an anti-allergy kit. Wherein the specific addition proportion of the efficacy raw materials in the basic formula comprises the following steps:

According to the invention, based on the detection of the SNP locus combination of the skin-type related gene, the weak SNP locus in the detection result and the factors such as the current condition, age and region of the skin of a consumer are combined to screen the corresponding skin care product efficacy raw materials, and the corresponding skin care product kit is configured, so that a more scientific and accurate skin care scheme is formulated for the consumer.

A first sample:

sample two:

sample three:

sample four:

sample five:

while the foregoing description shows and describes the preferred embodiments of the present invention, it is to be understood that the invention is not limited to the forms disclosed herein, but is not to be construed as excluding other embodiments and is capable of use in various other combinations, modifications, and environments and is capable of changes within the scope of the inventive concept as described herein, commensurate with the above teachings, or the skill or knowledge of the relevant art. And that modifications and variations may be effected by those skilled in the art without departing from the spirit and scope of the invention as defined by the appended claims.

Claims

1. A test method for evaluating the combination of five genes of the congenital skin type is characterized in that: the method comprises the following steps:

the gene related to the synthetic ability of the congenital collagen in the five genes related to the congenital skin is MMP3, and the effective SNP locus of the gene is RS 522616;

2. The test method for assessing the combination of five genes of the innate skin according to claim 1, wherein: in Step2, the five gene combination detection Step comprises:

(2) extracting a genomic DNA sample of a subject: transferring the cotton swab scraped in the cheek into a 1.5-2 mL centrifuge tube, shearing the cotton swab part from the rod by using scissors, adding 300-400 muL buffer solution, adding 4-5 muL RNase A, oscillating for 10-15 s, and standing at room temperature for 3-5 min; adding 10-20 μ L of protease K solution, mixing by vortex for 5-10 s, standing at 50-56 deg.C for 30-60 min, and mixing by vortex for several times every 10-15 min; adding 300-400 μ L buffer solution of precipitated protein, fully reversing and mixing, standing at 60-70 deg.C for 5-10 min; adding 150-200 μ L of anhydrous ethanol, fully reversing and mixing uniformly, and centrifuging briefly to remove liquid drops on the inner wall of the tube;

adding all the obtained solutions into an adsorption column, centrifuging at 10000-12000 rpm for 15-30 s, pouring off waste liquid in a collecting pipe, and putting the adsorption column back into the collecting pipe; adding 400-500 μ L washing buffer solution into adsorption column, centrifuging at 10000-12000 rpm for 15-30 s, pouring off waste liquid in collection tube, and placing adsorption column back into collection tube; adding 500-600 μ L of rinsing liquid into the adsorption column, centrifuging at 10000-12000 rpm for 15-30 s, pouring off waste liquid in the collection tube, and putting the adsorption column back into the collection tube; centrifuging at 10000-12000 rpm for 1-2min, pouring off waste liquid, and standing the adsorption column at room temperature for several minutes to thoroughly air-dry the residual rinsing liquid in the adsorption material; transferring into a clean centrifuge tube, suspending and dripping 20-50 μ L of elution buffer solution into the middle position of the adsorption membrane, standing at room temperature for 2-5min, centrifuging at 10000-12000 rpm for 1-2min, and storing at-20 deg.C;

(3) performing PCR amplification by using fluorescent probe primers and a kit at corresponding sites: wherein, the PCR reaction system is TaqPathProAmp Master Mix 10.25-12.5. mu.L, 20 xTaqMan SNP Genotyping Assay 1.0. mu.L-1.25. mu.L, Genomic DNA-or-NTC 10.25. mu.L-11.25. mu.L, and nucleic-Free Water is added to 20. mu.L-25. mu.L; the PCR reaction program comprises the steps of firstly reading at 55-60 ℃ for 20s-30s, pre-denaturing at 95-98 ℃ for 3min-5min, denaturing at 95-98 ℃ for 10s-15s, annealing at 55-60 ℃ for 30s-60s, then reading at 55-60 ℃ for 20s-30s, and circulating for 35-40 times;

3. The test method for assessing the combination of five genes of the innate skin according to claim 2, wherein: the specific detection steps include:

(3) performing PCR amplification by using fluorescent probe primers and a kit at corresponding sites: PCR reaction system is TaqPathProAmp Master Mix 12.5. mu.L, 20 xTaqMan SNP Genotyping Assay 1.25. mu.L, Genomic DNA-or-NTC 11.25. mu.L, nucleic-Free Water added to 25. mu.L; the PCR reaction program comprises reading at 60 deg.C for 30s, pre-denaturing at 95 deg.C for 5min, denaturing at 95 deg.C for 15s, annealing at 60 deg.C for 60s, reading at 60 deg.C for 30s, and circulating for 40 times;

4. The test method for assessing the combination of five genes of the innate skin according to claim 2, wherein: in the detection step (3), the sequences of five gene loci are as follows:

5. the test method for assessing the combination of five genes of the innate skin according to claim 2, wherein: in the detection step (4), the combined genotypes of the SNP loci of the five genes are evaluated as follows:

6. the test result of the test method for assessing the five major gene combinations of the innate skin according to any one of claims 1 to 5 formulating a personalized skin care kit, characterized in that: aiming at the problem of inherent weak skin, a personalized skin care kit is prepared, and different raw materials are added into a basic formula skin care product, wherein the raw materials comprise:

(1) the skin care product raw material with weak oxidation resistance aiming at the native skin is one or more of fullerene, roselle flower extract, coffee mallow fruit extract, hyaluronic acid, gardenia fruit extract and nutgrass flatsedge extract;

(2) the skin care product raw material with weak anti-saccharification capacity aiming at the native skin is one or more of sunflower seed extract, silybum marianum extract, tea polyphenol, carnosine, evening primrose extract and candida hydrolysate extract;

(3) aiming at the skin care product raw material with weak synthesis capability of native skin collagen, the skin care product raw material is one or more of snake venom peptide, line carving peptide, blue copper peptide, signal peptide and oriental cherry flower extract;

(4) aiming at skin care products with weak ultraviolet resistance of the native skin, the raw materials are one or more of papain, mandelic acid, photinia glabra extract, glycogen, bismuth oxychloride, oriental cherry extract, nicotinamide, whitening nonapeptide and undaria pinnatifida extract;

7. The customized personalized skin care kit of claim 6, wherein: the skin care product raw materials are added into cosmetic water, emulsion, essence, cream and a facial mask product phase of a basic formula as functional raw materials, wherein the specific addition ratio of the functional raw materials in the basic formula comprises the following components:

(1) aiming at the ratio of skin care product raw materials with weak oxidation resistance of the innate skin in each skin care product phase, 0.1-1% of fullerene/basic formula, 0.5-1% of roselle flower extract/basic formula, 0.1-1% of coffee abelmoschus esculentus fruit extract/basic formula, 1-2% of hyaluronic acid/basic formula, 0.5-1% of gardenia fruit extract/basic formula and 0.2-1% of nutgrass flatsedge extract/basic formula;

(2) aiming at the proportion of skin care product raw materials with weak anti-saccharification capacity of the innate skin in each skin care product phase, 0.2 to 1 percent of sunflower seed extract/basic formula, 0.01 to 0.5 percent of silybum marianum extract/basic formula, 0.5 to 1 percent of tea polyphenol/basic formula, 0.02 to 0.5 percent of carnosine/basic formula, 0.1 to 0.5 percent of evening primrose extract/basic formula and 0.2 to 1 percent of candida hydrolysate extract/basic formula;

(3) aiming at the ratio of skin care product raw materials with weak synthesis capability of native skin collagen in each skin care product phase, 0.01-1% of snake venom peptide/basic formula, 0.05-0.1% of line-carved peptide/basic formula, 0.01-0.5% of bluestone peptide/basic formula, 0.05-1% of signal peptide/basic formula and 0.5-1% of oriental cherry flower extract/basic formula;

(4) aiming at the ratio of skin care product raw materials with weak ultraviolet resistance of the innate skin in each skin care product phase, 0.01-0.05 percent of papain/basic formula, 0.02-0.1 percent of mandelic acid/basic formula, 0.1-0.5 percent of photinia glabra extract/basic formula, 0.1-1 percent of glycogen/basic formula, 0.05-1 percent of bismuth oxychloride/basic formula, 0.5-2 percent of oriental cherry extract/basic formula, 0.5-2 percent of nicotinamide/basic formula, 0.02-0.5 percent of whitening nonapeptide/basic formula and 0.05-2 percent of undaria pinnatifida extract/basic formula;

8. The customized personalized skin care kit of claim 7, wherein: the preferable adding proportion of the functional raw materials in the basic formula comprises the following components:

(1) preferably, the addition ratio of the fullerene/basic formula to the skin care product phase is 0.5%, the roselle flower extract/basic formula to 1%, the coffee abelmoschus fruit extract/basic formula to 0.6%, the hyaluronic acid/basic formula to 1.5%, the gardenia fruit extract/basic formula to 0.8%, and the sedge extract/basic formula to 0.5%;

(2) preferably, the addition ratio of the sunflower seed extract/basic formula is 0.35%, the silybum marianum extract/basic formula is 0.05%, the tea polyphenol/basic formula is 0.6%, the carnosine/basic formula is 0.25%, the evening primrose extract/basic formula is 0.35%, and the candida hydrolyticus extract/basic formula is 0.8% for the skin care product raw materials with weak anti-glycation capability of the native skin;

(3) preferably, the adding proportion of the snake venom peptide/basic formula is 0.06%, the thread carving peptide/basic formula is 0.45%, the blue copper peptide/basic formula is 0.5%, the signal peptide/basic formula is 0.2%, and the oriental cherry extract/basic formula is 0.8% in allusion to the adding proportion of the skin care product raw materials with weak synthesis capability of the native skin collagen in each skin care product phase;

(4) aiming at the adding proportion of skin care product raw materials with weak ultraviolet resistance of the innate skin in each skin care product phase, the preferable formula is 0.05 percent of papain/basic formula, 0.08 percent of mandelic acid/basic formula, 0.3 percent of photinia glabrata extract/basic formula, 1 percent of glycogen/basic formula, 0.75 percent of bismuth oxychloride/basic formula, 1.5 percent of oriental cherry flower extract/basic formula, 1.35 percent of nicotinamide/basic formula, 0.45 percent of whitening nonapeptide/basic formula and 1.5 percent of undaria pinnatifida extract/basic formula;