CN111773155B

CN111773155B - Active matter stabilizing peptide containing fermentation source

Info

Publication number: CN111773155B
Application number: CN202010663926.4A
Authority: CN
Inventors: 侯亚东; 王国柱; 李莹; 张晓荣; 王燕
Original assignee: Shaanxi HuiKang Bio Tech Co Ltd
Current assignee: Shaanxi HuiKang Bio Tech Co Ltd
Priority date: 2020-07-10
Filing date: 2020-07-10
Publication date: 2022-11-04
Anticipated expiration: 2040-07-10
Also published as: CN111773155A

Abstract

The invention discloses an active matter stabilizing peptide from fermentation sources, which is prepared from the following components in percentage by weight: 10-25% of sodium surfactin, 184-5% of poloxamer, 1-3% of cyclodextrin, 10-15% of PEG-400, 11-22% of polyalcohol, 0.1-1.5% of anti-wrinkle peptide compound, 0.1-0.5% of plant bacteriostatic agent, 1-4% of purslane extract and the balance of deionized water. The stable peptide is transparent and colorless, has pH = 5-6, is close to the pH value of skin, has a nano-scale particle size, can solve the stability factor of the peptide, has lower cost than freeze-dried powder, has the functions of permeation promotion and slow release, can effectively exert the efficacy of the peptide raw material, increases the transdermal absorption of the peptide raw material, is used for multiple times at low concentration, is suitable for developing anti-aging repair products of sensitive muscles, and can be added into product systems of various formulations, including face cleaning, facial masks, smoothing toner, essence, emulsion, cream and the like.

Description

Stable peptide containing active matter from fermentation source

Technical Field

The invention belongs to the technical field of cosmetics, and particularly relates to an active matter stabilizing peptide containing a fermentation source.

Background

The rapid development and wide application of modern science and technology bring brand new development opportunities to the beauty and cosmetics industry, and skin care products are developed from chemical beauty and plant beauty to biological beauty and gene beauty. Therefore, peptides (also called polypeptides) with significant cosmetic efficacy are produced. The use history of the polypeptide has been over 20 years, the polypeptide has been widely applied abroad, and the polypeptide cosmetics are introduced by a plurality of national well-known brands. The functional cosmetic of polypeptides is gradually being beautiful, and the functional components, especially the polypeptide additives, play an increasingly important role in the safety, stability, easy absorption, good effect and the like. The peptides have biological characteristics, can fundamentally improve and repair various problems of skin when being added into cosmetics, and particularly have obvious effects on the aspects of supplementing skin nutrition, resisting wrinkles, resisting aging and the like.

However, small molecule peptides are extremely unstable in the application process, and hydrolysis, oxidation, flocculation and other phenomena occur due to factors such as pH (pH value), temperature, microorganisms, oxygen and the like, so that the biological activity disappears, and the efficacy is not the same. Cosmetic manufacturers have also sought a number of stable processes, such as the processing of products in the form of lyophilized powders according to pharmaceutical standards, which are also popular in recent years, but relatively costly and prohibitive to some consumers. Therefore, the peptide cosmetics are gradually developing to a multidimensional mode of 'stability + safety + efficacy + price for the people' and have very wide application prospects.

Disclosure of Invention

In view of the deficiencies of the prior art, it is an object of the present invention to provide a stabilized peptide comprising an active from fermentation. Aiming at the purposes, the stable peptide containing the active matters from fermentation sources adopted by the invention comprises the following components in percentage by weight:

10-25% of sodium surfactin, 3-5% of poloxamer 184, 1-3% of cyclodextrin, 10-15% of PEG-400, 11-22% of polyol, 0.1-1.5% of anti-wrinkle peptide compound, 0.1-0.5% of plant bacteriostatic agent, 1-4% of purslane extract and the balance of deionized water.

The active matter stabilizing peptide containing fermentation source preferably comprises the following components in percentage by weight:

15-20% of sodium surfactin, 3.5-4% of poloxamer 184, 1.8-2.5% of cyclodextrin, 12-14% of PEG-400, 15-20% of polyalcohol, 0.3-1.0% of anti-wrinkle peptide compound, 0.3-0.4% of plant bacteriostatic agent, 1.5-3% of purslane extract and the balance of deionized water.

The polyhydric alcohol is one or more selected from 1, 3-propylene glycol, 1, 2-hexanediol, 1, 2-pentanediol, 1, 3-butanediol, and dipropylene glycol.

The plant bacteriostatic agent is one or more of Pleurotus Ministributus/radix Raphani fermentation product filtrate, lonicera Japonica flos extract, and Populus tremuloides bark extract.

The anti-wrinkle peptide compound is a mixture of one or two of pentapeptide-3, palmitoyl pentapeptide-4, tripeptide-1 copper, palmitoyl tetrapeptide-7, snake venom peptide and palmitoyl tripeptide-5 and resveratrol, wherein the resveratrol accounts for 15-25% of the weight of the anti-wrinkle peptide compound.

The pH of the stabilizing peptide of the present invention =5 to 6.

The preparation method of the stable peptide containing the active substances from fermentation sources comprises the following steps:

1. at normal temperature, deionized water, sodium surfactin, poloxamer 184, cyclodextrin and PEG-400 are stirred and mixed for 50-60 min.

2. And (2) after uniformly mixing the polyhydric alcohol and the anti-wrinkle peptide compound, slowly adding the mixture into the system in the step (1) under the stirring condition, and stirring at constant temperature and constant speed for 30-40 min to uniformly mix the materials.

3. And (3) uniformly mixing the plant bacteriostatic agent and the purslane extracting solution, adding the mixture into the system in the step (2), stirring at constant temperature and constant speed for 15-20 min, observing the uniformity of the material body, and inspecting to be qualified to discharge.

Compared with the prior art, the invention has the following beneficial effects:

the stable peptide not only can solve the stability factor of the peptide, but also has lower cost than freeze-dried powder, wherein the structure containing the active matter sodium surfactin from fermentation consists of a cyclic peptide hydrophilic group and a hydrophobic group, the cyclic peptide contains 7 amino acids, and the structure and the peptide have good compatibility and can stabilize the peptide component; the polyalcohol can preserve moisture, inhibit bacteria and promote seepage, and has strong auxiliary effect on the efficacy of peptides; the plant bacteriostatic agent is a pure plant extract component, has the effect of relieving the skin, and has small irritation and mild property. The product is transparent and colorless, has pH = 5-6, is close to the pH value of skin, has nanometer particle size, has the functions of promoting permeation and slow release, effectively exerts the efficacy of peptide raw materials, increases the transdermal absorption of the peptide raw materials, is used for a plurality of times at low concentration, is suitable for developing anti-aging repair products of sensitive muscles, and can be added into product systems of various formulations, including face cleaning, facial masks, smoothing toner, essence, emulsion, cream and the like.

Drawings

FIG. 1 shows the results of the stability test of the products of example 1 and comparative examples 1 to 2 at 45 ℃ for 30 days.

Detailed Description

The invention will be further described in detail with reference to the following figures and examples, but the scope of the invention is not limited to these examples.

Example 1

In this example, taking 100g of stable peptide containing fermentation-derived active ingredients as an example, the composition is: 25g of surfactin sodium, 184 g of poloxamer, 3g of cyclodextrin, 400 g of PEG (polyethylene glycol) -1 12g, 7.5g of 1, 3-propylene glycol, 7.5g of dipropylene glycol, 7.35g of palmitoyl tetrapeptide-7.35g, 0.5g of snake venom peptide, 0.15g of resveratrol, 0.2g of a fermentation product filtrate of deinococcus glutinosa/radish roots, 0.15g of lonicera japonica thunb flower extract, 0.15g of populus tremuloides bark extract, 4g of purslane extract and 35.5g of deionized water.

1. at normal temperature, 35.5g of deionized water, 25g of sodium surfactin, 4g of poloxamer 184, 3g of cyclodextrin and 12g of PEG-400 are stirred and mixed for 60min.

2. After 7.5g of 1, 3-propylene glycol, 7.5g of dipropylene glycol, 0.35g of palmitoyl tetrapeptide-7, 0.5g of snake venom peptide and 0.15g of resveratrol are uniformly mixed, the mixture is slowly added into the system in the step 1 under the stirring condition, and the mixture is stirred at constant temperature and constant speed for 30min to ensure that the materials are uniformly mixed.

3. And (3) uniformly mixing 0.15g of the lonicera sprangletop flower extract, 0.15g of the bark extract of populus tremuloides and 4g of the purslane extracting solution, adding the mixture into the system in the step (2), stirring the mixture at constant temperature for 15 to 20min at uniform speed, observing the uniformity of a material body, and discharging the mixture after the mixture is inspected to be qualified to obtain transparent and colorless stable peptide, wherein the pH of the stable peptide is =5.58.

Comparative example 1

In example 1, without adding sodium surfactin, the amount of deionized water was increased to 60.5g, and other raw materials and their amounts were the same as in example 1, and the resulting product had a pH =5.20.

Comparative example 2

In example 1, 8g of sodium surfactin was added, the amount of deionized water was increased to 52.5g, and other raw materials and their amounts were the same as in example 1, and the pH of the resulting product =5.40.

Comparative example 3

In example 1, palmitoyl tetrapeptide-7 and snake venom peptide were replaced with 0.03g of pentapeptide-3, the amount of resveratrol was reduced to 0.05g, the amount of deionized water was increased to 36.42g, the other raw materials and their amounts were the same as in example 1, and the resulting product had a pH =5.60.

Example 2

In this example, taking 100g of stable peptide containing fermentation-derived active ingredients as an example, the composition is: 25g of surfactin sodium, 184 g of poloxamer, 3g of cyclodextrin, 400 g of PEG-1, 2g of 1, 3-propylene glycol, 7.5g of dipropylene glycol, 4.2 g of palmitoyl pentapeptide, 0.65g of tripeptide-1 copper, 0.15g of resveratrol, 0.2g of a fermentation product filtrate of deinococcus mingiensis/radish roots, 0.15g of a lonicera japonica thunb extract, 0.15g of a bark extract of populus deltoides, 4g of a purslane extract and 35.5g of deionized water. The preparation method is the same as that of example 1, and the obtained product has a pH =5.60.

Example 3

In this example, taking 100g of stable peptide containing fermentation-derived active ingredients as an example, the composition is: 25g of surfactin sodium, 184 g of poloxamer, 3g of cyclodextrin, 400 g of PEG (polyethylene glycol) -1 12g, 7.5g of 1, 3-propylene glycol, 7.5g of dipropylene glycol, 3.75g of pentapeptide, 0.25g of resveratrol, 0.2g of a fermentation product filtrate of the deinococcus mingiensis/radish root, 0.15g of a lonicera japonica thunb flower extract, 0.15g of a bark extract of populus deltoides, 4g of a purslane extracting solution and 35.5g of deionized water. The preparation method is the same as that of example 1, and the pH of the obtained product is =5.62.

Example 4

In this example, taking 100g of stable peptide containing fermentation-derived active ingredients as an example, the composition is: 20g of surfactin sodium, 3.5g of poloxamer 184, 2.5g of cyclodextrin, 400 g of PEG-1, 4g of 1, 3-propylene glycol, 10g of 1, 2-pentanediol, 7.5g of 1, 2-pentanediol, 4.2g of palmitoyl pentapeptide, 0.55g of tripeptide-1 copper, 0.2g of resveratrol, 0.15g of a fermentation product filtrate of deinococcus micrantalis/radish root, 0.1g of a lonicera japonica thunb flower extract, 0.15g of a bark extract of populus tremuloides, 3g of a purslane extract and 38.15g of deionized water. The preparation method is the same as that of example 1.

Example 5

In this example, taking 100g of stable peptide containing fermentation-derived active ingredients as an example, the composition is: 15g of surfactin sodium, 184 g of poloxamer, 2g of cyclodextrin, 400 g of PEG, 10g of 1, 3-butanediol, 7.5g of dipropylene glycol, 3.75g of pentapeptide, 0.25g of resveratrol, 0.2g of a fermentation product filtrate of Leuconostoc/radish root, 0.2g of an extract of Lonicera sprawlata flower, 2g of a purslane extracting solution and 47.1g of deionized water. The preparation method is the same as that of example 1.

In order to prove the beneficial effects of the present invention, the inventors conducted a number of research experiments on the products of example 1 and comparative examples 1 to 3, the specific experiments being as follows:

1. stability test

The test method comprises the following steps: the products obtained in example 1 and comparative examples 1-2 are respectively filled into a 30mL experiment bottle, the bottle is placed in a constant temperature box for observation at 45 ℃, the appearance and the physical and chemical index changes of the sample are respectively observed for 1 day, 3 days, 7 days, 14 days and 30 days, the sample is kept at normal temperature compared with a standard sample, and the result of 30 days is shown in figure 1.

The results in FIG. 1 show the degree of yellowing: comparative example 1> comparative example 2> example 1, illustrating that the anti-wrinkle peptide of comparative example 1 is the least stable and the most stable in example 1, from which the active substance sodium surfactin, which is a fermentation source, is able to stabilize the anti-wrinkle peptide and protect it from discoloration.

2. Anti-aging efficacy test

Test subjects: 60 volunteers aged 20-60 years met the test conditions.

The use product is as follows: the product of example 1 is added into the essence to obtain a sample No. 1, the product of comparative example 1 is added into the essence to obtain a sample No. 2, the product of comparative example 2 is added into the essence to obtain a sample No. 3, and the product of comparative example 3 is added into the essence to obtain a sample No. 4, wherein the adding proportions of the products of example 1, comparative example 2 and comparative example 3 in the essence are the same.

The using method comprises the following steps: after cleaning the face in the morning and evening every day, taking a proper amount of test product, uniformly smearing the test product on the face, gently massaging the face until the test product is absorbed, smearing the No. 1 sample on 15 people, smearing the No. 2 sample on 15 people, smearing the No. 3 sample on 15 people, smearing the No. 4 sample on 15 people,

the evaluation method comprises the following steps: (1) anti-aging test: the wrinkle reduction effect was tested using a human skin rapid optical imaging system (PRIMOS, germany). After 10 minutes of cleaning, before applying the essence, after 30 minutes of use, after two weeks and four weeks of use, the results are shown in table 1, sa represents the average wrinkle depth. (2) repair test: the skin moisture loss is detected by adopting a German CK skin detector MPA580, after 10 minutes of cleaning, the evaluation is carried out before essence liquid is applied, the evaluation is carried out after 30 minutes of use, the evaluation is carried out after two weeks and four weeks of use, the result is shown in a table 2, TEWL represents the skin barrier repair level, and the more the data is reduced, the better the skin barrier repair effect is obtained.

TABLE 1 reduction of wrinkles Sa%

Sample(s)	Before use	After 30 minutes of use	Two weeks after use	After use, the four sides
					Sample No. 1	0	41.58	35.83	36.78
Sample No. 2	0	35.6	9.0	12.5
					Sample No. 3	0	38.5	20.2	15.0
Sample No. 4	0	41.23	5.8	5.0

TABLE 2 TEWL reduction%

And (4) evaluation results: the essence added into the product of the example 1 has obvious anti-aging effect and repairing effect.

3. Use of sensory tests

The test method comprises the following steps: screening 30 volunteers, cleaning face in the morning and evening every day, uniformly smearing a proper amount of test product on the face, gently massaging until the test product is absorbed, smearing No. 1 samples on 15 persons, and smearing No. 2 samples on 15 persons. Data collection was performed two weeks after use, four weeks after use.

The evaluation method comprises the following steps: the full score is 10 points, the very satisfactory score is 9 to 10 points, the satisfactory score is 7 to 8 points, the general score is 5 to 6 points, and the unsatisfactory score is 1 to 5 points (the satisfactory degree of improving the skin), and the results are shown in Table 3.

TABLE 3 satisfaction of Effect

Sample (I)	Two weeks after use	After use, the four sides are covered	Overall satisfaction (after use)
				Sample No. 1	8.3	8.7	8.6
Sample No. 2	6.5	6.7	6.9

And (4) evaluation results: as can be seen from the test results in Table 3, the essence added with the product of the example 1 has better skin feel and skin softness and tenderness, and the use sense is greatly influenced by not adding the sodium surfactin.

4. Patch test

The product of example 1 was tested using the closed patch test of the chapter seven skin patch test of cosmetic hygiene code 2015 edition, wherein 2 men and 28 women showed the test results in table 4.

TABLE 4

Note: the test substance was the product of example 1 (10% strength, dilution in deionized water) and the control in deionized water.

And (4) evaluation results: as can be seen from the results in Table 4, 0 of 30 patients showed positive reactions, indicating that the product of the present invention is safe to human body.

Claims

1. An active stabilizing peptide comprising a fermentation source, characterized by: the composition comprises the following components in percentage by weight: 10-25% of sodium surfactin, 3-5% of poloxamer 184, 1-3% of cyclodextrin, 10-15% of PEG-400, 11-22% of polyalcohol, 0.1-1.5% of anti-wrinkle peptide compound, 0.1-0.5% of plant bacteriostatic agent, 1-4% of purslane extract and the balance of deionized water;

the anti-wrinkle peptide compound is a mixture of palmitoyl tetrapeptide-7, snake venom peptide and resveratrol;

the resveratrol accounts for 15-25% of the mass of the anti-wrinkle peptide compound;

the plant bacteriostatic agent is one or more of Pleurotus Ministricosus/radix Raphani fermentation product filtrate, lonicera Japonica flos extract, and Populus tremuloides bark extract.

2. The fermentation-derived active stable peptide according to claim 1, wherein: the composition comprises the following components in percentage by weight: 15-20% of sodium surfactin, 3.5-4% of poloxamer 184, 1.8-2.5% of cyclodextrin, 12-14% of PEG-400, 15-20% of polyalcohol, 0.3-1.0% of anti-wrinkle peptide compound, 0.3-0.4% of plant bacteriostatic agent, 1.5-3% of purslane extract and the balance of deionized water.

3. The fermentation-derived active stable peptide according to claim 1 or 2, wherein: the polyalcohol is one or more selected from 1, 3-propylene glycol, 1, 2-hexanediol, 1, 2-pentanediol, 1, 3-butanediol, and dipropylene glycol.

4. The fermentation-derived active stable peptide according to claim 1 or 2, wherein: the pH of the stabilizing peptide = 5-6.