CN107802540B - Anti-aging composition and application thereof - Google Patents
Anti-aging composition and application thereof Download PDFInfo
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- CN107802540B CN107802540B CN201711235714.0A CN201711235714A CN107802540B CN 107802540 B CN107802540 B CN 107802540B CN 201711235714 A CN201711235714 A CN 201711235714A CN 107802540 B CN107802540 B CN 107802540B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
Abstract
The invention discloses an anti-aging composition, which comprises a polypeptide component and an anti-aging plant extract, wherein the anti-aging plant extract is at least one of alfalfa root nodule extract, artemisia leaf extract, sealwort extract and radish extract. The composition of the invention simultaneously comprises the polypeptide component and the anti-aging plant extract, and the polypeptide component and the anti-aging plant extract can play a role in synergy, thereby achieving a better anti-aging effect. Meanwhile, the invention also discloses application of the anti-aging composition in cosmetics.
Description
Technical Field
The invention relates to a composition, in particular to an anti-aging composition and application thereof, belonging to the field of cosmetics.
Background
The free radical theory of senescence states that degenerative changes in the senescence process are due to the deleterious effects of free radicals produced during normal cellular metabolism. The aging process of an organism is the result of accumulation of free radicals continuously produced by tissue cells of the organism, and the free radicals can cause DNA damage to cause mutation and induce tumor formation. Free radicals are intermediate products of normal metabolism, have strong reaction capability, can oxidize various substances in cells to damage biological membranes, and oxidative stress caused by excessive free radicals is an important cause of aging. As a result of skin aging, skin is relatively dry; the elasticity of the skin is poor, the skin is thinned, and wrinkles appear; the activity of sebaceous glands is reduced, and secretion is reduced; pigmented spots grow; the recovery of the injured skin becomes slow and the skin is likely to suffer from bruise.
Free radicals, also chemically referred to as "radicals", are radicals containing an unpaired electron. When atoms form molecules, electrons in chemical bonds must be paired, so that a free radical takes one electron of other substances to form a stable substance. In chemistry, this phenomenon is called "oxidation". Our biological systems are mainly exposed to oxygen radicals such as superoxide anion radicals, hydroxyl radicals, lipo-oxygen radicals, nitrogen dioxide and nitric oxide radicals, plus hydrogen peroxide, singlet oxygen and ozone, known as reactive oxygen, which account for over 95% of the free radicals in the body. Active oxygen radicals in the body have certain functions, such as immunity and signaling processes. However, excessive reactive oxygen radicals have destructive actions, resulting in damage to normal cells and tissues of the human body, thereby causing various diseases. In addition, sunlight radiation, air pollution, smoking, pesticides and the like in the external environment can generate more active oxygen free radicals to mutate nucleic acid, which is the root cause of human aging and diseases.
Disclosure of Invention
Based on this, the object of the present invention is to provide an anti-aging composition that addresses the above problems.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows: an anti-aging composition comprises a polypeptide component and an anti-aging plant extract, wherein the anti-aging plant extract is at least one of alfalfa root nodule extract, artemisia leaf extract, rhizoma polygonati extract and radish extract.
The composition of the invention simultaneously comprises the polypeptide component and the anti-aging plant extract, and the polypeptide component and the anti-aging plant extract can play a role in synergy, thereby achieving a better anti-aging effect.
Preferably, the weight part of the polypeptide component is 1.8-17 parts, and the weight part of the anti-aging plant extract is 4-40 parts.
Through a large amount of creative exploration, the inventor discovers that the polypeptide components and the anti-aging plant extract in parts by weight can further play a synergistic effect to achieve a better anti-aging effect.
Preferably, the polypeptide component is at least one of myristoyl pentapeptide-4, pentapeptide-1, acetyl dipeptide-1 cetyl ester and dipeptide diaminobutyryl benzyl amide diacetate.
More preferably, the polypeptide composition is a mixture of myristoyl pentapeptide-4, pentapeptide-1, acetyl dipeptide-1 cetyl ester, dipeptide diaminobutyrylbenzylamide diacetate.
Myristoyl pentapeptide-4: has outstanding effects of resisting aging, repairing damaged skin and stimulating collagen synthesis;
pentapeptide-1: the amino acid micromolecules can be directly absorbed and utilized by cells, and can promote skin collagen hyperplasia;
acetyl dipeptide-1 cetyl ester: the muscle contraction can be avoided, the generation of dynamic lines is reduced, the wrinkles are eliminated, the elasticity of collagen is effectively reorganized, the activity of elastin is enhanced, the lines of the face are relaxed, and the wrinkles are smoothed and the relaxation is improved;
dipeptide diaminobutyrylbenzylamide diacetate: is a synthetic peptide simulating snake venom serum, can prevent acetylcholine nerve conduction, achieves the effect of relaxing muscles, and thus improves dynamic wrinkles;
the four components act together, so that the synergistic interaction effect can be further exerted, and a better anti-aging effect is achieved.
More preferably, the weight ratio of the myristoyl pentapeptide-4, the pentapeptide-1, the acetyl dipeptide-1 cetyl ester to the dipeptide diaminobutyryl benzylamide diacetate is as follows: myristoyl pentapeptide-4: pentapeptide-1: acetyl dipeptide-1 cetyl ester: dipeptide diaminobutyrylbenzylamide diacetate (0.5-5): (0.5-5): (0.5-5): (0.3-2).
The four components in the weight ratio can further play a role in synergy, so that a better anti-aging effect is achieved.
Preferably, the anti-aging plant extract is a mixture of alfalfa root nodule extract, artemisia leaf extract, polygonatum rhizome extract and radish extract.
Root nodule extract of Medicago SATIVA (MEDICAGO SATIVA) has effects of promoting collagen production and resisting wrinkle; meanwhile, the water-absorbing and moisture-preserving fabric has water absorption and moisture-preserving effects.
The artemisia princeps (ARTEMISIA PRINCEPS) leaf extract has the effects of proliferating keratinocytes, enhancing skin cell metabolism, and activating skin and resisting aging; and can be used as antioxidant, skin whitening agent and antiinflammatory agent.
Rhizoma Polygonati (Polygonatum SIBIRICUM) extract has good collagen proliferation promoting effect, and can enhance metabolism of skin dermis, and has antioxidant effect, and can be used in cosmetics for activating skin and resisting wrinkle.
Radix Raphani (SCABIOSA ARVENSIS) extract has effects of effectively removing hydroxyl free radical and superoxide free radical, and resisting oxidation.
The four plant extracts can play a role in synergy, and achieve a good anti-aging effect.
More preferably, the weight ratio of the alfalfa root nodule extract, the artemisia leaf extract, the polygonatum rhizome extract and the radish extract is as follows: purple alfalfa root nodule extract: artemisia princeps leaf extract: and (3) extracting rhizoma polygonati: mountain radish extract ═ 1-10: (1-10): (1-10): (1-10).
The four components in the weight ratio can further play a role in synergy, so that a better anti-aging effect is achieved.
More preferably, the weight ratio of the alfalfa root nodule extract, the artemisia leaf extract, the polygonatum rhizome extract and the radish extract is as follows: purple alfalfa root nodule extract: artemisia princeps leaf extract: and (3) extracting rhizoma polygonati: mountain radish extract ═ 2-3: (3-5): (3-5): (4-6).
Meanwhile, the invention also provides a cosmetic containing the composition, and the cosmetic has a better anti-aging effect.
Preferably, the anti-aging composition accounts for 0.5 to 10 percent of the weight of the cosmetic.
Preferably, the cosmetic further comprises a cosmetic external preparation matrix.
Compared with the prior art, the invention has the beneficial effects that:
the composition of the invention simultaneously comprises the polypeptide component and the anti-aging plant extract, and the polypeptide component and the anti-aging plant extract can play a role in synergy, thereby achieving a better anti-aging effect. Meanwhile, the polypeptide components comprise myristoyl pentapeptide-4, pentapeptide-1, acetyl dipeptide-1 cetyl palmitate and dipeptide diaminobutyryl benzylamide diacetate, the plant extracts comprise alfalfa root nodule extract, artemisia leaf extract, rhizoma polygonati extract and radish extract, and the components can further exert a synergistic effect to achieve a more surprising anti-aging effect.
Detailed Description
To better illustrate the objects, aspects and advantages of the present invention, the present invention will be further described with reference to specific examples.
It should be noted that the process parameters, raw materials and the like which are not described in detail in the present invention are performed according to the conventional technical means in the art.
Example 1
In one embodiment of the antiaging composition of the invention, the antiaging composition of this embodiment comprises the following components in parts by weight:
polypeptide components (40.5 parts of myristoyl pentapeptide, 10.5 parts of pentapeptide, 0.5 part of acetyl dipeptide-1 cetyl ester and 0.3 part of dipeptide diaminobutyrylbenzyl amide diacetate) 1.8 parts of anti-aging plant extracts (1 part of alfalfa root nodule extract, 1 part of artemisia leaf extract, 1 part of rhizoma polygonati extract and 1 part of radish extract) 4 parts.
The cosmetic comprises the composition and a cosmetic external dosage form matrix, wherein the composition accounts for 0.5 percent of the weight of the cosmetic, the cosmetic external dosage form matrix can be selected by a person skilled in the art according to actual needs, and the cosmetic can be prepared according to a conventional method.
Example 2
In one embodiment of the antiaging composition of the invention, the antiaging composition of this embodiment comprises the following components in parts by weight:
17 parts of polypeptide components (myristoyl pentapeptide-45 parts, pentapeptide-15 parts, acetyl dipeptide-1 cetyl palmitate, 2 parts of dipeptide diaminobutyryl benzylamide diacetate), and 40 parts of anti-aging plant extracts (purple alfalfa root nodule extract 10 parts, Artemisia princeps. leaf extract 10 parts, rhizoma polygonati extract 10 parts, and radish extract 10 parts).
The cosmetic comprises the composition, wherein the mass percentage of the composition in the cosmetic is 2%, and the cosmetic external preparation type matrix can be selected by a person skilled in the art according to actual needs, and the cosmetic can be prepared according to a conventional method.
Example 3
In one embodiment of the antiaging composition of the invention, the antiaging composition of this embodiment comprises the following components in parts by weight:
10.5 parts of polypeptide components (42.5 parts of myristoyl pentapeptide, 13 parts of pentapeptide, 4 parts of acetyl dipeptide-1 cetyl palmitate and 1 part of dipeptide diaminobutyrylbenzylamide diacetate), and 22 parts of anti-aging plant extracts (5 parts of alfalfa root nodule extract, 4 parts of artemisia leaf extract, 6 parts of rhizoma polygonati extract and 7 parts of radish extract).
The cosmetic comprises the composition, wherein the content of the composition in the cosmetic is 4% by mass, and the cosmetic external preparation type matrix can be selected conventionally by a person skilled in the art according to actual needs, and the cosmetic can be prepared by a conventional method.
Example 4
In one embodiment of the antiaging composition of the invention, the antiaging composition of this embodiment comprises the following components in parts by weight:
10 parts of polypeptide components (myristoyl pentapeptide-44 parts, pentapeptide-13 parts, acetyl dipeptide-1 cetyl palmitate, 1 part of dipeptide diaminobutyryl benzyl amide diacetate), and 20 parts of anti-aging plant extracts (2 parts of alfalfa root nodule extract, 4 parts of artemisia leaf extract, 6 parts of rhizoma polygonati extract and 8 parts of radish extract).
The cosmetic comprises the composition, wherein the mass percentage of the composition in the cosmetic is 6%, and the cosmetic external preparation type matrix can be selected by a person skilled in the art according to actual needs, and the cosmetic can be prepared according to a conventional method.
Example 5
In one embodiment of the antiaging composition of the invention, the antiaging composition of this embodiment comprises the following components in parts by weight:
8 parts of polypeptide components (the weight ratio of myristoyl pentapeptide-4, pentapeptide-1, acetyl dipeptide-1 cetyl palmitate and dipeptide diaminobutyryl benzylamide diacetate is 0.5:1:5:2), and 30 parts of anti-aging plant extracts (the weight ratio of purple alfalfa root nodule extract, artemisia leaf extract, sealwort extract and radish extract is 2:3:3: 4).
The cosmetic comprises the composition, wherein the mass percentage of the composition in the cosmetic is 8%, and the cosmetic external preparation type matrix can be selected by a person skilled in the art according to actual needs, and the cosmetic can be prepared according to a conventional method.
Example 6
In one embodiment of the antiaging composition of the invention, the antiaging composition of this embodiment comprises the following components in parts by weight:
17 parts of polypeptide components (the weight ratio of myristoyl pentapeptide-4, pentapeptide-1, acetyl dipeptide-1 cetyl palmitate and dipeptide diaminobutyryl benzylamide diacetate is 5:1:3:0.3), and 4 parts of anti-aging plant extracts (the weight ratio of purple alfalfa root nodule extract, artemisia leaf extract, sealwort extract and radish extract is 3:5:5: 6).
The cosmetic comprises the composition, wherein the content of the composition in the cosmetic is 10% by mass, and the cosmetic external preparation type matrix can be selected conventionally by a person skilled in the art according to actual needs, and the cosmetic can be prepared by a conventional method.
Example 7
In one embodiment of the antiaging composition of the invention, the antiaging composition of this embodiment comprises the following components in parts by weight:
1.8 parts of polypeptide components (the weight ratio of myristoyl pentapeptide-4 to pentapeptide-1 to acetyl dipeptide-1 cetyl palmitate to dipeptide diaminobutyryl benzylamide diacetate is 1:1:5:1), and 40 parts of anti-aging plant extracts (the weight ratio of alfalfa root nodule extract, artemisia leaf extract, sealwort extract and radish extract is 2.5:4:4: 5).
The cosmetic comprises the composition, wherein the content of the composition in the cosmetic is 4% by mass, and the cosmetic external preparation type matrix can be selected conventionally by a person skilled in the art according to actual needs, and the cosmetic can be prepared by a conventional method.
Example 8
Hydroxy radical scavenging rate test
Control groups 1-3 are set, the control group 1 is the same as that in example 3 except that the control group 1 does not contain the polypeptide component, the control group 2 is the same as that in example 3 except that the control group does not contain the anti-aging plant extract, the control group 3 does not contain the polypeptide component and does not contain the anti-aging plant extract, and other external preparation type matrixes are the same as those in example 3.
Reagent: 1.865mmol/L o-diazaphenanthrene in absolute ethanol, 0.2M pH 7.4 phosphate buffer, 1.865mmol/L FeSO 4.7H 2O solution, 0.03% (v/v) H2O2Distilled water and 0.8mg/ml solution to be detected.
The method comprises the following steps: taking 1.0mL of absolute ethanol solution of 1.865mmol/L o-diazaphenanthrene in a test tube with a plug, respectively adding 2mL of pH 7.4 phosphate buffer solution with the concentration of 0.2mol/L and 1mL of to-be-detected solution, fully and uniformly mixing, adding 1.0mL of FeSO with the concentration of 1.865mmol/L4·7H2O solution, mixed again and then added with 1.0mL of 0.03% (v/v) H2O2Measuring absorbance values As of each group of mixed solution respectively at 37 ℃ in constant-temperature water bath for 60min under 536nm, measuring absorbance values Ab by using distilled water instead of a sample As a blank group, and replacing H by distilled water2O2As the lesion group, the absorbance value An was measured, and the hydroxyl radical clearance of the antioxidant was calculated according to the following formula: hydroxyl radical clearance (%) [ (As-An)/(Ab-An)]X 100, the average of three measurements was taken, and the results are shown in table 1.
DPPH radical scavenging Rate experiment
Reagent: 0.0178mmol/L DPPH-absolute methanol solution, absolute ethyl alcohol and 0.8mg/ml solution to be detected.
The method comprises the following steps: taking 3mL of 0.0178mmol/L DPPH-anhydrous methanol solution, adding 3mL of solution to be detected into a cuvette, uniformly mixing, immediately measuring the light absorption value at the wavelength of 517nm, marking the light absorption value as Ai, then measuring the light absorption value after storing in dark at 37 ℃ for 30min, marking the light absorption value as Aj, and marking the light absorption value as Ac if only the DPPH-anhydrous methanol solution is added as a blank control. DPPH radical clearance was calculated as follows: DPPH radical clearance (%) [1- (Ai-Aj)/Ac ] × 100%, the average of three measurements was taken, and the results of the measurements are shown in table 1.
Table 1 free radical scavenging test
As can be seen from table 1, compared with comparative example 3 (containing no plant extract and polypeptide component of the present invention), examples 1 to 7 (containing plant extract and polypeptide component), comparative example 1 (containing only plant extract), and comparative example 2 (containing only polypeptide component) have significantly higher radical scavenging rate than comparative example 3, and have significantly better anti-aging effect, which indicates that both plant extract and polypeptide component in the cosmetic of the present invention have excellent radical scavenging performance. Meanwhile, compared with comparative example 1 and comparative example 2, the skin care products of examples 1-7 with the plant extracts and the polypeptide components added simultaneously have the radical scavenging rate higher than the sum of the radical scavenging rates of the comparative example 1 and the comparative example 2, which shows that the plant extracts and the polypeptide components have synergistic effect and have better anti-aging efficacy.
Example 9
(1) Skin roughness test:
pretreatment in testing: the test population was 40 wrinkled and healthy skin lesions aged 35-45 years, randomly divided into 4 groups of 10, and prepared into cosmetics using the compositions of examples 1-7. The subjects applied the test samples to the face twice a day, morning and evening. During the experiment, the subject was not able to apply any other cosmetic product at the experimental site. The test subjects performed the test after 0, 1, 2, 3, 4 weeks of continuous use of the test samples.
Skin roughness test: the skin condition was measured at week 0, week 1, week 2, week 3 and week 4 using a wrinkle tester VISION SV600, and the test site was the cheekbone. The test parameters were skin roughness R1 (the difference between the highest peak and the lowest valley of the dermatoglyph), mean change R1 from the 4 th week R1 to the 0 th week R1, mean change R1 from R1/week 0R 1. Average roughness R3 (arithmetic mean of 5 consecutive segments of the same length), average change in R2 from R3 at cycle 4 to R3 at cycle 0, and average change in R3 from R3/R3 at cycle 0. The higher the values of R1 and R3, the more severe the wrinkles, and the test results for specific R1 and R3 are shown in table 2 and table 3, respectively:
TABLE 2 Change in skin roughness R1 after cosmetic preparation Using the compositions of examples 1 to 7
| Group of | Mean change value (mm) | Average rate of change (%) |
| Example 1 | -0.73 | -39.74 |
| Example 2 | -0.78 | -41.56 |
| Example 3 | -0.80 | -43.92 |
| Example 4 | -0.84 | -46.00 |
| Example 5 | -0.87 | -47.89 |
| Example 6 | -0.91 | -48.24 |
| Example 7 | -0.99 | -48.97 |
TABLE 3 variation in average roughness of skin R3 after cosmetic preparation Using the compositions of examples 1 to 7
The experimental results in table 2 show that the cosmetic prepared by using the compositions in examples 1 to 7 can significantly reduce the difference between the highest peak and the lowest valley of the skin lines, i.e., significantly reduce the roughness of the skin, and the roughness of the skin can be reduced by more than 39.74% after the product is used for four weeks.
From the experimental results in Table 3, it can be seen that the average skin roughness of the cosmetics prepared using the compositions of examples 1 to 7 was reduced by 40.97% or more for four weeks. As can be seen from the results of tables 2 and 3, wrinkles can be effectively reduced using the cosmetics of the present invention.
(2) Skin elasticity test:
pretreatment in testing: the test population was 40 wrinkled and healthy skin lesions aged 35-45 years, randomly divided into 4 groups of 10, and prepared into cosmetics using the compositions of examples 1-7. The subjects applied the test samples to the face twice a day, morning and evening. During the experiment, the subject was not able to apply any other cosmetic product at the experimental site. The test subjects performed the test after 0, 1, 2, 3, 4 weeks of continuous use of the test samples.
Skin elasticity test: the skin condition at 0 week, 1 week, 2 weeks, 3 weeks and 4 weeks was measured using the skin elasticity tester MPA580, the test site was the cheekbone part, the probe repeated 3 times in the same test area during the measurement, and the average value was taken. The test parameter is R2 (the ratio of the springback amount Ua of the skin without negative pressure to the maximum stretch amount Uf with negative pressure), the closer R2 is to 1, the better the elasticity of the skin, the average change value of R2 is the 4 th cycle R2 value to the 0 th cycle R2 value, and the average change rate of R2 is the average change value of R2/the 0 th cycle R2 value. The results of the specific skin elasticity tests are shown in table 4:
TABLE 4 Change in skin elasticity R2 after preparation of the resulting cosmetic products Using the compositions of examples 1 to 7
As can be seen from the data in Table 4, the cosmetics prepared by using the compositions in examples 1-7 can effectively improve the elasticity of skin, which shows that the product of the invention can effectively resist aging.
Example 10
The test is divided into a control group and an experimental group, wherein the control group is a matrix only containing a cosmetic external preparation form, the experimental group comprises 21 groups, the experimental group 1 to 7 are respectively cosmetics prepared by the composition in the example 1 to 7, the components contained in the cosmetics in the experimental group 8 to 21 are the same as the components in the example 3 except for the content of the four components of the myristoyl pentapeptide-4, the pentapeptide-1, the acetyl dipeptide-1 cetyl palmitate and the dipeptide diaminobutyrylbenzylamide diacetate (the total content of the four components in the cosmetics is the same, but the weight ratio is different), the weight ratio of the four components of the myristoyl pentapeptide-4, the pentapeptide-1, the acetyl dipeptide-1 cetyl palmitate and the dipeptide diaminobutyrylbenzylamide diacetate in the specific experimental group 8 to 21 is shown in the following table 5:
TABLE 5 weight ratio of myristoyl pentapeptide-4, pentapeptide-1, acetyl dipeptide-1 cetyl ester, dipeptide diaminobutyryl benzylamide diacetate in experimental group 8-21 cosmetic
In the control group and the experimental group, 10 women of 35 to 45 years old who had wrinkles and had no damage to skin health were randomly selected as test subjects for each group, cosmetics containing only a cosmetic base material for external use were used for the test subjects of the control group, cosmetics containing the compositions described in examples 1 to 7 were used for the test subjects of the experimental group 1 to 7, and cosmetics prepared in the experimental groups 8 to 21 were used for the test subjects of the experimental group 8 to 21, and the change in skin average roughness before and after use was measured by the same application method and skin roughness measurement method as in example 9, and the results are shown in table 6.
TABLE 6 variation of skin roughness average R3
As can be seen from Table 6, the cosmetics of the experimental groups 1-21 all had better anti-aging effects than the cosmetics of the control group. The cosmetics of experimental groups 1-7 contain four components of myristoyl pentapeptide-4, pentapeptide-1, acetyl dipeptide-1 cetyl ester and dipeptide diaminobutyryl benzylamide diacetate, and the effect is best. The cosmetics of the experimental groups 8-21 only contain one, two or three of the four components of myristoyl pentapeptide-4, pentapeptide-1, acetyl dipeptide-1 cetyl palmitate and dipeptide diaminobutyrylbenzylamide diacetate, and the anti-aging effect is slightly weak, which shows that the four components of myristoyl pentapeptide-4, pentapeptide-1, acetyl dipeptide-1 cetyl palmitate and dipeptide diaminobutyrylbenzylamide diacetate have the effects of mutual promotion and mutual synergy and can play a remarkable anti-aging effect.
Example 11
The test is divided into a control group and an experimental group, the control group only contains a cosmetic external preparation form matrix, the experimental group comprises 21 groups, wherein the experimental group 1-7 respectively are the cosmetics prepared by the composition in the embodiment 1-7, the components contained in the cosmetics in the experimental group 8-21 are the same as the components in the embodiment 3 except that the contents of four components including the alfalfa root nodule extract, the artemisia leaf extract, the polygonatum rhizome extract and the carrot extract are different (the total contents of the four components in the cosmetics are the same, but the weight ratios are different), the weight ratios of the four components including the alfalfa root nodule extract, the artemisia leaf extract, the polygonatum rhizome extract and the carrot extract in the specific control group 1-14 are shown in table 7:
table 7 weight ratios of four components of alfalfa root nodule extract, artemisia leaf extract, sealwort extract and radish extract in experimental group 8-21 cosmetics
| Group of | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 |
| Root nodule extract of alfalfa | 5 | 0 | 0 | 0 | 5 | 5 | 5 | 0 | 0 | 0 | 5 | 5 | 0 | 5 |
| Artemisia princeps leaf extract | 0 | 4 | 0 | 0 | 4 | 0 | 0 | 4 | 4 | 0 | 4 | 4 | 4 | 0 |
| Polygonatum sibiricum extract | 0 | 0 | 6 | 0 | 0 | 6 | 0 | 6 | 0 | 6 | 6 | 0 | 6 | 6 |
| Extract of mountain radish | 0 | 0 | 0 | 7 | 0 | 0 | 7 | 0 | 7 | 7 | 0 | 7 | 7 | 7 |
In a control group and an experimental group, 10 women of 35-45 years old who have wrinkles and do not have skin health damage are randomly selected as test objects in each group, the test objects in the control group use cosmetics only containing cosmetic external-use formulation matrixes, the test objects in the experimental groups 1-7 use cosmetics containing the composition in examples 1-7, the test objects in the experimental groups 8-21 use cosmetics prepared in the experimental groups 8-21, the change conditions of the average roughness of the skin before and after use are tested by the same using method and the skin roughness testing method as those in example 9, and the results are shown in table 8.
TABLE 8 variation of skin roughness average R3
As can be seen from Table 8, the cosmetics of the experimental groups 1 to 21 all had better anti-aging effects than the cosmetics of the control group. The cosmetics of the experimental group 1-7 contain four components of alfalfa root nodule extract, artemisia leaf extract, sealwort extract and radish extract, and have good anti-aging effect. The cosmetics in the experimental group 8-21 only contain one, two or three of the four components of the purple alfalfa root nodule extract, the artemisia leaf extract, the sealwort extract and the carrot extract, and the anti-aging effect is weak, so that the four components of the purple alfalfa root nodule extract, the artemisia leaf extract, the sealwort extract and the carrot extract have the mutual promotion and mutual synergy effects, and can play a remarkable anti-aging effect.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the protection scope of the present invention, and although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.
Claims (4)
1. An anti-aging composition is characterized by comprising 1.8-17 parts by weight of a polypeptide component and 4-40 parts by weight of an anti-aging plant extract; the polypeptide components are a mixture of myristoyl pentapeptide-4, pentapeptide-1, acetyl dipeptide-1 cetyl ester and dipeptide diaminobutyryl benzylamide diacetate, and the weight ratio of myristoyl pentapeptide-4, pentapeptide-1, acetyl dipeptide-1 cetyl ester to dipeptide diaminobutyryl benzylamide diacetate is as follows: myristoyl pentapeptide-4: pentapeptide-1: acetyl dipeptide-1 cetyl ester: dipeptide diaminobutyrylbenzylamide diacetate (0.5-5): (0.5-5): (0.5-5): (0.3-2); the anti-aging plant extract is a mixture of a purple alfalfa root nodule extract, a artemisia leaf extract, a rhizoma polygonati extract and a radish extract; the weight ratio of the alfalfa root nodule extract, the artemisia leaf extract, the sealwort extract and the radish extract is as follows: purple alfalfa root nodule extract: artemisia princeps leaf extract: and (3) extracting rhizoma polygonati: mountain radish extract ═ 1-10: (1-10): (1-10): (1-10).
2. The anti-aging composition according to claim 1, wherein the alfalfa root nodule extract, artemisia inflata extract, polygonatum sibiricum extract and radish spicebush extract are in a weight ratio of: purple alfalfa root nodule extract: artemisia princeps leaf extract: and (3) extracting rhizoma polygonati: mountain radish extract ═ 2-3: (3-5): (3-5): (4-6).
3. A cosmetic comprising the composition according to any one of claims 1 to 2.
4. The cosmetic of claim 3, wherein the anti-aging composition is present in the cosmetic in an amount of 0.5 to 10% by weight.
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| CN201711235714.0A CN107802540B (en) | 2017-11-29 | 2017-11-29 | Anti-aging composition and application thereof |
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| CN110269832A (en) * | 2018-10-25 | 2019-09-24 | 广州暨大美塑生物科技有限公司 | A kind of anti-aging Essence of the fungus extract containing root nodule and preparation method thereof |
| CN109527587A (en) * | 2019-01-03 | 2019-03-29 | 拓普河北生物科技有限公司 | A kind of collagen Gly-His-Lys and preparation method thereof with senile-resistant efficacy |
| KR102502510B1 (en) * | 2019-10-18 | 2023-02-23 | 동아제약 주식회사 | Compositions for improving skin damage comprising Artemisia princeps leaf extract |
| CN112675060B (en) * | 2021-01-06 | 2022-08-26 | 山东华熙海御生物医药有限公司 | Composition and skin care product for resisting skin aging and preparation method thereof |
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| CN104888183A (en) * | 2015-06-16 | 2015-09-09 | 郑相国 | Health-preserving health-care Chinese patent medicine |
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