KR20110012699A - Cosmetic composition containing cyclic lipopeptide for antioxidant and antiaging - Google Patents

Abstract

PURPOSE: A cosmetic composition containing cyclic lipopeptide is provided to suppress MMP-1 expression and to treat skin wrinkling. CONSTITUTION: A cosmetic composition contains 0.0001-30 weight% of iturin A or surfactin as an active ingredient. The iturin A or surfactin is a cyclic lipopeptide isolated from Bacillus sp. The iturin A or surfactin is used in health food additive and drug composition.

Description

Cosmetic composition containing cyclic lipopeptide for antioxidant and antiaging

The present invention relates to a cosmetic composition containing cyclic lipopeptides, and more particularly, iturin A and surfactin, which are cyclic lipopeptides (Cyclicl ipopeptide) obtained from Bacillus sp. It relates to a cosmetic composition having antioxidant and anti-aging effects by containing (Surfactin).

Human skin is changed by a number of internal and external factors as it ages. That is, internally, the secretion of various hormones that regulate metabolism decreases, and the function of immune cells and the activity of cells decreases, thereby reducing the biosynthesis of immune proteins and constituent proteins necessary for living organisms. Due to the increase in the amount of ultraviolet rays that reach the surface of the sun's rays and further increase environmental pollution, free radicals and free radicals, such as free radicals, skin thickness is reduced, wrinkles are increased, elasticity is reduced only In addition, the color of the skin becomes dull, skin problems frequently occur, and blemishes, freckles, and mushrooms are also increasing.

As aging progresses, symptoms such as changes and decreases in the content and arrangement of collagen, elastin, hyaluronic acid, and glycoproteins, which are constituting skin, appear, and are subjected to oxidative stress caused by free radicals and active harmful oxygen. In addition, cyclooxygenase-2 (Cox-2, cyclooxygenase), an enzyme that produces proinflammatory cytokine, which is known to cause inflammation, in most cells constituting the skin due to aging or ultraviolet rays. Biosynthesis increases, and the biosynthesis of matrix metalloproteinase (MMP), an enzyme that breaks down skin tissues by these inflammatory factors, increases NO (nitric oxide) production by inducible nitric oxide synthase (iNOS). It is known to increase. In other words, the biosynthesis of the substrate material is reduced by the reduction of cellular activity and micro-inflammation due to naturally occurring endogenous aging, and by external factors such as the increase of stress caused by various harmful environments and the increase of free radical species caused by sunlight. As a result, decomposition and degeneration are accelerated, and the skin matrix is destroyed and thinned, resulting in various symptoms of skin aging. Therefore, a lot of research is being conducted on the active ingredient that can prevent and improve the phenomenon of aging.

Therefore, the present inventors were studying the skin related effects of cyclic lipopeptides, iturin A and surfactin, extracted from Bacillus sp., And found that these cyclolipopeptides have excellent skin antioxidant and anti-aging effects. To complete.

Accordingly, an object of the present invention is to provide a cosmetic composition having antioxidant and anti-aging effects by containing cyclic lipopeptides obtained by extraction from Bacillus sp.

In order to achieve the above object, the present invention provides a cosmetic composition containing at least one selected from iturin A and surftin as an active ingredient.

The cosmetic composition according to the present invention exhibits antioxidant effect and MMP-1 expression inhibition effect by containing cyclic lipopeptides, iturin A and surfactin, which are extracted from Bacillus sp. Synergism can provide excellent skin wrinkle improvement.

The cosmetic composition provided by the present invention contains at least one of cyclic lipopeptides, iturin A and surfactin, as an active ingredient. The active ingredient is contained in an amount of 0.0001 to 30% by weight based on the total weight of the composition. If the content is less than 0.0001% by weight, it is not possible to obtain antioxidant and anti-aging effects according to the use of the above components, and if the content is more than 30% by weight, the increase in effect is not large.

Iturin A used in the present invention belongs to the group of iturin antibiotics. Usually, iturin antibiotics are antifungal antibiotics produced from the genus Bacillus sp. As a result, 7 α-amino acids and 1 β-amino acid are used. It has a combined annular structure. In addition, β-amino acids vary in the number of carbon atoms according to the number of methylenes and have the same molecular weight, even though the same antibiotics have been reported to increase the antifungal activity as the carbon number increases. Among them, iturin A has 7 amino acids (3 Asn, 1 Gln, Pro, Ser, Tyr) and various number of characteristic hydrophobic fatty acid chains (Ser-β-amino acids). Therefore, the molecular weight is present in a variety of ranges from 1,043 to 1,085.

Surfactin used in the present invention also has the ability to have a strong surfactant and is generally used as an antibiotic. The structure generally has 7 to 15 amino acids (L-asparagine, L-leucine, glycine, L-leucine, L-valine and two D-leucine) and 13 to 15 hydrophobic fatty acid chains.

The cosmetic composition containing the iturin A and surfactin of the present invention has an antioxidant effect and MMP-1 expression inhibitory effect, it can be expected excellent skin wrinkle improvement effect by the combined synergistic action of the two activities.

Iturin A and surfactin used in the present invention are not particularly limited in its use, and can be used, for example, in addition to cosmetic compositions, additives of health foods, pharmaceutical compositions and the like.

Hereinafter, the present invention will be described in more detail with reference to Examples and Test Examples, but the present invention is not limited only to these examples.

Test Example 1 Antioxidant Effect Test (DCF-da)

HaCaT cells were placed in a 96-hole plate at 1.5 X 10 ⁴ cells / well, and after 24 h, iturin A (YM Chemical) and Surfactin (YM Chemical) respectively 50 ppm was dissolved in DMEM medium and treated. At this time, 100 μM or 200 μM of vitamin C was used as a positive control, and nothing was used as a negative control. At this time, the treatment time was about 3 hours and then washed with HBSS. Thereafter, DCF 50 μM (in HBSS) was treated with the cells and allowed to react for 20 minutes while blocking light at 37 ° C. After washing twice with HBSS and 100 μL of HBSS was measured fluorescence (485nm / 535nm, 0.1s) was measured. The samples were then treated with UVB at 30 mJ for 80 seconds and measured again after 2 hours to compare with initial values. The experimental results are shown in FIG. 1.

1, it can be seen that when treated with iturin A or surftin, the ratio of the fluorescence value after UV treatment appeared to a degree similar to that of vitamin C, a known antioxidant.

Thus, it can be seen that iturin A and surfactin can provide excellent antioxidant effects.

Test Example 2 Measurement of Collagenase Expression Inhibition Efficacy

The inhibitory activity of collagenase expression of iturin A and surfactin was measured in comparison with retinoic acid. The lower the expression level of collagenase, the higher the expression inhibiting ability of collagenase, less breakdown of collagen in the skin, and therefore, the smaller the amount of wrinkles produced. In addition, retinoic acid is an antioxidant that is known to have a function of preventing skin aging by regenerating epidermal cells of the skin.

The test was performed by placing human fibroblasts at 5,000 cells / well in a 96-well microtiter plate containing Dulbecco's Modified Eagle's Media (DMEM) medium containing 2.5% fetal calf serum. Incubate until 90% growth. After 24 hours of incubation in serum-free DMEM medium, the iturin A (YM Chemical) and Surfactin (YM Chemical) were dissolved in serum-free DMEM medium. After treatment with retinoic acid at a concentration of 10 μM for 24 hours, the cell culture was collected.

The collected cell culture medium was measured for collagenase production using a commercially available collagenase measuring instrument (Amersham Pharma Co., Ltd., USA). First, the collected cell culture was placed in a 96-well plate uniformly coated with primary collagenase antibody, and the antigen-antibody reaction was performed in a thermostat for 3 hours.

After 3 hours, the chromophore-bound secondary collagen antibody was placed in a 96-well plate and reacted for another 15 minutes. After 15 minutes, the coloring stimulant was added, causing color development at room temperature for 15 minutes, and 1M sulfuric acid was added again to stop the reaction (color development). The color of the reaction solution was yellow and the degree of yellow color was different according to the progress of the reaction.

The absorbance of the yellowish 96-well plate was measured at 405 nm using an absorbance meter, and the degree of synthesis of collagenase was calculated by Equation 1 below. At this time, the reaction absorbance of the cell culture liquid collected from the group not treated with the composition was used as a control. That is, the expression level of collagenase in the untreated group was 100, and the expression level of collagenase in the group treated with the composition was calculated, and the results are shown in Table 1 and FIG. 2.

matter Collagenase
Expression degree (%) UVB no treatment group 100 UVB 40mJ Probe 152 Retinoic acid 83 Iturin A 89 Surfactin 120

In Table 1, iturin A and surfactin according to the present invention can be confirmed that significantly inhibit the expression of collagenase in vitro ( in vitro ) compared to the negative control group of UVB irradiated group and The case can be seen to show a high MMP-1 expression inhibitory effect similar to the retinoic acid positive control.

Although the formulation example of the composition containing the iturin A or surfactin of this invention is demonstrated below, the cosmetic composition of this invention is not limited only to these examples.

Formulation Example 1 Nutritional Cosmetics

Nourishing lotion containing iturin A or surfactin was prepared with the composition shown in Table 2 below.

ingredient Content (% by weight) At least one of iturin A and surfactin 5.0 Squalane 5.0 Beeswax 4.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.5 Liquid paraffin 0.5 Caprylic / capric triglyceride 5.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxy Vinyl Polymer 0.1 Triethanolamine 0.2 Preservative, coloring, fragrance Quantity Purified water Balance Sum 100

Formulation Example 2 Flexible Cosmetics

A flexible lotion containing iturin A or surfactin was prepared with the composition shown in Table 3 below.

ingredient Content (% by weight) At least one of iturin A and surfactin 5.0 glycerin 3.0 Butylene glycol 2.0 Propylene glycol 2.0 Carboxy Vinyl Polymer 0.1 PEG 12 nonylphenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 Triethanolamine 0.1 Preservative, coloring, fragrance Quantity Purified water Balance Sum 100

Formulation Example 3 Nutrition Cream

Nutritional creams containing iturin A or surfactin were prepared in the compositions shown in Table 4 below.

ingredient Content (% by weight) At least one of iturin A and surfactin 5.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.5 PEG60 Cured Castor Oil 2.0 Liquid paraffin 10.0 Squalane 5.0 Caprylic / Caprol Triglycerides 5.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservative, coloring, fragrance Quantity Purified water Balance Sum 100

Formulation Example 4 Massage Cream

Massage creams containing iturin A or surfactin were prepared in the compositions shown in Table 5 below.

ingredient Content (% by weight) At least one of iturin A and surfactin 5.0 Beeswax 10.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.8 PEG60 Cured Castor Oil 2.0 Liquid paraffin 40.0 Squalane 5.0 Caprylic / Caprol Triglycerides 4.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservative, coloring, fragrance Quantity Purified water Balance Sum 100

[Formulation Example 5] Pack

Packs containing iturin A or surfactin were prepared with the compositions shown in Table 6 below.

ingredient Content (% by weight) At least one of iturin A and surfactin 5.0 Polyvinyl alcohol 13.0 Sodium Carboxymethyl Cellulose 0.2 glycerin 5.0 Allantoin 0.1 ethanol 6.0 PEG 12 nonylphenyl ether 0.3 Polysorbate 60 0.3 Preservative, coloring, fragrance Quantity Purified water Balance Sum 100

Figure 1 shows the results of DCF da experiments when HaCaT cells were treated with iturin A, surfactin and vitamin C.

Figure 2 shows the results measured by comparing the collagenase expression inhibitory ability of iturin A and surfactin with retinoic acid.

Claims (4)

Cosmetic composition containing at least one selected from iturin A and surfactin as an active ingredient. The cosmetic composition according to claim 1, wherein the active ingredient is contained in an amount of 0.0001 to 30% by weight based on the total weight of the composition. According to claim 1, wherein the composition is a cosmetic composition, characterized in that for antioxidant. According to claim 1, wherein the composition is a cosmetic composition, characterized in that for anti-aging.

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