CN112891270B

CN112891270B - Cosmetic composition and preparation method and application thereof

Info

Publication number: CN112891270B
Application number: CN202110135050.0A
Authority: CN
Inventors: 洪怡
Original assignee: Wuhan Runhe Biological Medicine Co ltd
Current assignee: Wuhan Runhe Biological Medicine Co ltd
Priority date: 2021-01-29
Filing date: 2021-01-29
Publication date: 2023-02-07
Anticipated expiration: 2041-01-29
Also published as: CN112891270A

Abstract

The invention discloses a cosmetic composition liposome gel for skin care, which has the functions of maintaining beauty and keeping young, whitening and protecting skin, delaying senility, maintaining beauty and removing freckles. The formula is prepared by adopting a unique formula and a unique proportion and a unique preparation process, and the product has the advantages of regular morphological structure, moderate size, good dispersibility, uniform particle size distribution, high encapsulation efficiency, good stability, good curative effect and safe and convenient application.

Description

Cosmetic composition and preparation method and application thereof

Technical Field

The invention belongs to the technical field of cosmetics, and relates to a cosmetic composition, and a preparation method and application thereof.

Background

In recent years, with the improvement of living standard of people, more and more people use cosmetics such as skin cream, skin lotion, skin gel and the like, however, the existing cosmetics have the defect of poor whitening effect, cannot achieve the purposes of beautifying, whitening and protecting skin and delaying aging on skin at the same time, and are difficult to meet the requirements of people. In addition, different users have different skin types, and different skin types are adapted to cosmetics of different compositions. The existing cosmetics have fixed compositions and are difficult to meet the requirements of various skins. In addition, cosmetic allergy even accidents that cause serious damage to the health of consumers occur occasionally, and consumers are calling for higher and higher safety appeal of cosmetics. The reason for this is that apart from the fact that some illegal merchants use forbidden raw materials, the influence of the long-term use of the artificially synthesized raw materials which can be used legally now on the health of consumers is not negligible. For example, the paraben preservatives, which are used in cosmetics in large quantities, have been studied in recent years to have potential estrogenic activity, which can cause reduced sperm motility and lead to sterility reproductive toxicity. Correspondingly, the raw materials derived from plants have the characteristics of reproducibility, small stimulation, high safety and remarkable effect.

Therefore, cosmetics with better safety for maintaining beauty and keeping young, whitening and protecting skin, delaying aging, maintaining beauty and removing freckles still need to be further researched.

Disclosure of Invention

The present invention aims to solve at least one of the above technical problems to at least some extent or to at least provide a useful commercial choice. Therefore, the invention aims to provide a cosmetic composition which simultaneously aims at beautifying, whitening and protecting skin, delaying senility and removing freckles and has better safety. The prescription is prepared by adopting a unique formula, a unique proportion and a unique preparation process, and has good curative effect, safety and convenience.

Thus, in one aspect of the present invention, the present invention provides a cosmetic composition comprising: grape seed extract, white peony root extract, crabapple oil, vitamin C ethyl ether, and vitamin E.

According to an embodiment of the present invention, the cosmetic composition comprises, in parts by weight: 0.2 to 1 weight portion of grape seed extract, 0.2 to 1 weight portion of white peony root extract, 0.1 to 0.3 weight portion of Indian crabapple oil, 0.15 to 1.5 weight portions of vitamin C ethyl ether and 0.05 to 0.5 weight portion of vitamin E.

Based on the previous series of researches, the inventor carries out a great deal of beneficial exploration on the combined potential of substances for beautifying, whitening and protecting skin and delaying senility and obtains unexpected effects. For this reason, the inventor goes through hard creative work and optimization work, and surprisingly discovers that: the composition prepared from the raw materials of grape seed extract, white peony root extract, indian fruit oil, vitamin C ethyl ether, vitamin E and the like has obvious effects on maintaining beauty, keeping young, whitening and protecting skin, delaying aging and the like. In addition, the applicant of the present invention also unexpectedly found through experiments that: the composition also has effects of caring skin and removing speckle. And the inventors of the present invention found that the composition has a significant synergistic effect. The formula of the invention is further used for preparing skin cream, skin lotion, liposome gel for skin care and the like containing grape seed extract, white peony root extract, indian fruit oil, vitamin C ethyl ether, vitamin E and the like.

According to the embodiment of the invention, the grape seed extract is an effective active nutrient component extracted from natural grape seeds, and the main extract procyanidin (OPC) of the grape seeds has the effects of whitening and removing freckles, is a substance which is found in nature and has the strongest capacity of resisting oxidation and removing free radicals, has the antioxidant activity which is 50 times that of vitamin E and 20 times that of vitamin C, can effectively remove redundant free radicals in a human body, has the super-strong functions of delaying senescence and enhancing immunity, and is used for resisting oxidation, resisting allergy, resisting fatigue, enhancing physique, improving sub-health state, delaying senescence, improving dysphoria, irritability, dizziness, hypodynamia, hypomnesia and the like.

The white peony root extract is a natural extract which is extracted from Paeonia lactiflora (Paeonia lactiflora) of Ranunculaceae, refined, concentrated and dried, and has the main component of paeoniflorin, which is easily soluble in methanol and ethanol, stable in acidity and instability in alkalinity, and good in thermal stability, has the effects of resisting inflammation, easing pain, hypnosis, resisting convulsion and regulating immunity, and can be widely used in medicines, health products and cosmetics.

The crabapple oil contains various polyunsaturated fatty acids, tocopherol and polyphenol compounds, and the compounds have good oxidation resistance and free radical scavenging capacity, prevent the oxidation of skin and peroxidation caused by free radicals to a certain extent, achieve the effects of maintaining beauty, keeping young, resisting wrinkles and resisting aging, are widely applied to the cosmetic industries such as face cleansing cream, face cream, eye cream, bath cream, shampoo and the like, have high nonirritant and safety, and are good cosmetic raw materials.

Vitamin C can inhibit melanin generation and enhance collagen synthesis to delay aging and whiten skin (references: sugui chess, huanghe and Lin, jianna, etc.. Action of vitamin C and common adverse reactions [ J ]. Latest medical information abstracts in the world, 2019,19 (08): 120-121, 125). The vitamin C ethyl ether is a very useful vitamin C derivative, is very stable in chemical substances, is a non-discoloring vitamin C derivative, is an oleophilic and hydrophilic amphoteric substance, and greatly expands the application range of the vitamin C ethyl ether, and is particularly applied to daily chemicals.

As one of the main antioxidants of human body, vitamin E can block the chain reaction of free radicals in cells, protect the skin from being damaged by the free radicals generated by ultraviolet irradiation, reduce the generation of wrinkles and prevent the skin from being aged in advance. The external preparation has effects in improving skin elasticity, keeping smooth and moist, and preventing skin keratinization. Vitamin E also promotes healing of scars and reduces pigmentation. Foreign scholars also achieve certain achievements in treating chloasma by using vitamin E in combination (reference document: xu Yan Nu, sha Xianzheng. Vitamin E shallow function analysis [ J ]. Chinese medical engineering, 2014,22 (03): 186-187).

According to an embodiment of the present invention, the cosmetic composition of the present invention further comprises phospholipids and cholesterol.

According to an embodiment of the present invention, the phospholipids in the skin care liposome gel of the cosmetic composition of the present invention are natural phospholipids, semisynthetic phospholipids, fully synthetic phospholipids, a mixture of natural phospholipids and semisynthetic phospholipids, or a mixture of natural phospholipids and fully synthetic phospholipids, and the content of phosphatidylcholine is 50% to 99%.

According to an embodiment of the present invention, the cosmetic composition of the present invention further comprises a gel matrix, wherein the gel matrix comprises: carbomer, a humectant, a transdermal absorption enhancer, and a pH regulator. Therefore, the effects of the cosmetic composition on the aspects of maintaining beauty and keeping young, whitening and protecting skin, delaying senility and maintaining beauty and removing freckles can be further improved.

According to an embodiment of the present invention, in the gel matrix of the cosmetic composition of the present invention, the humectant is selected from: at least one of lentinan, aloe extract, and biological carbohydrate gum-1.

According to an embodiment of the present invention, in the gel base of the cosmetic composition of the present invention, the pH adjusting agent is selected from: triethanolamine.

According to an embodiment of the present invention, in the gel base of the cosmetic composition of the present invention, the transdermal absorption enhancer is selected from the group consisting of: oat beta glucan.

According to an embodiment of the invention, in the cosmetic composition of the invention, the moisturizer is selected from the group consisting of: at least one of lentinan, aloe extract, and biological carbohydrate gum-1.

The lentinan is an effective active component extracted from high-quality lentinus edodes sporocarp, the active component in the lentinan is beta- (1-3) -D-glucan with branches, a main chain is composed of beta- (1-3) -connected glucosyl, the beta- (1-6) -connected glucosyl is randomly distributed along the main chain, and the lentinan is in a comb-shaped structure. Lentinan is off-white powder, mostly acidic polysaccharide, soluble in water and dilute alkali, especially soluble in hot water, insoluble in organic solvents such as ethanol, acetone, ethyl acetate, and diethyl ether, and its aqueous solution is transparent and viscous. Clinical and pharmacological research shows that lentinan has the functions of resisting virus, resisting tumor, regulating immunity, stimulating the formation of interferon, etc. In the invention, the humectant lentinan can be extracted by using commercially available lentinus edodes as a raw material through the processes of water extraction and alcohol precipitation, dilute alkali extraction, dilute acid extraction, an enzyme method and the like. The best process research on lentinan extraction by water extraction and alcohol precipitation [ J ] Qingdao university report of agriculture (Nature science edition), 2020,37 (01): 43-46, chengxiangxiang, qi Jingqi, ching, gao et Jun, flash extraction lentinan process optimization based on orthogonal test method [ J ] food research and development, 2019,40 (20): 70-74, tianguang, optimization of lentinan extraction process [ J ] Yanan university report, 2002,21 (2): 46-47, wangzhong, zyongliang, lentinan separation optimal process and optimal raw material discussion [ J ]. 2000,31 (8): 584-585), which are incorporated herein by reference in their entirety. Alternatively, in the present invention, the raw material of lentinan can also be obtained by commercially available methods.

Aloe vera (Aloe vera) is also known as Aloe vera, aloe barbadensis or Aloe americana, commonly known as Aloe vera. Aloe leaves contain abundant yellow juice and have been used as laxatives or stomachic agents since ancient times. The aloe leaves also contain a clear gel, where the majority of the active ingredient is present. The active component extracted from aloe has wide application, can treat various skin diseases and skin injury in medicine, and has the effects of resisting bacteria, diminishing inflammation and enhancing disease resistance; has good curative effect on cardiovascular and cerebrovascular diseases and gastrointestinal ulcer, is a potential anti-tumor, anti-cancer and AIDS-treating medicament, can remarkably regulate the immune system of a human body, and is considered as a high-efficiency nontoxic immunomodulator. In the present invention, the raw material of the aloe vera extract can be commercially available.

Biogel-1 is a biological polysaccharide, is generally called fucosan because of a large amount of fucose in the molecule, is a novel moisturizing agent, wrinkle removing agent, skin repairing agent and skin feeling regulator in the cosmetic industry, and has the main functions: moisturizing, improving skin feel, relieving skin, and providing soft, smooth and pleasant skin feel for skin. In the present invention, the biosaccharide gum-1 raw material can be commercially available.

According to an embodiment of the present invention, in the cosmetic composition of the present invention, the pH adjusting agent is selected from: triethanolamine.

According to an embodiment of the present invention, in the cosmetic composition of the present invention, the transdermal absorption enhancer is selected from the group consisting of: oat beta glucan.

According to the embodiment of the invention, the beta- (1 → 3,1 → 4) glucan in the oat is called oat beta-glucan in short, and is a non-starch polysaccharide existing in the cell walls of the endosperm and aleurone layer of the oat. Oat beta glucan is a short chain glucan of relatively small molecular mass. The oat beta-glucan has excellent anti-aging effect, and can smooth fine wrinkles, improve skin elasticity and improve skin texture. In addition, the oat beta-glucan can proliferate mouse lymphocytes, enhance the capability of mice to resist bacterial invasion and has an immunoregulation effect. In the present invention, oat beta-glucan is commercially available.

According to an embodiment of the present invention, the cosmetic composition of the present invention further comprises a gel base, phospholipids, cholesterol, an appropriate amount of phosphate buffer solution, and an appropriate amount of deionized water.

The prescription and the preparation method of the phosphate buffer solution provided by the invention are as follows:

7.9g NaCl, 0.2g KCl and 0.24g KH were weighed out separately ₂ PO ₄ 、1.8g K ₂ HPO ₄ Dissolving in 800ml deionized water, adjusting the pH value of the solution to 7.4 by using HCl, finally adding deionized water to constant volume to 1L to obtain phosphate buffer solution, and storing the prepared solution in a refrigerator at 4 ℃; or: 7.9g NaCl, 0.2g KCl and 1.44g Na were weighed out separately ₂ HPO ₄ 、1.8g K ₂ HPO ₄ Dissolving in 800ml deionized water, adjusting the pH value of the solution to 7.4 by using HCl, finally adding deionized water to a constant volume of 1L to obtain a phosphate buffer solution, and storing the prepared solution in a refrigerator at 4 ℃.

According to the embodiment of the invention, the cosmetic composition can be further prepared into liposome gel for skin care.

Thus, according to a second aspect of the present invention, there is provided a liposome gel for skin care of a cosmetic composition. The liposome is used as a common nano administration carrier, and the liposome has unique advantages of skin administration, can promote the penetration capability of the medicament to the skin and enhance the dermal retention performance of the medicament. The gel can be tightly attached to the action part for a long time, has good biological attachment, and has the characteristics of simple preparation, comfortable use, good air permeability, high bioavailability, good stability, less adverse reaction, convenient use and the like. The liposome gel mixes the liposome and the gel matrix, thereby increasing the stability of the liposome, improving the applicability of the liposome as an external preparation, and providing slow release and controlled release of the encapsulated drug.

According to an embodiment of the present invention, the skin care liposome gel of the cosmetic composition of the present invention comprises, in parts by weight: 0.2 to 1 weight portion of grape seed extract, 0.2 to 1 weight portion of white peony root extract, 0.1 to 0.3 weight portion of seal fruit oil, 0.15 to 1.5 weight portions of vitamin C ethyl ether, 0.05 to 0.5 weight portion of vitamin E, 8 to 16 weight portions of phospholipid, 1.6 to 3.2 weight portions of cholesterol, 0.5 to 1.2 weight portions of humectant, 1.2 to 2.5 weight portions of carbomer, 0.1 to 1 weight portion of transdermal absorption enhancer, 40 to 50 weight portions of phosphate buffer solution, a proper amount of pH regulator to regulate the pH value to be 6.0 to 7.0, and finally deionized water is added until the total weight portion of the prescription is 100 weight portions.

According to an embodiment of the present invention, the skin care liposome gel of the cosmetic composition of the present invention preferably comprises, in parts by weight: 0.25 to 0.8 portion of grape seed extract, 0.25 to 0.8 portion of white peony root extract, 0.15 to 0.25 portion of Indian fruit oil, 0.4 to 0.8 portion of vitamin C ethyl ether, 0.15 to 0.45 portion of vitamin E, 9 to 15 portions of phospholipid, 1.8 to 3 portions of cholesterol, 0.6 to 1.2 portions of humectant, 1.6 to 2.4 portions of carbomer, 0.3 to 0.8 portion of transdermal absorption enhancer, 42 to 48 portions of phosphate buffer solution and a proper amount of pH regulator to regulate the pH value to be 6.1 to 6.9, and finally deionized water is added until the total weight portion of the prescription is 100 portions.

According to an embodiment of the present invention, the skin care liposome gel of the cosmetic composition of the present invention comprises, in parts by weight: 0.25 part by weight of grape seed extract, 0.25 part by weight of white peony root extract, 0.15 part by weight of crabapple oil, 0.4 part by weight of vitamin C ethyl ether, 0.15 part by weight of vitamin E, 9 parts by weight of phospholipid, 1.8 parts by weight of cholesterol, 0.6 part by weight of lentinan, 1.6 parts by weight of carbomer, 0.3 part by weight of oat beta-glucan, 42 parts by weight of phosphate buffer solution and a proper amount of triethanolamine to adjust the pH value to 6.1, and finally deionized water is added until the total weight of the formula is 100 parts by weight.

According to an embodiment of the present invention, the skin care liposome gel of the cosmetic composition of the present invention comprises, by weight: 0.8 part by weight of grape seed extract, 0.8 part by weight of white peony root extract, 0.25 part by weight of crabapple oil, 0.8 part by weight of vitamin C ethyl ether, 0.45 part by weight of vitamin E, 15 parts by weight of phospholipid, 3 parts by weight of cholesterol, 1.2 parts by weight of lentinan, 2.4 parts by weight of carbomer, 0.8 part by weight of oat beta-glucan, 48 parts by weight of phosphate buffer solution and a proper amount of triethanolamine to adjust the pH value to 6.9, and finally adding deionized water until the total weight of the formula is 100 parts by weight.

According to an embodiment of the present invention, the skin care liposome gel of the cosmetic composition of the present invention comprises, by weight: 0.5 part by weight of grape seed extract, 0.5 part by weight of white peony root extract, 0.2 part by weight of crabapple oil, 0.5 part by weight of vitamin C ethyl ether, 0.25 part by weight of vitamin E, 10 parts by weight of phospholipid, 2 parts by weight of cholesterol, 1 part by weight of lentinan, 2 parts by weight of carbomer, 0.5 part by weight of oat beta-glucan, 45 parts by weight of phosphate buffer solution and a proper amount of triethanolamine are added to adjust the pH value to 6.6, and finally deionized water is added to the total weight of the formula to be 100 parts by weight.

According to an embodiment of the present invention, the skin care liposome gel of the cosmetic composition of the present invention comprises, by weight: 0.5 part by weight of grape seed extract, 0.5 part by weight of white peony root extract, 0.2 part by weight of crabapple oil, 0.5 part by weight of vitamin C ethyl ether, 0.25 part by weight of vitamin E, 10 parts by weight of phospholipid, 2 parts by weight of cholesterol, 0.8 part by weight of biogum glycocoll-1, 2 parts by weight of carbomer, 0.5 part by weight of oat beta-glucan, 42 parts by weight of phosphate buffer solution and a proper amount of triethanolamine to adjust the pH value to 6.5, and finally adding deionized water until the total weight of the formula is 100 parts by weight.

According to an embodiment of the present invention, the skin care liposome gel of the cosmetic composition of the present invention comprises, by weight: 0.5 part by weight of grape seed extract, 0.5 part by weight of white peony root extract, 0.2 part by weight of crabapple oil, 0.5 part by weight of vitamin C ethyl ether, 0.25 part by weight of vitamin E, 10 parts by weight of phospholipid, 2 parts by weight of cholesterol, 0.9 part by weight of aloe extract, 2 parts by weight of carbomer, 0.5 part by weight of oat beta-glucan, 46 parts by weight of phosphate buffer solution and a proper amount of triethanolamine to adjust the pH value to 6.3, and finally deionized water is added to the total weight of the formula to be 100 parts by weight.

According to an embodiment of the present invention, the skin care liposome gel of the cosmetic composition of the present invention comprises, by weight: 0.6 part by weight of grape seed extract, 0.6 part by weight of white peony root extract, 0.24 part by weight of crabapple oil, 0.6 part by weight of vitamin C ethyl ether, 0.3 part by weight of vitamin E, 12 parts by weight of phospholipid, 2.4 parts by weight of cholesterol, 0.9 part by weight of lentinan, 2.4 parts by weight of carbomer, 0.6 part by weight of oat beta-glucan, 45 parts by weight of phosphate buffer solution and a proper amount of triethanolamine to adjust the pH value to 6.2, and finally deionized water is added until the total weight of the formula is 100 parts by weight.

According to an embodiment of the present invention, the skin care liposome gel of the cosmetic composition of the present invention preferably comprises, in parts by weight: 0.2 part by weight of grape seed extract, 0.2 part by weight of white peony root extract, 0.1 part by weight of crabapple oil, 0.15 part by weight of vitamin C ethyl ether, 0.05 part by weight of vitamin E, 8 parts by weight of phospholipid, 1.6 parts by weight of cholesterol, 0.5 part by weight of lentinan, 1.2 parts by weight of carbomer, 0.1 part by weight of oat beta-glucan, 40 parts by weight of phosphate buffer solution and a proper amount of pH regulator to regulate the pH value to 6.0, and finally deionized water is added until the total weight of the formula is 100 parts by weight.

According to an embodiment of the present invention, the skin care liposome gel of the cosmetic composition of the present invention comprises, by weight: 1 part by weight of grape seed extract, 1 part by weight of white peony root extract, 0.3 part by weight of crabapple oil, 1.5 parts by weight of vitamin C ethyl ether, 0.5 part by weight of vitamin E, 16 parts by weight of phospholipid, 3.2 parts by weight of cholesterol, 1.2 parts by weight of biogum glycocoll-1, 2.5 parts by weight of carbomer, 1 part by weight of oat beta-glucan, 50 parts by weight of phosphate buffer solution and a proper amount of pH regulator to adjust the pH value to 7.0, and finally adding deionized water until the total weight of the formula is 100 parts by weight.

According to a third aspect of the present invention, the present invention provides a liposome gel for skin care of a cosmetic composition and a preparation method thereof, wherein the liposome gel has a formula of: 0.2 to 1 weight portion of grape seed extract, 0.2 to 1 weight portion of white peony root extract, 0.1 to 0.3 weight portion of seal fruit oil, 0.15 to 1.5 weight portions of vitamin C ethyl ether, 0.05 to 0.5 weight portion of vitamin E, 8 to 16 weight portions of phospholipid, 1.6 to 3.2 weight portions of cholesterol, 0.5 to 1.2 weight portions of humectant, 1.2 to 2.5 weight portions of carbomer, 0.1 to 1 weight portion of transdermal absorption enhancer, a proper amount of phosphate buffer solution, a proper amount of pH regulator to regulate the pH value to be 6.0 to 7.0, and finally deionized water is added until the total weight portion of the prescription is 100 weight portions.

The preparation method of the liposome gel comprises the following steps:

step (1): dissolving 4-8 parts by weight of phospholipid and 0.8-1.6 parts by weight of cholesterol in diethyl ether, removing the diethyl ether under reduced pressure, adding 0.15-1.5 parts by weight of vitamin C ethyl ether and 0.2-1 part by weight of grape seed extract, dissolving the mixture in deionized water, carrying out rotary hydration, carrying out ultrasonic treatment by using a probe type ultrasonic instrument, and carrying out volume metering to obtain a first liposome suspension as a first mixture;

step (2): mixing 4-8 parts by weight of phospholipid, 0.8-1.6 parts by weight of cholesterol, 0.05-0.5 part by weight of vitamin E, 0.2-1 part by weight of white peony root extract and 0.1-0.3 part by weight of adducted peanut oil, dissolving the mixture in ethanol to form a mixed solution, quickly injecting the mixed solution into 40-50 parts by weight of phosphate buffer solution at the temperature of 60 ℃, removing the ethanol under reduced pressure, and performing ultrasonic treatment by using a probe type ultrasonic instrument to perform constant volume to obtain a second liposome suspension as a second mixture;

and (3): dispersing 1.2-2.5 parts by weight of carbomer in deionized water, and fully swelling; adding a pH regulator to regulate the pH value to 6.0-7.0, adding 0.5-1.2 parts by weight of humectant and 0.1-1 part by weight of transdermal absorption enhancer, and fully and uniformly stirring to obtain a third mixture;

and (4): and (3) adding deionized water into the first mixture prepared in the step (1), the second mixture prepared in the step (2) and the third mixture prepared in the step (3) to make the total weight be 100 parts by weight, and fully mixing to obtain the skin-care liposome gel of the cosmetic composition.

According to the embodiment of the invention, in the preparation method in the step (1) and/or the step (2), the ultrasonic treatment is performed by using a probe-type ultrasonic instrument, and the ultrasonic treatment is performed for 15min at 250W (25%) power in an ice bath environment, and the ultrasonic treatment is stopped for 2 seconds every 1 second.

According to the embodiment of the invention, the invention provides a skin-care liposome gel of a cosmetic composition and a preparation method thereof, and preferably, the formulation of the liposome gel is as follows: 0.25 to 0.8 portion of grape seed extract, 0.25 to 0.8 portion of white peony root extract, 0.15 to 0.25 portion of Indian fruit oil, 0.4 to 0.8 portion of vitamin C ethyl ether, 0.15 to 0.45 portion of vitamin E, 9 to 15 portions of phospholipid, 1.8 to 3 portions of cholesterol, 0.6 to 1.2 portions of humectant, 1.6 to 2.4 portions of carbomer, 0.3 to 0.8 portion of transdermal absorption enhancer, 42 to 48 portions of phosphate buffer solution and a proper amount of pH regulator to regulate the pH value to be 6.1 to 6.9, and finally deionized water is added until the total weight portion of the prescription is 100 portions.

Preferably, the preparation method comprises the following steps:

step (1): dissolving 4.5-7.5 parts by weight of phospholipid and 0.9-1.5 parts by weight of cholesterol in diethyl ether, removing the diethyl ether under reduced pressure, adding 0.4-0.8 part by weight of vitamin C ethyl ether and 0.25-0.8 part by weight of grape seed extract, dissolving the mixture in deionized water, carrying out rotary hydration for 15 minutes, carrying out ultrasonic treatment by using a probe type ultrasonic instrument, and fixing the volume to obtain a first liposome suspension solution which is a first mixture;

step (2): mixing 4.5-7.5 parts by weight of phospholipid, 0.9-1.5 parts by weight of cholesterol, 0.15-0.45 part by weight of vitamin E, 0.25-0.8 part by weight of white peony root extract and 0.15-0.25 part by weight of seal fruit oil, dissolving in ethanol to form a mixed solution, quickly injecting the mixed solution into 42-48 parts by weight of phosphate buffer solution at the temperature of 60 ℃, removing the ethanol under reduced pressure, and performing ultrasonic treatment by using a probe type ultrasonic instrument to perform constant volume to obtain a second liposome mixed suspension as a second mixture;

and (3): dispersing 1.2-2.5 parts by weight of carbomer in deionized water, and fully swelling; adding a pH regulator to regulate the pH value to 6.1-6.9, adding 0.5-1.2 parts by weight of humectant and 0.1-1 part by weight of transdermal absorption enhancer, and fully and uniformly stirring to obtain a third mixture;

According to the embodiment of the invention, in the preparation method of the step (1) and/or the step (2), the probe type ultrasonic instrument is used for ultrasonic treatment for 15min at 250W (25%) power in an ice bath environment, and the ultrasonic treatment is stopped for 2 seconds every 1 second.

According to an embodiment of the present invention, in the method for preparing a cosmetic composition according to the present invention, the moisturizer is selected from the group consisting of: at least one of lentinan, aloe extract and biological carbohydrate gum-1.

According to an embodiment of the present invention, in the method for preparing a cosmetic composition according to the present invention, the pH adjusting agent is selected from: triethanolamine.

According to an embodiment of the present invention, in the method for preparing a cosmetic composition according to the present invention, the transdermal absorption enhancer is selected from the group consisting of: oat beta glucan.

According to an embodiment of the present invention, the present invention provides a liposome gel for skin care of a cosmetic composition and a preparation method thereof, wherein the liposome gel has a formula of: 0.5g of grape seed extract, 0.5g of white peony root extract, 0.2g of crabapple oil, 0.5g of vitamin C ethyl ether, 0.25g of vitamin E, 10g of phospholipid, 2g of cholesterol, 1g of lentinan, 2g of carbomer, 0.5g of oat beta-glucan, 45g of phosphate buffer solution and a proper amount of triethanolamine are used for adjusting the pH value to be 6.6, and finally deionized water is added until the total weight of the formula is 100g.

According to a specific embodiment of the present invention, the preparation method comprises the steps of:

step (1): dissolving 5g of phospholipid and 1g of cholesterol in 30mL of diethyl ether, removing the diethyl ether in a water bath at 30 ℃ under reduced pressure, adding 0.5g of vitamin C ethyl ether and 0.5g of grape seed extract, dissolving the mixture in 20mL of deionized water, carrying out rotary hydration for 15 minutes, carrying out ultrasonic treatment for 15 minutes at 250W (25%) power in an ice bath environment by using a probe type ultrasonic instrument, stopping ultrasonic treatment for 2 seconds every 1 second, and finally carrying out constant volume to 25mL to obtain a first liposome suspension solution which is a first mixture;

step (2): mixing 5g of phospholipid, 1g of cholesterol, 0.25g of vitamin E, 0.25g of white peony root extract and 0.2g of crataegus fruit oil, dissolving in 10mL of ethanol to form a mixed solution, quickly injecting the mixed solution into a phosphate buffer solution with the temperature of 60 ℃ and the weight of 45g, removing the ethanol under reduced pressure, carrying out ultrasonic treatment for 15min by using a probe type ultrasonic instrument in an ice bath environment at the power of 250W (25%), stopping ultrasonic treatment for 2 seconds every 1 second, and finally fixing the volume to 50mL to obtain a second liposome suspension as a second mixture;

and (3): dispersing 2g carbomer in deionized water, and fully swelling; adding triethanolamine to adjust pH to 6.6, adding lentinan 1g and oat beta-glucan 0.5g, and stirring to obtain a third mixture;

On the basis of the common knowledge in the field, the above preferred conditions can be combined randomly to obtain the preferred embodiments of the invention. The reagents and starting materials used in the present invention are commercially available.

According to an embodiment of the present invention, the weight of the parts of the present invention may be expressed in tons.

According to the specific embodiment of the present invention, the weight of the parts of the present invention may be kilograms or kilograms.

According to an embodiment of the present invention, the weight of the parts may be in grams.

According to an embodiment of the present invention, the weight of the parts may be milligrams.

According to an embodiment of the present invention, the weight of the parts may be microgram.

The quality of the skin-care liposome gel of the cosmetic composition prepared by the invention is detected, and the appearance detection shows that the liposome gel is milky semitransparent semisolid, has proper consistency, is fine and uniform, and has good spreadability; centrifugal experiments show that: the liposome gel has no layering phenomenon; the cold and heat resistance experiment shows that: the liposome gel provided by the invention has no layering phenomenon.

According to a fourth aspect of the present invention, the present invention provides a use of a cosmetic composition for preparing a cosmetic capable of simultaneously beautifying, whitening, skin-care, delaying aging, beautifying and removing freckles.

The cosmetic composition provided by the invention is used for preparing liposome gel, and has no irritation to skin. In animal pharmacodynamic experiments for reducing wrinkle formation and delaying skin aging, the results show that: the effect and the efficacy of the cosmetic composition liposome gel provided by the invention on the aspects of maintaining beauty and keeping young, reducing the formation of wrinkles and delaying the aging process of skin are obviously better than those of the experimental group of the gel in the comparative example. In the freckle removing effect test, after the cosmetic composition product is applied, the melanin content in human skin can be obviously reduced, which indicates that the cosmetic composition liposome gel can be applied to beautify and remove freckles.

The positive progress effects of the invention are as follows:

(1) In the formula of the cosmetic composition, the moisturizing agent, the pH regulator, the carbomer and the transdermal absorption enhancer form the skin-care gel matrix, and the gel matrix has no greasy feeling, is easy to coat and spread and is easy to wash; can absorb the tissue exudate without interfering the normal function of the skin; the consistency is low, which is beneficial to the release of the drug, in particular to the release of the water-soluble drug.

(2) The invention creatively selects the oat beta-glucan as the transdermal absorption enhancer, and plays a very good role in promoting the permeation.

(3) The cosmetic composition of the present invention was prepared from the liposome solution of the first mixture containing vitamin C ethyl ether, and the optimal preparation method was determined to be a thin film dispersion method by comparing the preparation methods of 4.

(4) The product of the invention has regular morphological structure, moderate size, good dispersibility, uniform particle size distribution, high encapsulation efficiency and good stability.

(5) Compared with the common gel, the liposome gel provided by the invention encapsulates each active component of the cosmetics in the liposome, so that the local skin irritation can be reduced, the components can be concentrated to form a storage, the retention of each component in the skin is increased, a slow release effect is formed, and the effects of each component for beautifying, whitening, protecting skin, delaying aging and beautifying and removing freckles are better exerted.

Drawings

FIG. 1 is a transmission electron microscope image of a liposome suspension obtained in example 1 of the present invention;

FIG. 2 is a transmission electron micrograph of the liposomal suspension obtained in example 2 of the invention.

Detailed Description

The invention is further illustrated by the following examples, which are not intended to limit the scope of the invention. The experimental methods without specifying specific conditions in the following examples were selected according to the conventional methods and conditions, or according to the commercial instructions.

Example 1 formulation and preparation of a first mixture according to the invention

Formulation and preparation method (film dispersion method) of the first mixture of the liposome fluid (1):

dissolving 5g of phospholipid and 1g of cholesterol in 30mL of diethyl ether, removing the diethyl ether in a water bath at 30 ℃ under reduced pressure, then adding 0.5g of vitamin C ethyl ether and 0.5g of grape seed extract, dissolving the mixture in 20mL of deionized water, performing rotary hydration for 15 minutes, performing ultrasonic treatment for 15 minutes by using a probe type ultrasonic instrument in an ice bath environment at the power of 250W (25%) for 2 seconds after 1 second of ultrasonic treatment, and finally performing constant volume to 25mL to obtain a first mixture which is marked as a liposome liquid (1). The encapsulation efficiency (EE%) was determined by HPLC using vitamin C ethyl ether as an index. The method comprises the following specific steps:

chromatographic conditions are as follows:

the mobile phase and the proportion are as follows: methanol: 0.025 mol/potassium dihydrogen phosphate solution =75 (v/v)

And (3) chromatographic column: c18 column, 250X 4.6mm,5 μm

Flow rate: 1ml/min

Column temperature: 30 deg.C

Detection wavelength: 244nm

The method comprises the following steps: and (3) taking 5mL of the liposome liquid (1) in an mw3500 dialysis bag, adding 200mL of deionized water, rotating at 150r/min in a constant-temperature water bath at 30 ℃, taking the measured content of an external solution after 5 hours as the content of free drugs, and calculating the encapsulation rate of the vitamin C ethyl ether. And (3) measuring the particle size and the potential of the liposome by using a Malvern particle sizer: after 8-fold dilution of the liposome fluid (1) of the first mixture, the particle size and potential were determined, each characterized as:

EE％	particle size	PDI	zeta potential
				85.46％	167.5	0.283	-67.6

Formulation and preparation method of the first mixture of the lipid body fluid (2) (reverse evaporation method):

dissolving 5g of phospholipid and 1g of cholesterol in 30mL of diethyl ether to form diethyl ether solution, dissolving 0.5g of vitamin C ethyl ether and 0.5g of grape seed extract in 20mL of deionized water, then adding the solution into the diethyl ether solution, carrying out water bath ultrasound for 30min, uniformly mixing, removing the diethyl ether at 30 ℃ under reduced pressure, carrying out ultrasound for 15min by using a probe type ultrasound instrument in an ice bath environment at the power of 250W (25%), stopping for 2 s every 1 second of ultrasound, and finally carrying out constant volume to 25mL to obtain the liposome liquid (2) of the first mixture. The encapsulation efficiency was measured by HPLC using vitamin C ethyl ether as an index, and the measurement method was the same as that of the liposome liquid (1) of the first mixture. Measuring the particle size and potential of the liposome by a Malvern particle sizer: after 8-fold dilution of the liposome fluid (2) of the first mixture, the particle size and potential were determined, each characterized by:

EE％	particle size	PDI	zeta potential
				42.44％	180.2	0.226	-62.9

Prescription and preparation method of the first mixture of lipid body fluid (3) (multiple emulsion method):

dissolving 5g of phospholipid and 1g of cholesterol in 30mL of diethyl ether to form an ether solution, dissolving 0.5g of vitamin C ethyl ether and 0.5g of grape seed extract in 10mL of deionized water, adding the solution into the ether solution, carrying out water bath ultrasonic treatment for 30min, uniformly mixing, then placing the emulsion in 10mL of deionized water, carrying out ultrasonic treatment for 30min, removing the diethyl ether at 30 ℃ under reduced pressure, carrying out ultrasonic treatment for 15min at a power of 250W (25%) in an ice bath environment by using a probe type ultrasonic instrument, stopping the ultrasonic treatment for 2 s every 1 second, and finally carrying out constant volume to 25mL to obtain the liposome liquid (3) of the first mixture.

The encapsulation efficiency was measured by HPLC using vitamin C ethyl ether as an index, and the measurement method was the same as that of the liposome liquid (1) of the first mixture. Measuring the particle size and potential of the liposome by a Malvern particle sizer: after 8-fold dilution of the liposome fluid (3) of the first mixture, the particle size and potential were determined, each of which is characterized by:

EE％	particle size	PDI	zeta potential
				22.28％	171.4	0.204	-67.2

Formulation and preparation of the first mixture of lipid fluids (4) (ether infusion method):

dissolving 5g of phospholipid, 1g of cholesterol in 10ml of diethyl ether to form a mixed solution A; dissolving 0.5g of vitamin C ethyl ether and 0.5g of grape seed extract by using 10mL of deionized water to form a mixed solution B, after the temperature is constant at 60 ℃, injecting the mixed solution A into the mixed solution B by using a fine needle at the speed of 0.2mL/min, stirring for 30min, carrying out ultrasonic treatment for 15min by using a probe type ultrasonic instrument in an ice bath environment at the power of 250W (25%), stopping ultrasonic treatment for 2 seconds every 1 second, and finally carrying out constant volume treatment to 25mL to obtain the liposome liquid (4) of the first mixture.

The encapsulation efficiency was measured by HPLC using vitamin C ethyl ether as an index, and the measurement method was the same as that of the liposome liquid (1) of the first mixture. Measuring the particle size and potential of the liposome by a Malvern particle sizer: after 8-fold dilution of the liposome fluid (4) of the first mixture, the particle size and potential were determined, each characterized by:

EE％	particle size	PDI	zeta potential
				52.07％	175.3	0.218	-65.3

The method for preparing the first mixture according to the present invention was determined to be a thin film dispersion method by measuring the encapsulation ratio, particle size and potential of the liposome solution of each of the first mixtures, and the method for preparing the same was as follows:

dissolving 4-8 parts by weight of phospholipid and 0.8-1.6 parts by weight of cholesterol in diethyl ether, removing the diethyl ether under reduced pressure, adding 0.15-1.5 parts by weight of vitamin C ethyl ether and 0.2-1 part by weight of grape seed extract, dissolving the mixture in deionized water, carrying out rotary hydration, carrying out ultrasonic treatment by using a probe type ultrasonic instrument, and carrying out volume metering to obtain a first liposome suspension which is a first mixture.

Preferably, the first mixture of the present invention is formulated and prepared by the following steps:

dissolving 4.5-7.5 parts by weight of phospholipid and 0.9-1.5 parts by weight of cholesterol in diethyl ether, removing the diethyl ether under reduced pressure, adding 0.4-0.8 part by weight of vitamin C ethyl ether and 0.25-0.8 part by weight of grape seed extract, dissolving the mixture in deionized water, performing rotary hydration for 15 minutes, performing ultrasonic treatment by using a probe type ultrasonic instrument, and performing constant volume to obtain a first liposome suspension as a first mixture.

dissolving 5g of phospholipid and 1g of cholesterol in 30mL of diethyl ether, removing the diethyl ether in a water bath at 30 ℃ under reduced pressure, adding 0.5g of vitamin C ethyl ether and 0.5g of grape seed extract, dissolving the mixture in 20mL of deionized water, carrying out rotary hydration for 15 minutes, carrying out ultrasonic treatment for 15 minutes at 250W (25%) power in an ice bath environment by using a probe type ultrasonic instrument, stopping ultrasonic treatment for 2 seconds every 1 second, and finally carrying out constant volume to 25mL to obtain a first liposome suspension which is a first mixture. The first mixture is observed by a transmission electron microscope, and is observed to be spherical or nearly spherical small vesicles under the electron microscope, the morphology is regular, the dispersibility is good, the particle size distribution is uniform, and the result is shown in figure 1.

EXAMPLE 2 formulation and preparation of the second mixture of the invention

Formulation and preparation method (thin film dispersion method) of the second mixture of the lipid fluid (5):

mixing 5g of phospholipid, 5g of cholesterol, 0.25g of vitamin E, 0.5g of white peony root extract and 0.2g of seal fruit oil, dissolving in 10mL of ethanol to form a mixed solution, then removing the ethanol under reduced pressure, carrying out ultrasonic treatment for 15min at the power of 250W (25%) in an ice bath environment by using a probe type ultrasonic instrument, stopping the ultrasonic treatment for 2 seconds every 1 second, and finally carrying out constant volume treatment to 50mL to obtain the liposome liquid (5) of the second mixture. The vitamin E is used as an index, and the encapsulation efficiency is measured by adopting an ultraviolet spectrophotometry.

The determination method comprises the following steps:

ultraviolet conditions: shimadzu 1700 uv spectrophotometer;

the method comprises the following steps: accurately weighing 50mg of vitamin E reference substance with anhydrous ethanol as blank, diluting with anhydrous ethanol, fixing volume in 50mL brown measuring flask, scanning at 200-400nm wavelength, wherein vitamin E has maximum absorbance at 282nm, and anhydrous ethanol solution of radix Paeoniae alba extract and Plukenetia volubilis oil has no absorption peak at 282 nm. Collecting 5ml of the liposome solution (5) of the second mixture, centrifuging at 15000r/min at 4 deg.C for 60min, collecting supernatant 1ml, adding anhydrous ethanol 4ml, filtering, measuring vitamin E concentration, and calculating encapsulation efficiency.

Measuring the particle size and potential of the liposome by a Malvern particle sizer: after 8-fold dilution of the liposome fluid (5) of the second mixture, the particle size and potential were determined, each of which is characterized by:

EE％	particle size	PDI	zeta potential
				39.7％	165.8	0.233	-69.4

Formulation and preparation method (infusion method) of a second mixture of a lipid body fluid (6):

mixing 5g of phospholipid, 1g of cholesterol, 0.25g of vitamin E, 0.5g of white peony root extract and 0.2g of adducted fruit oil, dissolving in 10mL of ethanol to form a mixed solution, quickly injecting the mixed solution into a phosphate buffer solution with the temperature of 60 ℃ and the weight of 45g, removing the ethanol under reduced pressure, carrying out ultrasonic treatment for 15min at the power of 250W (25%) by using a probe type ultrasonic instrument in an ice bath environment, stopping the ultrasonic treatment for 2 seconds every 1 second, and finally fixing the volume to 50mL to obtain a second liposome suspension which is a second mixture. And measuring the encapsulation efficiency of the second mixture by using vitamin E as an index and adopting an ultraviolet spectrophotometry, and the measuring method is the same as that of the liposome liquid (5) of the second mixture. Measuring the particle size and potential of the liposome by a Malvern particle sizer: after 8-fold dilution of the liposome fluid (6) of the second mixture, the particle size and potential were determined, each characterized by:

EE％	particle size	PDI	zeta potential
				82.11％	172.0	0.256	-66.3

The method for preparing the second mixture according to the present invention was confirmed by measuring the particle size and potential of the liposome fluid of each of the second mixtures described above, and was:

mixing 4-8 parts by weight of phospholipid, 0.8-1.6 parts by weight of cholesterol, 0.05-0.5 part by weight of vitamin E, 0.2-1 part by weight of white peony root extract and 0.1-0.3 part by weight of adducted peanut oil, dissolving in ethanol to form a mixed solution, quickly injecting the mixed solution into 40-50 parts by weight of phosphate buffer solution at the temperature of 60 ℃, removing the ethanol under reduced pressure, and performing ultrasonic treatment by using a probe type ultrasonic instrument to perform constant volume to obtain a second liposome suspension as a second mixture.

Preferably, the second mixture of the invention is formulated and prepared by the following method:

mixing 4.5-7.5 parts by weight of phospholipid, 0.9-1.5 parts by weight of cholesterol, 0.15-0.45 part by weight of vitamin E, 0.25-0.8 part by weight of white peony root extract and 0.15-0.25 part by weight of seal fruit oil, dissolving in ethanol to form a mixed solution, quickly injecting the mixed solution into phosphate buffer solution with the temperature of 60 ℃ and the weight of 42-48 parts by weight, removing the ethanol under reduced pressure, performing ultrasonic treatment by using a probe type ultrasonic instrument, and performing volume fixing to obtain a second liposome suspension as a second mixture.

Preferably, the formulation and preparation method of the second mixture of the present invention is:

mixing 5g of phospholipid, 1g of cholesterol, 0.25g of vitamin E, 0.5g of white peony root extract and 0.2g of adducted fruit oil, dissolving in 10mL of ethanol to form a mixed solution, quickly injecting the mixed solution into a phosphate buffer solution with the temperature of 60 ℃ and the weight of 45g, removing the ethanol under reduced pressure, carrying out ultrasonic treatment for 15min at the power of 250W (25%) by using a probe type ultrasonic instrument in an ice bath environment, stopping the ultrasonic treatment for 2 seconds every 1 second, and finally fixing the volume to 50mL to obtain a second liposome suspension which is a second mixture. The second mixture is observed by a transmission electron microscope, and the observation of the second mixture under the electron microscope has the advantages of regular morphological structure, moderate size, good dispersibility and uniform particle size distribution, and the result is shown in figure 2.

Example 3: the liposome gel and the preparation method thereof

Prescription:

0.25g of grape seed extract, 0.25g of white peony root extract, 0.15g of crabapple oil, 0.4g of vitamin C ethyl ether, 0.15g of vitamin E, 9g of phospholipid, 1.8g of cholesterol, 0.6g of lentinan, 1.6g of carbomer, 0.3g of oat beta-glucan, 42g of phosphate buffer solution and a proper amount of triethanolamine to adjust the pH value to 6.1, and finally deionized water is added until the total weight of the formula is 100g.

The preparation method comprises the following steps:

step (1): dissolving 4.5g of phospholipid and 0.9g of cholesterol in 30mL of diethyl ether, decompressing in a water bath at 30 ℃ to remove the diethyl ether, then adding 0.4g of vitamin C ethyl ether and 0.25g of grape seed extract, dissolving with 20mL of deionized water, performing rotary hydration for 15 minutes, performing ultrasonic treatment for 15 minutes by using a probe type ultrasonic instrument in an ice bath environment at the power of 250W (25%) for 1 second and stopping for 2 seconds every 1 second of ultrasonic treatment, and finally performing constant volume to 25mL to obtain a first liposome suspension as a first mixture;

step (2): mixing 4.5g of phospholipid, 0.9g of cholesterol, 0.15g of vitamin E, 0.25g of white peony root extract and 0.15g of adductor oil, dissolving in 10mL of ethanol to form a mixed solution, quickly injecting the mixed solution into a phosphate buffer solution with the temperature of 60 ℃ and the weight of 42g, removing the ethanol under reduced pressure, carrying out ultrasonic treatment on the mixed solution for 15min by using a probe type ultrasonic instrument in an ice bath environment at the power of 250W (25%), stopping the ultrasonic treatment for 2 seconds every 1 second, and finally fixing the volume to 50mL to obtain a second liposome suspension as a second mixture;

and (3): dispersing 1.6g of carbomer in deionized water, and fully swelling; adding triethanolamine to adjust pH to 6.1, adding lentinan 0.6g and oat beta-glucan 0.3g, and stirring to obtain a third mixture;

and (4): and (3) fully mixing the first mixture prepared in the step (1), the second mixture prepared in the step (2) and the third mixture prepared in the step (3), and adding deionized water until the total weight is 100g to obtain the skin-care liposome gel of the cosmetic composition.

Example 4: the liposome gel and the preparation method thereof

Prescription:

0.8g of grape seed extract, 0.8g of white peony root extract, 0.25g of crabapple oil, 0.8g of vitamin C ethyl ether, 0.45g of vitamin E, 15g of phospholipid, 3g of cholesterol, 1.2g of lentinan, 2.4g of carbomer, 0.8g of oat beta-glucan, 48g of phosphate buffer solution and a proper amount of triethanolamine to adjust the pH value to 6.9, and finally deionized water is added until the total weight of the formula is 100g. The preparation method comprises the following steps:

step (1): dissolving 7.5g of phospholipid and 1.5g of cholesterol in 30mL of diethyl ether, decompressing in a water bath at 30 ℃ to remove the diethyl ether, then adding 0.8g of vitamin C ethyl ether and 0.8g of grape seed extract, dissolving with 20mL of deionized water, performing rotary hydration for 15 minutes, performing ultrasonic treatment for 15 minutes by using a probe type ultrasonic instrument in an ice bath environment at the power of 250W (25%) for 2 seconds every 1 second of ultrasonic treatment, and finally performing constant volume to 25mL to obtain a first liposome suspension as a first mixture;

step (2): mixing 7.5g of phospholipid, 1.5g of cholesterol, 0.45g of vitamin E, 0.8g of white peony root extract and 0.25g of crataegus fruit oil, dissolving in 10mL of ethanol to form a mixed solution, quickly injecting the mixed solution into a phosphate buffer solution with the temperature of 60 ℃ and the weight of 48g, removing the ethanol under reduced pressure, carrying out ultrasonic treatment for 15min by using a probe type ultrasonic instrument in an ice bath environment at the power of 250W (25%), stopping ultrasonic treatment for 2 seconds every 1 second, and finally carrying out constant volume treatment to 50mL to obtain a second liposome suspension as a second mixture;

and (3): dispersing 2.4g carbomer in deionized water, and fully swelling; adding triethanolamine to adjust pH to 6.9, adding lentinan 1.2g and oat beta-glucan 0.8g, and stirring to obtain a third mixture;

Example 5: the liposome gel and the preparation method thereof

Prescription:

0.5g of grape seed extract, 0.5g of white peony root extract, 0.2g of crabapple oil, 0.5g of vitamin C ethyl ether, 0.25g of vitamin E, 10g of phospholipid, 2g of cholesterol, 1g of lentinan, 2g of carbomer, 0.5g of oat beta-glucan, 45g of phosphate buffer solution and a proper amount of triethanolamine are used for adjusting the pH value to be 6.6, and finally deionized water is added until the total weight of the formula is 100g. The preparation method comprises the following steps:

step (1): dissolving 5g of phospholipid and 1g of cholesterol in 30mL of diethyl ether, removing the diethyl ether in a water bath at 30 ℃ under reduced pressure, adding 0.5g of vitamin C ethyl ether and 0.5g of grape seed extract, dissolving the mixture in 20mL of deionized water, carrying out rotary hydration for 15 minutes, carrying out ultrasonic treatment for 15 minutes at 250W (25%) power in an ice bath environment by using a probe type ultrasonic instrument, stopping ultrasonic treatment for 2 seconds every 1 second, and finally carrying out constant volume to 25mL to obtain a first liposome suspension which is a first mixture;

step (2): mixing 5g of phospholipid, 1g of cholesterol, 0.25g of vitamin E, 0.5g of white peony root extract and 0.2g of crataegus fruit oil, dissolving in 10mL of ethanol to form a mixed solution, quickly injecting the mixed solution into a phosphate buffer solution with the temperature of 60 ℃ and the weight of 45g, removing the ethanol under reduced pressure, carrying out ultrasonic treatment for 15min by using a probe type ultrasonic instrument in an ice bath environment at the power of 250W (25%), stopping ultrasonic treatment for 2 seconds every 1 second, and finally fixing the volume to 50mL to obtain a second liposome suspension as a second mixture;

and (3): dispersing 2g of carbomer in deionized water, and fully swelling; adding triethanolamine to adjust pH to 6.6, adding lentinan 1g and oat beta-glucan 0.5g, and stirring to obtain a third mixture;

Example 6: the liposome gel and the preparation method thereof

Prescription:

0.5g of grape seed extract, 0.5g of white peony root extract, 0.2g of crabapple oil, 0.5g of vitamin C ethyl ether, 0.25g of vitamin E, 10g of phospholipid, 2g of cholesterol, 0.8g of biogum-1, 2g of carbomer, 0.5g of oat beta-glucan, 42g of phosphate buffer solution and a proper amount of triethanolamine to adjust the pH value to be 6.5, and finally deionized water is added until the total weight of the formula is 100g.

The preparation method comprises the following steps: the preparation method is the same as that described in example 5.

Example 7: the liposome gel and the preparation method thereof

Prescription:

0.5g of grape seed extract, 0.5g of white peony root extract, 0.2g of crabapple oil, 0.5g of vitamin C ethyl ether, 0.25g of vitamin E, 10g of phospholipid, 2g of cholesterol, 0.9g of aloe extract, 2g of carbomer, 0.5g of oat beta-glucan, 46g of phosphate buffer solution and a proper amount of triethanolamine to adjust the pH value to be 6.3, and finally adding deionized water until the total weight of the formula is 100g.

The preparation method comprises the following steps: the preparation method is the same as that described in example 6.

Example 8: the liposome gel and the preparation method thereof

Prescription:

0.6g of grape seed extract, 0.6g of white peony root extract, 0.24g of crabapple oil, 0.6g of vitamin C ethyl ether, 0.3g of vitamin E, 12g of phospholipid, 2.4g of cholesterol, 0.9g of lentinan, 2.4g of carbomer, 0.6g of oat beta-glucan, 45g of phosphate buffer solution and a proper amount of triethanolamine are used for regulating the pH value to be 6.2, and finally deionized water is added until the total weight of the formula is 100g.

Example 9: the liposome gel and the preparation method thereof

Prescription:

0.2g of grape seed extract, 0.2g of white peony root extract, 0.1g of crabapple oil, 0.15g of vitamin C ethyl ether, 0.05g of vitamin E, 8g of phospholipid, 1.6g of cholesterol, 0.5g of lentinan, 1.2g of carbomer, 0.1g of oat beta-glucan, 40g of phosphate buffer solution and a proper amount of pH regulator to adjust the pH value to be 6.0, and finally deionized water is added until the total weight of the formula is 100g.

Example 10: the liposome gel of the invention and the preparation method

Prescription:

1g of grape seed extract, 1g of white peony root extract, 0.3g of crabapple oil, 1.5g of vitamin C ethyl ether, 0.5g of vitamin E, 16g of phospholipid, 3.2g of cholesterol, 1.2g of bioglycan-1, 2.5g of carbomer, 1g of oat beta-glucan, 50g of phosphate buffer solution and a proper amount of pH regulator to adjust the pH value to 7.0, and finally adding deionized water until the total weight of the formula is 100g.

Example 11: gels as described in the comparative examples

A gel containing grape seed extract, vitamin E, vitamin C ethyl ether, indian fruit oil and white peony root extract and a preparation method thereof are as follows:

prescription: 0.5g of grape seed extract, 0.5g of white peony root extract, 0.2g of crabapple oil, 0.5g of vitamin C ethyl ether, 0.25g of vitamin E, 1g of lentinan and 2g of carbomer, and finally adding deionized water until the total weight of the formula is 100g.

The preparation method comprises the following steps:

(1) Fully swelling 2g of carbomer with 50ml of deionized water, and uniformly stirring to obtain a colorless transparent gel matrix;

(2) Dissolving 0.5g of grape seed extract and 0.5g of vitamin C ethyl ether in a proper amount of deionized water;

(3) Dissolving 0.5g radix Paeoniae alba extract with 2.5mL ethanol, mixing with 0.25g vitamin E and 0.2g Plukenetia volubilis oil uniformly;

(4) Adding the products obtained in the steps (2) and (3) into the gel matrix obtained in the step (1), adding a proper amount of deionized water until the total weight is 100g, and stirring; the gel described in the comparative example was obtained.

The gel described in the above comparative example is a gel containing grape seed extract, vitamin E, vitamin C ethyl ether, crataegus fruit oil, and white peony root extract, and compared with the formulas described in examples 3 to 10 of the present invention, the gel has the technical difference that: (1) The gel prepared in this comparative example is a common gel, not a liposome gel; (2) The gel of the comparative example contains only carbomer in the matrix, and does not contain the combination of carbomer, the pH adjusting agent, the humectant and the transdermal absorption enhancer as the gel matrix.

Example 12: quality control of the liposome gel for skin care of the cosmetic composition prepared according to the present invention and the gel of comparative example described in example 11

(1) And (3) appearance detection: the product should be milky white semi-transparent semi-solid, appropriate in consistency, fine, smooth and uniform, and good in spreadability.

(2) Centrifugation experiments for testing gel stability: 5g of sample is put into a centrifugal tube with scales at the rotating speed of 2500 r.min ^-1 After centrifugation for 30min, the gel should not be delaminated.

(3) Cold and heat resistance test for testing gel stability: 5g of samples are uniformly loaded into a packaging box, sealed and respectively placed in a thermostat at 55 ℃ for 6 hours and a refrigerator at-15 ℃ for 24 hours, and the gel should not be layered.

(4) And (3) detection results:

example 13: the retention amount of vitamin C ethyl ether and vitamin E components in the skin care liposome gel of the cosmetic composition of the invention in the skin is measured

This example compares the liposome gel prepared in example 5 of the present invention (experimental group) with the gel of comparative example described in example 11 of the present invention (control group), and measures the retention amounts of vitamin C ethyl ether and vitamin E in the skin, respectively.

The method comprises the following specific operations:

(1) Preparation of in vitro rat skin: SPF-grade KM male mice weighing about 20g per group of 6 mice. The abdominal hair was carefully shaved with a razor, the mouse was sacrificed by dislocation of cervical vertebrae, the skin was peeled off, subcutaneous tissues, fat, etc. were carefully removed, and the skin was washed with physiological saline and blotted with filter paper for use.

(2) And (3) experimental operation: physiological saline was used as the receiving solution. The stratum corneum faces the supply cell, is placed at the junction of the supply cell and the receiving cell of the Franz diffusion cell, is fixed by a spring clip,the drug is administered after 30min of balancing with the receiving liquid (specification of diffusion cell: 2.5cm diameter of inner ring of sample cell, 3.9cm diameter of outer ring, 8mL sample cell, and 14mL receiving cell). Placing the gel in a drug delivery pool, tightly combining with skin surface, heating in water bath to 32 days 0.5 deg.C, and maintaining at 400 r.min ^-1 Electromagnetic constant-speed stirring. 1mL of receiving solution was removed from the receiving well at 0.5,1,1.5,2,3,4,6,8, 10, 12h and the receiving well was replenished with the same amount of release medium in time. After the sample passes through a 0.45-micron membrane, the content of the drug is measured by adopting a liquid phase, and the cumulative release is calculated.

(3) Skin accumulation amount: after the in vitro percutaneous test is finished, the skin is taken down, the gel is washed by normal saline, and the water is absorbed by filter paper. Cutting skin, soaking in methanol, transferring to 5mL glass homogenizer, homogenizing, diluting with anhydrous methanol in 5mL volumetric flask to constant volume to scale, shaking, filtering with 0.45um microporous membrane, and measuring content of filtrate with liquid phase.

(4) And (3) measuring results:

taking the liposome gel prepared by the prescription and the preparation method described in the embodiment 5 as an example, the results of transdermal experiments show that the liposome gel (embodiment 5) has the effect of promoting the drug to permeate the skin and simultaneously detects the content of vitamin C and vitamin E in the skin by taking vitamin C and vitamin E as indexes, the vitamin E can form a reservoir on the skin due to strong lipid solubility, the skin reservoir effect of the embodiment 5 is better than that of the embodiment 11, and the liposome prepared by the liposome and the preparation containing the transdermal absorption enhancer are related. And vitamin E and vitamin C in the skin reservoir can better play the roles of antioxidation, whitening and anti-aging.

Similarly, the liposome gel of the cosmetic compositions according to examples 3 to 4 and 6 to 10 of the present invention also has the effect of promoting the permeation of the drug through the skin, and the effect on the skin depot is superior to that of example 11.

In addition, compared with the common gel prepared by only containing carbomer in the matrix, the gel matrix is prepared by adding the combination of carbomer, a pH regulator, a humectant and a transdermal absorption enhancer in the formula, and the prepared liposome gel greatly improves the permeation effect of skin and the effect of skin reservoir, so that the effects of resisting oxidation, whitening and resisting aging can be better exerted.

Effect example 1: skin irritation test of cosmetic compositions according to examples 3 to 10 of the present invention

(1) Skin irritation test in rabbits

Selecting 10 adult rabbits, unhairing, selecting 2 3 × 3cm blocks at two sides of the midline of the spine ² The whole skin was coated twice a day with 0.25g of liposome gel of the cosmetic composition of example 5 of the present invention or 0.25g of the third mixture (gel base) of the present invention twice per day to form a thin layer on the shaved skin, and the presence of erythema, redness, itching, erosion and pimple was observed and recorded after 24 hours of the application.

The results show that: after the skin is applied for 24 hours, skin irritation reactions such as erythema, redness, swelling, pruritus, erosion, pimple and the like are not seen, and the liposome gel of the cosmetic composition of the invention described in the embodiment 5 is safe and non-irritating to the skin.

Similarly, the results of comparative experiments in rabbit skin irritation experiments using the liposome gel formulations of the cosmetic compositions of the present invention described in examples 3 to 4 and examples 6 to 10 and the gel base show that: the liposome gel of each example after being applied for 24 hours has no skin irritation reaction such as erythema, redness, itching, erosion, pimple and the like on the rabbit skin, and the liposome gel of the cosmetic composition of the invention of the examples 3 to 4 and the examples 6 to 10 is safe and nonirritating to the skin.

(2) Human skin irritation test

The groups of 80 volunteers were divided into 8 groups (liposome gel of cosmetic composition according to examples 3 to 10 of the present invention), and 10 persons were observed by applying the liposome gel of each group to the inner side of the arm of each volunteer twice a day for 10 days.

The results show that: the application part of each volunteer does not turn red, eruption, blister and the like, and the liposome gel of the cosmetic composition of the embodiments 3 to 10 of the invention is further proved to have no irritation to the skin and good safety.

Effect example 2: animal pharmacodynamic experiments of the liposome gel of the cosmetic composition of the embodiments 3 to 10 of the invention on reducing wrinkle formation and delaying skin aging

Skin wrinkles are one of the common signs of aging in humans. The main component of skin tissue is collagen, and the content of hydroxyproline in the collagen and the content of other amino acids such as proline in the skin are closely related to skin aging.

Experimental animals: kunming mouse is provided by the laboratory animal center of Hubei university of traditional Chinese medicine.

The method and the result are as follows: taking 120 Kunming mice with half male and female, the weight of 18-22g, randomly dividing into 10 groups according to sex and weight, respectively: a blank control group (gel matrix group), a liposome gel group described in example 3 of the present invention, a liposome gel group described in example 4 of the present invention, a liposome gel group described in example 5 of the present invention, a liposome gel group described in example 6 of the present invention, a liposome gel group described in example 7 of the present invention, a liposome gel group described in example 8 of the present invention, a liposome gel group described in example 9 of the present invention, a liposome gel group described in example 10 of the present invention, and a comparative example gel experiment group. 1g of each group of samples is respectively and uniformly smeared on the back of each group of mice, the smearing area is 3cm multiplied by 3cm, the smearing is carried out once a day, and the smearing lasts for 21 days. 24 hours after the last application, the skin of the test area on the back of each group of mice was shaved off (to avoid interference of protein in the hair) and the area was cut, the tissue such as skin fat was removed, then the filter paper was rinsed with deionized water and wiped dry, the area was dried in an oven at 37 ℃ for 24 hours, then the area was cut and degreased with acetone for 48 hours, then the area was homogenized with a high speed homogenizer and dried in an oven at 37 ℃ for 24 hours, and the sample for measurement was obtained. 40mg of sample is taken, hydrochloric acid is used for hydrolysis at 110 ℃, evaporation is carried out in water bath at 80 ℃ for deacidification, 0.02mol/L hydrochloric acid is added for dissolution, and then the content of hydroxyproline and proline is measured by a Hitachi brand amino acid automatic analyzer. The results are shown in Table 1.

Table 1, effect of the groups on the content of hydroxyproline and proline in the skin of mice

Note: p < 0.01 compared to the blank control.

The experimental data of table 1 show that: the liposome gel groups described in embodiments 3 to 10 of the present invention can significantly increase the contents of hydroxyproline and proline in the skin of mice. The content of the amino acids in the skin of the mice of the experimental group using the gel of the comparative example was only slightly increased, and there was no significant difference from the blank control group. The above experimental results show that: the effect and efficacy of the liposome gel agents described in embodiments 3 to 10 of the present invention in the aspects of maintaining beauty and keeping young, reducing wrinkle formation, and delaying skin aging process are significantly better than those of the experimental gel agent group of the comparative example.

Effect example 3: the cosmetic composition of the invention is used for the effect test of beautifying and removing freckles

A female volunteer with black spots, chloasma, sunburn and the like on the face of 18-45 years old is selected to try out the cosmetic composition disclosed by the embodiment 8 of the invention, and the investigation result shows that the effective rate of removing the spots reaches over 95.0 percent, the effect is shown after 1 month of use, and the cosmetic composition disclosed by the invention is safe to use and has no irritation.

The cosmetic composition of example 8 of the present invention was continuously used by trial on human skin, in comparison with the formulation of the cosmetic composition of the comparative example of the present invention. The melanin content was determined using the MX18 instrument from CK, germany. The instrument is based on the principle of spectral absorption, and the content of melanin in the skin is determined by measuring the reflection quantity of light with specific wavelength irradiated on the skin of a human body. The emitter of the instrument probe emits light with three wavelengths of 568nm, 660nm and 880nm to the skin surface, and the receiver measures the light reflected from the skin. Because the quantity of reflected light is constant, the quantity of light absorbed by the skin can be measured, the content of melanin in the skin can be measured, and the higher the measured value is, the higher the content of melanin in the skin is. See in particular the results of table 2.

TABLE 2 comparison of the speckle removing effects

It can be seen from table 2 that the cosmetic composition of the present invention in example 8 can significantly reduce the melanin content in human skin, and the cosmetic composition of the present invention in example 8 can be used for removing speckle.

Similarly, the cosmetic compositions described in examples 3 to 7 and 9 to 10 of the present invention can also significantly reduce the melanin content in human skin, and the cosmetic compositions described in examples 3 to 7 and 9 to 10 of the present invention can be used for removing spots by beauty.

In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples and features of different embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction.

Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art within the scope of the present invention.

Claims

1. A cosmetic composition characterized in that it comprises: grape seed extract, white peony root extract, indian fruit oil, vitamin C ethyl ether and vitamin E;

it further comprises gel matrix, phospholipid, cholesterol, phosphate buffer solution and deionized water;

the gel matrix comprises: a humectant, carbomer, a transdermal absorption enhancer, and a pH adjusting agent;

the humectant is selected from: lentinan;

the pH regulator is selected from: triethanolamine;

the transdermal absorption enhancer is selected from: oat beta-glucan;

the cosmetic composition comprises the following components in parts by weight: 0.25 to 0.8 weight portion of grape seed extract, 0.25 to 0.8 weight portion of white peony root extract, 0.15 to 0.25 weight portion of Indian fruit oil, 0.4 to 0.8 weight portion of vitamin C ethyl ether, 0.15 to 0.45 weight portion of vitamin E, 9 to 15 weight portions of phospholipid, 1.8 to 3 weight portions of cholesterol, 0.6 to 1.2 weight portions of humectant, 1.6 to 2.4 weight portions of carbomer, 0.3 to 0.8 weight portion of transdermal absorption enhancer, 42 to 48 weight portions of phosphate buffer solution and a proper amount of pH regulator to regulate the pH value to be 6.1 to 6.9, and finally deionized water is added until the total weight portion of the prescription is 100 weight portions;

the composition is prepared into liposome gel for skin care;

the preparation method of the liposome gel comprises the following steps:

step (1): dissolving 4.5-7.5 parts by weight of phospholipid and 0.9-1.5 parts by weight of cholesterol in diethyl ether, removing the diethyl ether under reduced pressure, adding 0.4-0.8 part by weight of vitamin C ethyl ether and 0.25-0.8 part by weight of grape seed extract, dissolving the mixture in deionized water, performing rotary hydration for 15 minutes, performing ultrasonic treatment by using a probe type ultrasonic instrument in an ice bath environment at the power of 250W for 15 minutes, stopping the ultrasonic treatment for 2 seconds every 1 second, and fixing the volume to obtain a first liposome suspension which is a first mixture;

step (2): mixing 4.5-7.5 parts by weight of phospholipid, 0.9-1.5 parts by weight of cholesterol, 0.15-0.45 part by weight of vitamin E, 0.25-0.8 part by weight of white peony root extract and 0.15-0.25 part by weight of adducted fruit oil, dissolving in ethanol to form a mixed solution, quickly injecting the mixed solution into 42-48 parts by weight of phosphate buffer solution at the temperature of 60 ℃, removing the ethanol under reduced pressure, then performing ultrasound by using a probe type ultrasonic instrument, performing ultrasound for 15min at the power of 250W in an ice bath environment, stopping for 2 seconds every 1 second of ultrasound, and performing constant volume to obtain a second liposome suspension as a second mixture;

2. The liposomal gel of claim 1, wherein the phosphate buffer is formulated and formulatedThe preparation method comprises the following steps: 7.9g NaCl, 0.2g KCl and 0.24g KH were weighed out separately ₂ PO ₄ 、1.8g K ₂ HPO ₄ Dissolving in 800ml deionized water, adjusting the pH value of the solution to 7.4 by using HCl, finally adding deionized water to a constant volume of 1L to obtain a phosphate buffer solution, and storing the prepared solution in a refrigerator at 4 ℃.

3. The liposomal gel according to claim 1, wherein the phosphate buffer solution is formulated and formulated by: 7.9g NaCl, 0.2g KCl and 1.44g Na were weighed out separately ₂ HPO ₄ 、1.8g K ₂ HPO ₄ Dissolving in 800ml deionized water, adjusting the pH value of the solution to 7.4 by using HCl, finally adding deionized water to a constant volume of 1L to obtain a phosphate buffer solution, and storing the prepared solution in a refrigerator at 4 ℃.

4. The liposomal gel according to claim 1, wherein the phospholipid is natural phospholipid, semisynthetic phospholipid, fully synthetic phospholipid, a mixture of natural phospholipid and semisynthetic phospholipid, or a mixture of natural phospholipid and fully synthetic phospholipid, and the content of phosphatidylcholine is 50% to 99%.

5. The liposomal gel of claim 1, wherein the liposome gel is a gel,

comprises the following components in parts by weight: 0.5 part by weight of grape seed extract, 0.5 part by weight of white peony root extract, 0.2 part by weight of crabapple oil, 0.5 part by weight of vitamin C ethyl ether, 0.25 part by weight of vitamin E, 10 parts by weight of phospholipid, 2 parts by weight of cholesterol, 1 part by weight of lentinan, 2 parts by weight of carbomer, 0.5 part by weight of oat beta-glucan, 45 parts by weight of phosphate buffer solution and a proper amount of triethanolamine to adjust the pH value to be 6.6, and finally adding deionized water until the total weight of the formula is 100 parts by weight.

6. The liposomal gel of claim 1, wherein the liposome gel is a gel,

comprises the following components in parts by weight: 0.6 part by weight of grape seed extract, 0.6 part by weight of white peony root extract, 0.24 part by weight of crabapple oil, 0.6 part by weight of vitamin C ethyl ether, 0.3 part by weight of vitamin E, 12 parts by weight of phospholipid, 2.4 parts by weight of cholesterol, 0.9 part by weight of lentinan, 2.4 parts by weight of carbomer, 0.6 part by weight of oat beta-glucan, 45 parts by weight of phosphate buffer solution and a proper amount of triethanolamine to adjust the pH value to 6.2, and finally adding deionized water until the total weight of the formula is 100 parts by weight.

7. The liposomal gel according to claim 5, characterized in that the preparation method comprises the steps of:

step (1): dissolving 5g of phospholipid and 1g of cholesterol in 30mL of diethyl ether, decompressing in a water bath at 30 ℃ to remove the diethyl ether, then adding 0.5g of vitamin C ethyl ether and 0.5g of grape seed extract, dissolving with 20mL of deionized water, performing rotary hydration for 15 minutes, performing ultrasonic treatment for 15 minutes at 250W power in an ice bath environment by using a probe type ultrasonic instrument, stopping ultrasonic treatment for 2 seconds every 1 second, and finally performing constant volume to 25mL to obtain a first liposome suspension as a first mixture;

step (2): mixing 5g of phospholipid, 1g of cholesterol, 0.25g of vitamin E, 0.25g of white peony root extract and 0.2g of adducted fruit oil, dissolving in 10mL of ethanol to form a mixed solution, quickly injecting the mixed solution into a phosphate buffer solution with the temperature of 60 ℃ and the weight of 45g, removing the ethanol under reduced pressure, ultrasonically treating for 15min at the power of 250W by using a probe type ultrasonic instrument in an ice bath environment, stopping ultrasonic treatment for 2 seconds every 1 second, and finally fixing the volume to 50mL to obtain a second liposome suspension as a second mixture;

and (4): and (3) adding deionized water into the first mixture prepared in the step (1), the second mixture prepared in the step (2) and the third mixture prepared in the step (3) to make the total weight of the mixture be 100g, and fully mixing to obtain the skin-care liposome gel of the cosmetic composition.