CN107961174A - Lipstick containing stem cell secretion factors and preparation method thereof - Google Patents
Lipstick containing stem cell secretion factors and preparation method thereof Download PDFInfo
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- CN107961174A CN107961174A CN201711279684.3A CN201711279684A CN107961174A CN 107961174 A CN107961174 A CN 107961174A CN 201711279684 A CN201711279684 A CN 201711279684A CN 107961174 A CN107961174 A CN 107961174A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/927—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of insects, e.g. shellac
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/37—Esters of carboxylic acids
- A61K8/375—Esters of carboxylic acids the alcohol moiety containing more than one hydroxy group
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/4973—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom
- A61K8/498—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom having 6-membered rings or their condensed derivatives, e.g. coumarin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/63—Steroids; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/001—Preparations for care of the lips
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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Abstract
The invention relates to the technical field of skin care products, in particular to a lipstick containing stem cell secretion factors and a preparation method thereof, wherein the lipstick comprises the following raw materials in parts by weight as 100 parts by weight: 5-15 parts of natural beeswax, 5-15 parts of carnauba wax, 5-20 parts of coconut oil, 10-20 parts of jojoba oil, 5-20 parts of olive oil, 10-20 parts of shea butter, 5-10 parts of hydrogenated coconut oil glyceride, 5-10 parts of glyceryl caprylate, 1-10 parts of polyhydric alcohol, 1-10 parts of active peptide, 1-20 parts of asiaticoside, 1-10 parts of anthocyanin, 0.05-0.5 part of dry cell secretion factor and the balance of water. The lipstick provided by the invention is simple in raw material components, reasonable in formula and good in synergistic effect, can simultaneously give consideration to the dual effects of moisturizing and resisting wrinkles, and has a good effect of improving skin texture, namely delaying skin aging.
Description
Technical field
The present invention relates to skin care product technical field, and in particular to a kind of lipstick and its system containing stem cell secretion factor
Preparation Method.
Background technology
The skin of lip is more tender and lovely than face, self-protection and the poor ability for defending external injury.Ultraviolet is exactly pair
One of maximum foeign element of human skin injury.Ultraviolet irradiation can promote skin to produce a large amount of free radicals, cause skin to be sent out
The third contact of a total solar or lunar eclipse chemically reacts.Excessive ultraviolet irradiation, the lighter can make lip skin dehydration, and feeling in the summer of humid will not be too
Strongly.So people are easy to pigementation caused by ignoring ultraviolet institute energy and cheilogramma is deepened；Severe one can cause skin cancer.
At present, the mode of facial skin has only been paid attention to for the sun-proof of skin, people, sun-proof for tender and lovely skin of lip is not drawn
Rise and pay attention to, the lipstick used now does not possess sun-proof and Green Tea Extract effect substantially.
Lip composition by including oil and wax oil components and pigment composition form, oil components provide bating effect and
It is suitable to feel, and multicolour is presented in pigment.Be known as lip cuticula is significantly thinner than common skin and its day
Right moisturizing factor and water content are relatively low, so lip has several times higher than other skins of fluid loss characteristics.Since the skin of lip loses
Water is rapid, so under the environmental condition of such as dry weather, wrinkle is easily formed in lip skin histology and its tissue closed on
Line.
The content of the invention
In view of this, it is necessary to for it is above-mentioned the problem of, there is provided a kind of lipstick and its preparation containing stem cell secretion factor
Method, lipstick of the invention can play the role of promoting the physiological activity of lip skin to improve anti-wrinkle of lips effect.
The present invention provides a kind of lipstick containing stem cell secretion factor, using the mass fraction of the lipstick as 100, the lip
The raw material of cream is made of the component of following parts by weight：5-15 parts of natural beeswaxs, 5-15 parts of Brazil waxs, 5-20 portions of coconut oil,
10-20 parts of jojoba oils, 5-20 parts of olive oil, 10-20 parts of shea butters, 5-10 parts of hydrogenated coco-glycerides, 5-10 parts of glycerine
Caprylate, 1-10 part polyalcohol, 1-10 parts of active peptides, 1-10 parts of asiaticosids, 1-10 parts of anthocyanidin, 0.05-0.5 parts of stem cells
Secretion factor, and the water of surplus.
Preferably, the quality summation of the hydrogenated coco-glyceride and the glycerol caprylate is less than the coconut oil, suddenly
The quality summation of bar oil, olive oil and shea butter suddenly.
Preferably, the polyalcohol is selected from least one of glycerine, 1,3-BDO, dipropylene glycol or sorbierite.
Preferably, the active peptide be selected from palmitoyl oligopeptide, palmityl tetrapeptide -7, palmityl tripeptides -5, acetyl group tetrapeptide -
5th, at least two in acetyl group hexapeptide -7, Argireline or decarboxylation carnosine.
Preferably, the stem cell secretion factor is the secretion factor of umbilical cord mesenchymal stem cells.
Preferably, the stem cell secretion factor is 0.05-0.1 parts.
Preferably, the water is deionized water or pure water.
The present invention also provides a kind of preparation method of above-mentioned lipstick, the preparation method includes：5-15 parts by weight are natural
Beeswax, 5-15 parts by weight Brazil wax, 5-20 parts by weight coconut oil, 10-20 parts by weight jojoba oil, 5-20 parts by weight olives
Oil, 10-20 parts by weight shea butter, the parts by weight of hydrogenated coco glycerides of 5-10,5-10 parts by weight of glycerin caprylate form melting
Composition；1-10 parts of polyalcohols, 1-10 parts of active peptides, 1-10 parts of asiaticosids, 1-10 parts of anthocyanidin, 0.05-0.5 parts are done thin
The intracrine factor, and the water of surplus are mixed with the melt composition；Filling is into rod forming containers；It is cooled to a constant temperature
Degree, forms solid stick lipstick.
Preferably, the solid stick lipstick is can be from container with bar-shaped flexible solid bar.
Lipstick raw material components component provided by the invention is simple, is made of natural material, without essence, pigment and anti-corrosion
Agent, shelf-life up to 3 years, do not have human body after use any allergy and discomfort, long-time service can to skin as mild as a dove
Delay skin aging, improve lip skin, and gives healthy, soft and moist, plentiful lips.
Embodiment
In order to facilitate the understanding of those skilled in the art, the present invention is further retouched below in conjunction with specific embodiment
State.
The present invention provides a kind of lipstick containing stem cell secretion factor, using the mass fraction of the lipstick as 100, the lip
The raw material of cream is made of the component of following parts by weight：5-15 parts of natural beeswaxs, 5-15 parts of Brazil waxs, 5-20 portions of coconut oil,
10-20 parts of jojoba oils, 5-20 parts of olive oil, 10-20 parts of shea butters, 5-10 parts of hydrogenated coco-glycerides, 5-10 parts of glycerine
Caprylate, 1-10 part polyalcohol, 1-10 parts of active peptides, 1-10 parts of asiaticosids, 1-10 parts of anthocyanidin, 0.05-0.5 parts of stem cells
Secretion factor, and the water of surplus.The lipstick raw material components component obtained by each raw material of these weight ratios is simple, matches somebody with somebody
Fang Heli, cooperative effect is good, can take into account moisturizing and crease-resistant double effects well, and there is the preferable dermatoglyph that improves to delay
The effect of skin senescence.
In embodiment, active peptide is selected from palmitoyl oligopeptide, palmityl tetrapeptide -7, palmityl tripeptides -5, acetyl group
At least two in the acetyl group hexapeptide of tetrapeptide-5-7, Argireline or decarboxylation carnosine, palmityl tripeptides-5 belong to class signal
Win peptide, the generation of collagen and other extracellular matrix proteins (including elastin laminin and fibronectin etc.) can be promoted；
Palmityl tetrapeptide -7 can reduce the generation of interleukins (interleukin-6), diminish inflammation；Acetyl group tetrapeptide -5 is effective
Angiotensin-Converting (ACE) inhibitor, they can be Angiotensin II by suppressing angiotensin i-converting,
So as to improve blood circulation.It is used in combination by active peptide with other components, the water content of skin can be lifted, increase skin is tight
Solidity and reduction microgroove.Specifically, water is deionized water or pure water etc..
The present invention lipstick in, asiaticosid by with active peptide, stem cell secretion factor and polyalcohol combination,
So that comprising stable asiaticosid in the lipstick finally obtained, and good anti-wrinkle effect can be brought.
Natural beeswax used in the present invention is that four pairs of wax gland secretion shopping matter, its main component have below worker bee belly：Acid
Class, free fatty, free alkyl alcohol and carbohydrate.In addition, also carotenoid, vitamin A, aromatic substance etc., have
There is antibacterial and antisepsis.Jojoba oil has the function of good moist, softening skin quality, is easily merged with skin, there is superpower resist
Oxidation effectiveness；There is deep layer to moisten, effectively keep skin softness children sliding for shea butter, prevent season cracking, eliminating stimulates discomfort
Feel, while there is superpower antioxidant effect.Polyalcohol is in glycerine, 1,3-BDO, dipropylene glycol or sorbierite
At least one, the combined use of polyalcohol can strengthen the wetability of lipstick.Olive oil is rich in unrighted acid and various
Vitamin, is easily absorbed by the skin, salubrious naturally, absolutely not greasy feeling, moreover, Polyphenols Wheat Protein contained therein, energy
Phenomena such as color spot, wrinkle and there is certain sun-proof effect caused by the cell senescence for being effectively prevented from by oxidation being occurred by fat
Fruit.In preferred embodiment, the quality summation of hydrogenated coco-glyceride and the glycerol caprylate is less than the coconut oil, suddenly
The quality summation of bar oil, olive oil and shea butter suddenly.
Astaxanthin is a kind of very strong antioxidant, its singlet-oxygen quenching and the ability ratio β-Hu Luo for catching free radical
More than 10 times of Bu Su high, than then more than strong 100 times of vitamin E, inoxidizability is ascorbic more than 6000 times.Due to its natural antioxygen
Change activity, it can effectively prevent the light aging of skin, reduce injury, delaying cell aging, reductions of the UVA and UVB to skin
Melanin deposition, strengthens immunity of organisms；Astaxanthin has outstanding anti-inflammatory activity at the same time, for a variety of including cheilitis
Inflammation has good treatment and prevention effect.In addition, the distinctive color and luster of astaxanthin can allow the more aobvious elasticity of lips, tension force and profit
Pool sense.In the lipstick raw material of the present invention, it is used in combination by adding astaxanthin with other materials, lipstick wrinkle resistance can be being improved
While energy, prevention effect is played to cheilitis, and allow using the more aobvious moist sense of the lips after lipstick.
In embodiment, due to the acquisition of current mesenchymal stem cell, there are some, difficult and sources are unstable
Fixed, cell Proliferation does not have the umbilical cord mesenchymal stem cells speed fast；It is easy that although amnion mesenchymal stem cell obtains, cell volume
Greatly, growth rate is slow, obtains the of high cost of stem cell secretion factor；The most of donors of fat mesenchymal stem cell all exist itself
Inflammatory problems, inflammatory factor is also more in stem cell secretion factor, is unfavorable for being used for skin care item.The antifreeze hand cream of the present invention
In, stem cell secretion factor preferably uses the secretion factor of umbilical cord mesenchymal stem cells, and the stem cell secretion factor added contains
Measure as 0.05-0.1 parts.
Specifically, stem cell secretion factor freeze-dried powder preparation process is as follows：(1) healthy human body umbilical cord is collected, umbilical cord is made
Further cleaning, then cut with aseptic operation and cutting after umbilical cord removal outer membrane, vein and artery is divided into fine tissue.(2) shred
Fine tissue afterwards by add the pancreatin of debita spissitudo, clostridiopetidase A when digest umbilical cord tissue block 0.5 it is small when.To digest monokaryon
Cell, collects the cell for digesting and carries out adhere-wall culture with serum free medium, obtain umbilical cord mesenchymal stem cells.(3) filled between
The amplification cultivation of matter stem cell, the training of mescenchymal stem cell is carried out with stem cell media by way of convenient stem cells culture
Support, carried out changing liquid, fluid infusion according to cell growth condition.(4) cell growth status is observed, takes the positive mark of cell detection cell surface
The expression of will CD90, CD105 and cell surface feminine gender surface marker CD45, CD34.When height expresses above-mentioned detection material, take
50ml stem cell medias, are sub-packed in culture dish, and liquid level thickness is not higher than 3mm, covers preservative film, is put in -86 DEG C of refrigerators
Freeze 24h.(5) culture dish after freezing is taken out, some apertures is stabbed out on preservative film with fine needle, is positioned in freeze dryer, it is cold
It is lyophilized dry.(6) it is spare to be lyophilized into powdered packing.
Specifically, the present invention is used to prepare stem cell secretion factor using the cell in the 3rd generation to the 5th generation, it is used to prepare dry
The culture medium of cell-secretion factor is the culture supernatant that stem cell secretion factor is used to prepare without phenol red RPMI-1640
The culture supernatant of 72-96h time intervals after collection cell inoculation.
The present invention also provides a kind of preparation method of above-mentioned lipstick, preparation method includes：By pearl powder, vitamin E part, work
Property peptide, stem cell secretion factor and water mixed dissolution by weight, add poly- polysaccharide Firm agent and saualane/squalene, it is permanent
Tepidarium heating stirring, is cooled to room temperature, and the lipstick is obtained by ultramicronising.Wherein, the temperature of heating stirring is 40-65
DEG C, the speed of heating stirring turns/min for 3000-5000.Ultramicronising is real especially by dynamic high-pressure microjet ultramicronising technology
Existing, the dynamic high-pressure microjet ultramicronising technology includes shredding, crushes and homogeneous, the dynamic high-pressure microjet ultramicronising skill
The operating pressure of art is 140-180Mpa, and operating temperature is 20-30 DEG C.
Specific preparation method includes：By the natural beeswax of 5-15 parts by weight, 5-15 parts by weight Brazil wax, 5-20 weight
Part coconut oil, 10-20 parts by weight jojoba oil, 5-20 wt parts of olive oil, 10-20 parts by weight shea butter, 5-10 parts by weight
Hydrogenated coco-glyceride, 5-10 parts by weight of glycerin caprylate form melt composition；By 1-10 parts of polyalcohols, 1-10 parts of activity
Peptide, 1-10 part asiaticosid, 1-10 parts of anthocyanidin, 0.05-0.5 parts of stem cell secretion factors, and the water of surplus and melting group
Compound mixes；Filling is into rod forming containers；Certain temperature is cooled to, forms solid stick lipstick.Specifically, solid stick lip
Cream is can be from container with bar-shaped flexible solid bar.
Lipstick raw material components component provided by the invention is simple, is made of natural material, without essence, pigment and anti-corrosion
Agent, shelf-life up to 3 years, do not have human body after use any allergy and discomfort, long-time service can to skin as mild as a dove
Delay skin aging, improve lip skin, and gives healthy, soft and moist, plentiful lips.
It is further described below in conjunction with specific embodiment.
Embodiment 1
By the natural beeswax of 5 parts by weight, 10 parts by weight Brazil waxs, 10 parts by weight coconut oil, 20 parts by weight jojoba oils,
10 wt parts of olive oil, 20 parts by weight shea butters, 5 parts by weight of hydrogenated coco glycerides, 10 parts by weight of glycerin caprylates are formed
Melt composition；By 5 parts of polyalcohols, 2 parts of active peptides (1 part of palmitoyl oligopeptide and 1 part of palmityl tetrapeptide -7), 1 part of asiaticosid,
1 part of anthocyanidin, 0.05 part of stem cell secretion factor, and the water of surplus are mixed with melt composition；Fill to rod forming containers
In；Certain temperature is cooled to, forms solid stick lipstick S1.
Embodiment 2
By the natural beeswax of 10 parts by weight, 5 parts by weight Brazil waxs, 15 parts by weight coconut oil, 15 parts by weight jojoba oils,
15 wt parts of olive oil, 10 parts by weight shea butters, 10 parts by weight of hydrogenated coco glycerides, 5 parts by weight of glycerin caprylates are formed
Melt composition；By 10 parts of polyalcohols, 1 part of active peptide (0.5 part of palmityl tripeptides -5 and 0.5 part of acetyl group tetrapeptide -5), 1 part of product
Asiaticoside, 2 parts of anthocyanidin, 0.1 part of stem cell secretion factor, and the water of surplus are mixed with melt composition；Filling to rod into
Describe in device；Certain temperature is cooled to, forms solid stick lipstick S2.
Embodiment 3
By the natural beeswax of 8 parts by weight, 6 parts by weight Brazil waxs, 12 parts by weight coconut oil, 10 parts by weight jojoba oils,
12 wt parts of olive oil, 15 parts by weight shea butters, 8 parts by weight of hydrogenated coco glycerides, 8 parts by weight of glycerin caprylates form molten
Melt composition；By 1 part of polyalcohol, 10 parts of active peptides (- 7,2 parts of 2 parts of palmityl tripeptides -5,2 parts of palmityl tetrapeptides acetyl group four
Peptide -5,2 parts of acetyl group hexapeptides -7,2 parts of decarboxylation carnosines), 2 parts of asiaticosids, 5 parts of anthocyanidin, 0.2 part of stem cell secretion factor,
And the water of surplus is mixed with melt composition；Filling is into rod forming containers；Certain temperature is cooled to, forms solid stick lip
Cream S3.
Embodiment 4
By the natural beeswax of 6 parts by weight, 8 parts by weight Brazil waxs, 20 parts by weight coconut oil, 10 parts by weight jojoba oils,
10 wt parts of olive oil, 10 parts by weight shea butters, 5 parts by weight of hydrogenated coco glycerides, 5 parts by weight of glycerin caprylates form molten
Melt composition；By 5 parts of polyalcohols, 5 parts of active peptides (2 parts of Argirelines, 3 parts of decarboxylation carnosines), 10 parts of asiaticosids, 10 parts
Anthocyanidin, 0.5 part of stem cell secretion factor, and the water of surplus are mixed with melt composition；Filling is into rod forming containers；It is cold
But to certain temperature, solid stick lipstick S4 is formed.
Embodiment 5
By the natural beeswax of 10 parts by weight, 10 parts by weight Brazil waxs, 10 parts by weight coconut oil, 10 parts by weight Jojobas
Oil, 10 wt parts of olive oil, 10 parts by weight shea butters, 5 parts by weight of hydrogenated coco glycerides, 5 parts by weight of glycerin caprylate shapes
Into melt composition；By 8 parts of polyalcohols, 3 parts of active peptides (2 parts of palmitoyl oligopeptides and 1 part of decarboxylation carnosine), 5 parts of asiaticosids, 8
Part anthocyanidin, 0.3 part of stem cell secretion factor, and the water of surplus are mixed with melt composition；Filling is into rod forming containers；
Certain temperature is cooled to, forms solid stick lipstick S5.
Embodiment 6
By the natural beeswax of 7 parts by weight, 5 parts by weight Brazil waxs, 12 parts by weight coconut oil, 11 parts by weight jojoba oils,
11 wt parts of olive oil, 12 parts by weight shea butters, 6 parts by weight of hydrogenated coco glycerides, 9 parts by weight of glycerin caprylates form molten
Melt composition；By 10 parts of polyalcohols, 8 parts of active peptides (2 parts of palmitoyl oligopeptides, 3 parts of acetyl group tetrapeptides -5 and 3 parts of acetyl group hexapeptides -
8), 2 parts of asiaticosids, 3 parts of anthocyanidin, 0.08 part of stem cell secretion factor, and the water of surplus are mixed with the melt composition
Close；Filling is into rod forming containers；Certain temperature is cooled to, forms solid stick lipstick S6.
Embodiment 7
By the natural beeswax of 10 parts by weight, 10 parts by weight Brazil waxs, 5 parts by weight coconut oil, 10 parts by weight jojoba oils,
5 wt parts of olive oil, 10 parts by weight shea butters, 8 parts by weight of hydrogenated coco glycerides, 8 parts by weight of glycerin caprylates form molten
Melt composition；By 8 parts of polyalcohols, 6 parts of active peptides (- 7,1 parts of 2 parts of palmitoyl oligopeptides, 1 part of palmityl tetrapeptide acetyl group tetrapeptides -5
With 1 part of Argireline), 4 parts of asiaticosids, 7 parts of anthocyanidin, 0.06 part of stem cell secretion factor, and the water of surplus with
The melt composition mixing；Filling is into rod forming containers；Certain temperature is cooled to, forms solid stick lipstick S7.
Performance test：
1st, anti-oxidant test
The corresponding lipsticks of S1-S7 obtained by 0.5g embodiments are weighed, constant volume in 100mL volumetric flasks is transferred to after being dissolved with pure water,
Mass concentration is made as 5.00 × 10-3The sample solution of g/mL.
Compound concentration is 2 × 10 respectively-4The ethanol solution of the DPPH of g/mL, concentration are the salicylic nothings of 10mmol/L
The H that FeSO4 aqueous solutions that hydrous ethanol solution, concentration are 10mmol/L, concentration are 8.8mmol/L2O2Aqueous solution, pH are 8.2
Pyrogallol solution that HCl solution that Tris-HCl buffer solutions, concentration are 10mmol/L, concentration are 5mmol/L (using concentration as
The HCl solution of 10mmol/L is solvent).
(1) inhibiting rate of DPPH free radicals is tested
Testing sample solution 3mL is pipetted in 10mL volumetric flasks, it is 2 × 10 then to add 3mL concentration-4G/mL DPPH are molten
Liquid.Plug is covered, is shaken up, reacts 30min, absorbance A is measured at 517nmi；Measure 3mL sample liquids and 3mL alcohol mixeding liquids
Absorbance Aj；It is 2 × 10 to measure 3mL concentration-4G/mL DPPH solution and the mixed absorbance A of 3mL pure water0.According to formula P
=[1- (Ai- Aj)/A0] × 100%, the absorbance measured is brought into and tries to achieve inhibiting rate of the prepare liquid to DPPH free radicals.
(2) inhibiting rate of OH free radicals is tested
The salicylic acid solution of 10mmol/L and the FeSO of 10mmol/L are added into the volumetric flask of 10mL4Each 2mL of solution, so
Prepare liquid 2mL is added afterwards, adds the H that 2mL concentration is 8.8mmol/L2O2Solution, shakes up and is reacted after in 37 DEG C of water-baths
30min.Absorbance A is measured at 510nmi；H is replaced with 2mL pure water2O2Measure absorbance Aj；Prepare liquid is replaced with 2mL pure water
To measure absorbance A0.According to formula P=[1- (Ai- Aj)/A0] × 100%, the absorbance measured is brought into and tries to achieve prepare liquid
To the inhibiting rate of OH free radicals.
(3) to O2-The inhibiting rate test of free radical
The Tris-HCl buffer solutions 5mL that pH is 8.2 is taken to preheat 20min in 25 DEG C of water-baths, so in 10mL volumetric flasks
The pyrogallol solution 0.5mL of prepare liquid 2mL and 5mmol/L are sequentially added afterwards, are shaken up, are reacted in 25 DEG C of water-baths immediately
4min, measures its absorbance A at 320nm immediatelyi；Pyrogallol is replaced with the HCl solution 0.5mL of 10mmol/L, measure is inhaled
Luminosity Aj；Prepare liquid is replaced with 2mL pure water to measure absorbance A0.According to formula P=[1- (Ai- Aj)/A0] × 100%, will
The absorbance measured, which is brought into, tries to achieve prepare liquid to O2-The inhibiting rate of free radical.
Test result：
Inhibiting rate of the 1 embodiment lipstick solution of table to 3 kinds of free radicals
The corresponding lipstick solution of 1, table S1-S7 of the present invention is to three kinds of free radicals DPPH, OH, O2-Inhibiting rate, from
In table as can be seen that the present invention lipstick to three kinds of free radical inhibiting rate average levels all more than 60%, absolutely prove this hair
Bright lipstick has stronger oxidation resistance, can effectively remove free radical, and there is the preferable dermatoglyph that improves to delay
The effect of skin senescence.
2nd, lipstick skin irritation is tested
Using 30 women and 20 males as object, taken with adjusting spatula to rub on the stick lip balm of a diameter of 1.21cm
20mg lipstick samples, a sealing condition is created after fixed.Skin is pasted when 23 is small once, is opened 1 hour after patch, is then pressed
Checked according to the terminology of CTFA guides (1981).6 patch experiments have been carried out altogether, paste the 4th after skin
My god, in order to observe delayed reaction, carry out additional inspection.Data then utilize last time inspection result, compare.Comment
Price card is accurate as follows：
Without irritability：Without erythema and oedema
Somewhat irritability：Cause very slight erythema and oedema
Moderate irritability：Obvious erythema and oedema
3rd, the anti-wrinkle effect test of lip
The lipstick prepared according to S1-S7 is repeated to be applied to 100 20 to quadragenarian women in surrounding, then by making up
Teacher's evaluation uses the anti-wrinkle effect of the front/rear lip of lipstick, as follows：
The anti-wrinkle effect of lip or does not slightly change (0/100~97/3)
The anti-wrinkle effect of lip is slightly improved (96/4~86/14)
The anti-wrinkle effect of lip significantly improves (85/15~70/30)
Number of the anti-wrinkle effect of number/lip of the improved people of anti-wrinkle effect of lip without the people of change
4th, the evaluation of lipstick moistening effect
In S1-S7 by by all the components of the uniform dissolution in addition to component 12 and the anti-wrinkle of lips that is used for for preparing
Base composition prepares lipstick.Gained lipstick is applied to the inner arm of eight subjects, then after painting 3 it is small when by using skin table
Surface humidity tester measures the change of moisture-holding capacity over time, evaluates moistening effect.
Well：3 it is small when after, the coating time based on starting：Water holding capacity increase at least 10%
It is not poor：3 it is small when after, the coating time based on starting：Water holding capacity increase by 5~10%
It is poor：3 it is small when after, the coating time based on starting：Water holding capacity increase is less than 5%
2 embodiment lipstick properties test result of table
| Sample | Irritation | Anti-wrinkle effect | Moistening effect |
| Embodiment 1 | Without irritability | Significantly improve | Well |
| Embodiment 2 | Without irritability | Significantly improve | Well |
| Embodiment 3 | Without irritability | Significantly improve | Well |
| Embodiment 4 | Without irritability | Significantly improve | Well |
| Embodiment 5 | Without irritability | Significantly improve | Well |
| Embodiment 6 | Without irritability | Significantly improve | Well |
| Embodiment 7 | Without irritability | Significantly improve | Well |
Excellent anti-wrinkle of lips is presented from the lipstick for anti-wrinkle of lips in table 2, prepared in S1-S7 of the present invention to imitate
Fruit and excellent moistening effect, and safe and non-stimulating property.
Embodiment described above only expresses the several embodiments of the present invention, its description is more specific and detailed, but simultaneously
Therefore the limitation to the scope of the claims of the present invention cannot be interpreted as.It should be pointed out that for those of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the guarantor of the present invention
Protect scope.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Claims (9)
- A kind of 1. lipstick containing stem cell secretion factor, it is characterised in that using the mass fraction of the lipstick as 100, the lip The raw material of cream is made of the component of following parts by weight：5-15 parts of natural beeswaxs, 5-15 parts of Brazil waxs, 5-20 portions of coconut oil, 10-20 parts of jojoba oils, 5-20 parts of olive oil, 10-20 parts of shea butters, 5-10 parts of hydrogenated coco-glycerides, 5-10 parts of glycerine Caprylate, 1-10 part polyalcohol, 1-10 parts of active peptides, 1-20 parts of asiaticosids, 1-10 parts of anthocyanidin, 0.05-0.5 parts of stem cells Secretion factor, and the water of surplus.
- 2. lipstick according to claim 1, it is characterised in that the hydrogenated coco-glyceride and the glycerol caprylate Quality summation is less than the quality summation of the coconut oil, jojoba oil, olive oil and shea butter.
- 3. lipstick according to claim 1, it is characterised in that the polyalcohol is selected from glycerine, 1,3-BDO, double third At least one of glycol or sorbierite.
- 4. lipstick according to claim 1, it is characterised in that the active peptide be selected from palmitoyl oligopeptide, palmityl tetrapeptide- 7th, at least two in palmityl tripeptides -5, acetyl group tetrapeptide -5, acetyl group hexapeptide -7, Argireline or decarboxylation carnosine.
- 5. lipstick according to claim 1, it is characterised in that the stem cell secretion factor is umbilical cord mesenchymal stem cells Secretion factor.
- 6. lipstick according to claim 4, it is characterised in that the stem cell secretion factor is 0.05-0.1 parts.
- 7. lipstick according to claim 1, it is characterised in that the water is deionized water or pure water.
- 8. a kind of preparation method of lipstick according to claim 1, it is characterised in that the preparation method includes：By 5- The natural beeswax of 15 parts by weight, 5-15 parts by weight Brazil wax, 5-20 parts by weight coconut oil, 10-20 parts by weight jojoba oil, 5- 20 wt parts of olive oil, 10-20 parts by weight shea butter, the parts by weight of hydrogenated coco glycerides of 5-10,5-10 parts by weight of glycerin are pungent Acid esters forms melt composition；By 1-10 parts of polyalcohols, 1-10 parts of active peptides, 1-20 parts of asiaticosids, 1-10 parts of anthocyanidin, 0.05-0.5 parts of stem cell secretion factors, and the water of surplus are mixed with the melt composition；Filling is into rod forming containers； Certain temperature is cooled to, forms solid stick lipstick.
- 9. according to the preparation method described in right 8, it is characterised in that the solid stick lipstick is to be stretched from container with bar-shaped The solid bar of contracting.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711279684.3A CN107961174A (en) | 2017-12-06 | 2017-12-06 | Lipstick containing stem cell secretion factors and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711279684.3A CN107961174A (en) | 2017-12-06 | 2017-12-06 | Lipstick containing stem cell secretion factors and preparation method thereof |
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| Publication Number | Publication Date |
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| CN107961174A true CN107961174A (en) | 2018-04-27 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711279684.3A Withdrawn CN107961174A (en) | 2017-12-06 | 2017-12-06 | Lipstick containing stem cell secretion factors and preparation method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108498385A (en) * | 2018-04-28 | 2018-09-07 | 广州赛莱拉干细胞科技股份有限公司 | Composition containing Codium Tomentosum extract and its application in cosmetics |
| CN109280640A (en) * | 2018-09-30 | 2019-01-29 | 深圳市新仑生物科技有限公司 | A kind of preparation method of umbilical cord mesenchymal stem cells freeze-dried powder |
| CN110787088A (en) * | 2019-11-28 | 2020-02-14 | 广州那比昂生物科技有限公司 | Basic composition for lip wrinkle fading and preparation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102068391A (en) * | 2010-12-30 | 2011-05-25 | 广州赛莱拉化妆品科技有限公司 | Application of human steam cell growth factors in cosmetics |
| CN106727222A (en) * | 2016-12-09 | 2017-05-31 | 申艾平 | A kind of lipstick and preparation method thereof |
-
2017
- 2017-12-06 CN CN201711279684.3A patent/CN107961174A/en not_active Withdrawn
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102068391A (en) * | 2010-12-30 | 2011-05-25 | 广州赛莱拉化妆品科技有限公司 | Application of human steam cell growth factors in cosmetics |
| CN106727222A (en) * | 2016-12-09 | 2017-05-31 | 申艾平 | A kind of lipstick and preparation method thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108498385A (en) * | 2018-04-28 | 2018-09-07 | 广州赛莱拉干细胞科技股份有限公司 | Composition containing Codium Tomentosum extract and its application in cosmetics |
| CN109280640A (en) * | 2018-09-30 | 2019-01-29 | 深圳市新仑生物科技有限公司 | A kind of preparation method of umbilical cord mesenchymal stem cells freeze-dried powder |
| CN110787088A (en) * | 2019-11-28 | 2020-02-14 | 广州那比昂生物科技有限公司 | Basic composition for lip wrinkle fading and preparation method and application thereof |
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