CN115040446A

CN115040446A - SOD plant extract skin care composition, skin care product and preparation method thereof

Info

Publication number: CN115040446A
Application number: CN202210729603.XA
Authority: CN
Inventors: 曾会明
Original assignee: Individual
Current assignee: Individual
Priority date: 2022-06-24
Filing date: 2022-06-24
Publication date: 2022-09-13

Abstract

The invention discloses a SOD plant extract skin-care composition, a skin-care product and a preparation method thereof, wherein the SOD plant extract skin-care composition comprises SOD plant extract, meso-tetra (4-aminophenyl) porphyrin, copper chloride and a solvent; the SOD in the plant extract is connected with amino tetraphenyl porphyrin copper to form an oxygen atom-connected oxo bimetallic structure, the porphyrin ring provides a stable surrounding environment for active copper in the SOD, and the activity of Cu in the SOD can be enhanced through a connecting bond by virtue of the pi conjugation property of the porphyrin ring; meanwhile, the porphyrin copper serving as a photosensitizer has the sterilization effect, and the catalytic effect of the mimic enzyme structure can quickly decompose hydrogen peroxide generated by SOD disproportionation of superoxide ion free radicals into water, so that the skin care effect is enhanced by the porphyrin copper and the SOD. The skin care product can activate the muscle bottom, remove the turbid matter accumulated in the cells, resist the harm of free radicals and construct a skin defense system.

Description

SOD plant extract skin care composition, skin care product and preparation method thereof

Technical Field

The invention belongs to the field of skin care products, and particularly relates to a SOD plant extract skin care composition, a skin care product and a preparation method thereof.

Background

The skin is directly contacted with the external environment, and has an organ with the functions of protecting, excreting, sensing external stimulation and the like, so that when the skin is stimulated by the external environment or excreted in a barrier manner, some aging and damage, such as toxic byproducts of superoxide ion free radicals, inflammatory factors, deposited pigments and the like, exist in the skin. When the skin is serious, cell damage can be caused, and the quality of the skin is influenced, but the skin care product is the most direct method for eliminating the influence and improving the quality of the skin.

Superoxide dismutase SOD is a substance which is in the human body and corresponds to superoxide ion free radicals, has strong anti-inflammatory effect, can disproportionate the superoxide ion free radicals into hydrogen peroxide and oxygen which can be decomposed by catalase in the human body, realizes the elimination of the superoxide ion free radicals, and blocks the damage effect of the free radicals on the skin; on the other hand, human aging is a result of disorder in accumulation or elimination of reactive oxygen species free radicals, and thus excessive free radicals in the body cause cell damage and pigmentation. SOD can penetrate into skin cells rapidly, activate cell activity, and promote skin tissue regeneration, thereby improving skin resistance and self-repairing ability. SOD has good therapeutic effect on acne, pigmentation (such as acne, senile plaque, freckle, chloasma), eczema and dermatitis by scavenging oxygen free radicals, and can accelerate metabolism of pigmentation such as lipofuscin and mottle, activate muscle bottom, remove cell accumulation and turbid substance, resist free radical damage, and construct skin defense system.

However, SOD has strong reactivity, and how to keep stable performance when being externally used is a problem to be solved. In the prior art, SOD is coated by lipid substances to keep the stability and carry out slow release, but the lipid substances also prevent the SOD from contacting with the skin, so that the absorption rate is not high. Thus, higher requirements are imposed on the use of SOD.

Disclosure of Invention

In order to overcome the defects of the prior art, the first object of the present invention is to provide a skin care composition of SOD plant extract, wherein active Cu of SOD in the plant extract is connected with cytochrome P450 enzyme, so that not only is the external stability of SOD increased, but also the activity of Cu in SOD is enhanced through a connecting bond through pi conjugation property of porphyrin ring, and in addition, the catalytic effect of cytochrome P450 enzyme can rapidly decompose hydrogen peroxide generated by superoxide dismutase superoxide ion free radical of SOD into water, thereby cooperating with SOD to increase the skin care effect.

The second purpose of the invention is to provide a preparation method of the SOD plant extract skin care composition.

The third purpose of the invention is to provide a skin care product.

One of the purposes of the invention can be achieved by adopting the following technical scheme:

the SOD plant extract skin care composition comprises the following components in parts by weight: contains SOD plant extract 2.2-20 parts, meso-tetra (4-aminophenyl) porphyrin 0.05-0.3 part, copper chloride 0.02-0.1 part, and solvent 45-85 parts.

Further, the SOD-containing plant extract is one or a combination of more than two of a opuntia ficus-indica extract, a ampelopsis grossedentata extract, a chrysanthemum extract and a noni extract.

Further, the SOD-containing plant extract comprises the following components in parts by weight: 1-5 parts of opuntia ficus-indica extract, 1-5 parts of ampelopsis grossedentata extract and 0.2-5 parts of chrysanthemum extract.

Further, the SOD-containing plant extract comprises the following components in parts by weight: 1-5 parts of opuntia ficus-indica extract, 1-5 parts of ampelopsis grossedentata extract, 0.1-5 parts of chrysanthemum extract and 0.1-5 parts of noni extract.

Further, the solvent is a mixed solvent of ethanol and water, and the mass ratio of the ethanol to the water is 1: 3-10.

The second purpose of the invention can be achieved by adopting the following technical scheme:

a method for preparing SOD plant extract skin care composition,

stirring the SOD-containing plant extract, meso-tetra (4-aminophenyl) porphyrin, copper chloride and a solvent at 60-75 ℃ for 6-24h to obtain the SOD plant extract skin-care composition.

Further, oxygen-enriched air with the oxygen volume fraction of 50 percent is introduced into the solvent in the reaction process; the molar flow ratio of the introduced oxygen-enriched air to the copper chloride is 0.5-0.75: 1.

further, the reaction is carried out under a certain pressure, and the reaction pressure is 0.1-0.5 MPa.

The third purpose of the invention can be achieved by adopting the following technical scheme:

a skin care product contains the SOD plant extract skin care composition or the SOD plant extract skin care composition prepared by the preparation method.

Further, the skin care product is one of facial mask, cream and essence.

Compared with the prior art, the invention has the beneficial effects that:

1. the SOD plant extract skin care composition connects SOD in the plant extract with amino tetraphenyl porphyrin copper, the porphyrin ring provides stable surrounding environment for active copper in the SOD, and the activity of Cu in the SOD can be enhanced through a connecting bond through the pi conjugation property of the porphyrin ring; meanwhile, the porphyrin copper serving as a photosensitizer has the sterilization effect, and the catalytic effect of the mimic enzyme structure can quickly decompose hydrogen peroxide generated by SOD disproportionation of superoxide ion free radicals into water, so that the skin care effect is enhanced by the porphyrin copper and the SOD.

2. The preparation method of the SOD plant extract skin care composition can enable SOD and amino tetraphenyl porphyrin copper to form an oxo bimetallic structure with connected oxygen atoms, and the porphyrin ring of the porphyrin copper plays a role in stabilizing the surrounding environment of the SOD active Cu center.

3. The skin care product contains the SOD plant extract skin care composition, can activate the muscle bottom, eliminate the turbid substances accumulated by cells, resist the harm of free radicals and construct a skin defense system.

Drawings

FIG. 1 is a UV spectrum of a mixture of comparative example 1;

FIG. 2 is a UV spectrum of the mixture of example 4;

FIG. 3 illustrates the binding of SOD to meso-tetrakis (4-aminophenyl) porphyrin copper.

Detailed Description

The technical solution of the present invention will be clearly and completely described below with reference to the specific embodiments. It is to be understood that the described embodiments are merely some, and not all, embodiments of the invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.

In order to ensure that the SOD component in the plant extract can be stably absorbed in the external application process and make full use of the active component, the invention provides the SOD plant extract skin-care composition, which achieves the aim and achieves the effect of synergistically improving the skin quality.

SOD can be extracted from different plants, and the extraction method is not repeated here; the invention aims to fully utilize SOD in plant extracts. It is known that the active center of Cu/Zn-SOD includes one Cu and one Zn ion. And the existence of Cu is necessary for the activity of Cu/Zn-SOD, and the Cu directly reacts with superoxide anion free radicals to generate disproportionation reaction. The divalent copper ion is coordinately bound to the nitrogen atoms of the four surrounding histidines in a distorted, near-planar square configuration. Therefore, Cu can be connected from both surface directions of the square.

Therefore, in the process of forming meso-tetra (4-aminophenyl) porphyrin copper by using meso-tetra (4-aminophenyl) porphyrin and copper chloride, dimerization occurs between Cu in the SOD and Cu in the porphyrin copper in the presence of oxygen, and the Cu in the SOD and the Cu in the porphyrin copper are connected through oxygen atoms to form an oxo bimetallic structure, so that the surrounding environment of the Cu active center in the SOD is stabilized by using steric hindrance of a porphyrin ring. And the pi conjugation property of the porphyrin ring enhances the activity of Cu in the SOD through a connecting bond.

In one embodiment, the SOD-containing plant extract is one or more of Opuntia ficus-indica extract, Ampelopsis grossedentata extract, Chrysanthemum flower extract, and Morinda citrifolia extract.

The above plant extracts contain SOD active components, and wherein Opuntia Ficus lndica extract is an inducer of hyaluronic acid synthesis and collagen synthesis, and can effectively inhibit interleukin-1 b synthesis, thus having moisturizing, anti-inflammatory, anti-aging, and skin appearance, texture and moisture improving effects. The Opuntia ficus-indica extract is obtained by extracting stems, leaves, roots, fruits, husks and the like of Opuntia ficus-indica plants.

Ampelopsis grossedentata is a common medicinal material for minority people in Yao, Dong, Tujia and the like in China. The Ampelopsis grossedentata extract is rich in flavonoids, polyphenols and the like, and has antioxidant and anti-inflammatory effects; the whitening and freckle-removing facial mask has the effects of clearing various free radicals and delaying skin aging, and can reduce melanin generation by inhibiting the activity of the tyrosinase, so that the whitening and freckle-removing effects are achieved.

Taking stems and leaves of Ampelopsis grossedentata (Ampelopsis grossedentata) of Ampelopsis as raw materials, extracting with solvent, filtering, and spray drying to obtain Ampelopsis grossedentata extract; wherein the solvent is water, ethanol or ethanol water solution; the ratio of material to liquid is 1: 10-30 parts of; the extraction temperature is 40-100 ℃, and the extraction time is 1-h.

The flos Chrysanthemi extract is prepared from dried capitula of flos Chrysanthemi of Compositae, and contains flavonoids and phenols as main active ingredients. The chrysanthemum extract chrysanthemum can be obtained by ultrasonic extraction: ultrasonically treating dry capitula of flos Chrysanthemi in 5-10 times of 50-80% ethanol solution at 25-40 deg.C for 30-120min, extracting for 2-3 times, concentrating the extractive solution, and vacuum drying to obtain flos Chrysanthemi extract.

The noni extract has important effects in resisting aging, radiation and inflammation. The Morinda citrifolia extract is rich in free radical scavenging substances such as phenols, organic acids and alkaloids. The free radical scavenging substances include polyphenol, flavone, polysaccharide, procyanidin, squalene, vitamin C, vitamin E and carotene. Noni has strong antibacterial and bactericidal effects, and anthraquinone in polyphenol can effectively inhibit infection of various bacteria, and can relieve pain caused by inflammation. Noni fruit treats urticaria, an allergic skin reaction, by inhibiting histamine at the cellular level. It can even be used to treat certain skin conditions such as acne, herpes, burns and topical sunburn. Noni is effective in reducing blood glucose levels in the wound area and thus promoting rapid healing of the wound. Noni helps repair and regenerate cells, and rebuilds healthy new cells. The silolin in the phenolic substances has the function of repairing cells and improves the self-healing capacity of the silolin. The preparation method of the noni extract comprises ultrasonic extracting noni powder in water solution, vacuum filtering, and concentrating to obtain concentrated solution; adding anhydrous ethanol into the concentrated solution, precipitating with ethanol, centrifuging to remove supernatant to obtain Morinda citrifolia extract.

Therefore, the SOD active substance in the plant extract can be matched with other components in the extract to enhance the skin care effect.

In one embodiment, the SOD-containing plant extract comprises the following components in parts by weight: 1-5 parts of opuntia ficus-indica extract, 1-5 parts of ampelopsis grossedentata extract and 0.2-5 parts of chrysanthemum extract.

In one embodiment, the SOD-containing plant extract comprises the following components in parts by weight: 1-5 parts of opuntia ficus-indica extract, 1-5 parts of ampelopsis grossedentata extract, 0.1-5 parts of chrysanthemum extract and 0.1-5 parts of noni extract.

In one embodiment, the solvent is a mixed solvent of ethanol and water, and the mass ratio of the ethanol to the water is 1: 3-10. The mixed solvent of ethanol and water can make the components in the plant extract better dissolved.

The invention also provides a preparation method of the SOD plant extract skin-care composition,

In one embodiment, oxygen-enriched air with the oxygen volume fraction of 50 percent is introduced into the solvent during the reaction process; the molar flow ratio of the introduced oxygen-enriched air to the copper chloride is 0.5-0.75: 1.

in one embodiment, the reaction is carried out at a pressure of from 0.1 to 0.5 MPa.

The SOD-containing plant extract, meso-tetra (4-aminophenyl) porphyrin and copper chloride are heated and reacted in a solution, wherein the meso-tetra (4-aminophenyl) porphyrin and Cu form an oxo bimetallic structure connected with active Cu in SOD in the process of forming meso-tetra (4-aminophenyl) porphyrin copper, so that a porphyrin ring can play a role in stabilizing SOD, and the activity of Cu in SOD can be enhanced through a connecting bond through the pi conjugation property of the porphyrin ring. Meanwhile, the porphyrin copper serving as a photosensitizer has the sterilization effect, and the catalytic effect of the mimic enzyme structure can quickly decompose hydrogen peroxide generated by SOD disproportionation of superoxide ion free radicals into water, so that the skin care effect is enhanced by the porphyrin copper and the SOD.

The invention also provides a skin care product which contains the SOD plant extract skin care composition or the SOD plant extract skin care composition prepared by the preparation method.

In one embodiment, the skin care product is one of a mask, a cream and a essence.

Example 1:

SOD plant extract skin care composition

1 part of opuntia ficus-indica extract, 1 part of ampelopsis grossedentata extract and 5 parts of chrysanthemum extract; 0.05 part of meso-tetra (4-aminophenyl) porphyrin and 0.02 part of copper chloride are added into 4 parts of ethanol and 40 parts of water, oxygen-enriched air with the oxygen volume fraction of 50% is introduced under 0.1MPa, and the molar flow ratio of the oxygen-enriched air to the copper chloride is 0.5: 1; reacting at 75 deg.C for 24 hr, and cooling to room temperature to obtain SOD plant extract skin care composition.

Example 2:

SOD plant extract skin care composition

5 parts of Opuntia ficus-indica extract, 3 parts of Ampelopsis grossedentata extract and 0.1 part of chrysanthemum extract; 0.18 part of meso-tetra (4-aminophenyl) porphyrin and 0.05 part of copper chloride are added into 9 parts of ethanol and 63 parts of water, oxygen-enriched air with the oxygen volume fraction of 50% is introduced under 0.3MPa, and the molar flow ratio of the oxygen-enriched air to the copper chloride is 0.6: 1; reacting at 70 deg.C for 16h, and cooling to room temperature to obtain SOD plant extract skin care composition.

Example 3:

SOD plant extract skin care composition

3 parts of Opuntia ficus-indica extract, 5 parts of Ampelopsis grossedentata extract, 2.5 parts of chrysanthemum extract and 0.1 part of noni extract; 0.23 part of meso-tetra (4-aminophenyl) porphyrin and 0.08 part of copper chloride are added into 15 parts of ethanol and 45 parts of water, oxygen-enriched air with the oxygen volume fraction of 50% is introduced under 0.2MPa, and the molar flow ratio of the oxygen-enriched air to the copper chloride is 0.75: 1; reacting at 65 deg.C for 12 hr, and cooling to room temperature to obtain SOD plant extract skin care composition.

Example 4:

SOD plant extract skin care composition

3 parts of Opuntia ficus-indica extract, 3 parts of Ampelopsis grossedentata extract, 3 parts of chrysanthemum extract and 2.5 parts of noni extract; 0.25 part of meso-tetra (4-aminophenyl) porphyrin and 0.08 part of copper chloride are added into 9 parts of ethanol and 56 parts of water, oxygen-enriched air with the oxygen volume fraction of 50 percent is introduced under 0.3MPa, and the molar flow ratio of the oxygen-enriched air to the copper chloride is 0.65: 1; reacting at 60 deg.C for 15 hr, and cooling to room temperature to obtain SOD plant extract skin care composition.

Example 5:

SOD plant extract skin care composition

3 parts of opuntia ficus-indica extract, 3 parts of ampelopsis grossedentata extract, 5 parts of chrysanthemum extract and 5 parts of noni extract; 0.3 part of meso-tetra (4-aminophenyl) porphyrin and 0.1 part of copper chloride are added into 11 parts of ethanol and 44 parts of water, oxygen-enriched air with the oxygen volume fraction of 50 percent is introduced under 0.3MPa, and the molar flow ratio of the oxygen-enriched air to the copper chloride is 0.65: 1; reacting at 65 deg.C for 12 hr, and cooling to room temperature to obtain SOD plant extract skin care composition.

Comparative example 1

3 parts of Opuntia ficus-indica extract, 3 parts of Ampelopsis grossedentata extract, 3 parts of chrysanthemum extract and 2.5 parts of noni extract; 0.25 part of meso-tetrakis (4-aminophenyl) porphyrin and 0.08 part of copper chloride are added into 9 parts of ethanol and 56 parts of water, and the mixture is stirred for 15 hours at room temperature to obtain a mixture.

Comparative example 2

1 part of opuntia ficus-indica extract, 1 part of ampelopsis grossedentata extract and 5 parts of chrysanthemum extract; 0.05 part of meso-tetra (4-aminophenyl) porphyrin and 0.02 part of copper chloride are added into 4 parts of ethanol and 40 parts of water, oxygen-enriched air with the oxygen volume fraction of 50% is introduced under 0.1MPa, and the molar flow ratio of the oxygen-enriched air to the copper chloride is 0.5: 1; reacting at 40 ℃ for 24h, and cooling to room temperature to obtain a mixture after the reaction is finished.

Comparative example 3

Adding 0.25 part of meso-tetra (4-aminophenyl) porphyrin and 0.08 part of copper chloride into 9 parts of ethanol and 56 parts of water, introducing oxygen-enriched air with the oxygen volume fraction of 50% under 0.3MPa, wherein the molar flow ratio of the oxygen-enriched air to the copper chloride is 0.65: 1; reacting at 60 ℃ for 15h, and cooling to room temperature to obtain a mixture after the reaction is finished.

Comparative example 4

3 parts of Opuntia ficus-indica extract, 3 parts of Ampelopsis grossedentata extract, 3 parts of chrysanthemum extract and 2.5 parts of noni extract; adding into 9 parts of ethanol and 56 parts of water, and stirring at room temperature to obtain the SOD plant extract skin care composition.

Comparative example 5

The difference from example 4 is that 0.08 part of copper chloride is not added, and the other composition and preparation method are the same.

Test example 1

Human skin patch test

Testing an instrument: basic equipment and reagents, a spot tester, a low-sensitivity adhesive tape and a marking pen;

the human skin patch test (refer to technical Specification for cosmetic safety (2015 version)) spot-patch test method is used for testing, 240 tested persons meeting the standard are selected and averagely divided into 6 groups of 40 persons; corresponding to 5 groups, 0.020-0.025ml of the SOD plant extract skin care composition samples of examples 1-5 were applied to a chamber of a spot tester, blank group 1, given the same amount of water, applied to the back or forearm curvature of the subject with a special adhesive tape for external use, after 24h the spot tester was removed, the skin reactions were observed 30min, 6h, and 24h after removing the spot tester from the test object, and the results were recorded according to the skin reaction grading standards as shown below; the rating scale is shown in Table 1 and the results are shown in Table 2.

TABLE 1 skin closed Patch test skin response grading Standard

TABLE 2 Patch adverse reaction results

Through the closed patch test, after the subjects use the SOD plant extract skin care composition of example 1-5 for 24 hours, no adverse reaction is shown, and no symptoms such as erythema, burning sensation, pruritus and the like appear, and the negative reaction is shown, which indicates that the SOD plant extract skin care composition of example 1-5 has no irritation to skin.

Test for antibacterial Properties

The diameters of the inhibition zones of examples 1 to 5 and comparative examples 1 to 5 on staphylococcus aureus, propionibacterium acnes, escherichia coli and aspergillus niger were measured respectively, the larger the diameter of the inhibition zone is, the better the antibacterial effect is, and the test results are shown in table 3:

TABLE 3 results of zone of inhibition

As can be seen from Table 3, the inhibition zones of examples 1-5 of the present invention against Staphylococcus aureus, Propionibacterium acnes, Escherichia coli, and Aspergillus niger are all greater than the comparative examples, indicating that the SOD plant extract skin care composition prepared by the present invention has better antibacterial performance. Comparative example 4 and comparative example 4 have equivalent effects, and comparative example 3 has good effects, which shows that the porphyrin ring has the metal Cu to play the antibacterial effect; thus, comparative examples 1-2 are better than comparative examples 3-4 but are inferior to examples 1-5, which show that the plant extract and copper porphyrin can synergistically improve the bacteriostatic effect after being mixed, but the bacteriostatic effect is better after SOD in the extract is combined with copper porphyrin.

Superoxide anion radical scavenging test

Evaluation of scavenging Properties of superoxide anion free radicals by Phthalic triphenol autoxidation

Determination of superoxide anion scavenging ability: the superoxide anion scavenging ability is measured by pyrogallol autoxidation. 1mL of each of the samples of examples 1 to 5 and 1 to 4 in the ratio was taken, 4.5mL of 50mmol/L Tris-HCl (Tris: Tris-hydroxymethyl) buffer solution having a pH of 8.2 and 3.2mL of distilled water were added, the mixture was mixed, the mixture was incubated at 27 ℃ for 10min, 0.3mL of a preheated 3mmol/L pyrogallol solution at 25 ℃ was immediately added after the mixture was taken out, the absorbance was measured at intervals of 30s at 320nm using distilled water as a reference solution for 5min, and the increase in absorbance per minute in the linear range was calculated. The blank control solution was replaced with 1mL of distilled water. The clearance was calculated according to the formula:

clearance (%) (. DELTA.Ae. DELTA.A)/. DELTA.Ae

Wherein Ae is the increase value of absorbance per minute of the blank control group; as is the increase in absorbance per minute for the sample set. The results are shown in Table 4:

TABLE 4 superoxide anion radical scavenging ability

As can be seen from Table 1, examples 1 to 5 exhibited better superoxide anion radical scavenging ability than comparative examples 1 to 2 and comparative example 5, indicating that Cu in SOD and copper atom in copper porphyrin were linked through oxygen atom, pi conjugation property of porphyrin ring enhanced the activity of Cu in SOD through the linking bond. Comparative example 3 shows that porphyrin copper has poor scavenging ability for superoxide anion free radicals, and comparative example 4 shows that the composition of opuntia ficus-indica extract, vitis vinifera extract, chrysanthemum extract and morinda citrifolia extract has good scavenging ability for superoxide anion free radicals, so that the scavenging ability for superoxide anion free radicals is enhanced after the combination of SOD and porphyrin copper in examples 1-5.

Test example 2

10ml of each of the mixture of comparative example 1 and the SOD plant extract skin care composition of example 4 were taken, vacuum-dried to 4ml, and then scanned at a high speed between 200 and 750nm wavelength in a UV-2550 type UV-visible spectrophotometer (Shimadzu, Japan) with the UV spectrum of comparative example 1 as shown in FIG. 1 and the UV spectrum of example 2 as shown in FIG. 2.

As can be seen from FIG. 1, there are five characteristic peaks between 400-700, wherein the peak around 420nm is the Soret band peak of meso-tetrakis (4-aminophenyl) porphyrin, and the four peaks between 500-700nm are the Q band peaks, which are characteristic peaks of a standard meso-tetrakis (4-aminophenyl) porphyrin; indicating that meso-tetrakis (4-aminophenyl) porphyrin was not bound to a demetallized atom in the product of comparative example 1. In FIG. 1, there is a powder at 270nm, which is the characteristic peak generated by SOD absorption at 250-270 nm. Therefore, in FIG. 1, SOD and meso-tetrakis (4-aminophenyl) porphyrin as well as copper chloride did not react.

In FIG. 2, it can be clearly seen that the four peaks between 500-700nm in FIG. 1 have been reduced to 2 in FIG. 2, which is a typical change after the metal ion has replaced the H atom of the porphyrin center to form a porphyrin metal, indicating that the Cu ion has been attached to the middle of the porphyrin ring. And a large powder appears at the position between 270nm and 400nm, which is generally considered to be generated after bridging occurs, and the absorption peak of the combined SOD moves to about 230nm, so that the porphyrin metal and the SOD form a bridging bridge to form an oxo bimetallic substance shown in figure 3, and the dissolution bacteriostasis effect and the superoxide anion free radical scavenging capacity of the composition in example 4 are also known to be changed relative to the composition in comparative example 1.

In summary, according to the skin care composition containing the SOD plant extract, SOD in the plant extract is connected with amino tetraphenylporphyrin copper to form an oxygen atom-connected oxo bimetallic structure, a porphyrin ring provides a stable surrounding environment for active copper in the SOD, and the activity of Cu in the SOD can be enhanced through a connecting bond through the pi conjugation property of the porphyrin ring; meanwhile, the porphyrin copper serving as a photosensitizer has the sterilization effect, and the catalytic effect of the mimic enzyme structure can quickly decompose hydrogen peroxide generated by SOD disproportionation of superoxide ion free radicals into water, so that the skin care effect is enhanced by the porphyrin copper and the SOD. The skin care product contains the SOD plant extract skin care composition, can activate the muscle bottom, eliminate the turbid substances accumulated by cells, resist the harm of free radicals and construct a skin defense system.

The above embodiments are only preferred embodiments of the present invention, and the protection scope of the present invention is not limited thereby, and any insubstantial changes and substitutions made by those skilled in the art based on the present invention are within the protection scope of the present invention.

Claims

1. The SOD plant extract skin care composition is characterized by comprising the following components in parts by weight: contains SOD plant extract 2.2-20 parts, meso-tetra (4-aminophenyl) porphyrin 0.05-0.3 part, copper chloride 0.02-0.1 part, and solvent 45-85 parts.

2. The SOD plant extract skin care composition as claimed in claim 1, wherein the SOD plant extract is one or more of Opuntia ficus-indica extract, Ampelopsis grossedentata extract, Chrysanthemum extract, and Morinda citrifolia extract.

3. The SOD plant extract skin care composition as claimed in claim 2, wherein the SOD plant extract comprises the following components in parts by weight: 1-5 parts of opuntia ficus-indica extract, 1-5 parts of ampelopsis grossedentata extract and 0.2-5 parts of chrysanthemum extract.

4. The SOD plant extract skin care composition as claimed in claim 2, wherein the SOD plant extract comprises the following components in parts by weight: 1-5 parts of opuntia ficus-indica extract, 1-5 parts of ampelopsis grossedentata extract, 0.1-5 parts of chrysanthemum extract and 0.1-5 parts of noni extract.

5. The SOD plant extract skin care composition as claimed in claim 1, wherein the solvent is a mixed solvent of ethanol and water, and the mass ratio of ethanol to water is 1: 3-10.

6. A method for preparing the SOD plant extract skin care composition as defined in any one of claims 1-5,

7. The method for preparing the SOD plant extract skin care composition according to claim 5,

oxygen-enriched air with the oxygen volume fraction of 50 percent is introduced into the solvent in the reaction process; the molar flow ratio of the introduced oxygen-enriched air to the copper chloride is 0.5-0.75: 1.

8. the method for preparing the SOD plant extract skin care composition according to claim 4,

the reaction is carried out under a certain pressure, and the reaction pressure is 0.1-0.5 MPa.

9. A skin care product comprising the SOD plant extract skin care composition according to any one of claims 1 to 5 or the SOD plant extract skin care composition prepared by the preparation method according to any one of claims 6 to 8.

10. The skin care product according to claim 9, wherein the skin care product is one of a mask, a cream and a serum.