KR101827601B1 - Cosmetic composition containing the extracts of moringa oleifera and morus having anti-oxidation and anti-inflammatory - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising a Moringa leaf extract and a leaf extract as an active ingredient.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cosmetic composition containing an extract of Moringa leaves and upper leaves having antioxidant and anti-inflammatory activity,

The present invention relates to a functional cosmetic composition having an antioxidant and anti-inflammatory effect containing, as an active ingredient, a mixed extract obtained from Moringa oleifera and Morus. More specifically, it is possible to prepare an extract of Moringa leaf or Upper leaf, or to prepare an extract using Supercritical Extraction method, so that a Moringa leaf or a Moringa leaf containing various kinds and a large amount of efficacy components, The present invention relates to a cosmetic composition capable of exhibiting higher efficacy by providing a leaf extract and using it.

Moringa is a tree with a height of 5 ~ 10m and it is cultivated in Asia, Africa and Arabia. It is rich in proteins and vitamins, has a high nutritional value, and medically has various pharmacological actions such as hyperglycemia, anti-inflammation and anti-cancer. Moringa flowers, roots, seeds, leaves and fruits have vitamins, flavonoids, amino acids Are known to be rich in phytochemicals (Bharali R, et al.). Among the various parts of Moringa, leaves are rich in β-carotene, protein, vitamin C, and calcium, and are used as antioxidants and have been reported to be effective in skin inflammation and skin wound healing (Harnza AA, et al.). In addition, the extract of Moringa contains physiologically active substances such as flavonoids, isothiocyanates, glucosinolates, and thiocarbarnates, and the superiority of Moringa leaves has been shown in reports of protection of liver function, inhibition of cell proliferation and inhibition of cell proliferation Mahajan SG, et al.).

The leaf has been used for silkworms since ancient times, but recently it has been used as a car as a functional food material since various functions are known. In one room, it eliminated each species and species, benefited the small intestine and colon, removed gout, relieved thirst and used it to suppress aging. The upper leaves are rich in protein, vitamins, minerals and dietary fiber, and contain various physiologically active substances such as flavone, steroid and triterpene. The antioxidant activity of aqueous extracts of the upper leaves and the various physiological activities using mice were studied in the effects of leaf extracts on serum lipids in rats and healthy adults (Kim S.Y., et al.). Recently, there is a considerable amount of antimicrobial substances among natural plants such as herbal medicines, and studies on pharmacological actions and natural antimicrobial substances of these components are actively conducted. In particular, the top leaf contains the flavonoid components rutin, quercetin, isoquercetin and moracetin. These flavonoids have been reported to act as free radical scavengers (Kazuhisa M., et al.), Which directly react with reactive oxygen species and remove them.

Meanwhile, due to recent advances in science and medicine, there has been a growing interest in functional cosmetics that can prevent skin aging as they enter the aging society worldwide. Skin aging can be roughly divided into two types, one is the aging phenomenon that can not be avoided as the years pass through as an intrinsic aging, and the second is the aging process, which is the photoaging of the face exposed to sunlight for a long time, Which is caused by the combination of the effects of endogenous aging and ultraviolet rays.

There are many known theories of aging, but the most widely accepted aging theory is the free radical theory. Reactive Oxygen Species (ROS) in the surrounding environment and the human body are physiological actions such as respiration, The toxic substance is produced and extinguished constantly, and it exists in 3 ~ 5% in normal condition. These reactive oxygen species may be present in the form of free radicals such as superoxide radicals or hydroxyl radicals or in the form of free radicals such as compounds with paired electrons such as hydrogen peroxide or singlet oxygen .

Active oxygen has the advantage of biologically protecting bacteria in the physiological system, but generally causes oxidation in vivo, causing harmful effects that cause disease. It is also reported that these reactive oxygen species attack biological molecules, damaging cells and tissues, and causing various diseases related to aging and diseases of various adult diseases.

Research on antioxidants capable of inhibiting oxidation by active oxygen has been actively carried out in the beauty industry, and the research and development of natural materials and substances having strong antioxidant power are attracting attention as anti-aging substances.

Korean Patent Laid-Open No. 10-2013-0088224 discloses a patent for a composition for external application for skin containing an extract of Moringa seed, Korean Patent Laid-Open No. 10-2012-0108916 discloses a composition containing triglyceride, fatty acid and polar It is shown that the extract of Moringa seed is suitable as a dermatological composition as a patent for a seed seed extract and a cosmetic and dermatological composition of a moringa seed containing lipid-containing oil and polyphenol.

Korean Patent No. 10-1072832 or Korean Patent Laid-open No. 10-2008-0078371 discloses a patent relating to an atopic dermatitis inhibiting composition or a skin whitening cosmetic raw material containing an extract of Upper Lobster as an active ingredient, It has been shown that the extracts of mulberry leaves inhibit atopic dermatitis and help skin whitening.

On the other hand, vitamin C, which is known as a typical antioxidant, is a typical water-soluble vitamin. It plays a role as metabolism and catalyst in the body, and is an essential ingredient of collagen synthesis in human skin. It is used for anti-aging, It is well known that it is very effective in improving pigmentation, controlling skin moisture, and protecting against UV rays. However, in spite of this excellent skin improvement effect, vitamin C is very unstable to the external environment and is easily decomposed to lose its physiological activity, and it has a disadvantage of discoloration and swelling. When the concentration is high, stimulation is applied to the skin, It is known to have many problems.

Therefore, the present invention proposes a cosmetic composition which can exhibit more stable and high efficacy by using the Meringa leaves and the upper leaf mixture extracts or the Meringa leaves and the upper leaf mixture extracts using the supercritical extraction method.

The present invention has been conceived to overcome the above-mentioned problems. It is an object of the present invention to provide an antioxidant or antiinflammatory effect of Moringa leaf or upland leaf in natural product extracts. Especially, when supercritical extraction is used as an extraction method, And a cosmetic composition having a superior antioxidant or anti-inflammatory effect because it can extract a large amount of an effective ingredient.

The present invention provides a cosmetic composition comprising a Moringa leaf extract and a leaf extract as an active ingredient.

Wherein the mixture of the Moringa leaves and the upper leaf extract is 0.5 to 20% by weight based on the total weight of the cosmetic composition when the extract is used as a raw material.

The composition may further comprise a natural vegetable oil.

The natural vegetable oil includes glycerin, maltitol and water, and is characterized in that it does not contain any synthetic raw material.

The Moringa leaf extract and the leaf extract are characterized by being extracted with a supercritical extraction method.

The composition according to the present invention can be used as an extract of Moringa leaves, upper leaves or Supercritical Water Extract, so that a larger amount of active ingredient To provide a higher antioxidant or anti-inflammatory effect.

Figure 1 relates to the DPPH free radical scavenging ability of hydrothermal or supercritical extracts from Moringa leaves or dill leaf.
Figure 2 relates to the DPPH free radical scavenging ability of nutritional creams containing Moringa leaves or mulberry leaves.

The cosmetic composition of the present invention contains Moringa leaves and leaf extracts as active ingredients.

The present invention aims to provide a cosmetic composition which has an antioxidative effect and an anti-inflammatory effect by removing active oxygen free radicals, which are the major factors of skin aging, with Moringa leaves and leaf extracts as active ingredients.

In normal cells, some free radicals and other active oxygen and peroxides are produced in the metabolism process. In vivo, however, vitamin E, vitamin E, and antioxidant enzymes such as superoxide dismutase (SOD) C, glutathione, ubiquinone, and uric acid.

As the oxidative stress has proved to be an important cause of various diseases including aging, the possibility of antioxidative bioactive substances having active oxygen scavenging activity as antioxidant inhibitors and disease drugs has been remarkably emphasized. It is required to develop a new antioxidant bioactive substance which can function as a therapeutic agent for diseases.

The Moringa leaves used in the present invention are leaves of Moringa tree. Moringa tree is a plant of Moringaceae family, Moringa genus, Moringa Oleifera (aka Moringa Pterygosperma) One Tamil is also known as Murunggai. Moringa is a soybean plant that leaves and fruits, as well as a whole tree of tropical trees, and contains more than 90 nutrients including amino acids, minerals and vitamins.

In particular, Moringa leaves have cholesterol prevention, anti-inflammatory action, vision improvement, energy increase, blood pressure normalization, skin health recovery, digestive function improvement, immune system strengthening wrinkle / aging prevention, wound treatment improvement, tumor prevention, blood glucose normalization, ulcer prevention And Moringa plants contain over 46 antioxidants and over 36 anti-inflammatory compounds.

The upper leaves used in the present invention are the leaves of the mulberry tree, and there are about 13 kinds of varieties worldwide from the tropics to the temperate regions. The top leaf has been used as a medicinal material in the private sector for over 200 years. The top leaf contains various physiologically active substances such as flavones, steroids and triterpenes, as well as proteins, amino acids, vitamins, minerals and large amounts of dietary fiber.

Especially, it has effective physiological activities such as antidiabetic, antihyperlipidemic, antioxidant and heavy metal removal ability and is effective for antihypertensive, cholesterol lowering and body fat accumulation inhibition, and is effective for prevention of adult diseases.

In the present invention, moringa leaves and upper leaves were extracted by a supercritical extraction method rather than a conventional extraction method of hot water extraction.

Hot water extraction  Process preparation
Supercritical  Extraction technique
Features Item Hot water extraction Supercritical extraction Extraction efficiency lowness height denaturalization Thermal degeneration none Production speed lateness speed Cost / Energy High cost / high energy Low cost / low energy

In the present invention, the supercritical extraction method has been studied in order to secure the stability of a specific active ingredient and to maximize the selective extraction yield in the Moringa leaves and leaf extracts used in the present invention. By extracting in a super critical liquid state having both gas and liquid properties under a specific pressure and a specific temperature condition, it is possible to effectively extract the active ingredient as it is as natural freshness without denaturing by heat and impurities.

By using carbon dioxide as the extraction solvent, it is easy to adjust the critical condition for each ingredient and denaturation of the extract can be minimized by low-temperature process and non-oxidative process. Furthermore, in the present invention, as a cosolvent, butylene glycol, which is a moisturizing material of cosmetics, is combined to impart polarity, thereby maximizing the active ingredient content in the final extract.

Accordingly, an object of the present invention is to provide a cosmetic composition containing extracts of Moringa leaves and upper leaves having antioxidant and antiinflammatory activity in skin, which can delay skin aging and prevent various symptoms of aging due to antioxidative effect and blood circulation promoting action, And to provide a safe and effective cosmetic composition which can be improved.

In order to achieve the above object, the present invention provides an extract obtained by supercritical extraction of Moringa leaves and upper leaves.

Can be obtained by supercritical extraction of the moringa leaves and upper leaves obtained in the present invention at a weight ratio of 0.5 to 2: 0.5 to 1: 0.5 to 1: 0.5 to 2: 0.5 to 1, preferably 1: 1: 1: : 1 in weight ratio. This supercritical extract has a mixed oxidation stability and purity, and is a selective extractable component and a complex extract component containing high active ingredients of antioxidant and antiinflammatory activity.

The composition containing the Moringa leaves and the upper leaves of the present invention is used for the purpose of promoting antioxidation in the skin and is not particularly limited in its formulation. For example, it may be a cosmetic composition having cosmetic formulations of softening agent, nutritional lotion, massage cream, nutritional cream, pack, gel, essence or skin sticking type.

[Example 1] Preparation of Moringa leaves and leaf extracts using hot water extraction method

Dried Meringa leaves and 5 g of the top leaves were added to purified water (100 ml), and the mixture was allowed to stand at 80 for 5 hours to extract the active ingredients. After extraction, the extract was obtained by filtration through a filter having 3 um voids.

[Example 2] Preparation of mixed extracts of Moringa leaves and upper leaves using supercritical extraction

As shown in Fig. 3, 5 g each of the upper leaves was put into a supernatant extraction tank of 100 ml, and the extraction tank was sealed. The temperature of the extraction tank was raised to 40, the high-pressure liquid pump was installed, and the butylene glycol was introduced. At the same time, the carbon dioxide was introduced into the reactor using a high-pressure gas pump to raise the pressure to 300 atm. The pressure and temperature were kept constant at 300 atm and 40 while releasing the extract using a pressure control valve attached to the outlet of the extraction tank. 12.5 g of butylene glycol was added over 30 minutes, and then the operation of the high-pressure liquid pump was stopped. Extraction was completed after extracting only the butylene glycol remaining in the extraction tank for 2 hours with carbon dioxide using a high pressure gas pump. The obtained extract was centrifuged and filtered to be used in the following test.

Five grams of Morangi leaf was placed in a supernatant extraction tank of 100 ml, and the extraction tank was sealed. The temperature of the extraction tank was raised to 40, the high-pressure liquid pump was installed, and the butylene glycol was introduced. At the same time, the carbon dioxide was introduced into the reactor using a high-pressure gas pump to raise the pressure to 300 atm. The pressure and temperature were kept constant at 300 atm and 40 while releasing the extract using a pressure control valve attached to the outlet of the extraction tank. 12.5 g of butylene glycol was added over 30 minutes, and then the operation of the high-pressure liquid pump was stopped. Extraction was completed after extracting only the butylene glycol remaining in the extraction tank for 2 hours with carbon dioxide using a high pressure gas pump. The obtained extract was centrifuged and filtered to be used in the following test.

The extracts obtained by filtration were mixed at a ratio of 1: 1 and used in the test.

[Experimental Example 1] Antioxidant effect of Moringa leaves and leaf extracts and general extracts (Comparison of hot water extraction and supercritical extraction)

<DPPH assay>

1. Preparation

96 well plate

-200ul pipette, 20ul pipette

-pipette tip / foil / E-tube

-DPPH (1,1-dipheyl-2-picrylhydrazyl radical): sigma. Cat. # D-9132

-MeOH & EtOH

-Standard substance: Ascorbic acid

2. Reagent preparation

Preparation of 50 ml of -0.2 mM DPPH (1M: 394.3 g)

: DPPH 0.0039 g (0.2 mM) + MeOH 50 ml

3. Experimental Method

1) Control (Blank): DPPH 150ul + EtOH 50ul

2) Sample: DPPH 150ul + Sample or Standard (100 ~ 500ug / ml) + up to EtOH 50ul

3) 37, 20min (reaction at 10 ~ 30min) Dark reaction (wrapped with foil)

4) Absorbance measurement at 517 nm

-Inhibition (%) = ((Control-test) / control)) * 100

Experimental results of DPPH free radical scavenging ability on the Meringa leaves and leaf extracts using the conventional extraction methods of hot water extraction and supercritical extraction are shown in FIG.

The DPPH free radical scavenging ability of the Meringa leaves and the leaf extracts obtained by the supernatant extraction method using the hot water extraction method was compared with that of the Meringa leaves and leaf extracts obtained using supercritical extraction The antioxidant effect was higher than that of the Moringa leaves and leaf extracts obtained using hot water extraction.

[Preparation Example 1] Preparation of nutritional cream containing Moringa leaves and upper leaf mixed extract

The cosmetic compositions of the formulation examples and comparative formulation examples were prepared according to the composition shown in Table 3 below.

ingredient Content (% by weight) No added extract Extract addition 1. Glycerin 10 10 2. Butylene glycol 10 10 3. Allantoin 0.01 0.01 4 Purified water To100 To100 5. Sodium hyaluronate 0.1 0.1 6. Piggy-60 Hydrogenate Castor Oil 0.5 0.5 7. Glyceryl stearate 0.5 0.5 8. Spices 0.1 0.1 9. Preservatives 2 2 10. Mixed extract of leaf and moringa leaves 0 One

<Formulation Example and Manufacturing Method of Comparative Formulation Example>

1) Ingredients 1 to 5 of Table 1 are mixed and dissolved to prepare a water-based part.

2) Dissolve raw materials 6 to 7 at 65 in a separate beaker to prepare an oil phase.

3) The above-mentioned 2) step oil phase is added to the water phase part (80 or more) and mixed with a homomixer.

4) Add 8 ~ 10 after step 3) and mix.

5) The cosmetic is prepared by removing the air bubbles of the mixture of 4) above.

[Experimental Example 2] Comparison of DPPH free radical scavenging ability of nutritional cream and common nutrient cream containing Moringa leaf and upper leaf mixed extract

The experiment was conducted in the same manner as in [Experimental Example 1].

The results of DPPH free radical scavenging test on nutritional cream and common nutrient cream containing moringa leaves and upper leaf mixed extracts are shown in FIG.

When DPPH free radical scavenging ability of nourishing cream containing moringa leaves and upper leaf mixed extracts and general nutrient cream containing no moringa leaves and upper leaf mixed extracts were compared, The antioxidant effect was higher than that of the normal nutrition cream without the extracts of Moringa leaves and upper leaves.

It is shown that the addition of Moringa leaf and Upper leaf mixed extract can exert higher efficacy.

[Experimental Example 3] Inflammation improvement effect of nutritional cream and common nutrient cream containing moringa leaf and upper leaf blend extract

The inflammatory effect was evaluated by inhibiting the production of prostaglandins. The effect of the extracted extracts on macrophages was measured. First, aspirin was added to the macrophage cells collected from the abdominal cavity of mice to a final concentration of 500 M to irreversibly inhibit the cyclooxygenase (COX) activity remaining in the cells. Then, the electric suspension was incubated in an incubator of 5% CO ₂ and 37 conditions for 2 hours by adding 100 μl to each well of a 96-well cell culture tube to adhere the macrophages to the surface of the container. The adherent macrophages were then washed 3 times with PBS and used for the efficacy of the extract. RPMI medium supplemented with 1% (w / v) LPS was added to 5 x 104 cells / ml of electrophoretic macrophages and cultured for 12 hours to induce the production of prothaglandin. 100 μl of the extract was treated The liberated prostaglandins were quantitated using enzyme immunoassay (ELISA).

At this time, the inhibitory activity of the extract on prostaglandin production was determined by setting the difference of the prostaglandins produced in the LPS-treated group and the untreated group to 100%, and treating the LPS and the sample together to determine the percentage of reduced prostaglandin (Inhibitory effect of prostaglandin production) is shown in Table 2. &lt; tb &gt; &lt; TABLE &gt;

Blank 100% Control group (aspirin treatment group) 25.0% Formulation Example 1 34.5% Comparative Formulation Example 1 14.3%

As shown in the above Table 2, it was found that the inhibitory effect on the production of prostaglandin by Formulation 1 containing moringa leaves and mixed leaf extracts was higher than that of the control group treated with aspirin, It can be confirmed that the effect of inhibiting the production of prostaglandin is much higher than that of Comparative Example 1 in which the mixed extract is not added.

From this, it is shown that the addition of Moringa leaf and Upper leaf mixed extract improves the inflammation improving effect and thus can exert higher efficacy.

Claims (5)

Extract of Moringa leaf and upper leaf extract, which is extracted by supercritical extraction using carbon dioxide and butylene glycol as an extraction solvent,
Cosmetic composition having antioxidant and anti-inflammatory activity. The method according to claim 1,
Characterized in that the moringa leaves and the upper leaves are extracted by supercritical extraction of 0.5 to 20% by weight based on the total weight of the cosmetic composition, and the mixed extract of the Moringa leaf extract and the mulberry leaf extract,
Cosmetic composition having antioxidant and anti-inflammatory activity. delete 3. The method according to claim 1 or 2,
Characterized in that the cosmetic composition comprises glycerin, maltitol and water,
Cosmetic composition having antioxidant and anti-inflammatory activity. delete

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