DE102009045798A1 - Cosmetic agent, useful for lightening e.g. skin, comprises peroxidase and substrate for hydrogen peroxide producing enzyme in a first container, and hydrogen peroxide producing enzyme and substrate for the peroxidase in a second container - Google Patents

Abstract

Cosmetic agent in the form a multi-component packaging unit (kit-of-parts), comprises: a cosmetic composition (A) in a first container containing at least one peroxidase of the enzyme class with the enzyme commission number (1.11.1.x) for skin lightening, where x is 1-3 to 5-16, and at least a substrate for a hydrogen peroxide producing enzyme, which is present in a second container; and a cosmetic composition (B) in the second container containing at least one hydrogen peroxide producing enzyme for skin lightening and optionally at least a substrate for the peroxidase in the first container. An independent claim is also included for a non-therapeutic, cosmetic method for lightening the skin and/or age spots or freckles, comprising: mixing the composition (A) and (b); and applying the resulting mixture on the skin. ACTIVITY : Dermatological. No biological data given. MECHANISM OF ACTION : None given.

Description

The The present invention relates to cosmetic or dermatological Compositions for cosmetic and topical dermatological Skin lightening or to prevent skin tanning, in particular the skin tanning caused by UV radiation, and to lighten age spots or freckles.

The Pigmentation of the skin takes place through melanocytes, which in the lowest layer of the epidermis next to the basal cells as depending on Skin type either sporadic or frequently occurring pigment-forming Cells are found. Melanocytes contain melanosomes in which Melanin is formed. Through various chemical and / or physical Influences, in particular by UV radiation, is amplified Melanin formed. This is about the keratinocytes in the corneocytes (horny layer) transported and leads to a brownish to brown-black skin color. melanin is formed as the final stage of an oxidative process, in which Tyrosine with the participation of enzyme tyrosinase over several Intermediate stages to the brown to brown-black Eumelaninen (DHICA- and DHI-melanin) or involving sulfur-containing compounds is converted to reddish phaeomelanin. The Melanin formation - and thus skin color - is subject to external Influences and can beside desired effects ("Healthy tan") too unwanted Apparitions lead. So z. B. UV radiation to freckles to lead. Fehlpigmentierungen can also due to genetic disposition, wound healing or scarring or skin aging ("Age spots") occur. Since the process of Desquamation (superficial detachment of cells or cell groups from their epithelial association) has a consistent Loss of melanin may result in skin lightening due to inhibition the new synthesis of melanin can be achieved.

age spots represent a sign of skin aging, that of a special treatment requirement. Since it is not a more intense pigmentation of the skin, but concretely about accumulations of the brownish-waxy Pigments Lipofuscin (also age or wear pigment), which is known as End product from the oxidation of unsaturated fatty acids the cell membranes arises. The lysosomes are no longer able to completely degrade the substance. So he stays as a stain back. Effective cosmetic compositions for skin lightening are known. These contain as active ingredient but mostly hydroquinone or kojic acid (for inhibiting tyrosinase). Both Substances are mutagenic and therefore should not last longer Periods are used.

It was therefore still the task of skin lightening compositions to make available, free from the disadvantages mentioned are.

Surprisingly, it has been found that age spots and skin color can be treated and lightened by enzymatic bleaching. In this case so-called peroxidases of the enzyme class (EC) 1.11.1.x with x = 1-3 and 5-16 are used, which in the presence of hydrogen peroxide lead to a bleaching of the age spots and the natural skin color. The hydrogen peroxide is provided in situ by H ₂ O ₂ producing enzymes, such as oxidases or superoxide dismutases. In order for this reaction to occur only when the product is applied to the skin, it is necessary to work with dual-chamber systems. The chambers then each contain a formulation containing at least one peroxidase of the enzyme class (EC) 1.11.1.x with x = 1-3 and 5-16 and the substrate for the complementary, H ₂ O ₂ -producing enzyme, which together with a substrate for the peroxidase in the second chamber.

The oxidative treatment of the skin by H ₂ O ₂ or peroxides, such as benzoyl peroxide, is already known in the art. Hydrogen peroxide causes the breakdown of the natural skin pigment melanin. However, the hydrogen peroxide effect simultaneously leads to an undesired, unselective damage to the skin structure or to a relatively rapid oxidation, which can bring about a spotty, uneven bleaching result. The greater the amount of hydrogen peroxide used, the more pronounced are the disadvantages described above. Finally, high concentrations of oxidizing agents can cause skin irritation to sensitive users and are therefore not considered optimal.

Surprisingly, it has now been found that the abovementioned disadvantages can be avoided if the H ₂ O ₂ is provided in situ by enzymes, such as oxidases or superoxide dismutases.

WO 2004017931 A1 discloses enzymatic skin lightening agents which contain an effective content of enzymes to which the copper in the prosthetic group has been removed by a prior exchange reaction, or to such modified copper binding proteins produced by recombinant methods were made without copper. Such modified copper proteins are loud WO 2004017931 A1 capable of effectively removing the copper from the enzyme tyrosinase, which is also a copper protein, thereby disrupting the natural melanin synthesis that occurs via the non-essential amino acid tyrosine. An oxidizing effect of these enzymes is not disclosed.

suitable Oxidases, superoxide dismutases and peroxidases are from plants, Mushrooms, bacteria or algae available. They either come from originally from microorganisms, such as the genera Bacillus, Streptomyces, Humicola, or Pseudomonas, and / or are known per se biotechnological processes produced by suitable microorganisms, for example by transgenic expression hosts of the genera Bacillus or through filamentous mushrooms. The purification of the concerned Enzymes takes place in a favorable manner via established ones Process, for example by precipitation, sedimentation, Concentration, filtration of the liquid phases, microfiltration, Ultrafiltration, exposure to chemicals, deodorization or appropriate combinations of these steps. Furthermore, the enzymes can be used together with Accompanying substances, such as from fermentation, or with stabilizers be made up.

• – in einem ersten Container eine kosmetische Zusammensetzung (A), enthaltend eine zur Hautaufhellung wirksame Menge mindestens einer Peroxidase der Enzymklasse mit der Enzyme Commission Number (EC) 1.11.1.x mit x = 1–3 und 5–16 sowie mindestens ein zur Hautpflege geeignetes Substrat für ein H₂O₂ produzierendes Enzym, das in dem zweiten Container enthalten ist,
• – in einem zweiten Container eine kosmetische Zusammensetzung (B), enthaltend eine zur Hautaufhellung wirksame Menge mindestens eines H₂O₂ produzierenden Enzyms sowie – sofern erforderlich – mindestens ein zur Hautpflege geeignetes Substrat für die Peroxidase der Enzymklasse mit der Enzyme Commission Number (EC) 1.11.1.x mit x = 1–3 und 5–16, die in dem ersten Container enthalten ist,

wobei das Mittel frei ist von Substanzen, die nicht zur Hautpflege geeignet sind.The present invention is a cosmetic agent for lightening the skin and / or age spots, which is in the form of a multi-component packaging unit (kit-of-parts), comprising

• In a first container, a cosmetic composition (A) comprising an effective skin lightening amount of at least one peroxidase enzyme class with the Enzyme Commission Number (EC) 1.11.1.x with x = 1-3 and 5-16 and at least one of Skin care suitable substrate for an H ₂ O ₂ producing enzyme contained in the second container,
• In a second container a cosmetic composition (B) containing an effective skin lightening amount of at least one H ₂ O ₂ -producing enzyme and - if necessary - at least one suitable for skin care substrate for the peroxidase of the enzyme class with the Enzyme Commission Number (EC) 1.11.1.x with x = 1-3 and 5-16, which is contained in the first container,

wherein the agent is free of substances that are not suitable for skin care.

Due to the enzymatic in situ production of H ₂ O ₂ , there are no local excess concentrations that could lead to irritation or even damage to the skin compared to conventional oxidative skin lightening agents which contain H ₂ O ₂ or an organic peroxide in the first place.

Under a substance suitable for skincare is inventively a Substance understood in accordance with the European Cosmetic Directive 76/768 / EEC authorized for skin care products is. Oxidative hair dyes and their dye precursors as well directly pulling hair dyes are according to the European Cosmetics Directive 76/768 / EEC for Skin care products are not approved and provide accordingly no suitable for skin care substances.

The preferred peroxidase enzyme activity in use is 1 to 1000 peroxidase units (U) per 100 g of finished Skin whitening agent according to the invention (= composition (A) plus composition (B)). Particularly preferred are agents with a peroxidase activity of 3 to 500 U / 100 g, in particular 10 to 150 U / 100 g and especially 15 to 100 U / 100 g finished inventive Skin lightening agent. Calculated on pure peroxidase enzyme the activity at more than 100 U / mg (25 ° C).

The Determination of peroxidase activity has been known for a long time and described in various publications.

The at least one peroxidase of the enzyme class (EC) 1.11.1.x with x = 1-3 and 5-16 is preferred in an amount of 0.0001 to 10 wt .-%, preferably 0.001 to 3 wt .-%, particularly preferably 0.01-1 wt%, most preferably 0.1-0.5 Wt .-%, each based on the protein amount of the enzyme in the whole cosmetic composition (A).

The preferred enzyme activity of the H ₂ O ₂ -producing enzyme in use is 1 to 1000 oxidase units or superoxide dismutase units (U) per 100 g of finished skin lightening agent according to the invention. Particularly preferred are agents having an oxidase activity or superoxide dismutase activity of 3 to 500 U / 100 g, in particular 10 to 150 U / 100 g and especially 15 to 100 U / 100 g of finished skin-lightening agent according to the invention. Calculated on pure H ₂ O ₂ producing enzyme, the activity is more than 100 U / mg (25 ° C).

The determination of oxidase activity or superoxide dismutase activity has long been known and described in various publications.

The at least one H ₂ O ₂ -producing enzyme is preferably in an amount of 0.0001 to 10 wt .-%, preferably 0.001 to 3 wt .-%, particularly preferably 0.01 to 1 wt .-%, most preferably 0, 1-0.5 wt .-%, each based on the protein amount of the enzyme in the entire cosmetic composition (B).

The skin lightening agents according to the invention contain the H ₂ O ₂ -producing enzymes and the peroxidase or catalase preferably in an enzyme activity ratio (units) of 1:10 to 10: 1, more preferably 1: 3 to 3: 1, extremely 1: 2 to 2: 1, most preferably 1: 1.

Compositions ^according to the invention preferably contain enzymes in an amount of 1 × 10 ^-8 to 5% by weight, based on active protein, in the entire cosmetic composition (A) or (B). Preferably, the enzymes are from 0.001 to 5% by weight, more preferably from 0.01 to 5% by weight, even more preferably from 0.05 to 4% by weight and most preferably from 0.075 to 3.5% by weight. % contained in a composition according to the invention.

The protein concentration in the composition can be determined by known methods, for example the BCA method (bicinchoninic acid, 2,2'-biquinolyl-4,4'-dicarboxylic acid) or the biuret method ( Gornall AG, CS Bardawill and MM David, J. Biol. Chem., 177 (1948), pp. 751-766 ).

Skin-lightening agents preferred according to the invention are characterized by a cosmetic composition (B) which contains in a container an effective amount of at least one H ₂ O ₂ -producing enzyme selected from:
1.1.3.3 Malate Oxidase
1.1.3.4 glucose oxidase
1.1.3.5 hexose oxidase
1.1.3.6 cholesterol oxidase
1.1.3.7 Aryl Alcohol Oxidase, Veratryl Alcohol Oxidase
1.1.3.8 L-gulonolactone oxidase
1.1.3.9 galactose oxidase
1.1.3.10 Pyranose oxidase
1.1.3.11 L-sorbose oxidase
1.1.3.12 Pyridoxine 4-oxidase
1.1.3.13 Alcohol Oxidase, Ethanol Oxidase
1.1.3.15 Glycolate Oxidase, (S) -2-Hydroxy Acid Oxidase
1.1.3.16 ecdysone oxidase
1.1.3.17 choline oxidase
1.1.3.18 oxidase for secondary alcohols
1.1.3.19 4-hydroxymandelate oxidase (decarboxylating)
1.1.3.21 Glycerol-3-phosphate oxidase
1.1.3.23 thiamine oxidase
1.1.3.27 hydroxyphytanate oxidase
1.1.3.29 N-acylhexosamine oxidase
1.1.3.30 polyvinyl alcohol oxidase; PVA oxidase
1.1.3.37 D-arabinono-1,4-lactone oxidase
1.1.3.38 Vanillyl alcohol oxidase
1.1.3.39 Nucleoside oxidase (H ₂ O ₂ -forming)
1.1.3.40 D-mannitol oxidase
1.1.3.41 Alditol oxidase (xylitol oxidase)
1.2.3.1 aldehyde oxidase, quinoline oxidase
1.2.3.3 Pyruvate oxidase
1.2.3.4 Oxalate oxidase
1.2.3.5 Glyoxylate oxidase
1.2.3.6 Pyruvate oxidase
1.2.3.7 indole-3-acetaldehyde oxidase
1.2.3.9 Aryl aldehyde oxidase
1.2.3.11 Retinal Oxidase
1.2.3.14 Abscisinaldehyde oxidase
1.3.3.1 Dihydroorotate oxidase
1.3.3.4 Protoporphyrinogen oxidase
1.3.3.6 Acyl-CoA oxidase
1.3.3.7 Dihydrouracil oxidase
1.3.3.8 Tetrahydroberberine oxidase
1.3.3.10 tryptophan α, β-oxidase
1.3.3.11 Pyrroloquinoline Quinone Synthase, PqqC
1.3.3.12 L-galactonolactone oxidase
1.4.3.1 D-aspartate oxidase
1.4.3.2 L-amino acid oxidase
1.4.3.3 D-amino acid oxidase
1.4.3.4 monoamine oxidase
1.4.3.5 Pyridoxal 5'-phosphate synthase
1.4.3.7 D-glutamate oxidase
1.4.3.8 ethanolamine oxidase
1.4.3.10 Putrescine oxidase
1.4.3.11 L-glutamate oxidase
1.4.3.12 cyclohexylamine oxidase
1.4.3.13 Protein lysine 6-oxidase, lysine oxidase
1.4.3.14 L-lysine oxidase
1.4.3.15 D-glutamate oxidase, D-aspartate oxidase
1.4.3.16 L-aspartate oxidase
1.4.3.19 glycine oxidase
1.4.3.20 L-lysine 6-oxidase
1.4.3.21 Primary amine oxidase
1.4.3.22 Diamine oxidase
1.5.3.1 Sarcosine oxidase
1.5.3.2 N-methyl-L-amino acid oxidase
1.5.3.4 N ⁶ -methyllysine oxidase
1.5.3.5 (S) -6-hydroxynicotin oxidase
1.5.3.6 (R) -6-hydroxynicotin oxidase
1.5.3.7 L-pipecolate oxidase
1.5.3.10 dimethylglycine oxidase
1.5.3.11 Polyamine oxidase
1.5.3.12 Dihydrobenzophenanthridine oxidase
1.6.3.1 NAD (P) H Oxidase
1.7.3.1 Nitroalkane oxidase
1.7.3.3 Uricase (urate oxidase)
1.7.3.5 3-Acid-nitropropanoate oxidase
1.8.3.1 Sulfite oxidase
1.8.3.2 Thiol oxidase, sulfhydryl oxidase
1.8.3.3 Glutathione oxidase
1.8.3.4 methanethiol oxidase
1.8.3.5 Prenylcysteine oxidase
1.10.3.5 3-hydroxyanthranilate oxidase
1.10.3.6 Rifamycin B oxidase
1.15.1.1 Superoxide dismutase
1.15.1.2 Superoxide Reductase
1.15.1.3 Superoxide dismutase
1.17.3.2 Xanthine oxidase
1.17.3.3 6-hydroxynicotinate dehydrogenase
1.21.3.3 reticulin oxidase.

Particularly preferred H ₂ O ₂ -producing enzymes are selected from the abovementioned enzymes of EC 1.1.3.x, which have an alpha-hydroxycarboxylic acid or its salt as substrate, in particular EC 1.1.3.3 (malate oxidase, substrate: (S) - (-) - hydroxysuccinic acid and its salts), EC 1.1.3.8 (L-gulonolactone oxidase, substrate: L-gulono-1,4-lactone, L-gulono-γ-lactone), EC 1.1.3.15 (glycolate oxidase , (S) -2-hydroxy acid oxidase, in A-form and B-form, wherein the A-form is preferred; A-form substrates: short-chain aliphatic (S) -alpha-hydroxycarboxylic acids, especially (S) -glycolic acid , (S) -lactic acid, tartaric acid, citric acid, (S) -2-hydroxybutanoic acid, 2,3-dihydroxypropanoic acid, 2-hydroxypentanoic acid, 2-hydroxyhexanoic acid, 2-hydroxymalonic acid (tartronic acid), (S) -malic acid, 2-hydroxy- 2-methylsuccinic acid, Erythrarsäu re, threaric, glucaric, galactaric, mannaric, gularic, gluconic, glucuronic and galacturonic acids; Substrates of the B form: long-chain and aromatic (S) -alpha-hydroxycarboxylic acids, in particular 2-hydroxyheptanoic acid, 2-hydroxyoctanoic acid, 2-hydroxynonanoic acid, 2-hydroxydecanoic acid, 2-hydroxydodecanoic acid, 2-hydroxytetradecanoic acid, 2-hydroxyhexadecanoic acid, 2-hydroxyoctadecanoic acid, mandelic acid and 4-hydroxymandelic acid), further EC 1.1.3.19 (4-hydroxymandelate oxidase (decarboxylating), substrate: 4-hydroxymandelic acid and 4-hydroxymandelate, respectively).

Further particularly preferred H ₂ O ₂ -producing enzymes are selected from the abovementioned enzymes of EC 1.1.3.x, which have a mono- or disaccharide or an N-acylamine of a mono- or disaccharide as substrate, in particular EC 1.1.3.4 (glucose -Oxidase, substrate: β-D-glucose), EC 1.1.3.5 (hexose oxidase, substrates: β-D-glucose, D-galactose, D-mannose, maltose, lactose and cellobiose), EC 1.1.3.9 (galactose -Oxidase, substrate: β-D-galactose), EC 1.1.3.10 (pyranose oxidase, preferred substrates: D-glucose, D-xylose, L-sorbose and D-glucono-1,5-lactone), EC 1.1. 3.11 (L-sorbose oxidase, substrates: L-sorbose, D-glucose, D-galactose and D-xylose), EC 1.1.3.29 (N-acylhexosamine oxidase, preferred substrates: N-acetyl-D-glucosamine and N Acetyl-D-galactosamine), further EC 1.1.3.37 (D-arabinono-1,4-lactone oxidase, preferred substrates: D-arabinono-1,4-lactone, L-galactono-1,4-lactone, L -Xylono-1,4-lactone), further EC 1.1.3.40 (D-Mannitol Oxidase Preferred Substrates: D-Mannitol and D- Arabinitol) and EC 1.1.3.41 (alditol oxidase, xylitol oxidase, preferred substrates: xylitol and sorbitol, furthermore L-threitol, D-arabitol, D-galactitol and D-mannitol).

Further particularly preferred H ₂ O ₂ -producing enzymes are selected from the abovementioned enzymes of EC 1.1.3.x, which have an alcohol as substrate, in particular EC 1.1.3.7 (aryl alcohol oxidase, veratryl alcohol oxidase, substrates: primary alcohols having an aromatic ring, in particular (2-naphthyl) methanol and 3-methoxybenzyl alcohol), furthermore EC 1.1.3.13 (alcohol oxidase, ethanol oxidase, substrates: short-chain primary alcohols, which may be unsaturated, preferably ethanol, 1-propanol, 1-butanol and 1- Pentanol), furthermore EC 1.1.3.18 (oxidase for secondary alcohols, preferred substrates: secondary alcohols having five or more carbon atoms, in particular 1-menthol, 2-pentanol, 3-pentanol, 2-hexanol, 3-hexanol, 2-heptanol, 3-heptanol, 2-octanol, 1,3-cyclohexanediol, 2,4-pentanediol, 2,5-hexanediol, but also 1,2-propanediol, 2-butanol or 1,3-butanediol, and polyvinyl alcohols having molecular weights of 300 Dalton and above), EC 1.1.3.30 (polyvinyl alcohol oxidase PVA oxidase, preferred substrates: polyvinyl alcohol) and EC 1.1.3.38 (vanillyl alcohol oxidase, preferred substrates: various 4-hydroxybenzyl alcohols and 4-hydroxybenzylamines, in particular vanillyl alcohol (4-hydroxy-3-methoxybenzyl alcohol) and 4-hydroxybenzyl alcohol).

Another particularly preferred H ₂ O ₂ -producing enzyme of EC 1.1.3.x is EC 1.1.3.6 (cholesterol oxidase, preferred substrates: cholesterol, beta-sitosterol, beta-stigmasterol, DHEA (dehydroepiandrosterone).

Another particularly preferred H ₂ O ₂ -producing enzyme of EC 1.1.3.x is EC 1.1.3.12 (pyridoxine-4-oxidase, preferred substrates: pyridoxine, pyridoxamine, pyridoxine-5'-phosphate or pyridoxamine-5'-phosphate, the biologically active forms of vitamin 66).

Another particularly preferred H ₂ O ₂ producing enzyme of EC 1.1.3.x is EC 1.1.3.17 (choline oxidase, preferred substrates: choline and betaine aldehyde).

Another particularly preferred H ₂ O ₂ -producing enzyme of EC 1.1.3.x is EC 1.1.3.39 (nucleoside oxidase (H ₂ O ₂ -forming), preferred substrate: adenosine).

A particularly preferred H ₂ O ₂ -producing enzyme of EC 1.2.3.x is EC 1.2.3.9 (aryl aldehyde oxidase, preferred substrates: benzaldehyde, vanillin and various other aromatic aldehydes).

A particularly preferred H ₂ O ₂ -producing enzyme of EC 1.3.3.x is EC 1.3.3.12 (L-galactonolactone oxidase, preferred substrates: the 1,4-lactones of L-galactonic acid, D-altronic acid, L-fuconic acid, D-arabinonic acid and D-threonic acid).

Further particularly preferred H ₂ O ₂ -producing enzymes are selected from the abovementioned enzymes of EC 1.4.3.x and 1.5.3.x, which have an amino acid or its salt as substrate, in particular EC 1.4.3.2 (L-amino acid oxidase preferred substrates: L-methionine, L-tryptophan, L-leucine, L-histidine, L-phenylalanine, L-arginine, L-isoleucine, L-alanine, L-asparagine, L-aspartic acid, L-tyrosine, L- Valine, L-citrulline, L-cysteine, L-cystine, L-glutamine, L-glutamic acid, L-hydroxylysine, L-hydroxyproline, L-lysine, L-ornithine, L-Pro lin, L-homophenylalanine, L-norleucine, LO-methylserine, L-2,3-diaminopropionic acid, L-2,4-diaminobutyric acid, L-2-amino-4,4-dimethylvaleric acid, L-2-amino-4, 4-dimethylhexanoic acid, L-homoserine lactone, glycine), furthermore EC 1.4.3.11 (L-glutamate oxidase, preferred substrates: only L-glutamic acid and L-glutamate), furthermore EC 1.4.3.13 (protein lysine 6-oxidase, Lysine oxidase, preferred substrates: peptidyl-L-lysyl peptides and polylysine), further EC 1.4.3.14 (L-lysine oxidase, preferred substrates: L-lysine and other alpha, omega-diaminomonocarboxylates and L-lysine hydroxamates) EC 1.4.3.16 (L-aspartate oxidase, preferred substrate: L-aspartic acid and L-aspartates), further EC 1.4.3.19 (glycine oxidase, preferred substrates of glycine oxidase from Bacillus subtilis: glycine, D-alanine, sarcosine , N-ethylglycine, D-α-aminobutyrate, D-proline, D-pipecolic acid (piperidine-2-carboxylic acid), N-methyl-D-alanine), further EC 1.4.3.20 (L-lysine 6-oxidase, preferred Substrates: L-lysine, 2-NA cetyl-L-lysine, L-ornithine, D-lysine and 4-hydroxy-L-lysine) and EC 1.5.3.1 (sarcosine oxidase, preferred substrate: sarcosine).

Although the aforementioned H ₂ O ₂ -producing enzymes of EC 1.4.3.x also form ammonia, but this is immediately intercepted by the excess of excess amino acid substrate (with the exception of cysteine) and bound as ammonium ion).

Further particularly preferred H ₂ O ₂ -producing enzymes are selected from the abovementioned enzymes of EC 1.4.3.x, which have an amine as a substrate, in particular EC 1.4.3.8 (ethanolamine oxidase, preferred substrates: ethanolamine and 3-amino-1 -propanol) and EC 1.4.3.10 (putrescine oxidase, preferred substrates: putrescine = butane-1,4-diamine = tetramethylenediamine, for putrescine oxidase from Candida guilliermondii also spermidine = N- (3-aminopropyl) -1,4- butanediamine).

Another particularly preferred H ₂ O ₂ -producing enzyme of EC 1.4.3.x is EC 1.4.3.5 (pyridoxal-5'-phosphate synthase, preferred substrates: pyridoxine, pyridoxamine, pyridoxine-5'-phosphate or pyridoxamine-5 ' phosphate, the biologically active forms of vitamin 66).

Another particularly preferred enzyme producing H ₂ O ₂ is EC 1.7.3.3 (uricase, urate oxidase), whose preferred substrates, uric acid and its salts (= urates), are degraded to allantoin, a skin-care active substance.

Another particularly preferred H ₂ O ₂ -producing enzyme is EC 1.17.3.2 (xanthine oxidase, preferred substrates: caffeine, theophylline, theobromine).

According to the invention, particularly preferred skin care substrates for the H ₂ O ₂ -producing enzymes are selected from alpha-hydroxycarboxylic acids having 2 to 24 carbon atoms, their salts and their lactones, in particular (S) - (-) - malic acid and its salts, L-Gulono -1,4-lactone (L-gulono-γ-lactone), D-glucono-1,5-lactone, D-arabinono-1,4-lactone, L-galactono-1,4-lactone, L-xylono- 1,4-lacsone, the 1,4-lactones of L-galactonic acid, D-altronic acid, L-fuconic acid, D-arabinonic acid and D-threonic acid, short-chain aliphatic (S) -alpha-hydroxycarboxylic acids having 2 to 6 carbon atoms and their Salts in particular (S) -glycolic acid, (S) -lactic acid, tartaric acid, citric acid, (S) -2-hydroxybutanoic acid, 2,3-dihydroxypropanoic acid, 2-hydroxypentanoic acid, 2-hydroxyhexanoic acid, 2-hydroxymalonic acid (tartronic acid), (S) -Malic acid, 2-hydroxy-2-methylsuccinic acid, erythraric acid, threaric acid, glucaric acid, galactaric acid, mannaric acid, gular acid, gluconic acid e, glucuronic acid and galacturonic acid; long-chain and aromatic (S) -alpha-hydroxycarboxylic acids having 7 to 24 carbon atoms and their salts, in particular 2-hydroxyheptanoic acid, 2-hydroxyoctanoic acid, 2-hydroxynonanoic acid, 2-hydroxydecanoic acid, 2-hydroxydodecanoic acid, 2-hydroxytetradecanoic acid, 2-hydroxyhexadecanoic acid, 2-hydroxyoctadecanoic acid Mandelic acid, 4-hydroxymandelic acid and 4-hydroxymandelate; Mono- or disaccharides, in particular D-glucose, β-D-glucose, D-galactose. β-D-galactose, D-mannose, D-mannitol, D-arabinitol, D-xylose, L-sorbose, maltose, lactose, cellobiose, xylitol, sorbitol, L-threitol, D-arabitol and D-galactitol; N-acylamines of a mono- or disaccharide, especially N-acetyl-D-glucosamine and N-acetyl-D-galactosamine; primary alcohols having an aromatic ring, especially (2-naphthyl) methanol and 3-methoxybenzyl alcohol; short-chain primary alcohols having 2 to 8 carbon atoms, which may be unsaturated, preferably ethanol, 1-propanol, 1-butanol and 1-pentanol; secondary alcohols having five to 12 carbon atoms, in particular 1-menthol, 2-pentanol, 3-pentanol, 2-hexanol, 3-hexanol, 2-heptanol, 3-heptanol, 2-octanol, 1,3-cyclohexanediol, 2,4 Pentanediol, 2,5-hexanediol, but also 1,2-propanediol, 2-butanol or 1,3-butanediol; Polyvinyl alcohols having molecular weights of 300 daltons and above; 4-hydroxybenzyl alcohols and 4-hydroxybenzylamines, especially vanillyl alcohol (4-hydroxy-3-methoxybenzyl alcohol) and 4-hydroxybenzyl alcohol; Cholesterol, beta-sitosterol, beta-stigmasterol, DHEA (dehydroepiandrosterone); Pyridoxine, pyridoxamine, pyridoxine 5'-phosphate and pyridoxamine 5'-phosphate; Choline, betaine aldehyde; Nucleosides, especially adenosine; Benzaldehydes, in particular vanillin; Amino acids and their salts, in particular L-methionine, L-tryptophan, L-leucine, L-Hi stidine, L-phenylalanine, L-arginine, L-isoleucine, L-alanine, L-asparagine, L-aspartic acid, L-aspartates, L-tyrosine, L-valine, L-citrulline, L-cysteine, L-cystine, L-glutamine, L-glutamic acid, L-glutamates, L-hydroxylysine, L-hydroxyproline, L-lysine, 1-ornithine, L-proline, L-homophenylalanine, L-norleucine, L-methylserine, L-2,3- Diaminopropionic acid, L-2,4-diaminobutyric acid, L-2-amino-4,4-dimethylvaleric acid, L-2-amino-4,4-dimethylhexanoic acid, L-homoserine lactone, glycine, D-alanine, sarcosine, N-ethylglycine, D-α-aminobutyrate, D-proline, D-pipecolic acid (piperidine-2-carboxylic acid), N-methyl-D-alanine, 2-N-acetyl-L-lysine, D-lysine and 4-hydroxy-L-lysine; Peptidyl-L-lysyl peptides and polylysine; alpha, omega-diaminomonocarboxylates and L-lysine hydroxamates; Ethanolamine, 3-amino-1-propanol, putrescine = butane-1,4-diamine = tetramethylenediamine, spermidine = N- (3-aminopropyl) -1,4-butanediamine); Uric acid and its salts (= urates), caffeine, theophylline and theobromine.

Skin whitening agents which are preferred according to the invention are characterized by a cosmetic composition (A) which comprises at least one skin care substrate for the H ₂ O ₂ -producing enzyme contained in the composition (B) in a total amount of from 0.1 to 10% by weight 0.5 to 7 wt .-%, particularly preferably 1 to 3 wt .-%, based on the total weight of the composition (A).

Skin whitening agents which are preferred according to the invention are characterized by a cosmetic composition (A) which contains an effective amount of at least one peroxidase of the enzyme class EC 1.11.1.x with x = 1-3 and 5-16, which is selected from:
EC 1.11.1.1 NADH peroxidase,
EC 1.11.1.2 NADPH peroxidase,
EC 1.11.1.3 fatty acid peroxidase,
EC 1.11.1.5 cytochrome c peroxidase,
EC 1.11.1.6 Catalase,
EC 1.11.1.7 Peroxidase (synonym: lactoperoxidase, horseradish peroxidase),
EC 1.11.1.8 iodide peroxidase,
EC 1.11.1.9 glutathione peroxidase,
EC 1.11.1.10 Chloride peroxidase,
EC 1.11.1.11 L-ascorbate peroxidase,
EC 1.11.1.12 phospholipid hydroperoxide glutathione peroxidase,
EC 1.11.1.13 manganese peroxidase,
EC 1.11.1.14 lignin peroxidase,
EC 1.11.1.15 Peroxiredoxin and
EC 1.11.1.16 Hybrid peroxidase (versatile peroxidase).

Particularly according to the invention preferred are peroxidases of EC 1.11.1.3 (fatty acid peroxidase, preferred substrates are fatty acids having 12 to 18 carbon atoms, especially n-dodecanoic acid (lauric acid), n-tridecanoic acid, n-tetradecanoic acid, n-pentadecanoic acid, n-hexadecanoic acid (Palmitic acid), n-heptadecanoic acid and n-octadecanoic acid (Stearic acid), with palmitic acid being especially preferred), EC 1.11.1.6 (catalase), EC 1.11.1.7 (peroxidase, especially horseradish peroxidase), EC 1.11.1.11 (L-ascorbate oxidase, with L-ascorbic acid and its salts as preferred substrates) and EC 1.11.1.14 (lignin peroxidase, preferred substrate: 3,4-dimethoxybenzyl alcohol).

Particularly according to the invention preferred skin care substrates for the enzymes the enzyme class EC 1.11.1.x with x = 1-3 and 5-16 are selected from fatty acids having 12 to 18 carbon atoms, above all n-dodecanoic acid (lauric acid), n-tridecanoic acid, n-tetradecanoic acid, n-pentadecanoic acid, n-hexadecanoic acid (Palmitic acid), n-heptadecanoic acid and n-octadecanoic acid (Stearic acid), with palmitic acid being especially preferred is L-ascorbic acid and its salts, and 3,4-dimethoxybenzyl alcohol.

Further According to the invention, preferred skin lightening agents are characterized by a cosmetic composition (B), the at least one skin care suitable substrate for the peroxidase of the enzyme class contained in the composition (A) EC 1.11.1.x with x = 1-3 and 5-16 in a total of 0.1 to 10 wt .-%, preferably 0.5 to 7 wt .-%, particularly preferably 1 to 3 wt .-%, based on the total weight of the composition (B), contains.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing glucose oxidase, and a cosmetic composition (A) containing catalase.

Further skin-whitening agents which are particularly preferred according to the invention comprise a cosmetic Composition (B) containing glucose oxidase, and a cosmetic composition (A) containing peroxidase EC 1.11.1.7.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing glucose oxidase, and a cosmetic composition (A) containing L-ascorbate peroxidase.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing hexose oxidase, and a cosmetic composition (A) containing catalase.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing hexose oxidase, and a cosmetic composition (A) containing peroxidase EC 1.11.1.7.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing hexose oxidase, and a cosmetic composition (A) containing L-ascorbate peroxidase.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing cholesterol oxidase, and a cosmetic composition (A) containing fatty acid peroxidase.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing cholesterol oxidase, and a cosmetic composition (A) containing catalase.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing cholesterol oxidase, and a cosmetic composition (A) containing peroxidase EC 1.11.1.7.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing cholesterol oxidase, and a cosmetic composition (A) containing L-ascorbate peroxidase.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing aryl alcohol oxidase EC 1.1.3.7, and a cosmetic composition (A) containing Fettsäureperoxidase.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing aryl alcohol oxidase EC 1.1.3.7, and a cosmetic composition (A) containing Catalase.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing aryl alcohol oxidase EC 1.1.3.7, and a cosmetic composition (A) containing Peroxidase EC 1.11.1.7.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing aryl alcohol oxidase EC 1.1.3.7, and a cosmetic composition (A) containing L-ascorbate peroxidase.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing galactose oxidase, and a cosmetic composition (A) containing fatty acid peroxidase.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing galactose oxidase, and a cosmetic composition (A) containing catalase.

Further skin-whitening agents which are particularly preferred according to the invention comprise a cosmetic Composition (B) containing galactose oxidase, and a cosmetic composition (A) containing peroxidase EC 1.11.1.7.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing galactose oxidase, and a cosmetic composition (A) containing L-ascorbate peroxidase.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing pyranose oxidase, and a cosmetic composition (A) containing fatty acid peroxidase.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing pyranose oxidase, and a cosmetic composition (A) containing catalase.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing pyranose oxidase, and a cosmetic composition (A) containing peroxidase EC 1.11.1.7.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing pyranose oxidase, and a cosmetic composition (A) containing L-ascorbate peroxidase.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing pyranose oxidase, and a cosmetic composition (A) containing lignin peroxidase.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing L-sorbose oxidase, and a cosmetic composition (A) containing catalase.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing L-sorbose oxidase, and a cosmetic composition (A) containing peroxidase EC 1.11.1.7.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing L-sorbose oxidase, and a cosmetic composition (A) containing L-ascorbate peroxidase.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing pyridoxine 4-oxidase, and a cosmetic composition (A) containing fatty acid peroxidase.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing pyridoxine 4-oxidase, and a cosmetic composition (A) containing catalase.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing pyridoxine 4-oxidase, and a cosmetic composition (A) containing peroxidase EC 1.11.1.7.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing pyridoxine 4-oxidase, and a cosmetic composition (A) containing L-ascorbate peroxidase.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing alcohol oxidase EC 1.1.3.13, and a cosmetic composition (A) containing Fettsäureperoxidase.

Further skin-whitening agents which are particularly preferred according to the invention comprise a cosmetic Composition (B) containing alcohol oxidase EC 1.1.3.13, and a cosmetic composition (A) containing catalase.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing alcohol oxidase EC 1.1.3.13, and a cosmetic composition (A) containing Peroxidase EC 1.11.1.7.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing alcohol oxidase EC 1.1.3.13, and a cosmetic composition (A) containing L-ascorbate peroxidase.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing the A-form the (S) -2-hydroxy acid oxidase, and a cosmetic composition (A) containing fatty acid peroxidase.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing the A-form the (S) -2-hydroxy acid oxidase, and a cosmetic composition (A) containing catalase.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing the A-form the (S) -2-hydroxy acid oxidase, and a cosmetic composition (A) containing peroxidase EC 1.11.1.7.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing the A-form the (S) -2-hydroxy acid oxidase, and a cosmetic composition (A) containing L-ascorbate peroxidase.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing the B-form the (S) -2-hydroxy acid oxidase, and a cosmetic composition (A) containing fatty acid peroxidase.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing the B-form the (S) -2-hydroxy acid oxidase, and a cosmetic composition (A) containing catalase.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing the B-form the (S) -2-hydroxy acid oxidase, and a cosmetic composition (A) containing peroxidase EC 1.11.1.7.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing the B-form the (S) -2-hydroxy acid oxidase, and a cosmetic composition (A) containing L-ascorbate peroxidase.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing secondary alcohol oxidase EC 1.1.3.18, and a cosmetic composition (A) containing Fettsäureperoxidase.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing secondary alcohol oxidase EC 1.1.3.18, and a cosmetic composition (A) containing Catalase.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing secondary alcohol oxidase EC 1.1.3.18, and a cosmetic composition (A) containing Peroxidase EC 1.11.1.7.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing secondary alcohol oxidase EC 1.1.3.18, and a cosmetic composition (A) containing L-ascorbate peroxidase.

Further skin-whitening agents which are particularly preferred according to the invention comprise a cosmetic Composition (B) containing N-acylhexosamine oxidase, and a cosmetic composition (A) containing fatty acid peroxidase.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing N-acylhexosamine oxidase, and a cosmetic composition (A) containing catalase.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing N-acylhexosamine oxidase, and a cosmetic composition (A) containing peroxidase EC 1.11.1.7.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing polyvinyl alcohol oxidase EC 1.1.3.30, and a cosmetic composition (A) containing Fettsäureperoxidase.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing polyvinyl alcohol oxidase EC 1.1.3.30, and a cosmetic composition (A) containing Catalase.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing polyvinyl alcohol oxidase EC 1.1.3.30, and a cosmetic composition (A) containing Peroxidase EC 1.11.1.7.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing polyvinyl alcohol oxidase EC 1.1.3.30, and a cosmetic composition (A) containing L-ascorbate peroxidase.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing vanillyl alcohol oxidase EC 1.1.3.38, and a cosmetic composition (A) containing Fettsäureperoxidase.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing vanillyl alcohol oxidase EC 1.1.3.38, and a cosmetic composition (A) containing Catalase.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing vanillyl alcohol oxidase EC 1.1.3.38, and a cosmetic composition (A) containing Peroxidase EC 1.11.1.7.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing vanillyl alcohol oxidase EC 1.1.3.38, and a cosmetic composition (A) containing L-ascorbate peroxidase.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing vanillyl alcohol oxidase EC 1.1.3.38, and a cosmetic composition (A) containing Lignin peroxidase.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing nucleoside oxidase, and a cosmetic composition (A) containing fatty acid peroxidase.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing nucleoside oxidase, and a cosmetic composition (A) containing catalase.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing nucleoside oxidase, and a cosmetic composition (A) containing peroxidase EC 1.11.1.7.

Further skin-whitening agents which are particularly preferred according to the invention comprise a cosmetic Composition (B) containing nucleoside oxidase, and a cosmetic composition (A) containing L-ascorbate peroxidase.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing D-mannitol oxidase EC 1.1.3.40, and a cosmetic composition (A) containing Fettsäureperoxidase.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing D-mannitol oxidase EC 1.1.3.40, and a cosmetic composition (A) containing Catalase.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing D-mannitol oxidase EC 1.1.3.40, and a cosmetic composition (A) containing Peroxidase EC 1.11.1.7.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing D-mannitol oxidase EC 1.1.3.40, and a cosmetic composition (A) containing L-ascorbate peroxidase.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing aryl aldehyde oxidase EC 1.2.3.9, and a cosmetic composition (A) containing Catalase.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing aryl aldehyde oxidase EC 1.2.3.9, and a cosmetic composition (A) containing Peroxidase EC 1.11.1.7.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing aryl aldehyde oxidase EC 1.2.3.9, and a cosmetic composition (A) containing L-ascorbate peroxidase.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing L-galactonolactone oxidase EC 1.3.3.12, and a cosmetic composition (A) containing Catalase.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing L-galactonolactone oxidase EC 1.3.3.12, and a cosmetic composition (A) containing Peroxidase EC 1.11.1.7.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing L-amino acid oxidase EC 1.4.3.2, and a cosmetic composition (A) containing Fettsäureperoxidase.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing L-amino acid oxidase EC 1.4.3.2, and a cosmetic composition (A) containing Catalase.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing L-amino acid oxidase EC 1.4.3.2, and a cosmetic composition (A) containing Peroxidase EC 1.11.1.7.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing L-amino acid oxidase EC 1.4.3.2, and a cosmetic composition (A) containing L-ascorbate peroxidase.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing pyridoxal 5'-phosphate synthase EC 1.4.3.5, and a cosmetic composition (A) containing Fettsäureperoxidase.

Further skin-whitening agents which are particularly preferred according to the invention comprise a cosmetic Composition (B) containing pyridoxal-5'-phosphate synthase EC 1.4.3.5, and a cosmetic composition (A) containing catalase.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing pyridoxal 5'-phosphate synthase EC 1.4.3.5, and a cosmetic composition (A) containing Peroxidase EC 1.11.1.7.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing pyridoxal 5'-phosphate synthase EC 1.4.3.5, and a cosmetic composition (A) containing L-ascorbate peroxidase.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing ethanolamine oxidase, and a cosmetic composition (A) containing fatty acid peroxidase.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing ethanolamine oxidase, and a cosmetic composition (A) containing catalase.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing ethanolamine oxidase, and a cosmetic composition (A) containing peroxidase EC 1.11.1.7.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing putrescine oxidase, and a cosmetic composition (A) containing fatty acid peroxidase.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing putrescine oxidase, and a cosmetic composition (A) containing catalase.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing putrescine oxidase, and a cosmetic composition (A) containing peroxidase EC 1.11.1.7.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing L-glutamate oxidase EC 1.4.3.11, and a cosmetic composition (A) containing Fettsäureperoxidase.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing L-glutamate oxidase EC 1.4.3.11, and a cosmetic composition (A) containing Catalase.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing L-glutamate oxidase EC 1.4.3.11, and a cosmetic composition (A) containing Peroxidase EC 1.11.1.7.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing L-glutamate oxidase EC 1.4.3.11, and a cosmetic composition (A) containing L-ascorbate peroxidase.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing protein lysine 6-oxidase EC 1.4.3.13, and a cosmetic composition (A) containing Fettsäureperoxidase.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing protein lysine 6-oxidase EC 1.4.3.13, and a cosmetic composition (A) containing Catalase.

Further skin-whitening agents which are particularly preferred according to the invention comprise a cosmetic Composition (B) containing protein lysine 6-oxidase EC 1.4.3.13, and a cosmetic composition (A) containing peroxidase EC 1.11.1.7.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing protein lysine 6-oxidase EC 1.4.3.13, and a cosmetic composition (A) containing L-ascorbate peroxidase.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing L-lysine oxidase EC 1.4.3.14, and a cosmetic composition (A) containing Fettsäureperoxidase.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing L-lysine oxidase EC 1.4.3.14, and a cosmetic composition (A) containing Catalase.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing L-lysine oxidase EC 1.4.3.14, and a cosmetic composition (A) containing Peroxidase EC 1.11.1.7.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing L-lysine oxidase EC 1.4.3.14, and a cosmetic composition (A) containing L-ascorbate peroxidase.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing L-aspartate oxidase EC 1.4.3.16, and a cosmetic composition (A) containing Fettsäureperoxidase.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing L-aspartate oxidase EC 1.4.3.16, and a cosmetic composition (A) containing Catalase.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing L-aspartate oxidase EC 1.4.3.16, and a cosmetic composition (A) containing Peroxidase EC 1.11.1.7.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing glycine oxidase EC 1.4.3.19, and a cosmetic composition (A) containing Fettsäureperoxidase.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing glycine oxidase EC 1.4.3.19, and a cosmetic composition (A) containing Catalase.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing glycine oxidase EC 1.4.3.19, and a cosmetic composition (A) containing Peroxidase EC 1.11.1.7.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing L-lysine 6-oxidase EC 1.4.3.20, and a cosmetic composition (A) containing Fettsäureperoxidase.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing L-lysine 6-oxidase EC 1.4.3.20, and a cosmetic composition (A) containing Catalase.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing L-lysine 6-oxidase EC 1.4.3.20, and a cosmetic composition (A) containing Peroxidase EC 1.11.1.7.

Further skin-whitening agents which are particularly preferred according to the invention comprise a cosmetic Composition (B) containing sarcosine oxidase EC 1.5.3.1, and a cosmetic composition (A) containing fatty acid peroxidase.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing sarcosine oxidase EC 1.5.3.1, and a cosmetic composition (A) containing Catalase.

Further According to the invention, particularly preferred skin lightening agents include a cosmetic composition (B) containing sarcosine oxidase EC 1.5.3.1, and a cosmetic composition (A) containing Peroxidase EC 1.11.1.7.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing uricase, and a cosmetic composition (A) containing catalase.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing uricase, and a cosmetic composition (A) containing peroxidase EC 1.11.1.7.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing xanthine oxidase, and a cosmetic composition (A) containing fatty acid peroxidase.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing xanthine oxidase, and a cosmetic composition (A) containing catalase.

Further According to the invention, particularly preferred skin lightening agents comprise a cosmetic composition (B) containing xanthine oxidase, and a cosmetic composition (A) containing peroxidase EC 1.11.1.7.

pH of the compositions

One important factor for the efficiency of the invention By means of the pH of the compositions (A) and (B) and the pH which results when mixing both compositions (A) and (B). Preferred inventive Means are characterized in that the composition (A) has a pH in the range of 4 to 10, preferably in the range of 5 to 8.5, more preferably in the range 5.5 to 7.5. Further preferred agents according to the invention are characterized characterized in that the composition (B) has a pH in the range from 4 to 10, preferably from 5 to 8.5, more preferably from 5.5 to 7.5, having. Further preferred inventive Means are characterized in that the pH, which varies in Mixing of both compositions (A) and (B) sets in the range from 4 to 10, preferably from 5 to 8.5, more preferably from 5.5 to 7.5, lies.

At long last is the pH of the compositions (A) and (B) to be adjusted and the pH that results when mixing both compositions (A) and (B) depends on the choice of enzymes used and their pH optimum depends. The pH optimum can also be within an enzyme classification (enzymes with identical EC number) vary, depending on the origin of the enzyme. For example, (S) -2-hydroxyacid oxidase EC 1.1.3.15, which is available from spinach, a pH optimum at pH 8, while the (S) -2-hydroxy acid oxidase EC 1.1.3.15 from maize has a pH optimum of 7.4. katalases (EC 1.11.1.6) are with pH optima in the range of 4 to 10.5, so over a broad pH spectrum, available from several sources.

Therefore are according to the invention pH values of 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5.0, 5.1, 5.2, 5.3, 5.4, 5.5, 5, 6 5.7, 5.8, 5.9, 6.0, 6.1, 6.2, 6.3, 6.4, 6.5, 6.6, 6.7, 6.8, 6, 9 7.0, 7.1, 7.2, 7.3, 7.4, 7.5, 7.6, 7.7, 7.8, 7.9, 8.0, 8.1, 8, 2, 8.3 and 8.4 are particularly preferred.

Cosmetically compatible carrier

The compositions (A) and (B) of the agents according to the invention contain the respective enzymes in a cosmetically acceptable carrier. This cosmetic carrier is preferably aqueous, alcoholic, aq Rig-alcoholic or aqueous-glycolic. Such carriers are, for example, creams, emulsions, gels or surfactant-containing foaming solutions, such as shower gels or other preparations which are suitable for use on the skin. For the purposes of the invention, an aqueous carrier preferably contains at least 40% by weight, more preferably at least 50% by weight, of water.

In the context of the present invention, aqueous-alcoholic carriers are water-containing compositions containing from 3 to 70% by weight, preferably from 10 to 60% by weight, particularly preferably from 15 to 40% by weight, of a monohydric C ₂ -C ₄ - Alcohol, especially ethanol, to understand.

In the context of the present invention, aqueous-glycolic carriers are water-containing compositions containing 3 to 70% by weight, preferably 10 to 60% by weight, more preferably 15 to 40% by weight, of at least one di- or polyvalent C ₂ C ₉ alcohol or at least one water-soluble polyethylene glycol having 3-20 ethylene oxide units, in particular selected from 1,2-propylene glycol, 2-methyl-1,3-propanediol, glycerol, 1,2-butylene glycol, 1,3-butylene glycol , 1,4-butylene glycol, pentylene glycols such as 1,2-pentanediol and 1,5-pentanediol, hexanediols such as 1,6-hexanediol, hexanetriols such as 1,2,6-hexanetriol, 1,2-octanediol, 1,8-octanediol , Dipropylene glycol, tripropylene glycol, diglycerol, triglycerol, PEG-3, PEG-4, PEG-6, PEG-7, PEG-8, PEG-9, PEG-10, PEG-12, PEG-14, PEG-16, PEG -18 and PEG-20 and mixtures thereof.

The agents according to the invention can additionally other organic solvents, such as methoxybutanol, Ethyl diglycol, 1,2-propylene glycol, 2-methyl-1,3-propanediol, n-propanol, n-butanol, n-butylene glycol, glycerol, diethylene glycol monoethyl ether and diethylene glycol mono-n-butyl ether. Preferred are all water-soluble organic solvents. Preferred agents are characterized in that the compositions (A) or (B) besides water, a nonaqueous solvent contain, with particularly preferred inventive Compositions (A) or (B) the non-aqueous solvent in a concentration of 0.1 to 30 wt .-%, preferably in one Concentration of 1 to 20 wt .-%, most preferably in one Concentration of 2 to 10 wt .-%, each based on the composition (A) or (B).

The Compositions (A) and (B) can be independent each other as an emulsion or as a gel.

• – anionische, synthetische Polymere;
• – kationische, synthetische Polymere;
• – natürlich vorkommende Verdickungsmittel, wie Guargums, Skleroglucangums oder Xanthangums, Gummi arabicum, Ghatti-Gummi, Karaya-Gummi, Tragant-Gummi, Carrageen-Gummi, Agar-Agar, Johannisbrotkernmehl, Pektine, Alginate, Stärke-Fraktionen und Derivate wie Amylose, Amylopektin und Dextrine, sowie Cellulosederivate, wie beispielsweise Methylcellulose, Carboxyalkylcellulosen und Hydroxyalkylcellulosen;
• – nichtionische, vollsynthetische Polymere, wie Polyvinylalkohol oder Polyvinylpyrrolidinon; sowie
• – anorganische Verdickungsmittel, insbesondere Schichtsilikate wie beispielsweise Bentonit, besonders Smektite, wie Montmorillonit oder Hectorit.

The ready-to-use agents according to the invention may contain further auxiliaries and additives. Thus, it has proven to be advantageous for the application and the fate of the composition on the skin when the compositions (A) and (B) independently contain at least one thickener. With regard to these thickeners, there are no fundamental restrictions. Both organic and purely inorganic thickening agents can be used. Suitable thickeners are

• Anionic synthetic polymers;
• Cationic synthetic polymers;
• Naturally occurring thickening agents, such as guar gums, scleroglucangums or xanthan gums, gum arabic, ghatti gum, karaya gum, gum tragacanth, carrageenan gum, agar agar, locust bean gum, pectins, alginates, starch fractions and derivatives such as amylose, amylopectin and dextrins, and cellulose derivatives such as methylcellulose, carboxyalkylcelluloses and hydroxyalkylcelluloses;
• Nonionic, fully synthetic polymers, such as polyvinyl alcohol or polyvinylpyrrolidinone; such as
• Inorganic thickening agents, in particular phyllosilicates, for example bentonite, especially smectites, such as montmorillonite or hectorite.

Preferably the agents are used as flowable preparations provided. "Flowable" in the The meaning of the present application are compositions which are pourable and have viscosities of 10 mPa.s to to 250,000 mPas (20 ° C). The Viscosity can be determined by standard methods (For example Brookfield viscometer LVT-II at 20 rpm and 20 ° C, spindle 3) are measured and is preferably in the range of 50 to 50,000 mPa · s. Preferred agents have Viscosities from 1000 to 30,000 mPas, with values of 5000 to 20,000 mPas are particularly preferred.

The agents according to the invention can continue contain additional active ingredients, auxiliaries and additives.

Preferred agents according to the invention comprise in at least one of the compositions (A) or (B) independently of one another at least one emulsifier or at least one surfactant, surface-active substances being referred to as surfactants or as emulsifiers, depending on the field of use ionic, cationic, zwitterionic, amphoteric and nonionic surfactants and emulsifiers.

According to the invention preferred Means are characterized in that the agent in at least one of the compositions (A) or (B) at least one anionic Contains surfactant. Preferred anionic surfactants are fatty acids, Alkyl sulfates, alkyl ether sulfates and ether carboxylic acids with 10 to 20 carbon atoms in the alkyl group and up to 16 glycol ether groups in the molecule. The anionic surfactants are in a total of 0.1 to 20 wt .-%, preferably 0.5 to 10 wt .-% and very particularly preferably from 1 to 3 wt .-%, each based on the total weight of the composition (A) or (B).

Further According to the invention preferred means are characterized characterized in that the agent is in at least one of the compositions (A) or (B) contains at least one zwitterionic surfactant. Particularly suitable zwitterionic surfactants are the so-called Betaines and N-alkyl-N, N-dimethylammonium glycinates, N-acylaminopropyl-N, N-dimethylammonium glycinates, and 2-alkyl-3-carboxymethyl-3-hydroxyethylimidazolines. A preferred one zwitterionic surfactant is that under the INCI name Cocamidopropyl Betaine known fatty acid amide derivative.

Further agents preferred according to the invention are characterized in that the agent in at least one of the compositions (A) or (B) contains at least one amphoteric surfactant. Examples of suitable amphoteric surfactants are N-alkylglycines, N-alkylpropionic acids, N-alkylaminobutyric acids, N-alkyliminodipropionic acids, N-hydroxyethyl-N-alkylamidopropylglycines, N-alkyltaurines, N-alkylsarcosines, 2-alkylaminopropionic acids and alkylaminoacetic acids. Particularly preferred amphoteric surfactants are N-cocoalkylaminopropionate, cocoacylaminoethylaminopropionate and C ₁₂ -C ₁₈ acylsarcosine.

Further According to the invention preferred means are characterized characterized in that the agent is in at least one of the compositions (A) or (B) at least one nonionic surfactant Contains substance. As preferred nonionic surfactants have alkylpolyglycosides and alkylene oxide addition products saturated linear fatty alcohols and fatty acids with in each case 2 to 30 mol of ethylene oxide per mole of fatty alcohol or fatty acid proved. Preparations with excellent properties also obtained when they are fatty acid esters as nonionic surfactants of ethoxylated glycerol.

The nonionic, zwitterionic or amphoteric surfactants are in a total amount of 0.1 to 20% by weight, preferably 0.5 to 10% by weight and most preferably from 1 to 3 wt .-%, each based on the total weight of the composition (A) or (B).

To increase the caring effect, preferred agents according to the invention may further comprise at least one fatty substance. Preferred fatty substances are vegetable oils, such as sunflower oil, olive oil, soybean oil, rapeseed oil, almond oil, jojoba oil, orange oil, wheat germ oil, peach kernel oil and the liquid portions of coconut oil, liquid paraffin oils, isoparaffin oils and synthetic hydrocarbons, di-n-alkyl ethers having a total of 12 to 36 C atoms, z. B. di-n-octyl ether and n-hexyl n-octyl ether, fatty acids, especially linear and / or branched, saturated and / or unsaturated C _8-30 fatty acids, fatty alcohols, particularly saturated, mono- or polyunsaturated, branched or unbranched fatty alcohols having 6-30 carbon atoms, ester oils, ie esters of C ₆ - ₃₀ fatty acids with C _2-30 fatty alcohols, hydroxycarboxylic acid alkyl esters, the full esters of glycolic acid, lactic acid, malic acid, tartaric acid or citric acid are preferred, dicarboxylic acid esters such as di- n-butyl adipate and diol esters such as ethylene glycol dioleate or propylene glycol di (2-ethylhexanoate), symmetrical, unsymmetrical or cyclic esters of carbonic acid with fatty alcohols, eg. As glycerol carbonate or dicaprylyl (Cetiol ^® CC), mono, - di- and tri-fatty acid esters of saturated and / or unsaturated linear and / or branched fatty acids with glycerol waxes, especially insect waxes, plant waxes, fruit waxes, ozokerite, microwaxes, ceresin, paraffin waxes, triglycerides saturated and optionally hydroxylated C _16-30 fatty acids, e.g. Hardened triglyceride fats, silicone compounds selected from decamethylcyclopentasiloxane, dodecamethylcyclohexasiloxane, and silicone polymers, which may, if desired, be crosslinked, e.g. As polydialkylsiloxanes, polyalkylarylsiloxanes, ethoxylated polydialkylsiloxanes and polydialkylsiloxanes containing amine and / or hydroxyl groups.

The Fatty substances are in a total amount of 0.1-50 wt .-%, preferably 0.1-20% by weight and more preferably 0.1-15 Wt .-%, each based on the total weight of the composition (A) or (B).

The compositions of the invention may contain other active ingredients, excipients and additives, for example vitamins, provitamins and vitamin precursors from groups A, B, C, E and F, allantoin, bisabolol and anti oxidants.

One The aim is to formulate enzyme-containing compositions in it, the enzymes contained especially during storage to stabilize. This includes protection against various unfavorable Understood influences, such as denaturation or decay due to physical influences or oxidation or also the proteolytic cleavage of the enzymes. This can be done by the construction of physical barriers, for example by encapsulation enzymes in special enzyme granules, embedded in encapsulating substances or by adsorption of the enzymes on carriers. For Chemical protection in the art are polyols, in particular Glycerol and 1,2-propylene glycol, established as protease inhibitors. Examples for further enzyme stabilizers are calcium compounds, for example, calcium chloride, calcium lactate or calcium acetate.

In general, the above-mentioned enzymes, both the H ₂ O ₂ -producing enzymes and the peroxidases of EC 1.11.1.x, can be inhibited by various cosmetic additives and excipients in their effectiveness. The presence of corresponding inhibiting agents in the skin lightening agents according to the invention should therefore be avoided.

A Enzyme inhibition according to the invention is present when the presence the enzyme-inhibiting component causes a composition, comprising enzyme and enzyme inhibiting component, after storage has a lower enzymatic activity in comparison to a control composition, differing only by the absence the enzyme inhibiting component is different from the composition. After storage, a composition according to the invention Therefore, a lower residual activity of the enzyme in comparison to the control composition, wherein composition and control composition the same initial enzymatic activity at the start of storage both compositions in the same way treated, in particular as regards the conditions of storage and the determination of enzyme activity. Increasingly preferred storage takes place for at least 24 hours, 48 hours, 72 hours, 5 days, 1 week, 2 weeks, 3 weeks or 4 weeks. Further Preferably, the storage takes place at a temperature of 20 ° C, 25 ° C or 30 ° C. For example, when using must of peroxidase of EC 1.11.1.7, note that - in Dependence on the origin of peroxidase - one whole range of cosmetically suitable additives, such as cysteine, a number of sugars, such as D-glucose and D-fructose, continue EDTA, ethanol, isopropanol, linoleic acid, magnesium sulfate, n-propyl gallate, oleic acid, Quercetin or polysorbate-80, acting as an inhibitor of peroxidase can.

Especially To avoid are inhibiting components that inhibit the enzyme activity of 30% and more, especially 50% and more.

The skin lightening agents of the invention may be packaged in known prior art two-chamber dispensers. An inventively particularly preferred dispenser is a pump dispenser, with two separate chambers analogous to that in the patent US 4875604 disclosed pump dispenser is constructed. Pump dispenser analogous to US 4875604 allow a nearly airtight and thus very storage-stable packaging of the content. In addition to propellant-free pump or squeeze dispensers, the skin whitening agents according to the invention can also be metered with a propellant, such as air. Such preferred packages are for example in the patent US 5635469 Examples XA, XB and XC. In addition, the skin lightening agents according to the invention can also be metered with a blowing agent, in particular with a compressed or liquefied propellant. When dosing with a compressed or liquefied propellant gas, a bag-in-can packaging is recommended in which the skin-whitening agent according to the invention is physically separated from the propellant gas.

• – in einem ersten Container eine kosmetische Zusammensetzung (A), enthaltend eine zur Hautaufhellung wirksame Menge mindestens einer Peroxidase der Enzymklasse mit der Enzyme Commission Number (EC) 1.11.1.x mit x = 1–3 und 5–16 sowie mindestens ein zur Hautpflege geeignetes Substrat für ein H₂O₂ produzierendes Enzym, das in dem zweiten Container enthalten ist,
• – in einem zweiten Container eine kosmetische Zusammensetzung (B), enthaltend eine zur Hautaufhellung wirksame Menge mindestens eines H₂O₂ produzierenden Enzyms sowie – sofern erforderlich – mindestens ein zur Hautpflege geeignetes Substrat für die Peroxidase der Enzymklasse mit der Enzyme Commission Number (EC) 1.11.1.x mit x = 1–3 und 5–16, die in dem ersten Container enthalten ist,

zur Aufhellung der Haut und/oder zur Aufhellung von Altersflecken oder Sommersprossen, wobei das Mittel frei ist von Substanzen, die nicht zur Hautpflege geeignet sind.A further subject matter of the present application is the non-therapeutic, cosmetic use of an agent which is in the form of a multi-component packaging unit (kit-of-parts) comprising

• In a first container, a cosmetic composition (A) comprising an effective skin lightening amount of at least one peroxidase enzyme class with the Enzyme Commission Number (EC) 1.11.1.x with x = 1-3 and 5-16 and at least one of Skin care suitable substrate for an H ₂ O ₂ producing enzyme contained in the second container,
• In a second container a cosmetic composition (B) containing an effective skin lightening amount of at least one H ₂ O ₂ -producing enzyme and - if necessary - at least one suitable for skin care substrate for the peroxidase of the enzyme class with the Enzyme Commission Number (EC) 1.11.1.x with x = 1-3 and 5-16, which is contained in the first container,

for lightening the skin and / or for lightening age spots or freckles, wherein the agent is free of substances that are not suitable for skin care.

In terms of further preferred embodiments of the invention Use applies mutatis mutandis to the invention Means said.

A further subject of the present application is a non-therapeutic, cosmetic method for lightening the skin and / or for lightening age spots or freckles, comprising a cosmetic composition (A) containing an effective skin lightening amount of at least one peroxidase of the enzyme class with the Enzyme Commission Number (EC) 1.11.1.x where x = 1-3 and 5-16 and at least one skin care substrate for an H ₂ O ₂ -producing enzyme contained in a second cosmetic composition (B), and a cosmetic composition (B) containing an effective skin lightening amount of at least one H ₂ O ₂ -producing enzyme and - if necessary - at least one suitable for skin care substrate for the peroxidase enzyme class with the Enzyme Commission Number (EC) 1.11.1.x with x = 1-3 and 5-16 contained in the first composition (A) mixed together and applied to the skin The agent is free of substances that are not suitable for skin care.

Prefers are the skin lightening agents of the invention free of substances not suitable for skin care. Especially preferred are the skin lightening agents of the invention free of oxidative hair dyes and their dye precursors as well as direct pulling hair dyes.

in the It is obvious that the meaning of the present invention the agent according to the invention immediately after to the mixing of the compositions (A) and (B) with each other or even during the mixing of the compositions (A) and (B) are applied to each other on the skin.

In terms of further preferred embodiments of the invention Method mutatis mutandis applies to the invention Means said.

The The following examples are intended to illustrate the subject matter of the invention, without limiting it to it.

Example 1a: skin lightening agent comprising

Composition (A) Catalase (EC 1.11.1.6, from Escherischia coli) 200 catalase units sodium pyruvate 0.1% by weight 1,3-butanediol 5.0% by weight 1,6-hexanediol 5.0% by weight phenoxyethanol 0.5% by weight Tego Carbomer 140 1.0% by weight NaOH beads ad pH 6 water ad 100.0% by weight Composition (B) Pyruvate oxidase (EC 1.2.3.3) 200 oxidase units 1,3-butanediol 5.0% by weight 1,6-hexanediol 5.0% by weight phenoxyethanol 0.5% by weight Tego Carbomer 140 1.0% by weight NaOH beads ad pH 6 water ad 100.0% by weight

Example 1b: skin lightening agent comprising

Composition (A) Peroxidase (EC 1.11.1.7, from Gossypium hirsutum) 300 peroxidase units sodium pyruvate 0.1% by weight 1,3-butanediol 5.0% by weight 1,6-hexanediol 5.0% by weight phenoxyethanol 0.5% by weight Tego Carbomer 140 1.0% by weight NaOH beads ad pH 6 water ad 100.0% by weight Composition (B) Pyruvate oxidase (EC 1.2.3.3) 300 oxidase units 1,3-butanediol 5.0% by weight 1,6-hexanediol 5.0% by weight phenoxyethanol 0.5% by weight Tego Carbomer 140 1.0% by weight NaOH beads ad pH 6 water ad 100.0% by weight

Example 1c: skin lightening agent comprising

Composition (A) L-ascorbate peroxidase (from radish roots, Raphanus sativus) 200 peroxidase units sodium pyruvate 0.1% by weight 1,3-butanediol 5.0% by weight 1,6-hexanediol 5.0% by weight phenoxyethanol 0.5% by weight Tego Carbomer 140 1.0% by weight NaOH beads ad pH 6 water ad 100.0% by weight Composition (B) Pyruvate oxidase (EC 1.2.3.3) 200 oxidase units Magnesium L-ascorbate 0.1% by weight 1,3-butanediol 5.0% by weight 1,6-hexanediol 5.0% by weight phenoxyethanol 0.5% by weight Tego Carbomer 140 1.0% by weight NaOH beads ad pH 6 water ad 100.0% by weight Plantacare 2000 UP: DECYL GLUCOSIDE (53% by weight), AQUA (WATER) (ex Cognis)

The Compositions (A) and (B) are in a two-chamber dispenser physically separated from each other and are used by pumping with two separate outlet openings in the Weight ratio 1: 1 taken simultaneously and at Apply to the skin by triturating mixed together.

Example 2: Skin lightening agent comprising

Composition (A) L-ascorbate peroxidase (EC 1.11.1.11, from Pisum sativum) 300 peroxidase units D-alanine 0.1% by weight 1,3-butanediol 5.0% by weight 1,6-hexanediol 5.0% by weight phenoxyethanol 0.5% by weight Tego Carbomer 140 1.0% by weight NaOH beads ad pH 7 water ad 100.0% by weight Composition (B) Glycine oxidase (EC 1.4.3.19) 200 oxidase units Sodium L-ascorbate 0.1% by weight 1,3-butanediol 5.0% by weight 1,6-hexanediol 5.0% by weight phenoxyethanol 0.5% by weight Tego Carbomer 140 1.0% by weight NaOH beads ad pH 7 water ad 100.0% by weight

Example 3: skin lightening agent comprising

Composition (A) L-ascorbate peroxidase (EC 1.11.1.11, from radish roots, Raphanus sativus) 200 units D-glucose 0.9% by weight 1,3-butanediol 5.0% by weight 1,6-hexanediol 5.0% by weight phenoxyethanol 0.5% by weight Tego Carbomer 140 1.0% by weight NaOH beads ad pH 6 water ad 100.0% by weight Composition (B) Glucose oxidase (EC 1.1.3.4) 200 units Magnesium L-ascorbate 0.8% by weight 1,3-butanediol 5.0% by weight 1,6-hexanediol 5.0% by weight phenoxyethanol 0.5% by weight Tego Carbomer 140 1.0% by weight NaOH beads ad pH 6 water ad 100.0% by weight

Example 4: Skin lightening agent comprising

Composition (A) Peroxidase (EC 1.11.1.7, from Gossypium hirsutum) 400 units beta-sitosterol 0.3% by weight Plantacare 2000 UP 0.2% by weight 1,3-butanediol 5.0% by weight 1,6-hexanediol 5.0% by weight phenoxyethanol 0.5% by weight Tego Carbomer 140 1.0% by weight NaOH beads ad pH 6 water ad 100.0% by weight Composition (B) Cholesterol oxidase (EC 1.1.3.6) 400 units 1,3-butanediol 5.0% by weight 1,6-hexanediol 5.0% by weight phenoxyethanol 0.5% by weight Tego Carbomer 140 1.0% by weight NaOH beads ad pH 6 water ad 100.0% by weight

Example 5: Skin whitening agent comprising

Composition (A) L-ascorbate peroxidase (EC 1.11.1.11, from Pisum sativum) 300 units L-sorbose 1.0% by weight 1,3-butanediol 5.0% by weight 1,6-hexanediol 5.0% by weight phenoxyethanol 0.5% by weight Tego Carbomer 140 1.0% by weight NaOH beads ad pH 5.7 water ad 100.0% by weight Composition (B) L-sorbose oxidase (EC 1.1.3.11) 300 units Magnesium L-ascorbate 0.8% by weight 1,3-butanediol 5.0% by weight 1,6-hexanediol 5.0% by weight phenoxyethanol 0.5% by weight Tego Carbomer 140 1.0% by weight NaOH beads ad pH 5.7 water ad 100.0% by weight

Example 6: Skin lightening agent comprising

Composition (A) Fatty acid peroxidase (EC 1.11.1.3, from Pisum sativum) 200 units glycolic 0.6% by weight 1,3-butanediol 5.0% by weight 1,6-hexanediol 5.0% by weight phenoxyethanol 0.5% by weight Tego Carbomer 140 1.0% by weight NaOH beads ad pH 7.3 water ad 100.0% by weight Composition (B) (S) -2-hydroxy acid oxidase (EC 1.1.3.15, A-form, from Zea mays) 200 units palmitic 0.8% by weight Plantacare 2000 UP 0.2% by weight 1,3-butanediol 5.0% by weight 1,6-hexanediol 5.0% by weight phenoxyethanol 0.5% by weight Tego Carbomer 140 1.0% by weight NaOH beads ad pH 7.3 water ad 100.0% by weight

Example 7: skin lightening agent comprising

Composition (A) Fatty acid peroxidase (EC 1.11.1.3, from Pisum sativum) 300 units 2-methyl-2,4-pentanediol 2.0% by weight 1,3-butanediol 5.0% by weight 1,6-hexanediol 5.0% by weight phenoxyethanol 0.5% by weight Tego Carbomer 140 1.0% by weight NaOH beads ad pH 7.3 water ad 100.0% by weight Composition (B) Secondary alcohol oxidase (EC 1.1.3.18 from sphingopyxis) 300 units palmitic 0.8% by weight Plantacare 2000 UP 0.2% by weight 1,3-butanediol 5.0% by weight 1,6-hexanediol 5.0% by weight phenoxyethanol 0.5% by weight Tego Carbomer 140 1.0% by weight NaOH beads ad pH 7.3 water ad 100.0% by weight

Example 8: Skin lightening agent comprising

Composition (A) Peroxidase (EC 1.11.1.7, from Helianthus annuus roots) 200 units N-acetyl-D-glucosamine 0.8% by weight 1,3-butanediol 5.0% by weight 1,6-hexanediol 5.0% by weight phenoxyethanol 0.5% by weight Tego Carbomer 140 1.0% by weight NaOH beads ad pH 8 water ad 100.0% by weight Composition (B) N-acylhexosamine oxidase (EC 1.1.3.29 from Pseudomonas) 200 units 1,3-butanediol 5.0% by weight 1,6-hexanediol 5.0% by weight phenoxyethanol 0.5% by weight Tego Carbomer 140 1.0% by weight NaOH beads ad pH 8 water ad 100.0% by weight

Example 9: Skin lightening agent comprising

Composition (A) Fatty acid peroxidase (EC 1.11.1.3, from Pisum sativum) 400 units Polyvinyl alcohol (Mw = 49000 Da) 0.2% by weight 1,3-butanediol 5.0% by weight 1,6-hexanediol 5.0% by weight phenoxyethanol 0.5% by weight Tego Carbomer 140 1.0% by weight NaOH beads ad pH 7.2 water ad 100.0% by weight Composition (B) Polyvinyl alcohol oxidase (EC 1.1.3.30 from Sphingopyxis) 400 units palmitic 1.0% by weight Plantacare 2000 UP 1.0% by weight 1,3-butanediol 5.0% by weight 1,6-hexanediol 5.0% by weight phenoxyethanol 0.5% by weight Tego Carbomer 140 1.0% by weight NaOH beads ad pH 7.2 water ad 100.0% by weight

Example 10: skin lightening agent comprising

Composition (A) Fatty acid peroxidase (EC 1.11.1.3, from Pisum sativum) 200 units D-mannitol 1.0% by weight 1,6-hexanediol 10.0% by weight phenoxyethanol 0.5% by weight Tego Carbomer 140 1.0% by weight NaOH beads ad pH 7.4 water ad 100.0% by weight Composition (B) D-mannitol oxidase (EC 1.1.3.40 from Helix aspersa) 200 units palmitic 1.0% by weight Plantacare 2000 UP 1.0% by weight 1,6-hexanediol 10.0% by weight phenoxyethanol 0.5% by weight Tego Carbomer 140 1.0% by weight NaOH beads ad pH 7.4 water ad 100.0% by weight

Example 11: Skin lightening agent comprising

Composition (A) Fatty acid peroxidase (EC 1.11.1.3, from Pisum sativum) 300 units L-methionine 0.2% by weight 1,6-hexanediol 10.0% by weight phenoxyethanol 0.5% by weight Tego Carbomer 140 1.0% by weight NaOH beads ad pH 7.6 water ad 100.0% by weight Composition (B) L-amino acid oxidase (EC 1.4.3.2 from Crotalus adamanteus toxin) 300 units palmitic 1.0% by weight Plantacare 2000 UP 1.0% by weight 1,6-hexanediol 10.0% by weight phenoxyethanol 0.5% by weight Tego Carbomer 140 1.0% by weight NaOH beads ad pH 7.6 water ad 100.0% by weight

Example 12: skin lightening agent comprising

Composition (A) Peroxidase (EC 1.11.1.7, from Helianthus annuus roots) 200 units putrescine 0.1% by weight 1,3-butanediol 5.0% by weight 1,6-hexanediol 5.0% by weight phenoxyethanol 0.5% by weight Tego Carbomer 140 1.0% by weight NaOH beads ad pH 8 water ad 100.0% by weight Composition (B) Putrescine oxidase (EC 1.4.3.10 from Rhodococcus erythropolis) 200 units 1,3-butanediol 5.0% by weight 1,6-hexanediol 5.0% by weight phenoxyethanol 0.5% by weight Tego Carbomer 140 1.0% by weight NaOH beads ad pH 8 water ad 100.0% by weight

Example 13: Skin lightening agent comprising

Composition (A) L-ascorbate peroxidase (EC 1.11.1.11, from radish roots, Raphanus sativus) 300 units Sodium L-glutamate 0.2% by weight 1,3-butanediol 5.0% by weight 1,6-hexanediol 5.0% by weight phenoxyethanol 0.5% by weight Tego Carbomer 140 1.0% by weight NaOH beads ad pH 6 Water ad 100.0% by weight Composition (B) L-glutamate oxidase (EC 1.4.3.11 from Streptomyces) 300 units Magnesium L-ascorbate 0.2% by weight 1,3-butanediol 5.0% by weight 1,6-hexanediol 5.0% by weight phenoxyethanol 0.5% by weight Tego Carbomer 140 1.0% by weight NaOH beads ad pH 6 water ad 100.0% by weight

Example 14: skin lightening agent comprising

Composition (A) Catalase (EC 1.11.1.6, from Escherischia coli) 400 units sodium glycinate 0.5% by weight 1,3-butanediol 5.0% by weight 1,6-hexanediol 5.0% by weight phenoxyethanol 0.5% by weight Tego Carbomer 140 1.0% by weight NaOH beads ad pH 8.0 water ad 100.0% by weight Composition (B) Glycine oxidase (EC 1.4.3.19 from Bacillus subtilis) 400 units 1,3-butanediol 5.0% by weight 1,6-hexanediol 5.0% by weight phenoxyethanol 0.5% by weight Tego Carbomer 140 1.0% by weight NaOH beads ad pH 8.0 water ad 100.0% by weight

Example 15: Skin lightening agent comprising

Composition (A) Catalase (EC 1.11.1.6, from Escherischia coli) 200 units sodium sarcosinate 0.5% by weight 1,3-butanediol 5.0% by weight 1,6-hexanediol 5.0% by weight phenoxyethanol 0.5% by weight Tego Carbomer 140 1.0% by weight NaOH beads ad pH 8.0 water ad 100.0% by weight

Composition (B) Sarcosine oxidase (EC 1.5.3.1 from Corynebacterium) 200 units 1,3-butanediol 5.0% by weight 1,6-hexanediol 5.0% by weight phenoxyethanol 0.5% by weight Tego Carbomer 140 1.0% by weight NaOH beads ad pH 8.0 water ad 100.0% by weight

Example 16: Skin lightening agent comprising

Composition (A) Peroxidase (EC 1.11.1.7, from Helianthus annuus roots) 300 units uric acid 0.1% by weight glycerin 5.0% by weight 1,6-hexanediol 5.0% by weight phenoxyethanol 0.5% by weight Tego Carbomer 140 1.0% by weight NaOH beads ad pH 8.0 water ad 100.0% by weight Composition (B) Uricase (EC 1.7.3.3, from Aspergillus flavus) 300 units 1,3-butanediol 5.0% by weight 1,6-hexanediol 5.0% by weight phenoxyethanol 0.5% by weight Tego Carbomer 140 1.0% by weight NaOH beads ad pH 8.0 water ad 100.0% by weight

Example 17: skin lightening agent comprising

Composition (A) Fatty acid peroxidase (EC 1.11.1.3, from Pisum sativum) 400 units caffeine 0.3% by weight glycerin 5.0% by weight 1,6-hexanediol 5.0% by weight phenoxyethanol 0.5% by weight Tego Carbomer 140 1.0% by weight NaOH beads ad pH 7.4 water ad 100.0% by weight Composition (B) Xanthine oxidase (EC 1.17.3.2) 400 units palmitic 0.8% by weight Plantacare 2000 UP 0.2% by weight 1,3-butanediol 5.0% by weight 1,6-hexanediol 5.0% by weight phenoxyethanol 0.5% by weight Tego Carbomer 140 1.0% by weight NaOH beads ad pH 7.4 water ad 100.0% by weight

The Compositions (A) and (B) are in a two-chamber dispenser physically separated from each other and are used by pumping with two separate outlet openings at the same time taken in a weight ratio of 1: 1 and during application mixed with the skin by triturating.

QUOTES INCLUDE IN THE DESCRIPTION

This list The documents listed by the applicant have been automated generated and is solely for better information recorded by the reader. The list is not part of the German Patent or utility model application. The DPMA takes over no liability for any errors or omissions.

Cited patent literature

• - WO 2004017931 A1 [0008, 0008]
• US 4875604 [0182, 0182]
• US 5635469 [0182]

Cited non-patent literature

• Gornall AG, CS Bardawill and MM David, J. Biol. Chem., 177 (1948), pp. 751-766 [0021]

Claims (10)

Cosmetic agent for the lightening of the skin and / or of age spots, which is in the form of a multi-component packaging unit (kit-of-parts), comprising a) in a first container, a cosmetic composition (A) containing an effective skin lightening amount of at least one peroxidase Enzyme class with the Enzyme Commission Number (EC) 1.11.1.x where x = 1-3 and 5-16 and at least one skin care substrate for an H ₂ O ₂ producing enzyme contained in the second container, b) in a second container, a cosmetic composition (B) containing an effective for skin lightening amount of at least one H ₂ O ₂ -producing enzyme and - if necessary - at least one suitable for skin care substrate for the peroxidase enzyme class with the Enzyme Commission Number (EC) 1.11 .1.x with x = 1-3 and 5-16 contained in the first container, the agent being free of substances not for skin care are suitable. Cosmetic composition according to claim 1, characterized in that the enzyme producing at least one H ₂ O _{2 is} selected from enzymes of EC 1.1.3.3 malate oxidase. 1.1.3.4 glucose oxidase 1.1.3.5 hexose oxidase 1.1.3.6 cholesterol oxidase 1.1 .3.7 Aryl alcohol oxidase, veratryl alcohol oxidase 1.1.3.8 L-gulonolactone oxidase 1.1.3.9 Galactose oxidase 1.1.3.10 Pyranose oxidase 1.1.3.11 L-sorbose oxidase 1.1.3.12 Pyridoxine 4-oxidase 1.1.3.13 Alcohol oxidase, ethanol Oxidase 1.1.3.15 Glycolate Oxidase, (S) -2-Hydroxy Acid Oxidase 1.1.3.16 Ecdysone Oxidase 1.1.3.17 Choline Oxidase 1.1.3.18 Oxidase for Secondary Alcohols 1.1.3.19 4-Hydroxymandelate Oxidase (decarboxylative) 1.1.3.21 Glycerol-3-phosphate oxidase 1.1.3.23 thiamine oxidase 1.1.3.27 hydroxyphytanate oxidase 1.1.3.29 N-acylhexosamine oxidase 1.1.3.30 polyvinyl alcohol oxidase; PVA oxidase 1.1.3.37 D-arabinono-1,4-lactone oxidase 1.1.3.38 Vanillyl alcohol oxidase 1.1.3.39 Nucleoside oxidase (H ₂ O ₂ -forming) 1.1.3.40 D-Mannitol oxidase 1.1.3.41 Alditol Oxidase (xylitol oxidase) 1.2.3.1 aldehyde oxidase, quinoline oxidase 1.2.3.3 pyruvate oxidase 1.2.3.4 oxalate oxidase 1.2.3.5 glyoxylate oxidase 1.2.3.6 pyruvate oxidase 1.2.3.7 indole-3-acetaldehyde oxidase 1.2.3.9 Aryl aldehyde oxidase 1.2.3.11 Retinal oxidase 1.2.3.14 Abscisinaldehyde oxidase 1.3.3.1 Dihydroorotate oxidase 1.3.3.4 Protoporphyrinogen oxidase 1.3.3.6 Acyl-CoA oxidase 1.3.3.7 Dihydrouracil oxidase 1.3.3.8 Tetrahydroberberine oxidase 1.3.3.10 Tryptophan α, β-oxidase 1.3.3.11 Pyrroloquinoline quinone synthase, PqqC 1.3.3.12 L-galactonolactone oxidase 1.4.3.1 D-Aspartate oxidase 1.4.3.2 L-Amino Acid Oxidase 1.4.3.3 D-Amino Acid Oxidase 1.4.3.4 Monoamine Oxidase 1.4.3.5 Pyridoxal 5'-Phosphate Synthase 1.4.3.7 D-Glutamate Oxidase 1.4.3.8 Ethanolamine Oxidase 1.4.3.10 Putrescine oxidase 1.4.3.11 L-glutamate oxidase 1.4.3.12 Cyclohexylamine oxidase 1.4.3.13 Protein lysine 6-oxidase, lysine oxidase 1.4.3.14 L-lysine oxidase 1.4.3.15 D-glutamate oxidase, D- Aspartate oxidase 1.4.3.16 L-Aspartate oxidase 1.4.3.19 Glycine oxidase 1.4.3.20 L-lysine 6-oxidase 1.4.3.21 Primary amine oxidase 1.4.3.22 Diamine oxidase 1.5.3.1 Sarcosine oxidase 1.5.3.2 N- Methyl L-amino acid oxidase 1.5.3.4 N ⁶ -methyllysine oxidase 1.5.3.5 (S) -6-Hydroxynicotin oxidase 1.5.3.6 (R) -6-Hydroxynicotin oxidase 1.5.3.7 L-pipecolate oxidase 1.5. 3.10 Dimethylglycine oxidase 1.5.3.11 Polyamine oxidase 1.5.3.12 Dihydrobenzophenanthridine oxidase 1.6.3.1 NAD (P) H-oxidase 1.7.3.1 Nitroalkane oxidase 1.7.3.3 Uricase (urate oxidase) 1.7.3.5 3-Acid-nitropropanoate oxidase 1.8 .3.1 Sulfite oxidase 1.8.3.2 Thiol oxidase, sulfhydryl oxidase e 1.8.3.3 Glutathione Oxidase 1.8.3.4 Methanethiol Oxidase 1.8.3.5 Prenylcysteine Oxidase 1.10.3.5 3-Hydroxyanthranilate Oxidase 1.10.3.6 Rifamycin B Oxidase 1.15.1.1 Superoxide Dismutase 1.15.1.2 Superoxide Reductase 1.15.1.3 Superoxide Dismutase 1.17.3.2 Xanthine Oxidase 1.17.3.3 6-Hydroxynicotinate dehydrogenase 1.21.3.3 Reticulin oxidase. Cosmetic composition according to claim 1 or 2, characterized in that the at least one peroxidase of EC 1.11.1.x with x = 1-3 and 5-16 is selected from EC 1.11.1.1 NADH peroxidase, EC 1.11.1.2 NADPH- Peroxidase, EC 1.11.1.3 fatty acid peroxidase, EC 1.11.1.5 cytochrome c peroxidase, EC 1.11.1.6 catalase, EC 1.11.1.7 peroxidase (synonym: lactoperoxidase, horseradish peroxidase), EC 1.11.1.8 iodide peroxidase, EC 1.11. 1.9 Glutathione peroxidase, EC 1.11.1.10 Chloride peroxidase, EC 1.11.1.11 L-ascorbate peroxidase, EC 1.11.1.12 Phospholipid hydroperoxide glutathione peroxidase, EC 1.11.1.13 Manganese peroxidase, EC 1.11.1.14 Lignin peroxidase , EC 1.11.1.15 Peroxiredoxin and EC 1.11.1.16 Hybrid peroxidase (versatile peroxidase). Cosmetic composition according to claim 1, 2 or 3, characterized in that the at least one H ₂ O ₂ -producing enzyme is selected from enzymes of EC 1.1.3.x with x = 3-13, 15, 18, 29, 30 and 37 -41, EC 1.2.3.9, EC 1.3.3.12, EC 1.4.3.x where x = 2, 5, 8, 10, 11, 13, 14, 16, 19 and 20, EC 1.5.3.1, EC 1.7. 3.3, EC 1.15.1.1 and EC 1.17.3.2. Cosmetic composition according to claim 1, 2, 3 or 4, characterized in that the at least one peroxidase of EC 1.11.1.x with x = 1-3 and 5-16 selected is from EC 1.11.1.3, EC 1.11.1.6, EC 1.11.1.7, EC 1.11.1.11 and EC 1.11.1.14. Cosmetic composition according to claim 1, 2, 3, 4 or 5, characterized in that preferred enzyme combinations (A) and (B) are selected from - glucose oxidase and catalase - glucose oxidase and peroxidase EC 1.11.1.7 - glucose oxidase and L-ascorbate peroxidase - hexose oxidase and catalase - hexose oxidase and peroxidase EC 1.11.1.7 - hexose oxidase and L-ascorbate peroxidase - cholesterol oxidase and fatty acid peroxidase - cholesterol oxidase and catalase - cholesterol oxidase and peroxidase EC 1.11.1.7 - Cholesterol oxidase and L-ascorbate peroxidase - Aryl alcohol oxidase EC 1.1.3.7 and fatty acid peroxidase - Aryl alcohol oxidase EC 1.1.3.7 and catalase - Aryl alcohol oxidase EC 1.1.3.7 and peroxidase EC 1.11.1.7 - Aryl alcohol Oxidase EC 1.1.3.7 and L-ascorbate peroxidase - galactose oxidase and fatty acid peroxidase - galactose oxidase and catalase - galactose oxidase and peroxidase EC 1.11.1.7 - galactose oxidase and L-ascorbate peroxidase - pyran ose oxidase and fatty acid peroxidase - pyranose oxidase and catalase - pyranose oxidase and peroxidase EC 1.11.1.7 - pyranose oxidase and L-ascorbate peroxidase - pyranose oxidase and lignin peroxidase - L-sorbose oxidase and catalase - L- Sorbose oxidase and peroxidase EC 1.11.1.7 - L-sorbose oxidase and L-ascorbate peroxidase - pyridoxine 4-oxidase and fatty acid peroxidase - pyridoxine 4-oxidase and catalase - pyridoxine 4-oxidase and peroxidase EC 1.11.1.7 - Pyridoxine 4-oxidase and L-ascorbate peroxidase - Alcohol oxidase EC 1.1.3.13 and fatty acid peroxidase - Alcohol oxidase EC 1.1.3.13 and catalase - Alcohol oxidase EC 1.1.3.13 and peroxidase EC 1.11.1.7 - Alcohol oxidase EC 1.1.3.13 and L-ascorbate peroxidase - A form of (S) -2-hydroxy acid oxidase and fatty acid peroxidase - A form of (S) -2-hydroxy acid oxidase and catalase - A form of (S) - 2-hydroxyacid oxidase and peroxidase EC 1.11.1.7 - A-form of (S) -2-hydroxyacid oxidase and L-ascorbate-P eroxidase - B-form of (S) -2-hydroxyacid oxidase and fatty acid peroxidase - B-form of (S) -2-hydroxyacid oxidase and catalase - B-form of (S) -2-hydroxyacid oxidase and peroxidase EC 1.11.1.7 - B-form of (S) -2-hydroxy acid oxidase and L-ascorbate peroxidase - secondary alcohol oxidase EC 1.1.3.18 and fatty acid peroxidase - secondary alcohol oxidase EC 1.1.3.18 and catalase - secondary alcohol oxidase EC 1.1. 3.18 and peroxidase EC 1.11.1.7 - secondary alcohol oxidase EC 1.1.3.18 and L-ascorbate peroxidase - N-acylhexosamine oxidase and fatty acid peroxidase - N-acylhexosamine oxidase and catalase - N-acylhexosamine oxidase and peroxidase EC 1.11.1.7 - polyvinyl alcohol oxidase EC 1.1.3.30 and fatty acid peroxidase - polyvinyl alcohol oxidase EC 1.1.3.30 and catalase - polyvinyl alcohol oxidase EC 1.1.3.30 and peroxidase EC 1.11.1.7 - Polyvinyl alcohol oxidase EC 1.1.3.30 and L-ascorbate peroxidase - vanillyl alcohol oxidase EC 1.1.3.38 and fatty acid peroxidase - vanillyl alcohol oxidase EC 1.1.3.38 and catalase - vanillyl alcohol oxidase EC 1.1.3.38 and peroxidase EC 1.11 .1.7 - Vanillyl alcohol oxidase EC 1.1.3.38 and L-ascorbate peroxidase - Nucleoside oxidase and fatty acid peroxidase - Nucleoside oxidase and catalase - Nucleoside oxidase and peroxidase EC 1.11.1.7 - Nucleoside oxidase and L-ascorbate peroxidase - D -Mannitol oxidase EC 1.1.3.40 and fatty acid peroxidase - D-mannitol oxidase EC 1.1.3.40 and catalase - D-mannitol oxidase EC 1.1.3.40 and peroxidase EC 1.11.1.7 - D-mannitol oxidase EC 1.1.3.40 and L ascorbate -Peroxidase - pyruvate oxidase and catalase - pyruvate oxidase and peroxidase EC 1.11.1.7 - pyruvate oxidase and L-ascorbate peroxidase - aryl aldehyde oxidase EC 1.2.3.9 and fatty acid peroxidase - aryl aldehyde oxidase EC 1.2.3.9 and catalase - aryl aldehyde -Oxidase EC 1.2.3.9 and peroxidase EC 1.11.1.7 - aryl aldehyde oxidase EC 1.2.3.9 and L-ascorbate peroxidase - L-galactonolactone oxidase EC 1.3.3.12 and catalase - L-galactonolactone oxidase EC 1.3.3.12 and peroxidase EC 1.11.1.7 - L-amino acid oxidase EC 1.4.3.2 and fatty acid peroxidase - L-amino acid oxidase EC 1.4.3.2 and catalase - L-amino acid oxidase EC 1.4.3.2 and peroxidase EC 1.11.1.7 - L-amino acid oxidase EC 1.4.3.2 and L-ascorbate peroxidase - pyridoxal 5'-phosphate synthase EC 1.4.3.5 and fatty acid peroxidase - pyridoxal 5'-phosphate synthase EC 1.4.3.5 and catalase - pyridoxal 5'-phosphate synthase EC 1.4.3.5 and Peroxidase EC 1.11.1.7 - Pyridoxal 5'-Phosphate Synthase EC 1.4.3.5 and L-Ascorbate Pero xidase - ethanolamine oxidase and fatty acid peroxidase - ethanolamine oxidase and catalase - ethanolamine oxidase and peroxidase EC 1.11.1.7 - putrescine oxidase and fatty acid peroxidase - putrescine oxidase and catalase - putrescine oxidase and peroxidase EC 1.11.1.7 - L-glutamate Oxidase EC 1.4.3.11 and fatty acid peroxidase - L-glutamate oxidase EC 1.4.3.11 and catalase - L-glutamate oxidase EC 1.4.3.11 and peroxidase EC 1.11.1.7 - L-glutamate oxidase EC 1.4.3.11 and L-ascorbate Peroxidase - Protein lysine 6-oxidase EC 1.4.3.13 and fatty acid peroxidase - Protein lysine 6-oxidase EC 1.4.3.13 and catalase - Protein lysine 6-oxidase EC 1.4.3.13 and peroxidase EC 1.11.1.7 - Protein Lysine 6-oxidase EC 1.4.3.13 and L-ascorbate peroxidase - L-lysine oxidase EC 1.4.3.14 and fatty acid peroxidase - L-lysine oxidase EC 1.4.3.14 and catalase - L-lysine oxidase EC 1.4.3.14 and Peroxidase EC 1.11.1.7 - L-lysine oxidase EC 1.4.3.14 and L-ascorbate peroxidase - L-aspartate oxidase E C 1.4.3.16 and fatty acid peroxidase - L-aspartate oxidase EC 1.4.3.16 and catalase - L-aspartate oxidase EC 1.4.3.16 and peroxidase EC 1.11.1.7 - Glycine oxidase EC 1.4.3.19 and fatty acid peroxidase - glycine oxidase EC 1.4 .3.19 and catalase - glycine oxidase EC 1.4.3.19 and peroxidase EC 1.11.1.7 - L-lysine 6-oxidase EC 1.4.3.20 and fatty acid peroxidase - L-lysine 6-oxidase EC 1.4.3.20 and catalase - L-lysine 6-oxidase EC 1.4.3.20 and peroxidase EC 1.11.1.7 - sarcosine oxidase EC 1.5.3.1 and fatty acid peroxidase - sarcosine oxidase EC 1.5.3.1 and catalase - sarcosine oxidase EC 1.5.3.1 and peroxidase EC 1.11.1.7 - uricase and catalase - uricase and peroxidase EC 1.11.1.7 - xanthine oxidase and fatty acid peroxidase - xanthine oxidase and catalase - xanthine oxidase and peroxidase EC 1.11.1.7 Non-therapeutic, cosmetic use of a Agent according to one of claims 1, 2, 3, 4, 5 or 6 for lightening the skin and / or for lightening from age spots or freckles. Non-therapeutic, cosmetic method for lightening the skin and / or for lightening age spots or freckles, characterized in that a cosmetic composition (A) containing an effective skin lightening amount of at least one peroxidase enzyme class with the Enzyme Commission Number (EC) 1.11.1.x with x = 1-3 and 5-16 and at least one suitable skin care substrate for an H ₂ O ₂ -producing enzyme contained in a second cosmetic composition (B), and a cosmetic composition (B) containing at least one H ₂ O _2- producing enzyme for skin lightening and, if necessary, at least one skin care substrate for the enzyme class peroxidase having the Enzyme Commission Number (EC) 1.11.1.x with x = 1-3 and 5-16 contained in the first composition (A) are mixed together and applied to the skin, the agent being free of substances that are not suitable for skin care. A method according to claim 8, characterized in that the at least one H ₂ O ₂ -producing enzyme is selected according to claim 2. A method according to claim 8 or 9, characterized in that the at least one peroxidase of EC 1.11.1.x with x = 1-3 and 5-16 selected is out EC 1.11.1.1 NADH peroxidase, EC 1.11.1.2 NADPH peroxidase, EC 1.11.1.3 fatty acid peroxidase, EC 1.11.1.5 cytochrome c peroxidase, EC 1.11.1.6 Catalase, EC 1.11.1.7 Peroxidase (synonym: lactoperoxidase, Horseradish peroxidase) EC 1.11.1.8 iodide peroxidase, EC 1.11.1.9 glutathione peroxidase, EC 1.11.1.10 Chloride peroxidase, EC 1.11.1.11 L-ascorbate peroxidase, EC 1.11.1.12 phospholipid hydroperoxide glutathione peroxidase, EC 1.11.1.13 manganese peroxidase, EC 1.11.1.14 lignin peroxidase, EC 1.11.1.15 Peroxiredoxin and EC 1.11.1.16 Hybrid peroxidase (versatile peroxidase).