CN103103175A - 新型腈水合酶 - Google Patents

新型腈水合酶 Download PDF

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Publication number
CN103103175A
CN103103175A CN2012105742761A CN201210574276A CN103103175A CN 103103175 A CN103103175 A CN 103103175A CN 2012105742761 A CN2012105742761 A CN 2012105742761A CN 201210574276 A CN201210574276 A CN 201210574276A CN 103103175 A CN103103175 A CN 103103175A
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CN
China
Prior art keywords
sequence
sequence number
nitrile hydratase
replaced
subunit
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN2012105742761A
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English (en)
Chinese (zh)
Inventor
八卷俊文
番场伸一
的石香
伊藤洁
小林英树
田中英司
及川利洋
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsui Chemicals Inc
Mitsui Chemical Industry Co Ltd
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Mitsui Chemical Industry Co Ltd
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Publication date
Application filed by Mitsui Chemical Industry Co Ltd filed Critical Mitsui Chemical Industry Co Ltd
Publication of CN103103175A publication Critical patent/CN103103175A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/01—Hydro-lyases (4.2.1)
    • C12Y402/01084—Nitrile hydratase (4.2.1.84)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN2012105742761A 2002-12-19 2003-12-15 新型腈水合酶 Pending CN103103175A (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2002368360A JP2004194588A (ja) 2002-12-19 2002-12-19 新規なニトリルヒドラターゼ
JP368360/2002 2002-12-19
JP379280/2003 2003-11-10
JP2003379280 2003-11-10

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CNA2003801069930A Division CN1729288A (zh) 2002-12-19 2003-12-15 新型腈水合酶

Publications (1)

Publication Number Publication Date
CN103103175A true CN103103175A (zh) 2013-05-15

Family

ID=32684188

Family Applications (4)

Application Number Title Priority Date Filing Date
CN2012105742761A Pending CN103103175A (zh) 2002-12-19 2003-12-15 新型腈水合酶
CN2010101672031A Pending CN101892211A (zh) 2002-12-19 2003-12-15 新型腈水合酶
CN2012105742117A Pending CN103215242A (zh) 2002-12-19 2003-12-15 新型腈水合酶
CNA2003801069930A Pending CN1729288A (zh) 2002-12-19 2003-12-15 新型腈水合酶

Family Applications After (3)

Application Number Title Priority Date Filing Date
CN2010101672031A Pending CN101892211A (zh) 2002-12-19 2003-12-15 新型腈水合酶
CN2012105742117A Pending CN103215242A (zh) 2002-12-19 2003-12-15 新型腈水合酶
CNA2003801069930A Pending CN1729288A (zh) 2002-12-19 2003-12-15 新型腈水合酶

Country Status (7)

Country Link
US (1) US7595184B2 (enExample)
EP (1) EP1586637B1 (enExample)
JP (1) JP2004194588A (enExample)
KR (5) KR100785549B1 (enExample)
CN (4) CN103103175A (enExample)
AU (2) AU2003289338A1 (enExample)
WO (1) WO2004056990A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112322606A (zh) * 2020-11-20 2021-02-05 江南大学 一种腈水合酶突变体及其应用

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1767624B1 (en) * 2004-05-26 2013-11-06 Mitsubishi Rayon Co., Ltd. Improved nitrile hydratase
EP1842907A1 (en) 2006-04-07 2007-10-10 B.R.A.I.N. Ag A group of novel enantioselective microbial nitrile hydratases with broad substrate specificity
JP5069032B2 (ja) * 2007-04-04 2012-11-07 ダイヤニトリックス株式会社 改良型ニトリルヒドラターゼ
KR101532467B1 (ko) * 2008-11-14 2015-07-06 미쓰이 가가쿠 가부시키가이샤 니트릴 히드라타아제 변이체
JP5532618B2 (ja) * 2009-01-30 2014-06-25 三菱レイヨン株式会社 改良型ニトリルヒドラターゼ及びその製造方法
EP2426210A4 (en) * 2009-04-30 2016-12-21 Mitsui Chemicals Inc PROCESS FOR PRODUCING 3-MERCAPTOPROPIONIC ACID OR SALT THEREOF
KR20140006052A (ko) 2011-05-31 2014-01-15 미쯔비시 레이온 가부시끼가이샤 개량형 니트릴 히드라타제
CN104531654B (zh) * 2011-06-07 2018-06-12 三菱化学株式会社 改良型腈水合酶
CN102517271B (zh) * 2011-12-13 2013-04-03 清华大学 一种突变腈水合酶
EP3153582B1 (en) 2014-06-06 2020-06-17 Mitsubishi Chemical Corporation Improved nitrile hydratase
CN104725476B (zh) * 2015-03-31 2018-10-16 清华大学 一种钩状结构寡肽及其应用
CN110139930B (zh) * 2016-12-28 2023-08-25 三井化学株式会社 突变型腈水合酶及其应用
KR20190049109A (ko) 2017-11-01 2019-05-09 (주)대화정공 풀리 제조방법
KR102146408B1 (ko) 2019-04-08 2020-08-21 (주)대화정공 단류선 개선을 위해 압연롤러를 이용한 자동차 댐퍼풀리 제조방법
KR102068615B1 (ko) 2019-04-08 2020-01-21 (주)대화정공 자동차 댐퍼풀리 제조방법
CN111690675B (zh) * 2019-08-01 2022-07-19 安徽瑞邦生物科技有限公司 一种表达腈水合酶突变体的重组菌及其制备方法和应用
CN112522245A (zh) * 2020-12-09 2021-03-19 江南大学 一种腈水合酶氨基酸基序的改造及其应用
CN113151234B (zh) * 2021-04-13 2022-08-12 浙江工业大学 腈水合酶赖氨酸突变体hba-k2h2r、编码基因及应用
CN113444714B (zh) * 2021-07-29 2022-06-21 浙江大学杭州国际科创中心 一种β亚基突变的腈水合酶突变体及其应用
CN114317507B (zh) * 2021-11-30 2024-07-02 清华大学 腈水合酶突变体及其应用
KR20250011936A (ko) * 2022-06-30 2025-01-22 미쓰이 가가쿠 가부시키가이샤 변이형 나이트릴 하이드라타제, 그 변이형 나이트릴 하이드라타제를 코딩하는 핵산, 그 핵산을 포함하는 벡터 및 형질 전환체, 그 변이형 나이트릴 하이드라타제의 제조 방법, 및 아마이드 화합물의 제조 방법
CN115772514B (zh) * 2022-11-03 2025-03-04 无锡新晨宇生物工程有限公司 一种腈水合酶底物通道氨基酸基序的改造用于肉桂酰胺的制备
EP4600368A1 (en) * 2024-02-09 2025-08-13 Adisseo France S.A.S. A method for enzymatic production of an amide compound

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH09275978A (ja) * 1996-02-14 1997-10-28 Mitsui Toatsu Chem Inc 新規なニトリルヒドラターゼ

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DD274631A5 (de) * 1987-09-18 1989-12-27 Kk Verfahren zur biologischen herstellung von amiden
AU627648B2 (en) 1990-02-28 1992-08-27 Teruhiko Beppu Dna fragment encoding a polypeptide having nitrile hydratase activity, a transformant containing the gene and a process for the production of amides using the transformant
JPH0856684A (ja) 1994-08-16 1996-03-05 Mitsui Toatsu Chem Inc 微生物によるアミド化合物の製造法
CN1243825C (zh) * 1996-02-14 2006-03-01 三井化学株式会社 新型腈水合酶
JP3408737B2 (ja) 1998-03-16 2003-05-19 三井化学株式会社 ニトリルヒドラターゼの活性化に関与するタンパク質及びそれをコードする遺伝子

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH09275978A (ja) * 1996-02-14 1997-10-28 Mitsui Toatsu Chem Inc 新規なニトリルヒドラターゼ

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
AKIMASA MIYANAGA,ET AL: "Crystal Structure of Cobalt-Containing Nitrile Hydratase", 《BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112322606A (zh) * 2020-11-20 2021-02-05 江南大学 一种腈水合酶突变体及其应用

Also Published As

Publication number Publication date
US20070009985A1 (en) 2007-01-11
AU2010202683A1 (en) 2010-07-15
KR20050089972A (ko) 2005-09-09
EP1586637A4 (en) 2008-03-19
WO2004056990A1 (ja) 2004-07-08
AU2003289338A1 (en) 2004-07-14
KR100819228B1 (ko) 2008-04-03
KR20070087054A (ko) 2007-08-27
EP1586637A1 (en) 2005-10-19
AU2010202683B2 (en) 2012-01-19
KR20070110146A (ko) 2007-11-15
JP2004194588A (ja) 2004-07-15
KR20070007393A (ko) 2007-01-15
EP1586637B1 (en) 2014-02-26
US7595184B2 (en) 2009-09-29
KR100785548B1 (ko) 2007-12-12
CN101892211A (zh) 2010-11-24
KR100825147B1 (ko) 2008-04-24
CN1729288A (zh) 2006-02-01
KR20070011620A (ko) 2007-01-24
KR100794001B1 (ko) 2008-01-10
CN103215242A (zh) 2013-07-24
KR100785549B1 (ko) 2007-12-12

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Application publication date: 20130515