CN102845199A - Artificial seed breeding technology of wild girald daphne bark - Google Patents
Artificial seed breeding technology of wild girald daphne bark Download PDFInfo
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Abstract
The invention discloses an artificial seed breeding technology of wild girald daphne bark. Seed breeding is finished by these steps of seed collecting, seed laminating, raising seedling, large-field transporting and planting, and field management. By breeding the girald daphne bark by the steps, the rate of emergence of the girald daphne bark can stably achieve over 71.3%, the seedling rate after lamination can achieve over 66%, and the seeding rate after careful selection can achieve over 85%. The successful research of the seed breeding technology of the wild girald daphne bark marks that artificial breeding and planting of the wild girald daphne bark can be completely achieved.
Description
Technical field
The invention belongs to the seed growing technology, particularly wild girald daphne bark's artificial seed raising technology.
Background technology
The girald daphne bark, formal name used at school claim yellow winter daphne (
daphne giraldii Nitsche), the Thymelaeceae daphne plant, mainly be distributed in the ground such as Gansu, Sichuan, Qinghai.Mainly be distributed in careless slope, shrubbery, the border area of Qilian Mountain Area height above sea level more than 2000 in Gansu Province.The girald daphne bark is at present in wild state, and without the domestication, the seminal propagation rate is low, and plant division is cultivated extremely difficulty and survived.At present domestic girald daphne bark's research is focused mostly at aspects such as the chemical composition of root skin, stem skin, active components, propagation method research mainly contains tissue culture quick breeding research etc., but the rarely seen report of cultivating in a organized way fast numerous girald daphne bark's success.To yellow winter daphne seed biological property, promote the research such as sprouting, seed seedling-raising, domesticating and cultivating and break girald daphne bark's seed dormancy and the successful report of growing seedlings, there is not yet.
Summary of the invention
The purpose of this invention is to provide the strong dormancy of a kind of girald daphne bark's of breaking seed, realize domestication's plantation of wild girald daphne bark's medicinal material and wild girald daphne bark's artificial seed raising technology of scale demonstration plant.
For achieving the above object, wild girald daphne bark's artificial seed raising technology of the present invention is completed by following steps:
1. wild girald daphne bark's artificial seed raising technology, it is characterized in that: described this raising technology is to be completed by following steps,
one, seed collection
A. select healthy and strong plant as the seed collecting maternal plant, carry out girald daphne bark's seed collection.Win gently while gathering that the fully rubescent girald daphne bark in surface is full, ripening fruits, by the timely airing of girald daphne bark's fruit of gathering, avoid heating rotten;
B. remove pulp
Fruit is broken the rubbing of girald daphne bark's fruit, then be placed in clear water and wash by rubbing with the hands, wash in a pan and remove the impurity such as pulp, pericarp, after cleaning, seed is poured in clear water, drag for the flat grain of the sky floated on the surface, the seed sunk is pulled out, drains away the water; Seed is put to cool place, ventilation, dry place natural air drying;
C. seed stores
The seeds of abundant drying, in ventilative carton, is put to cool place, drying, ventilation preservation;
two, earthing of seeds
A
.the seed pretreatment
A. seed cleaning
First get girald daphne bark's seed and sieve, after removing residual pulp, pericarp and other impurity, seed is placed in to salt solution, stir fast after 2 minutes and drag for the flat grain of the sky floated on the surface, rinse immediately drain well with clear water;
B. seed disinfection
Liquor potassic permanganate with 0.2% soaks selected seed 20min, with clear water, rinses for several times, drains away the water rear standby;
B. earthing of seeds
A. ditching
The selection of land gesture is higher, and draining is good, leeward in the shade local ditching.The degree of depth of ditch is in principle below frozen soil layer, more than subterranean water level.Furrow width 80cm, the length of ditch is depending on the quantity of seed.
B. seed covering
The seed covering degree of depth is between 60cm--120cm, and the seed covering time is that annual October 10 was to October 30.First at the thick wet sand of bottom of trench paving one deck 20cm, then by the seed after sterilization and clean wet river sand by volume 1 ︰ 3 mix, the river sand humidity requirement is held can be agglomerating, pine can scatter and be as the criterion, at dusk put into successively ditch, above cover the wet sand of 30cm left and right, then earthing makes top be ridge-shaped;
C. get kind
Dig out seed April next year after seed covering, go after sand standby with screen cloth sieve;
three, grow seedlings
A. culture facility is prepared:
Adopt 50 specification cave, hole dishes;
B. put bed
The wide 115cm-120cm in seedbed, length 40-50m, leveling makes real bed surface, and then lower berth one deck mulch film lays the cave dish;
C. sterilization
Seedbed adds water 25～30kg spray disinfectant with 200～300 milliliters of 40% formalin.1000 times of immersion bubble sterilizations more than 1 hour of potassium permanganate for the dish of cave, then sabot after the clear water flushing;
D. sabot and sowing
A. sabot
First matrix is mixed to humidity before sabot, hand is held agglomerating unclamping and is not fallen apart for well, Yi Bian the cave dish of then matrix being packed into is rear, with scraper plate, from the cave dish, scrapes to another side, and after sabot, each lattice chamber is high-visible, not with defeating matrix.On put a little plank, with have gentle hands is light, press one time, make the degree of depth reach the sowing requirement;
B. sowing
In annual April～sowing in May, select after lamination is processed the seed program request of germinateing or showing money or valuables one carries unintentionally and put in seedbed after the seedling medium of Pan Zhong， upper berth, cave, water subsequently permeable;
E. seedling management
A. temperature treatment
25 ℃～30 ℃ of the temperature day before yesterday of emerging, night, temperature was more than 12 ℃; The rear reduction temperature of emerging, summer high temperature needs sunshading and temperature reducing season;
B. illumination management
After 60% seed top soil, remove the covering on the dish of cave at dusk, enter normal management, late May is to early June, along with temperature raises, illumination strengthens, covered with sunshade net on culture facility, morning with afternoon temperature relatively remove sunshade net when low and see light, noon covered with sunshade net;
C. water and fertilizer management
Keep higher soil humidity before emerging after sowing, be advisable with 80～90%.Front 7～the 10d of field planting, suitably control moisture, water while watering and contain in the more matrix of root system, general matrix is with till watering not Wang Shui, water of the backsight weather condition of just having emerged 2d, after late May, water water every day one time, after seedling age reaches 30 days, impose weekly nutrient solution once, this nutrient solution is the potassium dihydrogen phosphate that the concentration urea that is 0.2% and concentration are 0.2%;
D. Index of The Healthy Seedlings
Height of seedling 6-10cm, the thick 0.25cm of stem left and right, 8-11 sheet leaf, dark green leaf, well developed root system is milky, anosis pityriasis simplex trace;
four, field-transplanting field planting
A. wholely
Excavate the horizontal ditch of dark 50cm, footpath 30cm, ditch, apart from being 50cm, will be shown earthen backfill after digging, and with the immature soil, build the ridge;
B. field planting
A. field planting time
Give birth to then seedling generally in late August to the early September field planting, as adopt biennial seedling, can be selected in field planting mid-May in spring.
B. field planting trench backfill
First with broken straw rod, mix a little mellow soil field planting trench backfill to 15～20cm is dark, every mu of cow dung 5m that executes again farmyard manure and become thoroughly decomposed
3left and right, pour water after last backfill farmland soil moved in to improve the original is full to ditch, makes soil in ditch sink real;
C. seedling transplant planting
Select height of seedling 7-10cm, the thick 0.2cm of stem left and right, dark green leaf, the 9-11 sheet leaf of having grown, well developed root system is creamy white, and line-spacing 50cm, spacing in the rows 50 cm field planting are pressed in girald daphne bark's seedling 1000 strains of anosis pityriasis simplex trace; Before field planting, seedling is carried out to classification and plant, during field planting, first with No. 6 ABT rooting powder solution 200ppm, dip in root, excavate field planting ditch soil, seedling is implanted in soil to soil layer around compacting with upright posture; In operating process, note damaging the seedling root system.Bury the native degree of depth and all bury and be advisable with root, fill with vola water after transplant planting;
five, field is managed and protected
Transplant then and to fill with foot winter water on November 5, Second Year was filled with a spring water April 5, loosened the soil in time, artificial weeding, and Mays 20 are in conjunction with pouring water and enrich a secondary pollutant quick-acting fertilizer for the second time.
Adopt above-mentioned steps to breed the girald daphne bark, can make stable the reaching more than 71.3% of girald daphne bark's percentage of seedgermination, the seed planting percent after lamination is processed reaches more than 66%, and after selected, planting percent reaches more than 85%.The success of wild girald daphne bark (yellow winter daphne) seed growing technical research, indicate that wild girald daphne bark carries out the artificial breeding plantation and can realize fully.Along with the extensive use of this achievement in research, will produce very significant Social benefit and economic benefit, and the artificial planting research of natural crude drugs in imminent danger is played to certain enlightenment and exemplary role.The research of wild girald daphne bark's artificial seed raising technology is conducive to the sustainable use of the required natural resources of Chinese medicinal materials of characteristic medical product, is conducive to the benign development of Special Traditional Chinese Medicine industry
;wild girald daphne bark's seed growing technical research obtains important breakthrough, is conducive to ecological protection;
The research success of girald daphne bark's (yellow winter daphne) seed growing technology, the optimization that is conducive to girald daphne bark's medical material quanlity improves.Utilize wild girald daphne bark (yellow winter daphne) seed growing technology, can be the scaled artificial planting girald daphne bark strong technical support is provided, plant Demonstration Base and Technique Popularizing application by enterprise, can drive local Cotton Varieties by Small Farming Households, can effectively promote the local Cropping structure adjustment of Wuwei, farmers' income increases; The research of wild girald daphne bark's seed growing technology breaks through, and to domestication's plantation of high height above sea level shrub natural crude drugs, provides effective way and scientific method.To the protection of natural crude drugs in imminent danger with bred extremely important enlightenment effect, great to the Chinese Medicine Industry Significance of Sustainable Development.
" research of wild girald daphne bark's artificial seed raising technology "; the equal tool major and immediate significances in aspect such as the science and technology demonstration that optimization and the natural crude drugs domestication of Development of Traditional Chinese Medicine, wild resource protection, ecological protection, agricultural restructuring, medical material quanlity are planted and promotion, remarkable economic and social benefits.
Embodiment
one, seed collection
1. girald daphne bark's seed provenance is determined
Through the Academy of Medical Sciences, Gansu Province drug research in one's power the provincial enterprise technique center scientific research personnel of Gansu Tai Kang Pharmaceutical Co determine, Qilian Mountain Area girald daphne bark's ripening fruits is as girald daphne bark's seed provenance.
2. girald daphne bark's seed collection time and collecting location
Girald daphne bark's seed collection time is on August 20th, 2010.Collecting location is boolean's intelligence grassland, the Qilian mountains under the township of Tianzhu Qilian.
3. girald daphne bark's seed collection
Select healthy and strong plant as the seed collecting maternal plant, carry out girald daphne bark's seed collection.Win gently while gathering that the fully rubescent girald daphne bark in surface is full, ripening fruits.By the timely airing of girald daphne bark's fruit of gathering, avoid heating to rot.Gather altogether girald daphne bark's fruit 150kg.
4. removal pulp
4.1 girald daphne bark's fruit is rubbed fruit is broken with hand rubbing, then be placed in clear water and wash by rubbing with the hands, wash in a pan and remove the impurity such as pulp, pericarp.After cleaning, seed is poured in clear water, dragged for the flat grain of the sky floated on the surface, the seed sunk is pulled out, drains away the water.
4.2 seed is put to cool place, ventilation, dry place natural air drying.Must plant 20kg after drying.
5. seed stores
The seeds of abundant drying, in ventilative carton, is put to cool place, drying, ventilation preservation.
two, earthing of seeds
1. seed pretreatment
1.1 seed cleaning
First get girald daphne bark's seed 10kg and sieve, after removing residual pulp, pericarp and other impurity, seed is placed in to salt solution, stir fast after 2 minutes and drag for the flat grain of the sky floated on the surface, rinse immediately drain well with clear water.
1.2 seed disinfection
Liquor potassic permanganate with 0.2% soaks selected seed 20min, with clear water, rinses for several times, drains away the water rear standby.
2. earthing of seeds
2.1 the lamination time
On October 22nd, 2010, lamination was processed seed.The lamination method adopts the trench storage method.
2.2 lamination is processed
2.2.1 ditching
The selection of land gesture is higher, and draining is good, leeward in the shade local ditching, and digging the wide X of long X is the ditch of 80cmX80cmX120cm deeply.
2.2.2 seed covering
The seed covering degree of depth is 40cm, 60cm, 80cm, 100 cm respectively.Each degree of depth is buried 2kg girald daphne bark seed.First at the thick wet sand of bottom of trench paving one deck 20cm, then by the seed after sterilization and clean wet river sand by volume 1 ︰ 3 mix, the river sand humidity requirement is held can be agglomerating, pine can scatter and be as the criterion.At dusk put into successively ditch, above cover the wet sand of 30 cm left and right, then earthing makes top be ridge-shaped.
2.2.3 get kind
Dig out seed on April 17th, 2011, the different depth seed carries out mark and deposits separately.With screen cloth sieve, go after sand standby.
three, grow seedlings
1. culture facility is prepared:
Adopt 50 specification cave, hole dishes.
2. seedling medium is selected:
Select the ecological seedling medium of producing with oasis farming research institute, Ningxia lucky chance company, Shouguang, Shandong and other places in the west of a river institute west of a river.
3. put bed
The length of two cave dishes of the wide 115cm-120cm(in seedbed), length 40-50m, leveling makes real bed surface, and then lower berth one deck mulch film lays the cave dish.
4. sterilization
Seedbed adds water 25～30kg spray disinfectant with 200～300 milliliters of 40% formalin.1000 times of immersion bubble sterilizations more than 1 hour of potassium permanganate for the dish of cave, then sabot after the clear water flushing.
5. sabot and sowing
5.1 sabot
First matrix is mixed to humidity before sabot, hand is held agglomerating unclamping and is not fallen apart for well, Yi Bian scrape to another side from the cave dish with scraper plate after the cave dish of then matrix being packed into.After sabot, each lattice chamber is high-visible, not with defeating matrix.On put a little plank, with have gentle hands is light, press one time, make the degree of depth reach the sowing requirement.
5.2 sowing
In sowing on April 18th, 2011, select after lamination is processed the seed program request of germinateing or showing money or valuables one carries unintentionally and put in seedbed after the seedling medium of Pan Zhong， upper berth, cave, water subsequently permeable.The seed of the different trench storage lamination degree of depth is seeded into respectively in the dish of different caves, 3000 of the planting seeds of each lamination degree of depth, and visible marking.
6. seedling management
6.1 temperature treatment
25 ℃～30 ℃ of the temperature day before yesterday of emerging, night, temperature was more than 12 ℃; The rear reduction temperature of emerging, summer high temperature needs sunshading and temperature reducing season.
6.2 illumination management
After 60% seed top soil, remove the covering on the dish of cave at dusk, enter normal management.Late May is to early June, along with temperature raises, illumination strengthens, covered with sunshade net on culture facility, morning with afternoon temperature relatively remove sunshade net when low and see light, noon covered with sunshade net.
6.3 water and fertilizer management
Keep higher soil humidity before emerging after sowing, be advisable with 80～90%.Front 7～the 10d of field planting, suitably control moisture.Water while watering and contain in the more matrix of root system, general matrix is with till watering not Wang Shui, water of the backsight weather condition of just having emerged 2d, and after late May, water water every day one time.After seedling age reaches 30 days, impose weekly nutrient solution (potassium dihydrogen phosphate of 0.2% urea+0.2%) once.
6.4 Index of The Healthy Seedlings
Height of seedling 6-10cm, the thick 0.25cm of stem left and right, 8-11 sheet leaf, dark green leaf, well developed root system is milky, anosis pityriasis simplex trace.
7. girald daphne bark's seedling is added up (table one)
Girald daphne bark's seeding ratio of table one, different depth trench storage lamination
| The trench storage degree of depth | Sowing seed number (grain) | Emerge and count (strain) | Emergence rate (%) |
| 40cm | 3000 | 1980 | 66.00 |
| 60cm | 3000 | 2175 | 72.50 |
| 80cm | 3000 | 2210 | 73.67 |
| 100cm | 3000 | 2280 | 76.00 |
four, field-transplanting field planting
1. the transplant planting soil is selected
The selection draining is good, and the soil texture be take the lawn of sandy loam and is field planting ground.
2. wholely
Excavate the horizontal ditch of dark 50cm, footpath 30cm, the ditch distance is 50cm.To show earthen backfill after digging, with the immature soil, build the ridge.
3. field planting
3.1 the field planting time
In field planting on September 3 in 2011.
3.2 field planting trench backfill
First with broken straw rod, mix a little mellow soil field planting trench backfill to 15～20cm is dark, then the cow dung 1m that executes farmyard manure and become thoroughly decomposed
3left and right, pour water after last backfill soil moved in to improve the original is full to ditch, makes soil in ditch sink real.
3.3 seedling transplant planting
Select height of seedling 7-10cm, the thick 0.2cm of stem left and right, dark green leaf, the 9-11 sheet leaf of having grown, well developed root system is creamy white, and line-spacing 50cm, spacing in the rows 50 cm field planting are pressed in girald daphne bark's seedling 1000 strains of anosis pityriasis simplex trace.Before field planting, seedling is carried out to classification and plant, during field planting, first with No. 6 ABT rooting powder solution 200ppm, dip in root, excavate field planting ditch soil, seedling is implanted in soil to soil layer around compacting with upright posture.In operating process, note damaging the seedling root system.Burying the native degree of depth all buries and is advisable with root.Fill with vola water after transplant planting.
5. field is managed and protected
Transplant and filled with then foot winter water on November 5, a spring water of Second Year filling on April 5.Loosen the soil in time, artificial weeding, Mays 20 are in conjunction with pouring water and enrich a secondary pollutant quick-acting fertilizer for the second time.
6. the girald daphne bark who transplants land for growing field crops plants shoot survival percent statistics (table two)
table twothe girald daphne bark who transplants land for growing field crops plants the shoot survival percent statistics
| Time | Plant height (cm) | Stem thick (cm) | Launch the number of sheets (cm) | The strain number | Survival rate (%) |
| 2011.9.3 | 7.5 | 0.21 | 9 | 1000 | —— |
| 2012.7.5 | 11.2 | 0.56 | 12 | 9750 | 97.5 |
Annotate: plant height, footpath are slightly the mean of random searching 20 strains.
Claims (1)
1. wild girald daphne bark's artificial seed raising technology, it is characterized in that: described this raising technology is to be completed by following steps,
one, seed collection
A. select healthy and strong plant as the seed collecting maternal plant, carry out girald daphne bark's seed collection;
Win gently while gathering that the fully rubescent girald daphne bark in surface is full, ripening fruits, by the timely airing of girald daphne bark's fruit of gathering, avoid heating rotten;
B. remove pulp
Fruit is broken the rubbing of girald daphne bark's fruit, then be placed in clear water and wash by rubbing with the hands, wash in a pan and remove the impurity such as pulp, pericarp, after cleaning, seed is poured in clear water, drag for the flat grain of the sky floated on the surface, the seed sunk is pulled out, drains away the water; Seed is put to cool place, ventilation, dry place natural air drying;
C. seed stores
The seeds of abundant drying, in ventilative carton, is put to cool place, drying, ventilation preservation;
two, earthing of seeds
A
.the seed pretreatment
A. seed cleaning
First get girald daphne bark's seed and sieve, after removing residual pulp, pericarp and other impurity, seed is placed in to salt solution, stir fast after 2 minutes and drag for the flat grain of the sky floated on the surface, rinse immediately drain well with clear water;
B. seed disinfection
Liquor potassic permanganate with 0.2% soaks selected seed 20min, with clear water, rinses for several times, drains away the water rear standby;
B. earthing of seeds
A. ditching
The selection of land gesture is higher, and draining is good, leeward in the shade local ditching;
?the degree of depth of ditch in principle below frozen soil layer, more than subterranean water level, furrow width 80cm, the length of ditch is depending on the quantity of seed;
B. seed covering
The seed covering degree of depth is between 60cm--120cm, and the seed covering time is that annual October 10 was to October 30;
First at the thick wet sand of bottom of trench paving one deck 20cm, then by the seed after sterilization and clean wet river sand by volume 1 ︰ 3 mix, the river sand humidity requirement is held can be agglomerating, pine can scatter and be as the criterion, at dusk put into successively ditch, above cover the wet sand of 30cm left and right, then earthing makes top be ridge-shaped;
C. get kind
Dig out seed April next year after seed covering, go after sand standby with screen cloth sieve;
three, grow seedlings
A. culture facility is prepared:
Adopt 50 specification cave, hole dishes;
B. put bed
The wide 115cm-120cm in seedbed, length 40-50m, leveling makes real bed surface, and then lower berth one deck mulch film lays the cave dish;
C. sterilization
Seedbed adds water 25～30kg spray disinfectant with 200～300 milliliters of 40% formalin;
1000 times of immersion bubble sterilizations more than 1 hour of potassium permanganate for the dish of cave, then sabot after the clear water flushing;
D. sabot and sowing
A. sabot
First matrix is mixed to humidity before sabot, hand is held agglomerating unclamping and is not fallen apart for well, Yi Bian the cave dish of then matrix being packed into is rear, with scraper plate, from the cave dish, scrapes to another side, and after sabot, each lattice chamber is high-visible, not with defeating matrix;
On put a little plank, with have gentle hands is light, press one time, make the degree of depth reach the sowing requirement;
B. sowing
In annual April～sowing in May, select after lamination is processed the seed program request of germinateing or showing money or valuables one carries unintentionally and put in seedbed after the seedling medium of Pan Zhong， upper berth, cave, water subsequently permeable;
E. seedling management
A. temperature treatment
25 ℃～30 ℃ of the temperature day before yesterday of emerging, night, temperature was more than 12 ℃; The rear reduction temperature of emerging, summer high temperature needs sunshading and temperature reducing season;
B. illumination management
After 60% seed top soil, remove the covering on the dish of cave at dusk, enter normal management, late May is to early June, along with temperature raises, illumination strengthens, covered with sunshade net on culture facility, morning with afternoon temperature relatively remove sunshade net when low and see light, noon covered with sunshade net;
C. water and fertilizer management
Keep higher soil humidity before emerging after sowing, be advisable with 80～90%;
Front 7～the 10d of field planting, suitably control moisture, water while watering and contain in the more matrix of root system, general matrix is with till watering not Wang Shui, water of the backsight weather condition of just having emerged 2d, after late May, water water every day one time, after seedling age reaches 30 days, impose weekly nutrient solution once, this nutrient solution is the potassium dihydrogen phosphate that the concentration urea that is 0.2% and concentration are 0.2%;
D. Index of The Healthy Seedlings
Height of seedling 6-10cm, the thick 0.25cm of stem left and right, 8-11 sheet leaf, dark green leaf, well developed root system is milky, anosis pityriasis simplex trace;
four, field-transplanting field planting
A. wholely
Excavate the horizontal ditch of dark 50cm, footpath 30cm, ditch, apart from being 50cm, will be shown earthen backfill after digging, and with the immature soil, build the ridge;
B. field planting
A. field planting time
Give birth to then seedling generally in late August to the early September field planting, as adopt biennial seedling, can be selected in field planting mid-May in spring;
B. field planting trench backfill
First with broken straw rod, mix a little mellow soil field planting trench backfill to 15～20cm is dark, every mu of cow dung 5m that executes again farmyard manure and become thoroughly decomposed
3left and right, pour water after last backfill farmland soil moved in to improve the original is full to ditch, makes soil in ditch sink real;
C. seedling transplant planting
Select height of seedling 7-10cm, the thick 0.2cm of stem left and right, dark green leaf, the 9-11 sheet leaf of having grown, well developed root system is creamy white, and line-spacing 50cm, spacing in the rows 50 cm field planting are pressed in girald daphne bark's seedling 1000 strains of anosis pityriasis simplex trace; Before field planting, seedling is carried out to classification and plant, during field planting, first with No. 6 ABT rooting powder solution 200ppm, dip in root, excavate field planting ditch soil, seedling is implanted in soil to soil layer around compacting with upright posture; In operating process, note damaging the seedling root system;
Bury the native degree of depth and all bury and be advisable with root, fill with vola water after transplant planting;
five, field is managed and protected
Transplant then and to fill with foot winter water on November 5, Second Year was filled with a spring water April 5, loosened the soil in time, artificial weeding, and Mays 20 are in conjunction with pouring water and enrich a secondary pollutant quick-acting fertilizer for the second time.
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| CN103797931A (en) * | 2014-02-25 | 2014-05-21 | 甘肃凯源生物技术开发中心 | Method for breaking dormancy of daphne giraldii seeds |
| CN104081913A (en) * | 2014-06-10 | 2014-10-08 | 甘肃凯源生物技术开发中心 | Method for increasing germination rate of daphne giraldii nitsche seeds |
| CN106508580A (en) * | 2016-11-11 | 2017-03-22 | 江西天海科技发展集团有限公司 | Rubus-chingii seed breeding method |
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| CN103703890A (en) * | 2013-12-19 | 2014-04-09 | 甘肃凯源生物技术开发中心 | Determination method for viability of daphne giraldii seeds |
| CN103797931A (en) * | 2014-02-25 | 2014-05-21 | 甘肃凯源生物技术开发中心 | Method for breaking dormancy of daphne giraldii seeds |
| CN103797931B (en) * | 2014-02-25 | 2016-08-17 | 甘肃凯源生物技术开发中心 | One abolishes the seed dormant method of Daphne Giraldii Nitsche |
| CN104081913A (en) * | 2014-06-10 | 2014-10-08 | 甘肃凯源生物技术开发中心 | Method for increasing germination rate of daphne giraldii nitsche seeds |
| CN104081913B (en) * | 2014-06-10 | 2015-10-28 | 甘肃凯源生物技术开发中心 | A kind of method improving D.giraldii Nitsche percentage of seedgermination |
| CN106508580A (en) * | 2016-11-11 | 2017-03-22 | 江西天海科技发展集团有限公司 | Rubus-chingii seed breeding method |
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| CN102845199B (en) | 2014-07-02 |
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